mRNA_M-pyrifera_M_contig90208.20916.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig90208.20916.1
Unique NamemRNA_M-pyrifera_M_contig90208.20916.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Match: A0A2E2NAG7_9BACT (Preprotein translocase subunit SecE n=1 Tax=Phycisphaerae bacterium TaxID=2026778 RepID=A0A2E2NAG7_9BACT)

HSP 1 Score: 131 bits (330), Expect = 9.080e-35
Identity = 82/194 (42.27%), Postives = 116/194 (59.79%), Query Frame = 1
Query:    1 AVRLPVRSWTLATNGTQGEASPGDSINLYKYSEQALDEEYEVFGTALVESFESGQGGNAKLVINSFSSPEAAKRGGETLRVAIEQPNQAATMNAQVVGASSISIFPVLYLQAGIAGAILLLGAIALYYFVGASRKSVGFLIATDGEMKKVNWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQ 582
            A  LPV ++T   +   G+ +PG ++ L   S    D  Y   GT ++E+ E     +A++ + S +    A    +   V        A   A+V    +  +FP +YLQA +AGAI+L+GA+ALY+FVGA+ KS  FLIATDGEM+KVNW++ RE+RGSTIVVI A FLIA  L+ +D  F + F AIGVL+
Sbjct:   39 AFDLPVAAYTYRVSNAAGDIAPGQTLELRDLSLDGSDT-YVTIGTGVIENVEQINTEDARVRLGSIAP---ANEDADVTSVKRLAGAAGAPYAARVESFDTHRVFPPVYLQAAVAGAIILIGAVALYWFVGANPKSCEFLIATDGEMRKVNWSTPREIRGSTIVVIVAAFLIAAILWIIDLGFQQTFDAIGVLE 228          
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Match: A0A3M2CHN9_9BACT (Preprotein translocase subunit SecE n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3M2CHN9_9BACT)

HSP 1 Score: 126 bits (317), Expect = 7.090e-34
Identity = 68/139 (48.92%), Postives = 93/139 (66.91%), Query Frame = 1
Query:  169 GNAKLVINSFSSPEAAKRGGETLRVAIEQPNQAATMNAQVVGASSISIFPVLYLQAGIAGAILLLGAIALYYFVGASRKSVGFLIATDGEMKKVNWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQK 585
            G A LV++SF+S +      +T+RV +          A V GA++  IFP LYLQAG+AG I+L+G +A+ +FV  + +S  FLIATD EMKKVNW++Y++++GSTIVVI A FLIAG LFG+D   +  F  I VLQ 
Sbjct:    4 GRADLVVDSFNSTKVRDEAADTVRVIVGDDPLNPAFTATVDGATAEPIFPQLYLQAGVAGTIILIGTLAIVWFVAINPRSSDFLIATDSEMKKVNWSTYKQIKGSTIVVIVAAFLIAGILFGIDMSLSWFFRTIDVLQS 142          
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Match: A0A7D8YU50_9BACT (Protein translocase subunit SecE n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7D8YU50_9BACT)

HSP 1 Score: 127 bits (319), Expect = 2.860e-33
Identity = 88/205 (42.93%), Postives = 114/205 (55.61%), Query Frame = 1
Query:    4 VRLPVRSWTLATNGTQGEAS-PGDSINLYKYSE-QALDEEYEVFGTALVESFESGQGGNAKLVINSFS---------SPEAAKRGGETLRVAIEQPNQAATMNAQVVGASSISIFPVLYLQAGIAGAILLLGAIALYYFVGASRKSVGFLIATDGEMKKVNWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQK 585
            VRLP  +W L   G QGE    G  + L   S  + LD       TA VES+  G  G   LV+   +         +    + GG T   A+  P    T+      A  I IF   YLQAG AG +L+LGA  +Y+ VG  RK+V FLIATDGEMKKVNW++ +EV GSTIVV+ ATFLIAG LF +D +F+  F++ GVL++
Sbjct:   24 VRLPTPTWQLDVVGLQGEPPVAGQGVELLDASGVETLD-----VATATVESWSPGTRGTGTLVVRGVTLADRDFELVNANTFRAGGAT---ALGTPASEGTVRT----ARGIPIFEPTYLQAGGAGIVLILGAALIYWLVGVKRKTVEFLIATDGEMKKVNWSTRKEVTGSTIVVVVATFLIAGVLFVIDMVFSYAFASAGVLER 216          
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Match: A0A2G1ZS76_9BACT (Preprotein translocase subunit SecE n=1 Tax=Phycisphaera sp. TaxID=2030824 RepID=A0A2G1ZS76_9BACT)

HSP 1 Score: 127 bits (318), Expect = 9.400e-33
Identity = 79/195 (40.51%), Postives = 121/195 (62.05%), Query Frame = 1
Query:    7 RLPVRSWTLATNGTQGEASPGDSINLYKYSEQALDEEYEVFGTALVESFESGQGGNAKLVINSFSSPEAAKRGGETLRVAIEQ-PNQAATMNAQVVGASSISI--FPVLYLQAGIAGAILLLGAIALYYFVGASRKSVGFLIATDGEMKKVNWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQ 582
            R P  +W L     +G  SPG  +++Y  +    DE   + GTA+V+SF  GQ    ++ +++    +  +   E  +V +E    Q +  +A+V   ++  I  F V+Y+QAGIAGA+LL+G++  + FVGA R++V FLIATDGEMKKVNW++ +EV+GST+VV+ A+F++A  +F VD  F   F  IGVLQ
Sbjct:   59 RPPTPTWKLQLVTIEGTLSPGVQVDIYDMT----DEGPVLMGTAVVDSFTKGQARTGEVEVSNIQL-QGDRSLVEADQVVVEDIMGQDSPFSARVPAGTAFGIPAFEVIYVQAGIAGAVLLIGSLVTFIFVGAKRRTVDFLIATDGEMKKVNWSTRKEVQGSTVVVVIASFMLASGIFVVDYGFGAFFKLIGVLQ 248          
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Match: A0A3D4UJD7_9BACT (Protein translocase subunit SecE n=2 Tax=Phycisphaerae TaxID=666505 RepID=A0A3D4UJD7_9BACT)

HSP 1 Score: 111 bits (278), Expect = 8.090e-29
Identity = 56/77 (72.73%), Postives = 68/77 (88.31%), Query Frame = 1
Query:  355 LLLGAIALYYFVGASRKSVGFLIATDGEMKKVNWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQK 585
            +LLGAIALY FVGA++K+V FL++TDGEMKKVNWTSY+EV+GST+VVI ATFLIAGFLF VDT F+  FS I VL++
Sbjct:    1 MLLGAIALYIFVGANKKTVEFLVSTDGEMKKVNWTSYKEVKGSTMVVIAATFLIAGFLFIVDTAFSSFFSFINVLER 77          
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Match: A0A3M2BJU0_9BACT (Protein translocase subunit SecE n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A3M2BJU0_9BACT)

HSP 1 Score: 116 bits (290), Expect = 8.520e-29
Identity = 75/196 (38.27%), Postives = 109/196 (55.61%), Query Frame = 1
Query:    4 VRLPVRSWTLATNGTQG-EASPGDSINLYKYSEQALDEEYEVFGTALVESFESGQGGNAKLVINSFSSPEAAKRGGETLRVA--IEQPNQAATMNAQVVGASSISIFPVLYLQAGIAGAILLLGAIALYYFVGASRKSVGFLIATDGEMKKVNWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQ 582
            + LP + W L+   T   + + G  + L +   Q  D  YE  G+ALVES+         L I          R G T  +A  +   + A++    +   + I + PVLYLQA +AG+I+ +GA+ +Y+ VG   ++V FL+ATDGEMKKVNW++ +E+ GST VVI A  LIAG LF +D  F+  F  IGVL+
Sbjct:   40 IGLPAKGWELSVTVTSAPDLAEGSFVVLER---QTGDGTYERVGSALVESYTPATQTRGTLTIRQVEMD----REGLTPNIAGRVRAEDAASSFVGTITNKTPIPLMPVLYLQAAVAGSIIFIGALCIYWLVGVKPETVDFLVATDGEMKKVNWSTRKEIIGSTQVVIVAAVLIAGILFFIDLAFSNFFKFIGVLE 228          
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Match: A0A5M4D950_9CYAN (Protein translocase subunit SecE n=1 Tax=Cyanobacteria bacterium CYA TaxID=2484221 RepID=A0A5M4D950_9CYAN)

HSP 1 Score: 115 bits (287), Expect = 2.380e-28
Identity = 79/193 (40.93%), Postives = 103/193 (53.37%), Query Frame = 1
Query:    4 VRLPVRSWTLATNGTQGEASPGDSINLYKYSEQALDEEYEVFGTALVESFESGQGGNAKLVINSFSSPEAAKRGGETLRVAIEQPNQAATMNAQVVGASSISIFPVLYLQAGIAGAILLLGAIALYYFVGASRKSVGFLIATDGEMKKVNWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQ 582
            + LPV+ + L  N T G+  P     +      A D    V  TA VES      G   L IN     +      +  RV  E    +   + QVVG   I +FP LY+QA IAG I+L+G   LY+ VG    +V FL+ATDGEMKKVNW++ +E+ GST VVI A+ LIAG LF +D  F+  F  IGVL+
Sbjct:   40 ITLPVKGYVLTLNVT-GDQQPAPQSGVIIERVDARDAVRPV-ATAQVESLTVSGTGRGVLAINHIEFAKGELGIPQDARVRTE--GDSLNFSGQVVGREQIDLFPRLYVQATIAGVIILVGTACLYWLVGTKPGTVDFLVATDGEMKKVNWSTRKEIIGSTQVVIVASVLIAGILFVIDLAFSNFFKLIGVLE 228          
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Match: A0A7V1M085_9BACT (Protein translocase subunit SecE n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7V1M085_9BACT)

HSP 1 Score: 112 bits (280), Expect = 3.700e-28
Identity = 71/157 (45.22%), Postives = 100/157 (63.69%), Query Frame = 1
Query:  130 GTALVESFESGQGGNAKLVINSFS-SPEAAKRGGETLRVAIEQPN-----QAATMNAQVVGASSISIFPVLYLQAGIAGAILLLGAIALYYFVGASRKSVGFLIATDGEMKKVNWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQ 582
            GTA ++S+  G+     +++++    P+ +    E  RV  E P+     Q+     Q  GA  I  F V+YLQA +AG ILLLG+I  Y FVG+  KSV FLIATDGEMKKVNW++ +E++GST VV+ A+FL+A  +F VD  F ++F A+GVLQ
Sbjct:    2 GTATIDSYTPGENEKGAIIVSNIHLDPDRSMV--EVRRV--EYPDMIGGGQSFGAEVQHNGAFGIPAFDVIYLQAAVAGTILLLGSIGTYLFVGSIPKSVDFLIATDGEMKKVNWSTRKEIQGSTAVVVIASFLLAIMIFFVDLGFQQIFRAVGVLQ 154          
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Match: A0A7Z9U9T9_9BACT (Protein translocase subunit SecE n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7Z9U9T9_9BACT)

HSP 1 Score: 107 bits (267), Expect = 3.690e-27
Identity = 56/77 (72.73%), Postives = 63/77 (81.82%), Query Frame = 1
Query:  355 LLLGAIALYYFVGASRKSVGFLIATDGEMKKVNWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQK 585
            +L+GAIA Y FVG  + +V FLIATDGEMKKVNWTSY+EV+GSTIVVI ATFLIAGFLF VD  F+  FS I VLQK
Sbjct:    1 MLIGAIATYIFVGVKKSAVEFLIATDGEMKKVNWTSYKEVKGSTIVVIAATFLIAGFLFVVDLAFSNFFSWIDVLQK 77          
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Match: A0A7Y5VXP9_9BACT (Protein translocase subunit SecE n=1 Tax=Phycisphaerales bacterium TaxID=2052180 RepID=A0A7Y5VXP9_9BACT)

HSP 1 Score: 109 bits (272), Expect = 3.670e-26
Identity = 76/198 (38.38%), Postives = 106/198 (53.54%), Query Frame = 1
Query:    1 AVRLPVRSWTLATNGTQGEASPGDSINLYKYSEQALDEEYEVFGTALVESFESGQGGNAKLVINS---FSSPEAAKRGGETLRVAIEQPNQAATMNAQVVGASSISIFPVLYLQAGIAGAILLLGAIALYYFVGASRKSVGFLIATDGEMKKVNWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQK 585
            A+ +P++SW L+            +++L   S         + G+A + SFE        L I      S+ E A  GG+ + +  ++          V  A  + +FP+LYLQAG+A AIL++G   +Y FVG   KS  FLIATDGEMKKV+W++ REV GST VVI A FLIAG L+  D  F  VF +I +L K
Sbjct:   37 AIPIPMQSWVLSLKNMTEAPDATGTVDLMDESPGTGPLAVRI-GSATIVSFEKAGDNLGNLTIKEPQMVSNHEPADVGGQAVLIGGKRYT--------VATAVGVPLFPILYLQAGVAAAILIIGVALIYTFVGVKPKSAEFLIATDGEMKKVHWSTRREVFGSTWVVIAACFLIAGLLWVFDFGFVTVFQSIKLLHK 225          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig90208.20916.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2E2NAG7_9BACT9.080e-3542.27Preprotein translocase subunit SecE n=1 Tax=Phycis... [more]
A0A3M2CHN9_9BACT7.090e-3448.92Preprotein translocase subunit SecE n=1 Tax=Planct... [more]
A0A7D8YU50_9BACT2.860e-3342.93Protein translocase subunit SecE n=1 Tax=Phycispha... [more]
A0A2G1ZS76_9BACT9.400e-3340.51Preprotein translocase subunit SecE n=1 Tax=Phycis... [more]
A0A3D4UJD7_9BACT8.090e-2972.73Protein translocase subunit SecE n=2 Tax=Phycispha... [more]
A0A3M2BJU0_9BACT8.520e-2938.27Protein translocase subunit SecE n=1 Tax=Planctomy... [more]
A0A5M4D950_9CYAN2.380e-2840.93Protein translocase subunit SecE n=1 Tax=Cyanobact... [more]
A0A7V1M085_9BACT3.700e-2845.22Protein translocase subunit SecE n=1 Tax=Phycispha... [more]
A0A7Z9U9T9_9BACT3.690e-2772.73Protein translocase subunit SecE n=1 Tax=Phycispha... [more]
A0A7Y5VXP9_9BACT3.670e-2638.38Protein translocase subunit SecE n=1 Tax=Phycispha... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig90208contigM-pyrifera_M_contig90208:5..592 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score62.0
Seed ortholog evalue3.6e-07
Seed eggNOG ortholog1142394.PSMK_08330
Preferred namesecE
KEGG koko:K03073
KEGG TC3.A.5.1,3.A.5.2
KEGG Pathwayko02024,ko03060,ko03070,map02024,map03060,map03070
KEGG ModuleM00335
Hectar predicted targeting categoryother localisation
GOsGO:0002790,GO:0003674,GO:0005215,GO:0005575,GO:0005623,GO:0005886,GO:0005887,GO:0006605,GO:0006612,GO:0006613,GO:0006614,GO:0006616,GO:0006810,GO:0006886,GO:0008104,GO:0008150,GO:0008320,GO:0008565,GO:0009306,GO:0009987,GO:0015031,GO:0015833,GO:0016020,GO:0016021,GO:0016043,GO:0022857,GO:0022884,GO:0031224,GO:0031226,GO:0031522,GO:0032940,GO:0032978,GO:0032991,GO:0033036,GO:0033365,GO:0034613,GO:0042886,GO:0042887,GO:0043952,GO:0044425,GO:0044459,GO:0044464,GO:0045047,GO:0045184,GO:0046903,GO:0046907,GO:0051179,GO:0051205,GO:0051234,GO:0051641,GO:0051649,GO:0055085,GO:0061024,GO:0065002,GO:0070727,GO:0070972,GO:0071702,GO:0071705,GO:0071806,GO:0071840,GO:0071944,GO:0072594,GO:0072599,GO:0072657,GO:0090150,GO:1904680
EggNOG free text desc.Essential subunit of the Sec protein translocation channel SecYEG. Clamps together the 2 halves of SecY. May contact the channel plug during translocation
EggNOG OGs2J0E7@203682,COG0690@1,COG0690@2
COG Functional cat.U
Best tax levelPlanctomycetes
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko02044
Exons1
Model size588
Cds size588
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816813.8209784-CDS-M-pyrifera_M_contig90208:4..5921622816813.8209784-CDS-M-pyrifera_M_contig90208:4..592Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90208 5..592 +
1692278193.9095874-CDS-M-pyrifera_M_contig90208:4..5921692278193.9095874-CDS-M-pyrifera_M_contig90208:4..592Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig90208 5..592 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig90208.20916.1prot_M-pyrifera_M_contig90208.20916.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig90208 5..592 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig90208.20916.1

>prot_M-pyrifera_M_contig90208.20916.1 ID=prot_M-pyrifera_M_contig90208.20916.1|Name=mRNA_M-pyrifera_M_contig90208.20916.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=196bp
AVRLPVRSWTLATNGTQGEASPGDSINLYKYSEQALDEEYEVFGTALVES
FESGQGGNAKLVINSFSSPEAAKRGGETLRVAIEQPNQAATMNAQVVGAS
SISIFPVLYLQAGIAGAILLLGAIALYYFVGASRKSVGFLIATDGEMKKV
NWTSYREVRGSTIVVITATFLIAGFLFGVDTIFAKVFSAIGVLQK*
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mRNA from alignment at M-pyrifera_M_contig90208:5..592+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig90208.20916.1 ID=mRNA_M-pyrifera_M_contig90208.20916.1|Name=mRNA_M-pyrifera_M_contig90208.20916.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=588bp|location=Sequence derived from alignment at M-pyrifera_M_contig90208:5..592+ (Macrocystis pyrifera P11B4 male)
GCGGTCCGTTTGCCTGTCCGATCGTGGACCCTTGCGACGAACGGGACCCA GGGTGAAGCTTCCCCGGGAGACTCGATCAATCTCTACAAATACTCTGAGC AGGCGCTCGACGAAGAGTATGAAGTATTCGGCACAGCGCTCGTCGAGTCC TTCGAGTCTGGTCAGGGCGGCAACGCAAAGCTCGTGATCAACTCGTTCAG CTCTCCCGAAGCTGCGAAGCGAGGGGGCGAGACGCTTCGCGTCGCGATCG AGCAGCCCAATCAAGCTGCGACGATGAACGCGCAAGTCGTCGGCGCATCG AGCATCTCGATCTTCCCAGTCCTGTATCTGCAGGCAGGCATCGCGGGCGC GATCCTGCTGCTCGGCGCGATCGCACTGTACTACTTTGTCGGCGCAAGCC GCAAGTCTGTCGGCTTCCTTATCGCGACTGATGGGGAGATGAAAAAGGTC AACTGGACTTCCTATCGCGAAGTGCGCGGCTCGACGATTGTCGTCATTAC CGCGACTTTCTTGATCGCTGGATTCCTCTTTGGTGTAGACACGATCTTCG CGAAAGTGTTCTCTGCGATCGGCGTCCTTCAGAAATAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig90208:5..592+

>mRNA_M-pyrifera_M_contig90208.20916.1 ID=mRNA_M-pyrifera_M_contig90208.20916.1|Name=mRNA_M-pyrifera_M_contig90208.20916.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1176bp|location=Sequence derived from alignment at M-pyrifera_M_contig90208:5..592+ (Macrocystis pyrifera P11B4 male)
GCGGTCCGTTTGCCTGTCCGATCGTGGACCCTTGCGACGAACGGGACCCA
GGGTGAAGCTTCCCCGGGAGACTCGATCAATCTCTACAAATACTCTGAGC
AGGCGCTCGACGAAGAGTATGAAGTATTCGGCACAGCGCTCGTCGAGTCC
TTCGAGTCTGGTCAGGGCGGCAACGCAAAGCTCGTGATCAACTCGTTCAG
CTCTCCCGAAGCTGCGAAGCGAGGGGGCGAGACGCTTCGCGTCGCGATCG
AGCAGCCCAATCAAGCTGCGACGATGAACGCGCAAGTCGTCGGCGCATCG
AGCATCTCGATCTTCCCAGTCCTGTATCTGCAGGCAGGCATCGCGGGCGC
GATCCTGCTGCTCGGCGCGATCGCACTGTACTACTTTGTCGGCGCAAGCC
GCAAGTCTGTCGGCTTCCTTATCGCGACTGATGGGGAGATGAAAAAGGTC
AACTGGACTTCCTATCGCGAAGTGCGCGGCTCGACGATTGTCGTCATTAC
CGCGACTTTCTTGATCGCTGGATTCCTCTTTGGTGTAGACACGATCTTCG
CGAAAGTGTTCTCTGCGATCGGCGTCCTTCAGAAATAAGCGGTCCGTTTG
CCTGTCCGATCGTGGACCCTTGCGACGAACGGGACCCAGGGTGAAGCTTC
CCCGGGAGACTCGATCAATCTCTACAAATACTCTGAGCAGGCGCTCGACG
AAGAGTATGAAGTATTCGGCACAGCGCTCGTCGAGTCCTTCGAGTCTGGT
CAGGGCGGCAACGCAAAGCTCGTGATCAACTCGTTCAGCTCTCCCGAAGC
TGCGAAGCGAGGGGGCGAGACGCTTCGCGTCGCGATCGAGCAGCCCAATC
AAGCTGCGACGATGAACGCGCAAGTCGTCGGCGCATCGAGCATCTCGATC
TTCCCAGTCCTGTATCTGCAGGCAGGCATCGCGGGCGCGATCCTGCTGCT
CGGCGCGATCGCACTGTACTACTTTGTCGGCGCAAGCCGCAAGTCTGTCG
GCTTCCTTATCGCGACTGATGGGGAGATGAAAAAGGTCAACTGGACTTCC
TATCGCGAAGTGCGCGGCTCGACGATTGTCGTCATTACCGCGACTTTCTT
GATCGCTGGATTCCTCTTTGGTGTAGACACGATCTTCGCGAAAGTGTTCT
CTGCGATCGGCGTCCTTCAGAAATAA
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