mRNA_P-wetherbeei_contig10480.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig10480.1.1
Unique NamemRNA_P-wetherbeei_contig10480.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Match: D7FQB3_ECTSI (Glucosidase 2 subunit beta n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FQB3_ECTSI)

HSP 1 Score: 86.7 bits (213), Expect = 2.650e-17
Identity = 49/120 (40.83%), Postives = 63/120 (52.50%), Query Frame = 1
Query:   25 DLGPGKILAALARRRFALDTGTFRYEVAPFVEVKQHHTLIGRWRGFG-------GSTNHLLWEFDDGQHCPGAGRREARVKLVCGTEHRLVSAEEPAKCRYGLILETPIACCDADVVEAE 363
            DLG G   AALA +   L+ G + Y + PF E KQ    +GRWRG+G       G     +  FD G  C    +R   V + CG+E  LV+AEE     Y L LETP+AC +A + EAE
Sbjct:  610 DLGEGSRFAALAEKCLDLEEGGYLYSLCPFKEAKQDKHSLGRWRGWGPGGRGRAGRGRDRVMLFDGGSRCHNNKQRSCEVSVQCGSEDALVAAEEIEIGTYRLRLETPLACTEAHLAEAE 729          
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Match: A0A6H5JF29_9PHAE (MRH domain-containing protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JF29_9PHAE)

HSP 1 Score: 84.0 bits (206), Expect = 2.180e-16
Identity = 49/123 (39.84%), Postives = 65/123 (52.85%), Query Frame = 1
Query:   25 DLGPGKILAALARRRFALDTGTFRYEVAPFVEVKQHHTLIGRWRGFG-------GSTNHLLWEFDDGQHCPGAGRREARVKLVCGTEHRLVSAEEPAKCRYGLILETPIACCDADVVEAEMAM 372
            DLG G   AALA +   L+ G + Y + PF + KQ    +GRWRG+G       G   + +  FD G  C    +R   V + CG E  LV+AEE     Y L LETP+AC +A V EAE+ +
Sbjct:  364 DLGEGSRFAALAGKCLDLEEGGYLYSLCPFKDAKQDKHSLGRWRGWGTGGRGLPGRGRNRVMLFDGGSRCHNK-QRSCEVSVECGPEDALVAAEEIEIGTYHLRLETPLACTEAHVAEAELGL 485          
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Match: C4Y3D7_CLAL4 (Glucosidase 2 subunit beta n=2 Tax=Clavispora lusitaniae TaxID=36911 RepID=C4Y3D7_CLAL4)

HSP 1 Score: 82.0 bits (201), Expect = 9.920e-16
Identity = 44/121 (36.36%), Postives = 67/121 (55.37%), Query Frame = 1
Query:    7 EDWLRRDLGPGKILAALARRRFALDTGTFRYEVAPFVEVKQHHTLIGRWRGFGGSTNHLLWEFDDGQHCPGAGRREARVKLVCGTEHRLVSAEEPAKCRYGLILETPIACCDADVVEAEMA 369
            E+ +RR  G   IL ++     +   G + Y++     V Q +TL+GR+ GF  S+      F  G  C    +R A+V +VCG EH +VS  EP KC+Y ++LETP+ C  +D+ E E+A
Sbjct:  361 EETVRRSYGENDILRSVEGTWVSKKIGDYTYKIGFLDSVYQDNTLVGRFSGFDSSS----LTFSHGSKCWNGPQRSAKVDMVCGPEHVIVSVSEPEKCQYRILLETPLVC--SDLSEEELA 475          
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Match: K3X1Z1_GLOUD (Glucosidase 2 subunit beta n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3X1Z1_GLOUD)

HSP 1 Score: 80.5 bits (197), Expect = 3.700e-15
Identity = 38/118 (32.20%), Postives = 65/118 (55.08%), Query Frame = 1
Query:   22 RDLGPGKILAALARRRFALDTGTFRYEVAPFVEVKQHHTLIGRWRGFGGSTN----HLLWEFDDGQHCPGAGRREARVKLVCGTEHRLVSAEEPAKCRYGLILETPIACCDADVVEAE 363
            +D GP +   AL  +        + Y++  F +VKQ +TL+G+W  +G + N    +   +F  GQ C     R  +V+L CG E+ +VS +EP+ C Y ++L+TP+AC    + +A+
Sbjct:  495 KDYGPERAFFALNDQCIEKKIEKYTYKLCAFRDVKQDYTLLGKWEAWGSADNGDKDYARMQFIKGQKCWNGPERSVQVRLACGQENEIVSVDEPSTCVYEMLLQTPLACTQDVLAQAQ 612          
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Match: UPI0004F4B68A (protein kinase C substrate protein n=1 Tax=Trypanosoma grayi TaxID=71804 RepID=UPI0004F4B68A)

HSP 1 Score: 74.3 bits (181), Expect = 5.060e-13
Identity = 41/126 (32.54%), Postives = 63/126 (50.00%), Query Frame = 1
Query:    4 AEDWLRRDLGPGKILAALARRRFALDTGTFRYEVAPFVEVKQHHT------LIGRWRGFGGSTNHLLWE---------FDDGQHCPGAGRREARVKLVCGTEHRLVSAEEPAKCRYGLILETPIAC 336
            A+  L R +    I+ AL    F +D  ++ YE+ PF +  Q++        +GRW  F  ST + LW          F++G  C     R   V +VCG+E++LV AEEP+ C Y ++ +TP  C
Sbjct:  355 AQKELNRTINAEDIVKALEGECFTVDFMSYTYELCPFKDTHQYNKGTKSGPCMGRWGRFAEST-YSLWASTSDYTHMIFENGDRCWNGVHRSTDVHVVCGSENKLVQAEEPSMCHYNMVFQTPAVC 479          
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Match: A0A4P9XW18_9FUNG (Glucosidase 2 subunit beta n=1 Tax=Thamnocephalis sphaerospora TaxID=78915 RepID=A0A4P9XW18_9FUNG)

HSP 1 Score: 74.3 bits (181), Expect = 5.180e-13
Identity = 42/115 (36.52%), Postives = 59/115 (51.30%), Query Frame = 1
Query:   25 DLGPGKILAALARRRFALDTGTFRYEVAPFVEVKQHHTL------IGRWRGFG-----GSTNHLLWEFDDGQHCPGAGRREARVKLVCGTEHRLVSAEEPAKCRYGLILETPIAC 336
            D GP     A+  + FAL++  + Y V  F  VKQ          +GR+RG+      G + ++   ++DGQ+C     R   V L CGTE +++SAEEP KC Y L L TP  C
Sbjct:  388 DFGPDYRFGAIDEKCFALESHEYTYTVCMFDSVKQTRKGEYGEIDLGRFRGWDDAATDGESRYMKQRYEDGQYCWNGPNRSTEVLLECGTEEKVLSAEEPEKCVYRLRLTTPAVC 502          
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Match: A0A812L8W4_9DINO (Glucosidase 2 subunit beta n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812L8W4_9DINO)

HSP 1 Score: 73.2 bits (178), Expect = 1.010e-12
Identity = 39/113 (34.51%), Postives = 53/113 (46.90%), Query Frame = 1
Query:    4 AEDWLRRDLGPGKILAALARRRFALDTGTFRYEVAPFVEVKQHHTLIGRWRGFGGSTNHLLWEFDDGQHCPGAGRREARVKLVCGTEHRLVSAEEPAKCRYGLILETPIACCD 342
            AE    +D GPG     L  + F +    + Y V P+   KQ  T +G W GF      +L  F  G  C    +R   V L CG  + L S +EP+KC+Y  +L+TP  C D
Sbjct:  235 AEAAAEKDFGPGAAFYKLDGQCFDVKVQKYTYSVCPYGSAKQDQTSLGSWSGFQVEYTKML--FTGGATCWNGPQRTMTVTLECGATNALHSVDEPSKCQYSAVLQTPAVCDD 345          
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Match: UPI001C828109 (uncharacterized protein n=1 Tax=Scheffersomyces spartinae TaxID=45513 RepID=UPI001C828109)

HSP 1 Score: 70.9 bits (172), Expect = 8.030e-12
Identity = 35/107 (32.71%), Postives = 55/107 (51.40%), Query Frame = 1
Query:   16 LRRDLGPGKILAALARRRFALDTGTFRYEVAPFVEVKQHHTLIGRWRGFGGSTNHLLWEFDDGQHCPGAGRREARVKLVCGTEHRLVSAEEPAKCRYGLILETPIAC 336
            L R  G   I  AL   + + D G ++Y++A    + Q   L+G++  F      +L+ + +G  C     R AR+ LVCG  H ++S  EP KC Y + + +PIAC
Sbjct:  312 LNRYYGKDDIYRALNDIKISTDYGDYKYKIALLDSIHQDKVLVGKFANFDEHDESILY-YTNGSKCWNGPHRSARIILVCGKTHEIISISEPEKCEYTIEMTSPIAC 417          
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Match: A0A1J4KXZ0_9EUKA (Glucosidase 2 subunit beta n=1 Tax=Tritrichomonas foetus TaxID=1144522 RepID=A0A1J4KXZ0_9EUKA)

HSP 1 Score: 70.1 bits (170), Expect = 1.410e-11
Identity = 44/114 (38.60%), Postives = 61/114 (53.51%), Query Frame = 1
Query:   25 DLGPGKILAALARRRFALDTGTFRYEVAPFVEVKQHHTLIGRWRGFGGSTNHLLWEFDDGQHC-PGAGRREARVKLVCGTEHRLVSAEEPAKCRYGLILETPIACCDADVVEAE 363
            DL P ++L+   ++    + G   Y +    +VKQ H ++G ++   GST +    FD G HC      R   + LVCG+E+RLVSAEEP+ C Y  I ETP AC   DV   E
Sbjct:  275 DLVPPELLSLYGQK---FENG--EYTLKFLKKVKQPHNIVGFFKNMSGSTLY----FDGGDHCWKTEAPRNFELSLVCGSENRLVSAEEPSVCVYKGIFETPFACSTDDVENVE 379          
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Match: A0A068WMF9_ECHGR (Glucosidase 2 subunit beta n=3 Tax=Echinococcus TaxID=6209 RepID=A0A068WMF9_ECHGR)

HSP 1 Score: 66.6 bits (161), Expect = 2.460e-10
Identity = 42/129 (32.56%), Postives = 62/129 (48.06%), Query Frame = 1
Query:   25 DLGPGK---ILAALARRRFALDTGTFRYEVAPFVEVKQ---HH----TLIGRWRGF-GGSTNHLLWE--------------FDDGQHCPGAGRREARVKLVCGTEHRLVSAEEPAKCRYGLILETPIAC 336
            D GP K   +L   +     +    + Y +  F EV+Q   HH    TL+G W G+ G   + + W               +D G+ C     R  +VK+VCG ++RL+SAEEP++C Y + LETP  C
Sbjct:  287 DYGPDKGFMMLTEPSTGCLEVQNKEYIYSLCAFKEVRQRPRHHSTGGTLLGSWSGWVGHPEDSVHWSKEEKLAKLPYNEMLYDGGEQCWNGPSRSVKVKVVCGAQNRLMSAEEPSRCTYKMRLETPATC 415          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig10480.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FQB3_ECTSI2.650e-1740.83Glucosidase 2 subunit beta n=1 Tax=Ectocarpus sili... [more]
A0A6H5JF29_9PHAE2.180e-1639.84MRH domain-containing protein n=1 Tax=Ectocarpus s... [more]
C4Y3D7_CLAL49.920e-1636.36Glucosidase 2 subunit beta n=2 Tax=Clavispora lusi... [more]
K3X1Z1_GLOUD3.700e-1532.20Glucosidase 2 subunit beta n=1 Tax=Globisporangium... [more]
UPI0004F4B68A5.060e-1332.54protein kinase C substrate protein n=1 Tax=Trypano... [more]
A0A4P9XW18_9FUNG5.180e-1336.52Glucosidase 2 subunit beta n=1 Tax=Thamnocephalis ... [more]
A0A812L8W4_9DINO1.010e-1234.51Glucosidase 2 subunit beta n=1 Tax=Symbiodinium sp... [more]
UPI001C8281098.030e-1232.71uncharacterized protein n=1 Tax=Scheffersomyces sp... [more]
A0A1J4KXZ0_9EUKA1.410e-1138.60Glucosidase 2 subunit beta n=1 Tax=Tritrichomonas ... [more]
A0A068WMF9_ECHGR2.460e-1032.56Glucosidase 2 subunit beta n=3 Tax=Echinococcus Ta... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig10480contigP-wetherbeei_contig10480:1470..2331 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop0
Start0
Seed ortholog2880.D7FQB3
Preferred nameGNPTG
PFAMsPRKCSH-like,PRKCSH_1
Model size375
Max annot lvl2759|Eukaryota
KEGG koko:K04246,ko:K05546,ko:K08288,ko:K10087
KEGG ReactionR05980,R05981
KEGG Pathwayko00510,ko01100,ko01522,ko04141,ko04142,ko04915,map00510,map01100,map01522,map04141,map04142,map04915
KEGG ModuleM00073,M00074
Hectar predicted targeting categoryother localisation
GOsGO:0000139,GO:0000271,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005576,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005788,GO:0005794,GO:0005975,GO:0005976,GO:0006464,GO:0006486,GO:0006487,GO:0006491,GO:0006605,GO:0006622,GO:0006623,GO:0006793,GO:0006796,GO:0006807,GO:0006810,GO:0006886,GO:0006950,GO:0006952,GO:0007034,GO:0007041,GO:0008104,GO:0008150,GO:0008152,GO:0009058,GO:0009059,GO:0009100,GO:0009101,GO:0009605,GO:0009607,GO:0009617,GO:0009987,GO:0012505,GO:0015031,GO:0015833,GO:0016020,GO:0016051,GO:0016256,GO:0016310,GO:0017177,GO:0019538,GO:0031090,GO:0031974,GO:0031984,GO:0032991,GO:0033036,GO:0033365,GO:0034613,GO:0034645,GO:0036211,GO:0042742,GO:0042802,GO:0042803,GO:0042886,GO:0043170,GO:0043207,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043412,GO:0043413,GO:0044237,GO:0044238,GO:0044249,GO:0044260,GO:0044262,GO:0044267,GO:0044422,GO:0044424,GO:0044431,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0045184,GO:0046835,GO:0046907,GO:0046983,GO:0050896,GO:0051179,GO:0051234,GO:0051641,GO:0051649,GO:0051704,GO:0051707,GO:0061462,GO:0070013,GO:0070085,GO:0070727,GO:0071702,GO:0071704,GO:0071705,GO:0072594,GO:0072665,GO:0072666,GO:0098542,GO:0098588,GO:0098791,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576
Exons3
Evalue4.34e-18
EggNOG OGsKOG2397@1|root,KOG2397@2759|Eukaryota
Ec32 ortholog descriptionalpha-Glucosidase II, beta subunit
Ec32 orthologEc-01_006760.1
EC3.2.1.84
DescriptionN-glycan processing
Cds size375
COG categoryS
BRITEko00000,ko00001,ko00002,ko01000,ko04030,ko04091
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185871.4000962-CDS-P-wetherbeei_contig10480:1469..15561683185871.4000962-CDS-P-wetherbeei_contig10480:1469..1556Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10480 1470..1556 +
1683185871.417351-CDS-P-wetherbeei_contig10480:1777..19921683185871.417351-CDS-P-wetherbeei_contig10480:1777..1992Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10480 1778..1992 +
1683185871.4304528-CDS-P-wetherbeei_contig10480:2258..23311683185871.4304528-CDS-P-wetherbeei_contig10480:2258..2331Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10480 2259..2331 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig10480.1.1prot_P-wetherbeei_contig10480.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig10480 1470..2331 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig10480.1.1

>prot_P-wetherbeei_contig10480.1.1 ID=prot_P-wetherbeei_contig10480.1.1|Name=mRNA_P-wetherbeei_contig10480.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=125bp
DAEDWLRRDLGPGKILAALARRRFALDTGTFRYEVAPFVEVKQHHTLIGR
WRGFGGSTNHLLWEFDDGQHCPGAGRREARVKLVCGTEHRLVSAEEPAKC
RYGLILETPIACCDADVVEAEMAMA
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mRNA from alignment at P-wetherbeei_contig10480:1470..2331+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig10480.1.1 ID=mRNA_P-wetherbeei_contig10480.1.1|Name=mRNA_P-wetherbeei_contig10480.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=862bp|location=Sequence derived from alignment at P-wetherbeei_contig10480:1470..2331+ (Phaeothamnion wetherbeei SAG_119_79)
GACGCGGAAGACTGGCTGCGGCGGGACCTCGGCCCGGGAAAGATTCTCGC CGCCCTAGCGAGGCGTCGGTTCGCCCTCGACACGGGTGTGAGTCAAGATC AGGTGCAGGAGCTGAGTCGGCCAAGGATAGGAGCCCGTTCTCTTTGCGAG GCTGCAACGGTTAACGTTTTGCGTTTTGCTGTGTGTAGCAGGCCTGCCGT ATCGGCTGGGGGTTTCAAAGCGGCTCTCGCACCTCTCTGAGTATGTAATG TACTTGCTCCCTCGATATGTTTGCTTGTTTACGGTCGAAAAATCTGCTCT CCCCACAGACCTTCCGGTACGAGGTCGCGCCGTTCGTGGAGGTGAAGCAG CACCACACCCTCATCGGCCGGTGGCGCGGCTTCGGCGGCAGCACAAATCA CCTCCTGTGGGAATTCGACGACGGGCAACACTGTCCAGGCGCTGGGCGGC GGGAGGCACGCGTGAAGCTTGTGTGCGGGACGGAGCACCGGCTCGTTTCG GCGGAGGAGCCCGCCAAGTGCCGGTGAGCCGAATTTTTGCGTTGCATCTG CATAGATACAGCAATCGATGTCTTTCCTTTTCTTGATTGCCGTTTCTTTT GAATGCGGCTTTCCTTATTTGGGTGAAAGTCGAGCGGTTCTTCCTGTTTG GTTTAAGACGGAGGGGTCGTTTATTTTGCTCGGCTGATGCTTGCAGTGGA GGCAGGCAGCTTAGTGCTCTGCCATATCTCTTTACCAGGGTTAAATTTGT TCTAATAAACCGCAAACAACGAACGCTCCACCATGGCAGGTACGGGCTCA TCTTGGAAACGCCCATCGCGTGCTGCGATGCCGACGTCGTCGAGGCGGAG ATGGCCATGGCG
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Coding sequence (CDS) from alignment at P-wetherbeei_contig10480:1470..2331+

>mRNA_P-wetherbeei_contig10480.1.1 ID=mRNA_P-wetherbeei_contig10480.1.1|Name=mRNA_P-wetherbeei_contig10480.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=375bp|location=Sequence derived from alignment at P-wetherbeei_contig10480:1470..2331+ (Phaeothamnion wetherbeei SAG_119_79)
GACGCGGAAGACTGGCTGCGGCGGGACCTCGGCCCGGGAAAGATTCTCGC
CGCCCTAGCGAGGCGTCGGTTCGCCCTCGACACGGGTACCTTCCGGTACG
AGGTCGCGCCGTTCGTGGAGGTGAAGCAGCACCACACCCTCATCGGCCGG
TGGCGCGGCTTCGGCGGCAGCACAAATCACCTCCTGTGGGAATTCGACGA
CGGGCAACACTGTCCAGGCGCTGGGCGGCGGGAGGCACGCGTGAAGCTTG
TGTGCGGGACGGAGCACCGGCTCGTTTCGGCGGAGGAGCCCGCCAAGTGC
CGGTACGGGCTCATCTTGGAAACGCCCATCGCGTGCTGCGATGCCGACGT
CGTCGAGGCGGAGATGGCCATGGCG
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