mRNA_M-pyrifera_M_contig86830.20193.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig86830.20193.1
Unique NamemRNA_M-pyrifera_M_contig86830.20193.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Match: A0A813A1I1_9DINO (NHX7 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813A1I1_9DINO)

HSP 1 Score: 201 bits (511), Expect = 1.170e-60
Identity = 104/169 (61.54%), Postives = 128/169 (75.74%), Query Frame = 1
Query:    1 FAFVYSFSWEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLWNDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVWWLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASSGRTKVREHESMHHFWEMIE 507
            +A  + + W   +AFGAMLSATDPVAVVALL+E+GAS +LSLLIEGESL+NDGTAMV+FIV+ DAM GQ R+ GETVAFFAQLS GGVALG+A G   V  L    +D+V QI  T+F  Y  F L+E T +H+SG+LA V  GL +A+ GRT V EHE++H FWEM+E
Sbjct:    8 YAMPFGWGWNEGLAFGAMLSATDPVAVVALLRELGASPRLSLLIEGESLFNDGTAMVLFIVFADAMKGQARDGGETVAFFAQLSLGGVALGVAAGWITVRVLNALHFDIVAQIATTVFAAYGTFVLAEVTPVHVSGVLATVALGLAMAAGGRTAVHEHETLHEFWEMVE 176          
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Match: A0A7S0PFN2_CAFRO (Hypothetical protein (Fragment) n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A7S0PFN2_CAFRO)

HSP 1 Score: 196 bits (498), Expect = 1.450e-55
Identity = 96/161 (59.63%), Postives = 122/161 (75.78%), Query Frame = 1
Query:   25 WEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLWNDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVWWLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASSGRTKVREHESMHHFWEMIE 507
            W   +AFGAMLSATDPVAVVALLK++GA K+LSLLIEGESL+NDGTAM VF+V+ D M G+ + +GE V F  QL+ GG+ LGL  G  +  W+G  +WD  TQ+TITLF  YL+F  +EG  L +SG+LAVV  GLV+A +GRT +++H  MH+FWEMIE
Sbjct:  167 WATSLAFGAMLSATDPVAVVALLKDLGAPKQLSLLIEGESLFNDGTAMAVFLVFLDLMRGEAKTIGEMVLFLLQLAGGGILLGLGGGIVVATWMGIAKWDATTQVTITLFASYLLFIFAEGA-LEVSGVLAVVGLGLVLAVAGRTSIKDHAMMHYFWEMIE 326          
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Match: A0A812ZJL6_9DINO (NHX7 protein (Fragment) n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812ZJL6_9DINO)

HSP 1 Score: 194 bits (494), Expect = 7.350e-55
Identity = 94/162 (58.02%), Postives = 126/162 (77.78%), Query Frame = 1
Query:   22 SWEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLWNDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVWWLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASSGRTKVREHESMHHFWEMIE 507
            +W A MAFGAMLSATDPVAVVALLKE+GA ++L+L IEGESL+NDGTA  +F+++  AM G+   V E VAFF Q++ GG A+GLA G  +  WL  +RWD +TQ+ +T+   Y  F ++EG  +H+SG+LAVVV GL++A+SGRT +R+HE+MH+FWEMIE
Sbjct:    5 TWNAGMAFGAMLSATDPVAVVALLKELGAPEQLALGIEGESLFNDGTAAALFLLFAGAMRGEVSSVEEQVAFFFQIALGGSAIGLAVGLLVAAWLVWQRWDPITQVAVTIIAAYTTFIVAEGM-MHVSGVLAVVVLGLILAASGRTSIRDHEAMHYFWEMIE 165          
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Match: A0A5A8C0V6_CAFRO (Cyclic nucleotide-binding domain-containing protein n=4 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8C0V6_CAFRO)

HSP 1 Score: 194 bits (494), Expect = 2.240e-54
Identity = 94/162 (58.02%), Postives = 126/162 (77.78%), Query Frame = 1
Query:   22 SWEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLWNDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVWWLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASSGRTKVREHESMHHFWEMIE 507
            +W A MAFGAMLSATDPVAVVALLKE+GA ++L+L IEGESL+NDGTA  +F+++  AM G+   V E VAFF Q++ GG A+GLA G  +  WL  +RWD +TQ+ +T+   Y  F ++EG  +H+SG+LAVVV GL++A+SGRT +R+HE+MH+FWEMIE
Sbjct:  159 TWNAGMAFGAMLSATDPVAVVALLKELGAPEQLALGIEGESLFNDGTAAALFLLFAGAMRGEVSSVEEQVAFFFQIALGGSAIGLAVGLLVAAWLVWQRWDPITQVAVTIIAAYTTFIVAEGM-MHVSGVLAVVVLGLILAASGRTSIRDHEAMHYFWEMIE 319          
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Match: A0A5A8CE95_CAFRO (Cyclic nucleotide-binding domain-containing protein n=4 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CE95_CAFRO)

HSP 1 Score: 194 bits (494), Expect = 2.280e-54
Identity = 95/161 (59.01%), Postives = 122/161 (75.78%), Query Frame = 1
Query:   25 WEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLWNDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVWWLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASSGRTKVREHESMHHFWEMIE 507
            W   +AFGA+LSATDPVAVVALLK++GA K+LSLLIEGESL+NDGTAM VF+V+ D M G+ + +GE V F  QL+ GG+ LGL  G  +  W+G  +WD  TQ+TITLF  YL+F  +EG  L +SG+LAVV  GLV+A +GRT +++H  MH+FWEMIE
Sbjct:  162 WATSLAFGAVLSATDPVAVVALLKDLGAPKQLSLLIEGESLFNDGTAMAVFLVFLDLMRGEAKTIGEMVLFLLQLAGGGILLGLGGGIVVATWMGIAKWDATTQVTITLFASYLLFIFAEGA-LEVSGVLAVVGLGLVLAVAGRTSIKDHAMMHYFWEMIE 321          
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Match: A0A2R6W0F3_MARPO (Cyclic nucleotide-binding domain-containing protein n=5 Tax=Marchantia polymorpha TaxID=3197 RepID=A0A2R6W0F3_MARPO)

HSP 1 Score: 166 bits (421), Expect = 1.440e-44
Identity = 86/168 (51.19%), Postives = 114/168 (67.86%), Query Frame = 1
Query:    7 FVYSFSWEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLWNDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVWWLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASSGRTKV--REHESMHHFWEMI 504
            F Y ++W  C+  G +LSATDPVAVVALLKE+GASKKLS ++EGESL NDGTA+VVF ++   + GQ    G+ V FF+Q++ G VALGLA G   V W+GR   DV+ +IT+TL   YL FF++E    H+SG+L V+  G+  A+  RT       +SM HFWEM+
Sbjct:  188 FPYGWNWSTCLLLGGLLSATDPVAVVALLKELGASKKLSTIVEGESLMNDGTAIVVFKLFLQMVLGQHFTAGDIVQFFSQVALGAVALGLAFGLVTVLWIGRVFNDVIVEITLTLTASYLAFFVAEMEA-HVSGVLTVMTVGMFFAAFARTAFTGESQQSMRHFWEMV 354          
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Match: UPI001C4C3E67 (sodium/hydrogen exchanger 8-like n=1 Tax=Zingiber officinale TaxID=94328 RepID=UPI001C4C3E67)

HSP 1 Score: 158 bits (399), Expect = 2.400e-44
Identity = 84/168 (50.00%), Postives = 115/168 (68.45%), Query Frame = 1
Query:    7 FVYSFSWEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLWNDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVWWLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASSGRT--KVREHESMHHFWEMI 504
            F Y + W+  +  G +LSATDPVAVVALLKE+GASKK++ +IEGESL NDGTA+VVF ++   + GQ+  VGE + F +++S G VA+GLA G A V WLG    D V +IT+TL   Y+ FF ++  G ++SG+LAV+  G+  A+  RT  K     S+HHFWEM+
Sbjct:  135 FPYGWDWKISLLLGGLLSATDPVAVVALLKELGASKKMNTIIEGESLMNDGTAIVVFQLFYQIVMGQSFNVGEIIEFLSKVSLGAVAMGLAFGIASVIWLGFIFNDTVIEITLTLAVSYIAFFTAQD-GANISGVLAVMTLGMFFAAFARTAFKSDSERSLHHFWEMV 301          
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Match: A0A2R6WRW4_MARPO (Cyclic nucleotide-binding domain-containing protein n=1 Tax=Marchantia polymorpha TaxID=3197 RepID=A0A2R6WRW4_MARPO)

HSP 1 Score: 163 bits (413), Expect = 1.710e-43
Identity = 84/170 (49.41%), Postives = 114/170 (67.06%), Query Frame = 1
Query:    1 FAFVYSFSWEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLWNDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVWWLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASSGRTKV--REHESMHHFWEMI 504
            F F Y ++W  C+  G +LSATDPVAVVALLKE+GASKKLS ++EGESL NDGTA+VVF ++   + GQ    G+ + FF+Q++ G VALGLA G   V W+GR   D + +IT+TL   Y+ FF++E     +SG+L V+  G+  A+  RT       +SMHHFWEM+
Sbjct:  139 FLFPYGWNWSTCLLLGGLLSATDPVAVVALLKELGASKKLSTIVEGESLMNDGTAIVVFKLFFQMVLGQHFTPGDIIRFFSQVALGAVALGLAFGLVTVLWIGRVFNDAIVEITLTLTASYMAFFVAEMEA-KVSGVLTVMTVGMFFAAFARTAFTGESQQSMHHFWEMV 307          
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Match: S8C1G0_9LAMI (Salt overly sensitive 1 (Fragment) n=1 Tax=Genlisea aurea TaxID=192259 RepID=S8C1G0_9LAMI)

HSP 1 Score: 155 bits (391), Expect = 1.390e-42
Identity = 86/169 (50.89%), Postives = 114/169 (67.46%), Query Frame = 1
Query:    4 AFVYSFSWEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLWNDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVWWLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASSGRT--KVREHESMHHFWEMI 504
            AF Y ++W+  +  G +LSATDPVAVVALLKE+GASKKLS +IEGESL NDGTA+VV+ ++   + G    +G  V F  Q+S G VA+GLA G A V WLG    D V +IT+TL   Y+ FF S+  GL +SG+LAV+  G+  ++  RT  K    +S+HHFWEM+
Sbjct:  133 AFPYDWNWKTSLLLGGLLSATDPVAVVALLKELGASKKLSTIIEGESLMNDGTAIVVYQLFYRMVNGWRFNLGTLVKFLLQVSLGAVAIGLAFGIASVLWLGFIFNDTVIEITLTLAVSYIAFFTSQ-EGLDVSGVLAVMTLGMFYSAFARTAFKGESQQSLHHFWEMV 300          
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Match: A0A1Y1IAW3_KLENI (Na+/H+ antiporter n=1 Tax=Klebsormidium nitens TaxID=105231 RepID=A0A1Y1IAW3_KLENI)

HSP 1 Score: 158 bits (400), Expect = 9.330e-42
Identity = 85/170 (50.00%), Postives = 115/170 (67.65%), Query Frame = 1
Query:    1 FAFVYSFSWEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLWNDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVWWLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASSGRTKVR-EHE-SMHHFWEMI 504
            F F YS+SW   +  G +LSATDPVAVVALLKE+GASKKL+ +IEGESL NDGTA+VVF ++   +FG       T+ +F Q+SFGGVA+G+A G   + WLG    D + +ITIT+   Y+V+F SE     +SG++ V+  G+  A+ G T    EH+ S+HHFWEM+
Sbjct:   22 FCFPYSWSWGLSLTLGGLLSATDPVAVVALLKELGASKKLNTIIEGESLINDGTAIVVFRLFYRIVFGSKFNFVNTIVYFMQISFGGVAMGIAFGLVAIMWLGFVFNDTLIEITITVSAAYMVYFTSEQNA-GVSGVIGVMFLGIFFAAVGNTAFTGEHKKSLHHFWEMV 190          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig86830.20193.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A813A1I1_9DINO1.170e-6061.54NHX7 protein n=1 Tax=Symbiodinium sp. KB8 TaxID=23... [more]
A0A7S0PFN2_CAFRO1.450e-5559.63Hypothetical protein (Fragment) n=1 Tax=Cafeteria ... [more]
A0A812ZJL6_9DINO7.350e-5558.02NHX7 protein (Fragment) n=1 Tax=Symbiodinium sp. K... [more]
A0A5A8C0V6_CAFRO2.240e-5458.02Cyclic nucleotide-binding domain-containing protei... [more]
A0A5A8CE95_CAFRO2.280e-5459.01Cyclic nucleotide-binding domain-containing protei... [more]
A0A2R6W0F3_MARPO1.440e-4451.19Cyclic nucleotide-binding domain-containing protei... [more]
UPI001C4C3E672.400e-4450.00sodium/hydrogen exchanger 8-like n=1 Tax=Zingiber ... [more]
A0A2R6WRW4_MARPO1.710e-4349.41Cyclic nucleotide-binding domain-containing protei... [more]
S8C1G0_9LAMI1.390e-4250.89Salt overly sensitive 1 (Fragment) n=1 Tax=Genlise... [more]
A0A1Y1IAW3_KLENI9.330e-4250.00Na+/H+ antiporter n=1 Tax=Klebsormidium nitens Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig86830contigM-pyrifera_M_contig86830:323..832 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score158.3
Seed ortholog evalue3.2e-36
Seed eggNOG ortholog42345.XP_008798100.1
Preferred nameSOS1
Hectar predicted targeting categorysignal peptide
GOsGO:0000302,GO:0003674,GO:0005215,GO:0005451,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005886,GO:0006810,GO:0006811,GO:0006812,GO:0006813,GO:0006814,GO:0006873,GO:0006885,GO:0006950,GO:0006970,GO:0006979,GO:0008150,GO:0008324,GO:0009507,GO:0009526,GO:0009536,GO:0009628,GO:0009636,GO:0009651,GO:0009941,GO:0009987,GO:0010035,GO:0010351,GO:0010352,GO:0015075,GO:0015077,GO:0015078,GO:0015079,GO:0015081,GO:0015291,GO:0015297,GO:0015298,GO:0015299,GO:0015318,GO:0015385,GO:0015386,GO:0015491,GO:0015672,GO:0016020,GO:0019222,GO:0019725,GO:0022804,GO:0022821,GO:0022857,GO:0022890,GO:0030001,GO:0030003,GO:0030004,GO:0030641,GO:0031323,GO:0031967,GO:0031975,GO:0034220,GO:0035725,GO:0042221,GO:0042493,GO:0042542,GO:0042592,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044422,GO:0044424,GO:0044434,GO:0044435,GO:0044444,GO:0044446,GO:0044464,GO:0046677,GO:0046873,GO:0048878,GO:0050789,GO:0050794,GO:0050801,GO:0050896,GO:0051179,GO:0051234,GO:0051453,GO:0055067,GO:0055080,GO:0055082,GO:0055085,GO:0065007,GO:0065008,GO:0071804,GO:0071805,GO:0071944,GO:0098655,GO:0098657,GO:0098659,GO:0098660,GO:0098662,GO:0098719,GO:0098739,GO:0098771,GO:0099516,GO:0099587,GO:0140115,GO:1901700,GO:1902600,GO:2000377
EggNOG free text desc.Sodium hydrogen exchanger
EggNOG OGs37RQ7@33090,3G72M@35493,3KV7Z@4447,COG0025@1,KOG1965@2759
COG Functional cat.P
Best tax levelLiliopsida
Best eggNOG OGNA|NA|NA
Exons1
Model size510
Cds size510
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816777.6098907-CDS-M-pyrifera_M_contig86830:322..8321622816777.6098907-CDS-M-pyrifera_M_contig86830:322..832Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig86830 323..832 -
1692278172.5775785-CDS-M-pyrifera_M_contig86830:322..8321692278172.5775785-CDS-M-pyrifera_M_contig86830:322..832Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig86830 323..832 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig86830.20193.1prot_M-pyrifera_M_contig86830.20193.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig86830 323..832 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig86830.20193.1

>prot_M-pyrifera_M_contig86830.20193.1 ID=prot_M-pyrifera_M_contig86830.20193.1|Name=mRNA_M-pyrifera_M_contig86830.20193.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=170bp
FAFVYSFSWEACMAFGAMLSATDPVAVVALLKEMGASKKLSLLIEGESLW
NDGTAMVVFIVYRDAMFGQTREVGETVAFFAQLSFGGVALGLATGAAIVW
WLGRERWDVVTQITITLFGCYLVFFLSEGTGLHLSGILAVVVQGLVIASS
GRTKVREHESMHHFWEMIE*
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mRNA from alignment at M-pyrifera_M_contig86830:323..832-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig86830.20193.1 ID=mRNA_M-pyrifera_M_contig86830.20193.1|Name=mRNA_M-pyrifera_M_contig86830.20193.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=510bp|location=Sequence derived from alignment at M-pyrifera_M_contig86830:323..832- (Macrocystis pyrifera P11B4 male)
TTCGCGTTCGTCTACTCCTTTTCGTGGGAGGCTTGCATGGCGTTCGGGGC GATGCTTTCTGCGACGGATCCGGTTGCGGTGGTGGCCCTGCTGAAGGAGA TGGGCGCGTCGAAGAAGCTGTCGCTGCTGATCGAGGGCGAGAGTCTGTGG AACGACGGGACGGCCATGGTGGTGTTCATCGTGTACAGAGACGCCATGTT CGGGCAGACGAGGGAGGTCGGAGAGACGGTGGCCTTTTTCGCGCAGCTCT CCTTTGGGGGGGTGGCCCTGGGCCTGGCCACGGGCGCTGCCATCGTGTGG TGGCTCGGCAGGGAGCGGTGGGACGTGGTCACCCAGATCACCATCACGCT CTTCGGGTGCTACCTCGTCTTCTTCCTCTCCGAAGGAACCGGACTCCACC TCTCCGGAATCCTCGCAGTCGTCGTGCAGGGCCTCGTCATCGCCTCCTCC GGCAGAACAAAGGTCAGGGAACACGAGTCCATGCACCACTTCTGGGAAAT GATCGAGTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig86830:323..832-

>mRNA_M-pyrifera_M_contig86830.20193.1 ID=mRNA_M-pyrifera_M_contig86830.20193.1|Name=mRNA_M-pyrifera_M_contig86830.20193.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1020bp|location=Sequence derived from alignment at M-pyrifera_M_contig86830:323..832- (Macrocystis pyrifera P11B4 male)
TTCGCGTTCGTCTACTCCTTTTCGTGGGAGGCTTGCATGGCGTTCGGGGC
GATGCTTTCTGCGACGGATCCGGTTGCGGTGGTGGCCCTGCTGAAGGAGA
TGGGCGCGTCGAAGAAGCTGTCGCTGCTGATCGAGGGCGAGAGTCTGTGG
AACGACGGGACGGCCATGGTGGTGTTCATCGTGTACAGAGACGCCATGTT
CGGGCAGACGAGGGAGGTCGGAGAGACGGTGGCCTTTTTCGCGCAGCTCT
CCTTTGGGGGGGTGGCCCTGGGCCTGGCCACGGGCGCTGCCATCGTGTGG
TGGCTCGGCAGGGAGCGGTGGGACGTGGTCACCCAGATCACCATCACGCT
CTTCGGGTGCTACCTCGTCTTCTTCCTCTCCGAAGGAACCGGACTCCACC
TCTCCGGAATCCTCGCAGTCGTCGTGCAGGGCCTCGTCATCGCCTCCTCC
GGCAGAACAAAGGTCAGGGAACACGAGTCCATGCACCACTTCTGGGAAAT
GATCGAGTAATTCGCGTTCGTCTACTCCTTTTCGTGGGAGGCTTGCATGG
CGTTCGGGGCGATGCTTTCTGCGACGGATCCGGTTGCGGTGGTGGCCCTG
CTGAAGGAGATGGGCGCGTCGAAGAAGCTGTCGCTGCTGATCGAGGGCGA
GAGTCTGTGGAACGACGGGACGGCCATGGTGGTGTTCATCGTGTACAGAG
ACGCCATGTTCGGGCAGACGAGGGAGGTCGGAGAGACGGTGGCCTTTTTC
GCGCAGCTCTCCTTTGGGGGGGTGGCCCTGGGCCTGGCCACGGGCGCTGC
CATCGTGTGGTGGCTCGGCAGGGAGCGGTGGGACGTGGTCACCCAGATCA
CCATCACGCTCTTCGGGTGCTACCTCGTCTTCTTCCTCTCCGAAGGAACC
GGACTCCACCTCTCCGGAATCCTCGCAGTCGTCGTGCAGGGCCTCGTCAT
CGCCTCCTCCGGCAGAACAAAGGTCAGGGAACACGAGTCCATGCACCACT
TCTGGGAAATGATCGAGTAA
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