mRNA_M-pyrifera_M_contig110662.2243.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig110662.2243.1
Unique NamemRNA_M-pyrifera_M_contig110662.2243.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Match: A0A517QDN6_9PLAN (Phosphate propanoyltransferase n=6 Tax=Gimesia TaxID=1649453 RepID=A0A517QDN6_9PLAN)

HSP 1 Score: 311 bits (797), Expect = 3.890e-106
Identity = 147/154 (95.45%), Postives = 154/154 (100.00%), Query Frame = 1 
Query:    1 TVAIVGPRRRMIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGPQGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCKHS 462
            TVAIVGPRRRMIPNVRVLGPCRG+TQVELAYTDSISLGL+LPVRISGDIKGTPGCVLMGP+GVVEL++GVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKL++PESCKCKHS
Sbjct:   83 TVAIVGPRRRMIPNVRVLGPCRGETQVELAYTDSISLGLELPVRISGDIKGTPGCVLMGPKGVVELEYGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLYIPESCKCKHS 236
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BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Match: A0A518IIV1_9PLAN (Phosphate propanoyltransferase n=12 Tax=Planctomycetaceae TaxID=126 RepID=A0A518IIV1_9PLAN)

HSP 1 Score: 301 bits (772), Expect = 2.500e-102
Identity = 139/154 (90.26%), Postives = 153/154 (99.35%), Query Frame = 1 
Query:    1 TVAIVGPRRRMIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGPQGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCKHS 462
            TVA+VGPRRRMIPNVRVLGPCRGDTQVELA+TD+ISLGLD+P+RISGD+KGTPGCVLMGP+GVVEL+FGVIRAARHVHMSPADCAYYGC+NGD+LHLR++S GCTTVLEDVVVRENP+VKLEVHIDTDEGNAVDLDHATSVKL+VPESCKCKHS
Sbjct:   83 TVAVVGPRRRMIPNVRVLGPCRGDTQVELAFTDTISLGLDVPIRISGDLKGTPGCVLMGPKGVVELEFGVIRAARHVHMSPADCAYYGCSNGDNLHLRIESAGCTTVLEDVVVRENPEVKLEVHIDTDEGNAVDLDHATSVKLYVPESCKCKHS 236
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BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Match: A0A3L7UC36_9BACT (Phosphate propanoyltransferase n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3L7UC36_9BACT)

HSP 1 Score: 244 bits (623), Expect = 9.660e-80
Identity = 112/153 (73.20%), Postives = 131/153 (85.62%), Query Frame = 1 
Query:    1 TVAIVGPRRRMIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGPQGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCKH 459
            TVA+VGPRRRM+PNVRVLGPCR D+QVELA+TD ISLG+DLPVR+SG++KGTPGCVLMGP GVV+L  GVIRA RHVHM P D AYYG ANGD +HLR++SPGCTTVLED+ VR    +KLEVH+DTDEGNA +L++AT V+L  PE C CKH
Sbjct:   78 TVAVVGPRRRMLPNVRVLGPCREDSQVELAFTDGISLGIDLPVRVSGNVKGTPGCVLMGPHGVVDLKHGVIRAMRHVHMGPDDLAYYGVANGDHIHLRIESPGCTTVLEDLAVRAATGIKLEVHLDTDEGNACNLENATKVELVSPEPCHCKH 230
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BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Match: A0A7W0RL50_9PLAN (Phosphate propanoyltransferase n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A7W0RL50_9PLAN)

HSP 1 Score: 245 bits (626), Expect = 1.240e-79
Identity = 110/153 (71.90%), Postives = 136/153 (88.89%), Query Frame = 1 
Query:    1 TVAIVGPRRRMIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGPQGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCKH 459
            T+ I GPRRRMIPNVRVLGPCR D+QVELA+TD ISLG+DLPVRISGD++GTPGCVL+GP+GVV+L  GVIRA RHVH+ PADC +YG  NGD +HLRV+S GCTTVLED+VVR   ++KLEVH+DTDEGNA+DL+HA++V+L+VP++C C+H
Sbjct:  118 TLTIFGPRRRMIPNVRVLGPCRPDSQVELAFTDGISLGIDLPVRISGDVQGTPGCVLVGPKGVVDLQQGVIRAMRHVHIGPADCDFYGVKNGDKVHLRVESRGCTTVLEDLVVRAGDNIKLEVHLDTDEGNAIDLEHASNVELYVPKACACEH 270
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BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Match: UPI0019BA6916 (Phosphate propanoyltransferase n=1 Tax=Fuerstiella sp. TaxID=1936112 RepID=UPI0019BA6916)

HSP 1 Score: 245 bits (625), Expect = 1.450e-79
Identity = 114/153 (74.51%), Postives = 132/153 (86.27%), Query Frame = 1 
Query:    1 TVAIVGPRRRMIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGPQGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCKH 459
            TVA+VGPRRRM+PNVRVLGPCR D+QVELA+TD ISLG+DLPVRISG++KGTPGCVLMGP GVVEL  GVIRA RHVHM P D AYYG ANGD +HLR++SPGCTTVLED+ VR    +KLEVH+DTDEGNA +L++AT V+L  PE C+CKH
Sbjct:  112 TVAVVGPRRRMLPNVRVLGPCREDSQVELAFTDGISLGIDLPVRISGNVKGTPGCVLMGPHGVVELKQGVIRAMRHVHMGPDDLAYYGVANGDLVHLRIESPGCTTVLEDLAVRAAKGIKLEVHLDTDEGNACNLENATKVELVKPEPCQCKH 264
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BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Match: A0A518CI08_9PLAN (Phosphate propanoyltransferase n=1 Tax=Polystyrenella longa TaxID=2528007 RepID=A0A518CI08_9PLAN)

HSP 1 Score: 243 bits (621), Expect = 2.530e-79
Identity = 113/153 (73.86%), Postives = 131/153 (85.62%), Query Frame = 1 
Query:    1 TVAIVGPRRRMIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGPQGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCKH 459
            TVA+VGPRRRMIPNVRVLGPCRGD+Q+ELA+TD ISLG+DLPVR+SGDIKGTPGCVL+GP GVVEL  GVIRA RHVHM PAD  YYG  NG+ ++LRV+S GCTTVLED+ VR + D+KLEVH+DTDEGNA++LD AT V+L   E C CKH
Sbjct:   84 TVAVVGPRRRMIPNVRVLGPCRGDSQIELAFTDGISLGIDLPVRVSGDIKGTPGCVLVGPAGVVELKQGVIRAMRHVHMGPADLKYYGVENGEKMNLRVESEGCTTVLEDLQVRADKDIKLEVHLDTDEGNAINLDRATKVELLKKEPCACKH 236
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BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Match: A0A353Z781_9PLAN (Phosphate propanoyltransferase (Fragment) n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A353Z781_9PLAN)

HSP 1 Score: 240 bits (612), Expect = 4.440e-79
Identity = 114/153 (74.51%), Postives = 130/153 (84.97%), Query Frame = 1 
Query:    1 TVAIVGPRRRMIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGPQGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCKH 459
            TVA+VGPRRRM+P VRVLGPCRG TQVELA+TD ISLGLDLPVRISGDIKGTPGCVL+GPQGVVEL+ GVIRA RHVHM P D  YYG  +GD ++LRV+SPGCTTVLED+ VR   ++KLEVH+DTDEGNAV+L+ AT V+L   E C CKH
Sbjct:    7 TVAVVGPRRRMLPEVRVLGPCRGATQVELAFTDGISLGLDLPVRISGDIKGTPGCVLVGPQGVVELEEGVIRAMRHVHMGPGDLEYYGVNDGDFMNLRVESPGCTTVLEDLRVRFGKEIKLEVHLDTDEGNAVNLEQATKVELLKAEPCSCKH 159
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BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Match: A0A1P8WII7_9PLAN (Phosphate propanoyltransferase n=1 Tax=Fuerstiella marisgermanici TaxID=1891926 RepID=A0A1P8WII7_9PLAN)

HSP 1 Score: 242 bits (618), Expect = 1.140e-78
Identity = 112/153 (73.20%), Postives = 131/153 (85.62%), Query Frame = 1 
Query:    1 TVAIVGPRRRMIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGPQGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCKH 459
            TVA+VGPRRRMIPNVRVLGPCR D+QVELA+TD ISLG+DLPVRISGD++GTPGCVL+GP+GVVEL  GVIRA RHVHM PAD  +YG  N D++HLRV+SPGCTTVLED+ VR   ++KLEVH+DTDEGNA++L+ AT V L  PE C CKH
Sbjct:  100 TVAVVGPRRRMIPNVRVLGPCRDDSQVELAFTDGISLGIDLPVRISGDVQGTPGCVLVGPKGVVELQQGVIRAMRHVHMGPADLVHYGVKNKDTIHLRVESPGCTTVLEDLTVRAGDNIKLEVHLDTDEGNAINLEKATKVDLVKPEPCACKH 252
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BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Match: UPI00141E85B2 (phosphate propanoyltransferase n=1 Tax=Rubinisphaera sp. JC658 TaxID=2713176 RepID=UPI00141E85B2)

HSP 1 Score: 239 bits (611), Expect = 6.890e-78
Identity = 108/152 (71.05%), Postives = 132/152 (86.84%), Query Frame = 1 
Query:    1 TVAIVGPRRRMIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGPQGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCK 456
            TVA+VGPRRRMIPNVRVLGPCRGD+QVELA+TD ISLG+DLPVRISGD++GTPGC+L+GP+GVV+LD GVIRA RHVHM P D  YY   NGD LHL+++SPGCTT LE++VVR   ++KLEVH+DTDEGNA +L+HAT+V+L  P +C C+
Sbjct:   79 TVAVVGPRRRMIPNVRVLGPCRGDSQVELAFTDGISLGIDLPVRISGDVQGTPGCILVGPKGVVDLDQGVIRAMRHVHMGPNDLEYYNVKNGDELHLKIESPGCTTTLENLVVRAGENIKLEVHLDTDEGNAANLEHATNVELVKPATCGCQ 230
[Close]
BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Match: A0A517T3E6_9PLAN (Phosphate propanoyltransferase n=1 Tax=Calycomorphotria hydatis TaxID=2528027 RepID=A0A517T3E6_9PLAN)

HSP 1 Score: 239 bits (611), Expect = 9.850e-78
Identity = 110/152 (72.37%), Postives = 132/152 (86.84%), Query Frame = 1 
Query:    1 TVAIVGPRRRMIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGPQGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLEDVVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCK 456
            TV +VGPR+RM+PNVRVLGPCRGDTQVELA+TD ISLG+DLPVRISGDI+GTPGCVL+GP+GVVEL  GVIRA RHVHM PAD A+Y  +NGD +HLR++SPGCTTVLED+ VR + ++KLEVH+DTDEGNAV+L HAT V+L   + C C+
Sbjct:   90 TVMVVGPRKRMLPNVRVLGPCRGDTQVELAFTDGISLGIDLPVRISGDIQGTPGCVLVGPKGVVELKQGVIRAMRHVHMGPADLAHYDVSNGDQMHLRIESPGCTTVLEDLTVRASDNIKLEVHLDTDEGNAVNLQHATHVELLKSKPCACQ 241
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig110662.2243.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A517QDN6_9PLAN3.890e-10695.45Phosphate propanoyltransferase n=6 Tax=Gimesia Tax... [more]
A0A518IIV1_9PLAN2.500e-10290.26Phosphate propanoyltransferase n=12 Tax=Planctomyc... [more]
A0A3L7UC36_9BACT9.660e-8073.20Phosphate propanoyltransferase n=1 Tax=Planctomyce... [more]
A0A7W0RL50_9PLAN1.240e-7971.90Phosphate propanoyltransferase n=1 Tax=Planctomyce... [more]
UPI0019BA69161.450e-7974.51Phosphate propanoyltransferase n=1 Tax=Fuerstiella... [more]
A0A518CI08_9PLAN2.530e-7973.86Phosphate propanoyltransferase n=1 Tax=Polystyrene... [more]
A0A353Z781_9PLAN4.440e-7974.51Phosphate propanoyltransferase (Fragment) n=1 Tax=... [more]
A0A1P8WII7_9PLAN1.140e-7873.20Phosphate propanoyltransferase n=1 Tax=Fuerstiella... [more]
UPI00141E85B26.890e-7871.05phosphate propanoyltransferase n=1 Tax=Rubinisphae... [more]
A0A517T3E6_9PLAN9.850e-7872.37Phosphate propanoyltransferase n=1 Tax=Calycomorph... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig110662contigM-pyrifera_M_contig110662:3..467 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score283.9
Seed ortholog evalue4.3e-74
Seed eggNOG ortholog344747.PM8797T_14791
KEGG rclassRC00004,RC02746,RC02816
KEGG koko:K15024
KEGG ReactionR00230,R00921
KEGG Pathwayko00430,ko00620,ko00640,ko00720,ko01100,ko01120,ko01200,map00430,map00620,map00640,map00720,map01100,map01120,map01200
KEGG ModuleM00579
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Involved in 1,2-propanediol (1,2-PD) degradation by catalyzing the conversion of propanoyl-CoA to propanoyl-phosphate
EggNOG OGs2IY7H@203682,COG4869@1,COG4869@2
EC2.3.1.8
COG Functional cat.Q
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size465
Cds size435
Stop1
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815618.575759-UTR-M-pyrifera_M_contig110662:2..321622815618.575759-UTR-M-pyrifera_M_contig110662:2..32Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig110662 3..32 +
1692277490.145057-UTR-M-pyrifera_M_contig110662:2..321692277490.145057-UTR-M-pyrifera_M_contig110662:2..32Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig110662 3..32 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815618.5888367-CDS-M-pyrifera_M_contig110662:32..4671622815618.5888367-CDS-M-pyrifera_M_contig110662:32..467Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig110662 33..467 +
1692277490.1557033-CDS-M-pyrifera_M_contig110662:32..4671692277490.1557033-CDS-M-pyrifera_M_contig110662:32..467Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig110662 33..467 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig110662.2243.1prot_M-pyrifera_M_contig110662.2243.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig110662 33..467 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig110662.2243.1

>prot_M-pyrifera_M_contig110662.2243.1 ID=prot_M-pyrifera_M_contig110662.2243.1|Name=mRNA_M-pyrifera_M_contig110662.2243.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=145bp
MIPNVRVLGPCRGDTQVELAYTDSISLGLDLPVRISGDIKGTPGCVLMGP
QGVVELDFGVIRAARHVHMSPADCAYYGCANGDSLHLRVDSPGCTTVLED
VVVRENPDVKLEVHIDTDEGNAVDLDHATSVKLFVPESCKCKHS*
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mRNA from alignment at M-pyrifera_M_contig110662:3..467+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig110662.2243.1 ID=mRNA_M-pyrifera_M_contig110662.2243.1|Name=mRNA_M-pyrifera_M_contig110662.2243.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=465bp|location=Sequence derived from alignment at M-pyrifera_M_contig110662:3..467+ (Macrocystis pyrifera P11B4 male)
ACCGTGGCGATTGTCGGCCCCCGCCGCCGGATGATTCCCAACGTTCGCGT
TCTGGGACCCTGCCGTGGCGATACTCAGGTCGAACTGGCCTATACCGATT
CCATCTCTCTCGGACTGGATCTGCCCGTCCGCATCAGTGGCGACATCAAA
GGCACACCCGGTTGTGTGCTGATGGGACCCCAGGGAGTCGTCGAACTCGA
TTTCGGCGTGATCCGGGCTGCCCGTCACGTTCACATGTCACCCGCTGACT
GTGCTTACTACGGTTGTGCCAACGGCGACAGCCTGCACCTGCGGGTCGAT
TCCCCCGGTTGCACCACGGTCCTCGAAGATGTGGTCGTACGCGAAAACCC
GGACGTCAAACTGGAAGTCCACATCGATACCGACGAAGGCAACGCCGTCG
ACCTCGATCATGCGACTTCGGTCAAACTCTTCGTCCCGGAATCCTGTAAA
TGCAAACACAGCTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig110662:3..467+

>mRNA_M-pyrifera_M_contig110662.2243.1 ID=mRNA_M-pyrifera_M_contig110662.2243.1|Name=mRNA_M-pyrifera_M_contig110662.2243.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=870bp|location=Sequence derived from alignment at M-pyrifera_M_contig110662:3..467+ (Macrocystis pyrifera P11B4 male)
ATGATTCCCAACGTTCGCGTTCTGGGACCCTGCCGTGGCGATACTCAGGT
CGAACTGGCCTATACCGATTCCATCTCTCTCGGACTGGATCTGCCCGTCC
GCATCAGTGGCGACATCAAAGGCACACCCGGTTGTGTGCTGATGGGACCC
CAGGGAGTCGTCGAACTCGATTTCGGCGTGATCCGGGCTGCCCGTCACGT
TCACATGTCACCCGCTGACTGTGCTTACTACGGTTGTGCCAACGGCGACA
GCCTGCACCTGCGGGTCGATTCCCCCGGTTGCACCACGGTCCTCGAAGAT
GTGGTCGTACGCGAAAACCCGGACGTCAAACTGGAAGTCCACATCGATAC
CGACGAAGGCAACGCCGTCGACCTCGATCATGCGACTTCGGTCAAACTCT
TCGTCCCGGAATCCTGTAAATGCAAACACAGCTGAATGATTCCCAACGTT
CGCGTTCTGGGACCCTGCCGTGGCGATACTCAGGTCGAACTGGCCTATAC
CGATTCCATCTCTCTCGGACTGGATCTGCCCGTCCGCATCAGTGGCGACA
TCAAAGGCACACCCGGTTGTGTGCTGATGGGACCCCAGGGAGTCGTCGAA
CTCGATTTCGGCGTGATCCGGGCTGCCCGTCACGTTCACATGTCACCCGC
TGACTGTGCTTACTACGGTTGTGCCAACGGCGACAGCCTGCACCTGCGGG
TCGATTCCCCCGGTTGCACCACGGTCCTCGAAGATGTGGTCGTACGCGAA
AACCCGGACGTCAAACTGGAAGTCCACATCGATACCGACGAAGGCAACGC
CGTCGACCTCGATCATGCGACTTCGGTCAAACTCTTCGTCCCGGAATCCT
GTAAATGCAAACACAGCTGA
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