mRNA_M-pyrifera_M_contig119268.3966.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview


Name	mRNA_M-pyrifera_M_contig119268.3966.1
Unique Name	mRNA_M-pyrifera_M_contig119268.3966.1
Type	mRNA
Organism	Macrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))

Homology

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BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Match: A0A6J8A148_MYTCO (Methyltranfer_dom domain-containing protein n=2 Tax=Mytilus coruscus TaxID=42192 RepID=A0A6J8A148_MYTCO)

HSP 1 Score: 78.2 bits (191), Expect = 3.830e-14
Identity = 44/147 (29.93%), Postives = 76/147 (51.70%), Query Frame = 1

Query:   34 VERWLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQPLHELTLQECSDLAFGLRKAEWPQSLRDFLDRCDRSTMPTAEEVEELRMEVDVRLRGMKGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYLCHSL 474
            +ER L++ +  + EF W+    LT F+ +  +E      P EW + L  L+ +  + L FG     WP+S R+FL +    +  ++   + L   V++     +GM  KK HEV  +  LV  +   +G+  +VD+G+G+GYL  +L
Sbjct:    1 MERQLKQMVDFLHEFDWIYNFKLTDFFIDEVWEN----IPGEWAECLLSLSNEHLNQLPFGFMVDTWPESFREFLQKA--ISFSSSRNCKSLEKSVEIDPNIKRGMNPKKQHEVSKMAALVHDLIKDSGSDIIVDVGSGLGYLGQTL 141

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BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Match: A0A1D1UJV1_RAMVA (Methyltranfer_dom domain-containing protein n=1 Tax=Ramazzottius varieornatus TaxID=947166 RepID=A0A1D1UJV1_RAMVA)

HSP 1 Score: 72.8 bits (177), Expect = 4.430e-12
Identity = 55/161 (34.16%), Postives = 82/161 (50.93%), Query Frame = 1

Query:   31 EVERWLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQPLHELTLQECSDLAFGLR-------KAEWPQSLRDFL----DRCDRSTM------PTAEEVEELRMEVDVRLRGMKGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYL 462
            E + +LE +L  +  ++WL +  +T FY +  +E      P+EW Q    L+ +E       LR       +A +P+SLR FL    D C +  M        A E+E    EVD+  R M GM+ KKLHEV  +  L+  +    G +TV+D+GAG+GYL
Sbjct:   13 EEDAYLERALGFIIRYKWLHDFKITRFYVDNIHEN----LPQEWVQASSGLSTEELQTQLDVLRNSSSTEVRAGFPESLRMFLREAKDLCLKRAMLGEGKGGGALELES-EHEVDIPRRLMYGMRPKKLHEVSRMAVLIHQICQKYGLRTVLDLGAGMGYL 168

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BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Match: A0A026W112_OOCBI (Protein RRNAD1 n=1 Tax=Ooceraea biroi TaxID=2015173 RepID=A0A026W112_OOCBI)

HSP 1 Score: 72.0 bits (175), Expect = 6.940e-12
Identity = 46/147 (31.29%), Postives = 72/147 (48.98%), Query Frame = 1

Query:   40 RWLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQPLHELTLQECSDLAFGLR-KAEWPQSLRDFLDRCDR-STMPTAEEVEELRMEVDVRLRGMKGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYLCHSL 474
            ++  E+L L  E QWL    +T      + +      PK W + L  L  +E ++ A G   KAEWP SL+ +L RC   + +P    +  + +  + R     G+ +KK HE+  L  LV         QT++D+GAG+GY+C  L
Sbjct:   11 KYFFEALCLFHEMQWLHNVPVTEILTRRSLDA----IPKGWTEQLEILKNEELNNFAMGKTIKAEWPDSLKAYLSRCKEINRLPLVPVLSSVELPKNFRT----GLSQKKQHEIMHLANLVHEQCTPHNIQTIIDLGAGLGYVCQML 149

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BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Match: A0A3L8DT38_OOCBI (Methyltranfer_dom domain-containing protein n=1 Tax=Ooceraea biroi TaxID=2015173 RepID=A0A3L8DT38_OOCBI)

HSP 1 Score: 72.0 bits (175), Expect = 9.050e-12
Identity = 46/147 (31.29%), Postives = 72/147 (48.98%), Query Frame = 1

Query:   40 RWLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQPLHELTLQECSDLAFGLR-KAEWPQSLRDFLDRCDR-STMPTAEEVEELRMEVDVRLRGMKGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYLCHSL 474
            ++  E+L L  E QWL    +T      + +      PK W + L  L  +E ++ A G   KAEWP SL+ +L RC   + +P    +  + +  + R     G+ +KK HE+  L  LV         QT++D+GAG+GY+C  L
Sbjct:   11 KYFFEALCLFHEMQWLHNVPVTEILTRRSLDA----IPKGWTEQLEILKNEELNNFAMGKTIKAEWPDSLKAYLSRCKEINRLPLVPVLSSVELPKNFRT----GLSQKKQHEIMHLANLVHEQCTPHNIQTIIDLGAGLGYVCQML 149

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BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Match: A0A1U8Q4G1_NELNU (methyltransferase-like protein 25 isoform X7 n=1 Tax=Nelumbo nucifera TaxID=4432 RepID=A0A1U8Q4G1_NELNU)

HSP 1 Score: 71.6 bits (174), Expect = 1.200e-11
Identity = 44/149 (29.53%), Postives = 77/149 (51.68%), Query Frame = 1

Query:   43 WLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQPLHELTLQECSDLAFGLRKAEWPQSLRDFLDRCDRSTMPTAEEVEELRMEVDVRLRGM-----KGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYLCHSL 474
            W+      +  + +LL   +  F+ +  +E       K+W   L + +++    +  G+ +  WP SL++F+    RS +   E+VE   M  ++ +  +     +GM  KK HEVE L  +V ++A STG+QT++D+GAG GYL   L
Sbjct:   18 WISAINDFLRPYMFLLNDHVVNFFKDRLWES----IDKQWMNCLRKESVENLLKIPSGIVQDNWPASLKEFI-LTSRSLVLPREQVELQMMLPNLHVASLDTVLTQGMNMKKKHEVETLAAVVRSIAESTGSQTIIDVGAGQGYLAQVL 161

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BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Match: A0A1U8Q613_NELNU (methyltransferase-like protein 25 isoform X5 n=2 Tax=Nelumbo nucifera TaxID=4432 RepID=A0A1U8Q613_NELNU)

HSP 1 Score: 71.6 bits (174), Expect = 1.230e-11
Identity = 44/149 (29.53%), Postives = 77/149 (51.68%), Query Frame = 1

Query:   43 WLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQPLHELTLQECSDLAFGLRKAEWPQSLRDFLDRCDRSTMPTAEEVEELRMEVDVRLRGM-----KGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYLCHSL 474
            W+      +  + +LL   +  F+ +  +E       K+W   L + +++    +  G+ +  WP SL++F+    RS +   E+VE   M  ++ +  +     +GM  KK HEVE L  +V ++A STG+QT++D+GAG GYL   L
Sbjct:   18 WISAINDFLRPYMFLLNDHVVNFFKDRLWES----IDKQWMNCLRKESVENLLKIPSGIVQDNWPASLKEFI-LTSRSLVLPREQVELQMMLPNLHVASLDTVLTQGMNMKKKHEVETLAAVVRSIAESTGSQTIIDVGAGQGYLAQVL 161

[Close]

BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Match: A0A1U7ZW38_NELNU (protein RRNAD1 isoform X1 n=4 Tax=Nelumbo nucifera TaxID=4432 RepID=A0A1U7ZW38_NELNU)

HSP 1 Score: 71.6 bits (174), Expect = 1.320e-11
Identity = 44/149 (29.53%), Postives = 77/149 (51.68%), Query Frame = 1

Query:   43 WLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQPLHELTLQECSDLAFGLRKAEWPQSLRDFLDRCDRSTMPTAEEVEELRMEVDVRLRGM-----KGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYLCHSL 474
            W+      +  + +LL   +  F+ +  +E       K+W   L + +++    +  G+ +  WP SL++F+    RS +   E+VE   M  ++ +  +     +GM  KK HEVE L  +V ++A STG+QT++D+GAG GYL   L
Sbjct:   18 WISAINDFLRPYMFLLNDHVVNFFKDRLWES----IDKQWMNCLRKESVENLLKIPSGIVQDNWPASLKEFI-LTSRSLVLPREQVELQMMLPNLHVASLDTVLTQGMNMKKKHEVETLAAVVRSIAESTGSQTIIDVGAGQGYLAQVL 161

[Close]

BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Match: A0A0J7L1X3_LASNI (Protein rrnad1-like protein n=1 Tax=Lasius niger TaxID=67767 RepID=A0A0J7L1X3_LASNI)

HSP 1 Score: 70.9 bits (172), Expect = 1.670e-11
Identity = 45/146 (30.82%), Postives = 76/146 (52.05%), Query Frame = 1

Query:   40 RWLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQPLHELTLQECSDL-AFGLRKAEWPQSLRDFLDRCDRSTMPTAEEVEELRMEVDVRLRGMKGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYLCHSL 474
            ++  E+L+L+ E QWL   ++T    + + +      PKEW + L  L  +E ++L A    K+EWP SL+ ++++C         +    R+E+  + +   G+  KK HE+  L  LV         QT+VD+GAG+GY+C  L
Sbjct:    8 KYFFEALSLLYEMQWLHNIAVTEILTKASLDA----IPKEWIEHLRILENEELNNLIARRTIKSEWPDSLKTYVEKCQNINRLPLVQTSPFRIELPQKFKI--GLSRKKQHEIIHLAHLVHTQCKLHDIQTIVDLGAGLGYVCQML 147

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BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Match: A0A7N1A434_KALFE (Methyltranfer_dom domain-containing protein n=1 Tax=Kalanchoe fedtschenkoi TaxID=63787 RepID=A0A7N1A434_KALFE)

HSP 1 Score: 70.9 bits (172), Expect = 2.260e-11
Identity = 44/148 (29.73%), Postives = 76/148 (51.35%), Query Frame = 1

Query:   43 WLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQPLHELTLQECSDLAFGLRKAEWPQSLRDFLDRCDRSTMPTAE-EVEELRMEVDVRLRG---MKGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYLCHSL 474
            W+E  +  +  + +L    +  F+ +  +E       K+W   L    +Q    +  G+ +A WP SL++F+      ++P  +  +E++   V +   G    +GM  KK HEVEVL+ +V+++A S   QT+VD+GAG GYL   L
Sbjct:   16 WIEAIVGFLKPYTFLSNAHVVNFFKDKLWEA----LDKDWISCLSREPIQNLLLIPSGIVQAHWPASLKEFVSTVRSLSLPQRQANLEKILPNVHITSLGPVLAQGMNVKKKHEVEVLSAIVDSIARSVKAQTIVDVGAGQGYLSQVL 159

[Close]

BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Match: A0A7N1A231_KALFE (Methyltranfer_dom domain-containing protein n=1 Tax=Kalanchoe fedtschenkoi TaxID=63787 RepID=A0A7N1A231_KALFE)

HSP 1 Score: 70.9 bits (172), Expect = 2.420e-11
Identity = 44/148 (29.73%), Postives = 76/148 (51.35%), Query Frame = 1

Query:   43 WLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQPLHELTLQECSDLAFGLRKAEWPQSLRDFLDRCDRSTMPTAE-EVEELRMEVDVRLRG---MKGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYLCHSL 474
            W+E  +  +  + +L    +  F+ +  +E       K+W   L    +Q    +  G+ +A WP SL++F+      ++P  +  +E++   V +   G    +GM  KK HEVEVL+ +V+++A S   QT+VD+GAG GYL   L
Sbjct:   16 WIEAIVGFLKPYTFLSNAHVVNFFKDKLWEA----LDKDWISCLSREPIQNLLLIPSGIVQAHWPASLKEFVSTVRSLSLPQRQANLEKILPNVHITSLGPVLAQGMNVKKKHEVEVLSAIVDSIARSVKAQTIVDVGAGQGYLSQVL 159

The following BLAST results are available for this feature:

BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot

BLAST of mRNA_M-pyrifera_M_contig119268.3966.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25


Match Name	E-value	Identity	Description
A0A6J8A148_MYTCO	3.830e-14	29.93	Methyltranfer_dom domain-containing protein n=2 Ta...	[more]
A0A1D1UJV1_RAMVA	4.430e-12	34.16	Methyltranfer_dom domain-containing protein n=1 Ta...	[more]
A0A026W112_OOCBI	6.940e-12	31.29	Protein RRNAD1 n=1 Tax=Ooceraea biroi TaxID=201517...	[more]
A0A3L8DT38_OOCBI	9.050e-12	31.29	Methyltranfer_dom domain-containing protein n=1 Ta...	[more]
A0A1U8Q4G1_NELNU	1.200e-11	29.53	methyltransferase-like protein 25 isoform X7 n=1 T...	[more]
A0A1U8Q613_NELNU	1.230e-11	29.53	methyltransferase-like protein 25 isoform X5 n=2 T...	[more]
A0A1U7ZW38_NELNU	1.320e-11	29.53	protein RRNAD1 isoform X1 n=4 Tax=Nelumbo nucifera...	[more]
A0A0J7L1X3_LASNI	1.670e-11	30.82	Protein rrnad1-like protein n=1 Tax=Lasius niger T...	[more]
A0A7N1A434_KALFE	2.260e-11	29.73	Methyltranfer_dom domain-containing protein n=1 Ta...	[more]
A0A7N1A231_KALFE	2.420e-11	29.73	Methyltranfer_dom domain-containing protein n=1 Ta...	[more]

Pages

Alignments

The following features are aligned


Aligned Feature	Feature Type	Alignment Location
M-pyrifera_M_contig119268	contig	M-pyrifera_M_contig119268:14..541 +

Analyses

This mRNA is derived from or has results from the following analyses


Analysis Name	Date Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90	2022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male	2021-02-24

Properties


Property Name	Value
Taxonomic scope	Metazoa
Seed ortholog score	82.0
Seed ortholog evalue	2.6e-13
Seed eggNOG ortholog	10228.TriadP60323
Hectar predicted targeting category	other localisation
EggNOG free text desc.	Ribosomal RNA adenine dimethylase domain containing 1
EggNOG OGs	395PJ@33154,3BB1H@33208,KOG2651@1,KOG2651@2759
COG Functional cat.	A
Best tax level	Metazoa
Best eggNOG OG	NA\|NA\|NA
Exons	2
Model size	474
Cds size	453
Stop	0
Start	1

Relationships

The following polypeptide feature(s) derives from this mRNA:


Feature Name	Unique Name	Species	Type	Position
mRNA_M-pyrifera_M_contig119268.3966.1	prot_M-pyrifera_M_contig119268.3966.1	Macrocystis pyrifera P11B4 male	polypeptide	M-pyrifera_M_contig119268 35..541 +

The following UTR feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1622815711.9030113-UTR-M-pyrifera_M_contig119268:13..34	1622815711.9030113-UTR-M-pyrifera_M_contig119268:13..34	Macrocystis pyrifera P11B4 male	UTR	M-pyrifera_M_contig119268 14..34 +
1692277533.9387643-UTR-M-pyrifera_M_contig119268:13..34	1692277533.9387643-UTR-M-pyrifera_M_contig119268:13..34	Macrocystis pyrifera P11B4 male	UTR	M-pyrifera_M_contig119268 14..34 +

The following CDS feature(s) are a part of this mRNA:


Feature Name	Unique Name	Species	Type	Position
1622815711.9150534-CDS-M-pyrifera_M_contig119268:34..287	1622815711.9150534-CDS-M-pyrifera_M_contig119268:34..287	Macrocystis pyrifera P11B4 male	CDS	M-pyrifera_M_contig119268 35..287 +
1692277533.9509752-CDS-M-pyrifera_M_contig119268:34..287	1692277533.9509752-CDS-M-pyrifera_M_contig119268:34..287	Macrocystis pyrifera P11B4 male	CDS	M-pyrifera_M_contig119268 35..287 +
1622815711.9288535-CDS-M-pyrifera_M_contig119268:341..541	1622815711.9288535-CDS-M-pyrifera_M_contig119268:341..541	Macrocystis pyrifera P11B4 male	CDS	M-pyrifera_M_contig119268 342..541 +
1692277533.9610288-CDS-M-pyrifera_M_contig119268:341..541	1692277533.9610288-CDS-M-pyrifera_M_contig119268:341..541	Macrocystis pyrifera P11B4 male	CDS	M-pyrifera_M_contig119268 342..541 +

Sequences

The following sequences are available for this feature:

protein sequence
mRNA from alignment at M-pyrifera_M_contig119268:14..541+
coding sequence from alignment at M-pyrifera_M_contig119268:14..541+

protein sequence of mRNA_M-pyrifera_M_contig119268.3966.1

>prot_M-pyrifera_M_contig119268.3966.1 ID=prot_M-pyrifera_M_contig119268.3966.1|Name=mRNA_M-pyrifera_M_contig119268.3966.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=151bp
MPEEVERWLEESLALVTEFQWLLEQSLTGFYAEGAYERDSWPFPKEWRQP
LHELTLQECSDLAFGLRKAEWPQSLRDFLDRCDRSTMPTAEEVEELRMEV
DVRLRGMKGMKEKKLHEVEVLTGLVEAVAGSTGTQTVVDIGAGVGYLCHS
L

mRNA from alignment at M-pyrifera_M_contig119268:14..541+

Legend: UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

>mRNA_M-pyrifera_M_contig119268.3966.1 ID=mRNA_M-pyrifera_M_contig119268.3966.1|Name=mRNA_M-pyrifera_M_contig119268.3966.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=528bp|location=Sequence derived from alignment at M-pyrifera_M_contig119268:14..541+ (Macrocystis pyrifera P11B4 male)
ATTGCTCTCACTTCCTCTGTGATGCCTGAGGAGGTGGAGCGATGGTTGGA
GGAGAGCCTTGCCCTTGTGACCGAGTTCCAGTGGTTGCTCGAACAGTCAC
TGACAGGATTCTATGCAGAAGGGGCGTATGAGAGAGACTCATGGCCCTTT
CCAAAGGAGTGGAGGCAGCCACTGCACGAGCTCACCCTCCAGGAGTGCAG
CGACCTTGCCTTTGGCCTGAGGAAAGCAGAATGGCCTCAGTCTCTCAGAG
ACTTCTTGGACAGATGTGACAGGTGTTCACTTCCTCGCAGACTCCAAGAA
GAGAAGGAAATAGAGACCTCTACGCGAGCTACGATGCCCACGGCTGAAGA
GGTCGAGGAGCTGAGGATGGAGGTCGACGTACGCCTACGTGGCATGAAGG
GCATGAAAGAGAAGAAGCTGCATGAGGTGGAAGTGCTCACTGGTCTAGTG
GAGGCCGTTGCTGGCTCCACTGGCACACAGACAGTCGTGGACATAGGGGC
AGGAGTGGGCTACCTCTGTCACTCTCTG

Coding sequence (CDS) from alignment at M-pyrifera_M_contig119268:14..541+

>mRNA_M-pyrifera_M_contig119268.3966.1 ID=mRNA_M-pyrifera_M_contig119268.3966.1|Name=mRNA_M-pyrifera_M_contig119268.3966.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=906bp|location=Sequence derived from alignment at M-pyrifera_M_contig119268:14..541+ (Macrocystis pyrifera P11B4 male)
ATGCCTGAGGAGGTGGAGCGATGGTTGGAGGAGAGCCTTGCCCTTGTGAC
CGAGTTCCAGTGGTTGCTCGAACAGTCACTGACAGGATTCTATGCAGAAG
GGGCGTATGAGAGAGACTCATGGCCCTTTCCAAAGGAGTGGAGGCAGCCA
CTGCACGAGCTCACCCTCCAGGAGTGCAGCGACCTTGCCTTTGGCCTGAG
GAAAGCAGAATGGCCTCAGTCTCTCAGAGACTTCTTGGACAGATGTGACA
GGTATGCCTGAGGAGGTGGAGCGATGGTTGGAGGAGAGCCTTGCCCTTGT
GACCGAGTTCCAGTGGTTGCTCGAACAGTCACTGACAGGATTCTATGCAG
AAGGGGCGTATGAGAGAGACTCATGGCCCTTTCCAAAGGAGTGGAGGCAG
CCACTGCACGAGCTCACCCTCCAGGAGTGCAGCGACCTTGCCTTTGGCCT
GAGGAAAGCAGAATGGCCTCAGTCTCTCAGAGACTTCTTGGACAGATGTG
ACAGGTCTACGATGCCCACGGCTGAAGAGGTCGAGGAGCTGAGGATGGAG
GTCGACGTACGCCTACGTGGCATGAAGGGCATGAAAGAGAAGAAGCTGCA
TGAGGTGGAAGTGCTCACTGGTCTAGTGGAGGCCGTTGCTGGCTCCACTG
GCACACAGACAGTCGTGGACATAGGGGCAGGAGTGGGCTACCTCTGTCAC
TCTCTGCTACGATGCCCACGGCTGAAGAGGTCGAGGAGCTGAGGATGGAG
GTCGACGTACGCCTACGTGGCATGAAGGGCATGAAAGAGAAGAAGCTGCA
TGAGGTGGAAGTGCTCACTGGTCTAGTGGAGGCCGTTGCTGGCTCCACTG
GCACACAGACAGTCGTGGACATAGGGGCAGGAGTGGGCTACCTCTGTCAC
TCTCTG