mRNA_M-pyrifera_M_contig119977.4120.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig119977.4120.1
Unique NamemRNA_M-pyrifera_M_contig119977.4120.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Match: A0A3M8G140_9BACT (Transcription termination factor Rho n=1 Tax=Balneola sp. TaxID=2024824 RepID=A0A3M8G140_9BACT)

HSP 1 Score: 301 bits (772), Expect = 1.530e-99
Identity = 155/174 (89.08%), Postives = 164/174 (94.25%), Query Frame = 1 
Query:    1 WIGVGGNPGGTGMRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALSTHHKDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMTLGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 522
            WI +G  P  T MRV+DLVAPIGKGQRALIVAPPRTGKTVLLKQIAN+LS +H DIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSH+RI EM LGYAKRKAEMG+DA+LLIDS+TRLGRAYNA+QTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL
Sbjct:  137 WIRLGELPDDTEMRVIDLVAPIGKGQRALIVAPPRTGKTVLLKQIANSLSANHPDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHIRITEMALGYAKRKAEMGQDAILLIDSLTRLGRAYNAIQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 310
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BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Match: A0A3D1GC52_9BACT (Transcription termination factor Rho n=1 Tax=Balneolaceae bacterium TaxID=2053516 RepID=A0A3D1GC52_9BACT)

HSP 1 Score: 301 bits (772), Expect = 1.630e-99
Identity = 155/174 (89.08%), Postives = 165/174 (94.83%), Query Frame = 1 
Query:    1 WIGVGGNPGGTGMRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALSTHHKDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMTLGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 522
            W+ +G NP    MRV+DLVAPIGKGQRALIVAPPRTGKTVLLKQIAN++S +H DIHLSVLLVDERPEEVTDF+RSTDAEVFASSNDKKTQSHVRI EMTLGYAKRKAEMGEDAVLLIDS+TRLGRAYNA+Q+NSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL
Sbjct:  139 WMRMGDNPEDIEMRVIDLVAPIGKGQRALIVAPPRTGKTVLLKQIANSISQNHPDIHLSVLLVDERPEEVTDFMRSTDAEVFASSNDKKTQSHVRITEMTLGYAKRKAEMGEDAVLLIDSLTRLGRAYNAIQSNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 312
[Close]
BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Match: A0A2E6N2V2_9BACT (Transcription termination factor Rho n=2 Tax=Balneola sp. TaxID=2024824 RepID=A0A2E6N2V2_9BACT)

HSP 1 Score: 290 bits (742), Expect = 5.990e-95
Identity = 146/174 (83.91%), Postives = 163/174 (93.68%), Query Frame = 1 
Query:    1 WIGVGGNPGGTGMRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALSTHHKDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMTLGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 522
            WI +G     T MRV+DLVAPIGKGQRALIVAPPRTGKTVLLKQIA++++ HH DIHLS+LLVDERPEEVTDFIRS++AE+FASSND+KTQSH+RI EMTLGYAKRKAEMG+DAV+LIDS+TRLGRAYNA+Q+NSGRTLSGGLDIRALEIPKKIFGSARKIE GGSLTIIATCL
Sbjct:  141 WIRLGDRAQDTEMRVIDLVAPIGKGQRALIVAPPRTGKTVLLKQIADSIAHHHPDIHLSILLVDERPEEVTDFIRSSNAEIFASSNDRKTQSHIRITEMTLGYAKRKAEMGQDAVILIDSLTRLGRAYNAIQSNSGRTLSGGLDIRALEIPKKIFGSARKIESGGSLTIIATCL 314
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BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Match: A0A356WZ78_9BACT (Transcription termination factor Rho (Fragment) n=1 Tax=Balneolaceae bacterium TaxID=2053516 RepID=A0A356WZ78_9BACT)

HSP 1 Score: 281 bits (719), Expect = 1.150e-93
Identity = 143/174 (82.18%), Postives = 159/174 (91.38%), Query Frame = 1 
Query:    1 WIGVGGNPGGTGMRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALSTHHKDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMTLGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 522
            WI +G  P    MRV+DLV+PIGKGQRALIVAPPRTGKTVLLK+IA +L+ ++ DIH+SVLLVDERPEEVTDFIR+T+AEVFASSNDK T SH+RI EM LGYAKRKAEMGED+VLLIDS+TRLGRAYNA+Q+NSGRTLSGGLDIRALEIPKKIFGSARK EGGGSLTIIATCL
Sbjct:   55 WIKLGHQPDDVEMRVIDLVSPIGKGQRALIVAPPRTGKTVLLKKIAKSLNDNYDDIHVSVLLVDERPEEVTDFIRTTNAEVFASSNDKNTDSHIRITEMALGYAKRKAEMGEDSVLLIDSLTRLGRAYNAIQSNSGRTLSGGLDIRALEIPKKIFGSARKFEGGGSLTIIATCL 228
[Close]
BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Match: A0A350PGK9_9BACT (Transcription termination factor Rho n=8 Tax=Bacteroidetes/Chlorobi group TaxID=68336 RepID=A0A350PGK9_9BACT)

HSP 1 Score: 286 bits (731), Expect = 2.300e-93
Identity = 148/174 (85.06%), Postives = 159/174 (91.38%), Query Frame = 1 
Query:    1 WIGVGGNPGGTGMRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALSTHHKDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMTLGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 522
            WI +G       MRV+DLVAPIGKGQRALIVAPPRTGKTV+LKQIA +++ +H DI LSVLLVDERPEEVTDFIRSTDAEVFASSND KTQSH+RI EM LGYAKRKAEMGED+VLLIDS+TRLGRAYNA+QTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL
Sbjct:  135 WIKLGEPSENIEMRVIDLVAPIGKGQRALIVAPPRTGKTVMLKQIAKSITENHPDIPLSVLLVDERPEEVTDFIRSTDAEVFASSNDNKTQSHIRITEMALGYAKRKAEMGEDSVLLIDSLTRLGRAYNAIQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 308
[Close]
BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Match: A0A2D8CI85_9BACT (Transcription termination factor Rho n=8 Tax=Balneolaceae TaxID=1813606 RepID=A0A2D8CI85_9BACT)

HSP 1 Score: 282 bits (722), Expect = 5.130e-92
Identity = 148/175 (84.57%), Postives = 160/175 (91.43%), Query Frame = 1 
Query:    1 WIGVGGNPGGTGMRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALSTHHKD-IHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMTLGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 522
            WI +G N     MRVVDLVAPIGKGQR+LIVAPPRTGKTVLLK+IA +L+ +H D IH+SVLLVDERPEEVTDFIRST AEVFASSNDK TQSH+RI EM LGYAKRKAEMGED+VLLIDS+TRLGRAYNA+Q+NSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL
Sbjct:  133 WIKLGFNADDVEMRVVDLVAPIGKGQRSLIVAPPRTGKTVLLKKIAKSLNDNHSDDIHVSVLLVDERPEEVTDFIRSTQAEVFASSNDKNTQSHIRITEMALGYAKRKAEMGEDSVLLIDSITRLGRAYNAVQSNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 307
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BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Match: A0A316TPG3_9BACT (Transcription termination factor Rho n=1 Tax=Rhodohalobacter mucosus TaxID=2079485 RepID=A0A316TPG3_9BACT)

HSP 1 Score: 276 bits (705), Expect = 2.210e-89
Identity = 142/174 (81.61%), Postives = 156/174 (89.66%), Query Frame = 1 
Query:    1 WIGVGGNPGGTGMRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALSTHHKDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMTLGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 522
            +I +G NP    +RV+DLVAPIGKGQRALIVAPPRTGKTVLLK+IA +L+ +H +IH  +LLVDERPEEVTDF+RST+AEVFASSNDK T+SHVRI EM LGY KRKAE GE AVLLIDS+TRLGRAYNA QTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL
Sbjct:  139 YIQLGHNPDDIELRVIDLVAPIGKGQRALIVAPPRTGKTVLLKKIAKSLTDNHPNIHTGILLVDERPEEVTDFLRSTNAEVFASSNDKPTESHVRISEMALGYVKRKAEFGEHAVLLIDSLTRLGRAYNAAQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 312
[Close]
BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Match: A0A6M1SSL6_9BACT (Transcription termination factor Rho n=1 Tax=Balneolaceae bacterium YR4-1 TaxID=2709311 RepID=A0A6M1SSL6_9BACT)

HSP 1 Score: 273 bits (698), Expect = 1.820e-88
Identity = 138/162 (85.19%), Postives = 150/162 (92.59%), Query Frame = 1 
Query:   37 MRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALSTHHKDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMTLGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 522
            MRV+DLVAPIGKGQRALIVAPPRTGKTVLLK++A ++  H+ DIH SVLLVDERPEEVTDF+R+T AEVFASSNDK T SH+R+ EM LGY KRKAEMGEDAVLLIDS+TRLGRAYNA Q+NSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL
Sbjct:  140 MRVIDLVAPIGKGQRALIVAPPRTGKTVLLKKMAESVCEHYPDIHTSVLLVDERPEEVTDFLRTTKAEVFASSNDKPTTSHLRVTEMALGYVKRKAEMGEDAVLLIDSLTRLGRAYNAAQSNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 301
[Close]
BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Match: A0A352SK74_9BACT (Transcription termination factor Rho n=3 Tax=Bacteroidetes/Chlorobi group TaxID=68336 RepID=A0A352SK74_9BACT)

HSP 1 Score: 272 bits (696), Expect = 4.910e-88
Identity = 136/168 (80.95%), Postives = 150/168 (89.29%), Query Frame = 1 
Query:   19 NPGGTGMRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALSTHHKDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMTLGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 522
            NP    +R +DL+APIGKGQRALIVAPPRTGKTVLLKQIA AL+ HH ++H S+LLVDERPEEVTDF+R+T AEVFASSND+ TQSHVRI EM LGYAKRKAE G+DAVLLIDS+TRLGRAYNA QT SGRTLSGGLDIRALE+PK+IFGSAR  EGGGSLTIIATCL
Sbjct:  147 NPEEVELRALDLIAPIGKGQRALIVAPPRTGKTVLLKQIAGALTEHHPEVHTSILLVDERPEEVTDFLRTTSAEVFASSNDRSTQSHVRIAEMALGYAKRKAESGQDAVLLIDSLTRLGRAYNAAQTGSGRTLSGGLDIRALEVPKRIFGSARAFEGGGSLTIIATCL 314
[Close]
BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Match: A0A2N0VGY7_9BACT (Transcription termination factor Rho n=2 Tax=Rhodohalobacter TaxID=1974209 RepID=A0A2N0VGY7_9BACT)

HSP 1 Score: 269 bits (688), Expect = 6.650e-87
Identity = 135/162 (83.33%), Postives = 151/162 (93.21%), Query Frame = 1 
Query:   37 MRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALSTHHKDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMTLGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 522
            +RV+DLV+PIG+GQRALIVAPPRTGKTVLLK+IA AL+ HH DI+  +LLVDERPEEVTDF+RST+A+VFASSNDK T+SHVRI EM LG+ KRKAE GE AVLLIDS+TRLGRAYNA+Q+NSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL
Sbjct:  144 LRVIDLVSPIGRGQRALIVAPPRTGKTVLLKKIARALTEHHPDIYTGILLVDERPEEVTDFLRSTNADVFASSNDKPTESHVRISEMALGHVKRKAEAGEHAVLLIDSLTRLGRAYNAVQSNSGRTLSGGLDIRALEIPKKIFGSARKIEGGGSLTIIATCL 305
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig119977.4120.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3M8G140_9BACT1.530e-9989.08Transcription termination factor Rho n=1 Tax=Balne... [more]
A0A3D1GC52_9BACT1.630e-9989.08Transcription termination factor Rho n=1 Tax=Balne... [more]
A0A2E6N2V2_9BACT5.990e-9583.91Transcription termination factor Rho n=2 Tax=Balne... [more]
A0A356WZ78_9BACT1.150e-9382.18Transcription termination factor Rho (Fragment) n=... [more]
A0A350PGK9_9BACT2.300e-9385.06Transcription termination factor Rho n=8 Tax=Bacte... [more]
A0A2D8CI85_9BACT5.130e-9284.57Transcription termination factor Rho n=8 Tax=Balne... [more]
A0A316TPG3_9BACT2.210e-8981.61Transcription termination factor Rho n=1 Tax=Rhodo... [more]
A0A6M1SSL6_9BACT1.820e-8885.19Transcription termination factor Rho n=1 Tax=Balne... [more]
A0A352SK74_9BACT4.910e-8880.95Transcription termination factor Rho n=3 Tax=Bacte... [more]
A0A2N0VGY7_9BACT6.650e-8783.33Transcription termination factor Rho n=2 Tax=Rhodo... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig119977contigM-pyrifera_M_contig119977:1..522 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score289.3
Seed ortholog evalue1.2e-75
Seed eggNOG ortholog1121104.AQXH01000001_gene2124
Preferred namerho
KEGG koko:K03628
KEGG Pathwayko03018,map03018
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template
EggNOG OGs1IPNZ@117747,4NEFP@976,COG1158@1,COG1158@2
COG Functional cat.K
Best tax levelSphingobacteriia
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03019,ko03021
Exons1
Model size522
Cds size522
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815719.5023954-CDS-M-pyrifera_M_contig119977:0..5221622815719.5023954-CDS-M-pyrifera_M_contig119977:0..522Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig119977 1..522 -
1692277537.2025206-CDS-M-pyrifera_M_contig119977:0..5221692277537.2025206-CDS-M-pyrifera_M_contig119977:0..522Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig119977 1..522 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig119977.4120.1prot_M-pyrifera_M_contig119977.4120.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig119977 1..522 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig119977.4120.1

>prot_M-pyrifera_M_contig119977.4120.1 ID=prot_M-pyrifera_M_contig119977.4120.1|Name=mRNA_M-pyrifera_M_contig119977.4120.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=174bp
WIGVGGNPGGTGMRVVDLVAPIGKGQRALIVAPPRTGKTVLLKQIANALS
THHKDIHLSVLLVDERPEEVTDFIRSTDAEVFASSNDKKTQSHVRICEMT
LGYAKRKAEMGEDAVLLIDSMTRLGRAYNAMQTNSGRTLSGGLDIRALEI
PKKIFGSARKIEGGGSLTIIATCL
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mRNA from alignment at M-pyrifera_M_contig119977:1..522-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig119977.4120.1 ID=mRNA_M-pyrifera_M_contig119977.4120.1|Name=mRNA_M-pyrifera_M_contig119977.4120.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=522bp|location=Sequence derived from alignment at M-pyrifera_M_contig119977:1..522- (Macrocystis pyrifera P11B4 male)
TGGATTGGGGTGGGGGGTAATCCGGGGGGCACTGGAATGAGAGTGGTTGA
TTTAGTAGCGCCTATTGGTAAAGGTCAGCGTGCTTTAATTGTAGCTCCCC
CAAGAACAGGGAAAACGGTACTGCTAAAGCAAATTGCCAATGCACTTTCG
ACGCACCATAAAGATATTCATTTAAGTGTTCTTCTTGTTGATGAACGACC
TGAGGAGGTAACCGATTTTATTCGATCAACCGATGCTGAGGTATTTGCGT
CGTCAAACGACAAGAAAACACAAAGTCATGTTCGTATTTGTGAAATGACC
CTTGGTTATGCAAAAAGAAAAGCAGAAATGGGTGAAGATGCTGTTCTTCT
TATCGATTCGATGACCCGATTAGGAAGAGCTTACAATGCCATGCAAACGA
ACAGTGGCAGAACTCTTTCAGGAGGCCTTGATATTCGGGCACTTGAAATT
CCTAAAAAAATATTTGGTTCTGCACGCAAAATTGAAGGTGGTGGATCATT
AACTATTATTGCAACCTGCCTT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig119977:1..522-

>mRNA_M-pyrifera_M_contig119977.4120.1 ID=mRNA_M-pyrifera_M_contig119977.4120.1|Name=mRNA_M-pyrifera_M_contig119977.4120.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1044bp|location=Sequence derived from alignment at M-pyrifera_M_contig119977:1..522- (Macrocystis pyrifera P11B4 male)
TGGATTGGGGTGGGGGGTAATCCGGGGGGCACTGGAATGAGAGTGGTTGA
TTTAGTAGCGCCTATTGGTAAAGGTCAGCGTGCTTTAATTGTAGCTCCCC
CAAGAACAGGGAAAACGGTACTGCTAAAGCAAATTGCCAATGCACTTTCG
ACGCACCATAAAGATATTCATTTAAGTGTTCTTCTTGTTGATGAACGACC
TGAGGAGGTAACCGATTTTATTCGATCAACCGATGCTGAGGTATTTGCGT
CGTCAAACGACAAGAAAACACAAAGTCATGTTCGTATTTGTGAAATGACC
CTTGGTTATGCAAAAAGAAAAGCAGAAATGGGTGAAGATGCTGTTCTTCT
TATCGATTCGATGACCCGATTAGGAAGAGCTTACAATGCCATGCAAACGA
ACAGTGGCAGAACTCTTTCAGGAGGCCTTGATATTCGGGCACTTGAAATT
CCTAAAAAAATATTTGGTTCTGCACGCAAAATTGAAGGTGGTGGATCATT
AACTATTATTGCAACCTGCCTTTGGATTGGGGTGGGGGGTAATCCGGGGG
GCACTGGAATGAGAGTGGTTGATTTAGTAGCGCCTATTGGTAAAGGTCAG
CGTGCTTTAATTGTAGCTCCCCCAAGAACAGGGAAAACGGTACTGCTAAA
GCAAATTGCCAATGCACTTTCGACGCACCATAAAGATATTCATTTAAGTG
TTCTTCTTGTTGATGAACGACCTGAGGAGGTAACCGATTTTATTCGATCA
ACCGATGCTGAGGTATTTGCGTCGTCAAACGACAAGAAAACACAAAGTCA
TGTTCGTATTTGTGAAATGACCCTTGGTTATGCAAAAAGAAAAGCAGAAA
TGGGTGAAGATGCTGTTCTTCTTATCGATTCGATGACCCGATTAGGAAGA
GCTTACAATGCCATGCAAACGAACAGTGGCAGAACTCTTTCAGGAGGCCT
TGATATTCGGGCACTTGAAATTCCTAAAAAAATATTTGGTTCTGCACGCA
AAATTGAAGGTGGTGGATCATTAACTATTATTGCAACCTGCCTT
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