mRNA_M-pyrifera_M_contig11401.2911.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig11401.2911.1
Unique NamemRNA_M-pyrifera_M_contig11401.2911.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig11401.2911.1 vs. uniprot
Match: D8LJ65_ECTSI (tRNA-i(6)A37 thiotransferase enzyme MiaB n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LJ65_ECTSI)

HSP 1 Score: 145 bits (365), Expect = 6.510e-39
Identity = 81/131 (61.83%), Postives = 94/131 (71.76%), Query Frame = 1 
Query:    1 SCSGFAWPLSPSVANVGQGIRSG-DGRQSTVAAMTTPRGRLLAATTDGGGATTAVATEGVRRKVVKPWLTKAQETVDYSNVEAMYVRHILVQTEAIAKMCLNQLRDGADFAELASSISDCQATRTKGGEVG 390
            SC+   + L P+    G+      D RQ+T AA     GR L A +   G+TT VAT+  R+KVVKPW TKAQETVDYSNVEAMYVRHILVQTE +AK CL Q+R+GADF ELASSISDCQATR KGGE+G
Sbjct:   22 SCAATGFTLPPTTPRTGRATAGARDVRQATPAAEPPRAGRPLFADSSSDGSTT-VATQPKRKKVVKPWFTKAQETVDYSNVEAMYVRHILVQTEGLAKTCLEQIREGADFGELASSISDCQATRAKGGEIG 151
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BLAST of mRNA_M-pyrifera_M_contig11401.2911.1 vs. uniprot
Match: A0A6H5KNX7_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5KNX7_9PHAE)

HSP 1 Score: 142 bits (357), Expect = 9.940e-37
Identity = 82/131 (62.60%), Postives = 95/131 (72.52%), Query Frame = 1 
Query:    1 SCSGFAWPLSPSVANVGQGIRSG-DGRQSTVAAMTTPRGRLLAATTDGGGATTAVATEGVRRKVVKPWLTKAQETVDYSNVEAMYVRHILVQTEAIAKMCLNQLRDGADFAELASSISDCQATRTKGGEVG 390
            + +GF  P  P+ A  G+      D RQ+T AA  +  GR L A +   G+T AVAT+  R+KVVKPW TKAQETVDYSNVEAMYVRHILVQTE +AK CL Q+R+G DF ELASSISDCQATR KGGEVG
Sbjct:   25 AATGFTLP--PTTARTGRATAGPRDVRQATPAAEPSRGGRPLFADSSSDGST-AVATQPKRKKVVKPWFTKAQETVDYSNVEAMYVRHILVQTEGLAKTCLEQIREGVDFGELASSISDCQATRAKGGEVG 152
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BLAST of mRNA_M-pyrifera_M_contig11401.2911.1 vs. uniprot
Match: A0A7S1KF02_9ALVE (Hypothetical protein (Fragment) n=1 Tax=Vitrella brassicaformis TaxID=1169539 RepID=A0A7S1KF02_9ALVE)

HSP 1 Score: 63.9 bits (154), Expect = 2.460e-9
Identity = 36/71 (50.70%), Postives = 50/71 (70.42%), Query Frame = 1 
Query:  187 VVKP-WL-TKAQETVDYSNVEAMYVRHILVQTEAIAKMCLNQLRDG-ADFAELASSISDCQATRTKGGEVG 390
            +VKP W  T+AQE VD   V+A+ V HIL++TE++A++ L +LR G  DFA+LA   S CQAT+   G+VG
Sbjct:   88 LVKPGWFPTRAQEVVDTRTVQAVRVSHILLETESLAELALERLRQGDVDFADLAEVASICQATKQDKGQVG 158
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BLAST of mRNA_M-pyrifera_M_contig11401.2911.1 vs. uniprot
Match: A0A0G4ERE5_VITBC (Uncharacterized protein n=1 Tax=Vitrella brassicaformis (strain CCMP3155) TaxID=1169540 RepID=A0A0G4ERE5_VITBC)

HSP 1 Score: 63.9 bits (154), Expect = 2.750e-9
Identity = 36/71 (50.70%), Postives = 50/71 (70.42%), Query Frame = 1 
Query:  187 VVKP-WL-TKAQETVDYSNVEAMYVRHILVQTEAIAKMCLNQLRDG-ADFAELASSISDCQATRTKGGEVG 390
            +VKP W  T+AQE VD   V+A+ V HIL++TE++A++ L +LR G  DFA+LA   S CQAT+   G+VG
Sbjct:   28 LVKPGWFPTRAQEVVDTRTVQAVRVSHILLETESLAELALERLRQGDVDFADLAEVASICQATKQDKGQVG 98
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BLAST of mRNA_M-pyrifera_M_contig11401.2911.1 vs. uniprot
Match: A0A7S1HKL2_HEMAN (Peptidyl-prolyl cis-trans isomerase (Fragment) n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1HKL2_HEMAN)

HSP 1 Score: 60.8 bits (146), Expect = 5.130e-9
Identity = 32/66 (48.48%), Postives = 42/66 (63.64%), Query Frame = 1 
Query:  193 KPWLTKAQETVDYSNVEAMYVRHILVQTEAIAKMCLNQLRDGADFAELASSISDCQATRTKGGEVG 390
            K W  + ++      V ++ V HILV T  +A   + QLR+GADFA LAS+IS C+ TR KGGEVG
Sbjct:   94 KKWFEETEKQRQ-GKVVSVRVSHILVSTSEMASTLMTQLREGADFAGLASAISMCEGTREKGGEVG 158
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BLAST of mRNA_M-pyrifera_M_contig11401.2911.1 vs. uniprot
Match: A0A7S2S7D0_9STRA (Hypothetical protein n=1 Tax=Rhizochromulina marina TaxID=1034831 RepID=A0A7S2S7D0_9STRA)

HSP 1 Score: 57.4 bits (137), Expect = 5.280e-7
Identity = 26/67 (38.81%), Postives = 44/67 (65.67%), Query Frame = 1 
Query:  193 KPWL-TKAQETVDYSNVEAMYVRHILVQTEAIAKMCLNQLRDGADFAELASSISDCQATRTKGGEVG 390
            K W  TK  + ++  +V A+ V H+L+ T+ +A  CL Q++ G DF +LA +IS C+ +R +GG++G
Sbjct:   47 KEWYPTKISQVIESKDVMAVRVSHVLLHTKEMATACLEQVKSGRDFNDLALAISICEESREEGGKIG 113
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BLAST of mRNA_M-pyrifera_M_contig11401.2911.1 vs. uniprot
Match: A0A101VV54_9FIRM (Peptidylprolyl isomerase n=1 Tax=Gracilibacter sp. BRH_c7a TaxID=1734398 RepID=A0A101VV54_9FIRM)

HSP 1 Score: 54.7 bits (130), Expect = 3.610e-6
Identity = 29/78 (37.18%), Postives = 47/78 (60.26%), Query Frame = 1 
Query:  184 KVVKPWLTKAQETVD---------YSNVEAMYVRHILVQTEAIAKMCLNQLRDGADFAELASSISDCQATRTKGGEVG 390
            K++ P +T AQE ++         ++  EA+  RHILV+TE +AK   N+L DG DF++LA   S   + + +GG++G
Sbjct:  127 KLIGPGITIAQEEIEEYFEMYKDSFAVAEAVKARHILVETEEVAKEVKNKLADGEDFSKLAKQYSIDDSNKEQGGDLG 204
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BLAST of mRNA_M-pyrifera_M_contig11401.2911.1 vs. uniprot
Match: D1C3R6_SPHTD (PpiC-type peptidyl-prolyl cis-trans isomerase n=1 Tax=Sphaerobacter thermophilus (strain DSM 20745 / S 6022) TaxID=479434 RepID=D1C3R6_SPHTD)

HSP 1 Score: 50.8 bits (120), Expect = 9.700e-5
Identity = 35/81 (43.21%), Postives = 42/81 (51.85%), Query Frame = 1 
Query:  169 EGVRRKVVKPWLTKAQETVDYS-------NVEAMYVRHILVQTEAIAKMCLNQLRDGADFAELASSISDCQATRTKGGEVG 390
            E  RR VV P    A++ V Y+         E ++  HILV TE  AK    QLR GADFAELA   S   AT   GG++G
Sbjct:  267 EDFRRLVVLP--EAARQKVVYALQEKIPTRAEQVHAAHILVATEDAAKAIEEQLRQGADFAELAKEQSADSATAVNGGDLG 345
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig11401.2911.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 8
Match NameE-valueIdentityDescription
D8LJ65_ECTSI6.510e-3961.83tRNA-i(6)A37 thiotransferase enzyme MiaB n=1 Tax=E... [more]
A0A6H5KNX7_9PHAE9.940e-3762.60Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A7S1KF02_9ALVE2.460e-950.70Hypothetical protein (Fragment) n=1 Tax=Vitrella b... [more]
A0A0G4ERE5_VITBC2.750e-950.70Uncharacterized protein n=1 Tax=Vitrella brassicaf... [more]
A0A7S1HKL2_HEMAN5.130e-948.48Peptidyl-prolyl cis-trans isomerase (Fragment) n=1... [more]
A0A7S2S7D0_9STRA5.280e-738.81Hypothetical protein n=1 Tax=Rhizochromulina marin... [more]
A0A101VV54_9FIRM3.610e-637.18Peptidylprolyl isomerase n=1 Tax=Gracilibacter sp.... [more]
D1C3R6_SPHTD9.700e-543.21PpiC-type peptidyl-prolyl cis-trans isomerase n=1 ... [more]
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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig11401contigM-pyrifera_M_contig11401:178..4126 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score146.0
Seed ortholog evalue1.2e-32
Seed eggNOG ortholog2880.D8LJ65
Hectar predicted targeting categoryother localisation
EggNOG free text desc.N6-isopentenyladenosine methylthiotransferase activity
EggNOG OGsCOG0621@1,KOG2492@2759
COG Functional cat.J
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons2
Model size390
Cds size390
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815654.9074163-CDS-M-pyrifera_M_contig11401:177..3191622815654.9074163-CDS-M-pyrifera_M_contig11401:177..319Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig11401 178..319 -
1692277507.0210202-CDS-M-pyrifera_M_contig11401:177..3191692277507.0210202-CDS-M-pyrifera_M_contig11401:177..319Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig11401 178..319 -
1622815654.9176857-CDS-M-pyrifera_M_contig11401:3878..41261622815654.9176857-CDS-M-pyrifera_M_contig11401:3878..4126Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig11401 3879..4126 -
1692277507.032029-CDS-M-pyrifera_M_contig11401:3878..41261692277507.032029-CDS-M-pyrifera_M_contig11401:3878..4126Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig11401 3879..4126 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig11401.2911.1prot_M-pyrifera_M_contig11401.2911.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig11401 178..4126 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig11401.2911.1

>prot_M-pyrifera_M_contig11401.2911.1 ID=prot_M-pyrifera_M_contig11401.2911.1|Name=mRNA_M-pyrifera_M_contig11401.2911.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=130bp
SCSGFAWPLSPSVANVGQGIRSGDGRQSTVAAMTTPRGRLLAATTDGGGA
TTAVATEGVRRKVVKPWLTKAQETVDYSNVEAMYVRHILVQTEAIAKMCL
NQLRDGADFAELASSISDCQATRTKGGEVG
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mRNA from alignment at M-pyrifera_M_contig11401:178..4126-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig11401.2911.1 ID=mRNA_M-pyrifera_M_contig11401.2911.1|Name=mRNA_M-pyrifera_M_contig11401.2911.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=3949bp|location=Sequence derived from alignment at M-pyrifera_M_contig11401:178..4126- (Macrocystis pyrifera P11B4 male)
TCCTGCAGCGGGTTCGCTTGGCCACTGTCACCATCCGTAGCCAATGTCGG
GCAAGGAATAAGGTCTGGTGACGGGCGGCAGTCGACGGTGGCCGCGATGA
CGACGCCCCGCGGACGTCTGTTGGCAGCAACGACGGACGGAGGGGGTGCT
ACTACTGCTGTAGCGACCGAAGGCGTCAGGCGGAAGGTCGTGAAGCCGTG
GTTGACAAAGGCACAGGAGACGGTAGATTACAGCAATGTCGAGGCAATGT
GAGGGGATGAGCTTGTGTGACATACCTGCTGTGGTGTGATACCTATGGCG
TGTACCGCAGTCGCCTATGCATTGTTGTTTCAGAAGAGGCTGCCGTTGTG
CGCGGCTGAGGTCTTGGCTTGAGTTGCACACGCACTATGAGTTTGCAGCC
CGAAATATGCTCGTTACATGCTTCAAGTACCAGGAATGCAAGTATATGGT
ATGCAGTGAGCAAGTAACGGCAGCAGCGCAACATGATCGTACAGAGTGGA
GTCACCCCAGGAGTCATGACTTCTACAGGTATCTCTGGGCCGTGGGACAA
AGAATCGGCACAGACATTGGGTGAGCTGTCGCCTGGTAGAAAGAACCGTG
TTACGTGAAAAACAAACACACCAAATACGCGGTACGAATTCCTCTACTGT
TGTAAGGGGGTACTAAGTACCACTCTGTTCGGAAAAATAAGAATATTTTG
GACTGAGTATTAATATGCAAACGGTCCTTATATTGCTGTACAGAGTGGAA
CCTATCGGGTACCAGACCTGGTATGTGCCAGTGTACCAGTGTACCAGTAC
CAATGACCTCTCGTCTATGGAGCACTGACCCGTCCGAACGCTCAACGAGA
GTTCGCACGGATCATGCCAGGTGTCACGCTGGTACGATCTAAAACGCACT
TGTTTCTTGCAGAGCTCGTACGTAACTTGGCCGAGCCGCGTCCCACCATA
ATGCCACGTAGCCGTCGTATGGGTAGCTTCAAACAACACAGGTACCAGTA
CGGCCAGCTCCGCAAGCGAATGCCGAATGTCGCGGGTAGTCAAGTTACCG
CCAACACTACTAGTAGAACCGTGATATCGTAATCACCGCCACCAACACAT
CCTTGTATACATAACACTGCGTCCGCACCTTATCAGCACTCGAATGACCA
CTGCTAAACCTTCGTGGGATTTTCAGAGGCGAAAATTAACGGTTAAAAAA
ATGACGATGACAGCGAGAGAGGCGGAGAAATTGCACGTATGTGCAAGGCT
CTTCATATTTGCACGTAAATTCACTACGGTTGCATGTACAGCAGGAGTTG
TCGTAGATATGTATGGCCTACATGGTACCAAACGGGATCACGGTGTGAGG
TGGGCCTCCGGCGACTCTTGCAGGGGGCTGATTATATTTCTGCACCACTA
AACGGGAGCAGGGTGCCAAATGGGATGGGCCCACTGCGGGCGGGGTCACA
AGTGCCACATGGGAACAGGGTGCCCAGGTAGTGTAAAAAATGCGTGCAAA
TCTCTAAGAACGTTGCGCGCACGTGCATTTGCTCCGCTGGTGTGACGGCG
ACGGTCCAACATGGTTGGGGATGATACAAAATTGGTGCCCGAGTTGTAAT
GCAAGGAGTCGCCGTAGGCGCGGGCGGGGCGCAACATGATCCCACGTACC
CGTGCTGCTGGACACTCACGATAACAACCATTGTTTCTCGGGGTCCCCCG
GAGAAGTTATTTCGAGGTTTCAGCTACCAGGTACCAGTTAAACTTCAGGT
ATGTGACTACGTGGCGAATCTCCTTACCGCATCACACCGGTGATGAGATA
GCACACCCCCTGGAACGACTTTCGGAGCCGATTTTCTCCAAAACATGTCT
TAAGGGGGGGCGCGCCCAACGACAACGGCGTATTTGGGAAGATGTCGTCG
ACACATTTCCAAAGACGTGTCGTTCGGTGTGTTGCGCCCTTCTTGTTGTC
GAGAAAGTCAGCTTTGCAATTCGTACGATGTGGCTGTGTTATCTTGCGTG
TCACCTGTGACACGGTATCGACGATGTATTGTTTCTCGTCTGTTTCGTAC
CAGGTACTTTGTGACTACAGAACTGTTTTCTCTCTCTTGGGCGAAGAATC
GGCAGGGACAGTGGGAGTGCTGGATATCGACGAGTTTATAGCAATATCGG
TTTAAAATTTCAAGATGGCGCAACACCACAAGTAGACACAACACTCCCTC
GGTCGGACTACGATTTGCCGTGCACCAGCTTGTTACGCTTGGTACCAAGC
AGATACTTGGTTCGAAGGGAGTCAACGGGCGGGTAGAGTATGTGCTGTGG
GCCAAACGAAGAACGACAGCAACAGCGCGAGGCGCCGCCACCAACGTGAA
GTATTCACTGAGTGTCGGCACCCCGCAAGGGGGGTTCGGTGCCCACGGCT
GCTGTATCCCGTGATTGATTCGCCTGCGTACCGCTGCTGTTGCTGTTGTG
GGCTATACCGGTGTCTGCATAGAGCATGACGGGGCGTGATAGCATGCACA
TTTGCTGCGACAACGCACACGTGCTACGTACGTACTCTTGCTGCAGAGCC
GAGGCGCAGCACTCGCATGGCTGTGGCAGAGAGTAGGGAGTTGTCACCGC
TGTGGATTAAAATTCATGATAGCGTACACACTTGCTGCAACAATACACGC
GTTCTACTCGTGCTGAACGTAACTGAGGCACACCGCTCTCGTGGTTGTGG
TAGAGAGTAGGAGGCATTTCTGCGGTGTATTCAAATTCACGCAACACAAG
TTTTTGGAAGGGTGTTTTGGTTGGCGAGGGGGGCTGCTCGACTCTCGTGC
ACAAAATGTCGCAGCCGCACTGCAGCAGTACCTACCCATCGTACAGCGCC
CGACTGCGATCTTGACCGCCGTGTAATAAAAATCTTCTCAACGGTTCGCA
ACGTCTACACTTCAGACAGATCACCGTCTCCCGTCTCATGATCGAGGCCT
CTTGGGGTAAATATATCCTGGATTCCTGATCTGCATGATCTATACGATCT
GTACGATCTAGTTCATGTTGCCGGGTAGGAGCCGTACATACGATCTGCAT
GGTCTAGCACACGTTTCCTGCGGTGCATCTGTATTCTAGACCCTGCGCAA
TATCTCACAACCACAGCTGCGCAGGTTGTAAAAAAAAACGAGTTTCGGCG
AAATCCTCGATCTCACGGCTGCTGTTTTTTTTTTTTTAATATATATATAT
ATATATTGTGTATGGTCTAGATGCTCTTGATCGTAATGTGTCCGATGTGT
CCAACAACTCATCCAGCGACGACGGTGGTAGTGCGGCCTTTAGTCGAGAG
CCACGTGAAACCCCTTTTCCCCTTGTCCGCGACTACTTCGTCTCCCTCTT
CGCGGCCGCCGCCGCCGCCGCCACCGCCACCGCCGTAACCGACAACTTTT
ACTTTGTTCCACATCGTCGCTGACCACAACCTTCCACGCGTCGGATAGAT
CATATAGATCAATCCCGCTTCATGATCTATCTACAGCTCATTCAGGGCAG
ATGTATTCCTGATCTTTATGATCTGTATGATCTGTATGATCTAGCTCGTG
ATGCCGCGTGGGAGCCATGTAATCTGCATGATCTAGCACACGTTTCCTGT
GCTGGATCGATCTGTACTGTACAGAGCCCGCACAGCATTTCATAACGTCA
GGTTAGGGTCTATCTGTAGATATCTATCTGTAAATGATCTATCCTATCTG
TCCGACGTTTGTGACCGTCGAACCCTTCCTGCGATCTGTGGCGTGTGTGT
GATGTTCATATGTACCAATGGCGTTTTTCTGTTGTTCTATCTGACATTGA
CACACAGGTACGTCCGGCACATCCTGGTCCAAACTGAAGCCATAGCCAAG
ATGTGCCTGAACCAACTTAGAGATGGAGCTGACTTTGCGGAGCTGGCGTC
TTCTATCAGCGACTGCCAGGCTACGCGTACGAAGGGTGGAGAGGTAGGG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig11401:178..4126-

>mRNA_M-pyrifera_M_contig11401.2911.1 ID=mRNA_M-pyrifera_M_contig11401.2911.1|Name=mRNA_M-pyrifera_M_contig11401.2911.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=780bp|location=Sequence derived from alignment at M-pyrifera_M_contig11401:178..4126- (Macrocystis pyrifera P11B4 male)
TCCTGCAGCGGGTTCGCTTGGCCACTGTCACCATCCGTAGCCAATGTCGG
GCAAGGAATAAGGTCTGGTGACGGGCGGCAGTCGACGGTGGCCGCGATGA
CGACGCCCCGCGGACGTCTGTTGGCAGCAACGACGGACGGAGGGGGTGCT
ACTACTGCTGTAGCGACCGAAGGCGTCAGGCGGAAGGTCGTGAAGCCGTG
GTTGACAAAGGCACAGGAGACGGTAGATTACAGCAATGTCGAGGCAATTC
CTGCAGCGGGTTCGCTTGGCCACTGTCACCATCCGTAGCCAATGTCGGGC
AAGGAATAAGGTCTGGTGACGGGCGGCAGTCGACGGTGGCCGCGATGACG
ACGCCCCGCGGACGTCTGTTGGCAGCAACGACGGACGGAGGGGGTGCTAC
TACTGCTGTAGCGACCGAAGGCGTCAGGCGGAAGGTCGTGAAGCCGTGGT
TGACAAAGGCACAGGAGACGGTAGATTACAGCAATGTCGAGGCAATGTAC
GTCCGGCACATCCTGGTCCAAACTGAAGCCATAGCCAAGATGTGCCTGAA
CCAACTTAGAGATGGAGCTGACTTTGCGGAGCTGGCGTCTTCTATCAGCG
ACTGCCAGGCTACGCGTACGAAGGGTGGAGAGGTAGGGGTACGTCCGGCA
CATCCTGGTCCAAACTGAAGCCATAGCCAAGATGTGCCTGAACCAACTTA
GAGATGGAGCTGACTTTGCGGAGCTGGCGTCTTCTATCAGCGACTGCCAG
GCTACGCGTACGAAGGGTGGAGAGGTAGGG
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