mRNA_M-pyrifera_M_contig113275.2775.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig113275.2775.1
Unique NamemRNA_M-pyrifera_M_contig113275.2775.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Match: A0A5A8D3A1_CAFRO (Uncharacterized protein n=4 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8D3A1_CAFRO)

HSP 1 Score: 120 bits (300), Expect = 2.580e-29
Identity = 59/109 (54.13%), Postives = 77/109 (70.64%), Query Frame = 1 
Query:    4 AGEEIDEVF-LRFGLSTMAAAALGNLVSDMVGVSSEGVIERRVALPTPRVTEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPLFLSMPEWAHEQMDELKRFLGVSPLSD 327
            AG EID  F ++ GLSTMAAA LGNLVSD++G   EG IER  A+  PR+T  QR   S+K + N GK++GIA+GC+LGMVPL + +P WA ++++  KR LGV P  D
Sbjct:  135 AGTEIDAFFGVKMGLSTMAAAGLGNLVSDVMGTGFEGFIERNFAIKQPRLTRAQRRSDSYKYSQNFGKILGIAIGCLLGMVPLLIVLPRWAKQRLESGKRALGVEPPGD 243
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BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Match: A0A1S3K464_LINUN (transmembrane protein 65 isoform X2 n=1 Tax=Lingula unguis TaxID=7574 RepID=A0A1S3K464_LINUN)

HSP 1 Score: 85.9 bits (211), Expect = 3.490e-18
Identity = 47/100 (47.00%), Postives = 63/100 (63.00%), Query Frame = 1 
Query:    4 AGEEIDEVF-LRFGLSTMAAAALGNLVSDMVGVSSEGVIERRVA---LPTPRVTEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPLFLSMPEWAHEQMDE 291
            AGE ID  F    G+STMAAAALGN+VSD+ GV + G++E  V+   +  P +T  Q   RS ++A+ +G+ +GIA GC+LGM PLFL   E      DE
Sbjct:  138 AGEYIDATFGAMLGISTMAAAALGNMVSDVAGVGTAGIVENAVSKLGVKPPNITPSQADHRSTRIASALGRAIGIAFGCLLGMFPLFLLHQEGIFISKDE 237
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BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Match: UPI001ABE997C (transmembrane protein 65 isoform X1 n=4 Tax=Bufo TaxID=8383 RepID=UPI001ABE997C)

HSP 1 Score: 85.1 bits (209), Expect = 7.710e-18
Identity = 45/104 (43.27%), Postives = 66/104 (63.46%), Query Frame = 1 
Query:    1 AAGEEID-EVFLRFGLSTMAAAALGNLVSDMVGVSSEGVIER---RVALPTPRVTEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPLFLSMPEWAHEQMDELKR 300
            AAG +I+  +    G+STMAAAALGNLVSD+ G+   G +E    R+ +P P +T +Q  +R  +V+ +MGK +G+A+GC+LGM PL      W  E+  E K+
Sbjct:  154 AAGTQIEFHIGALLGISTMAAAALGNLVSDIAGLGLAGYVEAISSRLGVPIPDLTPKQADMRQTRVSAHMGKAIGVAIGCILGMFPLLFF--NWDEEEKSEKKK 255
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BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Match: A0A5N5NFW6_PANHP (Uncharacterized protein n=1 Tax=Pangasianodon hypophthalmus TaxID=310915 RepID=A0A5N5NFW6_PANHP)

HSP 1 Score: 83.2 bits (204), Expect = 2.550e-17
Identity = 44/113 (38.94%), Postives = 74/113 (65.49%), Query Frame = 1 
Query:    1 AAGEEID-EVFLRFGLSTMAAAALGNLVSDMVGVSSEGVIER---RVALPTPRVTEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPLFLSMPEWAHEQMDELKRFLGVSPLSD 327
            AAG +I+  + + FG+STMAAAALGNLVSD+ G+   G +E    R+ +  P +T +Q  +   ++++++GK +G+ +GC+LGM PLF     +  ++ +E  +  GV+PL+D
Sbjct:  118 AAGTQIELSIGVTFGISTMAAAALGNLVSDLAGLGLAGYVEALAARLGMQIPDLTPKQADMWQTRLSSHLGKAIGVTIGCLLGMFPLF-----FLGDEDEEETQSAGVTPLAD 225
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BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Match: A0A3B4DQ55_PYGNA (Transmembrane protein 65 n=2 Tax=Pygocentrus nattereri TaxID=42514 RepID=A0A3B4DQ55_PYGNA)

HSP 1 Score: 82.0 bits (201), Expect = 7.840e-17
Identity = 43/100 (43.00%), Postives = 65/100 (65.00%), Query Frame = 1 
Query:    1 AAGEEID-EVFLRFGLSTMAAAALGNLVSDMVGVSSEGVIER---RVALPTPRVTEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPLFLSMPEWAHEQMD 288
            AAG +I+  + + FG+STMAAAALGNLVSD+ G+   G +E    R+ +  P +T +Q  +   +++++MGK +G+ +GC+LGM PLF    E   E MD
Sbjct:  124 AAGTQIELSIGVTFGISTMAAAALGNLVSDLAGLGLAGYVEALAARLGMQIPDLTPKQVDMWQTRLSSHMGKAIGVTIGCLLGMFPLFFLSDEDEEETMD 223
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BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Match: A0A2C9L947_BIOGL (Uncharacterized protein n=1 Tax=Biomphalaria glabrata TaxID=6526 RepID=A0A2C9L947_BIOGL)

HSP 1 Score: 79.3 bits (194), Expect = 8.830e-17
Identity = 41/86 (47.67%), Postives = 58/86 (67.44%), Query Frame = 1 
Query:    4 AGEEIDEVF-LRFGLSTMAAAALGNLVSDMVGVSSEGVIE---RRVALPTPRVTEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPL 249
            AGE ID    + FG+STMAAAALGNL+SD+ G+ S   +E    +V +  P ++  Q  + S K A+NMGK++G+ +GC+LGM PL
Sbjct:   18 AGEYIDVTLGVTFGISTMAAAALGNLISDIAGLGSAHYVEILAAKVGMKNPHLSPAQVDMGSTKFASNMGKIIGVTIGCLLGMFPL 103
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BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Match: A0A3B4DRJ7_PYGNA (Transmembrane protein 65 n=3 Tax=Serrasalmidae TaxID=42495 RepID=A0A3B4DRJ7_PYGNA)

HSP 1 Score: 82.0 bits (201), Expect = 1.090e-16
Identity = 43/100 (43.00%), Postives = 65/100 (65.00%), Query Frame = 1 
Query:    1 AAGEEID-EVFLRFGLSTMAAAALGNLVSDMVGVSSEGVIER---RVALPTPRVTEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPLFLSMPEWAHEQMD 288
            AAG +I+  + + FG+STMAAAALGNLVSD+ G+   G +E    R+ +  P +T +Q  +   +++++MGK +G+ +GC+LGM PLF    E   E MD
Sbjct:  144 AAGTQIELSIGVTFGISTMAAAALGNLVSDLAGLGLAGYVEALAARLGMQIPDLTPKQVDMWQTRLSSHMGKAIGVTIGCLLGMFPLFFLSDEDEEETMD 243
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BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Match: A0A835YYR0_9STRA (GAF domain-containing protein n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835YYR0_9STRA)

HSP 1 Score: 83.2 bits (204), Expect = 1.690e-16
Identity = 50/112 (44.64%), Postives = 69/112 (61.61%), Query Frame = 1 
Query:    4 AGEEIDEVF-LRFGLSTMAAAALGNLVSDMVGVSSEGVIE---RRVALPTPRVTEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPLFLSMPEWAHEQMDELKRFLGVSPLSD 327
            AGE ID    +  G+STMAAAALGNLVSD+ GV   GV+E    ++ LP P ++  Q  LRS + A ++G  +G+ +GC+LGM PL L +P  + E ++E KR      L D
Sbjct:  103 AGEYIDLTLGVTLGISTMAAAALGNLVSDLAGVGLGGVVEGWAAKIGLPDPGLSRAQMNLRSVRTAGHIGCAIGVTIGCLLGMFPLLL-LP--SPEALEEKKRIEKTVALVD 211
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BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Match: F6Y7W5_CIOIN (Transmembrane protein 65-like n=1 Tax=Ciona intestinalis TaxID=7719 RepID=F6Y7W5_CIOIN)

HSP 1 Score: 80.5 bits (197), Expect = 2.640e-16
Identity = 40/88 (45.45%), Postives = 59/88 (67.05%), Query Frame = 1 
Query:    4 AGEEID-EVFLRFGLSTMAAAALGNLVSDMVGVSSEGVIER---RVALPTPRVTEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPLFL 255
            AGE ID ++    G++TMAAAALGNL+SD+ G+   G +E    ++ LPTP +T  Q  L + ++ +N GK +GI +GC++GM PL L
Sbjct:  129 AGEYIDVKIGATLGITTMAAAALGNLISDLAGIGLAGYVELLCCKLGLPTPDLTPAQLELSATRIVSNFGKAMGIVIGCIIGMFPLLL 216
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BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Match: A0A6F9DUL2_9ASCI (Transmembrane protein 65-like n=1 Tax=Phallusia mammillata TaxID=59560 RepID=A0A6F9DUL2_9ASCI)

HSP 1 Score: 80.5 bits (197), Expect = 3.810e-16
Identity = 40/86 (46.51%), Postives = 60/86 (69.77%), Query Frame = 1 
Query:    4 AGEEIDE-VFLRFGLSTMAAAALGNLVSDMVGVSSEGVIER---RVALPTPRVTEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPL 249
            AG+ ID+ + +  G+STMAAAALGNLVSD+ G+   G +E    ++ LP P +T +Q  + S +V +++GK VGI +GC++GM PL
Sbjct:  150 AGDYIDQHIGIALGISTMAAAALGNLVSDLFGIGLAGYVEMLCSKLGLPVPDLTPEQMDMTSSRVYSHLGKAVGITIGCIIGMFPL 235
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig113275.2775.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8D3A1_CAFRO2.580e-2954.13Uncharacterized protein n=4 Tax=Cafeteria roenberg... [more]
A0A1S3K464_LINUN3.490e-1847.00transmembrane protein 65 isoform X2 n=1 Tax=Lingul... [more]
UPI001ABE997C7.710e-1843.27transmembrane protein 65 isoform X1 n=4 Tax=Bufo T... [more]
A0A5N5NFW6_PANHP2.550e-1738.94Uncharacterized protein n=1 Tax=Pangasianodon hypo... [more]
A0A3B4DQ55_PYGNA7.840e-1743.00Transmembrane protein 65 n=2 Tax=Pygocentrus natte... [more]
A0A2C9L947_BIOGL8.830e-1747.67Uncharacterized protein n=1 Tax=Biomphalaria glabr... [more]
A0A3B4DRJ7_PYGNA1.090e-1643.00Transmembrane protein 65 n=3 Tax=Serrasalmidae Tax... [more]
A0A835YYR0_9STRA1.690e-1644.64GAF domain-containing protein n=1 Tax=Tribonema mi... [more]
F6Y7W5_CIOIN2.640e-1645.45Transmembrane protein 65-like n=1 Tax=Ciona intest... [more]
A0A6F9DUL2_9ASCI3.810e-1646.51Transmembrane protein 65-like n=1 Tax=Phallusia ma... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig113275contigM-pyrifera_M_contig113275:144..470 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score81.3
Seed ortholog evalue3.2e-13
Seed eggNOG ortholog7719.XP_002124808.1
Preferred nameTMEM65
Hectar predicted targeting categoryother localisation
GOsGO:0003205,GO:0003231,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005740,GO:0005743,GO:0005886,GO:0005911,GO:0007275,GO:0007507,GO:0008016,GO:0008150,GO:0014704,GO:0016020,GO:0019866,GO:0023051,GO:0030054,GO:0031090,GO:0031966,GO:0031967,GO:0031975,GO:0032501,GO:0032502,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044057,GO:0044291,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0048513,GO:0048731,GO:0048856,GO:0050789,GO:0051239,GO:0065007,GO:0071944,GO:0072359,GO:1903522,GO:1903779
EggNOG free text desc.regulation of cardiac conduction
EggNOG OGs38CNT@33154,3BJAI@33208,3CSC7@33213,485KI@7711,KOG4619@1,KOG4619@2759
COG Functional cat.T
Best tax levelChordata
Best eggNOG OGNA|NA|NA
Exons1
Model size327
Cds size327
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815647.8923123-CDS-M-pyrifera_M_contig113275:143..4701622815647.8923123-CDS-M-pyrifera_M_contig113275:143..470Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig113275 144..470 +
1692277503.9448516-CDS-M-pyrifera_M_contig113275:143..4701692277503.9448516-CDS-M-pyrifera_M_contig113275:143..470Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig113275 144..470 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig113275.2775.1prot_M-pyrifera_M_contig113275.2775.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig113275 144..470 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig113275.2775.1

>prot_M-pyrifera_M_contig113275.2775.1 ID=prot_M-pyrifera_M_contig113275.2775.1|Name=mRNA_M-pyrifera_M_contig113275.2775.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=109bp
AAGEEIDEVFLRFGLSTMAAAALGNLVSDMVGVSSEGVIERRVALPTPRV
TEQQRTLRSFKVATNMGKLVGIAVGCVLGMVPLFLSMPEWAHEQMDELKR
FLGVSPLSD
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mRNA from alignment at M-pyrifera_M_contig113275:144..470+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig113275.2775.1 ID=mRNA_M-pyrifera_M_contig113275.2775.1|Name=mRNA_M-pyrifera_M_contig113275.2775.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=327bp|location=Sequence derived from alignment at M-pyrifera_M_contig113275:144..470+ (Macrocystis pyrifera P11B4 male)
GCAGCTGGCGAGGAGATTGACGAGGTTTTTCTTCGGTTCGGTCTTTCAAC
GATGGCAGCTGCCGCGTTGGGGAATTTGGTGTCGGACATGGTAGGTGTGA
GCAGCGAGGGTGTGATCGAGCGCCGTGTGGCATTGCCCACTCCGCGTGTG
ACGGAGCAACAGCGAACGCTGCGATCGTTCAAGGTGGCGACTAACATGGG
CAAGCTGGTGGGGATTGCGGTGGGGTGTGTGTTGGGCATGGTGCCTCTGT
TCTTGTCGATGCCAGAGTGGGCGCATGAGCAAATGGACGAATTGAAACGG
TTCCTGGGAGTGTCACCACTCTCCGAC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig113275:144..470+

>mRNA_M-pyrifera_M_contig113275.2775.1 ID=mRNA_M-pyrifera_M_contig113275.2775.1|Name=mRNA_M-pyrifera_M_contig113275.2775.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=654bp|location=Sequence derived from alignment at M-pyrifera_M_contig113275:144..470+ (Macrocystis pyrifera P11B4 male)
GCAGCTGGCGAGGAGATTGACGAGGTTTTTCTTCGGTTCGGTCTTTCAAC
GATGGCAGCTGCCGCGTTGGGGAATTTGGTGTCGGACATGGTAGGTGTGA
GCAGCGAGGGTGTGATCGAGCGCCGTGTGGCATTGCCCACTCCGCGTGTG
ACGGAGCAACAGCGAACGCTGCGATCGTTCAAGGTGGCGACTAACATGGG
CAAGCTGGTGGGGATTGCGGTGGGGTGTGTGTTGGGCATGGTGCCTCTGT
TCTTGTCGATGCCAGAGTGGGCGCATGAGCAAATGGACGAATTGAAACGG
TTCCTGGGAGTGTCACCACTCTCCGACGCAGCTGGCGAGGAGATTGACGA
GGTTTTTCTTCGGTTCGGTCTTTCAACGATGGCAGCTGCCGCGTTGGGGA
ATTTGGTGTCGGACATGGTAGGTGTGAGCAGCGAGGGTGTGATCGAGCGC
CGTGTGGCATTGCCCACTCCGCGTGTGACGGAGCAACAGCGAACGCTGCG
ATCGTTCAAGGTGGCGACTAACATGGGCAAGCTGGTGGGGATTGCGGTGG
GGTGTGTGTTGGGCATGGTGCCTCTGTTCTTGTCGATGCCAGAGTGGGCG
CATGAGCAAATGGACGAATTGAAACGGTTCCTGGGAGTGTCACCACTCTC
CGAC
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