mRNA_M-pyrifera_M_contig8945.20729.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig8945.20729.1
Unique NamemRNA_M-pyrifera_M_contig8945.20729.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Match: D8LMF2_ECTSI (Similar to Isochorismate Synthase n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LMF2_ECTSI)

HSP 1 Score: 163 bits (413), Expect = 2.070e-44
Identity = 92/126 (73.02%), Postives = 98/126 (77.78%), Query Frame = 1
Query:    1 LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVE----------SAKGVFSGARGGGRLSDVASNRGASGIDGVVSSAIGYAAGLGAPVTLLIGDMATLHDLGSLHALRGLDAT-SHPVTVVCVNNGG 345
            LTEPFV+WCVSR IDPAAGLFLSASMPCRDVE            +G      GG RLSDVASNRGASGIDGV SSA+GYAAGL +  TLLIGDMATLHDLGSLHAL+GLDA  S  VT+VCVNNGG
Sbjct: 1394 LTEPFVAWCVSRHIDPAAGLFLSASMPCRDVEVFADSGLGDLDGRGEEDRGSGGRRLSDVASNRGASGIDGVFSSAMGYAAGLRSAATLLIGDMATLHDLGSLHALKGLDAAGSCSVTMVCVNNGG 1519          
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Match: A0A6H5JV35_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JV35_9PHAE)

HSP 1 Score: 152 bits (385), Expect = 1.210e-40
Identity = 93/150 (62.00%), Postives = 99/150 (66.00%), Query Frame = 1
Query:    1 LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVE----------SAKGVFSGARGGGRLSDVASNRGASGIDGVVSSAIG------------------------YAAGLGAPVTLLIGDMATLHDLGSLHALRGLDAT-SHPVTVVCVNNGG 345
            LTEPFV+WCVSR IDPAAGLFLSASMPCRDVE            +G      GG RLSDVASNRGASGIDGV SSA+G                        YAAGL +  TLLIGDMATLHDLGSLHAL+GLDA  S PVT+VCVNNGG
Sbjct: 1112 LTEPFVAWCVSRHIDPAAGLFLSASMPCRDVEVFADSGLGDLDGRGEEDRGSGGRRLSDVASNRGASGIDGVSSSAMGCVPYARNTRRPMALPGFWHVLLLRYAAGLRSAATLLIGDMATLHDLGSLHALKGLDAAGSCPVTMVCVNNGG 1261          
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Match: A0A835ZAA5_9STRA (Thiamine diphosphate-binding protein (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZAA5_9STRA)

HSP 1 Score: 129 bits (325), Expect = 7.610e-35
Identity = 70/115 (60.87%), Postives = 84/115 (73.04%), Query Frame = 1
Query:    1 LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVESAKGVFSGARGGGRLSDVASNRGASGIDGVVSSAIGYAAGLGAPVTLLIGDMATLHDLGSLHALRGLDATSHPVTVVCVNNGG 345
            L EP ++ CVS  + P AGLFLS SMPCRD+++    F+   G G L  VA NRGA+GIDGVVSSA+GYA GL  PVTLLIGDMA LHD+ +LH LR +    HP+T+VCVNNGG
Sbjct:    7 LVEPAIARCVSAHLSPGAGLFLSNSMPCRDMDT----FAQHSGPGNLCHVAMNRGANGIDGVVSSALGYAVGLRRPVTLLIGDMALLHDVNALHLLRQIP---HPITIVCVNNGG 114          
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Match: A0A4D9D2W1_9STRA (MR_MLE domain-containing protein n=6 Tax=Monodopsidaceae TaxID=425072 RepID=A0A4D9D2W1_9STRA)

HSP 1 Score: 112 bits (281), Expect = 1.290e-26
Identity = 68/122 (55.74%), Postives = 82/122 (67.21%), Query Frame = 1
Query:    1 LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVESAKGVFSGARGGGR---LSDVASNRGASGIDGVVSSAIGYAAGLGAPVTLLIGDMATLHDLGSLHALRGLDATSH----PVTVVCVNNGG 345
            L EP  +  V+R +    GLFLS SMP RD+E+   + S          L+ VA NRGASGIDG++SSA+GYAAGLG PVTLL+GDMA LHDL +LHAL G  A++     PVTVV VNNGG
Sbjct:  986 LNEPMTAHLVARLLPAGHGLFLSNSMPVRDMETFATLASTTAAPSAPAPLASVAMNRGASGIDGILSSAMGYAAGLGKPVTLLVGDMACLHDLNALHALSGASASASAPDTPVTVVLVNNGG 1107          
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Match: A0A7S1MXA8_HEMAN (Hypothetical protein (Fragment) n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1MXA8_HEMAN)

HSP 1 Score: 103 bits (256), Expect = 5.630e-26
Identity = 62/115 (53.91%), Postives = 74/115 (64.35%), Query Frame = 1
Query:    1 LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVESAKGVFSGARGGGRLSDVASNRGASGIDGVVSSAIGYAAGLGAPVTLLIGDMATLHDLGSLHALRGLDATSHPVTVVCVNNGG 345
            L EP+V++  + ++     LFLSASMP RD +    +F           VASNRGASGIDGVVSSA+GYA GL  PVTLLIGD A LHDLG+L  LR    +  PV V+ VNNGG
Sbjct:    7 LDEPYVAFRTTTELREGGALFLSASMPVRDAD----MFLNPSARNPAVHVASNRGASGIDGVVSSALGYAQGLARPVTLLIGDGAALHDLGALRCLRD---SEQPVCVIVVNNGG 114          
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Match: A0A3M1NL11_9BACT (TPP_enzyme_C domain-containing protein (Fragment) n=1 Tax=Calditrichaeota bacterium TaxID=2212469 RepID=A0A3M1NL11_9BACT)

HSP 1 Score: 100 bits (249), Expect = 5.190e-24
Identity = 56/115 (48.70%), Postives = 79/115 (68.70%), Query Frame = 1
Query:    1 LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVESAKGVFSGARGGGRLSDVASNRGASGIDGVVSSAIGYAAGLGAPVTLLIGDMATLHDLGSLHALRGLDATSHPVTVVCVNNGG 345
            LTEP ++W VSR I   + LFL++SMP RD +    +++   G   ++ VA+NRGASGIDG ++SA+GY+ GL  PV  ++GD+A LHD+ SLH LR L   SHP+ +V +NN G
Sbjct:    6 LTEPGLAWTVSRSIPEDSALFLASSMPVRDFD----MYAFPSG---ITRVAANRGASGIDGTIASAVGYSRGLETPVIAVLGDLALLHDMNSLHLLREL---SHPMKIVVINNHG 110          
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Match: A0A1V0DD88_9BACT (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase n=2 Tax=Bacteroidetes TaxID=976 RepID=A0A1V0DD88_9BACT)

HSP 1 Score: 102 bits (255), Expect = 3.610e-23
Identity = 63/115 (54.78%), Postives = 75/115 (65.22%), Query Frame = 1
Query:    1 LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVESAKGVFSGARGGGRLSDVASNRGASGIDGVVSSAIGYAAGLGAPVTLLIGDMATLHDLGSLHALRGLDATSHPVTVVCVNNGG 345
            L+EP V+  V+R + P  GLFL++SMP RD +        A GG     VA+NRGASGIDG V+SA G+AAGL  PVTLLIGD+A LHDL SL  LR L     PV VV +NN G
Sbjct:  390 LSEPLVARLVTRHLPPEHGLFLASSMPVRDADMY------AVGGAAAVPVAANRGASGIDGTVASAAGFAAGLARPVTLLIGDLALLHDLNSLALLRRLPV---PVVVVAINNDG 495          
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Match: A0A1Q7ZRX4_9CYAN (TPP_enzyme_C domain-containing protein n=1 Tax=Cyanobacteria bacterium 13_1_40CM_2_61_4 TaxID=1805101 RepID=A0A1Q7ZRX4_9CYAN)

HSP 1 Score: 99.0 bits (245), Expect = 3.990e-23
Identity = 61/115 (53.04%), Postives = 76/115 (66.09%), Query Frame = 1
Query:    1 LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVESAKGVFSGARGGGRLSDVASNRGASGIDGVVSSAIGYAAGLGAPVTLLIGDMATLHDLGSLHALRGLDATSHPVTVVCVNNGG 345
            L+EP V+  VS++I    GLFL +SMP RD+ +       A  GG  + VA+NRGASGIDG V+SA G+A GL   VTLLIGD+A LHDL SL   R LDA   P+T+V +NN G
Sbjct:   51 LSEPLVARLVSQEIGAERGLFLGSSMPVRDMNTF------AAPGGPSTRVAANRGASGIDGTVASATGFAQGLNHRVTLLIGDLALLHDLNSLSLTRSLDA---PMTIVVLNNNG 156          
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Match: UPI000E5B8BEE (2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-carboxylic-acid synthase n=1 Tax=Rubrobacter indicoceani TaxID=2051957 RepID=UPI000E5B8BEE)

HSP 1 Score: 102 bits (253), Expect = 6.730e-23
Identity = 61/115 (53.04%), Postives = 76/115 (66.09%), Query Frame = 1
Query:    1 LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVESAKGVFSGARGGGRLSDVASNRGASGIDGVVSSAIGYAAGLGAPVTLLIGDMATLHDLGSLHALRGLDATSHPVTVVCVNNGG 345
            L EPFV+  V RD+   +GL +++SMP RDV+S    ++G R       VA+NRGASGIDG V++A G+A G G  VT+LIGD+A LHDL SL  LRGL     PVT V VNN G
Sbjct:  389 LAEPFVARAVMRDMPSGSGLVVASSMPVRDVDS----YAGLRADAEYIPVAANRGASGIDGTVATAAGFALGSGRAVTVLIGDLALLHDLNSLAMLRGL-----PVTFVVVNNDG 494          
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Match: A0A523YYJ1_9BACT (2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase (Fragment) n=1 Tax=candidate division Zixibacteria bacterium TaxID=2053527 RepID=A0A523YYJ1_9BACT)

HSP 1 Score: 99.0 bits (245), Expect = 7.210e-22
Identity = 57/115 (49.57%), Postives = 77/115 (66.96%), Query Frame = 1
Query:    1 LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVESAKGVFSGARGGGRLSDVASNRGASGIDGVVSSAIGYAAGLGAPVTLLIGDMATLHDLGSLHALRGLDATSHPVTVVCVNNGG 345
            L+EP V+  +S++I P +GLF+S SMP RD++            G +  VA NRGASGIDG+++SA G+A GL  PVTLLIGD+A LHD+ SL  L+ L A  HP+ +V +NN G
Sbjct:  319 LSEPAVARLISQNIRPDSGLFVSNSMPIRDMDMYADPH------GPVVTVACNRGASGIDGIIASATGFACGLNKPVTLLIGDLAFLHDVNSLALLKSL-AHPHPLVIVVINNDG 426          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig8945.20729.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8LMF2_ECTSI2.070e-4473.02Similar to Isochorismate Synthase n=1 Tax=Ectocarp... [more]
A0A6H5JV35_9PHAE1.210e-4062.00Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A835ZAA5_9STRA7.610e-3560.87Thiamine diphosphate-binding protein (Fragment) n=... [more]
A0A4D9D2W1_9STRA1.290e-2655.74MR_MLE domain-containing protein n=6 Tax=Monodopsi... [more]
A0A7S1MXA8_HEMAN5.630e-2653.91Hypothetical protein (Fragment) n=1 Tax=Hemiselmis... [more]
A0A3M1NL11_9BACT5.190e-2448.70TPP_enzyme_C domain-containing protein (Fragment) ... [more]
A0A1V0DD88_9BACT3.610e-2354.782-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1... [more]
A0A1Q7ZRX4_9CYAN3.990e-2353.04TPP_enzyme_C domain-containing protein n=1 Tax=Cya... [more]
UPI000E5B8BEE6.730e-2353.042-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-... [more]
A0A523YYJ1_9BACT7.210e-2249.572-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig8945contigM-pyrifera_M_contig8945:491..1036 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score165.2
Seed ortholog evalue1.8e-38
Seed eggNOG ortholog2880.D8LMF2
KEGG rclassRC00588,RC01053,RC02148,RC02186,RC02475
KEGG koko:K02552,ko:K14759
KEGG ReactionR01717,R04031,R08165,R08166
KEGG Pathwayko00130,ko01053,ko01100,ko01110,ko01130,map00130,map01053,map01100,map01110,map01130
KEGG ModuleM00116
Hectar predicted targeting categoryother localisation
EggNOG free text desc.anthranilate synthase activity
EggNOG OGsCOG0147@1,COG0596@1,KOG1223@2759,KOG2382@2759
EC2.2.1.9,4.2.1.113,4.2.99.20,5.4.4.2
COG Functional cat.EH
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons2
Model size345
Cds size345
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816804.492291-CDS-M-pyrifera_M_contig8945:490..7371622816804.492291-CDS-M-pyrifera_M_contig8945:490..737Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8945 491..737 -
1692278188.530295-CDS-M-pyrifera_M_contig8945:490..7371692278188.530295-CDS-M-pyrifera_M_contig8945:490..737Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8945 491..737 -
1622816804.5098217-CDS-M-pyrifera_M_contig8945:938..10361622816804.5098217-CDS-M-pyrifera_M_contig8945:938..1036Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8945 939..1036 -
1692278188.5481303-CDS-M-pyrifera_M_contig8945:938..10361692278188.5481303-CDS-M-pyrifera_M_contig8945:938..1036Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8945 939..1036 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig8945.20729.1prot_M-pyrifera_M_contig8945.20729.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig8945 491..1036 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig8945.20729.1

>prot_M-pyrifera_M_contig8945.20729.1 ID=prot_M-pyrifera_M_contig8945.20729.1|Name=mRNA_M-pyrifera_M_contig8945.20729.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=115bp
LTEPFVSWCVSRDIDPAAGLFLSASMPCRDVESAKGVFSGARGGGRLSDV
ASNRGASGIDGVVSSAIGYAAGLGAPVTLLIGDMATLHDLGSLHALRGLD
ATSHPVTVVCVNNGG
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mRNA from alignment at M-pyrifera_M_contig8945:491..1036-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig8945.20729.1 ID=mRNA_M-pyrifera_M_contig8945.20729.1|Name=mRNA_M-pyrifera_M_contig8945.20729.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=546bp|location=Sequence derived from alignment at M-pyrifera_M_contig8945:491..1036- (Macrocystis pyrifera P11B4 male)
CTTACCGAGCCGTTCGTCTCCTGGTGCGTGTCCCGCGACATCGACCCCGC CGCCGGGCTGTTCCTGTCGGCGAGCATGCCGTGCCGCGACGTCGAGTCGT TCGCCGACAGCGGCCTGGACGGCGATCGTCGCCGCCGTCGAGTCCGGCGG CCACCATCACCGTTGTCGCCGCCGTCATCATCATCGTCGTCAGCTGACCG GCCTGTTGGAGACGACTGTCGGACACCCTCCCCCGTCGCCGCCACCACCG TCACCGGCTGCTGCTGGGATGGCGTTGGCAGTGGTGGTAACGGCGGCAGT GCCAAGGGCGTCTTCAGCGGCGCTAGGGGCGGCGGACGGCTTAGCGACGT CGCGTCAAATCGAGGTGCCAGCGGCATAGACGGTGTGGTGAGCTCGGCGA TCGGCTACGCGGCGGGGCTGGGGGCGCCGGTCACCCTGCTGATCGGTGAT ATGGCGACGCTGCACGACCTCGGCTCGCTGCACGCGCTACGGGGCCTGGA CGCGACGTCCCACCCAGTCACGGTGGTCTGCGTGAACAATGGAGGT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig8945:491..1036-

>mRNA_M-pyrifera_M_contig8945.20729.1 ID=mRNA_M-pyrifera_M_contig8945.20729.1|Name=mRNA_M-pyrifera_M_contig8945.20729.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=690bp|location=Sequence derived from alignment at M-pyrifera_M_contig8945:491..1036- (Macrocystis pyrifera P11B4 male)
CTTACCGAGCCGTTCGTCTCCTGGTGCGTGTCCCGCGACATCGACCCCGC
CGCCGGGCTGTTCCTGTCGGCGAGCATGCCGTGCCGCGACGTCGAGTCCT
TACCGAGCCGTTCGTCTCCTGGTGCGTGTCCCGCGACATCGACCCCGCCG
CCGGGCTGTTCCTGTCGGCGAGCATGCCGTGCCGCGACGTCGAGTCTGCC
AAGGGCGTCTTCAGCGGCGCTAGGGGCGGCGGACGGCTTAGCGACGTCGC
GTCAAATCGAGGTGCCAGCGGCATAGACGGTGTGGTGAGCTCGGCGATCG
GCTACGCGGCGGGGCTGGGGGCGCCGGTCACCCTGCTGATCGGTGATATG
GCGACGCTGCACGACCTCGGCTCGCTGCACGCGCTACGGGGCCTGGACGC
GACGTCCCACCCAGTCACGGTGGTCTGCGTGAACAATGGAGGTTGCCAAG
GGCGTCTTCAGCGGCGCTAGGGGCGGCGGACGGCTTAGCGACGTCGCGTC
AAATCGAGGTGCCAGCGGCATAGACGGTGTGGTGAGCTCGGCGATCGGCT
ACGCGGCGGGGCTGGGGGCGCCGGTCACCCTGCTGATCGGTGATATGGCG
ACGCTGCACGACCTCGGCTCGCTGCACGCGCTACGGGGCCTGGACGCGAC
GTCCCACCCAGTCACGGTGGTCTGCGTGAACAATGGAGGT
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