mRNA_M-pyrifera_M_contig120172.4160.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig120172.4160.1
Unique NamemRNA_M-pyrifera_M_contig120172.4160.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Match: A0A5C7T8D0_PSESP (Radical SAM protein (Fragment) n=1 Tax=Pseudomonas sp. TaxID=306 RepID=A0A5C7T8D0_PSESP)

HSP 1 Score: 292 bits (747), Expect = 1.180e-98
Identity = 143/177 (80.79%), Postives = 158/177 (89.27%), Query Frame = 1 
Query:    4 ASAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCGFIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQQYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGRLDSRPIEDVMAEAERL 534
            A+ KVGFVSLGCPKA VDSERI+TQLRL+GYE+V TYEDADVVVVNTCGFIDSAKAESL TIGEA++ENGKVIVTGC+GV E+AIR VHP+VL+VTGPQQYE VV AVH   P    HDPF DLVPPQGIKLTPRHYAYLKISEGCNH CSFCIIPSMRG+L SRP+ DV++EA+RL
Sbjct:    4 ATPKVGFVSLGCPKATVDSERILTQLRLEGYEIVPTYEDADVVVVNTCGFIDSAKAESLDTIGEAIAENGKVIVTGCMGVSENAIRDVHPSVLAVTGPQQYEQVVNAVHEVIPPKKDHDPFVDLVPPQGIKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGDVLSEAQRL 180
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BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Match: A0A7X6Q4Q5_9BURK (Radical SAM protein (Fragment) n=1 Tax=Alcaligenaceae bacterium TaxID=2021368 RepID=A0A7X6Q4Q5_9BURK)

HSP 1 Score: 290 bits (742), Expect = 2.420e-97
Identity = 143/177 (80.79%), Postives = 156/177 (88.14%), Query Frame = 1 
Query:    4 ASAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCGFIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQQYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGRLDSRPIEDVMAEAERL 534
            +S KVGFVSLGCPKALVDSERI+TQLR +GYE+  TYE ADVVVVNTCGFIDSAKAESL  IGEAL+ENG+VIVTGC+GV ED IR VHP+VLSVTGPQQYE VV AVH  AP ++ HDPF DLVPPQGI+LTPRHYAYLKISEGCNH C+FCIIPSMRGRL SRPI DVM EA+RL
Sbjct:    2 SSPKVGFVSLGCPKALVDSERILTQLRSEGYEISPTYEGADVVVVNTCGFIDSAKAESLDAIGEALAENGRVIVTGCMGVEEDTIRQVHPSVLSVTGPQQYEEVVRAVHEAAPPEIDHDPFVDLVPPQGIRLTPRHYAYLKISEGCNHSCAFCIIPSMRGRLVSRPIGDVMDEAKRL 178
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BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Match: A0A359DES5_PSESP (30S ribosomal protein S12 methylthiotransferase RimO (Fragment) n=1 Tax=Pseudomonas sp. TaxID=306 RepID=A0A359DES5_PSESP)

HSP 1 Score: 292 bits (748), Expect = 3.610e-97
Identity = 142/178 (79.78%), Postives = 159/178 (89.33%), Query Frame = 1 
Query:    1 PASAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCGFIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQQYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGRLDSRPIEDVMAEAERL 534
            PA+ KVGFVSLGCPKALVDSERI+TQLR++GYE+V+TY+DADVVVVNTCGFIDSAKAESL  IGEAL+ENGKVIVTGC+GV E AIR VHP+VLSVTGPQQYE V+ AVH   P +  H+P  DLVPPQGIKLTPRHYAYLKISEGCNH CSFCIIPSMRG+L SRP+ DV++EAERL
Sbjct:    4 PATPKVGFVSLGCPKALVDSERILTQLRMEGYEVVSTYQDADVVVVNTCGFIDSAKAESLEVIGEALAENGKVIVTGCMGVEESAIRNVHPSVLSVTGPQQYEQVLNAVHEVVPPNTEHNPLIDLVPPQGIKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGDVLSEAERL 181
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BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Match: A0A1H5TMJ4_9GAMM (Ribosomal protein S12 methylthiotransferase RimO n=13 Tax=Alcanivoracaceae TaxID=224372 RepID=A0A1H5TMJ4_9GAMM)

HSP 1 Score: 296 bits (759), Expect = 4.380e-97
Identity = 146/176 (82.95%), Postives = 155/176 (88.07%), Query Frame = 1 
Query:    7 SAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCGFIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQQYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGRLDSRPIEDVMAEAERL 534
            S KVGF+SLGCPKALVDSERI+TQLR +GYE+  +Y+DADVVVVNTCGFID AKAESL TIGEALSENGKVIVTGC+GV ED IR VHP+VLSVTGPQQYE VV AVH   P    HDPF DLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRG+LDSRPI DVM EAERL
Sbjct:    2 SGKVGFISLGCPKALVDSERILTQLRTEGYEVTPSYDDADVVVVNTCGFIDDAKAESLETIGEALSENGKVIVTGCMGVEEDTIRKVHPSVLSVTGPQQYEQVVNAVHQVVPPSQNHDPFLDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGKLDSRPIGDVMDEAERL 177
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BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Match: A0A0F2R3R7_9PSED (Ribosomal protein S12 methylthiotransferase (Fragment) n=1 Tax=Pseudomonas sp. BRH_c35 TaxID=1629711 RepID=A0A0F2R3R7_9PSED)

HSP 1 Score: 289 bits (740), Expect = 1.640e-96
Identity = 138/177 (77.97%), Postives = 158/177 (89.27%), Query Frame = 1 
Query:    4 ASAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCGFIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQQYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGRLDSRPIEDVMAEAERL 534
            A+ KVGF+SLGCPKA VDSERI+TQLR++GY++V +YEDADVVVVNTCGFIDSAKAESL  IGEA++ENGKVIVTGC+GV E+ IR VHP+VL+VTGPQQYE VV AVH   P ++ HDPF DLVPPQGIKLTPRHYAYLKISEGCNH CSFCIIPSMRG+L SRP+ DV++EAERL
Sbjct:    4 ATPKVGFISLGCPKATVDSERILTQLRMEGYQIVPSYEDADVVVVNTCGFIDSAKAESLDAIGEAIAENGKVIVTGCMGVDENNIRGVHPSVLAVTGPQQYEQVVNAVHEVVPPNIEHDPFVDLVPPQGIKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGDVLSEAERL 180
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BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Match: A0A7V8TT04_9GAMM (Ribosomal protein S12 methylthiotransferase RimO n=1 Tax=Ectothiorhodospiraceae bacterium WFHF3C12 TaxID=1914406 RepID=A0A7V8TT04_9GAMM)

HSP 1 Score: 294 bits (753), Expect = 3.860e-96
Identity = 145/177 (81.92%), Postives = 156/177 (88.14%), Query Frame = 1 
Query:    4 ASAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCGFIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQQYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGRLDSRPIEDVMAEAERL 534
            A+ KVGFVSLGCPKALVDSERI+TQLR DGY++V TYEDADVVVVNTCGFID AKAESL  IGEA+  NG+VIVTGCLGV   AI+  HP VL+VTGPQQYEAVVGAVH HAP+  AHDPFTDLVPPQGIKLTPRHYAYLKISEGCNH+CSFCIIPSMRG L SRP  DV+AEAERL
Sbjct:    4 AAPKVGFVSLGCPKALVDSERILTQLRTDGYQVVPTYEDADVVVVNTCGFIDEAKAESLEAIGEAVEANGRVIVTGCLGVESAAIQQAHPRVLAVTGPQQYEAVVGAVHEHAPMTRAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHRCSFCIIPSMRGDLVSRPAGDVLAEAERL 180
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BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Match: A0A3N5J4R0_9GAMM (Radical SAM protein (Fragment) n=1 Tax=Xanthomonadales bacterium TaxID=2006849 RepID=A0A3N5J4R0_9GAMM)

HSP 1 Score: 286 bits (731), Expect = 1.660e-95
Identity = 141/177 (79.66%), Postives = 151/177 (85.31%), Query Frame = 1 
Query:    4 ASAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCGFIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQQYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGRLDSRPIEDVMAEAERL 534
            A+ KVGFVSLGCPKALVDSERI+TQLR DGY +   Y  ADVVVVNTCGFIDSAKAESL  IGEAL  NG+VIVTGC+GV E  IRA+HP VL+VTGPQQYEAVV AVH HAP+  AHDPF DLVPPQGIKLTPRHYAYLKISEGCNH C+FCIIPSMRGRL SRP  DV+ EAERL
Sbjct:    8 ATPKVGFVSLGCPKALVDSERILTQLRTDGYRISPDYAGADVVVVNTCGFIDSAKAESLDAIGEALEANGRVIVTGCMGVDEAQIRALHPRVLAVTGPQQYEAVVSAVHRHAPMPAAHDPFVDLVPPQGIKLTPRHYAYLKISEGCNHHCTFCIIPSMRGRLVSRPAGDVLGEAERL 184
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BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Match: K0CDM1_ALCDB (Ribosomal protein S12 methylthiotransferase RimO n=10 Tax=Alcanivorax TaxID=59753 RepID=K0CDM1_ALCDB)

HSP 1 Score: 292 bits (748), Expect = 1.840e-95
Identity = 143/176 (81.25%), Postives = 154/176 (87.50%), Query Frame = 1 
Query:    7 SAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCGFIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQQYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGRLDSRPIEDVMAEAERL 534
            S KVGF+SLGCPKALVDSERI+TQLR +GYE+  +Y+DADVVVVNTCGFID AKAESL  IGEALSENGKVIVTGC+GV ED IR VHPAVLSVTGPQQYE VV AVH   P    H+PFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIP MRG+LDSRP+ DV+ EAERL
Sbjct:    2 SGKVGFISLGCPKALVDSERILTQLRTEGYEVTPSYDDADVVVVNTCGFIDDAKAESLEAIGEALSENGKVIVTGCMGVEEDNIRKVHPAVLSVTGPQQYEQVVNAVHQVVPPRADHNPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPGMRGKLDSRPVGDVLDEAERL 177
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BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Match: A0A1H7K2F0_9GAMM (Ribosomal protein S12 methylthiotransferase RimO n=3 Tax=Pseudomonadaceae TaxID=135621 RepID=A0A1H7K2F0_9GAMM)

HSP 1 Score: 291 bits (746), Expect = 3.910e-95
Identity = 141/177 (79.66%), Postives = 157/177 (88.70%), Query Frame = 1 
Query:    4 ASAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCGFIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQQYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGRLDSRPIEDVMAEAERL 534
            AS KVGFVSLGCPKALVDSERI+TQLR++GYE+V TY+DADVVVVNTCGFIDSAKAESL  IGEA++ENGKVIVTGC+GV E +IR VHP VL+VTGPQQYE VV AVH   P    HDPFTDLVPPQG+KLTPRHYAYLKISEGCNH CSFCIIPSMRG+L SRP+ DV++EA+RL
Sbjct:    4 ASPKVGFVSLGCPKALVDSERILTQLRMEGYEVVPTYQDADVVVVNTCGFIDSAKAESLDAIGEAIAENGKVIVTGCMGVEEGSIREVHPNVLAVTGPQQYEQVVNAVHEVVPPKTEHDPFTDLVPPQGVKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGDVLSEAQRL 180
[Close]
BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Match: A0A2I0CSG2_9PSED (Ribosomal protein S12 methylthiotransferase RimO n=1 Tax=Pseudomonas pharmacofabricae TaxID=1905276 RepID=A0A2I0CSG2_9PSED)

HSP 1 Score: 291 bits (745), Expect = 5.700e-95
Identity = 142/177 (80.23%), Postives = 158/177 (89.27%), Query Frame = 1 
Query:    4 ASAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCGFIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQQYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHKCSFCIIPSMRGRLDSRPIEDVMAEAERL 534
            +S KVGFVSLGCPKA VDSERI+TQLR +GY++V TYEDADVVVVNTCGFIDSAKAESL TIGEA++ENGKVIVTGC+GV EDAIR VHP+VL+VTGPQQYE VV AVH   P  + HDPF DLVPPQGIKLTPRHYAYLKISEGCNH CSFCIIPSMRG+L SRP+ +V++EAERL
Sbjct:    5 SSPKVGFVSLGCPKATVDSERILTQLRQEGYQIVPTYEDADVVVVNTCGFIDSAKAESLDTIGEAIAENGKVIVTGCMGVAEDAIRNVHPSVLAVTGPQQYEQVVQAVHEVVPPKIDHDPFVDLVPPQGIKLTPRHYAYLKISEGCNHSCSFCIIPSMRGKLVSRPVGEVLSEAERL 181
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig120172.4160.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5C7T8D0_PSESP1.180e-9880.79Radical SAM protein (Fragment) n=1 Tax=Pseudomonas... [more]
A0A7X6Q4Q5_9BURK2.420e-9780.79Radical SAM protein (Fragment) n=1 Tax=Alcaligenac... [more]
A0A359DES5_PSESP3.610e-9779.7830S ribosomal protein S12 methylthiotransferase Ri... [more]
A0A1H5TMJ4_9GAMM4.380e-9782.95Ribosomal protein S12 methylthiotransferase RimO n... [more]
A0A0F2R3R7_9PSED1.640e-9677.97Ribosomal protein S12 methylthiotransferase (Fragm... [more]
A0A7V8TT04_9GAMM3.860e-9681.92Ribosomal protein S12 methylthiotransferase RimO n... [more]
A0A3N5J4R0_9GAMM1.660e-9579.66Radical SAM protein (Fragment) n=1 Tax=Xanthomonad... [more]
K0CDM1_ALCDB1.840e-9581.25Ribosomal protein S12 methylthiotransferase RimO n... [more]
A0A1H7K2F0_9GAMM3.910e-9579.66Ribosomal protein S12 methylthiotransferase RimO n... [more]
A0A2I0CSG2_9PSED5.700e-9580.23Ribosomal protein S12 methylthiotransferase RimO n... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig120172contigM-pyrifera_M_contig120172:2..535 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score300.1
Seed ortholog evalue7.1e-79
Seed eggNOG ortholog1177181.T9A_00130
Preferred namerimO
KEGG rclassRC00003,RC03217
KEGG koko:K14441
KEGG ReactionR10652
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12
EggNOG OGs1MU7N@1224,1RN46@1236,1XI9G@135619,COG0621@1,COG0621@2
EC2.8.4.4
COG Functional cat.H
Best tax levelOceanospirillales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03009
Exons1
Model size534
Cds size534
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815721.2667143-CDS-M-pyrifera_M_contig120172:1..5351622815721.2667143-CDS-M-pyrifera_M_contig120172:1..535Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120172 2..535 -
1692277537.8467731-CDS-M-pyrifera_M_contig120172:1..5351692277537.8467731-CDS-M-pyrifera_M_contig120172:1..535Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig120172 2..535 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig120172.4160.1prot_M-pyrifera_M_contig120172.4160.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig120172 2..535 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig120172.4160.1

>prot_M-pyrifera_M_contig120172.4160.1 ID=prot_M-pyrifera_M_contig120172.4160.1|Name=mRNA_M-pyrifera_M_contig120172.4160.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=178bp
PASAKVGFVSLGCPKALVDSERIMTQLRLDGYELVATYEDADVVVVNTCG
FIDSAKAESLGTIGEALSENGKVIVTGCLGVHEDAIRAVHPAVLSVTGPQ
QYEAVVGAVHAHAPIDVAHDPFTDLVPPQGIKLTPRHYAYLKISEGCNHK
CSFCIIPSMRGRLDSRPIEDVMAEAERL
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mRNA from alignment at M-pyrifera_M_contig120172:2..535-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig120172.4160.1 ID=mRNA_M-pyrifera_M_contig120172.4160.1|Name=mRNA_M-pyrifera_M_contig120172.4160.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=534bp|location=Sequence derived from alignment at M-pyrifera_M_contig120172:2..535- (Macrocystis pyrifera P11B4 male)
CCCGCGTCGGCGAAAGTCGGATTCGTCAGCCTGGGTTGCCCGAAGGCGCT
CGTCGACTCCGAACGTATCATGACGCAGCTGCGTCTCGATGGTTACGAGC
TTGTCGCTACTTACGAGGATGCCGATGTCGTGGTCGTGAACACATGCGGC
TTCATCGACAGCGCGAAAGCGGAATCGCTCGGAACGATCGGCGAAGCCCT
GAGCGAGAACGGCAAGGTGATCGTGACAGGCTGTCTGGGCGTCCACGAAG
ACGCGATCCGCGCCGTGCATCCGGCCGTGCTCAGCGTCACGGGTCCGCAA
CAATACGAGGCCGTCGTCGGCGCCGTGCACGCCCATGCGCCAATCGACGT
GGCCCACGATCCGTTTACCGATCTCGTGCCACCGCAGGGGATCAAGCTCA
CGCCACGACATTACGCGTATCTGAAAATCTCCGAGGGCTGCAATCACAAA
TGCAGCTTCTGCATCATCCCGTCGATGCGCGGGCGCCTCGACAGCCGTCC
GATCGAGGACGTCATGGCCGAGGCCGAACGGCTC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig120172:2..535-

>mRNA_M-pyrifera_M_contig120172.4160.1 ID=mRNA_M-pyrifera_M_contig120172.4160.1|Name=mRNA_M-pyrifera_M_contig120172.4160.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1068bp|location=Sequence derived from alignment at M-pyrifera_M_contig120172:2..535- (Macrocystis pyrifera P11B4 male)
CCCGCGTCGGCGAAAGTCGGATTCGTCAGCCTGGGTTGCCCGAAGGCGCT
CGTCGACTCCGAACGTATCATGACGCAGCTGCGTCTCGATGGTTACGAGC
TTGTCGCTACTTACGAGGATGCCGATGTCGTGGTCGTGAACACATGCGGC
TTCATCGACAGCGCGAAAGCGGAATCGCTCGGAACGATCGGCGAAGCCCT
GAGCGAGAACGGCAAGGTGATCGTGACAGGCTGTCTGGGCGTCCACGAAG
ACGCGATCCGCGCCGTGCATCCGGCCGTGCTCAGCGTCACGGGTCCGCAA
CAATACGAGGCCGTCGTCGGCGCCGTGCACGCCCATGCGCCAATCGACGT
GGCCCACGATCCGTTTACCGATCTCGTGCCACCGCAGGGGATCAAGCTCA
CGCCACGACATTACGCGTATCTGAAAATCTCCGAGGGCTGCAATCACAAA
TGCAGCTTCTGCATCATCCCGTCGATGCGCGGGCGCCTCGACAGCCGTCC
GATCGAGGACGTCATGGCCGAGGCCGAACGGCTCCCCGCGTCGGCGAAAG
TCGGATTCGTCAGCCTGGGTTGCCCGAAGGCGCTCGTCGACTCCGAACGT
ATCATGACGCAGCTGCGTCTCGATGGTTACGAGCTTGTCGCTACTTACGA
GGATGCCGATGTCGTGGTCGTGAACACATGCGGCTTCATCGACAGCGCGA
AAGCGGAATCGCTCGGAACGATCGGCGAAGCCCTGAGCGAGAACGGCAAG
GTGATCGTGACAGGCTGTCTGGGCGTCCACGAAGACGCGATCCGCGCCGT
GCATCCGGCCGTGCTCAGCGTCACGGGTCCGCAACAATACGAGGCCGTCG
TCGGCGCCGTGCACGCCCATGCGCCAATCGACGTGGCCCACGATCCGTTT
ACCGATCTCGTGCCACCGCAGGGGATCAAGCTCACGCCACGACATTACGC
GTATCTGAAAATCTCCGAGGGCTGCAATCACAAATGCAGCTTCTGCATCA
TCCCGTCGATGCGCGGGCGCCTCGACAGCCGTCCGATCGAGGACGTCATG
GCCGAGGCCGAACGGCTC
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