mRNA_P-wetherbeei_contig10014.1.1 (mRNA) Phaeothamnion wetherbeei SAG_119_79

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_P-wetherbeei_contig10014.1.1
Unique NamemRNA_P-wetherbeei_contig10014.1.1
TypemRNA
OrganismPhaeothamnion wetherbeei SAG_119_79 (Phaeothamnion wetherbeei SAG_119_79)
Homology
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Match: A0A835YMB8_9STRA (CPLD51 protein required for cyt b6 assembly n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YMB8_9STRA)

HSP 1 Score: 275 bits (704), Expect = 6.490e-91
Identity = 133/181 (73.48%), Postives = 154/181 (85.08%), Query Frame = 1
Query:    1 TLGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGPAAA-GAKPLKQTAGEIVAYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQIQFPDVGAYWYAITLLSPYAGVYYWQNASRQDRASVRLETADDDKQTELIITAGEEELDRLAKTMGYQEKGKIRVKGLLED 540
            TLGLYLLSFPGLWSV+KRAA+TKY+ER+Y + GPAAA  AKP+KQ A E+VAYF ANNY+++ A + I FEG V AS+ Q FFLVFCTFLCLGTL+LVLQIQFPD G YWY +TLLSPYAGVYYW NA R+D+A VRLET DDD   E+ ITAGEEELDR+AKTMGYQ+KG IRVKG+LE+
Sbjct:   97 TLGLYLLSFPGLWSVIKRAAKTKYKERVYTVAGPAAAVAAKPVKQVAAEVVAYFQANNYKIVSAGEEIVFEGAVQASRSQGFFLVFCTFLCLGTLSLVLQIQFPDFGTYWYLLTLLSPYAGVYYWNNAQRKDKAQVRLETTDDDTTVEIRITAGEEELDRMAKTMGYQQKGMIRVKGILEE 277          
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Match: D8LHH2_ECTSI (CPLD51 protein required for cyt b6 assembly n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LHH2_ECTSI)

HSP 1 Score: 255 bits (652), Expect = 2.730e-82
Identity = 127/181 (70.17%), Postives = 148/181 (81.77%), Query Frame = 1
Query:    1 TLGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGPAAA--GAKPLKQTAGEIVAYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQIQFPDVGAYWYAITLLSPYAGVYYWQNASRQDRASVRLETADDDKQTELIITAGEEELDRLAKTMGYQEKGKIRVKGLLE 537
            TLGLY+LSFPGLWSVVKRA +TKY++  Y LPGPA+   GA+ +KQTA +I+AYF ANNY++ +A + ITFEG + ASKGQAFFLVFCT L   TLALVLQIQFP +G YW+ +TLLSPYAGVYYWQN SR D+A VRLET+DDD  TE+ IT   EELDRL+KTMGY EKG IRVKGLLE
Sbjct:  123 TLGLYVLSFPGLWSVVKRATKTKYKDATYELPGPASTEKGAESVKQTAAKIIAYFQANNYKIKEAGETITFEGAIQASKGQAFFLVFCTALSFATLALVLQIQFPAIGNYWFLLTLLSPYAGVYYWQNGSRNDKAQVRLETSDDDTLTEIYITGAAEELDRLSKTMGYMEKGMIRVKGLLE 303          
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Match: A0A7S2RI62_9STRA (Hypothetical protein (Fragment) n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2RI62_9STRA)

HSP 1 Score: 214 bits (546), Expect = 1.270e-66
Identity = 105/184 (57.07%), Postives = 133/184 (72.28%), Query Frame = 1
Query:    1 TLGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGPAAAGAKPLKQTAGEIVAYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQIQFP-----DVGAYWYAITLLSPYAGVYYWQNASRQDRASVRLETADDDKQTELIITAGEEELDRLAKTMGYQEKGKIRVKGLLE 537
            TL LYL+SFPGL+S+VKRA + K  +R Y +PGP   GA PL+  A EI AYF ANNY+VIDA D I F+G +  SK QAFFL FCT +  GTLAL+L IQ P     ++G YW+   L +PYAGVYYWQNA  +D   VRL+T+DD+++TE+I+ AG+EELD+ A  M Y EKGKIRV+G+ E
Sbjct:  101 TLALYLISFPGLFSLVKRATKVKPEQRTYEMPGPGVEGAMPLRNVAAEIFAYFQANNYKVIDAADTIVFKGVLEKSKSQAFFLSFCTLVGFGTLALILSIQVPKIGPLEIGNYWFLSCLSAPYAGVYYWQNAQNEDEVRVRLQTSDDERKTEVIVQAGKEELDKFASVMNYDEKGKIRVRGIFE 284          
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Match: A0A7S2B231_9STRA (Hypothetical protein n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2B231_9STRA)

HSP 1 Score: 205 bits (521), Expect = 2.530e-63
Identity = 103/179 (57.54%), Postives = 129/179 (72.07%), Query Frame = 1
Query:    1 TLGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGPAAAGAKPLKQTAGEIVAYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQIQFPDVGAYWYAITLLSPYAGVYYWQNASRQDRASVRLETADDDKQTELIITAGEEELDRLAKTMGYQEKGKIRVKGLLE 537
            TLGLYLLSFPGL S+VKRA +   +++ Y L GPAA  AK ++Q A EI+AYF ANNY+V DA + I F+G    S  QAFFL FCT + LG+L+LVLQIQ P VGAYW+ + L SPYAGVYYWQNA + D   VRL+  DDDK+ E+ I   +E+L+R  +T+ Y EKG IRVKG+ E
Sbjct:   87 TLGLYLLSFPGLISLVKRATKKDIKQKTYTLAGPAAEDAKSMRQVAAEIMAYFQANNYKVDDAGEVIVFKGVTQQSTSQAFFLSFCTLIGLGSLSLVLQIQVPQVGAYWWLMCLGSPYAGVYYWQNAQQDDEIRVRLQATDDDKECEVTIQGDKEQLERFQQTLQYNEKGMIRVKGIFE 265          
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Match: A0A7S3XQU3_HETAK (Hypothetical protein n=2 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XQU3_HETAK)

HSP 1 Score: 196 bits (498), Expect = 4.550e-59
Identity = 100/199 (50.25%), Postives = 139/199 (69.85%), Query Frame = 1
Query:    1 TLGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGPAA-AGAKPLKQTAGE-------------------IVAYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQIQFPDVGAYWYAITLLSPYAGVYYWQNASRQDRASVRLETADDDKQTELIITAGEEELDRLAKTMGYQEKGKIRVKGLLE 537
            TLGLY++SFPGL+S+VKR+ +    ++ Y LPGP+A  GAK +KQ A E                   +VAYF A NY +++A + I+F+G +  S  QAFFL FCTF+ LG+LALVL IQF ++G  ++ +TLL+PYAGVYYW+NA R++ A V+LE ADD++  +L+I AG+EEL+R A+TM Y EKG +RVKG+ E
Sbjct:   89 TLGLYVISFPGLYSLVKRSVKASNVKKTYELPGPSADTGAKEMKQIAAEAVREAYLPIIEAAAKQLKQVVAYFRAQNYEIVNAGETISFKGTLSKSSSQAFFLTFCTFIGLGSLALVLSIQFQEIGQNFFWMTLLAPYAGVYYWKNADREETAEVKLEIADDERTVDLVIAAGKEELERFARTMDYCEKGMVRVKGIFE 287          
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Match: B8LDY3_THAPS (Predicted protein (Fragment) n=1 Tax=Thalassiosira pseudonana TaxID=35128 RepID=B8LDY3_THAPS)

HSP 1 Score: 187 bits (476), Expect = 1.430e-57
Identity = 93/178 (52.25%), Postives = 121/178 (67.98%), Query Frame = 1
Query:    1 TLGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGPAAAGAKPLKQTAGEIVAYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQIQFPDVGAYWYAITLLSPYAGVYYWQNASRQDRASVRLETADDDKQTELIITAGEEELDRLAKTMGYQEKGKIRVKGLL 534
            TL LY  SFPGLWS +KR+ + K + + YV  G AA G K L+Q AGEI+AY  ANNY V +A + ITF G V  S  QAFFL FCT L + +LALVLQIQF DV   W+ +TLLSPYAG+YYW++  R D   V+L   DDD   E+ I   +EE++R+ +T+  QEKG ++V+G+L
Sbjct:    9 TLALYAASFPGLWSQIKRSTKAKVKRKTYVSAGEAAEGGKELRQQAGEIMAYMKANNYEVAEAGETITFRGLVARSTSQAFFLTFCTALGMASLALVLQIQFQDVDFNWFYLTLLSPYAGIYYWRSGDRVDDMKVKLAANDDDTLNEITIEGNDEEIERMWRTLDLQEKGMVKVEGIL 186          
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Match: A0A7S4A604_9STRA (Hypothetical protein n=2 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S4A604_9STRA)

HSP 1 Score: 190 bits (483), Expect = 3.570e-57
Identity = 92/184 (50.00%), Postives = 130/184 (70.65%), Query Frame = 1
Query:    1 TLGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGP-AAAGAKPLKQTAGEIVAYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQIQFPDVGAYWYAITLLSPYAGVYYWQNASR-QDRA-SVRLETADDDKQTELIITAGEEELDRLAKTMGYQEKGKIRVKGLLEDI 543
            TLGLY+LSFPGLWS +KRA +    +  Y  PG  A  G +P+++ AG+++AY  ANNY V DA + ITF G + ASK QAFFL F T +CLG+LALVLQIQFP VG YW+ + LL PYAGV+YW +A   +DR   V+L   D++ +T +++  G+E+++R+ + M  Q++G +RVKG+ ED+
Sbjct:  112 TLGLYVLSFPGLWSTIKRATKVALVQNTYEFPGENAPGGGRPMREIAGDVMAYMQANNYEVADAGETITFRGKIAASKSQAFFLSFVTLVCLGSLALVLQIQFPAVGNYWFGLCLLGPYAGVFYWGSAQENEDREFKVKLTANDEETETLMVLRGGKEDVERMEREMALQQQGMVRVKGVFEDM 295          
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Match: A0A1Y1HQD7_KLENI (Uncharacterized protein n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1HQD7_KLENI)

HSP 1 Score: 188 bits (477), Expect = 2.860e-56
Identity = 91/179 (50.84%), Postives = 126/179 (70.39%), Query Frame = 1
Query:    1 TLGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGPAAAGAKPLKQTAGEIVAYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQIQFPDVGAYWYAITLLSPYAGVYYWQNASRQDRASVRLETADDDKQTELIITAGEEELDRLAKTMGYQEKGKIRVKGLLE 537
            TLGL++LS PGLWS++KR+ ++K  ++ +V+ GPA   AKPL   A EI  +F  NNY +    + ITFEGNV  S+GQA FL FC  L L ++ALVL I  PDVG  WY +TLLSP AGVYYW+ ASR+++  V++ T+DD+  T++I+   +EE++R  + +  +EKGKI VKGLLE
Sbjct:  118 TLGLFVLSAPGLWSLIKRSTKSKIVQKTFVVDGPAKEEAKPLDSVASEIATFFKNNNYSIAGTGEVITFEGNVAPSRGQAAFLTFCAALGLLSIALVLSIAVPDVGEKWYGMTLLSPLAGVYYWRKASRKEKVKVKMVTSDDEVSTDIIVEGDDEEIERFRRELDMREKGKIYVKGLLE 296          
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Match: A0A7S2XLP2_9STRA (Hypothetical protein n=1 Tax=Attheya septentrionalis TaxID=420275 RepID=A0A7S2XLP2_9STRA)

HSP 1 Score: 185 bits (470), Expect = 6.180e-56
Identity = 91/183 (49.73%), Postives = 126/183 (68.85%), Query Frame = 1
Query:    1 TLGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGPAAAGAKPLKQTAGEIVAYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQIQFPDV----GAYWYAITLLSPYAGVYYWQNASRQDRASVRLETADDDKQTELIITAGEEELDRLAKTMGYQEKGKIRVKGLLE 537
            TLGLY LSFPGLWS +KR+   K + + +V  G A  G K L+Q AGEI+AY  ANNY V++A + ITF+G V  S  QAFFL FCT L + +LALVLQIQF +V    GA W+ + L+SPYAG+YYW++  R D   V+L   D+D + E+ I   ++E++R+ +T+  QEKG ++++GLLE
Sbjct:   56 TLGLYALSFPGLWSQIKRSTAAKTKRKTFVSSGEAVEGGKDLRQQAGEIMAYMKANNYEVVEAGETITFQGLVAKSTSQAFFLTFCTALGMASLALVLQIQFQNVELPFGANWFYLVLISPYAGIYYWRSGDRLDECKVKLVGNDEDTENEITIEGNDDEIERMWRTLELQEKGMVKIEGLLE 238          
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Match: A0A1Z5K036_FISSO (Uncharacterized protein n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5K036_FISSO)

HSP 1 Score: 185 bits (470), Expect = 1.150e-55
Identity = 96/182 (52.75%), Postives = 124/182 (68.13%), Query Frame = 1
Query:    4 LGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGPAAAGAKPLKQTAGEIVAYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQIQFPDV----GAYWYAITLLSPYAGVYYWQNASRQDRASVRLETADDDKQTELIITAGEEELDRLAKTMGYQEKGKIRVKGLLE 537
            LGLY+LSFPG+WS VKR+   K + + +V  G  AA  K L+Q AGEI+AY  +NNY V+DA + ITF G V  S GQA FLVFCT L L +LALVLQ+QF DV    G  WYAI LLSPYAGVYYW    R D  SV+L    D+ + E+++   +EEL+R+ +T+ + EKG +++ GLLE
Sbjct:   79 LGLYVLSFPGVWSQVKRSTSAKIKRKTFVTAGENAAAGKSLRQQAGEIMAYMTSNNYEVVDAGETITFRGIVQRSLGQAAFLVFCTALGLLSLALVLQVQFNDVELPFGLNWYAIALLSPYAGVYYWSAGDRVDEISVKLVANADETENEIVVQGSDEELERMWRTLEWTEKGMVKIPGLLE 260          
The following BLAST results are available for this feature:
BLAST of mRNA_P-wetherbeei_contig10014.1.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835YMB8_9STRA6.490e-9173.48CPLD51 protein required for cyt b6 assembly n=1 Ta... [more]
D8LHH2_ECTSI2.730e-8270.17CPLD51 protein required for cyt b6 assembly n=2 Ta... [more]
A0A7S2RI62_9STRA1.270e-6657.07Hypothetical protein (Fragment) n=1 Tax=Rhizochrom... [more]
A0A7S2B231_9STRA2.530e-6357.54Hypothetical protein n=1 Tax=Dictyocha speculum Ta... [more]
A0A7S3XQU3_HETAK4.550e-5950.25Hypothetical protein n=2 Tax=Heterosigma akashiwo ... [more]
B8LDY3_THAPS1.430e-5752.25Predicted protein (Fragment) n=1 Tax=Thalassiosira... [more]
A0A7S4A604_9STRA3.570e-5750.00Hypothetical protein n=2 Tax=Pelagomonas calceolat... [more]
A0A1Y1HQD7_KLENI2.860e-5650.84Uncharacterized protein n=1 Tax=Klebsormidium nite... [more]
A0A7S2XLP2_9STRA6.180e-5649.73Hypothetical protein n=1 Tax=Attheya septentrional... [more]
A0A1Z5K036_FISSO1.150e-5552.75Uncharacterized protein n=2 Tax=Fistulifera solari... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
P-wetherbeei_contig10014contigP-wetherbeei_contig10014:1880..2425 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 vs UniRef902022-09-19
Phaeothamnion wetherbeei SAG_119_79 OGS1.02022-07-08
Properties
Property NameValue
Stop1
Start0
Seed ortholog2880.D8LHH2
Preferred nameCCB1
PFAMsCCB1
Model size546
Max annot lvl2759|Eukaryota
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0008150,GO:0009507,GO:0009536,GO:0009987,GO:0010190,GO:0016043,GO:0017004,GO:0022607,GO:0034622,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043933,GO:0044085,GO:0044424,GO:0044444,GO:0044464,GO:0065003,GO:0071840
Exons1
Evalue4.47e-83
EggNOG OGs2AZTV@1|root,2QR1Q@2759|Eukaryota
Ec32 ortholog descriptionCPLD51 protein required for cyt b6 assembly
Ec32 orthologEc-11_004090.1
DescriptionCofactor assembly of complex C subunit B
Cds size546
COG categoryS
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1683185850.2306647-CDS-P-wetherbeei_contig10014:1879..24251683185850.2306647-CDS-P-wetherbeei_contig10014:1879..2425Phaeothamnion wetherbeei SAG_119_79CDSP-wetherbeei_contig10014 1880..2425 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_P-wetherbeei_contig10014.1.1prot_P-wetherbeei_contig10014.1.1Phaeothamnion wetherbeei SAG_119_79polypeptideP-wetherbeei_contig10014 1880..2425 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_P-wetherbeei_contig10014.1.1

>prot_P-wetherbeei_contig10014.1.1 ID=prot_P-wetherbeei_contig10014.1.1|Name=mRNA_P-wetherbeei_contig10014.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=polypeptide|length=182bp
TLGLYLLSFPGLWSVVKRAAQTKYRERIYVLPGPAAAGAKPLKQTAGEIV
AYFNANNYRVIDAKDAITFEGNVVASKGQAFFLVFCTFLCLGTLALVLQI
QFPDVGAYWYAITLLSPYAGVYYWQNASRQDRASVRLETADDDKQTELII
TAGEEELDRLAKTMGYQEKGKIRVKGLLEDI*
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mRNA from alignment at P-wetherbeei_contig10014:1880..2425-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_P-wetherbeei_contig10014.1.1 ID=mRNA_P-wetherbeei_contig10014.1.1|Name=mRNA_P-wetherbeei_contig10014.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=mRNA|length=546bp|location=Sequence derived from alignment at P-wetherbeei_contig10014:1880..2425- (Phaeothamnion wetherbeei SAG_119_79)
ACCCTGGGGCTCTACCTCCTCTCCTTCCCCGGGCTGTGGTCGGTGGTGAA GCGCGCCGCGCAGACCAAGTACCGGGAGCGCATCTACGTGCTGCCGGGAC CGGCGGCGGCGGGTGCCAAGCCGCTCAAGCAGACAGCGGGCGAGATCGTC GCCTACTTCAACGCCAACAACTACCGCGTCATCGACGCGAAGGACGCCAT CACCTTCGAGGGGAACGTCGTGGCCAGCAAGGGGCAGGCCTTCTTCCTCG TCTTTTGCACGTTTCTCTGCCTTGGCACGCTGGCGCTCGTCCTGCAGATT CAGTTCCCCGACGTCGGCGCTTACTGGTACGCCATCACGCTGCTGTCACC GTACGCCGGTGTCTACTACTGGCAGAACGCCAGCCGGCAGGACCGGGCAT CGGTGCGGCTTGAGACGGCCGACGACGACAAACAGACGGAATTGATCATC ACGGCGGGGGAGGAAGAGCTTGACCGGCTGGCCAAGACGATGGGCTACCA GGAGAAGGGCAAGATCCGCGTCAAGGGGCTGTTGGAGGACATTTGA
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Coding sequence (CDS) from alignment at P-wetherbeei_contig10014:1880..2425-

>mRNA_P-wetherbeei_contig10014.1.1 ID=mRNA_P-wetherbeei_contig10014.1.1|Name=mRNA_P-wetherbeei_contig10014.1.1|organism=Phaeothamnion wetherbeei SAG_119_79|type=CDS|length=546bp|location=Sequence derived from alignment at P-wetherbeei_contig10014:1880..2425- (Phaeothamnion wetherbeei SAG_119_79)
ACCCTGGGGCTCTACCTCCTCTCCTTCCCCGGGCTGTGGTCGGTGGTGAA
GCGCGCCGCGCAGACCAAGTACCGGGAGCGCATCTACGTGCTGCCGGGAC
CGGCGGCGGCGGGTGCCAAGCCGCTCAAGCAGACAGCGGGCGAGATCGTC
GCCTACTTCAACGCCAACAACTACCGCGTCATCGACGCGAAGGACGCCAT
CACCTTCGAGGGGAACGTCGTGGCCAGCAAGGGGCAGGCCTTCTTCCTCG
TCTTTTGCACGTTTCTCTGCCTTGGCACGCTGGCGCTCGTCCTGCAGATT
CAGTTCCCCGACGTCGGCGCTTACTGGTACGCCATCACGCTGCTGTCACC
GTACGCCGGTGTCTACTACTGGCAGAACGCCAGCCGGCAGGACCGGGCAT
CGGTGCGGCTTGAGACGGCCGACGACGACAAACAGACGGAATTGATCATC
ACGGCGGGGGAGGAAGAGCTTGACCGGCTGGCCAAGACGATGGGCTACCA
GGAGAAGGGCAAGATCCGCGTCAAGGGGCTGTTGGAGGACATTTGA
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