mRNA_M-pyrifera_M_contig86899.20204.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig86899.20204.1
Unique NamemRNA_M-pyrifera_M_contig86899.20204.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Match: A0A6C0HEB0_9ZZZZ (RNase H type-1 domain-containing protein n=1 Tax=viral metagenome TaxID=1070528 RepID=A0A6C0HEB0_9ZZZZ)

HSP 1 Score: 61.6 bits (148), Expect = 1.070e-8
Identity = 47/136 (34.56%), Postives = 68/136 (50.00%), Query Frame = 1
Query:   13 FDGGSRPVPSQGGSGAVLYLNGIEI-ANDSATSPFFTSNQAEYFAAVLAVELALSHPSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAISLLPLPPVYHHISRNFNKRADEIANAACISEEAAVTIRN 417
            FDG S+  P   G+GAVLY N  EI  N        T+N AEY   +L +  AL   +   L + GDS+++I+ ++     K   L PL+    +         VY HI R +N RADE+ANAA   ++    I++
Sbjct:   34 FDGCSKGNPGPAGAGAVLYYNDREIWYNRKFVGRKETNNYAEYCGLLLGLNEALRE-NIRELKVCGDSQLVIKQMKGEYKLKSENLYPLYTEAKSMEKQFD-KIVYEHIYRQYNSRADELANAALEDQDFVDEIKS 167          
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Match: A0DYB8_PARTE (RNase H type-1 domain-containing protein n=1 Tax=Paramecium tetraurelia TaxID=5888 RepID=A0DYB8_PARTE)

HSP 1 Score: 60.5 bits (145), Expect = 1.670e-7
Identity = 40/132 (30.30%), Postives = 70/132 (53.03%), Query Frame = 1
Query:    1 FSVFFDGGSRPVPSQGGSGAVLYLNGIEIANDSATSPFFTSNQAEYFAAVLAVELALSHPSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAISLLPLPPVYHHISRNFNKRADEIANAACISEE 396
            ++++FDG S+  P   G+G  L+    +I   +      T+N AE+ A  L +   L+    + L  FGDSK+II+ + N+   K+  L  + + + +   L  +   Y HISRN N+ AD++A+AA +  +
Sbjct:  621 YTLYFDGASKSNPGPAGAGVALFDKMQQIKEITQPLGKQTNNVAEFLALFLGIRYTLNL-GINYLECFGDSKLIIDGMNNKINFKQQHLEDIRVAIYDYAQLFKMVR-YTHISRNQNEIADKLASAAALQSK 750          
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Match: A0A6N8VYZ1_9CHLR (Ribonuclease HI family protein n=2 Tax=Chloroflexi bacterium TaxID=2026724 RepID=A0A6N8VYZ1_9CHLR)

HSP 1 Score: 57.4 bits (137), Expect = 1.940e-7
Identity = 49/129 (37.98%), Postives = 67/129 (51.94%), Query Frame = 1
Query:    7 VFFDGGSRPVPSQGGSGAVLY-LNGIEIANDSATSPF--FTSNQAEYFAAVLAVELALSHPSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAISLLP-LPPVYHHISRNFNKRADEIANAA 381
             + DG SR  P     GAVL   +G+E+  D+  +P    T+N AEY  A+ AVE AL    ++ L +  DS++I+  L+ R   K   L PL   L   I  L    P   H+ RNFN+RAD +AN A
Sbjct:    5 AYADGASRGNPGPAAYGAVLCDADGVEL--DAWGNPIGPATNNVAEYHGAIAAVEAALDR-GATDLELRLDSELIVRQLEGRYRVKNQQLKPLFAQLSALIERLDSFQPT--HVRRNFNQRADAMANEA 128          
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Match: A0A7S1HED2_HEMAN (Hypothetical protein (Fragment) n=1 Tax=Hemiselmis andersenii TaxID=464988 RepID=A0A7S1HED2_HEMAN)

HSP 1 Score: 58.2 bits (139), Expect = 1.980e-7
Identity = 44/127 (34.65%), Postives = 59/127 (46.46%), Query Frame = 1
Query:    7 VFFDGGSRPVPSQGGSGAVLYLNGI--EIANDSATSPFFTSNQAEYFAAVLAVELALSHPSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAISLLPLPPVYHHISRNFNKRADEIANAA 381
            ++FDGGSR  P   GSGAV+YL G   E+   S       +N    +  +L+   A        L + GDSK++I  +QN     K  LLP H      +      P   H+ R  N RAD +ANAA
Sbjct:   27 LYFDGGSRGNPGLAGSGAVIYLAGTTEEVWTGSVFVGICETNNVAEYRGLLSGLSAAEKIRGKSLMVKGDSKLVINQVQNIWRVNKEHLLP-HCNDAKKLLGKIANPKLEHVRREHNTRADALANAA 152          
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Match: UPI0019601160 (reverse transcriptase-like protein n=1 Tax=Virgibacillus halotolerans TaxID=1071053 RepID=UPI0019601160)

HSP 1 Score: 57.0 bits (136), Expect = 1.050e-6
Identity = 42/131 (32.06%), Postives = 66/131 (50.38%), Query Frame = 1
Query:    4 SVFFDGGSRPVPSQGGSGAVLYLNGIE----IANDSATSPFFTSNQAEYFAAVLAV-ELALSHPSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAISLLPLPPVYHHISRNFNKRADEIANAA 381
            +V+FDGG      + G G V+Y +  E    +  ++      T+N+AEY A  LA+ ELA+      P+ I GDSK++I  L +     +  L      + N I  L + P Y  +SR  N+ AD++A+ A
Sbjct:   75 TVYFDGGFDLKTKKSGLGCVIYYDKNEKSFRLRKNALVEELHTNNEAEYAALHLALQELAILEVHHLPVTIVGDSKVVINQLNDEWPCYEAELSKWMDRIENQIKKLGITPEYEVVSRKENREADQLASQA 205          
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Match: A0A0D2GCW2_9DELT (Ribonuclease H n=1 Tax=Dethiosulfatarculus sandiegensis TaxID=1429043 RepID=A0A0D2GCW2_9DELT)

HSP 1 Score: 55.5 bits (132), Expect = 1.290e-6
Identity = 43/123 (34.96%), Postives = 66/123 (53.66%), Query Frame = 1
Query:   16 DGGSRPVPSQGGSGAVLY-LNGIEIANDSATSPFFTSNQAEYFAAVLAVELALSHPSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAISLLPLPPVYHHISRNFNKRADEIANAA 381
            DG SR  P + G+GAVLY   G E+   +      T+N AEY   +L V+ AL    +S L++  DS+++++ L  +   K  +L PL   +   +  +    + H + R FNKRADE+AN A
Sbjct:   19 DGASRGNPGEAGAGAVLYDKEGGEVRALTRYLGKVTNNVAEYQGLILGVKAALELEVAS-LSVRLDSELLVKQLNGQYRVKSPLLKPLFDEVKTLLQGIEQVDIIH-VRREFNKRADELANQA 139          
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Match: A0A7S1NH51_9EUGL (Hypothetical protein n=1 Tax=Eutreptiella gymnastica TaxID=73025 RepID=A0A7S1NH51_9EUGL)

HSP 1 Score: 56.2 bits (134), Expect = 3.960e-6
Identity = 50/188 (26.60%), Postives = 75/188 (39.89%), Query Frame = 1
Query:    4 SVFFDGGSRPVPSQGGSGAVLYLNGIEIANDSATSPF--------------FTSNQAEYFAAVLAVELALSH--PSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAISLL--PLPPVYHHISRNFNKRADEIANAACISEEAAVTIRNQPQNGRSNALP--------RAPPSLFSLLRKW 489
            +++FDGGSR  P   G+GA+L     E      +S F               T+N AEY   +L +E  L H       L ++GDSK++++ +  +       L PLH      +  L        HH+ R  N  AD ++N A  S +      + P     N+L         R P S  S+   W
Sbjct:   41 ALWFDGGSRGNPGTAGAGAILRATCSEPLGREPSSQFNTRVLWKGYTFIGKHTNNVAEYKGLLLGLETVLLHLVDQCKRLEVYGDSKLVVQQVTGKWRVHNEGLKPLHERAQALLQRLHKQCRVSVHHVRRQHNTEADALSNVAMDSRQDWEEWTDPPSQSYPNSLDLTSGPTPIRVPASTSSITPTW 228          
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Match: A0A329STI0_9STRA (RNase H type-1 domain-containing protein n=1 Tax=Phytophthora cactorum TaxID=29920 RepID=A0A329STI0_9STRA)

HSP 1 Score: 55.5 bits (132), Expect = 8.260e-6
Identity = 47/135 (34.81%), Postives = 63/135 (46.67%), Query Frame = 1
Query:    1 FSVFFDGGSRPVPSQGGSGAVLYLNG-----IEI---ANDSATSPFFTSNQAEYFAAVLAVELALSHPSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAISLLPLPPVYHHISRNFNKRADEIANAA 381
            + + FDGGSR  P  GGSGAV+   G     +++   A  S T    T+N AE    +  +   + + + SPLNI GDS++II     R  P    L  +          L L    HH  R FNK AD +AN A
Sbjct:  375 YLLLFDGGSRGNPGPGGSGAVVVDLGQTATQVKVCWFAGMSYTDKATTNNDAENMGLLSGLRACVKY-NWSPLNIVGDSQLIIRQRTRRKAPLARYLPTIFWKCRRLTGKLKLLTWQHH-RREFNKAADALANVA 507          
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Match: A0A2W5XGG1_9BACT (Ribonuclease H n=2 Tax=Terrabacteria group TaxID=1783272 RepID=A0A2W5XGG1_9BACT)

HSP 1 Score: 53.5 bits (127), Expect = 9.240e-6
Identity = 42/129 (32.56%), Postives = 64/129 (49.61%), Query Frame = 1
Query:    1 FSVFFDGGSRPVPSQGGSGAVLY-LNGIEIANDSATSPFFTSNQAEYFAAVLAVELALSHPSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAISLLPLPPVYH--HISRNFNKRADEIANA 378
            ++++ DGGSR  P    +G V+   +G  +A         T+N AEY A +L ++ A       P+ +  DS++++  L      K   ++PLH     A  LL      H  H++RN NKRADEI NA
Sbjct:   29 YTIYTDGGSRGNPGPAAAGGVIVGADGSLLAELGEYLGVATNNVAEYRALILMLQKA-RELGCDPIEVRMDSELVVRQLNGSYRVKDAKMIPLH---AQATRLLRGFSASHIRHVTRNENKRADEIVNA 153          
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Match: A0A536MWI7_9CHLR (Ribonuclease HI family protein n=2 Tax=Chloroflexi bacterium TaxID=2026724 RepID=A0A536MWI7_9CHLR)

HSP 1 Score: 52.8 bits (125), Expect = 1.250e-5
Identity = 43/128 (33.59%), Postives = 62/128 (48.44%), Query Frame = 1
Query:    7 VFFDGGSRPVPSQGGSGAVLY-LNGIEIANDSATSPFFTSNQAEYFAAVLAVELALSHPSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAISLLPLPPVYH--HISRNFNKRADEIANAA 381
            +F DG SR  P   G G V+   NG+ +          T+N+AEY A +  +  A+       L ++ DSK+++E +  R   K   L+PLH     AI LL   P  H  H+ R  N+ AD +AN A
Sbjct:    6 LFTDGASRGNPGPAGIGVVIEDENGMRLRGLHRWLGVTTNNEAEYHALIEGLR-AVKDWKPDRLEVYLDSKLVVEQMNGRYRIKAPELIPLH---EKAIGLLRQFPDVHVAHVDREKNRGADALANMA 129          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig86899.20204.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 15
Match NameE-valueIdentityDescription
A0A6C0HEB0_9ZZZZ1.070e-834.56RNase H type-1 domain-containing protein n=1 Tax=v... [more]
A0DYB8_PARTE1.670e-730.30RNase H type-1 domain-containing protein n=1 Tax=P... [more]
A0A6N8VYZ1_9CHLR1.940e-737.98Ribonuclease HI family protein n=2 Tax=Chloroflexi... [more]
A0A7S1HED2_HEMAN1.980e-734.65Hypothetical protein (Fragment) n=1 Tax=Hemiselmis... [more]
UPI00196011601.050e-632.06reverse transcriptase-like protein n=1 Tax=Virgiba... [more]
A0A0D2GCW2_9DELT1.290e-634.96Ribonuclease H n=1 Tax=Dethiosulfatarculus sandieg... [more]
A0A7S1NH51_9EUGL3.960e-626.60Hypothetical protein n=1 Tax=Eutreptiella gymnasti... [more]
A0A329STI0_9STRA8.260e-634.81RNase H type-1 domain-containing protein n=1 Tax=P... [more]
A0A2W5XGG1_9BACT9.240e-632.56Ribonuclease H n=2 Tax=Terrabacteria group TaxID=1... [more]
A0A536MWI7_9CHLR1.250e-533.59Ribonuclease HI family protein n=2 Tax=Chloroflexi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig86899contigM-pyrifera_M_contig86899:44..571 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score63.5
Seed ortholog evalue1.1e-07
Seed eggNOG ortholog2045.KR76_17025
Preferred namernhA
KEGG rclassRC00017,RC00536
KEGG koko:K02226,ko:K03469,ko:K06864,ko:K15634,ko:K22305,ko:K22306,ko:K22316
KEGG ReactionR00582,R01518,R04594,R11173
KEGG Pathwayko00010,ko00260,ko00680,ko00860,ko01100,ko01110,ko01120,ko01130,ko01200,ko01230,ko03030,map00010,map00260,map00680,map00860,map01100,map01110,map01120,map01130,map01200,map01230,map03030
KEGG ModuleM00001,M00002,M00003,M00122
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003676,GO:0003824,GO:0004518,GO:0004519,GO:0004521,GO:0004523,GO:0004540,GO:0005488,GO:0006139,GO:0006401,GO:0006725,GO:0006766,GO:0006767,GO:0006793,GO:0006796,GO:0006807,GO:0008150,GO:0008152,GO:0009056,GO:0009057,GO:0009058,GO:0009110,GO:0009235,GO:0009236,GO:0009987,GO:0016070,GO:0016311,GO:0016787,GO:0016788,GO:0016791,GO:0016891,GO:0016893,GO:0017144,GO:0018130,GO:0019438,GO:0019439,GO:0032296,GO:0033013,GO:0033014,GO:0034641,GO:0034655,GO:0042364,GO:0042578,GO:0043170,GO:0043755,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044260,GO:0044265,GO:0044270,GO:0044271,GO:0044281,GO:0044283,GO:0046483,GO:0046700,GO:0051186,GO:0051188,GO:0071667,GO:0071704,GO:0090304,GO:0090305,GO:0090501,GO:0090502,GO:0097159,GO:0140098,GO:1901360,GO:1901361,GO:1901362,GO:1901363,GO:1901564,GO:1901566,GO:1901575,GO:1901576
EggNOG free text desc.Phosphoglycerate mutase family
EggNOG OGs2GJ9R@201174,4DNYR@85009,COG0328@1,COG0328@2,COG0406@1,COG0406@2
EC3.1.26.4,3.1.3.3,3.1.3.73,3.1.3.85,5.4.2.12
COG Functional cat.GL
Best tax levelPropionibacteriales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000,ko03032
Exons1
Model size528
Cds size528
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816778.002923-CDS-M-pyrifera_M_contig86899:43..5711622816778.002923-CDS-M-pyrifera_M_contig86899:43..571Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig86899 44..571 +
1692278172.810703-CDS-M-pyrifera_M_contig86899:43..5711692278172.810703-CDS-M-pyrifera_M_contig86899:43..571Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig86899 44..571 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig86899.20204.1prot_M-pyrifera_M_contig86899.20204.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig86899 44..571 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig86899.20204.1

>prot_M-pyrifera_M_contig86899.20204.1 ID=prot_M-pyrifera_M_contig86899.20204.1|Name=mRNA_M-pyrifera_M_contig86899.20204.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=176bp
FSVFFDGGSRPVPSQGGSGAVLYLNGIEIANDSATSPFFTSNQAEYFAAV
LAVELALSHPSSSPLNIFGDSKIIIEALQNRTFPKKNILLPLHIMLINAI
SLLPLPPVYHHISRNFNKRADEIANAACISEEAAVTIRNQPQNGRSNALP
RAPPSLFSLLRKWLHPSCYDPSFPLP
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mRNA from alignment at M-pyrifera_M_contig86899:44..571+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig86899.20204.1 ID=mRNA_M-pyrifera_M_contig86899.20204.1|Name=mRNA_M-pyrifera_M_contig86899.20204.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=528bp|location=Sequence derived from alignment at M-pyrifera_M_contig86899:44..571+ (Macrocystis pyrifera P11B4 male)
TTTTCGGTCTTTTTTGATGGAGGTTCTCGCCCTGTCCCCTCTCAAGGGGG ATCTGGTGCAGTCCTCTATCTCAATGGTATTGAAATTGCAAATGACTCAG CTACCTCCCCTTTCTTCACCTCGAACCAGGCGGAGTACTTTGCTGCTGTC CTTGCTGTGGAGCTTGCTCTCTCTCATCCCTCCTCCTCTCCTCTTAATAT CTTTGGTGATTCCAAAATTATTATTGAGGCTCTTCAAAATCGTACCTTCC CCAAAAAGAACATCCTTCTTCCTTTGCACATCATGCTCATTAATGCTATT TCTCTCCTCCCCCTCCCCCCTGTCTATCACCATATCTCAAGGAACTTCAA CAAAAGAGCTGATGAGATTGCCAATGCTGCCTGCATCTCGGAGGAGGCTG CTGTCACCATCCGGAATCAACCCCAGAATGGCCGCAGCAACGCCCTTCCC CGTGCACCTCCCTCTCTTTTCTCTCTCCTCCGAAAATGGCTTCATCCCTC TTGTTATGACCCCTCTTTCCCTCTTCCT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig86899:44..571+

>mRNA_M-pyrifera_M_contig86899.20204.1 ID=mRNA_M-pyrifera_M_contig86899.20204.1|Name=mRNA_M-pyrifera_M_contig86899.20204.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1056bp|location=Sequence derived from alignment at M-pyrifera_M_contig86899:44..571+ (Macrocystis pyrifera P11B4 male)
TTTTCGGTCTTTTTTGATGGAGGTTCTCGCCCTGTCCCCTCTCAAGGGGG
ATCTGGTGCAGTCCTCTATCTCAATGGTATTGAAATTGCAAATGACTCAG
CTACCTCCCCTTTCTTCACCTCGAACCAGGCGGAGTACTTTGCTGCTGTC
CTTGCTGTGGAGCTTGCTCTCTCTCATCCCTCCTCCTCTCCTCTTAATAT
CTTTGGTGATTCCAAAATTATTATTGAGGCTCTTCAAAATCGTACCTTCC
CCAAAAAGAACATCCTTCTTCCTTTGCACATCATGCTCATTAATGCTATT
TCTCTCCTCCCCCTCCCCCCTGTCTATCACCATATCTCAAGGAACTTCAA
CAAAAGAGCTGATGAGATTGCCAATGCTGCCTGCATCTCGGAGGAGGCTG
CTGTCACCATCCGGAATCAACCCCAGAATGGCCGCAGCAACGCCCTTCCC
CGTGCACCTCCCTCTCTTTTCTCTCTCCTCCGAAAATGGCTTCATCCCTC
TTGTTATGACCCCTCTTTCCCTCTTCCTTTTTCGGTCTTTTTTGATGGAG
GTTCTCGCCCTGTCCCCTCTCAAGGGGGATCTGGTGCAGTCCTCTATCTC
AATGGTATTGAAATTGCAAATGACTCAGCTACCTCCCCTTTCTTCACCTC
GAACCAGGCGGAGTACTTTGCTGCTGTCCTTGCTGTGGAGCTTGCTCTCT
CTCATCCCTCCTCCTCTCCTCTTAATATCTTTGGTGATTCCAAAATTATT
ATTGAGGCTCTTCAAAATCGTACCTTCCCCAAAAAGAACATCCTTCTTCC
TTTGCACATCATGCTCATTAATGCTATTTCTCTCCTCCCCCTCCCCCCTG
TCTATCACCATATCTCAAGGAACTTCAACAAAAGAGCTGATGAGATTGCC
AATGCTGCCTGCATCTCGGAGGAGGCTGCTGTCACCATCCGGAATCAACC
CCAGAATGGCCGCAGCAACGCCCTTCCCCGTGCACCTCCCTCTCTTTTCT
CTCTCCTCCGAAAATGGCTTCATCCCTCTTGTTATGACCCCTCTTTCCCT
CTTCCT
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