mRNA_M-pyrifera_M_contig89767.20807.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig89767.20807.1
Unique NamemRNA_M-pyrifera_M_contig89767.20807.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Match: A0A0L0S8W1_ALLM3 (Valine--tRNA ligase n=1 Tax=Allomyces macrogynus (strain ATCC 38327) TaxID=578462 RepID=A0A0L0S8W1_ALLM3)

HSP 1 Score: 221 bits (563), Expect = 4.630e-68
Identity = 96/180 (53.33%), Postives = 131/180 (72.78%), Query Frame = 1
Query:    4 PPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQSVVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAWEKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEVDTLEIHKKSTLTLPNGEKVQVGTMDYFRYEI 543
            PPPNVTG LH GHA+  A+QDA+ R+H   GK+V W+PG DHAGIATQ+VVE++L   EG+TRH+LGR+ FL RVW W+ A+G  I+ QL+ +GA+ +W   YFTLD  RSR V +AF+ L  + L++R T++VHWCP L+TA++D+EV+ L I   + +  P+G   QVG M YFRY +
Sbjct:   55 PPPNVTGRLHCGHALTTAIQDAIARYHHMHGKSVRWIPGTDHAGIATQTVVEKQLAKTEGKTRHELGRDAFLSRVWSWKDAHGDHIHHQLRALGASFSWSDAYFTLDTPRSRIVTDAFLELSRRGLIYRATRLVHWCPALETAISDMEVEYLTIDGPTAVPTPSGHSAQVGEMHYFRYPV 234          
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Match: G4ZE43_PHYSP (Valine--tRNA ligase n=6 Tax=Phytophthora TaxID=4783 RepID=G4ZE43_PHYSP)

HSP 1 Score: 231 bits (590), Expect = 9.410e-68
Identity = 110/182 (60.44%), Postives = 140/182 (76.92%), Query Frame = 1
Query:    1 LPPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQSVVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAWEKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEVDTLEIHKKSTLTLPNGEK-VQVGTMDYFRYEI 543
            LPPPNVTGALHLGHA+ V +QDA+ RWHR RG  V W+PGLDHAGIATQSVVER+L  E+G +RHDLGRE F+ +VW W   YGGRI  Q+  +GA +  ++ YFTLDE+RSRAVV+AFV+LHEK L++R  +MV+WCP LQTA++D+EVD  ++ K +   LP  EK V+ G M  F+Y+I
Sbjct:    3 LPPPNVTGALHLGHALTVTIQDAIARWHRMRGFDVRWLPGLDHAGIATQSVVERKLLKEKGLSRHDLGREAFVQQVWAWNEQYGGRIMSQIDHLGAIVKKDQPYFTLDEQRSRAVVDAFVKLHEKGLVYRRRRMVNWCPTLQTAISDIEVDLEQLEKPTKKMLPGREKAVEFGVMHRFKYQI 184          
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Match: A0A7J7IPL4_9RHOD (Valine--tRNA ligase n=1 Tax=Cyanidiococcus yangmingshanensis TaxID=2690220 RepID=A0A7J7IPL4_9RHOD)

HSP 1 Score: 221 bits (564), Expect = 4.210e-67
Identity = 102/183 (55.74%), Postives = 139/183 (75.96%), Query Frame = 1
Query:    1 LPPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQSVVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAWEKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEVDTLEIHKKSTLTLPNGE-KVQVGTMDYFRYEIK 546
            +PPPNVTG LHLGH +M A+QD L RWHR +G   LWVPG DHAGIATQ+VVER+L  E   TRHDLGRE F+  VW ++  YGGRI +QL+++GA++ W +E FT++EK SRAVVEAFVRLHE+ L++RGT++V+WC  L+TAL+D+EV+ ++I  +    +P  E +V+VG +  F Y ++
Sbjct:  164 MPPPNVTGTLHLGHTLMCAIQDCLTRWHRMKGHVTLWVPGTDHAGIATQTVVERKLMKERKLTRHDLGREDFVQYVWSYKREYGGRICDQLRRLGASVDWSREQFTMNEKLSRAVVEAFVRLHERGLIYRGTRLVNWCCRLRTALSDIEVEYVDIPGRELRRVPGHEAEVEVGVLTQFAYPLE 346          
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Match: W2Q1L0_PHYPN (Valine--tRNA ligase n=1 Tax=Phytophthora parasitica (strain INRA-310) TaxID=761204 RepID=W2Q1L0_PHYPN)

HSP 1 Score: 221 bits (564), Expect = 4.310e-67
Identity = 102/182 (56.04%), Postives = 139/182 (76.37%), Query Frame = 1
Query:    1 LPPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQSVVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAWEKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEVDTLEIHKKSTLTLPNGEK-VQVGTMDYFRYEI 543
            LPPPNVTGALH+GHA+ + +QDA+ RWHR RG  V W+PGLDHAGIATQSVVER+L  E+G +R+DLGRE F+ +VW+W   YGGRI  Q+  +GA +  ++ YFTLD++RS AV+ AFV L+EK L++R  +MV+WCP LQTA++D+EVD+ ++ K +   LP  +K V+ G M  F+Y+I
Sbjct:  104 LPPPNVTGALHIGHALTITIQDAIARWHRMRGFDVRWLPGLDHAGIATQSVVERKLHKEQGLSRYDLGREAFVDQVWQWNEQYGGRIMNQIDHLGAIVKKDQPYFTLDKQRSEAVINAFVTLYEKGLVYRKRRMVNWCPTLQTAISDIEVDSEQLEKATKKMLPGRDKPVEFGVMHRFKYQI 285          
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Match: A0A0P1AAC4_PLAHL (Valine--tRNA ligase n=1 Tax=Plasmopara halstedii TaxID=4781 RepID=A0A0P1AAC4_PLAHL)

HSP 1 Score: 230 bits (587), Expect = 6.300e-67
Identity = 105/182 (57.69%), Postives = 140/182 (76.92%), Query Frame = 1
Query:    1 LPPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQSVVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAWEKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEVDTLEIHKKSTLTLPNGEKV-QVGTMDYFRYEI 543
            LPPPN+TGALH+GHA+ + +QDA+ RWHR RG  V W+PGLDHAGIATQSVVER+L  E+G +RH++GRE F+ RVW+W   YGG+I  Q+ ++GA +  ++ YFTLD +RS+AVV AFV+LHEK LL+RG +MV+WCP LQTA++D+EVD   + K +   LP G KV + G M  F+Y+I
Sbjct:   98 LPPPNITGALHIGHALTITIQDAIARWHRMRGFDVRWLPGLDHAGIATQSVVERKLMKEQGLSRHEIGREAFVDRVWQWNEQYGGKIMNQIDRLGAIVKKDQPYFTLDTERSKAVVTAFVKLHEKGLLYRGRRMVNWCPMLQTAISDIEVDLEPLDKATKKVLPGGHKVVEFGVMYRFKYQI 279          
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Match: A0A0W8CTI7_PHYNI (Valine--tRNA ligase n=1 Tax=Phytophthora nicotianae TaxID=4790 RepID=A0A0W8CTI7_PHYNI)

HSP 1 Score: 221 bits (564), Expect = 7.490e-67
Identity = 102/182 (56.04%), Postives = 139/182 (76.37%), Query Frame = 1
Query:    1 LPPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQSVVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAWEKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEVDTLEIHKKSTLTLPNGEK-VQVGTMDYFRYEI 543
            LPPPNVTGALH+GHA+ + +QDA+ RWHR RG  V W+PGLDHAGIATQSVVER+L  E+G +R+DLGRE F+ +VW+W   YGGRI  Q+  +GA +  ++ YFTLD++RS AV+ AFV L+EK L++R  +MV+WCP LQTA++D+EVD+ ++ K +   LP  +K V+ G M  F+Y+I
Sbjct:  104 LPPPNVTGALHIGHALTITIQDAIARWHRMRGFDVRWLPGLDHAGIATQSVVERKLHKEQGLSRYDLGREAFVDQVWQWNEQYGGRIMNQIDHLGAIVKKDQPYFTLDKQRSEAVINAFVTLYEKGLVYRKRRMVNWCPTLQTAISDIEVDSEQLEKATKKMLPGRDKPVEFGVMHRFKYQI 285          
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Match: A0A6A4ZPF2_9STRA (Valine--tRNA ligase (Fragment) n=1 Tax=Aphanomyces astaci TaxID=112090 RepID=A0A6A4ZPF2_9STRA)

HSP 1 Score: 217 bits (552), Expect = 8.680e-67
Identity = 101/182 (55.49%), Postives = 134/182 (73.63%), Query Frame = 1
Query:    1 LPPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQSVVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAWEKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEVDTLEIHKKSTLTLP-NGEKVQVGTMDYFRYEI 543
            LPPPN+TGALH+GHA+ V +QDAL R+HR  G  VLW+PGLDHAGIATQSVVE++LW E   TRHD+GR  FL  V  W   YG RI  QL+++GA L  +  +FTLD+ RS+AVV+AF RLH K L++R  +MV+WCP LQTA++D+EVD + + K++ L LP +   V+ G M  F+Y++
Sbjct:   75 LPPPNITGALHIGHALTVTIQDALARYHRMCGHEVLWIPGLDHAGIATQSVVEKQLWKEHKLTRHDVGRSAFLDHVHTWNATYGSRILNQLERLGALLNKDHTFFTLDDTRSKAVVDAFCRLHAKGLVYRHRRMVNWCPTLQTAISDIEVDAVPLTKRTMLPLPGHATPVEFGVMHRFKYKV 256          
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Match: A0A225VG97_9STRA (Valine--tRNA ligase (Fragment) n=1 Tax=Phytophthora megakarya TaxID=4795 RepID=A0A225VG97_9STRA)

HSP 1 Score: 227 bits (578), Expect = 1.530e-66
Identity = 105/182 (57.69%), Postives = 139/182 (76.37%), Query Frame = 1
Query:    1 LPPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQSVVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAWEKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEVDTLEIHKKSTLTLPNGEK-VQVGTMDYFRYEI 543
            LPPPNVTGALH+GHA+ + +QDA+ RWHR RG  V W+PGLDHAGIATQSVVER+L  E G +R+DLGRE F+  VW+W   YGG+I  Q+  +GA +  ++ YFTLDEKRS AVVEAFV+L+EK L++R  +MV+WCP LQTA++D+EVD+ ++ K +   LP  EK V+ G M  F+Y++
Sbjct:  105 LPPPNVTGALHIGHALTITIQDAIARWHRMRGFDVRWLPGLDHAGIATQSVVERKLHKERGLSRYDLGREAFVDEVWQWNEQYGGKIMSQIDHLGAIVKKDQPYFTLDEKRSEAVVEAFVKLYEKGLIYRRRRMVNWCPTLQTAISDIEVDSEQLEKATKKMLPGREKAVEFGVMHRFKYQV 286          
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Match: A0A2D4C7J2_PYTIN (Valine--tRNA ligase n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4C7J2_PYTIN)

HSP 1 Score: 227 bits (579), Expect = 2.010e-66
Identity = 107/182 (58.79%), Postives = 138/182 (75.82%), Query Frame = 1
Query:    1 LPPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQSVVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAWEKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEVDTLEIHKKSTLTLPNGEK-VQVGTMDYFRYEI 543
            LPPPNVTGALH+GHA+ + +Q AL RWH  RG  V W+PGLDHAGIATQSVVER+L  +EGRTRHDLGRE F+ RVW+W   YGGRI  Q+ ++GA +  E  +FTLD+KRS AVVEAFV+LHE+ L++R  +MV+WCP L+TA++D+EVD+  +   +  TLP   K V+ G M  F+Y+I
Sbjct:   91 LPPPNVTGALHIGHALTITIQXALARWHHMRGFDVQWIPGLDHAGIATQSVVERKLMKDEGRTRHDLGREAFVERVWQWNEQYGGRILHQIDQLGAIVDKEASFFTLDDKRSAAVVEAFVQLHERGLVYRKRRMVNWCPTLKTAISDIEVDSESLTGSTMKTLPGRXKPVEFGVMHRFKYQI 272          
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Match: A0A0G4HLJ6_9ALVE (Valine--tRNA ligase (Fragment) n=1 Tax=Chromera velia CCMP2878 TaxID=1169474 RepID=A0A0G4HLJ6_9ALVE)

HSP 1 Score: 219 bits (557), Expect = 2.900e-66
Identity = 98/183 (53.55%), Postives = 138/183 (75.41%), Query Frame = 1
Query:    1 LPPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQSVVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAWEKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEVDTLEIHKKSTLTLPNGEK-VQVGTMDYFRYEIK 546
            +PPPNVTG LH+GH +  A++D L RWHR +G   LWVPG DHAGIATQ+VVER++   EG+TRHD GRE F+ +VWEW+   GG I  Q +++ A++ W +E FTLD+K S+AV EAFVR+ +  L++RGT++V WCPHL+TAL+D+EVDT ++   S +++P  +K V+VG + +F Y+IK
Sbjct:  166 IPPPNVTGNLHIGHTLTTAIEDTLTRWHRMKGDVSLWVPGQDHAGIATQTVVERQIQKLEGKTRHDYGREAFVNKVWEWKKQKGGNICMQTRRLAASVDWSREAFTLDDKLSKAVKEAFVRMFDAGLIYRGTRLVSWCPHLRTALSDIEVDTDDVEPFSKVSIPGFDKQVEVGVLVHFLYKIK 348          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig89767.20807.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A0L0S8W1_ALLM34.630e-6853.33Valine--tRNA ligase n=1 Tax=Allomyces macrogynus (... [more]
G4ZE43_PHYSP9.410e-6860.44Valine--tRNA ligase n=6 Tax=Phytophthora TaxID=478... [more]
A0A7J7IPL4_9RHOD4.210e-6755.74Valine--tRNA ligase n=1 Tax=Cyanidiococcus yangmin... [more]
W2Q1L0_PHYPN4.310e-6756.04Valine--tRNA ligase n=1 Tax=Phytophthora parasitic... [more]
A0A0P1AAC4_PLAHL6.300e-6757.69Valine--tRNA ligase n=1 Tax=Plasmopara halstedii T... [more]
A0A0W8CTI7_PHYNI7.490e-6756.04Valine--tRNA ligase n=1 Tax=Phytophthora nicotiana... [more]
A0A6A4ZPF2_9STRA8.680e-6755.49Valine--tRNA ligase (Fragment) n=1 Tax=Aphanomyces... [more]
A0A225VG97_9STRA1.530e-6657.69Valine--tRNA ligase (Fragment) n=1 Tax=Phytophthor... [more]
A0A2D4C7J2_PYTIN2.010e-6658.79Valine--tRNA ligase n=1 Tax=Pythium insidiosum Tax... [more]
A0A0G4HLJ6_9ALVE2.900e-6653.55Valine--tRNA ligase (Fragment) n=1 Tax=Chromera ve... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig89767contigM-pyrifera_M_contig89767:2..547 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeApicomplexa
Seed ortholog score225.3
Seed ortholog evalue2.3e-56
Seed eggNOG ortholog5865.XP_001611967.1
KEGG rclassRC00055,RC00523
KEGG koko:K01873
KEGG ReactionR03665
KEGG Pathwayko00970,map00970
KEGG ModuleM00359,M00360
Hectar predicted targeting categoryother localisation
GOsGO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0044424,GO:0044464
EggNOG free text desc.Belongs to the class-I aminoacyl-tRNA synthetase family
EggNOG OGs3KBZ5@422676,3Y9PR@5794,3Z4U8@5863,COG0525@1,KOG0432@2759
EC6.1.1.9
COG Functional cat.J
Best tax levelPiroplasmida
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko01007,ko03016
Exons1
Model size546
Cds size546
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816808.5198617-CDS-M-pyrifera_M_contig89767:1..5471622816808.5198617-CDS-M-pyrifera_M_contig89767:1..547Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89767 2..547 +
1692278190.868809-CDS-M-pyrifera_M_contig89767:1..5471692278190.868809-CDS-M-pyrifera_M_contig89767:1..547Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89767 2..547 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig89767.20807.1prot_M-pyrifera_M_contig89767.20807.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig89767 2..547 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig89767.20807.1

>prot_M-pyrifera_M_contig89767.20807.1 ID=prot_M-pyrifera_M_contig89767.20807.1|Name=mRNA_M-pyrifera_M_contig89767.20807.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=182bp
LPPPNVTGALHLGHAMMVAVQDALVRWHRQRGKAVLWVPGLDHAGIATQS
VVERELWGEEGRTRHDLGREGFLGRVWEWRGAYGGRINEQLQKMGATLAW
EKEYFTLDEKRSRAVVEAFVRLHEKKLLFRGTQMVHWCPHLQTALADLEV
DTLEIHKKSTLTLPNGEKVQVGTMDYFRYEIK
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mRNA from alignment at M-pyrifera_M_contig89767:2..547+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig89767.20807.1 ID=mRNA_M-pyrifera_M_contig89767.20807.1|Name=mRNA_M-pyrifera_M_contig89767.20807.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=546bp|location=Sequence derived from alignment at M-pyrifera_M_contig89767:2..547+ (Macrocystis pyrifera P11B4 male)
CTTCCGCCACCGAATGTCACGGGGGCGCTGCACTTGGGCCACGCGATGAT GGTGGCGGTGCAGGATGCGTTGGTGCGATGGCACAGGCAGCGTGGGAAGG CGGTGTTGTGGGTGCCTGGGTTGGATCACGCGGGGATCGCGACACAGAGT GTGGTGGAGCGGGAACTGTGGGGGGAGGAGGGGAGGACGCGGCATGATCT GGGCAGGGAAGGGTTTCTGGGGCGCGTGTGGGAGTGGAGGGGCGCGTACG GGGGCAGAATCAATGAGCAGCTGCAAAAGATGGGGGCGACGCTGGCCTGG GAAAAAGAGTATTTCACGCTGGACGAAAAGAGGAGCAGGGCTGTGGTGGA GGCGTTTGTGAGACTGCACGAGAAAAAACTCCTCTTCCGAGGCACGCAAA TGGTGCACTGGTGCCCCCACCTCCAAACTGCACTAGCTGACCTCGAAGTG GACACCCTCGAAATCCACAAGAAATCCACTCTCACACTCCCAAACGGAGA AAAAGTGCAAGTTGGAACAATGGACTACTTCCGATACGAAATCAAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig89767:2..547+

>mRNA_M-pyrifera_M_contig89767.20807.1 ID=mRNA_M-pyrifera_M_contig89767.20807.1|Name=mRNA_M-pyrifera_M_contig89767.20807.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1092bp|location=Sequence derived from alignment at M-pyrifera_M_contig89767:2..547+ (Macrocystis pyrifera P11B4 male)
CTTCCGCCACCGAATGTCACGGGGGCGCTGCACTTGGGCCACGCGATGAT
GGTGGCGGTGCAGGATGCGTTGGTGCGATGGCACAGGCAGCGTGGGAAGG
CGGTGTTGTGGGTGCCTGGGTTGGATCACGCGGGGATCGCGACACAGAGT
GTGGTGGAGCGGGAACTGTGGGGGGAGGAGGGGAGGACGCGGCATGATCT
GGGCAGGGAAGGGTTTCTGGGGCGCGTGTGGGAGTGGAGGGGCGCGTACG
GGGGCAGAATCAATGAGCAGCTGCAAAAGATGGGGGCGACGCTGGCCTGG
GAAAAAGAGTATTTCACGCTGGACGAAAAGAGGAGCAGGGCTGTGGTGGA
GGCGTTTGTGAGACTGCACGAGAAAAAACTCCTCTTCCGAGGCACGCAAA
TGGTGCACTGGTGCCCCCACCTCCAAACTGCACTAGCTGACCTCGAAGTG
GACACCCTCGAAATCCACAAGAAATCCACTCTCACACTCCCAAACGGAGA
AAAAGTGCAAGTTGGAACAATGGACTACTTCCGATACGAAATCAAGCTTC
CGCCACCGAATGTCACGGGGGCGCTGCACTTGGGCCACGCGATGATGGTG
GCGGTGCAGGATGCGTTGGTGCGATGGCACAGGCAGCGTGGGAAGGCGGT
GTTGTGGGTGCCTGGGTTGGATCACGCGGGGATCGCGACACAGAGTGTGG
TGGAGCGGGAACTGTGGGGGGAGGAGGGGAGGACGCGGCATGATCTGGGC
AGGGAAGGGTTTCTGGGGCGCGTGTGGGAGTGGAGGGGCGCGTACGGGGG
CAGAATCAATGAGCAGCTGCAAAAGATGGGGGCGACGCTGGCCTGGGAAA
AAGAGTATTTCACGCTGGACGAAAAGAGGAGCAGGGCTGTGGTGGAGGCG
TTTGTGAGACTGCACGAGAAAAAACTCCTCTTCCGAGGCACGCAAATGGT
GCACTGGTGCCCCCACCTCCAAACTGCACTAGCTGACCTCGAAGTGGACA
CCCTCGAAATCCACAAGAAATCCACTCTCACACTCCCAAACGGAGAAAAA
GTGCAAGTTGGAACAATGGACTACTTCCGATACGAAATCAAG
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