mRNA_M-pyrifera_M_contig123138.4747.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig123138.4747.1
Unique NamemRNA_M-pyrifera_M_contig123138.4747.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Match: A0A661G557_9GAMM (Dihydroorotate dehydrogenase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A661G557_9GAMM)

HSP 1 Score: 236 bits (601), Expect = 2.460e-75
Identity = 114/147 (77.55%), Postives = 133/147 (90.48%), Query Frame = 1 
Query:    1 LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNNQGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAGASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSLHG 441
            L+TKLSPNQTDI +NARRCI+AGTDAFAVINT+MGMA+D+E R PVIGNNQGGLSGPAIKP+ALLKVHQV++  + HGIPIIGQGGIV+ASDA+EF+IAGASAVGIGTALFY+PL+  +INAGI DYLARH   +VA+L G+L LHG
Sbjct:  169 LITKLSPNQTDIADNARRCINAGTDAFAVINTLMGMAVDIESRTPVIGNNQGGLSGPAIKPVALLKVHQVHRVAREHGIPIIGQGGIVSASDALEFIIAGASAVGIGTALFYDPLLLPKINAGIADYLARHGLGSVAELTGTLQLHG 315
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BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Match: A0A3B1A0S3_9ZZZZ (Dihydroorotate dehydrogenase (NAD(+)), catalytic subunit n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B1A0S3_9ZZZZ)

HSP 1 Score: 235 bits (599), Expect = 4.790e-75
Identity = 114/145 (78.62%), Postives = 129/145 (88.97%), Query Frame = 1 
Query:    1 LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNNQGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAGASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSL 435
            L+TK+SPNQTDI  NARRCI+AGTD FAVINT+MGMAID+E R+P+IGNNQGGLSGPAIKPIALLKVHQVYQA KAH IPIIGQGGI  A+DAIEFL+AGASA+GIGTALFYEPL+C +IN GI DYL  HD  +V++LVGSL L
Sbjct:  168 LITKMSPNQTDIAENARRCIEAGTDGFAVINTLMGMAIDIETRKPIIGNNQGGLSGPAIKPIALLKVHQVYQACKAHDIPIIGQGGITTANDAIEFLMAGASAIGIGTALFYEPLVCPKINEGISDYLKTHDLSHVSELVGSLEL 312
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BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Match: B8GR92_THISH (Dihydroorotate dehydrogenase n=2 Tax=Thioalkalivibrio TaxID=106633 RepID=B8GR92_THISH)

HSP 1 Score: 234 bits (597), Expect = 1.190e-74
Identity = 114/146 (78.08%), Postives = 128/146 (87.67%), Query Frame = 1 
Query:    1 LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNNQGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAGASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSLH 438
            L+TKLSPNQTDI  NARRCI+AGTDAFAVINT+MGMAID E RE VIGNNQGGLSGPAIKPIALLKVHQVYQ  + HG+PIIGQGG+ + SDA+EFLIAGASAVG+GTALFY+PLIC +IN GIRDYL RHD  +V  L GSL+L+
Sbjct:  170 LITKLSPNQTDIAENARRCIEAGTDAFAVINTLMGMAIDAESREAVIGNNQGGLSGPAIKPIALLKVHQVYQVARPHGVPIIGQGGVTSPSDALEFLIAGASAVGVGTALFYDPLICKKINQGIRDYLDRHDLASVGQLTGSLTLN 315
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BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Match: A0A2A4MNE2_9GAMM (Dihydroorotate dehydrogenase n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A2A4MNE2_9GAMM)

HSP 1 Score: 234 bits (596), Expect = 1.590e-74
Identity = 110/147 (74.83%), Postives = 131/147 (89.12%), Query Frame = 1 
Query:    1 LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNNQGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAGASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSLHG 441
            ++TKLSPNQTDI  NARRCI+AG+DAFAVINT+MGMAID E R P+IGNNQGGLSGPAIKP+ALLKVHQVYQ  KAH IPIIGQGG+ NA+DAIEF+IAGA+AVG+GTALFY+PL+C++IN GI +YL RH+  +V DLVG+L L+G
Sbjct:  172 IITKLSPNQTDIAGNARRCIEAGSDAFAVINTLMGMAIDAEARRPIIGNNQGGLSGPAIKPVALLKVHQVYQVCKAHNIPIIGQGGVANATDAIEFMIAGATAVGVGTALFYDPLVCSKINGGIAEYLQRHNMNSVTDLVGTLRLNG 318
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BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Match: A0A523K8V2_9GAMM (Dihydroorotate dehydrogenase n=2 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A523K8V2_9GAMM)

HSP 1 Score: 234 bits (596), Expect = 1.740e-74
Identity = 111/146 (76.03%), Postives = 129/146 (88.36%), Query Frame = 1 
Query:    1 LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNNQGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAGASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSLH 438
            ++TKLSPNQTDI  NARRCI+AGTD  AVINT+MGMAID+E+R+PVIGNNQGGLSGPAIKPIALLKVHQVYQ +K H +PIIGQGGI +A DA+EF+IAGA+AVG+GTALFY+PLIC +IN GI DYL  HD  NV+DLVG+L LH
Sbjct:  168 IITKLSPNQTDIAENARRCIEAGTDGLAVINTLMGMAIDIEKRQPVIGNNQGGLSGPAIKPIALLKVHQVYQVSKHHNVPIIGQGGITSAKDALEFIIAGAAAVGVGTALFYDPLICPKINQGIVDYLDEHDIENVSDLVGTLELH 313
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BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Match: UPI000F822540 (dihydroorotate dehydrogenase n=1 Tax=Sulfuriflexus mobilis TaxID=1811807 RepID=UPI000F822540)

HSP 1 Score: 232 bits (592), Expect = 4.730e-74
Identity = 114/147 (77.55%), Postives = 126/147 (85.71%), Query Frame = 1 
Query:    1 LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNNQGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAGASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSLHG 441
            L+TKLSPNQTDI  NARRCI+AGTD  AVINT+MGMAID+E+R PVIGNNQGGLSGPAIKP+ALLKVHQVYQATK H IPIIGQGGI  A DA+EFLIAGA+ VG+GTALFYEPL C  IN GI DYL RH   +V +LVGSLSL+G
Sbjct:  163 LITKLSPNQTDIAENARRCIEAGTDGLAVINTLMGMAIDIEQRRPVIGNNQGGLSGPAIKPVALLKVHQVYQATKGHNIPIIGQGGITTAEDALEFLIAGATTVGVGTALFYEPLACQTINHGILDYLQRHGLNSVGELVGSLSLNG 309
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BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Match: A0A6M8W7G4_9PROT (Dihydroorotate dehydrogenase n=1 Tax=Proteobacteria bacterium TaxID=1977087 RepID=A0A6M8W7G4_9PROT)

HSP 1 Score: 232 bits (592), Expect = 5.670e-74
Identity = 113/146 (77.40%), Postives = 127/146 (86.99%), Query Frame = 1 
Query:    1 LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNNQGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAGASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSLH 438
            L+TKLSPNQTDI  NARRCI+AG+DAFAVINT+MGMA+DVE R PV+GNNQGGLSGPAIKP+ALLKVHQVYQ  + HGIPIIGQGGI  A DA+EFLIAGASAVGIGTALFY+PL+C +IN+GI DYL RHD   VA L GSL L+
Sbjct:  168 LITKLSPNQTDIAENARRCIEAGSDAFAVINTLMGMAVDVESRTPVMGNNQGGLSGPAIKPVALLKVHQVYQVCRNHGIPIIGQGGITTAEDALEFLIAGASAVGIGTALFYDPLVCKEINSGISDYLQRHDLHGVAQLTGSLRLN 313
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BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Match: A0A1P8UCX3_9GAMM (Dihydroorotate dehydrogenase n=1 Tax=Acidihalobacter ferrooxydans TaxID=1765967 RepID=A0A1P8UCX3_9GAMM)

HSP 1 Score: 232 bits (592), Expect = 6.800e-74
Identity = 110/147 (74.83%), Postives = 129/147 (87.76%), Query Frame = 1 
Query:    1 LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNNQGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAGASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSLHG 441
            ++TKLSPNQTDI  NARRCI+AGTD FAVINT+MGMAID+ERR P IGNNQGGLSGPAIKPIA+LK HQVYQ  KAHG+PIIGQGG+ +A DA+EFLIAGA+AVG+GTALFY+PLI A+INAG+R YL RHD  ++  + GSL+LHG
Sbjct:  169 IITKLSPNQTDIAENARRCIEAGTDGFAVINTLMGMAIDIERRVPHIGNNQGGLSGPAIKPIAVLKTHQVYQVAKAHGVPIIGQGGVASAEDALEFLIAGAAAVGVGTALFYDPLILARINAGLRAYLDRHDLDHIGQVTGSLALHG 315
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BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Match: UPI0013EC4293 (dihydroorotate dehydrogenase n=1 Tax=Thioalkalivibrio sp. XN8 TaxID=2712863 RepID=UPI0013EC4293)

HSP 1 Score: 232 bits (592), Expect = 8.140e-74
Identity = 113/147 (76.87%), Postives = 130/147 (88.44%), Query Frame = 1 
Query:    1 LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNNQGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAGASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSLHG 441
            L+TKLSPNQTDI  NARRCI+AGTDAFAVINTVMGMAID E+R PVIGN QGGLSGPAIKPIALLKVHQV+Q  + HG+PIIGQGGIV+A DA+EF+IAGA+AVG+GTALFY+PL+C +IN GIRDYL RH + +VA LVG+L L G
Sbjct:  170 LITKLSPNQTDIQENARRCIEAGTDAFAVINTVMGMAIDAEQRRPVIGNVQGGLSGPAIKPIALLKVHQVHQVAQRHGVPIIGQGGIVSARDALEFIIAGAAAVGVGTALFYDPLVCRKINEGIRDYLGRHGFGSVAQLVGTLELPG 316
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BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Match: A0A4R8IS20_9GAMM (Dihydroorotate dehydrogenase n=1 Tax=Thiohalophilus thiocyanatoxydans TaxID=381308 RepID=A0A4R8IS20_9GAMM)

HSP 1 Score: 231 bits (590), Expect = 1.320e-73
Identity = 113/146 (77.40%), Postives = 128/146 (87.67%), Query Frame = 1 
Query:    1 LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNNQGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAGASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSLH 438
            L+TKLSPNQTDI +NARRCI+AGTD FAVINT+MGMA+D+E R PVIGNNQGGLSGPAIKPIALLKVHQVYQ  + HGIPIIGQGG+ +A DAIEFLIAGASAVG+GTALFY+PLICA+INAGI  Y+ RH   NVA + GSL L+
Sbjct:  168 LITKLSPNQTDIADNARRCIEAGTDGFAVINTLMGMAVDIESRSPVIGNNQGGLSGPAIKPIALLKVHQVYQVAREHGIPIIGQGGVNSAEDAIEFLIAGASAVGVGTALFYDPLICAKINAGIVAYMNRHGLANVAQITGSLRLN 313
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig123138.4747.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A661G557_9GAMM2.460e-7577.55Dihydroorotate dehydrogenase n=1 Tax=Gammaproteoba... [more]
A0A3B1A0S3_9ZZZZ4.790e-7578.62Dihydroorotate dehydrogenase (NAD(+)), catalytic s... [more]
B8GR92_THISH1.190e-7478.08Dihydroorotate dehydrogenase n=2 Tax=Thioalkalivib... [more]
A0A2A4MNE2_9GAMM1.590e-7474.83Dihydroorotate dehydrogenase n=1 Tax=Gammaproteoba... [more]
A0A523K8V2_9GAMM1.740e-7476.03Dihydroorotate dehydrogenase n=2 Tax=Gammaproteoba... [more]
UPI000F8225404.730e-7477.55dihydroorotate dehydrogenase n=1 Tax=Sulfuriflexus... [more]
A0A6M8W7G4_9PROT5.670e-7477.40Dihydroorotate dehydrogenase n=1 Tax=Proteobacteri... [more]
A0A1P8UCX3_9GAMM6.800e-7474.83Dihydroorotate dehydrogenase n=1 Tax=Acidihalobact... [more]
UPI0013EC42938.140e-7476.87dihydroorotate dehydrogenase n=1 Tax=Thioalkalivib... [more]
A0A4R8IS20_9GAMM1.320e-7377.40Dihydroorotate dehydrogenase n=1 Tax=Thiohalophilu... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig123138contigM-pyrifera_M_contig123138:2..448 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score236.5
Seed ortholog evalue8e-60
Seed eggNOG ortholog396588.Tgr7_3279
Preferred namepyrD
KEGG rclassRC00051
KEGG koko:K00226,ko:K02823,ko:K17828
KEGG ReactionR01867,R01869
KEGG Pathwayko00240,ko01100,map00240,map01100
KEGG ModuleM00051
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004152,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006139,GO:0006206,GO:0006207,GO:0006220,GO:0006221,GO:0006725,GO:0006753,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0009058,GO:0009112,GO:0009117,GO:0009165,GO:0009218,GO:0009220,GO:0009259,GO:0009260,GO:0009987,GO:0016491,GO:0016627,GO:0016635,GO:0018130,GO:0019438,GO:0019637,GO:0019693,GO:0019856,GO:0034641,GO:0034654,GO:0044237,GO:0044238,GO:0044249,GO:0044271,GO:0044281,GO:0044424,GO:0044464,GO:0046112,GO:0046390,GO:0046483,GO:0055086,GO:0055114,GO:0071704,GO:0072527,GO:0072528,GO:0090407,GO:1901135,GO:1901137,GO:1901293,GO:1901360,GO:1901362,GO:1901564,GO:1901566,GO:1901576
EggNOG free text desc.Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily
EggNOG OGs1MU7C@1224,1T02D@1236,1WW5T@135613,COG0167@1,COG0167@2
EC1.3.1.14,1.3.98.1
COG Functional cat.F
BiGG ReactioniYO844.BSU15540
Best tax levelChromatiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size447
Cds size447
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815750.2512002-CDS-M-pyrifera_M_contig123138:1..4481622815750.2512002-CDS-M-pyrifera_M_contig123138:1..448Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig123138 2..448 +
1692277551.7813535-CDS-M-pyrifera_M_contig123138:1..4481692277551.7813535-CDS-M-pyrifera_M_contig123138:1..448Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig123138 2..448 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig123138.4747.1prot_M-pyrifera_M_contig123138.4747.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig123138 2..448 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig123138.4747.1

>prot_M-pyrifera_M_contig123138.4747.1 ID=prot_M-pyrifera_M_contig123138.4747.1|Name=mRNA_M-pyrifera_M_contig123138.4747.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=149bp
LVTKLSPNQTDIVNNARRCIDAGTDAFAVINTVMGMAIDVERREPVIGNN
QGGLSGPAIKPIALLKVHQVYQATKAHGIPIIGQGGIVNASDAIEFLIAG
ASAVGIGTALFYEPLICAQINAGIRDYLARHDYPNVADLVGSLSLHGL*
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mRNA from alignment at M-pyrifera_M_contig123138:2..448+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig123138.4747.1 ID=mRNA_M-pyrifera_M_contig123138.4747.1|Name=mRNA_M-pyrifera_M_contig123138.4747.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=447bp|location=Sequence derived from alignment at M-pyrifera_M_contig123138:2..448+ (Macrocystis pyrifera P11B4 male)
CTCGTCACGAAGCTGTCGCCGAACCAGACCGACATCGTCAACAACGCGCG
CCGCTGTATCGACGCCGGCACGGACGCGTTCGCGGTGATCAACACGGTGA
TGGGTATGGCAATCGACGTCGAGCGGCGTGAACCGGTAATCGGCAATAAC
CAGGGTGGCTTGTCCGGACCGGCGATCAAGCCGATCGCGTTACTTAAAGT
GCATCAGGTTTACCAGGCGACGAAAGCGCACGGAATTCCGATTATCGGCC
AGGGCGGGATCGTCAATGCGAGCGATGCGATTGAATTCCTGATCGCGGGC
GCGTCGGCAGTGGGAATTGGCACGGCGCTGTTCTACGAACCGCTGATCTG
CGCGCAAATCAACGCTGGCATCCGCGACTATCTCGCGCGTCACGATTACC
CGAACGTGGCTGATCTCGTCGGTTCGCTCAGCCTGCACGGCCTGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig123138:2..448+

>mRNA_M-pyrifera_M_contig123138.4747.1 ID=mRNA_M-pyrifera_M_contig123138.4747.1|Name=mRNA_M-pyrifera_M_contig123138.4747.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=894bp|location=Sequence derived from alignment at M-pyrifera_M_contig123138:2..448+ (Macrocystis pyrifera P11B4 male)
CTCGTCACGAAGCTGTCGCCGAACCAGACCGACATCGTCAACAACGCGCG
CCGCTGTATCGACGCCGGCACGGACGCGTTCGCGGTGATCAACACGGTGA
TGGGTATGGCAATCGACGTCGAGCGGCGTGAACCGGTAATCGGCAATAAC
CAGGGTGGCTTGTCCGGACCGGCGATCAAGCCGATCGCGTTACTTAAAGT
GCATCAGGTTTACCAGGCGACGAAAGCGCACGGAATTCCGATTATCGGCC
AGGGCGGGATCGTCAATGCGAGCGATGCGATTGAATTCCTGATCGCGGGC
GCGTCGGCAGTGGGAATTGGCACGGCGCTGTTCTACGAACCGCTGATCTG
CGCGCAAATCAACGCTGGCATCCGCGACTATCTCGCGCGTCACGATTACC
CGAACGTGGCTGATCTCGTCGGTTCGCTCAGCCTGCACGGCCTGTAACTC
GTCACGAAGCTGTCGCCGAACCAGACCGACATCGTCAACAACGCGCGCCG
CTGTATCGACGCCGGCACGGACGCGTTCGCGGTGATCAACACGGTGATGG
GTATGGCAATCGACGTCGAGCGGCGTGAACCGGTAATCGGCAATAACCAG
GGTGGCTTGTCCGGACCGGCGATCAAGCCGATCGCGTTACTTAAAGTGCA
TCAGGTTTACCAGGCGACGAAAGCGCACGGAATTCCGATTATCGGCCAGG
GCGGGATCGTCAATGCGAGCGATGCGATTGAATTCCTGATCGCGGGCGCG
TCGGCAGTGGGAATTGGCACGGCGCTGTTCTACGAACCGCTGATCTGCGC
GCAAATCAACGCTGGCATCCGCGACTATCTCGCGCGTCACGATTACCCGA
ACGTGGCTGATCTCGTCGGTTCGCTCAGCCTGCACGGCCTGTAA
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