mRNA_M-pyrifera_M_contig90449.20977.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig90449.20977.1
Unique NamemRNA_M-pyrifera_M_contig90449.20977.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Match: A0A7S1CII3_9STRA (Hypothetical protein n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CII3_9STRA)

HSP 1 Score: 153 bits (387), Expect = 1.940e-39
Identity = 95/202 (47.03%), Postives = 136/202 (67.33%), Query Frame = 1
Query:    1 QFASQVLLNLPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRSSLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDFELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVAQLAVGAVLGQLTAVAGIPADASLTPGAILSATSLALLVPLVGGTGPILSAVRGESLREALDTER 606
            QFA +V+ NLPP  RL +YT S++ E Q  V  + +R ++   GF Q+ + L LL  LRS  F++LYLGL++++LLVVL +LSA+L+Y+LL INV  R FELAVRR+LG  +  ++ LL+ QA +YA+PAW +GL+ A      +L     ++GIP +A LT  A+L AT L   +PLV   GPI +A+ G++LR+ALD  R
Sbjct:  340 QFAERVVFNLPPETRLPSYTHSNYDEIQAGVVDWASRLIYVL-GFSQVNAELDLLDNLRSRRFVSLYLGLILNILLVVLSALSAILIYSLLMINVQGRTFELAVRRMLGTPRAGIIFLLLVQALSYALPAWAVGLLAADAVSNVLLDSFRELSGIPLEAGLTGDAVLLATVLVASIPLVASIGPIRTAL-GKNLRDALDVTR 539          
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Match: A0A5A8C3R8_CAFRO (Uncharacterized protein n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8C3R8_CAFRO)

HSP 1 Score: 126 bits (317), Expect = 4.600e-30
Identity = 92/207 (44.44%), Postives = 130/207 (62.80%), Query Frame = 1
Query:   28 LPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRSSLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDFELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVA---------QLAVGAVLGQL-TAVAGIPADASLTPG-AILSATSLALLVPLVGGTGPILSAVRGESLREALDTERAGK 615
            LPP  R  AY        Q V+    +R +F A G   L   LPLLS + S  F+ALYL LV+ + LVVLF+L+A+++Y++LT++VA+R FE+ VRR+LGA++P +V LLV Q+  YA+P W+LGL +A         QLAV +  G+  +A +G    A +  G A+++AT+LAL VP V   GPI +AV   S+RE LD+  AGK
Sbjct:    2 LPPGERSAAYADGDVDALQAVITSIASRVVFTA-GMPSLSIDLPLLSAVSSRRFVALYLSLVLAICLVVLFALAAMVVYSILTVSVASRRFEIGVRRMLGATRPALVALLVVQSLLYALPGWILGLALAAALSGQLTQQLAVSSASGETGSATSGSATGAQVLSGSAVVAATALALAVPAVAALGPIRTAV-SRSIREGLDS--AGK 204          
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Match: L8HCM7_ACACA (Efflux ABC transporter, permease domain containing protein n=1 Tax=Acanthamoeba castellanii str. Neff TaxID=1257118 RepID=L8HCM7_ACACA)

HSP 1 Score: 119 bits (297), Expect = 2.250e-27
Identity = 76/201 (37.81%), Postives = 126/201 (62.69%), Query Frame = 1
Query:    4 FASQVLLNLPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRSSLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDFELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVAQLAVGAVLGQLTAVAGIPADASLTPGAILSATSLALLVPLVGGTGPILSAVRGESLREALDTER 606
            FA QV++NLPP R +  Y  S + + Q  V  F AR ++   GF +L + +P+   L    + +L+LGL+++V++++L  LS +L+Y+LL +NV  R FE+ V R++GA++  +V LL+ QA +YA+P+WV GL+ ++  +  V        G+P +  LTP A+  AT L + VP+V    PI  A+ G++L ++LDT+R
Sbjct:  362 FAQQVVINLPPPR-IQFYLQSDYDKIQRAVVEFSARIVYKL-GFSELLTDMPVTDSLNVTQYFSLFLGLILNVVILILLLLSTMLIYSLLMVNVETRTFEMGVYRLVGATRGGIVQLLLMQAFSYAVPSWVCGLLFSEALMMGVSVWFKEQTGVPMEPVLTPLAVFIATLLGIFVPVVSAIFPIRHAL-GQNLHDSLDTKR 559          
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Match: A0A151ZB89_9MYCE (DUF214 family protein n=1 Tax=Tieghemostelium lacteum TaxID=361077 RepID=A0A151ZB89_9MYCE)

HSP 1 Score: 113 bits (283), Expect = 1.670e-25
Identity = 75/203 (36.95%), Postives = 131/203 (64.53%), Query Frame = 1
Query:    4 FASQVLLNLPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRSSLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDFELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVAQL---AVGAVLGQLTAVAGIPADASLTPGAILSATSLALLVPLVGGTGPILSAVRGESLREALDTE 603
            FA  +++NLPPSR L  YT S+  E    + +F ++ ++   GF  L SSLP+++ L  + ++AL+LGL+++V++ +L  LS LL+Y+LL I+V  R FE+ V R++G ++  ++ L++ +A +Y+IP+W +GLV AQL    V A+   +T   G+P    L+P ++L A+ L  L+P+     PI +A+ G++L ++LD +
Sbjct:  360 FAQSIVINLPPSR-LEPYTNSNQDEILQYLIKFSSKILYKI-GFNLLDSSLPIMNVLSKNRYVALFLGLILNVIIFILXXLSILLIYSLLMIDVETRSFEMGVMRMIGTTRSGIIQLMLFKAFSYSIPSWAIGLVCAQLFGFVVSAIFKNIT---GVPIPTKLSPESVLLASLLGFLIPIAASILPIRNAL-GKNLHDSLDVK 556          
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Match: A0A0L0DGR6_THETB (FtsX domain-containing protein n=1 Tax=Thecamonas trahens ATCC 50062 TaxID=461836 RepID=A0A0L0DGR6_THETB)

HSP 1 Score: 111 bits (277), Expect = 9.760e-25
Identity = 77/202 (38.12%), Postives = 123/202 (60.89%), Query Frame = 1
Query:    1 QFASQVLLNLPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRSSLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDFELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVAQLAVGAVLGQLTAVAGIPADASLTPGAILSATSLALLVPLVGGTGPILSAVRGESLREALDTER 606
            +FAS++ +NLPP R +  Y +S F   Q  V RF ++ ++A  GF QL +  P++  LR+  F +L+LGL++++++ +L  LS LL+Y+LL +NV  R FE+   R+LG ++  VV L + QA AYA+PA+V+G+ V+QLA   +     +   +     L+P  I+ AT L L +P++    PI +A+ G SL  AL   R
Sbjct:  390 EFASEINVNLPPPR-IDPYLSSDFDTIQKNVLRFTSKVVYAL-GFNQLDADEPIVENLRATRFFSLFLGLILNIVIFILSGLSVLLIYSLLMVNVETRTFEMGTMRMLGMTKAGVVQLFLLQAFAYALPAYVVGMGVSQLAFYLLANLFESSTDVSISPWLSPAGIILATVLGLTIPVISALAPIRTAL-GVSLVAALQVSR 588          
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Match: Q54MU0_DICDI (FtsX domain-containing protein n=1 Tax=Dictyostelium discoideum TaxID=44689 RepID=Q54MU0_DICDI)

HSP 1 Score: 109 bits (273), Expect = 3.650e-24
Identity = 70/200 (35.00%), Postives = 128/200 (64.00%), Query Frame = 1
Query:    4 FASQVLLNLPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRSSLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDFELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVAQLAVGAVLGQLTAVAGIPADASLTPGAILSATSLALLVPLVGGTGPILSAVRGESLREALDTE 603
            ++ QV++NLPPSR L  Y  S+       +  F  + ++   GF +L S LP++S L  + ++AL+LGL+++V++ +L  LS LL+Y+LL I+V  R FE+ V R++G ++  ++ L++ +A +Y++P+W LGL++AQ+    V     ++ G+P    LT  AIL A++L +++P+     PI +A+ G++L ++LD +
Sbjct:  421 YSKQVIINLPPSR-LTPYINSNQDVILEKIVDFSNKILYKV-GFNELSSELPVMSELSKNRYVALFLGLILNVIIFILXXLSILLIYSLLMIDVETRTFEMGVMRMIGTTRNGIIQLMLFKAFSYSVPSWGLGLLIAQVFGFIVSAIFKSITGVPIPPKLTGEAILLASALGIIIPIASAIFPIRNAL-GKNLHDSLDVK 617          
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Match: D2VH74_NAEGR (Predicted protein n=1 Tax=Naegleria gruberi TaxID=5762 RepID=D2VH74_NAEGR)

HSP 1 Score: 109 bits (272), Expect = 4.870e-24
Identity = 75/205 (36.59%), Postives = 125/205 (60.98%), Query Frame = 1
Query:    4 FASQVLLNLPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRSSLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDFELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVAQLAVGAVLGQLTAVAGIPADASLTPGAILSATSLALLVPLVGGTGPILSAVRGESLREALDTERAGKA 618
            +AS+V +N PP+R L AY  + +   +  + +F + +M+   GF QL SS P+L  +    F AL++GL++ ++L+VL  LS +L+Y+LL INV  R +E+ + R++G  +  +V L++ QA  Y IPAW+LGL++ QL+   V   L+          ++  A   AT L L VP++    PI +A+ G++L ++LDT+R+G A
Sbjct:  281 YASEVYVNYPPNR-LTAYNENDYKVIREKITKFASTSMYLM-GFNQLSSSFPILEMMEVTQFFALFVGLIISIILIVLCLLSVVLIYSLLMINVENRRYEMGLLRMIGLKRRNLVELILVQAMWYGIPAWILGLIIGQLSYSVVAILLSTQLEANVSYIVSGEAFALATFLGLTVPVLSAILPIKTAL-GQNLHDSLDTQRSGTA 482          
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Match: F0ZQK2_DICPU (Uncharacterized protein n=1 Tax=Dictyostelium purpureum TaxID=5786 RepID=F0ZQK2_DICPU)

HSP 1 Score: 107 bits (266), Expect = 3.130e-23
Identity = 69/201 (34.33%), Postives = 127/201 (63.18%), Query Frame = 1
Query:    1 QFASQVLLNLPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRSSLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDFELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVAQLAVGAVLGQLTAVAGIPADASLTPGAILSATSLALLVPLVGGTGPILSAVRGESLREALDTE 603
            ++   +++NLPPSR L  Y  S+       +  F  + ++   GF +  SSLP++ +L  + ++AL+LGL+++V++ +L  LS LL+Y+LL I+V  R FE+ V R++G ++  ++ L++ +A +Y++P+W LGL+VAQ+    V     ++ G+P    LT  +IL AT+L +++P+     PI SA+ G++L ++LD +
Sbjct:  394 EYTQIMIVNLPPSR-LEPYINSNQDTILQHIVDFSNKILYKI-GFNEFSSSLPVMGQLSKNRYVALFLGLILNVIIFILXXLSILLIYSLLMIDVETRTFEMGVMRMIGTTRNGIIQLMLFKAFSYSVPSWALGLLVAQIFGFVVSAIFKSITGVPIPTRLTGESILLATALGIIIPIASAIFPIRSAL-GKNLHDSLDVK 591          
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Match: A0A8J4PT26_9MYCE (Uncharacterized protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4PT26_9MYCE)

HSP 1 Score: 105 bits (261), Expect = 1.460e-22
Identity = 66/198 (33.33%), Postives = 126/198 (63.64%), Query Frame = 1
Query:    4 FASQVLLNLPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRSSLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDFELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVAQLAVGAVLGQLTAVAGIPADASLTPGAILSATSLALLVPLVGGTGPILSAVRGESLREALD 597
            ++  +++N PPSR L  Y  S+       +  F +  ++   GF QL +SLP++  L  + ++AL+LGL+++V++ +L  LS LL+Y+LL I+V  R FE+ V R++G ++  ++ L++ +A +Y++P+WVLGL++AQ+    V     ++ G+P    LT  A++ +T L +++P+     PI +A+ G++L+++LD
Sbjct:  410 YSQIMIINFPPSR-LEPYIDSNQDHVLEKLNLFSSNILYKI-GFNQLDTSLPIMDELSKNRYVALFLGLILNVIIFILXXLSILLIYSLLMIDVETRTFEMGVMRMIGTTRSGIIQLMLFKAFSYSLPSWVLGLIMAQIFGVIVSVWFKSITGVPIPTKLTGEAVILSTLLGMIIPVASAIFPIRNAL-GKNLQDSLD 604          
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Match: A0A6A5BWZ4_NAEFO (Uncharacterized protein n=2 Tax=Naegleria TaxID=5761 RepID=A0A6A5BWZ4_NAEFO)

HSP 1 Score: 103 bits (256), Expect = 6.910e-22
Identity = 71/201 (35.32%), Postives = 121/201 (60.20%), Query Frame = 1
Query:    4 FASQVLLNLPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRSSLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDFELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVAQLAVGAVLGQLTAVAGIPADASLTPGAILSATSLALLVPLVGGTGPILSAVRGESLREALDTER 606
            +A  V  N PP+R L AY  + F + +  +  F + AM+   GF Q+ +  P+L +++   F AL++GL++ ++LVVL  LS +L+Y+LL INV  R FE+ + R+LG  +  +V L++ QA  Y IPAWV+GL++ Q++   V   L++          +  A+  A+ L + VP++    PI +A+ G++L ++LDT+R
Sbjct:  515 YAGYVYFNYPPNR-LTAYNENDFKKIRDKITDFSSLAMYLI-GFNQVETDFPILEQMQQTQFFALFVGLIISIILVVLSLLSVVLIYSLLMINVENRRFEMGLLRILGLKRIHLVELILVQAMWYGIPAWVIGLIIGQVSYAGVAMLLSSTLEARVSFFASFEAVAFASLLGIGVPIIASILPIKTAL-GQNLHDSLDTQR 712          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig90449.20977.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S1CII3_9STRA1.940e-3947.03Hypothetical protein n=1 Tax=Bicosoecida sp. CB-20... [more]
A0A5A8C3R8_CAFRO4.600e-3044.44Uncharacterized protein n=3 Tax=Cafeteria roenberg... [more]
L8HCM7_ACACA2.250e-2737.81Efflux ABC transporter, permease domain containing... [more]
A0A151ZB89_9MYCE1.670e-2536.95DUF214 family protein n=1 Tax=Tieghemostelium lact... [more]
A0A0L0DGR6_THETB9.760e-2538.12FtsX domain-containing protein n=1 Tax=Thecamonas ... [more]
Q54MU0_DICDI3.650e-2435.00FtsX domain-containing protein n=1 Tax=Dictyosteli... [more]
D2VH74_NAEGR4.870e-2436.59Predicted protein n=1 Tax=Naegleria gruberi TaxID=... [more]
F0ZQK2_DICPU3.130e-2334.33Uncharacterized protein n=1 Tax=Dictyostelium purp... [more]
A0A8J4PT26_9MYCE1.460e-2233.33Uncharacterized protein n=1 Tax=Polysphondylium vi... [more]
A0A6A5BWZ4_NAEFO6.910e-2235.32Uncharacterized protein n=2 Tax=Naegleria TaxID=57... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig90449contigM-pyrifera_M_contig90449:22..639 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score109.0
Seed ortholog evalue2.7e-21
Seed eggNOG ortholog529818.AMSG_00684T0
Hectar predicted targeting categorysignal anchor
EggNOG free text desc.FtsX-like permease family
EggNOG OGs2BZJY@1,2QQQ7@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size618
Cds size618
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816816.7272844-CDS-M-pyrifera_M_contig90449:21..6391622816816.7272844-CDS-M-pyrifera_M_contig90449:21..639Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90449 22..639 -
1692278195.5145817-CDS-M-pyrifera_M_contig90449:21..6391692278195.5145817-CDS-M-pyrifera_M_contig90449:21..639Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90449 22..639 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig90449.20977.1prot_M-pyrifera_M_contig90449.20977.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig90449 22..639 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig90449.20977.1

>prot_M-pyrifera_M_contig90449.20977.1 ID=prot_M-pyrifera_M_contig90449.20977.1|Name=mRNA_M-pyrifera_M_contig90449.20977.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=206bp
QFASQVLLNLPPSRRLLAYTASSFSETQTVVGRFGARAMFAARGFGQLRS
SLPLLSRLRSDAFLALYLGLVMDVLLVVLFSLSALLLYALLTINVAARDF
ELAVRRVLGASQPVVVLLLVQQAAAYAIPAWVLGLVVAQLAVGAVLGQLT
AVAGIPADASLTPGAILSATSLALLVPLVGGTGPILSAVRGESLREALDT
ERAGKA
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mRNA from alignment at M-pyrifera_M_contig90449:22..639-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig90449.20977.1 ID=mRNA_M-pyrifera_M_contig90449.20977.1|Name=mRNA_M-pyrifera_M_contig90449.20977.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=618bp|location=Sequence derived from alignment at M-pyrifera_M_contig90449:22..639- (Macrocystis pyrifera P11B4 male)
CAATTCGCCTCCCAAGTGCTTCTCAACCTGCCCCCTTCTCGTCGGCTCTT GGCCTACACGGCGTCTTCGTTCTCTGAGACCCAGACGGTTGTGGGTCGAT TCGGGGCGAGGGCAATGTTTGCTGCTCGTGGCTTTGGCCAACTTCGGTCC TCCTTGCCTCTCCTCTCCCGTCTTCGAAGTGATGCATTTTTGGCTCTCTA CTTGGGTCTGGTGATGGACGTGCTGCTGGTGGTGCTGTTTTCGCTGTCTG CGCTGCTGCTCTATGCGCTGCTGACCATCAACGTGGCAGCTCGGGATTTT GAACTGGCGGTGCGACGGGTGCTGGGGGCGTCTCAGCCAGTGGTGGTGTT GTTGTTGGTGCAGCAAGCAGCAGCCTATGCGATTCCAGCGTGGGTGCTGG GTCTGGTGGTGGCGCAATTGGCAGTGGGAGCAGTGCTGGGCCAACTCACT GCCGTCGCAGGCATTCCAGCAGACGCATCTCTCACCCCAGGCGCAATCCT CTCCGCCACATCCCTAGCTCTCCTCGTGCCTCTTGTCGGAGGCACCGGTC CCATTCTCTCCGCTGTGCGAGGAGAATCGCTTCGAGAAGCGCTCGACACC GAACGGGCTGGAAAAGCA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig90449:22..639-

>mRNA_M-pyrifera_M_contig90449.20977.1 ID=mRNA_M-pyrifera_M_contig90449.20977.1|Name=mRNA_M-pyrifera_M_contig90449.20977.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1236bp|location=Sequence derived from alignment at M-pyrifera_M_contig90449:22..639- (Macrocystis pyrifera P11B4 male)
CAATTCGCCTCCCAAGTGCTTCTCAACCTGCCCCCTTCTCGTCGGCTCTT
GGCCTACACGGCGTCTTCGTTCTCTGAGACCCAGACGGTTGTGGGTCGAT
TCGGGGCGAGGGCAATGTTTGCTGCTCGTGGCTTTGGCCAACTTCGGTCC
TCCTTGCCTCTCCTCTCCCGTCTTCGAAGTGATGCATTTTTGGCTCTCTA
CTTGGGTCTGGTGATGGACGTGCTGCTGGTGGTGCTGTTTTCGCTGTCTG
CGCTGCTGCTCTATGCGCTGCTGACCATCAACGTGGCAGCTCGGGATTTT
GAACTGGCGGTGCGACGGGTGCTGGGGGCGTCTCAGCCAGTGGTGGTGTT
GTTGTTGGTGCAGCAAGCAGCAGCCTATGCGATTCCAGCGTGGGTGCTGG
GTCTGGTGGTGGCGCAATTGGCAGTGGGAGCAGTGCTGGGCCAACTCACT
GCCGTCGCAGGCATTCCAGCAGACGCATCTCTCACCCCAGGCGCAATCCT
CTCCGCCACATCCCTAGCTCTCCTCGTGCCTCTTGTCGGAGGCACCGGTC
CCATTCTCTCCGCTGTGCGAGGAGAATCGCTTCGAGAAGCGCTCGACACC
GAACGGGCTGGAAAAGCACAATTCGCCTCCCAAGTGCTTCTCAACCTGCC
CCCTTCTCGTCGGCTCTTGGCCTACACGGCGTCTTCGTTCTCTGAGACCC
AGACGGTTGTGGGTCGATTCGGGGCGAGGGCAATGTTTGCTGCTCGTGGC
TTTGGCCAACTTCGGTCCTCCTTGCCTCTCCTCTCCCGTCTTCGAAGTGA
TGCATTTTTGGCTCTCTACTTGGGTCTGGTGATGGACGTGCTGCTGGTGG
TGCTGTTTTCGCTGTCTGCGCTGCTGCTCTATGCGCTGCTGACCATCAAC
GTGGCAGCTCGGGATTTTGAACTGGCGGTGCGACGGGTGCTGGGGGCGTC
TCAGCCAGTGGTGGTGTTGTTGTTGGTGCAGCAAGCAGCAGCCTATGCGA
TTCCAGCGTGGGTGCTGGGTCTGGTGGTGGCGCAATTGGCAGTGGGAGCA
GTGCTGGGCCAACTCACTGCCGTCGCAGGCATTCCAGCAGACGCATCTCT
CACCCCAGGCGCAATCCTCTCCGCCACATCCCTAGCTCTCCTCGTGCCTC
TTGTCGGAGGCACCGGTCCCATTCTCTCCGCTGTGCGAGGAGAATCGCTT
CGAGAAGCGCTCGACACCGAACGGGCTGGAAAAGCA
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