mRNA_P-canaliculata_contig99527.24062.1 (mRNA) Pelvetia canaliculata dioecious

BLAST of mRNA_P-canaliculata_contig99527.24062.1 vs. uniprot
Match: A0A518EKI0_9BACT (Uncharacterized protein n=1 Tax=Planctomycetes bacterium Poly30 TaxID=2528018 RepID=A0A518EKI0_9BACT)

HSP 1 Score: 214 bits (546), Expect = 1.400e-60
Identity = 147/368 (39.95%), Postives = 194/368 (52.72%), Query Frame = 1

Query:    1 SESAVLSLGVVGHGSTAPPLLAILTDSKEGRDLMGKERVSTRAQAFAAFGLGLLANNVDSPDLHQRVALGLMDALDSKVMREDVHVAALIALGLTPVPMTPHVPAPDLRKHRNIESAVSRSGQLAWLARRLTGPQSES----LSSAARTHGLVALGRLARDSWSGSRAKAMETLLEAATSRDADKHVRAGAWIGLGRVASGSSQPGDVAALKALAKGMLEGQPVERRFAAISLAQASSR-----LP------------ADGSPLLGYATARRALQLRLASAPTQDEPWIAMALGIQAHALHHAGVAGTSATAGALLDRLRSERGTSQIGAFAVAVALSHVGVETSPADAAKALLMEKFERTKDPAARG 1041
            SE+A LSLG++G  +   PLL +L  S+EG  L+GK  V  R  AFAAFGLGL+A+  D P L QR+A  LM+A+ +     ++  AA +A+GL PVP    VP    R   +    V+R  Q+ WL  RL   +S      L   AR HG+ ALGRL  D+    R + + TLL  ATS D+  H+R  AWIGLG V +  S   D   ++ L + +  G  +E+ FA I+LA A+SR     LP            A G    G  T RRAL  RLA       PW ++ALGIQ   L  AG +     A  L DR+R+ER  + IGA+ +A AL+H G +      A   +   F RTKDP ARG
Sbjct:  141 SETAALSLGILGEDAALQPLLDLLASSEEGGRLVGKTVVPVRTSAFAAFGLGLMAHERDEPALRQRIAAALMEAMAADHRSNEIPTAAALAIGLCPVPDKLTVPDRATRASTHAHDVVAREAQVRWLVERLDPERSRGRGDLLPRLARVHGITALGRLGSDAPPHVRERVIRTLLGVATSGDSSVHLRTAAWIGLGEVLTAGSSSLDREGMRTLMEALKTGNVLEQHFAGIALALATSREGSDPLPTEAAADDGTVDAAKGDRWAGLETGRRALLQRLARGRADSLPWFSLALGIQNWHLQRAGQSNHPVAALTLRDRVRNERSAAFIGAYGIACALAHEGSDEESRSRAGDQIHGAFRRTKDPLARG 508          

BLAST of mRNA_P-canaliculata_contig99527.24062.1 vs. uniprot
Match: A0A518F0D5_9BACT (Uncharacterized protein n=1 Tax=Planctomycetes bacterium Poly30 TaxID=2528018 RepID=A0A518F0D5_9BACT)

HSP 1 Score: 177 bits (448), Expect = 2.850e-46
Identity = 125/357 (35.01%), Postives = 184/357 (51.54%), Query Frame = 1

Query:    1 SESAVLSLGVVGHGSTAPPLLAILTDSKEGRDLMGKERVSTRAQAFAAFGLGLLANNVDSPDLHQRVALGLMDALDSKVMREDVHVAALIALGLTPVPMTPHVPAPDLRKHRNIESAVSRSGQLAWLARRLTGPQSESL--SSAARTHGLVALGRLARDSWSGSRAKAMETLLEAATSRDADKHVRAGAWIGLGRVASGSSQPGDVAALKALAKGMLEGQPVERRFAAISLAQASSRLPADGSPLLGYATARRALQLRLASAPTQDEPWIAMALGIQAHALHHA--GVAGTSATAGALLDR------LRSERGTSQIGAFAVAVALSHVGVETSPADAAKALLMEKFERTKDPAARG 1041
            SE+A L+LG+ G G++   L  +L  ++ G  L+G+  V  R +AFAAFGLG+LA +       Q VA  L+D L +     +V  AA++ALG   +P+   +P   LR H ++E+ VS +G   WL  RL   + +S   S   R   L  L + A D  +  R      L   A+ R     VR+ A + LG +A       D A+++ L + M  GQP+ERRFAA+SLAQAS+R      P    + A + L+ +       + PW A+ALG+QA  LH A  G  G  A A  LL R      L ++R  + IGA+A+  AL H G + +    A  +++E F R  +P A+G
Sbjct:  214 SETAALALGIFGEGTSFETLAELLDGTEAGAALLGRGEVPDRTRAFAAFGLGVLAFDTADVARQQEVAARLLDTLQTPSTSAEVPAAAMLALGQCKLPLAVTLPPAALRSHPDVEALVSSAGMARWLLTRLGTSERDSTITSPKERAFCLTTLAKFASDRDAALRDDVQRRLRTEASDRRRSSDVRSAALVALGELARSGKGEEDEASIRFLERMMATGQPMERRFAAMSLAQASARAGFGDDPFQSASAAAQLLRTQSRKGSCLERPWAALALGVQAFQLHQASAGAPGVPAPARVLLRRKELLAELANQRNVTTIGAYALGAALLHKGADPAARGKAGEVMLEAFRRIGEPEAKG 570          

BLAST of mRNA_P-canaliculata_contig99527.24062.1 vs. uniprot
Match: A0A523PGH1_9BACT (HEAT repeat domain-containing protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A523PGH1_9BACT)

HSP 1 Score: 130 bits (327), Expect = 5.740e-30
Identity = 109/352 (30.97%), Postives = 171/352 (48.58%), Query Frame = 1

Query:    4 ESAVLSLGVVGHGSTAPPLLAILTDSKEGRDLMGKERVSTRAQAFAAFGLGLLANNVDSPDLHQRVALGLMDALDSKVMR-EDVHVAALIALGLTPVPMTPHVPAPDLRKHRNIESAVSRSGQLAWLARRLTGPQSESLSSAARTHGLVALGRLARDSWSGSRAK----AMETLLEAATSRDADKHV-RAGAWIGLGRVASGSSQPGDVAALKALAKGMLEGQPVERRFAAISLAQASSRLPADGSPLLGYATARRALQLRLASAPTQDEPWIAMALGIQAHALHHAGVAGTSATAGALLDRLRSERGTSQIGAFAVAVALSHVGVETSPADA-AKALLMEKFERTKDPAAR 1038
            ++A ++LGV+G  S  P L  +  D+K GR+++G + V  R +AFAA+GLGL+       D+ +R+   L+  L++  M  ED+ VAA++A+GL P+ +     A    +    +   S   Q+ +L R  T  +     +  R H    L RL     +G  A+      E L+++      +  V R  A + LG V      P D     AL +    G P+++R+A ++LAQ  SR     +P  G  +AR+ L   LA A    +PW  +ALG+    LH AG   +S  + AL   L+S +  S +GA+ VA  L          DA A+AL++ K    KD +AR
Sbjct:  118 DTAAVALGVLGSESLYPLLRDVALDTKAGREIVGNDVVGERQRAFAAYGLGLIGQATADADVRRRIVADLVVLLETPGMATEDLKVAAMVAMGLVPLEVVEGTAACYCGECEVADPHTSLQAQVTYLMRYFTAKKE--FDTLVRAHTATTLARLIEAQPAGMPAELKMGVAEVLVDSLEKSAREPEVIRESAVLALGLVGDADDDPVDGWIRWALNRAAGHGDPMQKRYALVALAQVGSRPGRGDAPYAGTPSARKDLLHHLAHAKKSVKPWAGLALGVMGFHLHEAGQPLSSDASLALRTALKSSKSASDLGAYGVACGLRR--------DAKAQALMLAKISAVKDESAR 459          

BLAST of mRNA_P-canaliculata_contig99527.24062.1 vs. uniprot
Match: A0A2E5X2T0_9BACT (Uncharacterized protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2E5X2T0_9BACT)

HSP 1 Score: 109 bits (272), Expect = 9.280e-23
Identity = 105/358 (29.33%), Postives = 167/358 (46.65%), Query Frame = 1

Query:    4 ESAVLSLGVVGHGSTAPPLLAILTDSKEGRDLMGKER-VSTRAQAFAAFGLGLLANNVDSPDLHQRVALGLMDALDSKVMR--EDVHVAALIALGLTPVPMTPHV---PAPDLRKHRN-----IESAVSRSGQLAWLARRLTGPQSESLSSAARTHGLVALGRLARDSWSGSRAKAMETLLEAATSR-DADKHVRAGAWIGLGRVASGSSQPGDVAALKALAKGMLEGQPVERRFAAISLAQASSRLPADGSPLLGYATARRALQLRLASAPTQDEPWIAMALGIQAHALHHAGVAGTSATAGALLDRLRSERGTSQIGAFAVAVALSHVGVETSPADAAKALLMEKFERTKDPAARG 1041
            E+A ++LG++ +  +   L  + TD+ EGR + G E  ++ R +AFA FGLGL+ N  + P++ +R+   L   L+ +      D+ VAALI+LGL P+    H+   PA +L K  +      + A SR  QL +L   L   +   L    R H   AL RL   +    R K+ E +L     R    K +R  A + LG++        D    +AL   M +     + F+ IS+AQ +   P +G    G +  RR L   L+   ++ +PW A+A+G+        G   ++ T  AL    R E   +  GA+A+       GV       +K +L+EK ++  D  ARG
Sbjct:   33 ETAAVALGILANPESIDLLEQLATDAPEGRKVTGDESGINYRTRAFATFGLGLIGNRTEDPEVRKRLVTTLHGILEDREDHPTRDIRVAALISLGLVPIEPI-HIALKPASELAKGEDPYVLGTDPARSREDQLHYLINYLRSDRKHYLI---RAHAPSALSRLLVGAPDELRVKSAEAILINCGKRAKGQKELRQSAVLALGKIGDLDDDQIDKDIRQALYDSMGDADLQVKNFSTISVAQLAGN-PTEGDIDAGLSDIRRHLMTTLSRGKSRVKPWAALAIGVMEEKRDGLGHPMSADTLEALRTYFRKEGSPADAGAYAI-------GVGICKDQESKPILLEKLDKFTDDEARG 378          

BLAST of mRNA_P-canaliculata_contig99527.24062.1 vs. uniprot
Match: A0A2E5X634_9BACT (Uncharacterized protein n=2 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2E5X634_9BACT)

HSP 1 Score: 107 bits (267), Expect = 5.860e-22
Identity = 94/324 (29.01%), Postives = 151/324 (46.60%), Query Frame = 1

Query:    4 ESAVLSLGVVGHGSTAPPLLAILTDSKEGR---DLMGKERVSTRAQAFAAFGLGLLANNVDSPDLHQRVALGLMDALD-SKVMREDVHVAALIALGLTPVPMTPHVPAPDLRKHRNIESAVSRSGQLAWLARRLTGPQSESLSSAARTHGLVALGRLARDSWSGS----RAKAMETLLEA-ATSRDADKHVRAGAWIGLGRVASGSSQPGDVAALKALAKGMLEGQPVERRFAAISLAQASSRLPADGSPLLGYATARRALQLRLASAPTQDEPWIAMALGIQAHALHHAGVAGTSATAGALLDRLRSERGTSQIGAFAVAVAL 948
             +A LS+GV+ +   AP L ++L D+ E R   +L   E +S   +A AA+GL  +       +L + +   L+  L+  +    D+ VAA+ A+GLTP+P+     A         E   SR  Q+ WL R L   + E+  + AR H   A+ RL +D+   +    +    ET++++ A S    + V     +GLG +      P DV    +L   + +   + RRFA ISLA+  +R         G A  R+ L  +LA      +PW  +ALG+  H L   GV   +  A AL   ++  +    +GA+A+A  L
Sbjct:  125 RTAALSMGVLAYEPAAPLLRSVLLDTPEARQSLELEEGEAISADLRASAAYGLAFIGRQTSDRELRKSIVEDLVTILELEEQSTPDLKVAAVTAMGLTPLPVVDGADACYCGTCEVDEPGTSRQAQVTWLMR-LFNDKKET-PALARAHTATAMARLLQDAPDNTPKELKIGVAETMIDSLARSARETEEVHQACVLGLGLLGDADDDPVDVWIRSSLRNQLRDSDEMSRRFALISLAKVGARPGTGDGAWAGTAGVRKELTHQLARGKKHMKPWAGVALGVFGHDLMELGVKPDAGVATALRSMIKRAKKPETLGAYALAAGL 446          

BLAST of mRNA_P-canaliculata_contig99527.24062.1 vs. uniprot
Match: A0A2E6D9Z7_9BACT (Uncharacterized protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2E6D9Z7_9BACT)

HSP 1 Score: 99.4 bits (246), Expect = 2.780e-19
Identity = 92/296 (31.08%), Postives = 140/296 (47.30%), Query Frame = 1

Query:    1 SESAVLSLGVVGHGSTAPPLLAILTDSKEGRDLMGKERVSTRAQAFAAFGLGLLANNVDSPDLHQRVALGLMDALDSK-VMREDVHVAALIALGLTPVPMTPHVP------APDLRKHRNIESAVSRSGQLAWLARRLTGPQSESLSSAAR-----THGLVALGRLARDSWSGSRAKAMETLLEAATSR-DADKHVRAGAWIGLGRVASGSSQPGDVA---ALKALAKGMLEGQPVERRFAAISLAQASSRLPADG-SPLLGYATARRALQLRLASAPTQDEPWIAMALGIQAHAL 837
            +E+A ++LG++ +  +   L  + TD    R  +GK  V  R++A+A +GLGLLA    + +L Q +A  L+D L S    R D+ VAA+ ALGLTP+   P         A   RKH      +SR  QL +L      P +E  +   R     +H   A+ RL   +    R K    L+E+     D ++ +   A + LG++ +      D       + L + + EG P  RRFA ISL Q S   P +G + L G   AR+ L  +L+   T  +PW  +ALG+    L
Sbjct:   55 AETAAVALGILANDKSFEILFQLATDDPAARRFIGKTEVPVRSRAYACYGLGLLAYRTSNNELRQDIAETLVDLLASPHFSRRDIKVAAMTALGLTPLESNPDAALEGEEEAGSNRKH-----VLSRERQLDFLLD-YYNPANERANGTTRHWFVRSHAPNAMVRLLPGAAPEYREKVASLLIESVGRHSDENREIVMSATLALGQIGTAEDDGRDGLNNRIREELIRILKEGDPQSRRFAMISLGQ-SGGTPGEGENALAGGEVARKELLRQLSKGKTMLKPWAGLALGVMGRQL 343          

BLAST of mRNA_P-canaliculata_contig99527.24062.1 vs. uniprot
Match: A0A800KKL5_9BACT (HEAT repeat domain-containing protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A800KKL5_9BACT)

HSP 1 Score: 97.8 bits (242), Expect = 1.160e-18
Identity = 95/330 (28.79%), Postives = 151/330 (45.76%), Query Frame = 1

Query:    1 SESAVLSLGVVGHGSTAPPLLAILTDSKEGRDLMGKERVSTRAQAFAAFGLGLLANNVDSPDLHQRVALGLMDALDSK-VMREDVHVAALIALGLTPVPMTPHVPAP-DLRKHRNIESAVSRSGQLAWLARRLTGPQSESLSSAAR-----THGLVALGRLARDSWSGSRAKAMETLLEAATSRDAD-KHVRAGAWIGLGRVASGSSQPGDVAALKALAKGML-----EGQPVERRFAAISLAQASSRLPADGSPLLGYAT-ARRALQLRLASAPTQDEPWIAMALGIQAHALHHAGVAGTSATAGALLDRLRSERGTSQIGAFAVAVAL 948
            +E+A ++LG++ +  +   L  + TD    R  +GK  V  R +A+A +GLGLL     + +L Q +A  L+D L S    R D+ VAA+ ALGLTP+          D   + N +  +SR  QL +L      P +E  +   R      H   A+ RL   +  G +AK    L+ +      + + +   A + LG++ +      D   L    +G L     EG P  RRFA ISL Q S   P +G   L  AT AR+ L  +L+   T  +PW  +ALG+    L       + + + AL       +  ++IGA+ + + +
Sbjct:  210 AETAAVALGILANDISFEILFQLATDDPAARRFIGKTEVPIRTRAYACYGLGLLGYRTSNNELRQDIAETLVDLLTSPHFSRRDIKVAAMTALGLTPLESNVDAALEGDEEDNSNRKWVLSRERQLDFLLD-YYDPANERANGTTRHWYVRAHAPTAMARLLPGAAPGYKAKVAGLLIGSVDRYSKENREILMSATLALGQIGTAEDDGRD--GLNTQIRGELIRILKEGDPQSRRFAMISLGQ-SGGTPGEGENALAGATVARKELLRQLSKGKTMLKPWAGLALGVMGRQLLDHNETMSESASNALRAAAADCKRPAEIGAYLIGIGI 535          

BLAST of mRNA_P-canaliculata_contig99527.24062.1 vs. uniprot
Match: A0A2D6QEW2_9ARCH (Uncharacterized protein n=2 Tax=cellular organisms TaxID=131567 RepID=A0A2D6QEW2_9ARCH)

HSP 1 Score: 97.4 bits (241), Expect = 1.490e-18
Identity = 86/321 (26.79%), Postives = 143/321 (44.55%), Query Frame = 1

Query:    7 SAVLSLGVVGHGSTAPPLLAILTDSKEGRDLMGKERVSTRAQAFAAFGLGLLANNVDSPDLHQRVALGLMDALDSKVM-REDVHVAALIALGLTPVPMTPHVPAPDLRKHRNIESAVSRSGQLAWLARRLTGPQSESLSSAARTHGLVALGRLARDSWSGSRAKAMETLLEAAT------SRDADKHVRAGAWIGLGRVASGSSQPGDVAALKALAKGMLEGQPVERRFAAISLAQASSRLPADGSPLLGYATARRALQLRLASAPTQDEPWIAMALGIQAHALHHAGVAGTSATAGALLDRLRSERGTSQIGAFAVAVAL 948
            +A ++LG++ +  +   L  +  D + GR + G+ +V+   +AFA +GLGL+  +    ++ +R+   L+  L+ +     D+ VAA+ A+GL P+ +            +  +   S   Q+ +L R  T        +  R H    LGRL      G  A+  E + +         +R  D  VR  A + LG +        DV    AL + +  G P+E+RFA ISLA++ +R      P  G A+ R+ L   L+    +  PW A+A GI  + L   G         AL   L   +   ++GA A+AV L
Sbjct:  168 TAPVALGILANKDSYEALAGLARDDEMGRKIYGR-KVNDPMRAFATYGLGLIGTHTTDTNMRRRIVRDLIACLEDEGSDNSDLKVAAMTAMGLVPLEVVEDDMVCFCGTCKVADPDTSLGSQVTYLLRYFTSDGE--YDATVRAHTATTLGRLIEAQPEGMSARLKEVVTQFLVDSLGKYARQPDD-VRRSAVLALGLIGDADRDKADVWIRNALTRSVRRGDPIEKRFALISLARSGARKGTGEDPFSGTASTRKELLAALSKGKKEIRPWAALATGILGYHLRDEGGQLDPGVDNALRIALGRTKKPDEVGALALAVGL 484          

BLAST of mRNA_P-canaliculata_contig99527.24062.1 vs. uniprot
Match: A0A2D5AWB0_9BACT (Uncharacterized protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A2D5AWB0_9BACT)

HSP 1 Score: 93.6 bits (231), Expect = 2.970e-17
Identity = 104/354 (29.38%), Postives = 152/354 (42.94%), Query Frame = 1

Query:    1 SESAVLSLGVVGHGSTAPPLLAILTDSKEGRDLMGKERVSTRAQAFAAFGLGLLANNVDSPDLHQRVALGLMDALDSKVMR---EDVHVAALIALGLTPVPMTPHVPAPDLRKHRNIESAVSRSGQLAWLARRLTGPQSESLSSAARTHGLVALGRL---ARDSWSGSRAKAMETLLEAATSRDADKHVRAGAWIGLGRVASGSSQPGDVAALKALAKGMLEGQPVE-RRFAAISLAQASSRLPADGSPLLGYATARRALQLRLASAPTQDEPWIAMALGIQAHALHHAGVAGTSATAGALLDRLRSERGTSQIGAFAVAVALSHVGVETSPADAAKALLMEKFERTKDPAARG 1041
            +E+A +SLG++    +   L  +L D    R L+GK  V  R +AFAAFGLGL+ N V+   L   +   L DAL S ++     DV VA L ALGL  VP++  V A  +       +A  R  Q+ WL   L   +   L    R H   A  RL   A  SW   R      + + + +    + V+  + + LGRV   + +P + A   AL +   E + VE RRF  +++AQ  SR   +       +  R  L  RL         W A+ALG+   +   +       T   L+  L   R    +GA A+A  +           AA   L++K     D  ARG
Sbjct:  193 AETAAISLGILADRGSVEMLTNLLLDRPAARKLLGKSEVPWRTRAFAAFGLGLIGNQVEEEALRVAIVNTLCDALSSGLLELAIPDVAVACLSALGL--VPLSTAVTASGV-------AACGRVRQIDWL---LAFGRRTDLDDHVRAHLPTAAARLLIGAPASWGLKRRLGESWIEDLSGAPKVSRAVKQSSVLALGRVGFPNDEPFECAIRAALLRASRESRNVEVRRFVFVAMAQIGSRAADNDVEQKIASEIRDHLTDRLQRGVGDVRSWAALALGVLGRSPSESASQVELRTDRLLVAALGEARAPRDVGACAIACGMRQQ-------KAAGLALLKKLAEVSDDEARG 527          

BLAST of mRNA_P-canaliculata_contig99527.24062.1 vs. uniprot
Match: A0A518BKP9_9BACT (PBS lyase HEAT-like repeat protein n=1 Tax=Planctomycetes bacterium Pla133 TaxID=2528011 RepID=A0A518BKP9_9BACT)

HSP 1 Score: 91.3 bits (225), Expect = 1.790e-16
Identity = 103/355 (29.01%), Postives = 162/355 (45.63%), Query Frame = 1

Query:    1 SESAVLSLGVVGHGSTAPPLLAILTDSKEGRDLMGKERVSTRAQAFAAFGLGLLANNVDSPDLHQRVALGLMDALDSK--VMREDVHVAALIALGLTPVPMTPHVPAPDLRKHRNIESAVSRSGQLAWLARRLTGPQSESLSSAARTHGLVALGRLARD-SWSGSRAKAMETLLEAATSRDADK---HVRAGAWIGLGRVASGSSQPGDVAALKALAKGMLEGQPVERRFAAISLAQASSRLPADGSPLLGYATARRALQL--RLASAPTQDEPWIAMALGIQAHALHHAGVAGTSATAGALLDRLRSERGTSQIGAFAVAVALSHVGVETSPADAAKALLMEKFERTKDPAARG 1041
            SE+A LSLG++   ++   L A+  DSK G+D +G   V  R+QAFAA+GLGLL N    P +  RV   L D L     + R+D+ VA +I+LGLTP+   P   A D     + +   S   Q+ +L   L     E+ +   R H   A+ RL    +    R + ++ +L    +R   K    VR    + LG +        DV     L K +   Q   R+F  +S+A+ ++R P  G+  L         +L   L ++  +D+ W  MALG+  + L        ++ +  + D L   +    IGA +VA      G+   P   A+  L++    T+DP A+G
Sbjct:  208 SETAALSLGILASDNSIDLLEALARDSKLGQDAVGSTEVDPRSQAFAAYGLGLLGNRTGDPAVRSRVVAILTDLLPKAETMSRQDLAVACVISLGLTPLDPAPA--ATDADAEVSKDPTASLQAQIDFL---LEFMLDENKNYMPRAHVPTAVARLMTGLNDEVYRQRIVDAILLPVDARKGKKFVQEVRQSCALALGLIGDADDDAIDVEIRNVLLKDVQGYQSQTRKFGLMSMAKVAAR-PGSGAKDLDKNRRELTDELVKNLRNSGGEDQ-WAIMALGVYGYTLGKNEEVPPASLSLQIRDSLGKAKSPDDIGAASVAA-----GLFGDPE--AQETLLKLLGSTRDPVAQG 548