mRNA_M-pyrifera_M_contig124501.4989.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig124501.4989.1
Unique NamemRNA_M-pyrifera_M_contig124501.4989.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Match: A0A813C7N6_9DINO (PIGA protein (Fragment) n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813C7N6_9DINO)

HSP 1 Score: 200 bits (509), Expect = 6.950e-64
Identity = 84/126 (66.67%), Postives = 106/126 (84.13%), Query Frame = 1 
Query:   22 LISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSGVRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHAATSVMAHECFILARVMGYRAVYTDHSLYGFADA 399
            ++ DFFYPRLGGVEMHIWSL+QCL+ + H V+++THAYG R+G+RYMTNGLKVYY+ MTPF DQD  PT ++   LFR++L+RE IT+VHGH ATS + HEC + AR MG++AVYTDHSL+GFADA
Sbjct:    1 MVCDFFYPRLGGVEMHIWSLSQCLMRMGHKVIVITHAYGDRAGIRYMTNGLKVYYVPMTPFVDQDAFPTFYAFFPLFRKILIREGITIVHGHQATSTLTHECILHARTMGFKAVYTDHSLFGFADA 126
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BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Match: D8LMM7_ECTSI (Phosphatidylinositol N-acetylglucosaminyltransferase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LMM7_ECTSI)

HSP 1 Score: 202 bits (515), Expect = 1.230e-61
Identity = 87/132 (65.91%), Postives = 105/132 (79.55%), Query Frame = 1 
Query:    4 RRHSVCLISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSGVRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHAATSVMAHECFILARVMGYRAVYTDHSLYGFADA 399
            RRH VC++ DFFYPRLGGVEMHIWSL+QCLL   H VV++TH Y  R GVRYMTNGLKVYY+ + P  D DC+PT  +   +FRQ+L+RERIT+VHGH A+SVM+HE  + A+ MGYR  YTDHSL+GFADA
Sbjct:    2 RRHRVCMVCDFFYPRLGGVEMHIWSLSQCLLRRGHRVVVITHGYDNRKGVRYMTNGLKVYYVPLVPVVDGDCMPTACAFFPIFRQILIRERITLVHGHQASSVMSHEAILFAKTMGYRVAYTDHSLFGFADA 133
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BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Match: A0A813AD36_9DINO (PIGA protein (Fragment) n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813AD36_9DINO)

HSP 1 Score: 199 bits (507), Expect = 1.320e-61
Identity = 85/131 (64.89%), Postives = 109/131 (83.21%), Query Frame = 1 
Query:    4 RRHSVCLISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSGVRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHAATSVMAHECFILARVMGYRAVYTDHSLYGFAD 396
            ++HS+ ++SDFFYPRLGGVEMHIWSL+QCLL L H VV++T+ YG R+GVR+MTNGLKVYY  + PF DQD +PT+++ +   R +L+RERIT+VHGH ATS +AHEC   A+ MGY+AVYTDHSL+GFAD
Sbjct:   27 QKHSILMVSDFFYPRLGGVEMHIWSLSQCLLHLGHKVVVLTNQYGKRAGVRFMTNGLKVYYAPVVPFVDQDSMPTLWTFLPYLRNILIRERITIVHGHQATSTLAHECLFFAKCMGYKAVYTDHSLFGFAD 157
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BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Match: W7TP55_9STRA (Phosphatidylinositol N-acetylglucosaminyltransferase n=1 Tax=Nannochloropsis gaditana TaxID=72520 RepID=W7TP55_9STRA)

HSP 1 Score: 199 bits (506), Expect = 1.020e-59
Identity = 89/133 (66.92%), Postives = 107/133 (80.45%), Query Frame = 1 
Query:    1 RRRHSVCLISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSGVRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHAATSVMAHECFILARVMGYRAVYTDHSLYGFADA 399
            R RH+VC++ DFFYP +GGVEMHIWSLAQCLL+L H VV+VTH+Y  R GVRYM NGLKVYY  +  FYDQD  PT+F+   LFR +L+RERI++VH H ATSVM+ EC + AR MGY+ VYTDHSL+GFADA
Sbjct:    6 RPRHAVCMVCDFFYPSMGGVEMHIWSLAQCLLKLGHKVVVVTHSYDNRKGVRYMQNGLKVYYCPLLTFYDQDVWPTLFAFFPLFRHILIRERISIVHTHQATSVMSIECTLYARTMGYKVVYTDHSLFGFADA 138
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BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Match: A0A482SJN3_9ARCH (Phosphatidylinositol N-acetylglucosaminyltransferase n=1 Tax=archaeon TaxID=1906665 RepID=A0A482SJN3_9ARCH)

HSP 1 Score: 191 bits (486), Expect = 1.160e-56
Identity = 83/130 (63.85%), Postives = 101/130 (77.69%), Query Frame = 1 
Query:    7 RHSVCLISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSGVRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHAATSVMAHECFILARVMGYRAVYTDHSLYGFAD 396
            RH +C++SDFFYP +GGVE HIW LAQCL+   H V++VTHAYG R GVRYMTNGLKVYY+ +T F+DQ   PT+ S   LFR +L+RERIT+VHGH + S M +EC + AR MGYR  YTDHSL+GFAD
Sbjct:   13 RHRICMVSDFFYPNMGGVETHIWCLAQCLMLRGHKVIVVTHAYGDRQGVRYMTNGLKVYYLPLTVFFDQVIFPTLCSFFPLFRNILIRERITIVHGHQSVSSMTNECVLYARTMGYRTCYTDHSLFGFAD 142
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BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Match: A0A7S1GBW6_9STRA (Phosphatidylinositol N-acetylglucosaminyltransferase (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1GBW6_9STRA)

HSP 1 Score: 191 bits (485), Expect = 1.710e-56
Identity = 84/133 (63.16%), Postives = 106/133 (79.70%), Query Frame = 1 
Query:    1 RRRHSVCLISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSGVRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHAATSVMAHECFILARVMGYRAVYTDHSLYGFADA 399
            R ++ V ++ DFFYPRLGGVEMHIW+L+Q LL + H V+++T+ YG R GVRYMTNGLKVYY+ MTP  DQD +PT+F+   LFR +L+RERI +VHGH ATS + HEC + AR MGY+A YTDHSL+GFADA
Sbjct:   39 RVQYRVAMVCDFFYPRLGGVEMHIWALSQRLLSMGHKVIVLTNTYGDRRGVRYMTNGLKVYYMPMTPLVDQDSMPTLFACFALFRNILLRERIDIVHGHQATSTLMHECILHARTMGYKACYTDHSLFGFADA 171
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BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Match: A0A3M7RQ28_BRAPC (Phosphatidylinositol N-acetylglucosaminyltransferase n=1 Tax=Brachionus plicatilis TaxID=10195 RepID=A0A3M7RQ28_BRAPC)

HSP 1 Score: 189 bits (480), Expect = 2.330e-56
Identity = 81/134 (60.45%), Postives = 110/134 (82.09%), Query Frame = 1 
Query:    1 RRRHSVCLISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSGVRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHAATSVMAHECFILARVMGYRAVYTDHSLYGFADAT 402
            ++RHS+C++SDFFYP +GGVE HI+ L+QCLLEL+H VV++TH Y  R+GVRYMTNGLKVYYI + PFY+Q  LPTV + + + R +L+RE+IT+VHGH+A SV+A E    A+++G + VYTDHSL+GFADA+
Sbjct:    6 KKRHSICMVSDFFYPNMGGVESHIFQLSQCLLELSHKVVVITHMYNNRNGVRYMTNGLKVYYIPLVPFYNQCVLPTVLTTLPIIRDILIREQITIVHGHSAFSVLAEETLYHAKLLGLKLVYTDHSLFGFADAS 139
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BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Match: UPI0009E32BD2 (N-acetylglucosaminyl-phosphatidylinositol biosynthetic protein-like n=1 Tax=Orbicella faveolata TaxID=48498 RepID=UPI0009E32BD2)

HSP 1 Score: 179 bits (453), Expect = 3.880e-55
Identity = 75/134 (55.97%), Postives = 106/134 (79.10%), Query Frame = 1 
Query:    1 RRRHSVCLISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSGVRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHAATSVMAHECFILARVMGYRAVYTDHSLYGFADAT 402
            + +H++C++SDFF+P +GGVE HI+ L+QCL+E  H VV+VTHAYG RSGVRY+TN LKVYY+   PF++Q  LPT+++ + L R +L+RERIT+VHGH+A S + H+  + AR MG   ++TDHSL+GFADA+
Sbjct:    7 QEKHNICMVSDFFFPNMGGVESHIYQLSQCLIERGHKVVVVTHAYGERSGVRYLTNYLKVYYLPFGPFHNQCVLPTLYTTLPLMRCILIRERITIVHGHSAFSTLCHDALLHARTMGLHTLFTDHSLFGFADAS 140
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BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Match: A0A1E5WIA3_9POAL (Phosphatidylinositol N-acetylglucosaminyltransferase subunit A n=1 Tax=Dichanthelium oligosanthes TaxID=888268 RepID=A0A1E5WIA3_9POAL)

HSP 1 Score: 179 bits (453), Expect = 4.020e-54
Identity = 79/133 (59.40%), Postives = 102/133 (76.69%), Query Frame = 1 
Query:    1 RRRHSVCLISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSGVRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHAATSVMAHECFILARVMGYRAVYTDHSLYGFADA 399
            R++H + ++SDFF+P  GGVE HI+ L+QCLL+L H VV++THAYG RSGVRY+TNGLKVYY+   PF  Q+ LPT+F    + R +++RERI+VVHGH A S + HE  + AR MGY  V+TDHSLYGFADA
Sbjct:    3 RQKHRILMVSDFFFPNFGGVESHIYYLSQCLLKLGHKVVVMTHAYGNRSGVRYVTNGLKVYYVPWRPFLMQNILPTLFLTFPVVRSIIIRERISVVHGHQAFSTLCHEALMHARTMGYTVVFTDHSLYGFADA 135
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BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Match: UPI00148AC4C0 (phosphatidylinositol N-acetylglucosaminyltransferase subunit A n=1 Tax=Crassostrea gigas TaxID=29159 RepID=UPI00148AC4C0)

HSP 1 Score: 184 bits (468), Expect = 4.820e-54
Identity = 78/132 (59.09%), Postives = 103/132 (78.03%), Query Frame = 1 
Query:    7 RHSVCLISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSGVRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHAATSVMAHECFILARVMGYRAVYTDHSLYGFADAT 402
            +HS+C++SDFFYP +GGVE HI+ L+QC+LE  H VV+VTHAYG + GVRY++NGLK YY+  TPFY+Q  LPT+F+   L R + +RE+IT+VHGH+A S +AHE    A  MG R V+TDHSL+GFADA+
Sbjct:    2 KHSICMVSDFFYPNMGGVESHIYQLSQCVLERGHKVVVVTHAYGEKRGVRYLSNGLKAYYLPFTPFYNQCILPTIFTTFPLLRNIFIREQITIVHGHSAFSTLAHEAMFHASTMGLRTVFTDHSLFGFADAS 133
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig124501.4989.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A813C7N6_9DINO6.950e-6466.67PIGA protein (Fragment) n=1 Tax=Symbiodinium sp. K... [more]
D8LMM7_ECTSI1.230e-6165.91Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
A0A813AD36_9DINO1.320e-6164.89PIGA protein (Fragment) n=1 Tax=Symbiodinium sp. K... [more]
W7TP55_9STRA1.020e-5966.92Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
A0A482SJN3_9ARCH1.160e-5663.85Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
A0A7S1GBW6_9STRA1.710e-5663.16Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
A0A3M7RQ28_BRAPC2.330e-5660.45Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
UPI0009E32BD23.880e-5555.97N-acetylglucosaminyl-phosphatidylinositol biosynth... [more]
A0A1E5WIA3_9POAL4.020e-5459.40Phosphatidylinositol N-acetylglucosaminyltransfera... [more]
UPI00148AC4C04.820e-5459.09phosphatidylinositol N-acetylglucosaminyltransfera... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig124501contigM-pyrifera_M_contig124501:3..404 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score203.4
Seed ortholog evalue6.8e-50
Seed eggNOG ortholog2880.D8LMM7
Preferred namePIGA
KEGG koko:K03857
KEGG ReactionR05916
KEGG Pathwayko00563,ko01100,map00563,map01100
KEGG ModuleM00065
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0000506,GO:0000902,GO:0000904,GO:0003006,GO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0005789,GO:0005829,GO:0006464,GO:0006497,GO:0006505,GO:0006506,GO:0006629,GO:0006643,GO:0006644,GO:0006650,GO:0006661,GO:0006664,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0008194,GO:0008375,GO:0008610,GO:0008654,GO:0009058,GO:0009059,GO:0009247,GO:0009653,GO:0009826,GO:0009846,GO:0009856,GO:0009860,GO:0009893,GO:0009932,GO:0009987,GO:0010033,GO:0012505,GO:0016020,GO:0016021,GO:0016043,GO:0016049,GO:0016254,GO:0016740,GO:0016757,GO:0016758,GO:0017176,GO:0019222,GO:0019538,GO:0019637,GO:0022414,GO:0030154,GO:0030176,GO:0031224,GO:0031227,GO:0031984,GO:0032501,GO:0032502,GO:0032989,GO:0032991,GO:0034097,GO:0034645,GO:0036211,GO:0040007,GO:0042157,GO:0042158,GO:0042175,GO:0042221,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044432,GO:0044444,GO:0044446,GO:0044464,GO:0044706,GO:0045017,GO:0046467,GO:0046474,GO:0046486,GO:0046488,GO:0048468,GO:0048518,GO:0048588,GO:0048589,GO:0048856,GO:0048868,GO:0048869,GO:0050789,GO:0050896,GO:0051704,GO:0051716,GO:0060560,GO:0065007,GO:0070887,GO:0071310,GO:0071345,GO:0071704,GO:0071840,GO:0090407,GO:0098796,GO:0098827,GO:1901135,GO:1901137,GO:1901564,GO:1901566,GO:1901576,GO:1902494,GO:1903509,GO:1990234,GO:1990823,GO:1990830
EggNOG free text desc.phosphatidylinositol N-acetylglucosaminyltransferase activity
EggNOG OGsCOG0438@1,KOG1111@2759
Ec32 ortholog descriptionN-acetylglucosaminyl-phosphatidylinositol biosynthetic protein PigA, family GT4
Ec32 orthologEc-06_003950.1
EC2.4.1.198
COG Functional cat.M
CAZyGT4
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01003
Exons1
Model size402
Cds size402
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815762.5949845-CDS-M-pyrifera_M_contig124501:2..4041622815762.5949845-CDS-M-pyrifera_M_contig124501:2..404Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig124501 3..404 -
1692277557.7090347-CDS-M-pyrifera_M_contig124501:2..4041692277557.7090347-CDS-M-pyrifera_M_contig124501:2..404Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig124501 3..404 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig124501.4989.1prot_M-pyrifera_M_contig124501.4989.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig124501 3..404 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig124501.4989.1

>prot_M-pyrifera_M_contig124501.4989.1 ID=prot_M-pyrifera_M_contig124501.4989.1|Name=mRNA_M-pyrifera_M_contig124501.4989.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=134bp
RRRHSVCLISDFFYPRLGGVEMHIWSLAQCLLELNHHVVIVTHAYGARSG
VRYMTNGLKVYYISMTPFYDQDCLPTVFSGMELFRQVLVRERITVVHGHA
ATSVMAHECFILARVMGYRAVYTDHSLYGFADAT
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mRNA from alignment at M-pyrifera_M_contig124501:3..404-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig124501.4989.1 ID=mRNA_M-pyrifera_M_contig124501.4989.1|Name=mRNA_M-pyrifera_M_contig124501.4989.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=402bp|location=Sequence derived from alignment at M-pyrifera_M_contig124501:3..404- (Macrocystis pyrifera P11B4 male)
CGGCGTCGTCACTCTGTGTGTTTGATTTCTGACTTTTTCTACCCGCGTCT
GGGGGGCGTTGAGATGCACATTTGGTCTCTGGCGCAGTGTTTGCTGGAGC
TGAACCACCACGTGGTGATCGTGACGCACGCGTACGGCGCTCGGAGCGGC
GTGCGGTACATGACGAACGGGCTGAAGGTGTACTACATCTCGATGACGCC
GTTCTACGACCAGGACTGCCTGCCGACGGTGTTCAGCGGGATGGAGTTGT
TTCGGCAGGTTTTGGTGCGCGAGCGCATCACCGTGGTGCATGGCCACGCC
GCCACGTCGGTGATGGCGCACGAGTGCTTCATTCTGGCGCGCGTGATGGG
CTACAGAGCCGTGTACACAGACCACAGTCTGTACGGATTCGCAGATGCAA
CG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig124501:3..404-

>mRNA_M-pyrifera_M_contig124501.4989.1 ID=mRNA_M-pyrifera_M_contig124501.4989.1|Name=mRNA_M-pyrifera_M_contig124501.4989.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=804bp|location=Sequence derived from alignment at M-pyrifera_M_contig124501:3..404- (Macrocystis pyrifera P11B4 male)
CGGCGTCGTCACTCTGTGTGTTTGATTTCTGACTTTTTCTACCCGCGTCT
GGGGGGCGTTGAGATGCACATTTGGTCTCTGGCGCAGTGTTTGCTGGAGC
TGAACCACCACGTGGTGATCGTGACGCACGCGTACGGCGCTCGGAGCGGC
GTGCGGTACATGACGAACGGGCTGAAGGTGTACTACATCTCGATGACGCC
GTTCTACGACCAGGACTGCCTGCCGACGGTGTTCAGCGGGATGGAGTTGT
TTCGGCAGGTTTTGGTGCGCGAGCGCATCACCGTGGTGCATGGCCACGCC
GCCACGTCGGTGATGGCGCACGAGTGCTTCATTCTGGCGCGCGTGATGGG
CTACAGAGCCGTGTACACAGACCACAGTCTGTACGGATTCGCAGATGCAA
CGCGGCGTCGTCACTCTGTGTGTTTGATTTCTGACTTTTTCTACCCGCGT
CTGGGGGGCGTTGAGATGCACATTTGGTCTCTGGCGCAGTGTTTGCTGGA
GCTGAACCACCACGTGGTGATCGTGACGCACGCGTACGGCGCTCGGAGCG
GCGTGCGGTACATGACGAACGGGCTGAAGGTGTACTACATCTCGATGACG
CCGTTCTACGACCAGGACTGCCTGCCGACGGTGTTCAGCGGGATGGAGTT
GTTTCGGCAGGTTTTGGTGCGCGAGCGCATCACCGTGGTGCATGGCCACG
CCGCCACGTCGGTGATGGCGCACGAGTGCTTCATTCTGGCGCGCGTGATG
GGCTACAGAGCCGTGTACACAGACCACAGTCTGTACGGATTCGCAGATGC
AACG
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