mRNA_P-wetherbeei_contig11685.2.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig11685.2.1
Unique NamemRNA_P-wetherbeei_contig11685.2.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Match: D7FMM2_ECTSI (GE20757 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FMM2_ECTSI)

HSP 1 Score: 188 bits (478), Expect = 7.630e-55
Identity = 86/152 (56.58%), Postives = 117/152 (76.97%), Query Frame = 3
Query:  363 TAAAMWAWIVSFPITIATFSRV-AAPLGLWGKLAFAGFGIGFAVETVADYQKFVFKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLLRGPAQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVARTPALVP 815
            T  AMWAW+VS P+T+A FS   A P+G+ G         G AVETVADYQKF FKN P+N  +++ +GL+S+ R+PNYLGEM VWW++LG ALP LRGP ++ALGFASPAF+T+L+++VSG+PMLEQQH+++YG +P +RE+   TP ++P
Sbjct:  153 TIQAMWAWVVSLPVTLANFSPARAVPMGVGGWACLGLAATGLAVETVADYQKFQFKNDPDNKGKFMTSGLWSLSRHPNYLGEMGVWWAILGVALPALRGPGRVALGFASPAFITALIMYVSGVPMLEQQHDEKYGGDPRYREWKENTPMILP 304          
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Match: A0A835Z1P4_9STRA (Uncharacterized protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835Z1P4_9STRA)

HSP 1 Score: 154 bits (388), Expect = 1.540e-41
Identity = 72/150 (48.00%), Postives = 104/150 (69.33%), Query Frame = 3
Query:  372 AMWAWIVSFPITIATFSRVAAP--LGLWGKLAFAGFGIGFAVETVADYQKFVFKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLLRGPAQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVARTPALVP 815
            A WAW+VS P+T+A F+ VA+   LG+ G++A   F  GFA E +AD+QK  FK  P N  +WID G++SV R+PNY GE+++W SL  A++P LRGP Q+ +G  SP F++ L+L+VSGIP+LE +  K +G++  + +Y   TP LVP
Sbjct:  144 AAWAWVVSLPVTMANFNPVASTQRLGMGGRVALLTFAGGFAFEAIADWQKRSFKKNPANKGKWIDQGVWSVSRHPNYFGELLIWSSLFFASVPALRGPRQVLIGAVSPLFISGLILYVSGIPLLEDKMNKDHGQDEKYLKYKETTPLLVP 293          
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Match: A0A0G4EDP8_VITBC (S5A_REDUCTASE domain-containing protein n=1 Tax=Vitrella brassicaformis (strain CCMP3155) TaxID=1169540 RepID=A0A0G4EDP8_VITBC)

HSP 1 Score: 142 bits (359), Expect = 1.040e-36
Identity = 70/164 (42.68%), Postives = 99/164 (60.37%), Query Frame = 3
Query:  363 TAAAMWAWIVSFPITIATFSRVAAPLGLWGKLAFAGFGIGFAVETVADYQKFV----------FKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLLR---GPAQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVARTPALVP 815
            TA  +W W+   P T+A +S+ A  L  W     A F +GFA ET+AD QK+            K K +   +W+D GL+ +CRYPNY GE+ VW  L   A+P L    GP  +ALG  SPAF++SLLLFVSG+P+ E++ +KR+G +P ++EY   TP ++P
Sbjct:  191 TAQTVWCWLSLLPSTVAVYSKAATSLTRWDAFGIALFSLGFAFETIADNQKWAKKQREMKGEAAKGKGDKAAKWMDEGLWRLCRYPNYFGEICVWSGLYAVAVPALYQAYGPKMVALGALSPAFVSSLLLFVSGVPLAEKRADKRFGDDPQYQEYKKSTPLIIP 354          
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Match: I0YVW6_COCSC (DUF1295-domain-containing protein n=1 Tax=Coccomyxa subellipsoidea (strain C-169) TaxID=574566 RepID=I0YVW6_COCSC)

HSP 1 Score: 140 bits (352), Expect = 1.980e-36
Identity = 76/152 (50.00%), Postives = 104/152 (68.42%), Query Frame = 3
Query:  363 TAAAMWAWIVSFPITIATFSRVAAPLGLWGKLAFAGFGIGFAVETVADYQKFVFKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLLRGP-AQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVARTPALVP 815
            TA ++W WIV  PIT++     AAPLG  G +A AGF  GFA+ETVAD QKF FK+   + D+++D+GL+++CR+PNY GE+++W SL    L    G  A+     ASPAF   LLL+VSGIP LE+ HEKRYG++ A+++Y A T  L+P
Sbjct:  129 TAQSLWGWIVLLPITVSQTLTPAAPLGPLGWIAAAGFLAGFAIETVADLQKFYFKSA--HPDKFMDSGLFALCRHPNYFGEILLWTSL--TVLAGTHGVFAKHPWIIASPAFTIFLLLYVSGIPTLEKSHEKRYGKDAAYKDYKASTNLLLP 276          
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Match: A0A841RBT5_9SPIO (Steroid 5-alpha reductase family enzyme n=1 Tax=Spirochaeta isovalerica TaxID=150 RepID=A0A841RBT5_9SPIO)

HSP 1 Score: 133 bits (335), Expect = 4.460e-34
Identity = 67/145 (46.21%), Postives = 90/145 (62.07%), Query Frame = 3
Query:  384 WIVSFPITIATFSRVAAPLGLW-GKLAFAGFGIGFAVETVADYQKFVFKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLLRGPAQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVARTPALVP 815
            W++  P+T     R   P  L+   + F  F IGF +E+++D+QKF FKN  +N   WI+ GL+   R+PNY GEM+VWW L  AA+P L G   L +    P F+T LLLFVSGIP+LE+  EK+YG NP +R Y  RT  L+P
Sbjct:  117 WVIMLPVTFFLTVRE-VPFNLYLHSIGFVLFAIGFIIESISDHQKFRFKNNEKNKGLWIENGLWKYSRHPNYFGEMLVWWGLFIAAIPALGGLKILTV--MGPVFITLLLLFVSGIPLLEKSAEKKYGGNPLYRSYRDRTSLLIP 258          
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Match: UPI000D1D0756 (uncharacterized protein LOC9647142 n=2 Tax=Selaginella moellendorffii TaxID=88036 RepID=UPI000D1D0756)

HSP 1 Score: 133 bits (334), Expect = 1.010e-33
Identity = 63/148 (42.57%), Postives = 89/148 (60.14%), Query Frame = 3
Query:  372 AMWAWIVSFPITIATFSRVAAPLGLWGKLAFAGFGIGFAVETVADYQKFVFKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLLRGPAQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVARTPALVP 815
            A W W VS P+TIA  S +  PL     + +  +  G  +E  +DYQK  FK   +N D+W D G +SV R+PNY GEM++WW +  AA P+ RG    A+    P FL ++LLF+SG+P+LE   +K+YG NP++R Y   T  L+P
Sbjct:  112 AAWVWTVSLPVTIANASDMDPPLEARDAIGWFLWICGIVIEMTSDYQKLKFKEDMDNRDKWCDIGFWSVSRHPNYFGEMLLWWGIFAAASPVFRGGQWAAI--FGPLFLMAILLFLSGLPLLEASADKKYGSNPSYRMYKETTSPLIP 257          
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Match: A0A0D3CB53_BRAOL (S5A_REDUCTASE domain-containing protein n=33 Tax=Brassicaceae TaxID=3700 RepID=A0A0D3CB53_BRAOL)

HSP 1 Score: 133 bits (334), Expect = 1.060e-33
Identity = 68/153 (44.44%), Postives = 91/153 (59.48%), Query Frame = 3
Query:  363 TAAAMWAWIVSFPITIATFS---RVAAPLGLWGKLAFAGFGIGFAVETVADYQKFVFKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLLRGPAQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVARTPALV 812
            T  A+W W VS P+T+   S   R+  P  + G   F     GF VE +AD QK +FKN+ EN  RW D GL+   R+PNY GEM++WW +  A+LP+L+G   L +    P FLT LL FVSGIP+LE+  +K+YG   A+R Y   T  L+
Sbjct:  109 TLQAVWVWTVSLPVTLVNASNGGRLFQPADVIGWTMFVA---GFLVEAIADQQKLLFKNRQENKGRWCDVGLWKFSRHPNYFGEMLLWWGIYVASLPVLKGAEYLVI--VGPIFLTLLLFFVSGIPLLEESADKKYGNLGAYRHYKKTTSPLI 256          
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Match: A0A446R8L9_TRITD (S5A_REDUCTASE domain-containing protein n=7 Tax=Pooideae TaxID=147368 RepID=A0A446R8L9_TRITD)

HSP 1 Score: 131 bits (329), Expect = 1.420e-33
Identity = 65/148 (43.92%), Postives = 87/148 (58.78%), Query Frame = 3
Query:  372 AMWAWIVSFPITIATFSRVAAPLGLWGKLAFAGFGIGFAVETVADYQKFVFKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLLRGPAQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVARTPALVP 815
            A+W W VS P+TI   S     +     + +  + IG AVE +AD QK  FKN P N  +W + GL+S  R+PNY GEM +WW +  A+ P+L G   L +    P FLT LLLFVSGIP+LE   +KR+GR+  +R Y   T  L+P
Sbjct:   55 AVWVWTVSLPVTIVNASSRNPSIEARDIIGWIMWAIGLAVEAIADQQKLKFKNSPSNRGKWCNVGLWSYTRHPNYFGEMFLWWGVFVASAPVLSGAEWLVI--LGPIFLTLLLLFVSGIPLLESSADKRFGRSEEYRTYKKTTSPLIP 200          
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Match: A8J7X5_CHLRE (Predicted protein (Fragment) n=1 Tax=Chlamydomonas reinhardtii TaxID=3055 RepID=A8J7X5_CHLRE)

HSP 1 Score: 129 bits (325), Expect = 2.720e-33
Identity = 65/151 (43.05%), Postives = 92/151 (60.93%), Query Frame = 3
Query:  363 TAAAMWAWIVSFPITIATFSRVAAPLGLWGKLAFAGFGIGFAVETVADYQKFVFKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLLRGPAQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVARTPALVP 815
            T  A+W ++   P+      R   PLG    L  A +G+G A+E  AD+QK  +K++PEN  R+ID GL+S+ R+PNY GEM++WW +     P   G A   +  ASP  +  LL +VSG+P+LEQ  E+R+G  PAF+EY ART  L+P
Sbjct:   48 TMQAVWVFVSLLPVLCINGGRAPVPLGPLDMLGIAVYGLGMALEVTADWQKAAWKSRPENKGRFIDEGLWSLSRHPNYCGEMMIWWGVFLTCAPGF-GAAWQYVSVASPVVVMLLLRYVSGVPLLEQMGEQRWGTEPAFQEYKARTNLLLP 197          
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Match: A0A835XRS5_9CHLO (S5A_REDUCTASE domain-containing protein n=1 Tax=Edaphochlamys debaryana TaxID=47281 RepID=A0A835XRS5_9CHLO)

HSP 1 Score: 133 bits (335), Expect = 3.580e-33
Identity = 68/155 (43.87%), Postives = 93/155 (60.00%), Query Frame = 3
Query:  351 MIDGTAAAMWAWIVSFPITIATFSRVAAPLGLWGKLAFAGFGIGFAVETVADYQKFVFKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLLRGPAQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVARTPALVP 815
            ++  TA A+W ++ S P+ I        PLG    +    + +GF  E VAD QK VFK KPEN  R+ID GL+S+ R+PNY GEM++WW +     P    P  LA    SP F   L+ FVSG+P+LE+  E+R+G+ PAF+EY ART  L+P
Sbjct:  202 LVAWTAQAVWVFLTSLPVLIINGMPSTRPLGPIDAIGITVWAVGFLTEVVADRQKAVFKAKPENKGRFIDEGLWSLSRHPNYFGEMMLWWGVFLTCAPGFSSPWHLA-SVVSPVFTFLLIRFVSGVPLLEKMGEQRWGQEPAFQEYKARTNMLLP 355          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig11685.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FMM2_ECTSI7.630e-5556.58GE20757 n=2 Tax=Ectocarpus TaxID=2879 RepID=D7FMM2... [more]
A0A835Z1P4_9STRA1.540e-4148.00Uncharacterized protein n=1 Tax=Tribonema minus Ta... [more]
A0A0G4EDP8_VITBC1.040e-3642.68S5A_REDUCTASE domain-containing protein n=1 Tax=Vi... [more]
I0YVW6_COCSC1.980e-3650.00DUF1295-domain-containing protein n=1 Tax=Coccomyx... [more]
A0A841RBT5_9SPIO4.460e-3446.21Steroid 5-alpha reductase family enzyme n=1 Tax=Sp... [more]
UPI000D1D07561.010e-3342.57uncharacterized protein LOC9647142 n=2 Tax=Selagin... [more]
A0A0D3CB53_BRAOL1.060e-3344.44S5A_REDUCTASE domain-containing protein n=33 Tax=B... [more]
A0A446R8L9_TRITD1.420e-3343.92S5A_REDUCTASE domain-containing protein n=7 Tax=Po... [more]
A8J7X5_CHLRE2.720e-3343.05Predicted protein (Fragment) n=1 Tax=Chlamydomonas... [more]
A0A835XRS5_9CHLO3.580e-3343.87S5A_REDUCTASE domain-containing protein n=1 Tax=Ed... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig11685contigP-wetherbeei_contig11685:623..1902 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start1
Seed ortholog2880.D7FMM2
PFAMsDUF1295
Model size815
Max annot lvl2759|Eukaryota
Hectar predicted targeting categorysignal peptide
Exons3
Evalue1.97e-57
EggNOG OGsCOG3752@1|root,KOG4650@2759|Eukaryota
Ec32 ortholog descriptionProtein of unknown function DUF1295
Ec32 orthologEc-23_000860.1
Descriptionoxidoreductase activity, acting on the CH-CH group of donors
Cds size465
COG categoryO
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185922.19437-CDS-P-wetherbeei_contig11685:622..8431683185922.19437-CDS-P-wetherbeei_contig11685:622..843Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig11685 623..843 -
1683185922.2060447-CDS-P-wetherbeei_contig11685:1089..13121683185922.2060447-CDS-P-wetherbeei_contig11685:1089..1312Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig11685 1090..1312 -
1683185922.215228-CDS-P-wetherbeei_contig11685:1531..15521683185922.215228-CDS-P-wetherbeei_contig11685:1531..1552Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig11685 1532..1552 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185922.224612-UTR-P-wetherbeei_contig11685:1552..19021683185922.224612-UTR-P-wetherbeei_contig11685:1552..1902Phaeothamnion wetherbeei SAG_119_79UTRP-wetherbeei_contig11685 1553..1902 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig11685.2.1prot_P-wetherbeei_contig11685.2.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig11685 623..1552 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig11685.2.1

>prot_P-wetherbeei_contig11685.2.1 ID=prot_P-wetherbeei_contig11685.2.1|Name=mRNA_P-wetherbeei_contig11685.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=155bp
MIDGTAAAMWAWIVSFPITIATFSRVAAPLGLWGKLAFAGFGIGFAVETV
ADYQKFVFKNKPENTDRWIDTGLYSVCRYPNYLGEMIVWWSLLGAALPLL
RGPAQLALGFASPAFLTSLLLFVSGIPMLEQQHEKRYGRNPAFREYVART
PALVP
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mRNA from alignment at P-wetherbeei_contig11685:623..1902-

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig11685.2.1 ID=mRNA_P-wetherbeei_contig11685.2.1|Name=mRNA_P-wetherbeei_contig11685.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1280bp|location=Sequence derived from alignment at P-wetherbeei_contig11685:623..1902- (Phaeothamnion wetherbeei SAG_119_79)
ATTGGTGGGTGCAATCTGGTCGGGTACGGACTTACCGTAACGCTCGAGAC GCACAAGCTCACCGACCTCGTCGGAGTTGGCGCATTTGTCGTCTCCGCCG CCGCGGCAAGCATCGTCAACGCCCCGGCACTGTTCGCTGGCGGCAAGGTG CAGCTCCGCCCGGTCCTGCTAAACGCGGCATTGGGTCTCTGGGGGGCGCG CCTGGCCAGCTATCTTTTCTACCGAGTGCTGAGCACTGGTGAGGACAAGC GGCTCGCGCCCTTCTTCCGCGAGAGGGGCGAGAAGTACTTCGATGCGAAG CGCTCCAACTTTCCGTTGAAGCTCGCCGGCTTCTGGACGATACAGGCGCG ATGATTGATGGGACTGCCGCAGTTGAAGTGGTTAATATGGCCTGAAGGGA ACAGAAGCGTCTGCCACAGCTACGTCCTCACCCTTCTACTTGTGGTGAAG TCTGCTTGTTAGATGCATCTGCCTGACCACTCTCGAAGGAGCAGGAGAAA TAGACTCGGCTGCAAAGGCAGCATAGAGGAAGAAAGGACGGCGCTCAAAA AGTTAACGCTTGGGAGACGTCTTTTCTCTTGCTGCGGCAGGCCATGTGGG CGTGGATCGTGTCCTTCCCCATCACGATCGCTACCTTCTCGCGGGTGGCG GCACCGCTCGGCCTCTGGGGAAAGCTGGCGTTCGCGGGCTTCGGAATCGG TTTTGCGGTCGAGACGGTGGCCGACTATCAGAAGTTCGTCTTCAAGAACA AGCCCGAGAACACGGACCGCTGGATAGACACGGGACTCTACAGCGTCTGT CGCTACCCCAACTGTGAGCAGATGGACCCCTTTCCGTGCCTTCTGACTTT GAGCGCTTGCATTCGGAGCTAAAATTTTGTTTGAGCGGTGTGAGGCAAGT GTCTTTATACGCCGCGATGCGTAAACTAGCAGGACTCGCGGCTGCCAGTA CGGTCGGTCGGACGACAGCGCTGTTACTGCTGCACGTTTTTAACACGCGC AGGAACTGCAGCACGACTCTTCCTTTCCCGTTGCATCGTTTCCGACGTTT TGACGTCAGACCTGGGCGAGATGATCGTGTGGTGGTCGCTGCTCGGAGCC GCGCTGCCGCTGCTCCGCGGGCCGGCGCAGCTGGCGCTAGGCTTCGCCTC GCCGGCGTTCCTGACGTCGCTGCTGCTTTTTGTGAGCGGCATCCCCATGC TGGAGCAGCAGCACGAGAAGCGCTATGGCAGGAACCCGGCCTTCCGGGAA TACGTGGCGCGGACGCCAGCACTCGTGCCG
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Coding sequence (CDS) from alignment at P-wetherbeei_contig11685:623..1902-

>mRNA_P-wetherbeei_contig11685.2.1 ID=mRNA_P-wetherbeei_contig11685.2.1|Name=mRNA_P-wetherbeei_contig11685.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=465bp|location=Sequence derived from alignment at P-wetherbeei_contig11685:623..1902- (Phaeothamnion wetherbeei SAG_119_79)
ATGATTGATGGGACTGCCGCAGCCATGTGGGCGTGGATCGTGTCCTTCCC
CATCACGATCGCTACCTTCTCGCGGGTGGCGGCACCGCTCGGCCTCTGGG
GAAAGCTGGCGTTCGCGGGCTTCGGAATCGGTTTTGCGGTCGAGACGGTG
GCCGACTATCAGAAGTTCGTCTTCAAGAACAAGCCCGAGAACACGGACCG
CTGGATAGACACGGGACTCTACAGCGTCTGTCGCTACCCCAACTACCTGG
GCGAGATGATCGTGTGGTGGTCGCTGCTCGGAGCCGCGCTGCCGCTGCTC
CGCGGGCCGGCGCAGCTGGCGCTAGGCTTCGCCTCGCCGGCGTTCCTGAC
GTCGCTGCTGCTTTTTGTGAGCGGCATCCCCATGCTGGAGCAGCAGCACG
AGAAGCGCTATGGCAGGAACCCGGCCTTCCGGGAATACGTGGCGCGGACG
CCAGCACTCGTGCCG
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