mRNA_M-pyrifera_M_contig1199.4111.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig1199.4111.1
Unique NamemRNA_M-pyrifera_M_contig1199.4111.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Match: D8LCF8_ECTSI (Flagellar associated protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LCF8_ECTSI)

HSP 1 Score: 187 bits (475), Expect = 4.120e-56
Identity = 94/109 (86.24%), Postives = 102/109 (93.58%), Query Frame = 1 
Query:    1 ELHEALEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVPPKYKKLTEKGVAGLTSV-SC 324
            +++EALEAPLL VEERLDVLL LKWTV EFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEA+RF+RVPPKY KLT++G+ GLT V SC
Sbjct:  284 DMYEALEAPLLSVEERLDVLLHLKWTVSEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEASRFIRVPPKYGKLTDEGMVGLTGVVSC 392
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BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Match: D8LFI2_ECTSI (MFS domain-containing protein n=2 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LFI2_ECTSI)

HSP 1 Score: 187 bits (475), Expect = 9.940e-55
Identity = 94/109 (86.24%), Postives = 102/109 (93.58%), Query Frame = 1 
Query:    1 ELHEALEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVPPKYKKLTEKGVAGLTSV-SC 324
            +++EALEAPLL VEERLDVLL LKWTV EFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEA+RF+RVPPKY KLT++G+ GLT V SC
Sbjct:  435 DMYEALEAPLLSVEERLDVLLHLKWTVSEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEASRFIRVPPKYGKLTDEGMVGLTGVVSC 543
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BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Match: A0A7S3XKM5_HETAK (Hypothetical protein n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XKM5_HETAK)

HSP 1 Score: 137 bits (346), Expect = 3.490e-38
Identity = 66/90 (73.33%), Postives = 77/90 (85.56%), Query Frame = 1 
Query:    1 ELHEALEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVPP 270
            EL+  L APLLGV+ERLDVLL +KWTV EFDC LTRDI ELVDREADLL+RGRGE +L  LR+R+ NLFLRF ETP+FNPEA RF+++PP
Sbjct:  117 ELYRGLTAPLLGVDERLDVLLHVKWTVKEFDCPLTRDIVELVDREADLLNRGRGEKALEGLRRRVANLFLRFVETPEFNPEAARFLKIPP 206
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BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Match: A0A7S2UXW3_9STRA (Hypothetical protein n=1 Tax=Fibrocapsa japonica TaxID=94617 RepID=A0A7S2UXW3_9STRA)

HSP 1 Score: 126 bits (316), Expect = 1.070e-34
Identity = 61/89 (68.54%), Postives = 73/89 (82.02%), Query Frame = 1 
Query:    1 ELHEALEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVP 267
            EL+E L   L+  +ERLD+LL +KWTV EF+C LTRDI EL+DREADLL+RGR E SL  LRQRI NLFLRF ETP+FNPEATRF+++P
Sbjct:   48 ELYEGLSNSLVSTDERLDILLHVKWTVKEFECPLTRDIVELIDREADLLNRGRSEKSLEGLRQRITNLFLRFIETPEFNPEATRFLKMP 136
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BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Match: A0A7S0PVG3_9EUKA (Hypothetical protein n=1 Tax=Coccolithus braarudii TaxID=221442 RepID=A0A7S0PVG3_9EUKA)

HSP 1 Score: 125 bits (315), Expect = 1.600e-34
Identity = 60/89 (67.42%), Postives = 73/89 (82.02%), Query Frame = 1 
Query:    1 ELHEALEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVP 267
            EL+  L  PLL V+ERLDVLL +KWTV EFDC LTRDI EL+DREAD+L+RGR ++SL  LR+R+ NLFL+F ETP+FNPEA RF +VP
Sbjct:   64 ELYNGLRLPLLSVDERLDVLLHVKWTVKEFDCNLTRDIVELIDREADMLNRGRSDSSLGGLRKRLANLFLQFVETPEFNPEAARFQKVP 152
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BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Match: A0A7S2J4U9_9EUKA (Hypothetical protein n=1 Tax=Haptolina brevifila TaxID=156173 RepID=A0A7S2J4U9_9EUKA)

HSP 1 Score: 126 bits (317), Expect = 3.770e-32
Identity = 60/89 (67.42%), Postives = 73/89 (82.02%), Query Frame = 1 
Query:    1 ELHEALEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVP 267
            EL+  L  PLL ++ERLDVLL +KWTV EFDC LTRD+ EL+DREAD+L+RGR EASL  LR+R+ NLFL+F ETP+FNPEA RF +VP
Sbjct:  385 ELYNGLRLPLLTIDERLDVLLHVKWTVKEFDCNLTRDVVELIDREADMLNRGRSEASLTGLRKRLANLFLQFIETPEFNPEAARFQKVP 473
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BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Match: A0A836CKY2_9STRA (Flagellar associated protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CKY2_9STRA)

HSP 1 Score: 125 bits (313), Expect = 4.980e-32
Identity = 62/107 (57.94%), Postives = 76/107 (71.03%), Query Frame = 1 
Query:    4 LHEALEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVPPKYKKLTEKGVAGLTSVSC 324
            L+ AL APL+G  ERLDVLL +KW V  F+CRLTR++ EL+DREADLLSRGR   S+  LR R   LFLRF ETPDFNPEA+RF+R PP ++ L       L  ++C
Sbjct:  298 LYRALAAPLVGAAERLDVLLHVKWAVRGFECRLTRELVELLDREADLLSRGRAARSMAGLRLRACGLFLRFIETPDFNPEASRFLRAPPSHQHLAVAASKQLVGIAC 404
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BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Match: A0A836CKU2_9STRA (IQ calmodulin-binding motif family protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CKU2_9STRA)

HSP 1 Score: 117 bits (292), Expect = 6.280e-32
Identity = 58/103 (56.31%), Postives = 73/103 (70.87%), Query Frame = 1 
Query:   16 LEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVPPKYKKLTEKGVAGLTSVSC 324
            L APL+G  ERL+VLL++KW V  F+CRLTR++ EL+DREADLLSRGR   S+  LR R   LFLRF ETPDFNPEA+RF+R  P ++ L       L  ++C
Sbjct:    1 LAAPLVGAAERLNVLLRVKWAVRGFECRLTRELVELLDREADLLSRGRAARSMAGLRLRACGLFLRFIETPDFNPEASRFLRAVPSHQHLAVAAAKQLVGIAC 103
[Close]
BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Match: A0A8J8SW50_HALGN (Uncharacterized protein n=2 Tax=Halteria grandinella TaxID=5974 RepID=A0A8J8SW50_HALGN)

HSP 1 Score: 124 bits (311), Expect = 1.400e-31
Identity = 59/97 (60.82%), Postives = 75/97 (77.32%), Query Frame = 1 
Query:    1 ELHEALEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVPPKYKKLTE 291
            +L+  L  P L  +ERLDVLL  KWTV EFDC LTR+I +L+DREAD+L+RGRGEASL  LR+R+ NLFL+F ETP+FNPEA RF +VP +  + T+
Sbjct:  329 DLYNGLRLPFLSTDERLDVLLHTKWTVKEFDCNLTREIVDLIDREADMLNRGRGEASLEGLRKRLSNLFLQFIETPEFNPEAARFQKVPRELLQQTQ 425
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BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Match: A0A7S1GAU3_9STRA (Hypothetical protein n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1GAU3_9STRA)

HSP 1 Score: 122 bits (307), Expect = 2.420e-31
Identity = 59/89 (66.29%), Postives = 72/89 (80.90%), Query Frame = 1 
Query:    1 ELHEALEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLSRGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVP 267
            EL+  L  P+L V+ERLDVLL +KWTV EFDC LTR++ +L+DREADLL+RGR E SL  LR+RI NLFL+F ETP+FNPEA RF +VP
Sbjct:  265 ELYSGLNLPMLTVDERLDVLLHVKWTVKEFDCLLTREVVDLIDREADLLNRGRSEKSLEGLRRRISNLFLQFIETPEFNPEAARFQKVP 353
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig1199.4111.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LCF8_ECTSI4.120e-5686.24Flagellar associated protein n=1 Tax=Ectocarpus si... [more]
D8LFI2_ECTSI9.940e-5586.24MFS domain-containing protein n=2 Tax=Ectocarpus s... [more]
A0A7S3XKM5_HETAK3.490e-3873.33Hypothetical protein n=1 Tax=Heterosigma akashiwo ... [more]
A0A7S2UXW3_9STRA1.070e-3468.54Hypothetical protein n=1 Tax=Fibrocapsa japonica T... [more]
A0A7S0PVG3_9EUKA1.600e-3467.42Hypothetical protein n=1 Tax=Coccolithus braarudii... [more]
A0A7S2J4U9_9EUKA3.770e-3267.42Hypothetical protein n=1 Tax=Haptolina brevifila T... [more]
A0A836CKY2_9STRA4.980e-3257.94Flagellar associated protein n=1 Tax=Tribonema min... [more]
A0A836CKU2_9STRA6.280e-3256.31IQ calmodulin-binding motif family protein n=1 Tax... [more]
A0A8J8SW50_HALGN1.400e-3160.82Uncharacterized protein n=2 Tax=Halteria grandinel... [more]
A0A7S1GAU3_9STRA2.420e-3166.29Hypothetical protein n=1 Tax=Bicosoecida sp. CB-20... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig1199contigM-pyrifera_M_contig1199:27571..27897 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score187.2
Seed ortholog evalue4.1e-45
Seed eggNOG ortholog2880.D8LCF8
Preferred nameIQUB
Hectar predicted targeting categoryother localisation
GOsGO:0001669,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005929,GO:0006996,GO:0007154,GO:0007165,GO:0007166,GO:0007224,GO:0008150,GO:0009987,GO:0012505,GO:0016043,GO:0022607,GO:0023052,GO:0030030,GO:0030031,GO:0030141,GO:0031410,GO:0031514,GO:0031982,GO:0042995,GO:0043226,GO:0043227,GO:0043229,GO:0044085,GO:0044424,GO:0044444,GO:0044464,GO:0044782,GO:0050789,GO:0050794,GO:0050896,GO:0051716,GO:0060271,GO:0065007,GO:0070925,GO:0071840,GO:0097223,GO:0097708,GO:0099503,GO:0120025,GO:0120031,GO:0120036
EggNOG free text desc.smoothened signaling pathway
EggNOG OGs28JBT@1,2QRQT@2759
Ec32 ortholog descriptionIQ calmodulin-binding motif family protein
Ec32 orthologEc-00_007120.1
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size327
Cds size327
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815719.0583441-CDS-M-pyrifera_M_contig1199:27570..278971622815719.0583441-CDS-M-pyrifera_M_contig1199:27570..27897Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig1199 27571..27897 +
1692277536.981912-CDS-M-pyrifera_M_contig1199:27570..278971692277536.981912-CDS-M-pyrifera_M_contig1199:27570..27897Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig1199 27571..27897 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig1199.4111.1prot_M-pyrifera_M_contig1199.4111.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig1199 27571..27897 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig1199.4111.1

>prot_M-pyrifera_M_contig1199.4111.1 ID=prot_M-pyrifera_M_contig1199.4111.1|Name=mRNA_M-pyrifera_M_contig1199.4111.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=109bp
ELHEALEAPLLGVEERLDVLLQLKWTVGEFDCRLTRDIAELVDREADLLS
RGRGEASLRPLRQRIRNLFLRFAETPDFNPEATRFVRVPPKYKKLTEKGV
AGLTSVSC*
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mRNA from alignment at M-pyrifera_M_contig1199:27571..27897+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig1199.4111.1 ID=mRNA_M-pyrifera_M_contig1199.4111.1|Name=mRNA_M-pyrifera_M_contig1199.4111.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=327bp|location=Sequence derived from alignment at M-pyrifera_M_contig1199:27571..27897+ (Macrocystis pyrifera P11B4 male)
GAGTTACACGAGGCCCTCGAAGCCCCGCTGCTGGGCGTAGAAGAACGCCT
CGACGTGCTACTGCAGCTGAAGTGGACCGTCGGCGAGTTCGACTGCCGGC
TCACCCGAGACATAGCGGAGCTTGTAGATAGGGAGGCGGACCTTCTTAGC
CGGGGGAGGGGGGAGGCTAGCCTGCGTCCCCTGCGCCAGCGCATCCGCAA
CCTATTCCTTCGGTTTGCGGAAACCCCTGACTTCAACCCGGAGGCGACGC
GGTTCGTGCGCGTACCGCCCAAGTACAAGAAGCTCACGGAGAAGGGCGTG
GCGGGGTTGACATCTGTCTCGTGTTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig1199:27571..27897+

>mRNA_M-pyrifera_M_contig1199.4111.1 ID=mRNA_M-pyrifera_M_contig1199.4111.1|Name=mRNA_M-pyrifera_M_contig1199.4111.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=654bp|location=Sequence derived from alignment at M-pyrifera_M_contig1199:27571..27897+ (Macrocystis pyrifera P11B4 male)
GAGTTACACGAGGCCCTCGAAGCCCCGCTGCTGGGCGTAGAAGAACGCCT
CGACGTGCTACTGCAGCTGAAGTGGACCGTCGGCGAGTTCGACTGCCGGC
TCACCCGAGACATAGCGGAGCTTGTAGATAGGGAGGCGGACCTTCTTAGC
CGGGGGAGGGGGGAGGCTAGCCTGCGTCCCCTGCGCCAGCGCATCCGCAA
CCTATTCCTTCGGTTTGCGGAAACCCCTGACTTCAACCCGGAGGCGACGC
GGTTCGTGCGCGTACCGCCCAAGTACAAGAAGCTCACGGAGAAGGGCGTG
GCGGGGTTGACATCTGTCTCGTGTTGAGAGTTACACGAGGCCCTCGAAGC
CCCGCTGCTGGGCGTAGAAGAACGCCTCGACGTGCTACTGCAGCTGAAGT
GGACCGTCGGCGAGTTCGACTGCCGGCTCACCCGAGACATAGCGGAGCTT
GTAGATAGGGAGGCGGACCTTCTTAGCCGGGGGAGGGGGGAGGCTAGCCT
GCGTCCCCTGCGCCAGCGCATCCGCAACCTATTCCTTCGGTTTGCGGAAA
CCCCTGACTTCAACCCGGAGGCGACGCGGTTCGTGCGCGTACCGCCCAAG
TACAAGAAGCTCACGGAGAAGGGCGTGGCGGGGTTGACATCTGTCTCGTG
TTGA
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