mRNA_P-wetherbeei_contig10505.2.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig10505.2.1
Unique NamemRNA_P-wetherbeei_contig10505.2.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
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BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Match: A0A835YJ67_9STRA (Vacuolar protein 14 C-terminal Fig4p binding-domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YJ67_9STRA)

HSP 1 Score: 192 bits (489), Expect = 1.580e-55
Identity = 100/126 (79.37%), Postives = 109/126 (86.51%), Query Frame = 1 
Query:    1 ITVLDSVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIKTSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSLALLGAIMHCISDAEPEIRAVAGQTNQ 378
            ITVLDSVPDINM DWLPDFLDGLFNMLSD NREIRQAAD ALAEFL+EIK S+FVEYGPMV+ILVGQC+SKER NRLTA+TWV EFI LGG+R +L  S  LL AIM CISD E +I AVAGQTN+
Sbjct:  208 ITVLDSVPDINMVDWLPDFLDGLFNMLSDGNREIRQAADGALAEFLREIKESAFVEYGPMVSILVGQCNSKERSNRLTAITWVQEFIALGGERLLLFYS-DLLSAIMQCISDPEDDICAVAGQTNR 332
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BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Match: F0YQT7_AURAN (Vac14_Fig4_bd domain-containing protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YQT7_AURAN)

HSP 1 Score: 181 bits (460), Expect = 6.630e-53
Identity = 92/120 (76.67%), Postives = 103/120 (85.83%), Query Frame = 1 
Query:   16 SVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIKTSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSLALLGAIMHCISDAEPEIRAVAGQTN 375
            SVPDINM DWLPDFLDGLFNMLSD+NREIRQAAD+A+A FL EIK S  VE+GPMV ILV QCH+KERFNRLTA+TWV EFIKLGGDR +L  S  LLG+IM+CISD++ EIR VAG TN
Sbjct:    1 SVPDINMLDWLPDFLDGLFNMLSDSNREIRQAADSAIAGFLNEIKKSPVVEFGPMVGILVNQCHNKERFNRLTAITWVKEFIKLGGDRLLLFYS-ELLGSIMYCISDSDLEIRQVAGYTN 119
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BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Match: A0A7S2XZF0_9STRA (Hypothetical protein (Fragment) n=2 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2XZF0_9STRA)

HSP 1 Score: 178 bits (451), Expect = 7.410e-53
Identity = 93/126 (73.81%), Postives = 105/126 (83.33%), Query Frame = 1 
Query:    1 ITVLDSVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIKTSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSLALLGAIMHCISDAEPEIRAVAGQTNQ 378
            ITVLDSVPDI M DWLPDFLDGLFNMLSD N EIRQAAD ALAEFL+EI+ + FVE+GPMV+ILVGQC+SKERFNRLTA+ WV EFI LG +R +L  S  LLGAIMHCISD E +IR  A +TN+
Sbjct:  127 ITVLDSVPDIIMIDWLPDFLDGLFNMLSDGNLEIRQAADLALAEFLREIRETDFVEFGPMVSILVGQCNSKERFNRLTAMKWVREFIVLGQERLLLFYS-ELLGAIMHCISDNEADIRRDAEETNE 251
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BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Match: D8LEZ3_ECTSI (Vac14_Fig4_bd domain-containing protein n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LEZ3_ECTSI)

HSP 1 Score: 185 bits (470), Expect = 1.710e-52
Identity = 93/126 (73.81%), Postives = 106/126 (84.13%), Query Frame = 1 
Query:    1 ITVLDSVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIKTSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSLALLGAIMHCISDAEPEIRAVAGQTNQ 378
            ITVLDSVPDINM DWLPDFLDGL NMLSD NREIRQAAD AL++FL+EI+ SSFV++GPMV ILVG C+SK+RFNRLTAV WVHEFIKLGG+R VL     LLGAI HCISD +P +R  AG+ N+
Sbjct:  204 ITVLDSVPDINMIDWLPDFLDGLLNMLSDGNREIRQAADTALSDFLKEIQASSFVDFGPMVPILVGHCNSKDRFNRLTAVQWVHEFIKLGGERLVLF-FWELLGAISHCISDTDPVVRERAGEANK 328
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BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Match: F0YIP2_AURAN (Vac14_Fig4_bd domain-containing protein (Fragment) n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0YIP2_AURAN)

HSP 1 Score: 185 bits (469), Expect = 1.920e-52
Identity = 95/125 (76.00%), Postives = 105/125 (84.00%), Query Frame = 1 
Query:    1 ITVLDSVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIKTSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSLALLGAIMHCISDAEPEIRAVAGQTN 375
            ITVLDSVPDINM DWLPDFLDGLFNMLSD+NREIRQAA +A+  FL EIK S+ VE+GPMV ILV QC +KERFNRLTA+TWV EFIKLGG R +L  S  LLGAIMHCISD++ EIR VAG TN
Sbjct:  206 ITVLDSVPDINMLDWLPDFLDGLFNMLSDSNREIRQAAGSAIGGFLIEIKKSTVVEFGPMVGILVSQCRNKERFNRLTAITWVQEFIKLGGSRLLLFYS-ELLGAIMHCISDSDTEIRQVAGYTN 329
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BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Match: A0A8K1CS78_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CS78_PYTOL)

HSP 1 Score: 180 bits (457), Expect = 1.750e-50
Identity = 92/125 (73.60%), Postives = 106/125 (84.80%), Query Frame = 1 
Query:    1 ITVLDSVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIKTSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSLALLGAIMHCISDAEPEIRAVAGQTN 375
            ITVLDSVPDI+M DWLP+FLDGLFNMLSD NREIRQAAD+ALAEFL+EIK +  V++G MV+ILVGQC+SKERFNRLTA+TWVHEF+ LG +R V   +  LL AIMHCISDAE EIR VA + N
Sbjct:  237 ITVLDSVPDIDMLDWLPEFLDGLFNMLSDGNREIRQAADSALAEFLREIKQTDHVDFGRMVDILVGQCNSKERFNRLTAITWVHEFVNLGKERLVAFYA-ELLAAIMHCISDAEHEIRQVAERAN 360
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BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Match: A0A2D4C2Y6_PYTIN (tRNA-5-taurinomethyluridine 2-sulfurtransferase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4C2Y6_PYTIN)

HSP 1 Score: 181 bits (460), Expect = 2.210e-50
Identity = 93/125 (74.40%), Postives = 106/125 (84.80%), Query Frame = 1 
Query:    1 ITVLDSVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIKTSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSLALLGAIMHCISDAEPEIRAVAGQTN 375
            ITVLDSVPDI+M DWLP+FLDGLFNMLSD NREIRQAAD+ALAEFL+EIK +  V+YG MV+ILVGQC+SKERFNRLTA+TWVHEF+ LG +R V   +  LL AIMHCISDAE EIR VA + N
Sbjct:  957 ITVLDSVPDIDMLDWLPEFLDGLFNMLSDGNREIRQAADSALAEFLREIKQTEHVDYGRMVDILVGQCNSKERFNRLTAITWVHEFVMLGKERLVAFYA-QLLAAIMHCISDAEHEIRQVAERAN 1080
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BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Match: A0A2P4XIT2_9STRA (VAC14 family protein n=2 Tax=Phytophthora palmivora var. palmivora TaxID=611791 RepID=A0A2P4XIT2_9STRA)

HSP 1 Score: 174 bits (441), Expect = 1.780e-49
Identity = 91/125 (72.80%), Postives = 105/125 (84.00%), Query Frame = 1 
Query:    1 ITVLDSVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIKTSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSLALLGAIMHCISDAEPEIRAVAGQTN 375
            ITVLDSVPDI+M DWLP+FLDGLFNMLSD NREIRQAAD+ALAEFL+EIK +  VE+G MV+ILVGQC+SKERFNRLTAV+WV EF+ LG ++ V   +  LL AIMHCISDAE EIR VA + N
Sbjct:  270 ITVLDSVPDIDMLDWLPEFLDGLFNMLSDGNREIRQAADSALAEFLREIKQTEDVEFGRMVDILVGQCNSKERFNRLTAVSWVQEFVNLGREKLVEFYA-DLLAAIMHCISDAEHEIRQVAERAN 393
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BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Match: A0A067BKZ9_SAPPC (Vac14_Fig4_bd domain-containing protein n=4 Tax=Saprolegniaceae TaxID=4764 RepID=A0A067BKZ9_SAPPC)

HSP 1 Score: 177 bits (448), Expect = 1.900e-49
Identity = 90/125 (72.00%), Postives = 102/125 (81.60%), Query Frame = 1 
Query:    1 ITVLDSVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIKTSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSLALLGAIMHCISDAEPEIRAVAGQTN 375
            ITVLDSVPDI M DWLP+FLDGLFNMLSD NREIRQAAD+AL EFL+EIK++ FV++GPMV+ILV QC SKERF RLTA TWVHEF+ LG +R V      LLGAI+HCISDAE EIR V  + N
Sbjct:  220 ITVLDSVPDIEMLDWLPEFLDGLFNMLSDGNREIRQAADSALTEFLREIKSTPFVDFGPMVHILVAQCQSKERFTRLTAATWVHEFVVLGKERLVRFYG-DLLGAILHCISDAETEIRIVGERAN 343
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BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Match: W2QV08_PHYPN (Vac14_Fig4_bd domain-containing protein n=6 Tax=Phytophthora parasitica TaxID=4792 RepID=W2QV08_PHYPN)

HSP 1 Score: 174 bits (441), Expect = 2.000e-49
Identity = 91/125 (72.80%), Postives = 105/125 (84.00%), Query Frame = 1 
Query:    1 ITVLDSVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIKTSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSLALLGAIMHCISDAEPEIRAVAGQTN 375
            ITVLDSVPDI+M DWLP+FLDGLFNMLSD NREIRQAAD+ALAEFL+EIK +  VE+G MV+ILVGQC+SKERFNRLTAV+WV EF+ LG ++ V   +  LL AIMHCISDAE EIR VA + N
Sbjct:   25 ITVLDSVPDIDMLDWLPEFLDGLFNMLSDGNREIRQAADSALAEFLREIKQTEDVEFGRMVDILVGQCNSKERFNRLTAVSWVQEFVNLGREKLVEFYA-DLLAAIMHCISDAEHEIRQVAERAN 148
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig10505.2.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835YJ67_9STRA1.580e-5579.37Vacuolar protein 14 C-terminal Fig4p binding-domai... [more]
F0YQT7_AURAN6.630e-5376.67Vac14_Fig4_bd domain-containing protein (Fragment)... [more]
A0A7S2XZF0_9STRA7.410e-5373.81Hypothetical protein (Fragment) n=2 Tax=Fibrocapsa... [more]
D8LEZ3_ECTSI1.710e-5273.81Vac14_Fig4_bd domain-containing protein n=2 Tax=Ec... [more]
F0YIP2_AURAN1.920e-5276.00Vac14_Fig4_bd domain-containing protein (Fragment)... [more]
A0A8K1CS78_PYTOL1.750e-5073.60Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A2D4C2Y6_PYTIN2.210e-5074.40tRNA-5-taurinomethyluridine 2-sulfurtransferase n=... [more]
A0A2P4XIT2_9STRA1.780e-4972.80VAC14 family protein n=2 Tax=Phytophthora palmivor... [more]
A0A067BKZ9_SAPPC1.900e-4972.00Vac14_Fig4_bd domain-containing protein n=4 Tax=Sa... [more]
W2QV08_PHYPN2.000e-4972.80Vac14_Fig4_bd domain-containing protein n=6 Tax=Ph... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig10505contigP-wetherbeei_contig10505:1177..2291 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog2880.D8LEZ3
Preferred nameVAC14
PFAMsVac14_Fab1_bd,Vac14_Fig4_bd
Model size408
Max annot lvl2759|Eukaryota
KEGG koko:K10875,ko:K15305
KEGG Pathwayko03440,ko05166,ko05203,map03440,map05166,map05203
Hectar predicted targeting categoryother localisation
GOsGO:0000139,GO:0000306,GO:0000322,GO:0000323,GO:0000324,GO:0000329,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005768,GO:0005769,GO:0005770,GO:0005773,GO:0005774,GO:0005794,GO:0005942,GO:0006629,GO:0006644,GO:0006650,GO:0006661,GO:0006793,GO:0006796,GO:0006950,GO:0006970,GO:0007154,GO:0007165,GO:0007267,GO:0008047,GO:0008150,GO:0008152,GO:0008610,GO:0008654,GO:0009058,GO:0009628,GO:0009893,GO:0009987,GO:0010008,GO:0010562,GO:0012505,GO:0016020,GO:0019207,GO:0019209,GO:0019220,GO:0019222,GO:0019637,GO:0019898,GO:0023052,GO:0030154,GO:0030234,GO:0030674,GO:0031090,GO:0031312,GO:0031323,GO:0031325,GO:0031410,GO:0031901,GO:0031902,GO:0031982,GO:0031984,GO:0032502,GO:0032991,GO:0033674,GO:0035032,GO:0038023,GO:0042325,GO:0042327,GO:0043085,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043549,GO:0044093,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044422,GO:0044424,GO:0044425,GO:0044431,GO:0044433,GO:0044437,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0045017,GO:0045165,GO:0045168,GO:0045937,GO:0046331,GO:0046474,GO:0046486,GO:0046488,GO:0048518,GO:0048522,GO:0048869,GO:0050789,GO:0050790,GO:0050794,GO:0050896,GO:0051174,GO:0051338,GO:0051347,GO:0051716,GO:0060089,GO:0060090,GO:0061695,GO:0065007,GO:0065009,GO:0070772,GO:0071704,GO:0090407,GO:0097708,GO:0098588,GO:0098772,GO:0098791,GO:0098796,GO:0098805,GO:0098852,GO:1901576,GO:1902494,GO:1990234
Exons3
Evalue2.81e-53
EggNOG OGsKOG0212@1|root,KOG0212@2759|Eukaryota
Descriptionphosphatidylinositol biosynthetic process
Cds size408
COG categoryL
BRITEko00000,ko00001,ko01000,ko03400
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185872.944662-CDS-P-wetherbeei_contig10505:1176..12811683185872.944662-CDS-P-wetherbeei_contig10505:1176..1281Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10505 1177..1281 -
1683185872.9562812-CDS-P-wetherbeei_contig10505:1719..18811683185872.9562812-CDS-P-wetherbeei_contig10505:1719..1881Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10505 1720..1881 -
1683185872.9650586-CDS-P-wetherbeei_contig10505:2150..22911683185872.9650586-CDS-P-wetherbeei_contig10505:2150..2291Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10505 2151..2291 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig10505.2.1prot_P-wetherbeei_contig10505.2.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig10505 1177..2291 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig10505.2.1

>prot_P-wetherbeei_contig10505.2.1 ID=prot_P-wetherbeei_contig10505.2.1|Name=mRNA_P-wetherbeei_contig10505.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=136bp
ITVLDSVPDINMTDWLPDFLDGLFNMLSDNNREIRQAADAALAEFLQEIK
TSSFVEYGPMVNILVGQCHSKERFNRLTAVTWVHEFIKLGGDRRVLSRSL
ALLGAIMHCISDAEPEIRAVAGQTNQVRQWQMAFPA
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mRNA from alignment at P-wetherbeei_contig10505:1177..2291-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig10505.2.1 ID=mRNA_P-wetherbeei_contig10505.2.1|Name=mRNA_P-wetherbeei_contig10505.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=1115bp|location=Sequence derived from alignment at P-wetherbeei_contig10505:1177..2291- (Phaeothamnion wetherbeei SAG_119_79)
ATCACGGTGCTGGACAGCGTGCCGGACATCAACATGACGGACTGGCTGCC
TGACTTCCTTGACGGCCTCTTCAACATGCTCTCCGACAATAACCGCGAGA
TCCGCCAGGCCGCCGACGCCGCCCTCGCTGAGTTCCTCCAGGTATGTATG
ACGGGCGAGTTTTGAAGAGTGTGCGGGGCTTTGGTCTGCCTTGTTGACCA
CTCACCTTTTCCTTGTTTCTGGTGCGTGGAGAGTTGATGCTTCCATTCGG
GGCTTGCGTGGCAGTGCTTGCGGGGTGGTATCGGCTGTTTTTCGCGTCAC
CTCGTTATGCCTGTTCTTGGCTCGTCTTCTCTGAAGCCGAACCCAATAGA
TCGCCGCCCTGCATATCTCTACCGCACCGTTCTGTTGCATTGCCCTGCTC
CCGCCTGCAGGAGATCAAGACGTCGAGTTTTGTCGAGTACGGGCCTATGG
TGAACATCCTTGTGGGGCAGTGCCACTCCAAGGAGCGCTTCAACCGACTC
ACCGCCGTCACCTGGGTCCACGAGTTCATCAAGCTCGGCGGGGACAGGCG
GGTCCTTAGTCGCTCCCTGGCAGTCGCTGGTCACTGGCCGCTCCGTTTAC
CGGCTGTCCTGGCAGCTCTTTCGCTGTATCTTTCCTTCTTCCCTTTCCTG
TCTTTGCTGCTGAGTTTTCTGGCTGAGCTCCGGTATATCGCTTGGCTTGA
TGATTGGCGTTTGCAATAACGTCGCATTGTCCTTGCTCTTCTTTTTATCT
GCCGTCCTGTTGCTTACTGGCCTTTTGTGAACGCTGCTCCTTCTTCATTG
TCGCTCGGCGGCAACTGTGGCGGCGGCGGCGGCGGCGGTGACAACGGCGG
CGGCGGCGGCGGCGGCGGCGGCGGCACTGGTGACTGGCGGCGACGATTGC
GACGGCATTTGCGGCCGCAACTAGCGCATTGATGGCGATAATGGCGGTCG
CGGCTGCTCACATTCATGGTCACGGCGTCGCAAAACAGACTGCTGCTGTT
CTACTCGGAGTTGCTGGGCGCCATCATGCACTGCATCAGCGACGCCGAGC
CGGAGATCCGCGCAGTCGCTGGGCAAACCAACCAGGTGAGGCAGTGGCAG
ATGGCTTTTCCGGCC
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Coding sequence (CDS) from alignment at P-wetherbeei_contig10505:1177..2291-

>mRNA_P-wetherbeei_contig10505.2.1 ID=mRNA_P-wetherbeei_contig10505.2.1|Name=mRNA_P-wetherbeei_contig10505.2.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=408bp|location=Sequence derived from alignment at P-wetherbeei_contig10505:1177..2291- (Phaeothamnion wetherbeei SAG_119_79)
ATCACGGTGCTGGACAGCGTGCCGGACATCAACATGACGGACTGGCTGCC
TGACTTCCTTGACGGCCTCTTCAACATGCTCTCCGACAATAACCGCGAGA
TCCGCCAGGCCGCCGACGCCGCCCTCGCTGAGTTCCTCCAGGAGATCAAG
ACGTCGAGTTTTGTCGAGTACGGGCCTATGGTGAACATCCTTGTGGGGCA
GTGCCACTCCAAGGAGCGCTTCAACCGACTCACCGCCGTCACCTGGGTCC
ACGAGTTCATCAAGCTCGGCGGGGACAGGCGGGTCCTTAGTCGCTCCCTG
GCATTGCTGGGCGCCATCATGCACTGCATCAGCGACGCCGAGCCGGAGAT
CCGCGCAGTCGCTGGGCAAACCAACCAGGTGAGGCAGTGGCAGATGGCTT
TTCCGGCC
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