mRNA_P-wetherbeei_contig121.2.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig121.2.1
Unique NamemRNA_P-wetherbeei_contig121.2.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Match: K5X9W3_PHACS (Rhomboid domain-containing protein n=1 Tax=Phanerochaete carnosa (strain HHB-10118-sp) TaxID=650164 RepID=K5X9W3_PHACS)

HSP 1 Score: 78.6 bits (192), Expect = 1.550e-12
Identity = 57/169 (33.73%), Postives = 94/169 (55.62%), Query Frame = 2
Query:  440 RKHFTVSVSGV-SRRPWSMVLAAFAQEDTEHLLSNVFTLASVGPLSVAILGGGRTLALYAAGGVAGSAAALFYH-WLTDRRAVACLGASGAIYALMGFCFSAVPGAQMALLG-RSVPA---IQALLLNVAKDALMSARTRVDFAGHLGGLALGAALAQSSWWMGRSSRL 928
            RK+FTVS+  + S R W+++ A+F+ E   H+ +N FT   + P  +  LG    LALY  GG+  S  +L+++  + +R   +  GASGA+YA++ F     P  + AL G   +PA   +  + L    ++L  + TRVD AGH+GG+     LA   +++G+  R+
Sbjct:  153 RKNFTVSMQNLMSGRVWTLLTASFSHEGMMHIFTNAFTFYFMAPPVLQFLGTSGFLALYLGGGIFCSVVSLWWNNSVKNRPTYSSYGASGAVYAVISFFACVAPTTRFALFGVLPIPAWLFVTGVFLMDGYESLKDSHTRVDNAGHIGGI-----LAGIGYFLGKRFRI 316          
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Match: A0A1Y1I4Y3_KLENI (Rhomboid domain-containing protein n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1I4Y3_KLENI)

HSP 1 Score: 65.1 bits (157), Expect = 3.490e-8
Identity = 60/169 (35.50%), Postives = 85/169 (50.30%), Query Frame = 2
Query:  443 KHFTVSVSGVS-RRPWSMVLAAFAQEDTEHLLSNVFTLASVGPL--SVAILGGGRTLALYAAGGVAGSAAALFYHWL--------TDRRAV------ACLGASGAIYALMGFCFSAVPGAQMALLGRSVPA----IQALLLNVAKDALMSARTRVDFAGHLGGLALGAA 886
            KHF VSV+ +  RR  + V A F+  D  HL  N+  L S G +     + GGG  LALY AGG+ G  A    +W          DRRA       + LGASG++ AL+       P  ++ + G  VPA    I    L+++    ++  +R+ +AGHLGG A+GAA
Sbjct:   99 KHFQVSVASIEDRREHTAVTANFSHSDAGHLALNMLALHSFGRMIGHPRLFGGGGLLALYLAGGL-GCVAGHCLYWSYFVPWKQGVDRRAYNARRTPSALGASGSVSALLTCSILLNPSQKLLVYGLPVPAWLYGIFVTALDLSGAIGVNHGSRIGYAGHLGGAAVGAA 266          
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Match: A0A1I3GHN1_9ACTN (Membrane associated serine protease, rhomboid family n=2 Tax=Nocardioides psychrotolerans TaxID=1005945 RepID=A0A1I3GHN1_9ACTN)

HSP 1 Score: 62.8 bits (151), Expect = 2.050e-7
Identity = 54/151 (35.76%), Postives = 79/151 (52.32%), Query Frame = 2
Query:  485 WSMVLAAFAQEDTEHLLSNVFTLASVGPLSVAILGGGRTLALYAAGGVAGSAAALFYHWLTDRRAVACLGASGAIYALMGFCFSAVPGAQMALLGRSVPAIQALL----LNVAKDALMSARTRVDFAGHLGGLALGAALAQSSWWMGRSSR 925
            W +V + FA  +  H+  N+  L + GP     LG  R LALY   G++GSA   F +WL+D R+ A LGASGAI+ LMG        A + +  +    +Q+LL    +NVA   L S  + + + GHLGG   G A+  +  +  R+ R
Sbjct:  110 WQLVTSMFAHVEIWHIAFNMLALYAFGPQLEVALGRARFLALYLLSGLSGSA---FVYWLSDERS-ATLGASGAIFGLMG--------ALLVVAYKVGGNVQSLLTWIGINVAITVLGS--SFISWQGHLGGFVGGVAITAALVYAPRARR 246          
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Match: UPI001F21972C (rhomboid family intramembrane serine protease n=1 Tax=Corynebacterium uropygiale TaxID=1775911 RepID=UPI001F21972C)

HSP 1 Score: 61.6 bits (148), Expect = 2.640e-7
Identity = 55/137 (40.15%), Postives = 72/137 (52.55%), Query Frame = 2
Query:  482 PWSMVLAAFAQEDTEHLLSNVFTLASVGPLSVAILGGGRTLALYAAGGVAGSAAALFYHWLTDRRAVACLGASGAIYALMGFCFSAVPGAQMALLGRSVPAIQALLLNVAKDALMSARTRVDFAGHLGGLALGAALA 892
            P   + A F   D +HLL N+  L  VG  +    G  R LALY AGG+A SAA L++  LT       LGASGAI+ALM           ++L   + P +  L +NV    L+S+   +  AGHLGGLA GA +A
Sbjct:   58 PLRGITALFMHVDIQHLLLNMVMLWFVGGATEIAFGSARFLALYLAGGLASSAAILYFQPLTPT-----LGASGAIFALMAILIVIYHQKGLSL---ATP-LTLLGINVVGTFLVSS---ISVAGHLGGLAGGAIIA 182          
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Match: A0A7K0WEX6_9ACTN (Rhomboid family intramembrane serine protease n=2 Tax=root TaxID=1 RepID=A0A7K0WEX6_9ACTN)

HSP 1 Score: 61.2 bits (147), Expect = 6.080e-7
Identity = 51/150 (34.00%), Postives = 73/150 (48.67%), Query Frame = 2
Query:  446 HFTVSVSGVSRRPWSMVLAAFAQEDTEHLLSNVFTLASVGPLSVAILGGGRTLALYAAGGVAGSAAALFYHWLTDRRAVACLGASGAIYALMGFCFSAVPGAQMALLGRSVPAIQALL-LNVAKDALMSARTRVDFAGHLGGLALGAALA 892
            +F+ +    +  PW M+ AAF      HLL N+FTL   G +   +LG  R LALY      GS A LF+         + +GASGAIY LMG  F  +       +G     +  L+ +N+    L      + +  H+GGLA+GAA+A
Sbjct:   88 YFSYTPLATTIAPWQMLTAAFLHGSWLHLLFNMFTLYIFGQVLEPMLGRARFLALYLVAAFGGSVAVLFF----SNPVSSVVGASGAIYGLMGAYFVLLRS-----VGERAGQLTGLIAINLIFSFLSPG---ISWQAHIGGLAIGAAVA 225          
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Match: UPI000825D4F2 (rhomboid family intramembrane serine protease n=1 Tax=Herbiconiux solani TaxID=661329 RepID=UPI000825D4F2)

HSP 1 Score: 61.2 bits (147), Expect = 8.080e-7
Identity = 53/156 (33.97%), Postives = 75/156 (48.08%), Query Frame = 2
Query:  449 FTVSVSGVSRRPWSMVLAAFAQEDTE--------HLLSNVFTLASVGPLSVAILGGGRTLALYAAGGVAGSAAALFYHWLTDRRAVACLGASGAIYALMGFCFSAVPGAQMALLGRSVPAIQALLLNVAKDALMSARTRVDFAGHLGGLALGAALA 892
            +T+  +G    PW M+ + FA             H+L N++TL  +GP    +LG GR LALY   G  GS A  F    +   AV  LGASGAI+ LMG  F         L G S   +  +++N+A   ++S    + +  HLGGL  G  +A
Sbjct:  112 YTIPATGAPLEPWRMITSMFAHSPVSAQNPFGIFHILLNMYTLFVLGPSIERMLGKGRFLALYLIAGFGGSVAVAFL--ASPLSAV--LGASGAIFGLMGAYFII----NRHLGGSSAQIVVLVVINLAFGFVVSG---ISWQAHLGGLIAGGLVA 256          
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Match: A0A6L5K561_9CHLR (Rhomboid family intramembrane serine protease n=1 Tax=Dehalococcoidia bacterium TaxID=2026734 RepID=A0A6L5K561_9CHLR)

HSP 1 Score: 59.7 bits (143), Expect = 8.500e-7
Identity = 54/171 (31.58%), Postives = 87/171 (50.88%), Query Frame = 2
Query:  371 LITTNVVVFLSRWLLYGNDPWSWRKHFTVSVSGVSRRPWSMVLAAFAQEDTEHLLSNVFTLASVGPLSVAILGGGRTLALYAAGGVAGSAAALFYHWLTDRRAVACLGASGAIYALMGFCFSAVPGAQMALLGRSVPAIQALLLNVAKD-ALMSARTRVDFAGHLGGLALG 880
            LI  N+++F+   +++  +P      F +S      RPW++V + F      H+L+N+ TL   G     +LG G+ L +Y AGG+ GS   + Y  L    ++A +GASGAI+AL G    AV   ++ ++   +PA   L + +     + S    V +  H GGLALG
Sbjct:   16 LIAINLLMFVVTIIIHRTNPNFMYYTFGLSQVTFLDRPWTLVTSMFIHAGVWHILANMVTLYFFGSYLNRLLGTGKFLIIYFAGGILGS---IVYLLLAPSYSIA-IGASGAIFALGGVL--AVMRPRLRVIVFPIPAPLPLWIAIIGIFVVFSFLPNVAWQAHAGGLALG 180          
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Match: A0A3N4Q0K0_9BACT (Rhomboid family intramembrane serine protease n=1 Tax=Chitinophaga lutea TaxID=2488634 RepID=A0A3N4Q0K0_9BACT)

HSP 1 Score: 60.8 bits (146), Expect = 2.120e-6
Identity = 55/190 (28.95%), Postives = 92/190 (48.42%), Query Frame = 2
Query:  362 TELLITTNVVVFL--SRWLLYGNDPWSWRKHFTVSVSGVSR--------RPWSMVLAAFAQEDTEHLLSNVFTLASVGPLSVAILGGGRTLALYAAGGVAGSAAALFYHWLTDRRAVACLGASGAIYALMGFCFSAVPGAQMALLGRSVPAIQ--ALLLNVA-----KDALMSARTRVDFAGHLGGLALG 880
            T +LI  NV+++L  +  +L+    W W+   TV     +         +PW ++ A+F   D  HL  N++ +   G     +LG  R LA+Y    +A S A+L+++ +T       LGASGA++ L+GF F+        LL + V   +  ALL+++        A +  +T  D   H+GGL +G
Sbjct:  149 TPILIVLNVLIYLVLTNKMLFAGSSW-WQADVTVLEQAGANFKPLTLFGQPWRLLAASFLHADVMHLFFNLYGIMIGGTYLEPLLGRWRFLAVYLLCALAASIASLWWYDITPT-----LGASGAVFGLLGFIFTL-------LLRKFVEPFERRALLISIGIYLAFSLATIFMQTNFDHGAHIGGLLMG 325          
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Match: A0A094P9B4_9ACTN (Rhomboid domain-containing protein n=1 Tax=actinobacterium acMicro-1 TaxID=1504316 RepID=A0A094P9B4_9ACTN)

HSP 1 Score: 58.9 bits (141), Expect = 2.140e-6
Identity = 47/132 (35.61%), Postives = 66/132 (50.00%), Query Frame = 2
Query:  362 TELLITTNVVVFLSRW---------LLYGNDPWSWRKHFTVSVSGVSRRPWSMVLAAFAQEDTE--HLLSNVFTLASVGPLSVAILGGGRTLALYAAGGVAGSAAALFYHWLTDRRAVACLGASGAIYALMG 724
            T +LI  NV V+L +          LLY   P+     F  + +GV   PW M+ A FA   T   H+L N+FTL   G +  ++LG  R + LY   G+AGS   L++         + +GASGAI+ LMG
Sbjct:    2 TYVLIAINVFVYLLQLIPGINLTAVLLY--SPYYSLGEF--AAAGVPYEPWRMITATFAHSPTSFLHILFNMFTLWIFGQVLESMLGRARFITLYLIAGLAGSLGVLYFDLGLGLEFTSVVGASGAIFGLMG 129          
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Match: A0A2N5KN23_9ACTN (Rhomboid family intramembrane serine protease n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A2N5KN23_9ACTN)

HSP 1 Score: 58.9 bits (141), Expect = 2.470e-6
Identity = 46/135 (34.07%), Postives = 64/135 (47.41%), Query Frame = 2
Query:  479 RPWSMVLAAFAQEDTEHLLSNVFTLASVGPLSVAILGGGRTLALYAAGGVAGSAAALFYHWLTDRRAVACLGASGAIYALMGFCFSAVPGAQMALLGRSVPAIQALLLNVAKDALMS-ARTRVDFAGHLGGLALG 880
            +PW +V + F      HLL N+ +L  +G  + A  G GR  A+Y   G+AG  A L++   T   A   +GASGAI+ L+G  F      +        P I  LL   A +  +  +   V  A HLGGLA G
Sbjct:   55 QPWRLVTSIFLHSGITHLLLNMLSLYFLGSFAEATFGRGRFFAMYGLSGIAGGLAYLYWSVFTGAIAQPAVGASGAIFGLLGGVFGYA--IRRGTFSARDPVIGQLLFLTALNLFLGFSIPNVSNAAHLGGLAGG 187          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig121.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
K5X9W3_PHACS1.550e-1233.73Rhomboid domain-containing protein n=1 Tax=Phanero... [more]
A0A1Y1I4Y3_KLENI3.490e-835.50Rhomboid domain-containing protein n=1 Tax=Klebsor... [more]
A0A1I3GHN1_9ACTN2.050e-735.76Membrane associated serine protease, rhomboid fami... [more]
UPI001F21972C2.640e-740.15rhomboid family intramembrane serine protease n=1 ... [more]
A0A7K0WEX6_9ACTN6.080e-734.00Rhomboid family intramembrane serine protease n=2 ... [more]
UPI000825D4F28.080e-733.97rhomboid family intramembrane serine protease n=1 ... [more]
A0A6L5K561_9CHLR8.500e-731.58Rhomboid family intramembrane serine protease n=1 ... [more]
A0A3N4Q0K0_9BACT2.120e-628.95Rhomboid family intramembrane serine protease n=1 ... [more]
A0A094P9B4_9ACTN2.140e-635.61Rhomboid domain-containing protein n=1 Tax=actinob... [more]
A0A2N5KN23_9ACTN2.470e-634.07Rhomboid family intramembrane serine protease n=1 ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig121contigP-wetherbeei_contig121:5398..6619 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start1
Seed ortholog104355.XP_007862500.1
PFAMsRhomboid
Model size1222
Max annot lvl4751|Fungi
KEGG koko:K09650
Hectar predicted targeting categoryother localisation
Exons1
Evalue9.45e-11
EggNOG OGsCOG0705@1|root,KOG2980@2759|Eukaryota,3A5ZS@33154|Opisthokonta,3Q6XA@4751|Fungi,3V1S0@5204|Basidiomycota,229G5@155619|Agaricomycetes,3H56E@355688|Agaricomycetes incertae sedis
EC3.4.21.105
DescriptionRhomboid-domain-containing protein
Cds size765
COG categoryT
BRITEko00000,ko01000,ko01002,ko03029
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185937.7707024-UTR-P-wetherbeei_contig121:5397..56371683185937.7707024-UTR-P-wetherbeei_contig121:5397..5637Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig121 5398..5637 -
1683185937.7991405-UTR-P-wetherbeei_contig121:6402..66191683185937.7991405-UTR-P-wetherbeei_contig121:6402..6619Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig121 6403..6619 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185937.7873323-CDS-P-wetherbeei_contig121:5637..64021683185937.7873323-CDS-P-wetherbeei_contig121:5637..6402Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig121 5638..6402 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig121.2.1prot_P-wetherbeei_contig121.2.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig121 5638..6402 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig121.2.1

>prot_P-wetherbeei_contig121.2.1 ID=prot_P-wetherbeei_contig121.2.1|Name=mRNA_P-wetherbeei_contig121.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=255bp
MEFTDYFLLSTARLQKRRYLGLLGFWFRVPELPFADEKDIAAFADANATE
LLITTNVVVFLSRWLLYGNDPWSWRKHFTVSVSGVSRRPWSMVLAAFAQE
DTEHLLSNVFTLASVGPLSVAILGGGRTLALYAAGGVAGSAAALFYHWLT
DRRAVACLGASGAIYALMGFCFSAVPGAQMALLGRSVPAIQALLLNVAKD
ALMSARTRVDFAGHLGGLALGAALAQSSWWMGRSSRLLSWGAGSGSIPSA
LWWW*
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mRNA from alignment at P-wetherbeei_contig121:5398..6619-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig121.2.1 ID=mRNA_P-wetherbeei_contig121.2.1|Name=mRNA_P-wetherbeei_contig121.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1222bp|location=Sequence derived from alignment at P-wetherbeei_contig121:5398..6619- (Phaeothamnion wetherbeei SAG_119_79)
ACGGGATGGGCAGGGAGACGGCGGCGGCGACCGTCCGCCGAGCTCAAGCA GGAGGCGACTCCTCTCGCTCGCGGCGATCGTCTTTGCGCTCGCAATCTCT AGACCGTCTCGGGAATCCTTCGTTGCATACCTCCAAGCGACGCGTGGCAA CTCCAGTACAGGTGTTCGATGGCTAGGGCTTCTGCAACCTGGACTCGACG CATTGCAAGAGCGGTTCATGGAGTTTACGGATTATTTTCTGCTAAGCACT GCGCGATTGCAGAAGCGACGATACCTCGGCTTGCTGGGCTTTTGGTTTCG CGTGCCCGAGCTGCCGTTCGCCGACGAGAAGGACATTGCTGCGTTTGCAG ACGCCAATGCCACGGAATTGCTCATCACGACCAACGTGGTCGTCTTCCTC TCGCGGTGGCTACTTTACGGAAACGACCCATGGTCGTGGCGCAAGCACTT CACGGTCTCGGTTTCCGGCGTTTCACGCCGCCCCTGGAGCATGGTGCTGG CCGCTTTCGCGCAGGAGGACACCGAACATCTCCTCTCCAATGTCTTCACC CTCGCCTCCGTCGGCCCGCTCTCCGTCGCCATCCTCGGTGGCGGCCGCAC GCTCGCGCTGTACGCCGCCGGCGGTGTGGCTGGCAGCGCGGCGGCGCTGT TCTACCACTGGCTAACGGACCGCCGCGCCGTGGCATGCCTCGGCGCGAGC GGCGCCATCTACGCGCTCATGGGCTTCTGCTTTTCGGCCGTGCCGGGCGC GCAGATGGCGCTGCTTGGCCGCAGTGTCCCGGCTATCCAGGCGCTCCTGC TGAACGTGGCGAAGGACGCCCTTATGAGCGCCCGAACCCGCGTCGACTTT GCGGGCCATCTGGGCGGTTTGGCGCTCGGCGCGGCCCTGGCACAGAGTAG CTGGTGGATGGGACGGTCCAGTCGGTTGCTGTCGTGGGGGGCCGGCAGCG GCAGCATCCCCTCTGCTTTGTGGTGGTGGTGAGCGAGTCATTGTTAGTCT TGGGTTGTGCAGTTGTGCGTTTAATCGTGAGCGAGGAAAGGATAGGTCTA AAGTTGGGAGTGTGTTGGGAAACACTCGTTCCGTTTCCCCTCCTCTTTCA TGGCAGTCAGATACACTAAGACCAGAAGCGCGGCATGCGAGAGTTGTAAA TAGATCACAATCACAGCTGTATTCCGTATTCCGGCTCGCAAGTATTTTAC AGATAAGAGATGATTCAGTAAG
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Coding sequence (CDS) from alignment at P-wetherbeei_contig121:5398..6619-

>mRNA_P-wetherbeei_contig121.2.1 ID=mRNA_P-wetherbeei_contig121.2.1|Name=mRNA_P-wetherbeei_contig121.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=765bp|location=Sequence derived from alignment at P-wetherbeei_contig121:5398..6619- (Phaeothamnion wetherbeei SAG_119_79)
ATGGAGTTTACGGATTATTTTCTGCTAAGCACTGCGCGATTGCAGAAGCG
ACGATACCTCGGCTTGCTGGGCTTTTGGTTTCGCGTGCCCGAGCTGCCGT
TCGCCGACGAGAAGGACATTGCTGCGTTTGCAGACGCCAATGCCACGGAA
TTGCTCATCACGACCAACGTGGTCGTCTTCCTCTCGCGGTGGCTACTTTA
CGGAAACGACCCATGGTCGTGGCGCAAGCACTTCACGGTCTCGGTTTCCG
GCGTTTCACGCCGCCCCTGGAGCATGGTGCTGGCCGCTTTCGCGCAGGAG
GACACCGAACATCTCCTCTCCAATGTCTTCACCCTCGCCTCCGTCGGCCC
GCTCTCCGTCGCCATCCTCGGTGGCGGCCGCACGCTCGCGCTGTACGCCG
CCGGCGGTGTGGCTGGCAGCGCGGCGGCGCTGTTCTACCACTGGCTAACG
GACCGCCGCGCCGTGGCATGCCTCGGCGCGAGCGGCGCCATCTACGCGCT
CATGGGCTTCTGCTTTTCGGCCGTGCCGGGCGCGCAGATGGCGCTGCTTG
GCCGCAGTGTCCCGGCTATCCAGGCGCTCCTGCTGAACGTGGCGAAGGAC
GCCCTTATGAGCGCCCGAACCCGCGTCGACTTTGCGGGCCATCTGGGCGG
TTTGGCGCTCGGCGCGGCCCTGGCACAGAGTAGCTGGTGGATGGGACGGT
CCAGTCGGTTGCTGTCGTGGGGGGCCGGCAGCGGCAGCATCCCCTCTGCT
TTGTGGTGGTGGTGA
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