mRNA_M-pyrifera_M_contig89328.20705.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig89328.20705.1
Unique NamemRNA_M-pyrifera_M_contig89328.20705.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Match: A0A7S0K7E7_CAFRO (Hypothetical protein (Fragment) n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A7S0K7E7_CAFRO)

HSP 1 Score: 131 bits (330), Expect = 1.510e-33
Identity = 76/177 (42.94%), Postives = 103/177 (58.19%), Query Frame = 1 
Query:   10 FGALRALRPIFLLERLRNVRKVVTNIMKMVPKVVNVAILLGFANLFFGVTGFLLFTE-----------------------------CYNYFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASLFFVLYLVAALFFLLNLTLAVAADEFKRLTEKKVVVRYENMFLGFDQAFKEL 453
            F  +R  RP+FLLER RNVR++  +I+   PK+++V  +LG AN+ FGV GF+L                                C +YF+SL +SM   F L TTANFP+VMLP +RC    ++ FV Y++ + FFLLNLTLA    EFK LT++KV+ RY N+F G D AF+ L
Sbjct:  219 FSWVRLTRPVFLLERFRNVRRIAASIISATPKILSVGAMLGMANILFGVLGFVLLAGIDGDQCTAFAGNPRPPGGCSAFLSADKGGCSDYFASLFDSMTQFFVLTTTANFPEVMLPAYRCSPGYAVLFVSYVLLSTFFLLNLTLAATYIEFKALTQRKVLARYSNIFDGVDLAFERL 395
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BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Match: A0A5A8DD30_CAFRO (EF-hand domain-containing protein n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DD30_CAFRO)

HSP 1 Score: 131 bits (330), Expect = 1.460e-32
Identity = 76/177 (42.94%), Postives = 103/177 (58.19%), Query Frame = 1 
Query:   10 FGALRALRPIFLLERLRNVRKVVTNIMKMVPKVVNVAILLGFANLFFGVTGFLLFTE-----------------------------CYNYFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASLFFVLYLVAALFFLLNLTLAVAADEFKRLTEKKVVVRYENMFLGFDQAFKEL 453
            F  +R  RP+FLLER RNVR++  +I+   PK+++V  +LG AN+ FGV GF+L                                C +YF+SL +SM   F L TTANFP+VMLP +RC    ++ FV Y++ + FFLLNLTLA    EFK LT++KV+ RY N+F G D AF+ L
Sbjct:  101 FSWVRLTRPVFLLERFRNVRRIAASIISATPKILSVGAMLGMANILFGVLGFVLLAGIDGDQCTAFAGNPRPPGGCSAFLSADKGGCSDYFASLFDSMTQFFVLTTTANFPEVMLPAYRCSPGYAVLFVSYVLLSTFFLLNLTLAATYIEFKALTQRKVLARYSNIFDGVDLAFERL 277
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BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Match: A0A2G8KRI8_STIJA (Two-pore calcium channel 1 (Fragment) n=1 Tax=Stichopus japonicus TaxID=307972 RepID=A0A2G8KRI8_STIJA)

HSP 1 Score: 93.6 bits (231), Expect = 2.350e-19
Identity = 57/160 (35.62%), Postives = 92/160 (57.50%), Query Frame = 1 
Query:    1 EGYFGALRALRPIFLLE--RLRNVRKVVTNIMKMVPKVVNVAILLGFANLFFGVTGFLLFTEC--YNYFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASLFFVLYLVAALFFLLNLTLAVAADEFKRLTEKKVVVRYENMFLGFDQAFKELWKAEH 468
            + +F   RALRP F ++    R VR+ +  I + +P +V + +LL +  L F   GF LF++    +YF ++  S  +LF LQTTAN+PDVM+P +     ++LFF+++L+  L+FL N+ LAV  + F  + ++K    Y +  LG   AF+ L    H
Sbjct:  175 QSHFRVTRALRPFFFIDCHYSRGVRRSLRQIFQSLPPIVEMLVLLFYFLLIFATLGFYLFSKIDHNDYFRTMGASFVNLFVLQTTANYPDVMMPAYNSSRWSALFFIVFLILELYFLTNVLLAVVYNTFTDVEKEKFKRLYLHKRLGASMAFRRLCSRMH 334
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BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Match: A7RM05_NEMVE (Predicted protein n=5 Tax=Nematostella vectensis TaxID=45351 RepID=A7RM05_NEMVE)

HSP 1 Score: 84.3 bits (207), Expect = 4.830e-16
Identity = 51/136 (37.50%), Postives = 81/136 (59.56%), Query Frame = 1 
Query:    1 EGYFGALRALRPIFLLER--LRNVRKVVTNIMKMVPKVVNVAILLGFANLFFGVTGFLLFTECYN--YFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASLFFVLYLVAALFFLLNLTLAVAADEFKRLTEKK 396
            + +    RALRP+FL++   LR VR+ +  +M  +P ++++ +LL F    F + GF LF+   +  YF++   S   LF L TTAN+PDVM+P +     + +FF+ Y+  AL+FL+NL LAV  D F  + + K
Sbjct:  139 KNHVRVTRALRPLFLIDSFYLRGVRRTLRQVMLSLPPILDMLVLLLFFIFIFAMLGFYLFSSNQDDEYFTTFERSFISLFVLLTTANYPDVMMPSYAKSRWSVIFFIAYISVALYFLMNLLLAVVYDTFTSIEKDK 274
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BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Match: A0A7S1CET1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CET1_9STRA)

HSP 1 Score: 79.0 bits (193), Expect = 1.470e-15
Identity = 43/89 (48.31%), Postives = 56/89 (62.92%), Query Frame = 1 
Query:  181 CYNYFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASLFFVLYLVAALFFLLNLTLAVAADEFKRLTEKKVVVRYENMFLGFDQAFK 447
            C +YF +L ++M HLFTL TTAN+PDVM+PV+ C + A+ FFVLYL    FFLLNL LAVA   F+     KV    +       +AF+
Sbjct:   50 CTDYFQTLPQAMLHLFTLLTTANYPDVMMPVYNCSQWAAGFFVLYLTLGEFFLLNLVLAVAYHSFQEHATAKVGATVKQRGRALREAFR 138
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BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Match: UPI00109BCE99 (two pore calcium channel protein 1 n=1 Tax=Exaiptasia diaphana TaxID=2652724 RepID=UPI00109BCE99)

HSP 1 Score: 82.4 bits (202), Expect = 2.310e-15
Identity = 52/136 (38.24%), Postives = 80/136 (58.82%), Query Frame = 1 
Query:    1 EGYFGALRALRPIFLLER--LRNVRKVVTNIMKMVPKVVNVAILLGFANLFFGVTGFLLFT--ECYNYFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASLFFVLYLVAALFFLLNLTLAVAADEFKRLTEKK 396
            + +F   RALRP+FL++   LR  R+V+  I+  +P ++++ +LL F    F + GF LF+      YF +   S   LF L TTAN+PDVM+P +     + L+FV Y+  AL+FL+NL LAV  + F  + + K
Sbjct:  181 KNHFRVSRALRPLFLIDSNYLRGTRRVLRQIVLSLPPILDMLVLLMFFIFIFAILGFYLFSLNPKDEYFETFERSFISLFVLLTTANYPDVMMPSYYKSRWSVLYFVAYIGIALYFLMNLLLAVVYETFTSIEKNK 316
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BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Match: A0A6P8HQP4_ACTTE (two pore calcium channel protein 1-like isoform X1 n=3 Tax=Actinia tenebrosa TaxID=6105 RepID=A0A6P8HQP4_ACTTE)

HSP 1 Score: 80.5 bits (197), Expect = 1.080e-14
Identity = 50/136 (36.76%), Postives = 79/136 (58.09%), Query Frame = 1 
Query:    1 EGYFGALRALRPIFLL--ERLRNVRKVVTNIMKMVPKVVNVAILLGFANLFFGVTGFLLFTECYN--YFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASLFFVLYLVAALFFLLNLTLAVAADEFKRLTEKK 396
            E +    RALRP+FL+  E L+  R+V+  I+  +P+++ + +LL F      + GF LF+      YF++   S   LF L TTAN+PDVM+P +     + +FFV Y+  A++FL+NL LAV  + F  + + K
Sbjct:  181 ENHIRITRALRPLFLIDSEYLKGTRRVLRQILFSLPRILGIVLLLLFFIFISSMLGFYLFSSNTKDEYFATFERSFVSLFVLLTTANYPDVMMPSYAMSRWSVIFFVTYVSIAIYFLMNLLLAVVYEAFTSIEKNK 316
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BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Match: A0A7R8VFT0_TIMDO (Hypothetical protein (Fragment) n=1 Tax=Timema douglasi TaxID=61478 RepID=A0A7R8VFT0_TIMDO)

HSP 1 Score: 77.0 bits (188), Expect = 1.400e-13
Identity = 49/136 (36.03%), Postives = 80/136 (58.82%), Query Frame = 1 
Query:    1 EGYFGALRALRPIFLLERLR--NVRKVVTNIMKMVPKVVNVAILLGFANLFFGVTGFLLFT--ECYNYFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASLFFVLYLVAALFFLLNLTLAVAADEFKRLTEKK 396
              +F   RALRPIFL++      VR+ +  I + +P ++++  LL F    + + G+ LF+      +F++L +S   +F L TTANFPDVM+P++   +  S+FF+ YL   L+ L+NL LAV  + F R+  +K
Sbjct:  174 SSHFRVTRALRPIFLVDTCHCGGVRRFIRQIFQSLPPILDMLGLLMFFISIYALLGYYLFSGEPRTEHFTTLHDSFVSMFVLLTTANFPDVMMPLYAKSKWYSVFFISYLCIVLYILMNLMLAVVYETFTRIEREK 309
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BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Match: A0A7R9E9V8_9NEOP (Hypothetical protein n=2 Tax=Timema TaxID=61471 RepID=A0A7R9E9V8_9NEOP)

HSP 1 Score: 77.0 bits (188), Expect = 1.660e-13
Identity = 49/136 (36.03%), Postives = 80/136 (58.82%), Query Frame = 1 
Query:    1 EGYFGALRALRPIFLLERLR--NVRKVVTNIMKMVPKVVNVAILLGFANLFFGVTGFLLFT--ECYNYFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASLFFVLYLVAALFFLLNLTLAVAADEFKRLTEKK 396
              +F   RALRPIFL++      VR+ +  I + +P ++++  LL F    + + G+ LF+      +F++L +S   +F L TTANFPDVM+P++   +  S+FF+ YL   L+ L+NL LAV  + F R+  +K
Sbjct:  206 SSHFRVTRALRPIFLVDTCHCGGVRRFIRQIFQSLPPILDMLGLLMFFISIYALLGYYLFSGEPRTEHFTTLHDSFVSMFVLLTTANFPDVMMPLYAKSKWYSVFFISYLCIVLYILMNLMLAVVYETFTRIEREK 341
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BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Match: A0A7R9FJF4_9NEOP (Hypothetical protein n=1 Tax=Timema tahoe TaxID=61484 RepID=A0A7R9FJF4_9NEOP)

HSP 1 Score: 77.0 bits (188), Expect = 1.700e-13
Identity = 49/136 (36.03%), Postives = 80/136 (58.82%), Query Frame = 1 
Query:    1 EGYFGALRALRPIFLLERLR--NVRKVVTNIMKMVPKVVNVAILLGFANLFFGVTGFLLFT--ECYNYFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASLFFVLYLVAALFFLLNLTLAVAADEFKRLTEKK 396
              +F   RALRPIFL++      VR+ +  I + +P ++++  LL F    + + G+ LF+      +F++L +S   +F L TTANFPDVM+P++   +  S+FF+ YL   L+ L+NL LAV  + F R+  +K
Sbjct:  120 SSHFRVTRALRPIFLVDTCHCGGVRRFIRQIFQSLPPILDMLGLLMFFISIYALLGYYLFSGEPRTEHFTTLHDSFVSMFVLLTTANFPDVMMPLYAKSKWYSVFFISYLCIVLYILMNLMLAVVYETFTRIEREK 255
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig89328.20705.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S0K7E7_CAFRO1.510e-3342.94Hypothetical protein (Fragment) n=1 Tax=Cafeteria ... [more]
A0A5A8DD30_CAFRO1.460e-3242.94EF-hand domain-containing protein n=3 Tax=Cafeteri... [more]
A0A2G8KRI8_STIJA2.350e-1935.63Two-pore calcium channel 1 (Fragment) n=1 Tax=Stic... [more]
A7RM05_NEMVE4.830e-1637.50Predicted protein n=5 Tax=Nematostella vectensis T... [more]
A0A7S1CET1_9STRA1.470e-1548.31Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]
UPI00109BCE992.310e-1538.24two pore calcium channel protein 1 n=1 Tax=Exaipta... [more]
A0A6P8HQP4_ACTTE1.080e-1436.76two pore calcium channel protein 1-like isoform X1... [more]
A0A7R8VFT0_TIMDO1.400e-1336.03Hypothetical protein (Fragment) n=1 Tax=Timema dou... [more]
A0A7R9E9V8_9NEOP1.660e-1336.03Hypothetical protein n=2 Tax=Timema TaxID=61471 Re... [more]
A0A7R9FJF4_9NEOP1.700e-1336.03Hypothetical protein n=1 Tax=Timema tahoe TaxID=61... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig89328contigM-pyrifera_M_contig89328:11..580 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score95.1
Seed ortholog evalue3e-17
Seed eggNOG ortholog6500.XP_005089220.1
Preferred nameTPCN1
KEGG koko:K16896
KEGG TC1.A.1.11.22,1.A.1.11.25
Hectar predicted targeting categorysignal anchor
GOsGO:0000323,GO:0001508,GO:0003674,GO:0005215,GO:0005216,GO:0005217,GO:0005261,GO:0005262,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005764,GO:0005765,GO:0005768,GO:0005773,GO:0005774,GO:0005886,GO:0006810,GO:0006811,GO:0006812,GO:0006816,GO:0006873,GO:0006874,GO:0006875,GO:0007154,GO:0007165,GO:0007204,GO:0008150,GO:0008324,GO:0009893,GO:0009894,GO:0009896,GO:0009987,GO:0010008,GO:0010506,GO:0010508,GO:0012505,GO:0015075,GO:0015085,GO:0015267,GO:0015276,GO:0015278,GO:0015318,GO:0016020,GO:0019222,GO:0019722,GO:0019725,GO:0019932,GO:0022803,GO:0022834,GO:0022836,GO:0022838,GO:0022839,GO:0022857,GO:0022890,GO:0023052,GO:0030001,GO:0030003,GO:0031090,GO:0031323,GO:0031325,GO:0031329,GO:0031331,GO:0031410,GO:0031982,GO:0032879,GO:0034220,GO:0035556,GO:0042391,GO:0042592,GO:0042802,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044433,GO:0044437,GO:0044440,GO:0044444,GO:0044446,GO:0044464,GO:0046873,GO:0048518,GO:0048519,GO:0048522,GO:0048523,GO:0048878,GO:0050789,GO:0050794,GO:0050801,GO:0050896,GO:0051179,GO:0051209,GO:0051234,GO:0051282,GO:0051283,GO:0051480,GO:0051641,GO:0051649,GO:0051716,GO:0051899,GO:0055065,GO:0055074,GO:0055080,GO:0055082,GO:0055085,GO:0060401,GO:0060402,GO:0065007,GO:0065008,GO:0070588,GO:0070838,GO:0071944,GO:0072345,GO:0072503,GO:0072507,GO:0072511,GO:0086010,GO:0097553,GO:0097708,GO:0098588,GO:0098655,GO:0098660,GO:0098662,GO:0098771,GO:0098805,GO:0098852,GO:0099094,GO:0099604
EggNOG free text desc.NAADP-sensitive calcium-release channel activity
EggNOG OGs38C6H@33154,3B9ZU@33208,3CSBT@33213,KOG2301@1,KOG2301@2759
COG Functional cat.PT
Best tax levelBilateria
Best eggNOG OGNA|NA|NA
BRITEko00000,ko04040
Exons2
Model size468
Cds size468
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816803.409242-CDS-M-pyrifera_M_contig89328:10..1851622816803.409242-CDS-M-pyrifera_M_contig89328:10..185Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89328 11..185 +
1692278187.961691-CDS-M-pyrifera_M_contig89328:10..1851692278187.961691-CDS-M-pyrifera_M_contig89328:10..185Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89328 11..185 +
1622816803.4217253-CDS-M-pyrifera_M_contig89328:287..5801622816803.4217253-CDS-M-pyrifera_M_contig89328:287..580Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89328 288..580 +
1692278187.9767714-CDS-M-pyrifera_M_contig89328:287..5801692278187.9767714-CDS-M-pyrifera_M_contig89328:287..580Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89328 288..580 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig89328.20705.1prot_M-pyrifera_M_contig89328.20705.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig89328 11..580 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig89328.20705.1

>prot_M-pyrifera_M_contig89328.20705.1 ID=prot_M-pyrifera_M_contig89328.20705.1|Name=mRNA_M-pyrifera_M_contig89328.20705.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=156bp
EGYFGALRALRPIFLLERLRNVRKVVTNIMKMVPKVVNVAILLGFANLFF
GVTGFLLFTECYNYFSSLSESMYHLFTLQTTANFPDVMLPVFRCDESASL
FFVLYLVAALFFLLNLTLAVAADEFKRLTEKKVVVRYENMFLGFDQAFKE
LWKAEH
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mRNA from alignment at M-pyrifera_M_contig89328:11..580+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig89328.20705.1 ID=mRNA_M-pyrifera_M_contig89328.20705.1|Name=mRNA_M-pyrifera_M_contig89328.20705.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=570bp|location=Sequence derived from alignment at M-pyrifera_M_contig89328:11..580+ (Macrocystis pyrifera P11B4 male)
GAAGGGTACTTTGGTGCATTGCGGGCGCTTCGGCCTATTTTCCTGTTGGA
ACGACTACGTAATGTGCGTAAGGTGGTAACCAACATTATGAAGATGGTAC
CTAAAGTGGTGAACGTAGCTATTTTGTTGGGTTTTGCCAACCTTTTCTTC
GGTGTGACTGGGTTTTTGCTGTTTAGTGAGCGATATGCGTTTGGAGACGA
AAAGTGTCGGACACACGATTTGCCCAAAAGGGTGCAACTGTGCTCTGTGT
TCACCAAATCTGACATTTACACGGGAGCAGAGTGCTACAACTACTTTTCG
TCCCTTTCCGAATCCATGTACCACCTTTTCACGCTGCAAACGACGGCCAA
CTTTCCCGACGTCATGTTGCCCGTGTTTCGTTGCGATGAAAGTGCGTCAC
TCTTCTTTGTGCTGTACTTGGTGGCAGCTCTCTTCTTCCTGCTCAACTTG
ACCTTGGCTGTGGCAGCTGACGAATTCAAGCGCTTGACGGAGAAGAAGGT
GGTGGTGCGGTATGAAAACATGTTCCTGGGCTTTGATCAAGCCTTCAAGG
AGCTGTGGAAGGCAGAACAT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig89328:11..580+

>mRNA_M-pyrifera_M_contig89328.20705.1 ID=mRNA_M-pyrifera_M_contig89328.20705.1|Name=mRNA_M-pyrifera_M_contig89328.20705.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=936bp|location=Sequence derived from alignment at M-pyrifera_M_contig89328:11..580+ (Macrocystis pyrifera P11B4 male)
GAAGGGTACTTTGGTGCATTGCGGGCGCTTCGGCCTATTTTCCTGTTGGA
ACGACTACGTAATGTGCGTAAGGTGGTAACCAACATTATGAAGATGGTAC
CTAAAGTGGTGAACGTAGCTATTTTGTTGGGTTTTGCCAACCTTTTCTTC
GGTGTGACTGGGTTTTTGCTGTTTAGAAGGGTACTTTGGTGCATTGCGGG
CGCTTCGGCCTATTTTCCTGTTGGAACGACTACGTAATGTGCGTAAGGTG
GTAACCAACATTATGAAGATGGTACCTAAAGTGGTGAACGTAGCTATTTT
GTTGGGTTTTGCCAACCTTTTCTTCGGTGTGACTGGGTTTTTGCTGTTTA
CAGAGTGCTACAACTACTTTTCGTCCCTTTCCGAATCCATGTACCACCTT
TTCACGCTGCAAACGACGGCCAACTTTCCCGACGTCATGTTGCCCGTGTT
TCGTTGCGATGAAAGTGCGTCACTCTTCTTTGTGCTGTACTTGGTGGCAG
CTCTCTTCTTCCTGCTCAACTTGACCTTGGCTGTGGCAGCTGACGAATTC
AAGCGCTTGACGGAGAAGAAGGTGGTGGTGCGGTATGAAAACATGTTCCT
GGGCTTTGATCAAGCCTTCAAGGAGCTGTGGAAGGCAGAACATCAGAGTG
CTACAACTACTTTTCGTCCCTTTCCGAATCCATGTACCACCTTTTCACGC
TGCAAACGACGGCCAACTTTCCCGACGTCATGTTGCCCGTGTTTCGTTGC
GATGAAAGTGCGTCACTCTTCTTTGTGCTGTACTTGGTGGCAGCTCTCTT
CTTCCTGCTCAACTTGACCTTGGCTGTGGCAGCTGACGAATTCAAGCGCT
TGACGGAGAAGAAGGTGGTGGTGCGGTATGAAAACATGTTCCTGGGCTTT
GATCAAGCCTTCAAGGAGCTGTGGAAGGCAGAACAT
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