mRNA_M-pyrifera_M_contig89487.20745.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig89487.20745.1
Unique NamemRNA_M-pyrifera_M_contig89487.20745.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Match: A0A1X2HDJ8_SYNRA (WD40-repeat-containing domain protein n=1 Tax=Syncephalastrum racemosum TaxID=13706 RepID=A0A1X2HDJ8_SYNRA)

HSP 1 Score: 148 bits (373), Expect = 4.260e-38
Identity = 75/182 (41.21%), Postives = 108/182 (59.34%), Query Frame = 1
Query:    1 HHRVLRRVLDFFSERGDVQMCVTVSRVEGKGSLDLVGSLQIERWTDAYVDLLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQPPLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFVWCQGCGHGGHMEHLRQWFATHTTCPAGCGHHC 546
            H  V+  +LD+++E+GDVQMCVT+  V  K  L+ V   ++E W  +Y+DLLHRF+LWT A+ I+    VPRIR++N++AT +++AC +C K +   +Q                           CD C +     C +C   VRG++VWCQGC HGGH+EHL++WF T T C +GCGH C
Sbjct:  555 HENVVMDLLDYYTEQGDVQMCVTLYLVLQK-YLN-VPQTRLEDWFTSYLDLLHRFKLWTTATAIINACPVPRIREMNENATTINIACNNCFKLVIGSSQ-----------------------GSWACDKCHRLLN-TCTLCHQTVRGMYVWCQGCNHGGHLEHLQEWFKTETLCASGCGHTC 710          
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Match: A0A8H7S2I7_9FUNG (Uncharacterized protein n=1 Tax=Mucor circinatus TaxID=2054153 RepID=A0A8H7S2I7_9FUNG)

HSP 1 Score: 146 bits (369), Expect = 1.820e-37
Identity = 73/183 (39.89%), Postives = 102/183 (55.74%), Query Frame = 1
Query:    1 HHRVLRRVLDFFSERGDVQMCVTVSRVEGKGSLDLVGSLQIERWTDAYVDLLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQPPLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFVWCQGCGHGGHMEHLRQWFATHTTCPAGCGHHCT 549
            H  V+  ++D+++E GDVQMCVT+  V        V S ++E W  AY+DLLHRF+LWT A+ ++    + RIR+ N++AT +++AC +C K +   TQ                           CD C +     C +C   VRGL+VWCQGC HGGH+EH+R+WF     CP GCGH CT
Sbjct:  599 HQGVVDELMDYYTEEGDVQMCVTLYLVLRHHIQ--VNSNRLEDWFSAYIDLLHRFKLWTTATAVINACPIARIRERNENATTINIACNNCFKLVMGTTQ-----------------------GAWACDKCHRLLNP-CSICHQTVRGLYVWCQGCNHGGHLEHMREWFDNEDKCPTGCGHSCT 755          
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Match: A0A068RVV7_9FUNG (Wd repeat-containing protein 24 n=2 Tax=Lichtheimia TaxID=688353 RepID=A0A068RVV7_9FUNG)

HSP 1 Score: 145 bits (365), Expect = 5.610e-37
Identity = 74/182 (40.66%), Postives = 103/182 (56.59%), Query Frame = 1
Query:    1 HHRVLRRVLDFFSERGDVQMCVTVSRVEGKGSLDLVGSLQIERWTDAYVDLLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQPPLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFVWCQGCGHGGHMEHLRQWFATHTTCPAGCGHHC 546
            H  V+  +L++++E+GDVQMCVT+  V        V   QIE W  AY+DLL RF+LWT A+ ++    V RIR+ N+++T +++AC +C K         LV              S +      CD C +   G C +C   VRGL+VWCQGC HGGH+EH+R+WF+    CP GCGH C
Sbjct:  571 HEGVIHELLEYYTEQGDVQMCVTLYLVLQNHIT--VNHDQIEDWFSAYIDLLQRFKLWTTAAAVINACPVSRIRERNENSTTINIACNNCFK---------LV--------------SGSAQGAWACDKCHQLLNG-CSICHQTVRGLYVWCQGCNHGGHLEHMREWFSNEPLCPTGCGHSC 726          
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Match: A0A168PE20_ABSGL (Uncharacterized protein n=1 Tax=Absidia glauca TaxID=4829 RepID=A0A168PE20_ABSGL)

HSP 1 Score: 145 bits (365), Expect = 6.240e-37
Identity = 73/182 (40.11%), Postives = 104/182 (57.14%), Query Frame = 1
Query:    1 HHRVLRRVLDFFSERGDVQMCVTVSRVEGKGSLDLVGSLQIERWTDAYVDLLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQPPLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFVWCQGCGHGGHMEHLRQWFATHTTCPAGCGHHC 546
            H  V+  +LD+++E+ DVQMCVT+  V  K     V   ++E W D+Y+D+LHRF+LW+ A+ I+    +PRI++ NQ AT +++AC++C K         LV                T      CD C +     C +C   V+GL+VWCQGC HGGH+EH+R WFA    CP GCGH+C
Sbjct:  593 HDNVVMELLDYYTEQCDVQMCVTLYLVLQK--YIRVNDERLENWFDSYIDMLHRFKLWSAATAIINACKIPRIQERNQMATTINIACSNCFK---------LV--------------LGTAQGAWACDKCHRLLNP-CSICNQTVKGLYVWCQGCNHGGHLEHMRSWFAKEKQCPTGCGHNC 748          
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Match: A0A1Y1Z6N6_9FUNG (WD40 repeat-like protein n=1 Tax=Basidiobolus meristosporus CBS 931.73 TaxID=1314790 RepID=A0A1Y1Z6N6_9FUNG)

HSP 1 Score: 141 bits (355), Expect = 1.220e-35
Identity = 69/188 (36.70%), Postives = 105/188 (55.85%), Query Frame = 1
Query:    1 HHRVLRRVLDFFSERGDVQMCVTV-----SRVEGKGSLDLVGSLQIERWTDAYVDLLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQPPLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFVWCQGCGHGGHMEHLRQWFATHTTCPAGCGHHCT 549
            H  +L  ++ ++ E+GDVQMCVT+       ++ K +L+       E+W  +YVDLLHRF+LW+ A+ ++++  +  +R+ NQ++T +  +C  C KPL                +S H           VCD C K     C +C   VRGL+VWCQGC HGGH+ H++ WF  ++TCP GCGH C+
Sbjct:  540 HENILENLMKYYGEKGDVQMCVTLLIVLEKHIQHKFALE-------EQWFWSYVDLLHRFKLWSAAAAVISKCKIASVRQRNQESTTVYTSCNYCFKPL---------------LNSKHG--------FWVCDKCRKLLNP-CTICHQTVRGLYVWCQGCSHGGHLLHMQDWFRENSTCPTGCGHQCS 696          
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Match: A0A162UXF6_PHYB8 (Uncharacterized protein n=1 Tax=Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555) TaxID=763407 RepID=A0A162UXF6_PHYB8)

HSP 1 Score: 141 bits (355), Expect = 1.340e-35
Identity = 69/182 (37.91%), Postives = 101/182 (55.49%), Query Frame = 1
Query:    1 HHRVLRRVLDFFSERGDVQMCVTVSRVEGKGSLDLVGSLQIERWTDAYVDLLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQPPLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFVWCQGCGHGGHMEHLRQWFATHTTCPAGCGHHC 546
            H  V+  +LD+++E+GDVQMCVT+  V  +     +   ++E W  +Y+DLLHRF+LW+PA+ ++    V R+R+ N++AT + +AC  C K +   T                            CD C +     C +C   VRGL+VWCQGC HGGH+EH+R+WF+  T C  GCGH C
Sbjct:  575 HEGVVMELLDYYTEQGDVQMCVTLYLVLER--YIHIDDNRVEDWFTSYIDLLHRFKLWSPATVMIKACRVKRVRERNENATTIHIACNHCFKVVMGTTN-----------------------GSWACDKCRRLLNP-CSICHQTVRGLYVWCQGCNHGGHLEHMREWFSKETLCATGCGHTC 730          
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Match: A0A1C7NA51_9FUNG (WD repeat-containing protein 24 n=1 Tax=Choanephora cucurbitarum TaxID=101091 RepID=A0A1C7NA51_9FUNG)

HSP 1 Score: 140 bits (352), Expect = 3.070e-35
Identity = 70/182 (38.46%), Postives = 103/182 (56.59%), Query Frame = 1
Query:    1 HHRVLRRVLDFFSERGDVQMCVTVSRVEGKGSLDLVGSLQIERWTDAYVDLLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQPPLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFVWCQGCGHGGHMEHLRQWFATHTTCPAGCGHHC 546
            H  V+  +L +++++GD+QMCVT+  V  K     + S ++E W  AY+DLLHRF+LWT A++IM    +  +R+ N++AT +++AC +C K         LV              + T      CD C +     C +C   VRGL+VWCQGC HGGH+ H+R+WFA  + C  GCGH C
Sbjct:  533 HESVIMDLLQYYTDQGDIQMCVTLYLVLEK--YIEIDSERVEDWFTAYIDLLHRFKLWTTATSIMKACSIQAVRERNENATTVNIACNTCFK---------LV--------------NGTSNGAWACDKCHRLLNP-CTICHQTVRGLYVWCQGCNHGGHLTHMREWFAIESLCATGCGHTC 688          
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Match: A0A1X2ICG5_9FUNG (WD40-repeat-containing domain protein n=1 Tax=Absidia repens TaxID=90262 RepID=A0A1X2ICG5_9FUNG)

HSP 1 Score: 140 bits (352), Expect = 3.820e-35
Identity = 69/182 (37.91%), Postives = 101/182 (55.49%), Query Frame = 1
Query:    1 HHRVLRRVLDFFSERGDVQMCVTVSRVEGKGSLDLVGSLQIERWTDAYVDLLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQPPLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFVWCQGCGHGGHMEHLRQWFATHTTCPAGCGHHC 546
            H  V+  +LD+++E+ DVQMCVT+  V    S   +   ++E W  +Y+D+LHRF+LW+ A+ I+    +PRI++ NQ AT +++AC +C K +   TQ                           CD C +     C +C   V+GL+VWCQGC HGGH+EH+R WF+    CP GCGH C
Sbjct:  616 HDNVIMELLDYYTEQCDVQMCVTLYLV--LQSYIRINDERLEDWFSSYIDMLHRFKLWSAATAIINACKIPRIQERNQMATTINIACNNCFKLVLGTTQ-----------------------GAWACDKCHRLLNP-CSICHQTVKGLYVWCQGCNHGGHLEHMRAWFSKEKQCPTGCGHSC 771          
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Match: A0A8H7VWQ6_9FUNG (Uncharacterized protein n=1 Tax=Thamnidium elegans TaxID=101142 RepID=A0A8H7VWQ6_9FUNG)

HSP 1 Score: 139 bits (350), Expect = 5.650e-35
Identity = 72/182 (39.56%), Postives = 103/182 (56.59%), Query Frame = 1
Query:    1 HHRVLRRVLDFFSERGDVQMCVTVSRVEGKGSLDLVGSLQIERWTDAYVDLLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQPPLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFVWCQGCGHGGHMEHLRQWFATHTTCPAGCGHHC 546
            H  V+  +L++++E+GDVQMCVT+  V  K  L+ + + +IE W  AY+DLLHRF+LW+ A+ I+    V  +R+ N++AT +++AC +C K         LV              + T      CD C K     C +C   VRGL+VWCQGC HGGH+ H++ WFA    C  GCGH C
Sbjct:  526 HESVVMELLNYYTEQGDVQMCVTLYLVLEK-YLN-IDNERIEDWFTAYIDLLHRFKLWSTATAIIKACKVQNVRERNENATTINIACNTCFK---------LV--------------NGTSSGSWACDKCHKLLNS-CTICHQTVRGLYVWCQGCNHGGHLSHMKDWFADEQQCATGCGHAC 681          
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Match: A0A397SZI5_9GLOM (WD40-repeat-containing domain protein n=1 Tax=Glomus cerebriforme TaxID=658196 RepID=A0A397SZI5_9GLOM)

HSP 1 Score: 137 bits (346), Expect = 2.380e-34
Identity = 60/180 (33.33%), Postives = 103/180 (57.22%), Query Frame = 1
Query:   10 VLRRVLDFFSERGDVQMCVTVSRVEGKGSLDLVGSLQIERWTDAYVDLLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQPPLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFVWCQGCGHGGHMEHLRQWFATHTTCPAGCGHHCT 549
            ++ ++LD+++E+GDVQMCVT+  V   G    +   ++E+W  +Y++LLHRF+LW  A+ I++   +P +  +NQ +T +   C +C +P+                        ++   + +CD C K     C +C   V+GL+ WCQGC HGGH+ H+++WF+ +  CP GCGH+C+
Sbjct:  608 IMSQLLDYYAEQGDVQMCVTLMLV--LGDRIKISQERVEQWFFSYIELLHRFQLWCAATYIISSCRIPSVEMMNQQSTTIYTTCNNCFRPI-----------------------LNSRCRYWICDKCRKMLNP-CSICHNTVKGLYTWCQGCSHGGHLAHMKEWFSVNDECPTGCGHNCS 761          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig89487.20745.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A1X2HDJ8_SYNRA4.260e-3841.21WD40-repeat-containing domain protein n=1 Tax=Sync... [more]
A0A8H7S2I7_9FUNG1.820e-3739.89Uncharacterized protein n=1 Tax=Mucor circinatus T... [more]
A0A068RVV7_9FUNG5.610e-3740.66Wd repeat-containing protein 24 n=2 Tax=Lichtheimi... [more]
A0A168PE20_ABSGL6.240e-3740.11Uncharacterized protein n=1 Tax=Absidia glauca Tax... [more]
A0A1Y1Z6N6_9FUNG1.220e-3536.70WD40 repeat-like protein n=1 Tax=Basidiobolus meri... [more]
A0A162UXF6_PHYB81.340e-3537.91Uncharacterized protein n=1 Tax=Phycomyces blakesl... [more]
A0A1C7NA51_9FUNG3.070e-3538.46WD repeat-containing protein 24 n=1 Tax=Choanephor... [more]
A0A1X2ICG5_9FUNG3.820e-3537.91WD40-repeat-containing domain protein n=1 Tax=Absi... [more]
A0A8H7VWQ6_9FUNG5.650e-3539.56Uncharacterized protein n=1 Tax=Thamnidium elegans... [more]
A0A397SZI5_9GLOM2.380e-3433.33WD40-repeat-containing domain protein n=1 Tax=Glom... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig89487contigM-pyrifera_M_contig89487:205..753 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score139.8
Seed ortholog evalue1.3e-30
Seed eggNOG ortholog588596.U9U7V4
Preferred nameRTC1
KEGG koko:K20408
KEGG Pathwayko04150,map04150
Hectar predicted targeting categoryother localisation
GOsGO:0000306,GO:0000322,GO:0000323,GO:0000324,GO:0000329,GO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005773,GO:0005774,GO:0005829,GO:0006464,GO:0006807,GO:0008150,GO:0008152,GO:0009966,GO:0009967,GO:0009987,GO:0010646,GO:0010647,GO:0016020,GO:0016740,GO:0019538,GO:0019787,GO:0019898,GO:0023051,GO:0023056,GO:0031090,GO:0031312,GO:0032006,GO:0032008,GO:0032991,GO:0035859,GO:0036211,GO:0043170,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043412,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044422,GO:0044424,GO:0044425,GO:0044437,GO:0044444,GO:0044446,GO:0044464,GO:0048518,GO:0048522,GO:0048583,GO:0048584,GO:0050789,GO:0050794,GO:0061659,GO:0065007,GO:0070647,GO:0071704,GO:0097042,GO:0098588,GO:0098805,GO:0098852,GO:0140096,GO:1901564,GO:1902531,GO:1902533,GO:1903432,GO:1904263
EggNOG free text desc.Wd repeat protein
EggNOG OGs38HMY@33154,3NWBI@4751,KOG0269@1,KOG0269@2759
Ec32 ortholog descriptionconserved unknown protein
Ec32 orthologEc-27_005460.1
COG Functional cat.Q
Best tax levelFungi
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001
Exons1
Model size549
Cds size549
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816805.218697-CDS-M-pyrifera_M_contig89487:204..7531622816805.218697-CDS-M-pyrifera_M_contig89487:204..753Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89487 205..753 +
1692278188.9189355-CDS-M-pyrifera_M_contig89487:204..7531692278188.9189355-CDS-M-pyrifera_M_contig89487:204..753Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89487 205..753 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig89487.20745.1prot_M-pyrifera_M_contig89487.20745.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig89487 205..753 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig89487.20745.1

>prot_M-pyrifera_M_contig89487.20745.1 ID=prot_M-pyrifera_M_contig89487.20745.1|Name=mRNA_M-pyrifera_M_contig89487.20745.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=183bp
HHRVLRRVLDFFSERGDVQMCVTVSRVEGKGSLDLVGSLQIERWTDAYVD
LLHRFRLWTPASTIMARAVVPRIRKLNQDATKLSLACASCAKPLPDYTQP
PLVPLPHEEASSPHSPDSSTEVPHIVCDNCGKTQRGICVVCRIPVRGLFV
WCQGCGHGGHMEHLRQWFATHTTCPAGCGHHCT
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mRNA from alignment at M-pyrifera_M_contig89487:205..753+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig89487.20745.1 ID=mRNA_M-pyrifera_M_contig89487.20745.1|Name=mRNA_M-pyrifera_M_contig89487.20745.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=549bp|location=Sequence derived from alignment at M-pyrifera_M_contig89487:205..753+ (Macrocystis pyrifera P11B4 male)
CATCATAGAGTATTGCGTCGTGTGTTGGATTTCTTCAGCGAGCGAGGGGA TGTGCAGATGTGTGTGACTGTGAGTCGTGTGGAAGGGAAGGGGAGTTTGG ATTTGGTGGGGAGTCTTCAGATCGAGCGGTGGACAGATGCGTATGTGGAC CTGTTGCATCGCTTTCGCTTGTGGACTCCTGCTTCTACGATCATGGCTCG GGCAGTGGTTCCTCGTATTCGCAAGTTGAACCAAGATGCGACCAAGCTGT CCTTAGCGTGTGCTTCTTGTGCCAAGCCCCTCCCTGACTATACACAGCCT CCACTCGTGCCACTCCCTCACGAAGAAGCTTCCTCTCCACATTCCCCGGA TTCTTCCACAGAAGTACCACACATCGTCTGTGACAATTGTGGGAAAACAC AACGAGGGATCTGTGTGGTATGCAGAATCCCAGTCAGGGGCCTCTTCGTG TGGTGTCAAGGGTGTGGGCATGGAGGACACATGGAGCACCTCAGGCAATG GTTTGCCACGCATACCACTTGCCCAGCGGGGTGTGGTCACCACTGTACC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig89487:205..753+

>mRNA_M-pyrifera_M_contig89487.20745.1 ID=mRNA_M-pyrifera_M_contig89487.20745.1|Name=mRNA_M-pyrifera_M_contig89487.20745.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1098bp|location=Sequence derived from alignment at M-pyrifera_M_contig89487:205..753+ (Macrocystis pyrifera P11B4 male)
CATCATAGAGTATTGCGTCGTGTGTTGGATTTCTTCAGCGAGCGAGGGGA
TGTGCAGATGTGTGTGACTGTGAGTCGTGTGGAAGGGAAGGGGAGTTTGG
ATTTGGTGGGGAGTCTTCAGATCGAGCGGTGGACAGATGCGTATGTGGAC
CTGTTGCATCGCTTTCGCTTGTGGACTCCTGCTTCTACGATCATGGCTCG
GGCAGTGGTTCCTCGTATTCGCAAGTTGAACCAAGATGCGACCAAGCTGT
CCTTAGCGTGTGCTTCTTGTGCCAAGCCCCTCCCTGACTATACACAGCCT
CCACTCGTGCCACTCCCTCACGAAGAAGCTTCCTCTCCACATTCCCCGGA
TTCTTCCACAGAAGTACCACACATCGTCTGTGACAATTGTGGGAAAACAC
AACGAGGGATCTGTGTGGTATGCAGAATCCCAGTCAGGGGCCTCTTCGTG
TGGTGTCAAGGGTGTGGGCATGGAGGACACATGGAGCACCTCAGGCAATG
GTTTGCCACGCATACCACTTGCCCAGCGGGGTGTGGTCACCACTGTACCC
ATCATAGAGTATTGCGTCGTGTGTTGGATTTCTTCAGCGAGCGAGGGGAT
GTGCAGATGTGTGTGACTGTGAGTCGTGTGGAAGGGAAGGGGAGTTTGGA
TTTGGTGGGGAGTCTTCAGATCGAGCGGTGGACAGATGCGTATGTGGACC
TGTTGCATCGCTTTCGCTTGTGGACTCCTGCTTCTACGATCATGGCTCGG
GCAGTGGTTCCTCGTATTCGCAAGTTGAACCAAGATGCGACCAAGCTGTC
CTTAGCGTGTGCTTCTTGTGCCAAGCCCCTCCCTGACTATACACAGCCTC
CACTCGTGCCACTCCCTCACGAAGAAGCTTCCTCTCCACATTCCCCGGAT
TCTTCCACAGAAGTACCACACATCGTCTGTGACAATTGTGGGAAAACACA
ACGAGGGATCTGTGTGGTATGCAGAATCCCAGTCAGGGGCCTCTTCGTGT
GGTGTCAAGGGTGTGGGCATGGAGGACACATGGAGCACCTCAGGCAATGG
TTTGCCACGCATACCACTTGCCCAGCGGGGTGTGGTCACCACTGTACC
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