mRNA_M-pyrifera_M_contig8991.20840.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig8991.20840.1
Unique NamemRNA_M-pyrifera_M_contig8991.20840.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Match: D8LN69_ECTSI (Putative gamma-glutamylcyclotransferase n=2 Tax=Ectocarpus TaxID=2879 RepID=D8LN69_ECTSI)

HSP 1 Score: 162 bits (411), Expect = 2.350e-48
Identity = 80/109 (73.39%), Postives = 95/109 (87.16%), Query Frame = 1 
Query:  109 AAGGGKGTPLFVYGTLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGLFMEGLDARERLIFDLFEDDDYHKVDVEVKIDGLEEAT-TATAYVW 432
            A G  + TPLFVYGTLMN+KVLFALL+RVP T+KA LSGYHR++I+DHVFPAIRP+EG SV GL M GLDARE+LIFDLFED+DYHKVDV+VK++  ++AT TAT YVW
Sbjct:   20 ADGPREDTPLFVYGTLMNEKVLFALLERVPSTRKASLSGYHRFRIKDHVFPAIRPREGGSVEGLLMTGLDAREKLIFDLFEDEDYHKVDVQVKVEACKDATQTATCYVW 128
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BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Match: A0A836CNX4_9STRA (Putative gamma-glutamylcyclotransferase n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CNX4_9STRA)

HSP 1 Score: 97.4 bits (241), Expect = 1.110e-22
Identity = 53/100 (53.00%), Postives = 69/100 (69.00%), Query Frame = 1 
Query:  136 LFVYGTLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGLFMEGLDARERLIFDLFEDDDYHKVDVEVKI-DGLEEATTATAYVW 432
            +FVYGTLM  +V+  LL  +P ++KA L G+HRY+I+D V+PAIRPKE  SV+G+  +  +  ER IFDLFED DY K  V V + DG   A T +AYVW
Sbjct:   13 VFVYGTLMESRVVTTLLGHMPSSEKAVLPGFHRYRIKDRVYPAIRPKEDGSVSGIVFDVTEREER-IFDLFEDSDYDKETVTVTLEDG--SAVTCSAYVW 109
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BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Match: UPI000CCCF1F1 (AIG2-like protein D isoform X3 n=2 Tax=Populus trichocarpa TaxID=3694 RepID=UPI000CCCF1F1)

HSP 1 Score: 89.0 bits (219), Expect = 1.300e-19
Identity = 47/108 (43.52%), Postives = 66/108 (61.11%), Query Frame = 1 
Query:  109 AAGGGKGTPLFVYGTLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGLFMEGLDARERLIFDLFEDDDYHKVDVEVKIDGLEEATTATAYVW 432
            AAGG +   +FVYG+L+ D V+ ALL R+P +  A L+GYHR+ I+  V+PAI P E   V+G  + G+   E  I D +ED +Y +V V+V +      T A AYVW
Sbjct:    6 AAGGNQVHNVFVYGSLLADDVVRALLSRIPQSSPAILNGYHRFSIKGRVYPAILPVENKKVSGKVLHGITDPELYILDEYEDVEYERVTVDVSLMDNSNKTQALAYVW 113
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BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Match: U5GCL0_POPTR (Putative gamma-glutamylcyclotransferase n=2 Tax=Populus trichocarpa TaxID=3694 RepID=U5GCL0_POPTR)

HSP 1 Score: 89.0 bits (219), Expect = 1.730e-19
Identity = 47/108 (43.52%), Postives = 66/108 (61.11%), Query Frame = 1 
Query:  109 AAGGGKGTPLFVYGTLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGLFMEGLDARERLIFDLFEDDDYHKVDVEVKIDGLEEATTATAYVW 432
            AAGG +   +FVYG+L+ D V+ ALL R+P +  A L+GYHR+ I+  V+PAI P E   V+G  + G+   E  I D +ED +Y +V V+V +      T A AYVW
Sbjct:    6 AAGGNQVHNVFVYGSLLADDVVRALLSRIPQSSPAILNGYHRFSIKGRVYPAILPVENKKVSGKVLHGITDPELYILDEYEDVEYERVTVDVSLMDNSNKTQALAYVW 113
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BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Match: UPI000D188CA7 (AIG2-like protein D isoform X1 n=1 Tax=Populus trichocarpa TaxID=3694 RepID=UPI000D188CA7)

HSP 1 Score: 89.0 bits (219), Expect = 3.870e-19
Identity = 47/108 (43.52%), Postives = 66/108 (61.11%), Query Frame = 1 
Query:  109 AAGGGKGTPLFVYGTLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGLFMEGLDARERLIFDLFEDDDYHKVDVEVKIDGLEEATTATAYVW 432
            AAGG +   +FVYG+L+ D V+ ALL R+P +  A L+GYHR+ I+  V+PAI P E   V+G  + G+   E  I D +ED +Y +V V+V +      T A AYVW
Sbjct:    6 AAGGNQVHNVFVYGSLLADDVVRALLSRIPQSSPAILNGYHRFSIKGRVYPAILPVENKKVSGKVLHGITDPELYILDEYEDVEYERVTVDVSLMDNSNKTQALAYVW 113
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BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Match: A0A1E5W809_9POAL (Putative gamma-glutamylcyclotransferase n=2 Tax=Paniceae TaxID=147428 RepID=A0A1E5W809_9POAL)

HSP 1 Score: 87.4 bits (215), Expect = 8.250e-19
Identity = 42/99 (42.42%), Postives = 60/99 (60.61%), Query Frame = 1 
Query:  136 LFVYGTLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGLFMEGLDARERLIFDLFEDDDYHKVDVEVKIDGLEEATTATAYVW 432
            +FVYG+LM D+V+ A+L RVPP   A L  YHR+ ++  ++PAI P E   VAG+ + G+   E L+ D FED +Y +  VE+ +    E   A  YVW
Sbjct:   19 VFVYGSLMADEVVRAILKRVPPAAPAILPNYHRFNVKGRIYPAILPVESKKVAGMVVMGITDEELLVLDAFEDVEYTRTRVEISLTDSSEKMLADTYVW 117
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BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Match: UPI001F5C8B64 (AIG2-like protein D n=1 Tax=Lolium rigidum TaxID=89674 RepID=UPI001F5C8B64)

HSP 1 Score: 86.7 bits (213), Expect = 1.560e-18
Identity = 44/108 (40.74%), Postives = 65/108 (60.19%), Query Frame = 1 
Query:  109 AAGGGKGTPLFVYGTLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGLFMEGLDARERLIFDLFEDDDYHKVDVEVKIDGLEEATTATAYVW 432
            AAGG     +FVYG+LM D+V+ A+L RVP +  A L+ YHR+ ++D ++PAI P E   VAG  + G+   E ++ D FED +Y +  V++ +    E   A  YVW
Sbjct:   10 AAGGAHS--VFVYGSLMADEVVSAILHRVPASSPALLNDYHRFSVKDRIYPAILPVESKKVAGRVITGISDAELIVLDAFEDYEYVRRTVQISLTDTSETMLADTYVW 115
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BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Match: UPI0009E3A29B (AIG2-like protein D n=1 Tax=Phalaenopsis equestris TaxID=78828 RepID=UPI0009E3A29B)

HSP 1 Score: 86.3 bits (212), Expect = 1.710e-18
Identity = 43/99 (43.43%), Postives = 61/99 (61.62%), Query Frame = 1 
Query:  136 LFVYGTLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGLFMEGLDARERLIFDLFEDDDYHKVDVEVKIDGLEEATTATAYVW 432
            +FVYG+L+ D+V+  LL RVPP   A L+GYHRY ++  V+PAI P E   V G  + G+   E  + D FED++Y +  VEV ++G     +A  YVW
Sbjct:   18 VFVYGSLLADEVVSKLLSRVPPCTPAILNGYHRYNVKGRVYPAILPAERKKVIGRVIMGVTKAELCVLDTFEDEEYKRSTVEVFLNGDSGKLSAETYVW 116
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BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Match: I3SSR9_MEDTR (Putative gamma-glutamylcyclotransferase n=2 Tax=Medicago truncatula TaxID=3880 RepID=I3SSR9_MEDTR)

HSP 1 Score: 86.7 bits (213), Expect = 3.730e-18
Identity = 47/99 (47.47%), Postives = 61/99 (61.62%), Query Frame = 1 
Query:  136 LFVYGTLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGLFMEGLDARERLIFDLFEDDDYHKVDVEVKIDGLEEATTATAYVW 432
            +FVYG+L+ D+V+ ALL RVPP+  A LS YHR++I+D V+PAI P     V G  + G+   E  I D FED +Y K DVEV +    E     AYVW
Sbjct:   28 VFVYGSLLADEVVCALLKRVPPSSPATLSDYHRFKIKDRVYPAILPVHTKKVTGRVLLGISGVELDILDEFEDVEYTKTDVEVFLMDNSENLRVYAYVW 126
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BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Match: A0A4U5QBH5_POPAL (Putative gamma-glutamylcyclotransferase n=2 Tax=Populus alba TaxID=43335 RepID=A0A4U5QBH5_POPAL)

HSP 1 Score: 85.1 bits (209), Expect = 4.350e-18
Identity = 46/107 (42.99%), Postives = 64/107 (59.81%), Query Frame = 1 
Query:  112 AGGGKGTPLFVYGTLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGLFMEGLDARERLIFDLFEDDDYHKVDVEVKIDGLEEATTATAYVW 432
            A G +   +FVYG+L+ D V+ ALL R+P +  A L+GYHR+ I+  V+PAI P E   V+G  + G+   E  I D FED +Y +V V+V      + T A AYVW
Sbjct:   24 AAGNQVHNVFVYGSLLADDVVRALLSRIPQSSPAILNGYHRFSIKGRVYPAILPAENKKVSGKVLHGITDPELYILDEFEDVEYERVTVDVFFMDNSKKTQALAYVW 130
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig8991.20840.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LN69_ECTSI2.350e-4873.39Putative gamma-glutamylcyclotransferase n=2 Tax=Ec... [more]
A0A836CNX4_9STRA1.110e-2253.00Putative gamma-glutamylcyclotransferase n=1 Tax=Tr... [more]
UPI000CCCF1F11.300e-1943.52AIG2-like protein D isoform X3 n=2 Tax=Populus tri... [more]
U5GCL0_POPTR1.730e-1943.52Putative gamma-glutamylcyclotransferase n=2 Tax=Po... [more]
UPI000D188CA73.870e-1943.52AIG2-like protein D isoform X1 n=1 Tax=Populus tri... [more]
A0A1E5W809_9POAL8.250e-1942.42Putative gamma-glutamylcyclotransferase n=2 Tax=Pa... [more]
UPI001F5C8B641.560e-1840.74AIG2-like protein D n=1 Tax=Lolium rigidum TaxID=8... [more]
UPI0009E3A29B1.710e-1843.43AIG2-like protein D n=1 Tax=Phalaenopsis equestris... [more]
I3SSR9_MEDTR3.730e-1847.47Putative gamma-glutamylcyclotransferase n=2 Tax=Me... [more]
A0A4U5QBH5_POPAL4.350e-1842.99Putative gamma-glutamylcyclotransferase n=2 Tax=Po... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig8991contigM-pyrifera_M_contig8991:212..2414 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score91.3
Seed ortholog evalue4.1e-16
Seed eggNOG ortholog3694.POPTR_0006s29320.1
Hectar predicted targeting categoryother localisation
EggNOG free text desc.AIG2-like protein
EggNOG OGs29XB7@1,2RXRF@2759,37TXK@33090,3GIAE@35493,4JPPB@91835
Ec32 ortholog descriptionButirosin biosynthesis, BtrG-like
Ec32 orthologEc-05_003730.1
COG Functional cat.S
Best tax levelfabids
Best eggNOG OGNA|NA|NA
Exons3
Model size435
Cds size435
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig8991.20840.1prot_M-pyrifera_M_contig8991.20840.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig8991 212..2414 -


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816809.804762-CDS-M-pyrifera_M_contig8991:211..3161622816809.804762-CDS-M-pyrifera_M_contig8991:211..316Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8991 212..316 -
1692278191.5622156-CDS-M-pyrifera_M_contig8991:211..3161692278191.5622156-CDS-M-pyrifera_M_contig8991:211..316Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8991 212..316 -
1622816809.8180585-CDS-M-pyrifera_M_contig8991:937..10991622816809.8180585-CDS-M-pyrifera_M_contig8991:937..1099Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8991 938..1099 -
1692278191.5762658-CDS-M-pyrifera_M_contig8991:937..10991692278191.5762658-CDS-M-pyrifera_M_contig8991:937..1099Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8991 938..1099 -
1622816809.829068-CDS-M-pyrifera_M_contig8991:2246..24141622816809.829068-CDS-M-pyrifera_M_contig8991:2246..2414Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8991 2247..2414 -
1692278191.5944996-CDS-M-pyrifera_M_contig8991:2246..24141692278191.5944996-CDS-M-pyrifera_M_contig8991:2246..2414Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8991 2247..2414 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig8991.20840.1

>prot_M-pyrifera_M_contig8991.20840.1 ID=prot_M-pyrifera_M_contig8991.20840.1|Name=mRNA_M-pyrifera_M_contig8991.20840.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=145bp
MEGEERGAATAAAPAAAVAGATAAAAAAAEVGVGGDAAGGGKGTPLFVYG
TLMNDKVLFALLDRVPPTQKARLSGYHRYQIRDHVFPAIRPKEGDSVAGL
FMEGLDARERLIFDLFEDDDYHKVDVEVKIDGLEEATTATAYVW*
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mRNA from alignment at M-pyrifera_M_contig8991:212..2414-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig8991.20840.1 ID=mRNA_M-pyrifera_M_contig8991.20840.1|Name=mRNA_M-pyrifera_M_contig8991.20840.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=2203bp|location=Sequence derived from alignment at M-pyrifera_M_contig8991:212..2414- (Macrocystis pyrifera P11B4 male)
ATGGAGGGGGAAGAACGCGGCGCAGCTACTGCTGCTGCTCCTGCTGCTGC
TGTTGCCGGTGCTACTGCTGCTGCTGCTGCTGCTGCTGAGGTTGGTGTCG
GGGGTGATGCCGCGGGCGGTGGAAAGGGCACGCCTCTGTTCGTGTACGGG
ACTCTCATGAACGACAAGGTGAAAGGTGATGTTTGTCTGTGGTGGTGGGG
GTTGTTGGTGTCGTGATGATGGTGGATGTCGAGGTGGCTTTGGCAGCGTT
GGTGTAGGTGGTGGTGATAATGTTGGTGTCGGGTGTCGTTGGTTGTGTCA
GCAGTGCTGGGGTTTTGTAGTGATGCTGGGATTTGTTGTGGCAGTGGTGG
TGGTGGTGGTTTTGACGTCGGTCGTGTTGGAATCGGTGATGGTATTGGTG
GTGTTGGTGCTAGTGGTGGTAGTGGCGGTGTTGATGGTAGTACGCAGTGC
TTGTATTTTTCGTGTCGGGGATATTGGTCGTGATGCTGGTTTTGTACGGG
ATTGCATACCGATGCGTCGAGCTCCCCTGCGTCGTTTTTTTTCTTTTGGC
TTCCTGAGCTCGCGTGAGAACCCAGAGTGTGTCTACTGTACGCACGGTAA
GCCTCGTGGAGCAAAACCCCGCGCATCTGACCATCACATATCACGCCACG
CTGTCCGGAAAGGTAAGAACCATTTGGTTGTTGATATGAATACCCAAACG
ATCTTTGCGATTCCGGACAGTGCGGTGCTGCATTAAGATACCAACGCCTC
GAACAAATAATGGCACCCAGCTGTTGGTAGTGGTATAAAGCACTTTAGTT
TCCGCGGTACCTGGTATTACTTTACTGTAGACAATGTCTGCCGTTGGAGT
GAGGTCTGTGTAAGGCTTGACCCAGCTTTGGACGAAATGGTTGAAATACT
TAGGTTTGTTCACGGATCGGGATAAGCCTGGCGGGTCAGGTCGGTTCGGG
TCGGGTAAGGGTGACTCCACCCGACCCGTGAAAGTGTGAAAACCTCCTGA
TCCGCCCCGATCCGCCCCTACCCGACCCGTGAGATCTCAAAACGGTTCTG
ACCCCGACCCGCGGGTCGGGCCATGACCTGTCAAATGCCCTGGAAATATG
AATGACTTAAACACTACCCCTGTCGCTAACCCCGACCTCCCGGGAAAATT
CTGGAATTGTGGACTCTTTATCGACCATTGCGTCCTACAGCAGTAGCTGA
TGGGCCAAGTCTTCAGCTCTATGTACTGGCTGAATTCGGACAGCTTTCTG
CAATTGAAGTCGCTGGCAGATATTCTGAACAACAATTTCCCTCCTAACCG
ACACCGCCGATGCAGGTTTTGTTCGCCCTGCTGGACAGAGTCCCACCCAC
CCAGAAGGCGCGACTTTCGGGGTACCACCGCTATCAGATTAGGGACCACG
TTTTCCCGGCCATCCGACCGAAGGAGGGAGATTCCGTGGCAGGGTTGTTT
ATGGAGGGGTTGGACGCTAGAGAGAGGGTGAGGGTGGAGTATATTCCAGG
CGGTTTGTGGGGTTTTTGAGGTGCTCGTACGTACGGCGGCGTTATGGATT
CGATAGTTGGTTTGTGTTGGTAGGTCGATCCGGTGGTTGATTGAGTGGTC
ATCGAATTTTCGGTTGATTGATGGACTGATTGATCACGGAATGGTTACGG
ATTTGGTGTAGTGATTTCTTGATCGGTTGTTAGTTTGTTGAATTTGATTG
CTCGACTGGTCGATTGGTTAGGTGATTGATATATTTTGATTGGTCACTTG
ATTTGCCCCAGTTTCAGTTTTGCCTCTCGCTTGCTTAGTTGCAGTAATTT
ATAGTTGCTTGATGGATTGATCGATGGGTTGGCTGATTGGTTCGCTTGAT
TGGCCCGCCTCCGCTGACGCCTCTCACCACATACGTCAGGAGACACGTCT
AGCAAGGGAAAACGTGCGCTGCCAAGGGTATAGGGCCGAGTGTTCCATAC
TAAATCCAAGTAGCTTGATGTCAGTCAAGGCAAATCAAGGCGAAATTGCT
GTTTTCTGGGGGGTGAAAGGGCTGTCTCCTGACATATTTGCCTCTCACAC
GCCCCCCGCCTGTCTCGGTCTTTGCCGCGACTTTTTGTGGCTGTTCAGCT
AATTTTTGACCTGTTTGAGGACGATGACTACCACAAGGTGGATGTCGAGG
TGAAGATCGATGGCCTCGAGGAAGCGACGACGGCAACGGCGTACGTGTGG
TGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig8991:212..2414-

>mRNA_M-pyrifera_M_contig8991.20840.1 ID=mRNA_M-pyrifera_M_contig8991.20840.1|Name=mRNA_M-pyrifera_M_contig8991.20840.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=870bp|location=Sequence derived from alignment at M-pyrifera_M_contig8991:212..2414- (Macrocystis pyrifera P11B4 male)
ATGGAGGGGGAAGAACGCGGCGCAGCTACTGCTGCTGCTCCTGCTGCTGC
TGTTGCCGGTGCTACTGCTGCTGCTGCTGCTGCTGCTGAGGTTGGTGTCG
GGGGTGATGCCGCGGGCGGTGGAAAGGGCACGCCTCTGTTCGTGTACGGG
ACTCTCATGAACGACAAGATGGAGGGGGAAGAACGCGGCGCAGCTACTGC
TGCTGCTCCTGCTGCTGCTGTTGCCGGTGCTACTGCTGCTGCTGCTGCTG
CTGCTGAGGTTGGTGTCGGGGGTGATGCCGCGGGCGGTGGAAAGGGCACG
CCTCTGTTCGTGTACGGGACTCTCATGAACGACAAGGTTTTGTTCGCCCT
GCTGGACAGAGTCCCACCCACCCAGAAGGCGCGACTTTCGGGGTACCACC
GCTATCAGATTAGGGACCACGTTTTCCCGGCCATCCGACCGAAGGAGGGA
GATTCCGTGGCAGGGTTGTTTATGGAGGGGTTGGACGCTAGAGAGAGGGT
TTTGTTCGCCCTGCTGGACAGAGTCCCACCCACCCAGAAGGCGCGACTTT
CGGGGTACCACCGCTATCAGATTAGGGACCACGTTTTCCCGGCCATCCGA
CCGAAGGAGGGAGATTCCGTGGCAGGGTTGTTTATGGAGGGGTTGGACGC
TAGAGAGAGGCTAATTTTTGACCTGTTTGAGGACGATGACTACCACAAGG
TGGATGTCGAGGTGAAGATCGATGGCCTCGAGGAAGCGACGACGGCAACG
GCGTACGTGTGGTGACTAATTTTTGACCTGTTTGAGGACGATGACTACCA
CAAGGTGGATGTCGAGGTGAAGATCGATGGCCTCGAGGAAGCGACGACGG
CAACGGCGTACGTGTGGTGA
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