mRNA_M-pyrifera_M_contig122017.4534.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig122017.4534.1
Unique NamemRNA_M-pyrifera_M_contig122017.4534.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Match: A0A7S1C4D7_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1C4D7_9STRA)

HSP 1 Score: 195 bits (495), Expect = 1.970e-57
Identity = 96/132 (72.73%), Postives = 113/132 (85.61%), Query Frame = 1 
Query:   10 DEEDVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLETTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPPALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK 405
            +E DV +   LPDTA+LSE+LR+CLQE E LP DFT+LF+D  F  +T L+PEAV LYD L VKHEPLS+IPPQFETPLPPL+PAVFPPT +E PPPALDQFDLDEHFASERLRLAQL NKC+DDD+D+F++
Sbjct:  319 EEPDVSDYVYLPDTAALSERLRSCLQESEDLPKDFTQLFDDTLFKFDTDLIPEAVKLYDALNVKHEPLSLIPPQFETPLPPLQPAVFPPTLRELPPPALDQFDLDEHFASERLRLAQLTNKCTDDDLDYFVR 450
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BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Match: A0A2D4CGB3_PYTIN (Intraflagellar Transport Protein 52 n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CGB3_PYTIN)

HSP 1 Score: 191 bits (484), Expect = 4.270e-56
Identity = 92/129 (71.32%), Postives = 111/129 (86.05%), Query Frame = 1 
Query:   19 DVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLETTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPPALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK 405
            D+ E   LPDT +LSE+LR+C+QE E LP DFTRLF+   F  +T+L+PEAV+LY +LGVKHEPL++IPPQFE PLPPLKPAVFPPT +EPPPPALDQFDLDEHFASE LRLAQL NKCSDDD+++++K
Sbjct:  273 DLSEYNRLPDTQALSERLRSCMQESEELPKDFTRLFDSALFKFDTSLIPEAVALYKELGVKHEPLTLIPPQFELPLPPLKPAVFPPTLREPPPPALDQFDLDEHFASETLRLAQLTNKCSDDDLEYYVK 401
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BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Match: A0A8K1FT64_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1FT64_PYTOL)

HSP 1 Score: 190 bits (482), Expect = 1.120e-55
Identity = 91/129 (70.54%), Postives = 111/129 (86.05%), Query Frame = 1 
Query:   19 DVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLETTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPPALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK 405
            D+ E   LPDT +LSE+LR+C+QE E LP DFTRLF+   F  +T+L+PEAV+LY +LGVKHEPL++IPPQFE PLPPLKPA+FPPT +EPPPPALDQFDLDEHFASE LRLAQL NKCSDDD+++++K
Sbjct:  289 DLAEYNRLPDTQALSERLRSCMQESEELPKDFTRLFDSSLFKFDTSLIPEAVALYKELGVKHEPLTLIPPQFELPLPPLKPAMFPPTLREPPPPALDQFDLDEHFASESLRLAQLTNKCSDDDLEYYVK 417
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BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Match: A0A5D6YIU5_9STRA (Uncharacterized protein n=1 Tax=Pythium brassicum TaxID=1485010 RepID=A0A5D6YIU5_9STRA)

HSP 1 Score: 187 bits (476), Expect = 6.800e-55
Identity = 91/129 (70.54%), Postives = 111/129 (86.05%), Query Frame = 1 
Query:   19 DVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLETTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPPALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK 405
            D+ + T LPDT +LSE+LR+C+QE E LP DFTRLF+D  F  +T L+PEAVSL+ +LGVK+EPL++IPPQFE PLPPLK AVFPPT +EPPPPALDQFDLDEHFASE LRLAQL NKCSDDD+++++K
Sbjct:  298 DLSDYTRLPDTQALSERLRSCMQESEELPKDFTRLFDDSLFKFDTALIPEAVSLHKELGVKYEPLTLIPPQFECPLPPLKSAVFPPTLREPPPPALDQFDLDEHFASESLRLAQLTNKCSDDDLEYYVK 426
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BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Match: K3WSL2_GLOUD (Uncharacterized protein n=1 Tax=Globisporangium ultimum (strain ATCC 200006 / CBS 805.95 / DAOM BR144) TaxID=431595 RepID=K3WSL2_GLOUD)

HSP 1 Score: 187 bits (474), Expect = 1.850e-54
Identity = 91/129 (70.54%), Postives = 111/129 (86.05%), Query Frame = 1 
Query:   19 DVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLETTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPPALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK 405
            D+ E   LPDT +LSE+LR+C+QE E LP DFTRLF+D  F  +T+L+PEAVSL+ +LGVK+EPL++IPPQFE PLPPLK AVFPPT +EPPPPALDQFDLDEHFASE LRLAQL NKCSDDD+++++K
Sbjct:  290 DLSEYNRLPDTQALSERLRSCMQESEELPKDFTRLFDDSLFKFDTSLIPEAVSLHRELGVKYEPLTLIPPQFECPLPPLKSAVFPPTLREPPPPALDQFDLDEHFASESLRLAQLTNKCSDDDLEYYVK 418
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BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Match: A0A485KGV6_9STRA (Aste57867_5219 protein n=8 Tax=Aphanomyces TaxID=100860 RepID=A0A485KGV6_9STRA)

HSP 1 Score: 186 bits (471), Expect = 4.380e-54
Identity = 89/129 (68.99%), Postives = 111/129 (86.05%), Query Frame = 1 
Query:   19 DVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLETTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPPALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK 405
            D+ +   LPDT +LSE+LR+C+QE E LP DFT+LF++  F  +T+L+P+AV L+ +LGVKHEPLS+IPPQFETPLPPLKPAVFPP  +EPPPPALDQFDLDEHFASE LRLAQL NKCSDDD+++++K
Sbjct:  286 DLSDYNRLPDTQALSERLRSCMQEGEELPKDFTKLFDNTLFKFDTSLIPDAVLLFKELGVKHEPLSLIPPQFETPLPPLKPAVFPPILREPPPPALDQFDLDEHFASESLRLAQLTNKCSDDDLEYYVK 414
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BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Match: A0A6A3U2F0_9STRA (Intraflagellar transport protein 52 n=3 Tax=Peronosporaceae TaxID=4777 RepID=A0A6A3U2F0_9STRA)

HSP 1 Score: 185 bits (470), Expect = 5.040e-54
Identity = 89/129 (68.99%), Postives = 110/129 (85.27%), Query Frame = 1 
Query:   19 DVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLETTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPPALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK 405
            D+ E   LPDT +LS++LR+C+QE E LP DFTRLF+D  F  +T+L+PEAV+LY +LGVKHEPL++IPPQFE PLPPLK AVFPP  +EPPPPALDQFDLDEHFASE LRLAQL NKCSDDD++++++
Sbjct:  251 DLNEYVRLPDTQALSDRLRSCMQESEELPKDFTRLFDDSLFKFDTSLIPEAVNLYRELGVKHEPLTLIPPQFECPLPPLKSAVFPPALREPPPPALDQFDLDEHFASESLRLAQLTNKCSDDDLEYYVR 379
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BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Match: H3GGX9_PHYRM (Uncharacterized protein n=21 Tax=Phytophthora TaxID=4783 RepID=H3GGX9_PHYRM)

HSP 1 Score: 185 bits (469), Expect = 1.450e-53
Identity = 89/129 (68.99%), Postives = 110/129 (85.27%), Query Frame = 1 
Query:   19 DVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLETTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPPALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK 405
            D+ E   LPDT +LS++LR+C+QE E LP DFTRLF+D  F  +T+L+PEAV+LY +LGVKHEPL++IPPQFE PLPPLK AVFPP  +EPPPPALDQFDLDEHFASE LRLAQL NKCSDDD++++++
Sbjct:  288 DLNEYVRLPDTQALSDRLRSCMQESEELPKDFTRLFDDALFKFDTSLIPEAVNLYRELGVKHEPLTLIPPQFECPLPPLKSAVFPPALREPPPPALDQFDLDEHFASESLRLAQLTNKCSDDDLEYYVR 416
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BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Match: A0A812NZL0_9DINO (IFT52 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812NZL0_9DINO)

HSP 1 Score: 184 bits (466), Expect = 3.080e-53
Identity = 90/131 (68.70%), Postives = 107/131 (81.68%), Query Frame = 1 
Query:   13 EEDVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLETTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPPALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK 405
            E DV + T LPDT SL+E+LR+CLQ+ +PLPTDFTRLF+D  F      VPEAV LY  LGVKHEPL++IPPQF+TPLPPL PAVFPP   EPPPPAL+ FDLDEHFASER+RLAQL NKC+D D+DF+++
Sbjct:  272 EGDVADYTFLPDTTSLAERLRSCLQDHDPLPTDFTRLFDDGLFSFHVDNVPEAVKLYQDLGVKHEPLTVIPPQFDTPLPPLAPAVFPPAMHEPPPPALELFDLDEHFASERIRLAQLTNKCTDGDLDFYLR 402
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BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Match: A0A1V9ZUQ0_9STRA (Intraflagellar Transport Protein 52 n=3 Tax=Saprolegniaceae TaxID=4764 RepID=A0A1V9ZUQ0_9STRA)

HSP 1 Score: 183 bits (465), Expect = 3.570e-53
Identity = 91/135 (67.41%), Postives = 112/135 (82.96%), Query Frame = 1 
Query:    1 GEEDEEDVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLETTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPPALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK 405
            G+ D  D F    LPDT +LSE+LR+C+QE E LP DFT+LF++  F  +T+L+PEAV+L+  LGVKHEPL++IPPQFE PLPPLKPAVFPP  +EPPPPALDQFDLDEHFASE LRLAQL NKCSDDD+++++K
Sbjct:  285 GDPDLSDYFR---LPDTQALSERLRSCMQEGEELPKDFTKLFDNSLFKFDTSLIPEAVALFKDLGVKHEPLTLIPPQFECPLPPLKPAVFPPILREPPPPALDQFDLDEHFASESLRLAQLTNKCSDDDLEYYVK 416
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig122017.4534.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S1C4D7_9STRA1.970e-5772.73Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]
A0A2D4CGB3_PYTIN4.270e-5671.32Intraflagellar Transport Protein 52 n=1 Tax=Pythiu... [more]
A0A8K1FT64_PYTOL1.120e-5570.54Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A5D6YIU5_9STRA6.800e-5570.54Uncharacterized protein n=1 Tax=Pythium brassicum ... [more]
K3WSL2_GLOUD1.850e-5470.54Uncharacterized protein n=1 Tax=Globisporangium ul... [more]
A0A485KGV6_9STRA4.380e-5468.99Aste57867_5219 protein n=8 Tax=Aphanomyces TaxID=1... [more]
A0A6A3U2F0_9STRA5.040e-5468.99Intraflagellar transport protein 52 n=3 Tax=Perono... [more]
H3GGX9_PHYRM1.450e-5368.99Uncharacterized protein n=21 Tax=Phytophthora TaxI... [more]
A0A812NZL0_9DINO3.080e-5368.70IFT52 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=2... [more]
A0A1V9ZUQ0_9STRA3.570e-5367.41Intraflagellar Transport Protein 52 n=3 Tax=Saprol... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig122017contigM-pyrifera_M_contig122017:2..406 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score190.7
Seed ortholog evalue4.6e-46
Seed eggNOG ortholog36331.EPrPI00000021512
Preferred nameIFT52
KEGG koko:K19681
Hectar predicted targeting categoryother localisation
GOsGO:0001838,GO:0001841,GO:0001947,GO:0002009,GO:0003002,GO:0003007,GO:0003008,GO:0003143,GO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005813,GO:0005814,GO:0005815,GO:0005856,GO:0005871,GO:0005875,GO:0005929,GO:0006810,GO:0006928,GO:0006935,GO:0006950,GO:0006970,GO:0006996,GO:0007017,GO:0007018,GO:0007154,GO:0007165,GO:0007166,GO:0007224,GO:0007275,GO:0007368,GO:0007389,GO:0007399,GO:0007507,GO:0007600,GO:0008022,GO:0008150,GO:0008285,GO:0009605,GO:0009628,GO:0009653,GO:0009790,GO:0009792,GO:0009799,GO:0009855,GO:0009887,GO:0009888,GO:0009953,GO:0009987,GO:0010468,GO:0010970,GO:0015630,GO:0016043,GO:0016331,GO:0019222,GO:0021915,GO:0022607,GO:0023052,GO:0030030,GO:0030031,GO:0030162,GO:0030326,GO:0030425,GO:0030705,GO:0030990,GO:0030992,GO:0031503,GO:0031514,GO:0032391,GO:0032501,GO:0032502,GO:0032991,GO:0035050,GO:0035107,GO:0035108,GO:0035113,GO:0035148,GO:0035239,GO:0035295,GO:0035720,GO:0035735,GO:0035869,GO:0036064,GO:0036477,GO:0040011,GO:0042073,GO:0042127,GO:0042221,GO:0042330,GO:0042733,GO:0042995,GO:0043005,GO:0043009,GO:0043025,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0044085,GO:0044292,GO:0044297,GO:0044422,GO:0044424,GO:0044430,GO:0044441,GO:0044444,GO:0044446,GO:0044450,GO:0044463,GO:0044464,GO:0044782,GO:0046907,GO:0048513,GO:0048519,GO:0048523,GO:0048562,GO:0048568,GO:0048598,GO:0048646,GO:0048729,GO:0048731,GO:0048736,GO:0048856,GO:0050678,GO:0050680,GO:0050789,GO:0050794,GO:0050877,GO:0050896,GO:0050954,GO:0051171,GO:0051179,GO:0051234,GO:0051246,GO:0051641,GO:0051649,GO:0051716,GO:0060173,GO:0060255,GO:0060271,GO:0060429,GO:0060562,GO:0061371,GO:0065007,GO:0070613,GO:0070887,GO:0070925,GO:0071840,GO:0072175,GO:0072359,GO:0080090,GO:0097447,GO:0097458,GO:0097542,GO:0097546,GO:0097730,GO:0097731,GO:0097733,GO:0099111,GO:0120025,GO:0120031,GO:0120036,GO:0120038,GO:1902074,GO:1902075,GO:1903317,GO:1905515
EggNOG free text desc.Intraflagellar Transport Protein 52. Source PGD
EggNOG OGs1MEX5@121069,KOG3861@1,KOG3861@2759
COG Functional cat.W
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko03036
Exons1
Model size405
Cds size405
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815740.270228-CDS-M-pyrifera_M_contig122017:1..4061622815740.270228-CDS-M-pyrifera_M_contig122017:1..406Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig122017 2..406 -
1692277547.2460148-CDS-M-pyrifera_M_contig122017:1..4061692277547.2460148-CDS-M-pyrifera_M_contig122017:1..406Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig122017 2..406 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig122017.4534.1prot_M-pyrifera_M_contig122017.4534.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig122017 2..406 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig122017.4534.1

>prot_M-pyrifera_M_contig122017.4534.1 ID=prot_M-pyrifera_M_contig122017.4534.1|Name=mRNA_M-pyrifera_M_contig122017.4534.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=135bp
GEEDEEDVFERTLLPDTASLSEKLRACLQEPEPLPTDFTRLFEDKPFGLE
TTLVPEAVSLYDKLGVKHEPLSMIPPQFETPLPPLKPAVFPPTFKEPPPP
ALDQFDLDEHFASERLRLAQLANKCSDDDIDFFIK
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mRNA from alignment at M-pyrifera_M_contig122017:2..406-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig122017.4534.1 ID=mRNA_M-pyrifera_M_contig122017.4534.1|Name=mRNA_M-pyrifera_M_contig122017.4534.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=405bp|location=Sequence derived from alignment at M-pyrifera_M_contig122017:2..406- (Macrocystis pyrifera P11B4 male)
GGGGAGGAAGACGAAGAAGATGTGTTTGAACGAACACTCCTCCCAGACAC
TGCTTCCCTCAGTGAAAAACTCAGAGCTTGTTTGCAAGAACCAGAACCTC
TTCCCACCGATTTTACTCGTCTCTTTGAAGACAAACCCTTTGGCCTCGAA
ACCACTCTAGTTCCAGAAGCTGTGTCTCTCTACGACAAGTTGGGGGTGAA
ACACGAACCTCTCTCCATGATCCCCCCGCAGTTTGAAACACCTCTCCCTC
CTCTAAAACCGGCAGTTTTCCCCCCCACTTTCAAAGAACCCCCTCCTCCA
GCGTTGGACCAGTTTGATCTCGATGAACACTTTGCTTCCGAACGGCTTCG
ACTCGCTCAATTAGCCAACAAATGCTCCGACGATGACATTGACTTCTTCA
TCAAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig122017:2..406-

>mRNA_M-pyrifera_M_contig122017.4534.1 ID=mRNA_M-pyrifera_M_contig122017.4534.1|Name=mRNA_M-pyrifera_M_contig122017.4534.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=810bp|location=Sequence derived from alignment at M-pyrifera_M_contig122017:2..406- (Macrocystis pyrifera P11B4 male)
GGGGAGGAAGACGAAGAAGATGTGTTTGAACGAACACTCCTCCCAGACAC
TGCTTCCCTCAGTGAAAAACTCAGAGCTTGTTTGCAAGAACCAGAACCTC
TTCCCACCGATTTTACTCGTCTCTTTGAAGACAAACCCTTTGGCCTCGAA
ACCACTCTAGTTCCAGAAGCTGTGTCTCTCTACGACAAGTTGGGGGTGAA
ACACGAACCTCTCTCCATGATCCCCCCGCAGTTTGAAACACCTCTCCCTC
CTCTAAAACCGGCAGTTTTCCCCCCCACTTTCAAAGAACCCCCTCCTCCA
GCGTTGGACCAGTTTGATCTCGATGAACACTTTGCTTCCGAACGGCTTCG
ACTCGCTCAATTAGCCAACAAATGCTCCGACGATGACATTGACTTCTTCA
TCAAGGGGGAGGAAGACGAAGAAGATGTGTTTGAACGAACACTCCTCCCA
GACACTGCTTCCCTCAGTGAAAAACTCAGAGCTTGTTTGCAAGAACCAGA
ACCTCTTCCCACCGATTTTACTCGTCTCTTTGAAGACAAACCCTTTGGCC
TCGAAACCACTCTAGTTCCAGAAGCTGTGTCTCTCTACGACAAGTTGGGG
GTGAAACACGAACCTCTCTCCATGATCCCCCCGCAGTTTGAAACACCTCT
CCCTCCTCTAAAACCGGCAGTTTTCCCCCCCACTTTCAAAGAACCCCCTC
CTCCAGCGTTGGACCAGTTTGATCTCGATGAACACTTTGCTTCCGAACGG
CTTCGACTCGCTCAATTAGCCAACAAATGCTCCGACGATGACATTGACTT
CTTCATCAAG
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