mRNA_M-pyrifera_M_contig89711.20799.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig89711.20799.1
Unique NamemRNA_M-pyrifera_M_contig89711.20799.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Match: A0A5A8DGM4_CAFRO (Uncharacterized protein n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DGM4_CAFRO)

HSP 1 Score: 116 bits (290), Expect = 8.330e-27
Identity = 61/180 (33.89%), Postives = 111/180 (61.67%), Query Frame = 1
Query:    1 FGPYSALEVIRARRVFEGLDINGSGDVDMEELKGSSEFRQMYSDERIAVMFDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMTHLRRRLAARVKKET-LRDE-LTDGQRAEIAAIFRAFDRDDDGTITVNEIKQTLKAQGELFADIWADLQLDTIFNRYDKDKSLSMDLTEFTSLM 534
            FGPYS LEVIR R++FE +D + +G + +E+L    ++   +  +R+A +++ ++ + SG ++L ELF ++FP+A+R ++ +M+ +T   + ++ R KKET L D  +T+    EI AIF + D + DG +++ EI++  K + +   +      L+++  RYD D +  +DL EF  LM
Sbjct: 1670 FGPYSTLEVIRIRQLFEAVDADSTGVISLEQLCNHPDWGASHGQDRLANLYEVMEQERSGDVTLSELFTIMFPLANRAMISRMVRVTASHKAVSLRPKKETPLEDSTVTEAHLLEIKAIFNSIDENRDGELSMAEIREAFKEKRKAAKESLGMRALESVIARYDADDNKGLDLEEFAELM 1849          
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Match: A0A5A8CB72_CAFRO (Uncharacterized protein n=5 Tax=Sar TaxID=2698737 RepID=A0A5A8CB72_CAFRO)

HSP 1 Score: 116 bits (290), Expect = 8.470e-27
Identity = 61/180 (33.89%), Postives = 111/180 (61.67%), Query Frame = 1
Query:    1 FGPYSALEVIRARRVFEGLDINGSGDVDMEELKGSSEFRQMYSDERIAVMFDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMTHLRRRLAARVKKET-LRDE-LTDGQRAEIAAIFRAFDRDDDGTITVNEIKQTLKAQGELFADIWADLQLDTIFNRYDKDKSLSMDLTEFTSLM 534
            FGPYS LEVIR R++FE +D + +G + +E+L    ++   +  +R+A +++ ++ + SG ++L ELF ++FP+A+R ++ +M+ +T   + ++ R KKET L D  +T+    EI AIF + D + DG +++ EI++  K + +   +      L+++  RYD D +  +DL EF  LM
Sbjct: 2311 FGPYSTLEVIRIRQLFEAVDADSTGVISLEQLCNHPDWGASHGQDRLANLYEVMEQERSGDVTLSELFTIMFPLANRAMISRMVRVTASHKAVSLRPKKETPLEDSTVTEAHLLEIKAIFNSIDENRDGELSMAEIREAFKEKRKAAKESLGMRALESVIARYDADDNKGLDLEEFAELM 2490          
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Match: A0A421GQD1_9STRA (Uncharacterized protein n=3 Tax=Phytophthora kernoviae TaxID=325452 RepID=A0A421GQD1_9STRA)

HSP 1 Score: 73.9 bits (180), Expect = 3.900e-13
Identity = 56/187 (29.95%), Postives = 99/187 (52.94%), Query Frame = 1
Query:    1 FGPYSALEVIRARRVFEGLDINGSGDVDMEELKGSSE-FRQMYSDERIAVMFDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMTHLRRRLAARVKKETLRDELTDGQRA-EIAAIFRAFDRDDDGTITVNEIKQTLKAQGELF-ADIWADLQLDT-----IFNRYDKDKSLSMDLTEFTSLMQ 537
            FGPY+  +V+   R F  LD +GSG + + EL G +  F   +  + ++ +F +ID D SG I L EL  V+F  A   +L+   E+  L R L A  K  +++ +   G++  E+ A+F+ +D+DD+G I  +E+ + L+     + +D     QL       I + +D D + ++++ EF  L +
Sbjct:    6 FGPYARDDVVNIFRTFRKLDQDGSGSIALAELIGGTGLFSGTHMQDNVSSIFSSIDKDQSGLIDLDELCTVIFGDAPPEVLE---EIKRLCRLLEAVEKTRSVQKKPLSGEQVKELQALFKLYDKDDNGDIDASELFEALRYNDRFYDSDRRGSSQLSMADVMRIISAFDVDANATLNMDEFIELFR 189          
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Match: T0RWW2_SAPDV (Uncharacterized protein n=2 Tax=Saprolegnia TaxID=4769 RepID=T0RWW2_SAPDV)

HSP 1 Score: 74.7 bits (182), Expect = 2.350e-12
Identity = 50/139 (35.97%), Postives = 74/139 (53.24%), Query Frame = 1
Query:    1 FGPYSALEVIRARRVFEGLDINGSGDVDMEELKGSSEFRQMYSDERIAVMFDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMTHLRRRLAARVKKETLRDELTDGQRAEIAAIFRAFDRDDDGTITVNEIKQTLK 417
            FG YS  EV+   R+F  LD + SG+V   EL G   F +      +A +F TIDAD+SG ++L ELF + F  A+   + +M+ +  L R      + ++L  E    Q AE+  IF  FDRD  GT++  E+   L+
Sbjct: 1293 FGIYSTKEVMVIIRLFWALDSDNSGNVTEMELNGCRGFFESMGYTDMATIFQTIDADASGHVTLAELFKICFAYATSAEIHEMVMLERLGRAPTIFTEDKSLTHE----QIAELREIFTVFDRDRSGTVSFAEVLDALQ 1427          
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Match: A0A1V9ZD18_9STRA (Uncharacterized protein n=1 Tax=Thraustotheca clavata TaxID=74557 RepID=A0A1V9ZD18_9STRA)

HSP 1 Score: 70.1 bits (170), Expect = 9.320e-11
Identity = 47/143 (32.87%), Postives = 73/143 (51.05%), Query Frame = 1
Query:    1 FGPYSALEVIRARRVFEGLDINGSGDVDMEELKGSSEFRQMYSDERIAVMFDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMTHLRRRLAARVKKETLRDELTDGQRAEIAAIFRAFDRDDDGTITVNEIKQTLKAQGE 429
            FG Y+  EV+   R+F  LD + SG V  +EL G   + +      +A +F TID D SG++SL EL  + FP AS   +  M+ +  L R  +     + L  E    Q  E+  IF  FD D  GT++ +E+ + L+ + +
Sbjct: 1312 FGIYTTKEVMVIIRLFWMLDTDCSGSVTEDELDGCRNYFESMGYTDMATIFQTIDTDRSGEVSLAELLKICFPYASPTDIHDMLALEKLGRSPSLFADSKILTGE----QLNELREIFNVFDADHSGTVSFDEVLEALQCKDD 1450          
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Match: A0A6A3X208_9STRA (Uncharacterized protein n=3 Tax=Phytophthora fragariae TaxID=53985 RepID=A0A6A3X208_9STRA)

HSP 1 Score: 68.9 bits (167), Expect = 2.420e-10
Identity = 51/219 (23.29%), Postives = 100/219 (45.66%), Query Frame = 1
Query:    1 FGPYSALEVIRARRVFEGLDINGSGDVDMEELKGSSEFRQMYSDERIAVMFDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMTHLRR-----------RLAARVKKETLRD-------ELTDGQRAEIAAIFRAFDRDDDGTITVNEIKQTLKAQGELFADIWADLQ-----------------------LDTIFNRYDKDKSLSMDLTEFTSLM 534
            FG Y+  EV+   R+F  +D++GSG++ ++EL+             +A +F TID++   Q+SL+EL    F  A++  +  M+++  +              + +       R+       +L    R+E+ AIFR FD + DG +++ EI ++L+   +   D+ A +                        ++ I+  +D+DK  ++D  EF ++M
Sbjct: 2360 FGMYTVQEVMSVIRLFWSMDVDGSGNISLDELQRFKSMFDKLGYHNLAAIFQTIDSNXXXQVSLRELLSTCFHYATKEQIDGMLQLAKVGSVRSFLFGLDGAAMGSEKASSPTRNPFANPGGKLLPEHRSELMAIFRVFDHNGDGGVSMQEIMESLRVDDD---DVMAAVMTREHKAGGRSSEPVVSSGLTREDVEQIYREFDRDKDATLDFDEFVAVM 2575          
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Match: A0A6A3IQF4_9STRA (Uncharacterized protein n=2 Tax=Phytophthora TaxID=4783 RepID=A0A6A3IQF4_9STRA)

HSP 1 Score: 66.6 bits (161), Expect = 1.500e-9
Identity = 50/219 (22.83%), Postives = 99/219 (45.21%), Query Frame = 1
Query:    1 FGPYSALEVIRARRVFEGLDINGSGDVDMEELKGSSEFRQMYSDERIAVMFDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMTHLRR-----------RLAARVKKETLRD-------ELTDGQRAEIAAIFRAFDRDDDGTITVNEIKQTLKAQGELFADIWADLQ-----------------------LDTIFNRYDKDKSLSMDLTEFTSLM 534
            FG Y+  EV+   R+F  +D++GSG++ ++EL+             +A +F TID++   Q+SL+EL    F  A++  +  M+++  +              + +       R+       +L    R ++ AIFR FD + DG +++ EI ++L+   +   D+ A +                        ++ I+  +D+DK  ++D  EF ++M
Sbjct: 1771 FGMYTVQEVMSVIRLFWSMDVDGSGNISLDELQRFKSMFDKLGYHNLAAIFQTIDSNXXXQVSLRELLSTCFHYATKEQIDGMLQLAKVGSVRSFLFGLDGAAMGSEKASSPTRNPFANPGGKLLPEHRIKLMAIFRVFDHNGDGGVSMQEIMESLRVDDD---DVMAAVMTREHKAGGRSSEPVVSSGLTREDVEQIYREFDRDKDATLDFDEFVAVM 1986          
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Match: A0A2D4CDJ0_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4CDJ0_PYTIN)

HSP 1 Score: 64.3 bits (155), Expect = 6.710e-9
Identity = 57/210 (27.14%), Postives = 94/210 (44.76%), Query Frame = 1
Query:    1 FGPYSALEVIRARRVFEGLDINGSGDVDMEELKGSSEFRQMYSDERIAVMFDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMT---HLRRRLAARVKKETLRDELTDG---QRAEIAAIFRAFDRDDDGTITVNEIKQTLK--------AQGELFADIWAD---------------LQLDTI---FNRYDKDKSLSMDLTEFTSLM 534
            FG YS  E++   R+F  LD + SG V +EE++    F      + +A +F  +D D +G ++L+EL  + F  A    L  M+ +    ++   LA      + R    D    +R E+  IFR FDR+ DG +++ E+ Q L         A G + +  W++               L  D +   ++ YDK+    +D  EF  LM
Sbjct:  103 FGLYSIREIMAVIRLFWFLDADRSGGVTLEEIRRHEGFFSGLGYDDVATVFGEMDRDGNGVVTLRELLRLCFARAKPHQLDAMVTLAKVGNIEEYLAGHDASPSERVASADAIAQRRLELLEIFRLFDRNGDGKVSMQELLQALHVDEQEQPWAVGAVSSRTWSNAAASAAAVNRARSGSLTKDDVVRFYHEYDKNGDAELDFDEFLKLM 312          
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Match: A0A067BCI8_SAPPC (Uncharacterized protein n=1 Tax=Saprolegnia parasitica (strain CBS 223.65) TaxID=695850 RepID=A0A067BCI8_SAPPC)

HSP 1 Score: 59.3 bits (142), Expect = 4.870e-8
Identity = 46/144 (31.94%), Postives = 70/144 (48.61%), Query Frame = 1
Query:  124 YSDERIAVMFDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMTHLRRRLAARVKKETLRDELTDGQRAEIAAIFRAFDRDDDGTITVNEIKQTLKAQ------GELFADIWADLQLDTIFNRYDKDKSLSMDLTEFTSLMQ 537
            YSD  +A +F TIDAD+SG ++L ELF + F  A+   + +M+ +  L R      + + L    T  Q AE+  IF  FDRD  GT++  E+   L+ +      G   AD      L  ++   D D +  +   EF  L+Q
Sbjct:    3 YSD--MATIFQTIDADASGHVTLAELFKICFAYATSAEIHEMVMLERLGRAPTVFAEDKPL----TPDQIAELREIFTVFDRDRSGTVSFAEVLDALQYRHDDDDAGRTLAD------LAKLYEAADNDGNKELSFDEFVQLLQ 134          
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Match: A0A662WFT7_9STRA (Uncharacterized protein n=1 Tax=Nothophytophthora sp. Chile5 TaxID=2483409 RepID=A0A662WFT7_9STRA)

HSP 1 Score: 61.6 bits (148), Expect = 7.920e-8
Identity = 50/213 (23.47%), Postives = 90/213 (42.25%), Query Frame = 1
Query:    1 FGPYSALEVIRARRVFEGLDINGSGDVDMEELKGSSEFRQMYSDERIAVMFDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMTHLRRRLAARVKKETLRDELTDG---------QRAEIAAIFRAFDRDDDGTITVNEIKQTLK-------------------------AQGELFADIWADLQLDTIFNRYDKDKSLSMDLTEFTSLMQ 537
            FG YS  EV+   R+F  +D++GSG + M EL+  + F +      +A +F               L  + F  A++V L+ M+++  +    +    +    D +  G         QR E+ AIFR FD + DG +++ EI + L+                         A G + A       ++ ++  +D D+  ++D  EF ++MQ
Sbjct: 1685 FGIYSVREVMSVLRLFWSMDVDGSGSLSMTELQQYTPFFEKLGYHDLASVFQXXXXXXXXXXXXXXLLEICFHFATKVQLEAMLQLAKVGSSASGASSQSV--DAILSGASTSNLQVEQRRELLAIFRVFDHNGDGGVSMQEIMEALRVDDDDVMAAVMTREHERADRGHASRAAGPVVASGITREDVEQLYREFDADQDATLDFDEFVAVMQ 1895          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig89711.20799.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A5A8DGM4_CAFRO8.330e-2733.89Uncharacterized protein n=1 Tax=Cafeteria roenberg... [more]
A0A5A8CB72_CAFRO8.470e-2733.89Uncharacterized protein n=5 Tax=Sar TaxID=2698737 ... [more]
A0A421GQD1_9STRA3.900e-1329.95Uncharacterized protein n=3 Tax=Phytophthora kerno... [more]
T0RWW2_SAPDV2.350e-1235.97Uncharacterized protein n=2 Tax=Saprolegnia TaxID=... [more]
A0A1V9ZD18_9STRA9.320e-1132.87Uncharacterized protein n=1 Tax=Thraustotheca clav... [more]
A0A6A3X208_9STRA2.420e-1023.29Uncharacterized protein n=3 Tax=Phytophthora fraga... [more]
A0A6A3IQF4_9STRA1.500e-922.83Uncharacterized protein n=2 Tax=Phytophthora TaxID... [more]
A0A2D4CDJ0_PYTIN6.710e-927.14Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
A0A067BCI8_SAPPC4.870e-831.94Uncharacterized protein n=1 Tax=Saprolegnia parasi... [more]
A0A662WFT7_9STRA7.920e-823.47Uncharacterized protein n=1 Tax=Nothophytophthora ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig89711contigM-pyrifera_M_contig89711:135..671 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score84.0
Seed ortholog evalue8e-14
Seed eggNOG ortholog157072.XP_008863187.1
KEGG koko:K12862
KEGG Pathwayko03040,map03040
KEGG ModuleM00353,M00355
Hectar predicted targeting categoryother localisation
EggNOG free text desc.mRNA splicing, via spliceosome
EggNOG OGsKOG0285@1,KOG0285@2759
COG Functional cat.L
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko03041,ko03400
Exons1
Model size537
Cds size537
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816808.256312-CDS-M-pyrifera_M_contig89711:134..6711622816808.256312-CDS-M-pyrifera_M_contig89711:134..671Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89711 135..671 -
1692278190.7511601-CDS-M-pyrifera_M_contig89711:134..6711692278190.7511601-CDS-M-pyrifera_M_contig89711:134..671Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89711 135..671 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig89711.20799.1prot_M-pyrifera_M_contig89711.20799.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig89711 135..671 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig89711.20799.1

>prot_M-pyrifera_M_contig89711.20799.1 ID=prot_M-pyrifera_M_contig89711.20799.1|Name=mRNA_M-pyrifera_M_contig89711.20799.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=179bp
FGPYSALEVIRARRVFEGLDINGSGDVDMEELKGSSEFRQMYSDERIAVM
FDTIDADSSGQISLQELFHVLFPMASRVLLKKMIEMTHLRRRLAARVKKE
TLRDELTDGQRAEIAAIFRAFDRDDDGTITVNEIKQTLKAQGELFADIWA
DLQLDTIFNRYDKDKSLSMDLTEFTSLMQ
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mRNA from alignment at M-pyrifera_M_contig89711:135..671-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig89711.20799.1 ID=mRNA_M-pyrifera_M_contig89711.20799.1|Name=mRNA_M-pyrifera_M_contig89711.20799.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=537bp|location=Sequence derived from alignment at M-pyrifera_M_contig89711:135..671- (Macrocystis pyrifera P11B4 male)
TTTGGACCATATTCAGCATTGGAAGTGATTCGAGCAAGGAGAGTGTTTGA AGGATTGGATATCAATGGAAGTGGTGATGTTGATATGGAGGAATTGAAAG GGTCAAGCGAATTTCGTCAAATGTACTCTGATGAACGGATTGCTGTCATG TTTGACACAATTGATGCCGATTCAAGTGGACAAATTTCCCTACAAGAACT CTTTCATGTACTCTTCCCAATGGCATCTCGAGTCTTGCTGAAGAAGATGA TTGAAATGACTCACTTGCGTCGTCGTCTTGCAGCTCGTGTGAAGAAAGAA ACCCTTCGAGATGAACTCACAGATGGGCAACGTGCAGAAATTGCTGCCAT TTTCCGTGCATTTGATCGTGATGATGATGGAACCATCACTGTCAACGAAA TCAAACAAACTCTCAAGGCACAAGGTGAATTGTTTGCAGACATTTGGGCT GATCTCCAACTTGACACCATCTTCAACCGCTACGACAAAGACAAGAGCCT CTCGATGGACCTGACAGAGTTTACCTCTCTCATGCAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig89711:135..671-

>mRNA_M-pyrifera_M_contig89711.20799.1 ID=mRNA_M-pyrifera_M_contig89711.20799.1|Name=mRNA_M-pyrifera_M_contig89711.20799.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1074bp|location=Sequence derived from alignment at M-pyrifera_M_contig89711:135..671- (Macrocystis pyrifera P11B4 male)
TTTGGACCATATTCAGCATTGGAAGTGATTCGAGCAAGGAGAGTGTTTGA
AGGATTGGATATCAATGGAAGTGGTGATGTTGATATGGAGGAATTGAAAG
GGTCAAGCGAATTTCGTCAAATGTACTCTGATGAACGGATTGCTGTCATG
TTTGACACAATTGATGCCGATTCAAGTGGACAAATTTCCCTACAAGAACT
CTTTCATGTACTCTTCCCAATGGCATCTCGAGTCTTGCTGAAGAAGATGA
TTGAAATGACTCACTTGCGTCGTCGTCTTGCAGCTCGTGTGAAGAAAGAA
ACCCTTCGAGATGAACTCACAGATGGGCAACGTGCAGAAATTGCTGCCAT
TTTCCGTGCATTTGATCGTGATGATGATGGAACCATCACTGTCAACGAAA
TCAAACAAACTCTCAAGGCACAAGGTGAATTGTTTGCAGACATTTGGGCT
GATCTCCAACTTGACACCATCTTCAACCGCTACGACAAAGACAAGAGCCT
CTCGATGGACCTGACAGAGTTTACCTCTCTCATGCAATTTGGACCATATT
CAGCATTGGAAGTGATTCGAGCAAGGAGAGTGTTTGAAGGATTGGATATC
AATGGAAGTGGTGATGTTGATATGGAGGAATTGAAAGGGTCAAGCGAATT
TCGTCAAATGTACTCTGATGAACGGATTGCTGTCATGTTTGACACAATTG
ATGCCGATTCAAGTGGACAAATTTCCCTACAAGAACTCTTTCATGTACTC
TTCCCAATGGCATCTCGAGTCTTGCTGAAGAAGATGATTGAAATGACTCA
CTTGCGTCGTCGTCTTGCAGCTCGTGTGAAGAAAGAAACCCTTCGAGATG
AACTCACAGATGGGCAACGTGCAGAAATTGCTGCCATTTTCCGTGCATTT
GATCGTGATGATGATGGAACCATCACTGTCAACGAAATCAAACAAACTCT
CAAGGCACAAGGTGAATTGTTTGCAGACATTTGGGCTGATCTCCAACTTG
ACACCATCTTCAACCGCTACGACAAAGACAAGAGCCTCTCGATGGACCTG
ACAGAGTTTACCTCTCTCATGCAA
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