mRNA_M-pyrifera_M_contig120463.4225.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig120463.4225.1
Unique NamemRNA_M-pyrifera_M_contig120463.4225.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Match: A0A2E7KMM7_9PLAN (CHAD domain-containing protein n=6 Tax=Gimesia TaxID=1649453 RepID=A0A2E7KMM7_9PLAN)

HSP 1 Score: 343 bits (880), Expect = 2.560e-117
Identity = 172/179 (96.09%), Postives = 176/179 (98.32%), Query Frame = 1 
Query:    1 WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPAYVDLFAAFECCFRERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIVAAQLEELDQLNDYLGDDHDLAVMTSQLTSEPERFGAT 537
            WYRDAGR+LSRVRDAESMLESLAKLKQRFDDPAYVDLF AFE CF+ERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGK+DKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPI+AAQLEELDQLNDYLGDDHDLAVMTSQLT EPERFGAT
Sbjct:   72 WYRDAGRRLSRVRDAESMLESLAKLKQRFDDPAYVDLFTAFERCFQERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKADKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIMAAQLEELDQLNDYLGDDHDLAVMTSQLTGEPERFGAT 250
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BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Match: A0A518A1G2_9PLAN (CHAD domain protein n=3 Tax=Gimesia panareensis TaxID=2527978 RepID=A0A518A1G2_9PLAN)

HSP 1 Score: 294 bits (753), Expect = 5.200e-98
Identity = 147/178 (82.58%), Postives = 161/178 (90.45%), Query Frame = 1 
Query:    1 WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPAYVDLFAAFECCFRERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIVAAQLEELDQLNDYLGDDHDLAVMTSQLTSEPERFGA 534
            WYRDAGR+LSRVRDAESMLESLAKLK RFDDPAY +LFAAFE CF+ RKQQLVEEWIDL+QELQ LC+ELQTA +QI+ WKIKG  DKIL+ GLQ+NYRRGAEALA L+ QP DELFHECRKRSKYL+YH+RLLK VWEPI++AQL  LDQLNDYLGDDHDLAVMT QLTSEPERFGA
Sbjct:   72 WYRDAGRRLSRVRDAESMLESLAKLKVRFDDPAYTELFAAFEKCFQARKQQLVEEWIDLEQELQTLCEELQTAQKQIENWKIKGNPDKILREGLQQNYRRGAEALATLHEQPSDELFHECRKRSKYLLYHMRLLKDVWEPILSAQLSVLDQLNDYLGDDHDLAVMTCQLTSEPERFGA 249
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BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Match: A0A355G5K3_9PLAN (CHAD domain-containing protein n=2 Tax=Planctomycetaceae TaxID=126 RepID=A0A355G5K3_9PLAN)

HSP 1 Score: 233 bits (595), Expect = 4.520e-74
Identity = 116/179 (64.80%), Postives = 143/179 (79.89%), Query Frame = 1 
Query:    1 WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPAYVDLFAAFECCFRERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIVAAQLEELDQLNDYLGDDHDLAVMTSQLTSEPERFGAT 537
            WYRD GR+LSR+RDAES+LESL  L++RF D AY +LFA FE  F+ERKQ++VEEW DLDQEL+QL  ELQ A Q+++ WKIKG + KILK  L+R Y RGA ALA+L+  P+DELFHECRKRSKY +YH+RLL  VW  I++A+  ELD+LNDYLGDDHDLAVMT  +T +PE FG +
Sbjct:   72 WYRDVGRRLSRIRDAESLLESLQALQERFPDSAYTELFAEFEKRFQERKQKVVEEWTDLDQELEQLSAELQQAKQRVENWKIKGAATKILKADLRRTYERGAVALAQLHQSPNDELFHECRKRSKYHLYHIRLLNNVWPTILSAREAELDELNDYLGDDHDLAVMTQVITGKPEAFGTS 250
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BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Match: A6C0S8_9PLAN (CHAD domain-containing protein n=6 Tax=Gimesia TaxID=1649453 RepID=A6C0S8_9PLAN)

HSP 1 Score: 233 bits (594), Expect = 6.400e-74
Identity = 115/179 (64.25%), Postives = 141/179 (78.77%), Query Frame = 1 
Query:    1 WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPAYVDLFAAFECCFRERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIVAAQLEELDQLNDYLGDDHDLAVMTSQLTSEPERFGAT 537
            WYRDAGR+LSR+RDAES+LESL  L++R+ DPAY  LFA FE   + RKQ++V+EW+DLDQEL+QL  ELQ A Q ++ WKIKG S K+LK GL+ NY+RG EAL +L+  P DELFHECRKRSKY +YH+RLL  VW  I++A+  ELD+LNDYLGDDHDLAVMT   T EPE FG +
Sbjct:   72 WYRDAGRRLSRIRDAESLLESLQSLQERYPDPAYSALFAEFENRLQTRKQKVVDEWVDLDQELKQLSSELQQAKQLVENWKIKGASTKVLKAGLKLNYQRGVEALTQLHQSPSDELFHECRKRSKYHLYHIRLLNAVWPTILSARQAELDELNDYLGDDHDLAVMTQVFTGEPETFGTS 250
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BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Match: A0A517RAC7_9PLAN (CHAD domain protein n=1 Tax=Gimesia alba TaxID=2527973 RepID=A0A517RAC7_9PLAN)

HSP 1 Score: 227 bits (578), Expect = 2.150e-71
Identity = 113/179 (63.13%), Postives = 140/179 (78.21%), Query Frame = 1 
Query:    1 WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPAYVDLFAAFECCFRERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIVAAQLEELDQLNDYLGDDHDLAVMTSQLTSEPERFGAT 537
            WYRDAGR+LSRVRDAESMLESL KLK RF D    DLF+ FE  F  RKQ+LVEEWIDLD ELQ L  +L++ L+ +  WKIKGK+ KI++ GLQ+ Y+RG+EALA L+  P+DELFHECRKRSKY +YH+RL+  VW P++ A++ ELD LNDYLGDDHDL VMT  +T E + FG++
Sbjct:   72 WYRDAGRRLSRVRDAESMLESLQKLKARFSDLEQTDLFSEFENRFLARKQKLVEEWIDLDLELQNLAQQLESELEAVASWKIKGKAAKIIRDGLQQTYQRGSEALATLHQSPNDELFHECRKRSKYHLYHMRLINAVWPPMLTARITELDLLNDYLGDDHDLTVMTQLITGEADSFGSS 250
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BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Match: A0A518I784_9PLAN (CHAD domain protein n=1 Tax=Gimesia fumaroli TaxID=2527976 RepID=A0A518I784_9PLAN)

HSP 1 Score: 226 bits (577), Expect = 3.050e-71
Identity = 113/179 (63.13%), Postives = 140/179 (78.21%), Query Frame = 1 
Query:    1 WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPAYVDLFAAFECCFRERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIVAAQLEELDQLNDYLGDDHDLAVMTSQLTSEPERFGAT 537
            W+RDAGR+LSRVRDAESMLESL KLK RF DP Y  LF+ FE  F  RKQ+LVEEWIDLD ELQ L  +L+T  + +  WKI GK+ KI++ GLQ+ Y+RG+EALA L+  P+DELFHECRKRSKY +YH+RL+   W+PI+ A++ ELD LNDYLGDDHDL VMT  +TSE + FG++
Sbjct:   72 WFRDAGRRLSRVRDAESMLESLQKLKDRFSDPEYGTLFSEFENRFLARKQKLVEEWIDLDSELQTLLLQLETEREAVQGWKINGKAAKIIRDGLQQTYQRGSEALAILHQSPNDELFHECRKRSKYHLYHMRLINAAWQPILTARISELDLLNDYLGDDHDLTVMTQLITSEADSFGSS 250
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BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Match: A0A517WUK0_9PLAN (CHAD domain protein n=2 Tax=Gimesia aquarii TaxID=2527964 RepID=A0A517WUK0_9PLAN)

HSP 1 Score: 187 bits (474), Expect = 8.580e-56
Identity = 94/175 (53.71%), Postives = 130/175 (74.29%), Query Frame = 1 
Query:    1 WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPAYVDLFAAFECCFRERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIVAAQLEELDQLNDYLGDDHDLAVMTSQLTSEPER 525
            WY  AGR+LSRVRDAESMLESL KL +RF D  +  LF+ FE     R+Q +VEEWIDLD+EL++L ++L+ A   +  WKI+G  +KI+  GL++ Y++GA+AL +++  P+DEL H+CRK SK  +YH+RL+   W+P+++A++ ELD+LND LGDDHDLAVMT QL    ER
Sbjct:   72 WYSAAGRRLSRVRDAESMLESLKKLSKRFSDSGHEALFSEFERKLLIRRQNIVEEWIDLDRELEKLSEQLEEARDSVGNWKIEGAVEKIISHGLKQTYQQGAKALKKIHRHPNDELLHDCRKASKNHLYHMRLIVDFWQPVISARIVELDRLNDLLGDDHDLAVMT-QLVQNEER 245
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BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Match: A0A350K426_9PLAN (CHAD domain-containing protein (Fragment) n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A350K426_9PLAN)

HSP 1 Score: 166 bits (420), Expect = 5.890e-49
Identity = 83/126 (65.87%), Postives = 101/126 (80.16%), Query Frame = 1 
Query:    1 WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPAYVDLFAAFECCFRERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKY 378
            WYRDAGR+LSR+RDAES+LESL  L++RF DPAY  LFA FE   + RKQ++V+EW+DLDQEL+QL  ELQ A Q ++ WKIKG S K+LK GL+ NY+RG EAL +L+  P DELFHECRKRSKY
Sbjct:   72 WYRDAGRRLSRIRDAESLLESLQSLQERFPDPAYSALFAEFENRLQTRKQKVVDEWVDLDQELKQLSSELQQAKQLVENWKIKGASTKVLKAGLKLNYQRGVEALTQLHQSPSDELFHECRKRSKY 197
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BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Match: UPI0014244143 (CHAD domain-containing protein n=1 Tax=Rubinisphaera sp. JC658 TaxID=2713176 RepID=UPI0014244143)

HSP 1 Score: 132 bits (332), Expect = 1.600e-34
Identity = 69/179 (38.55%), Postives = 109/179 (60.89%), Query Frame = 1 
Query:    1 WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPAYVDLFAAFECCFRERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIVAAQLEELDQLNDYLGDDHDLAVMTSQLTSEPERFGAT 537
            W+RDAGRK S +RDAE+++E++ +L+ R   P  ++          +R++ L +E ++LD  L +L   L+   Q I EW+   KS   +  G+   Y R  + +   + +P DE +H+ RKR+KY  YH+RLL+ +W+P++  +  ELD+L D LGDDHDLAV+  +L  +PE FG T
Sbjct:   72 WFRDAGRKFSHIRDAETLIETVKRLETRSPSPMEIETLGRLRERLEDRRRALADE-VNLDALLAELRARLEKHRQSIVEWRFSDKSFSAVLDGVHITYDRARDCMQTAFKKPTDENWHQWRKRAKYHWYHVRLLRSLWKPVMKVRSSELDRLADDLGDDHDLAVLMQRLRDQPEEFGGT 249
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BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Match: A0A518CKS2_9PLAN (CHAD domain protein n=1 Tax=Polystyrenella longa TaxID=2528007 RepID=A0A518CKS2_9PLAN)

HSP 1 Score: 131 bits (329), Expect = 4.260e-34
Identity = 67/179 (37.43%), Postives = 111/179 (62.01%), Query Frame = 1 
Query:    1 WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPA--YVDLFAAFECCFRERKQQLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRRGAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIVAAQLEELDQLNDYLGDDHDLAVMTSQLTSEPERFG 531
            WYRD  R+LSR RD E+M+E++  L+    D +    ++ A      RE  +Q+  EW +++  L++L  EL+ A ++I +WK+   S K +++G+++ Y RG + L +    P  E  HE RKR+KY  +HLRLL+  +  ++ A+ +++ QL+DYLGDDHDL+V+T  +  +  RFG
Sbjct:   72 WYRDRSRELSRARDTEAMIEAVEALRTSLADESSEQQEILATTLEVLRESNEQITTEWTEMEDRLEKLVPELKRARKRISDWKLDSVSWKSIRKGIKKTYSRGNKVLKKSVADPTTERLHELRKRTKYHWFHLRLLENTFPKVLEARADQVKQLSDYLGDDHDLSVLTRFILEDANRFG 250
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig120463.4225.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2E7KMM7_9PLAN2.560e-11796.09CHAD domain-containing protein n=6 Tax=Gimesia Tax... [more]
A0A518A1G2_9PLAN5.200e-9882.58CHAD domain protein n=3 Tax=Gimesia panareensis Ta... [more]
A0A355G5K3_9PLAN4.520e-7464.80CHAD domain-containing protein n=2 Tax=Planctomyce... [more]
A6C0S8_9PLAN6.400e-7464.25CHAD domain-containing protein n=6 Tax=Gimesia Tax... [more]
A0A517RAC7_9PLAN2.150e-7163.13CHAD domain protein n=1 Tax=Gimesia alba TaxID=252... [more]
A0A518I784_9PLAN3.050e-7163.13CHAD domain protein n=1 Tax=Gimesia fumaroli TaxID... [more]
A0A517WUK0_9PLAN8.580e-5653.71CHAD domain protein n=2 Tax=Gimesia aquarii TaxID=... [more]
A0A350K426_9PLAN5.890e-4965.87CHAD domain-containing protein (Fragment) n=1 Tax=... [more]
UPI00142441431.600e-3438.55CHAD domain-containing protein n=1 Tax=Rubinisphae... [more]
A0A518CKS2_9PLAN4.260e-3437.43CHAD domain protein n=1 Tax=Polystyrenella longa T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig120463contigM-pyrifera_M_contig120463:2..538 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score236.9
Seed ortholog evalue7.4e-60
Seed eggNOG ortholog344747.PM8797T_05100
Hectar predicted targeting categoryother localisation
EggNOG free text desc.PFAM CHAD domain containing protein
EggNOG OGsCOG5607@1,COG5607@2
COG Functional cat.F
Best tax levelBacteria
Best eggNOG OGNA|NA|NA
Exons1
Model size537
Cds size537
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815724.5050428-CDS-M-pyrifera_M_contig120463:1..5381622815724.5050428-CDS-M-pyrifera_M_contig120463:1..538Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120463 2..538 +
1692277539.4456172-CDS-M-pyrifera_M_contig120463:1..5381692277539.4456172-CDS-M-pyrifera_M_contig120463:1..538Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120463 2..538 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig120463.4225.1prot_M-pyrifera_M_contig120463.4225.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig120463 2..538 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig120463.4225.1

>prot_M-pyrifera_M_contig120463.4225.1 ID=prot_M-pyrifera_M_contig120463.4225.1|Name=mRNA_M-pyrifera_M_contig120463.4225.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=179bp
WYRDAGRKLSRVRDAESMLESLAKLKQRFDDPAYVDLFAAFECCFRERKQ
QLVEEWIDLDQELQQLCDELQTALQQIDEWKIKGKSDKILKRGLQRNYRR
GAEALAELYVQPHDELFHECRKRSKYLMYHLRLLKKVWEPIVAAQLEELD
QLNDYLGDDHDLAVMTSQLTSEPERFGAT
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mRNA from alignment at M-pyrifera_M_contig120463:2..538+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig120463.4225.1 ID=mRNA_M-pyrifera_M_contig120463.4225.1|Name=mRNA_M-pyrifera_M_contig120463.4225.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=537bp|location=Sequence derived from alignment at M-pyrifera_M_contig120463:2..538+ (Macrocystis pyrifera P11B4 male)
TGGTATCGCGATGCAGGACGCAAACTGTCCCGCGTCCGCGATGCAGAATC
GATGCTGGAATCTCTGGCGAAACTCAAGCAACGCTTCGACGATCCCGCAT
ACGTAGACCTGTTTGCCGCTTTTGAATGCTGTTTCCGAGAACGCAAGCAG
CAACTGGTGGAAGAGTGGATTGACCTGGATCAGGAACTGCAGCAGCTGTG
TGACGAACTGCAGACCGCACTCCAGCAGATTGACGAGTGGAAGATCAAAG
GCAAATCAGACAAGATTCTCAAACGCGGATTGCAGCGGAATTACCGTCGT
GGTGCTGAGGCTCTGGCGGAACTGTATGTGCAACCGCACGATGAACTCTT
TCACGAGTGCCGTAAACGCAGCAAGTATCTGATGTATCACCTGCGCCTGC
TGAAAAAAGTCTGGGAGCCGATTGTGGCAGCGCAGCTTGAGGAACTCGAT
CAGTTAAATGATTACCTCGGCGACGATCACGATCTGGCGGTGATGACCAG
TCAGCTGACCAGCGAACCGGAACGGTTCGGCGCGACC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig120463:2..538+

>mRNA_M-pyrifera_M_contig120463.4225.1 ID=mRNA_M-pyrifera_M_contig120463.4225.1|Name=mRNA_M-pyrifera_M_contig120463.4225.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1074bp|location=Sequence derived from alignment at M-pyrifera_M_contig120463:2..538+ (Macrocystis pyrifera P11B4 male)
TGGTATCGCGATGCAGGACGCAAACTGTCCCGCGTCCGCGATGCAGAATC
GATGCTGGAATCTCTGGCGAAACTCAAGCAACGCTTCGACGATCCCGCAT
ACGTAGACCTGTTTGCCGCTTTTGAATGCTGTTTCCGAGAACGCAAGCAG
CAACTGGTGGAAGAGTGGATTGACCTGGATCAGGAACTGCAGCAGCTGTG
TGACGAACTGCAGACCGCACTCCAGCAGATTGACGAGTGGAAGATCAAAG
GCAAATCAGACAAGATTCTCAAACGCGGATTGCAGCGGAATTACCGTCGT
GGTGCTGAGGCTCTGGCGGAACTGTATGTGCAACCGCACGATGAACTCTT
TCACGAGTGCCGTAAACGCAGCAAGTATCTGATGTATCACCTGCGCCTGC
TGAAAAAAGTCTGGGAGCCGATTGTGGCAGCGCAGCTTGAGGAACTCGAT
CAGTTAAATGATTACCTCGGCGACGATCACGATCTGGCGGTGATGACCAG
TCAGCTGACCAGCGAACCGGAACGGTTCGGCGCGACCTGGTATCGCGATG
CAGGACGCAAACTGTCCCGCGTCCGCGATGCAGAATCGATGCTGGAATCT
CTGGCGAAACTCAAGCAACGCTTCGACGATCCCGCATACGTAGACCTGTT
TGCCGCTTTTGAATGCTGTTTCCGAGAACGCAAGCAGCAACTGGTGGAAG
AGTGGATTGACCTGGATCAGGAACTGCAGCAGCTGTGTGACGAACTGCAG
ACCGCACTCCAGCAGATTGACGAGTGGAAGATCAAAGGCAAATCAGACAA
GATTCTCAAACGCGGATTGCAGCGGAATTACCGTCGTGGTGCTGAGGCTC
TGGCGGAACTGTATGTGCAACCGCACGATGAACTCTTTCACGAGTGCCGT
AAACGCAGCAAGTATCTGATGTATCACCTGCGCCTGCTGAAAAAAGTCTG
GGAGCCGATTGTGGCAGCGCAGCTTGAGGAACTCGATCAGTTAAATGATT
ACCTCGGCGACGATCACGATCTGGCGGTGATGACCAGTCAGCTGACCAGC
GAACCGGAACGGTTCGGCGCGACC
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