mRNA_M-pyrifera_M_contig89209.20678.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig89209.20678.1
Unique NamemRNA_M-pyrifera_M_contig89209.20678.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Match: A0A813B7I6_9DINO (Jmjd6 protein (Fragment) n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813B7I6_9DINO)

HSP 1 Score: 194 bits (494), Expect = 1.660e-59
Identity = 92/146 (63.01%), Postives = 105/146 (71.92%), Query Frame = 1
Query:    1 LQKLYEKYRHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSGFAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGTCVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAKGMQKKGEDD 438
             Q LY++ RHRKFKCGEDDDG  +K+KLKY LRY     DDSP+YIFDS F ED R KTLLS + VP YF  D+F  +      RPP+RWFL+GP+RSGT  HTDPL TSAWNALV G K WVLFPP  P+ + KAK  QKK EDD
Sbjct:   44 FQNLYDELRHRKFKCGEDDDGYAVKMKLKYFLRYMEHQRDDSPMYIFDSTFDEDKRAKTLLSSYSVPEYFNEDLFRLV--GESRRPPYRWFLIGPKRSGTSPHTDPLATSAWNALVVGRKRWVLFPPHTPRKYTKAKEFQKKPEDD 187          
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Match: A0A7S3A9P3_9RHOD (Hypothetical protein (Fragment) n=3 Tax=Rhodosorus marinus TaxID=101924 RepID=A0A7S3A9P3_9RHOD)

HSP 1 Score: 192 bits (489), Expect = 5.770e-58
Identity = 86/152 (56.58%), Postives = 112/152 (73.68%), Query Frame = 1
Query:    1 LQKLYEKYRHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSGFAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGTCVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAKGMQKKGEDDEAIDYF 456
            L+ L+ +YRH KF+ G+DDDG  +++K+KY LRY  +  DDSPLYIFDS F +    + L+ D+EVP YF+ D+F  +    H RPP+RWFLVGP+RSG+ VH DPLGTSAWNAL+ G K W+LFPP  PK  VKAK +++ GEDDE +DYF
Sbjct:  100 LKSLHRRYRHVKFRVGDDDDGYAVRIKMKYFLRYMMSQTDDSPLYIFDSRFIKLDGSRILIGDYEVPKYFREDLFHLM--GEHRRPPYRWFLVGPKRSGSGVHIDPLGTSAWNALISGRKRWILFPPNVPKKIVKAKNLKRIGEDDEPVDYF 249          
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Match: A0A482T184_9ARCH (JmjC domain-containing protein (Fragment) n=1 Tax=archaeon TaxID=1906665 RepID=A0A482T184_9ARCH)

HSP 1 Score: 193 bits (491), Expect = 6.480e-58
Identity = 90/152 (59.21%), Postives = 110/152 (72.37%), Query Frame = 1
Query:    1 LQKLYEKYRHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSGFAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGTCVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAKGMQKKGEDDEAIDYF 456
            L++L E Y  R FKCGEDDDG K+K+KLKY L Y   N DDSPLYIFDS F ED   +++++DF++P YF  D+F  +  K   RPP+RWFL+GP RSG+ VH DPLGTSAWN L+ G K WVLF PG  K  VK K + KKGEDDEAI++F
Sbjct:  103 LRQLKEDYGERMFKCGEDDDGYKIKMKLKYFLNYLHNNTDDSPLYIFDSNFYEDSSSRSIMNDFKLPSYFPDDLFSLVGEKK--RPPYRWFLIGPERSGSTVHIDPLGTSAWNTLISGRKRWVLFRPGVAKHIVKGKEVIKKGEDDEAINFF 252          
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Match: A0A5A8DX65_CAFRO (JmjC domain-containing protein n=3 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DX65_CAFRO)

HSP 1 Score: 195 bits (495), Expect = 1.760e-57
Identity = 94/153 (61.44%), Postives = 111/153 (72.55%), Query Frame = 1
Query:    4 QKLYEKYRHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSGFAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGTCVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAK--GMQKKGEDDEAIDYF 456
            ++L ++ RHR FKCGEDDDG+ +K+KLKY  RY AT  DDSPLYIFDS F +D R K L+ DFEVP  F  D+F         RPP+RWFL+GPRRSGTC+H DPL TSAWNALVRG K WVLFPP A    V+ K  G+ + GEDDEA+DYF
Sbjct:  112 ERLSKRLRHRMFKCGEDDDGHAVKIKLKYFNRYMATTRDDSPLYIFDSAFTKDDRCKKLMDDFEVPPQFPEDLFQLSGDSR--RPPYRWFLMGPRRSGTCLHIDPLATSAWNALVRGRKRWVLFPPDADVPLVRGKVDGIVRSGEDDEAVDYF 262          
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Match: A0A1Z5JES6_FISSO (Histone arginine demethylase JMJD6 n=2 Tax=Fistulifera solaris TaxID=1519565 RepID=A0A1Z5JES6_FISSO)

HSP 1 Score: 192 bits (488), Expect = 2.790e-57
Identity = 88/147 (59.86%), Postives = 107/147 (72.79%), Query Frame = 1
Query:   16 EKYRHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSGFAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGTCVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAKGMQKKGEDDEAIDYF 456
            E+ R+R+ KCGEDDDG+K+K++LKY L Y   N DDSPLYIFDS F ED   K +L+D++VP YF+ D+F  +      RPP+RWFLVGP+RSGT VH DPLGTSAWN L+ G K WVLFPP  PK  VK KG+    EDDEA+ YF
Sbjct:  119 EELRNRRLKCGEDDDGHKVKVRLKYFLEYLRNNNDDSPLYIFDSNFDEDKYAKRILTDYQVPSYFRDDLFRFV--SESRRPPYRWFLVGPKRSGTTVHIDPLGTSAWNTLIHGQKRWVLFPPHVPKRIVKGKGLIASNEDDEAVHYF 263          
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Match: A0A8J9SMN6_PHATR (Hypothetical protein (Fragment) n=2 Tax=Phaeodactylum tricornutum TaxID=2850 RepID=A0A8J9SMN6_PHATR)

HSP 1 Score: 184 bits (467), Expect = 4.290e-56
Identity = 84/144 (58.33%), Postives = 104/144 (72.22%), Query Frame = 1
Query:   25 RHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSGFAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGTCVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAKGMQKKGEDDEAIDYF 456
            R R FKCGEDDDG  +K+KLK+ ++Y  +NADDSPLYIFD+ F ED + K +L+D+ VP YF  D+F  +      RPP+RWFLVGP RSG+ VH DPL TSAWN L+ G K WVLFPP  PK  VK +G+ ++ EDDEAI YF
Sbjct:   57 RDRFFKCGEDDDGKSVKVKLKHFIKYLQSNADDSPLYIFDTSFEEDRKAKRVLADYRVPSYFSDDLFQLV--SEARRPPYRWFLVGPERSGSTVHVDPLATSAWNTLMFGKKRWVLFPPQVPKQVVKGRGLVRRDEDDEAIHYF 198          
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Match: A0A6U3QEW5_9STRA (Hypothetical protein n=1 Tax=Ditylum brightwellii TaxID=49249 RepID=A0A6U3QEW5_9STRA)

HSP 1 Score: 191 bits (485), Expect = 7.580e-56
Identity = 90/144 (62.50%), Postives = 103/144 (71.53%), Query Frame = 1
Query:   25 RHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSGFAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGTCVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAKGMQKKGEDDEAIDYF 456
            R R FKCGEDDDG  +++KLK+ LRY     DDSPLYIFDS + ED   K LL+D+ VP YFK D+F  +  +   RPP+RWFLVGP RSGTCVH DPL TSAWN L+ G K WVLFPP  PK  VK KG+ KK EDDEAI YF
Sbjct:  159 RDRSFKCGEDDDGKSIRIKLKHFLRYLHDTRDDSPLYIFDSTYDEDKLAKRLLTDYRVPEYFKEDLFHLVGERR--RPPYRWFLVGPERSGTCVHIDPLATSAWNTLIYGAKRWVLFPPHTPKHVVKGKGLIKKNEDDEAIHYF 300          
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Match: A0A0B1S7D1_OESDE (JmjC domain protein (Fragment) n=1 Tax=Oesophagostomum dentatum TaxID=61180 RepID=A0A0B1S7D1_OESDE)

HSP 1 Score: 179 bits (454), Expect = 2.390e-54
Identity = 84/152 (55.26%), Postives = 106/152 (69.74%), Query Frame = 1
Query:    1 LQKLYEKYRHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSGFAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGTCVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAKGMQKKGEDDEAIDYF 456
             Q++  KYR+++FKCGEDDDG  +KLK+KY   Y A   DDSPLYIFDSGF +  +   LL+DF VP +FK D+F+    K   RPP RWF++GP RSGT +H DPLGTSAWN+L+ GHK WVL PP AP+A VK    +K    DEA+ +F
Sbjct:   15 FQRISRKYRNQRFKCGEDDDGYSVKLKMKYYADYMANTKDDSPLYIFDSGFGDRHKTSKLLADFTVPEFFKDDLFNYADYKK--RPPHRWFVMGPARSGTSMHIDPLGTSAWNSLITGHKRWVLIPPDAPRALVKPYQSEKGKHPDEAVTWF 164          
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Match: D7G461_ECTSI (JMJD6 protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G461_ECTSI)

HSP 1 Score: 186 bits (472), Expect = 2.490e-54
Identity = 90/152 (59.21%), Postives = 106/152 (69.74%), Query Frame = 1
Query:    1 LQKLYEKYRHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSGFAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGTCVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAKGMQKKGEDDEAIDYF 456
            L++LY +Y   K KCGEDDDG  +K+KLKY LRY     DDSPLYIFDS F +    K LL DF VPHYF  D+F  +  +   RPP+RWFLVGP+RSGT VH DPLGTSAWN +V G KLWVLFPP   K   K K + ++GEDDE I+YF
Sbjct:  116 LEQLYRRYGDCKLKCGEDDDGYSVKVKLKYFLRYMDHQTDDSPLYIFDSHFDDHEVAKGLLEDFFVPHYFPDDLFSLVGERR--RPPYRWFLVGPKRSGTSVHIDPLGTSAWNTVVSGRKLWVLFPPHVDKNVAKGKDVIRQGEDDEPINYF 265          
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Match: A0A7S2R8H1_9STRA (Hypothetical protein n=1 Tax=Eucampia antarctica TaxID=49252 RepID=A0A7S2R8H1_9STRA)

HSP 1 Score: 186 bits (473), Expect = 3.190e-54
Identity = 87/147 (59.18%), Postives = 104/147 (70.75%), Query Frame = 1
Query:   16 EKYRHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSGFAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGTCVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAKGMQKKGEDDEAIDYF 456
            E  R+R  KCGEDDDG+ +++KLK+ L+Y   N DDSPLYIFD+ F ED     LL D+ VP YF  D+F  +  K   RPP+RWFL+GP RSGTCVH DPL TSAWN L+ G K WVLFPP  PK  VK KG+  KGEDDEA++YF
Sbjct:  134 EDLRNRPLKCGEDDDGHTIRIKLKHFLKYLHNNRDDSPLYIFDTTFDEDRYAARLLKDYRVPTYFDEDLFHLVGEKR--RPPYRWFLMGPERSGTCVHIDPLATSAWNTLIHGKKRWVLFPPHIPKYIVKGKGLIYKGEDDEAVNYF 278          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig89209.20678.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A813B7I6_9DINO1.660e-5963.01Jmjd6 protein (Fragment) n=1 Tax=Symbiodinium sp. ... [more]
A0A7S3A9P3_9RHOD5.770e-5856.58Hypothetical protein (Fragment) n=3 Tax=Rhodosorus... [more]
A0A482T184_9ARCH6.480e-5859.21JmjC domain-containing protein (Fragment) n=1 Tax=... [more]
A0A5A8DX65_CAFRO1.760e-5761.44JmjC domain-containing protein n=3 Tax=Cafeteria r... [more]
A0A1Z5JES6_FISSO2.790e-5759.86Histone arginine demethylase JMJD6 n=2 Tax=Fistuli... [more]
A0A8J9SMN6_PHATR4.290e-5658.33Hypothetical protein (Fragment) n=2 Tax=Phaeodacty... [more]
A0A6U3QEW5_9STRA7.580e-5662.50Hypothetical protein n=1 Tax=Ditylum brightwellii ... [more]
A0A0B1S7D1_OESDE2.390e-5455.26JmjC domain protein (Fragment) n=1 Tax=Oesophagost... [more]
D7G461_ECTSI2.490e-5459.21JMJD6 protein n=1 Tax=Ectocarpus siliculosus TaxID... [more]
A0A7S2R8H1_9STRA3.190e-5459.18Hypothetical protein n=1 Tax=Eucampia antarctica T... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig89209contigM-pyrifera_M_contig89209:2..776 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score188.7
Seed ortholog evalue2e-45
Seed eggNOG ortholog115417.EPrPW00000017047
KEGG koko:K11323
Hectar predicted targeting categoryother localisation
EggNOG free text desc.JmjC domain, hydroxylase
EggNOG OGs1MC25@121069,KOG2130@1,KOG2130@2759
COG Functional cat.BT
Best tax levelPythiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko01000,ko03036
Exons2
Model size456
Cds size456
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816802.197557-CDS-M-pyrifera_M_contig89209:1..2081622816802.197557-CDS-M-pyrifera_M_contig89209:1..208Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89209 2..208 -
1692278187.287288-CDS-M-pyrifera_M_contig89209:1..2081692278187.287288-CDS-M-pyrifera_M_contig89209:1..208Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89209 2..208 -
1622816802.2092152-CDS-M-pyrifera_M_contig89209:527..7761622816802.2092152-CDS-M-pyrifera_M_contig89209:527..776Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89209 528..776 -
1692278187.3005507-CDS-M-pyrifera_M_contig89209:527..7761692278187.3005507-CDS-M-pyrifera_M_contig89209:527..776Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig89209 528..776 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig89209.20678.1prot_M-pyrifera_M_contig89209.20678.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig89209 2..776 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig89209.20678.1

>prot_M-pyrifera_M_contig89209.20678.1 ID=prot_M-pyrifera_M_contig89209.20678.1|Name=mRNA_M-pyrifera_M_contig89209.20678.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=152bp
LQKLYEKYRHRKFKCGEDDDGNKLKLKLKYMLRYTATNADDSPLYIFDSG
FAEDGRGKTLLSDFEVPHYFKADIFDKIPPKAHTRPPFRWFLVGPRRSGT
CVHTDPLGTSAWNALVRGHKLWVLFPPGAPKAFVKAKGMQKKGEDDEAID
YF
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mRNA from alignment at M-pyrifera_M_contig89209:2..776-

Legend: polypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig89209.20678.1 ID=mRNA_M-pyrifera_M_contig89209.20678.1|Name=mRNA_M-pyrifera_M_contig89209.20678.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=775bp|location=Sequence derived from alignment at M-pyrifera_M_contig89209:2..776- (Macrocystis pyrifera P11B4 male)
TTGCAGAAATTGTACGAAAAGTATCGCCACCGCAAGTTCAAGTGCGGCGA AGATGACGACGGGAACAAGCTGAAGCTGAAGCTCAAGTACATGCTGCGGT ACACTGCCACCAATGCTGACGACTCCCCGCTGTACATCTTCGACAGCGGT TTTGCAGAGGATGGGCGGGGAAAGACGCTGCTCTCAGACTTTGAAGTGCC GCACTACTTCAAAGCCGACATCTTCGACAAAATTCCACCAAAAGCACACG TAAGACGGGGAAAAGAAGGAGGAAGGAGAGGAGAAAAGAAAGGGTGAAGG TTGGAGGGTGGGGTGGGTGTGGTTTGGGCTCTCCTCATCATTGTTCTCCT CTTCTATCTCCATTCTATCTCCATTCTCCTCATTGAAATCTGCTCTCTTC TCTCACCTCTTCTCTCTTCTCTTCGTCACCTCTTATTCTCCTCATTATTG CTTGCTTTTTTTTCTCCTCATTTTTTCTTGCTTTTTTTTCTTTTTTCTTC CTTATATTTCTCCTCATTTCCCCTCCCCACCTCCCTCTCCCCATTCCCCT CTCCCATTCTTTTCCCAGACGCGTCCTCCGTTTCGCTGGTTTCTGGTTGG TCCTCGTCGGAGTGGGACTTGTGTGCACACTGACCCTTTGGGTACTTCTG CGTGGAATGCGTTGGTGCGTGGACACAAGCTGTGGGTGTTGTTCCCTCCA GGAGCTCCCAAAGCCTTTGTGAAGGCCAAAGGGATGCAAAAGAAAGGAGA AGACGATGAAGCAATTGACTACTTT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig89209:2..776-

>mRNA_M-pyrifera_M_contig89209.20678.1 ID=mRNA_M-pyrifera_M_contig89209.20678.1|Name=mRNA_M-pyrifera_M_contig89209.20678.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=912bp|location=Sequence derived from alignment at M-pyrifera_M_contig89209:2..776- (Macrocystis pyrifera P11B4 male)
TTGCAGAAATTGTACGAAAAGTATCGCCACCGCAAGTTCAAGTGCGGCGA
AGATGACGACGGGAACAAGCTGAAGCTGAAGCTCAAGTACATGCTGCGGT
ACACTGCCACCAATGCTGACGACTCCCCGCTGTACATCTTCGACAGCGGT
TTTGCAGAGGATGGGCGGGGAAAGACGCTGCTCTCAGACTTTGAAGTGCC
GCACTACTTCAAAGCCGACATCTTCGACAAAATTCCACCAAAAGCACACT
TGCAGAAATTGTACGAAAAGTATCGCCACCGCAAGTTCAAGTGCGGCGAA
GATGACGACGGGAACAAGCTGAAGCTGAAGCTCAAGTACATGCTGCGGTA
CACTGCCACCAATGCTGACGACTCCCCGCTGTACATCTTCGACAGCGGTT
TTGCAGAGGATGGGCGGGGAAAGACGCTGCTCTCAGACTTTGAAGTGCCG
CACTACTTCAAAGCCGACATCTTCGACAAAATTCCACCAAAAGCACACAC
GCGTCCTCCGTTTCGCTGGTTTCTGGTTGGTCCTCGTCGGAGTGGGACTT
GTGTGCACACTGACCCTTTGGGTACTTCTGCGTGGAATGCGTTGGTGCGT
GGACACAAGCTGTGGGTGTTGTTCCCTCCAGGAGCTCCCAAAGCCTTTGT
GAAGGCCAAAGGGATGCAAAAGAAAGGAGAAGACGATGAAGCAATTGACT
ACTTTACGCGTCCTCCGTTTCGCTGGTTTCTGGTTGGTCCTCGTCGGAGT
GGGACTTGTGTGCACACTGACCCTTTGGGTACTTCTGCGTGGAATGCGTT
GGTGCGTGGACACAAGCTGTGGGTGTTGTTCCCTCCAGGAGCTCCCAAAG
CCTTTGTGAAGGCCAAAGGGATGCAAAAGAAAGGAGAAGACGATGAAGCA
ATTGACTACTTT
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