mRNA_M-pyrifera_M_contig114353.2997.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig114353.2997.1
Unique NamemRNA_M-pyrifera_M_contig114353.2997.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Match: A0A3C0HSC0_9ACTN (Ribonuclease PH n=6 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A3C0HSC0_9ACTN)

HSP 1 Score: 297 bits (761), Expect = 4.310e-100
Identity = 153/175 (87.43%), Postives = 158/175 (90.29%), Query Frame = 1 
Query:    1 PERVNRERRGAKGRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQADGGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPVLDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAEGGIATITAAQRETLATAPSPRSPGR 525
            PER+NRERRGAKGRTQEIERLIGRSLRAVTDMRALGE SIRLDCDVLQADGGTRTASICGA+VALHDACERLVQ G I  NPL TEC AISVGIVDG PVLDLPYPEDSTADVDFNVIM G GQLIEVQGTAEGNTF RAELD LLDLAEGG+ATI  AQR  LA AP+PR+PGR
Sbjct:   75 PERINRERRGAKGRTQEIERLIGRSLRAVTDMRALGETSIRLDCDVLQADGGTRTASICGAYVALHDACERLVQAGHIPANPLTTECVAISVGIVDGRPVLDLPYPEDSTADVDFNVIMDGNGQLIEVQGTAEGNTFSRAELDVLLDLAEGGVATIAEAQRAVLADAPAPRTPGR 249
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BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Match: A0A5Q2RLF6_9ACTN (Ribonuclease PH n=1 Tax=Actinomarinicola tropica TaxID=2789776 RepID=A0A5Q2RLF6_9ACTN)

HSP 1 Score: 236 bits (603), Expect = 4.650e-76
Identity = 124/173 (71.68%), Postives = 137/173 (79.19%), Query Frame = 1 
Query:    1 PERVNRERRGAK--GRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQADGGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPVLDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAEGGIATITAAQRETLATAPSPR 513
            PER+ RE +  K  GRTQEI+RLIGR+LRAVTDM ALGE  I LDCDVLQADGGTRTASICGA+VALHDAC RLV  G +A NP+   CAAISVGI+DG P LDLPY EDS A+VD NV+MTG G  +EVQGTAEG  + RAELDALL LAEGGIATI   QRE +A AP PR
Sbjct:   78 PERIRREVKDGKPSGRTQEIQRLIGRALRAVTDMEALGERQILLDCDVLQADGGTRTASICGAWVALHDACSRLVATGRLAANPITDACAAISVGIIDGQPRLDLPYEEDSRAEVDMNVVMTGAGGFVEVQGTAEGAPYSRAELDALLGLAEGGIATIVGMQREVVAAAPDPR 250
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BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Match: A0A7X6ART5_9ACTN (Ribonuclease PH (Fragment) n=1 Tax=Actinomycetia bacterium TaxID=1883427 RepID=A0A7X6ART5_9ACTN)

HSP 1 Score: 238 bits (606), Expect = 5.620e-76
Identity = 123/165 (74.55%), Postives = 136/165 (82.42%), Query Frame = 1 
Query:    1 PERVNRERRGAKGRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQADGGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPVLDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAEGGIATITAAQRETLA 495
            PERV R R G  GR+QEI+RLIGRSLRAVTD++ALGE SI +DCDVLQADGGTRTASICGA+VALHDAC RLVQ G +  +PLVTECAAISVGIVDG P+LDLPY ED  ADVDFNV+MTGTG LIEVQGTAEG  F R +LD +LDLA  GI+ I  AQR  LA
Sbjct:   74 PERVRRPRTGPNGRSQEIQRLIGRSLRAVTDLKALGEVSIDIDCDVLQADGGTRTASICGAYVALHDACSRLVQAGLMEADPLVTECAAISVGIVDGMPMLDLPYREDVAADVDFNVVMTGTGNLIEVQGTAEGEPFSREQLDQILDLAAKGISEINEAQRAVLA 238
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BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Match: A0A6B1AVU3_9ACTN (Ribonuclease PH n=1 Tax=Acidimicrobiaceae bacterium TaxID=2024894 RepID=A0A6B1AVU3_9ACTN)

HSP 1 Score: 231 bits (590), Expect = 2.790e-74
Identity = 124/172 (72.09%), Postives = 132/172 (76.74%), Query Frame = 1 
Query:    1 PERVNRERRGAKGRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQADGGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPVLDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAEGGIATITAAQRETLATAPSPRS 516
            PER+   RR   GR QEI+RLIGRSLR V DM ALGE SI +DCDVL ADGGTRTASICG +VALHDACERLV  G I  NPL TECAAISVGIVDG P+LDLPY EDSTADVDFNV+MTG G LIEVQGTAEG  F R EL A+LDLA  GI  I  AQR  L T P PR+
Sbjct:   65 PERI---RRRTSGRDQEIQRLIGRSLRCVIDMAALGERSITVDCDVLSADGGTRTASICGGYVALHDACERLVLAGQIPGNPLFTECAAISVGIVDGRPLLDLPYLEDSTADVDFNVVMTGDGSLIEVQGTAEGKAFSRDELTAMLDLAAKGITEINEAQRAVLRTPPEPRA 233
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BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Match: A0A3S0DTR6_9ACTN (Ribonuclease PH n=2 Tax=Acidimicrobiia TaxID=84992 RepID=A0A3S0DTR6_9ACTN)

HSP 1 Score: 226 bits (575), Expect = 7.050e-72
Identity = 118/175 (67.43%), Postives = 138/175 (78.86%), Query Frame = 1 
Query:    1 PERVNRERRGAKGRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQADGGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPVLDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAEGGIATITAAQRETLATAPSPRSPGR 525
            PER+ RE +G KGRTQEI+RLIGR+LR+V DM+ALGE  I +DCDVLQADGGTRTASI GA++ALHD   R+VQ G +++ PL  E AAISVG+VDG PVLDLPY EDSTA+ D NV+MTG G  +EVQGTAEG  F R ELD+LL LAEGGI  I A QR+ LA AP+PR  GR
Sbjct:   71 PERIRRETKGPKGRTQEIQRLIGRALRSVCDMKALGERQILIDCDVLQADGGTRTASISGAYLALHDCLTRVVQAGLLSEVPLYDELAAISVGVVDGVPVLDLPYTEDSTAETDMNVVMTGKGLFVEVQGTAEGAPFSRDELDSLLGLAEGGIRRIHALQRKYLAEAPTPRPIGR 245
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BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Match: A0A7Y2GKH5_9ACTN (Ribonuclease PH n=1 Tax=Acidimicrobiales bacterium TaxID=2201156 RepID=A0A7Y2GKH5_9ACTN)

HSP 1 Score: 225 bits (573), Expect = 1.170e-71
Identity = 118/171 (69.01%), Postives = 136/171 (79.53%), Query Frame = 1 
Query:    1 PERVNRERRGAKGRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQADGGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPVLDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAEGGIATITAAQRETLATAPSPR 513
            PERV   RR   GRTQEI+RLIGRSLR+V D  A+GE S+ +DCDVLQADGGTRTASICG +VALHDA ERL+QGG +  NP++TECAAISVGIVDG P+LDLPY ED+ A+VD NV++ G+G  IEVQGTAEG TF RAELDALL LAE G+A I  AQR+ L T P  R
Sbjct:   71 PERV---RRRVSGRTQEIQRLIGRSLRSVVDFEAMGEVSVIVDCDVLQADGGTRTASICGGYVALHDALERLIQGGQLGANPIMTECAAISVGIVDGEPLLDLPYIEDANAEVDMNVVIVGSGAYIEVQGTAEGATFSRAELDALLGLAEKGVAEIIDAQRDLLQTPPELR 238
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BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Match: A0A2E7NHQ5_9BACT (Ribonuclease PH n=6 Tax=root TaxID=1 RepID=A0A2E7NHQ5_9BACT)

HSP 1 Score: 219 bits (557), Expect = 3.140e-69
Identity = 111/165 (67.27%), Postives = 130/165 (78.79%), Query Frame = 1 
Query:   22 RRGAKGRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQADGGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPVLDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAEGGIATITAAQRETLATAPSPRS 516
            RR   GR+QEI+RLI RSLRAV D+  LGE  + +DCDV+QADGGTRTASICG F+ALHDA ERLV  G +A NPL  + AA+SVGIV+G P LDLPY EDSTA+VDFNV+MTG+G+ +EVQGTAEG  FDRA+LDAL+DLA GGIA I  AQR  LA  P  R+
Sbjct:   75 RRRVSGRSQEIQRLIARSLRAVCDLEVLGEREVTVDCDVIQADGGTRTASICGGFIALHDALERLVVSGDLAANPLTDQLAAVSVGIVEGEPRLDLPYVEDSTAEVDFNVVMTGSGRFVEVQGTAEGEAFDRAQLDALVDLATGGIAEIAEAQRALLADPPPART 239
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BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Match: A0A2E9XE16_9ACTN (Ribonuclease PH n=3 Tax=Actinobacteria TaxID=201174 RepID=A0A2E9XE16_9ACTN)

HSP 1 Score: 218 bits (556), Expect = 4.590e-69
Identity = 115/167 (68.86%), Postives = 132/167 (79.04%), Query Frame = 1 
Query:    4 ERVNRERRGAK--GRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQADGGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPVLDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAEGGIATITAAQRETLAT 498
            ERVNRE +  K  GRT EIERLIGRSLRA+ DM+ LGE  + +DCDVLQADGGTRTASICG FVALHDAC  L++ G I  +PL   CAAISVGIVDG+P LDLPY EDSTA VD NV+MTG+G+ +EVQGTAEG+ FDR+ELDAL  LAE GI+ I A Q+E L T
Sbjct:   72 ERVNRESKKGKQKGRTLEIERLIGRSLRAICDMKLLGEREVTVDCDVLQADGGTRTASICGGFVALHDACTSLMERGLIKVHPLTDFCAAISVGIVDGTPKLDLPYVEDSTASVDMNVVMTGSGRFVEVQGTAEGDPFDRSELDALTSLAEKGISEIIAMQKEILGT 238
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BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Match: A0A0G3V456_9ACTN (Ribonuclease PH n=6 Tax=root TaxID=1 RepID=A0A0G3V456_9ACTN)

HSP 1 Score: 218 bits (556), Expect = 5.730e-69
Identity = 117/174 (67.24%), Postives = 135/174 (77.59%), Query Frame = 1 
Query:    1 PERVNRERRGAK--GRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQADGGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPVLDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAEGGIATITAAQRETLATAPSPRS 516
            PERV+RE    K  GRTQEI+RLIGRSLRAVTD+ ALGE  I +DCD LQADGGTRTA+ICG ++ALHDACER+VQ G +  NP+   CAA+SVGIV   P LDL Y EDS+A+VD NV+MTG G+ IEVQGTAEG  F R ELD+LL LAEGGIA + A QRE L+  PSPRS
Sbjct:   73 PERVSREAAKGKQSGRTQEIQRLIGRSLRAVTDLVALGEFQITVDCDALQADGGTRTAAICGGYLALHDACERMVQQGRLRINPVGELCAAVSVGIVGALPSLDLDYVEDSSAEVDMNVVMTGDGKFIEVQGTAEGAAFSRGELDSLLGLAEGGIAELFAMQREMLSVPPSPRS 246
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BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Match: A0A7W0QXI1_9ACTN (Ribonuclease PH n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A7W0QXI1_9ACTN)

HSP 1 Score: 218 bits (556), Expect = 5.730e-69
Identity = 118/176 (67.05%), Postives = 131/176 (74.43%), Query Frame = 1 
Query:    4 ERVNRERRGAK--GRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQADGGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPVLDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAEGGIATITAAQRETLATAPSPRSPGR 525
            ER+ RE    K  GRTQEI+RLIGRSLR+V DM  LGE  + +DCDVLQADGGTRTA+ICG +VALHDA  RLVQ G I  NPL   C AISVGIV G+ +LDLPY EDS A VD NV+MTG GQ IEVQGTAEG +F R ELD LL LAE GIA I   Q E LAT P+PR+PGR
Sbjct:   72 ERIGREAAKGKQSGRTQEIQRLIGRSLRSVCDMSVLGERQVIVDCDVLQADGGTRTAAICGGYVALHDAFTRLVQNGRITANPLTDSCGAISVGIVGGTALLDLPYEEDSRAGVDMNVVMTGAGQFIEVQGTAEGKSFSREELDRLLGLAEVGIAEIGRLQTELLATPPTPRAPGR 247
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig114353.2997.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3C0HSC0_9ACTN4.310e-10087.43Ribonuclease PH n=6 Tax=Acidimicrobiaceae bacteriu... [more]
A0A5Q2RLF6_9ACTN4.650e-7671.68Ribonuclease PH n=1 Tax=Actinomarinicola tropica T... [more]
A0A7X6ART5_9ACTN5.620e-7674.55Ribonuclease PH (Fragment) n=1 Tax=Actinomycetia b... [more]
A0A6B1AVU3_9ACTN2.790e-7472.09Ribonuclease PH n=1 Tax=Acidimicrobiaceae bacteriu... [more]
A0A3S0DTR6_9ACTN7.050e-7267.43Ribonuclease PH n=2 Tax=Acidimicrobiia TaxID=84992... [more]
A0A7Y2GKH5_9ACTN1.170e-7169.01Ribonuclease PH n=1 Tax=Acidimicrobiales bacterium... [more]
A0A2E7NHQ5_9BACT3.140e-6967.27Ribonuclease PH n=6 Tax=root TaxID=1 RepID=A0A2E7N... [more]
A0A2E9XE16_9ACTN4.590e-6968.86Ribonuclease PH n=3 Tax=Actinobacteria TaxID=20117... [more]
A0A0G3V456_9ACTN5.730e-6967.24Ribonuclease PH n=6 Tax=root TaxID=1 RepID=A0A0G3V... [more]
A0A7W0QXI1_9ACTN5.730e-6967.05Ribonuclease PH n=1 Tax=Acidimicrobiia bacterium T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig114353contigM-pyrifera_M_contig114353:3..530 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score218.8
Seed ortholog evalue2e-54
Seed eggNOG ortholog1229780.BN381_80058
Preferred namerph
KEGG rclassRC00002
KEGG koko:K00989,ko:K02428
KEGG ReactionR00426,R00720,R01855,R02100,R02720,R03531
KEGG Pathwayko00230,map00230
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004518,GO:0004540,GO:0006139,GO:0006364,GO:0006396,GO:0006399,GO:0006401,GO:0006725,GO:0006807,GO:0008033,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009987,GO:0010467,GO:0016070,GO:0016072,GO:0016075,GO:0016787,GO:0016788,GO:0019439,GO:0022613,GO:0031123,GO:0031125,GO:0034470,GO:0034641,GO:0034655,GO:0034660,GO:0034661,GO:0042254,GO:0043170,GO:0043628,GO:0044085,GO:0044237,GO:0044238,GO:0044248,GO:0044260,GO:0044265,GO:0044270,GO:0046483,GO:0046700,GO:0071704,GO:0071840,GO:0090304,GO:0090305,GO:0090501,GO:0140098,GO:1901360,GO:1901361,GO:1901575
EggNOG free text desc.Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates
EggNOG OGs2GJFI@201174,3UWBC@52018,COG0689@1,COG0689@2
EC2.7.7.56,3.6.1.66
COG Functional cat.J
Best tax levelunclassified Actinobacteria (class)
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03016
Exons1
Model size528
Cds size528
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815660.1438968-CDS-M-pyrifera_M_contig114353:2..5301622815660.1438968-CDS-M-pyrifera_M_contig114353:2..530Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig114353 3..530 +
1692277509.4491677-CDS-M-pyrifera_M_contig114353:2..5301692277509.4491677-CDS-M-pyrifera_M_contig114353:2..530Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig114353 3..530 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig114353.2997.1prot_M-pyrifera_M_contig114353.2997.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig114353 3..530 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig114353.2997.1

>prot_M-pyrifera_M_contig114353.2997.1 ID=prot_M-pyrifera_M_contig114353.2997.1|Name=mRNA_M-pyrifera_M_contig114353.2997.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=176bp
PERVNRERRGAKGRTQEIERLIGRSLRAVTDMRALGEHSIRLDCDVLQAD
GGTRTASICGAFVALHDACERLVQGGAIAKNPLVTECAAISVGIVDGSPV
LDLPYPEDSTADVDFNVIMTGTGQLIEVQGTAEGNTFDRAELDALLDLAE
GGIATITAAQRETLATAPSPRSPGR*
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mRNA from alignment at M-pyrifera_M_contig114353:3..530+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig114353.2997.1 ID=mRNA_M-pyrifera_M_contig114353.2997.1|Name=mRNA_M-pyrifera_M_contig114353.2997.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=528bp|location=Sequence derived from alignment at M-pyrifera_M_contig114353:3..530+ (Macrocystis pyrifera P11B4 male)
CCCGAGCGGGTCAACCGTGAGCGTCGCGGCGCCAAGGGTCGCACCCAGGA
GATTGAGCGGCTCATCGGCCGTTCGCTGCGTGCCGTCACCGACATGAGGG
CACTCGGCGAGCACTCGATCCGCCTCGACTGCGACGTGCTCCAGGCCGAC
GGCGGGACCCGCACGGCGTCGATCTGTGGCGCGTTCGTGGCGTTGCACGA
TGCATGCGAACGGCTCGTGCAGGGTGGCGCGATCGCGAAGAACCCCCTGG
TCACCGAGTGTGCAGCGATCTCCGTGGGCATCGTCGACGGCAGCCCGGTG
CTCGATCTTCCGTACCCCGAGGACTCCACGGCCGACGTCGACTTCAACGT
GATCATGACCGGCACCGGCCAGCTCATCGAGGTGCAGGGCACCGCCGAGG
GCAACACTTTCGATCGGGCCGAGCTCGATGCCCTGCTCGACCTCGCCGAG
GGCGGCATCGCCACCATCACTGCCGCGCAACGCGAGACCCTGGCCACCGC
GCCCAGCCCCCGCTCCCCGGGGCGCTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig114353:3..530+

>mRNA_M-pyrifera_M_contig114353.2997.1 ID=mRNA_M-pyrifera_M_contig114353.2997.1|Name=mRNA_M-pyrifera_M_contig114353.2997.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1056bp|location=Sequence derived from alignment at M-pyrifera_M_contig114353:3..530+ (Macrocystis pyrifera P11B4 male)
CCCGAGCGGGTCAACCGTGAGCGTCGCGGCGCCAAGGGTCGCACCCAGGA
GATTGAGCGGCTCATCGGCCGTTCGCTGCGTGCCGTCACCGACATGAGGG
CACTCGGCGAGCACTCGATCCGCCTCGACTGCGACGTGCTCCAGGCCGAC
GGCGGGACCCGCACGGCGTCGATCTGTGGCGCGTTCGTGGCGTTGCACGA
TGCATGCGAACGGCTCGTGCAGGGTGGCGCGATCGCGAAGAACCCCCTGG
TCACCGAGTGTGCAGCGATCTCCGTGGGCATCGTCGACGGCAGCCCGGTG
CTCGATCTTCCGTACCCCGAGGACTCCACGGCCGACGTCGACTTCAACGT
GATCATGACCGGCACCGGCCAGCTCATCGAGGTGCAGGGCACCGCCGAGG
GCAACACTTTCGATCGGGCCGAGCTCGATGCCCTGCTCGACCTCGCCGAG
GGCGGCATCGCCACCATCACTGCCGCGCAACGCGAGACCCTGGCCACCGC
GCCCAGCCCCCGCTCCCCGGGGCGCTGACCCGAGCGGGTCAACCGTGAGC
GTCGCGGCGCCAAGGGTCGCACCCAGGAGATTGAGCGGCTCATCGGCCGT
TCGCTGCGTGCCGTCACCGACATGAGGGCACTCGGCGAGCACTCGATCCG
CCTCGACTGCGACGTGCTCCAGGCCGACGGCGGGACCCGCACGGCGTCGA
TCTGTGGCGCGTTCGTGGCGTTGCACGATGCATGCGAACGGCTCGTGCAG
GGTGGCGCGATCGCGAAGAACCCCCTGGTCACCGAGTGTGCAGCGATCTC
CGTGGGCATCGTCGACGGCAGCCCGGTGCTCGATCTTCCGTACCCCGAGG
ACTCCACGGCCGACGTCGACTTCAACGTGATCATGACCGGCACCGGCCAG
CTCATCGAGGTGCAGGGCACCGCCGAGGGCAACACTTTCGATCGGGCCGA
GCTCGATGCCCTGCTCGACCTCGCCGAGGGCGGCATCGCCACCATCACTG
CCGCGCAACGCGAGACCCTGGCCACCGCGCCCAGCCCCCGCTCCCCGGGG
CGCTGA
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