mRNA_M-pyrifera_M_contig86168.20081.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig86168.20081.1
Unique NamemRNA_M-pyrifera_M_contig86168.20081.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Match: A0A813AE61_9DINO (Hypothetical protein n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A813AE61_9DINO)

HSP 1 Score: 107 bits (267), Expect = 2.450e-25
Identity = 60/128 (46.88%), Postives = 81/128 (63.28%), Query Frame = 1
Query:  241 HVLPVNRSGVLRVQEELERRKKRKEEMP-SAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAADILPSTADLGRMEVVGGA--------------VWSRNAGKYFCNELLFRSLHSIRSQ 579
            HVLPVNR+G +   + L +       +P   VLH GLEN A GL++ETV +N  AN SGG   GPAIPGA D+LP+T DLG +++   +              VWSR+AG+Y+CNE+L+RSLH +RS+
Sbjct:    2 HVLPVNRTGAMWTTQYLSQALDSASSLPFDMVLHTGLENQAKGLKLETVASNNLANDSGGASTGPAIPGAPDMLPTTVDLGWVDMASISAAAQFNASLADARQVWSRDAGQYYCNEVLYRSLHLLRSR 129          
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Match: A0A5A8CW37_CAFRO (Threonylcarbamoyl-AMP synthase n=2 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CW37_CAFRO)

HSP 1 Score: 112 bits (280), Expect = 2.760e-25
Identity = 88/245 (35.92%), Postives = 113/245 (46.12%), Query Frame = 1
Query:   85 HHRRAQVLLTGWGPFLNFSTNPSELVARRLARDG-CSVEWDDA-LGSFELCFDVHVLPVNRSGVLRVQEELERRKKRKEEMPSAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAADILPSTADLGRMEVV-----------------------------------------------------------------GGA---------------VWSRNAGKYFCNELLFRSLHSIR 573
            HH    VL TGWGPFLNF+ NPS  VA  L  DG C+ E  +A   SF +C+   +LPVNR+GV+ V+  L+R  +       A+LH+GLEN+A GL++E   AN+EAN SGG   GPA+ GA  I P+TA LGR+ V                                                                  GGA                +SR+AG+YFCNELL+RS H+IR
Sbjct:   47 HH----VLATGWGPFLNFTFNPSMYVA--LHMDGVCTDEVTEAPAASFRVCWTGIMLPVNRTGVMTVENMLKRGVRY-----DAILHMGLENMARGLKLEVAAANYEANDSGGPSRGPAVLGAPFIEPTTAHLGRLAVTANNPGKNPYAAVKAGDHATRDRLLEAAARTSPEALATVRADADAGLLHAWDAAAASSAGAVAGATGGAGAHDTASWPTLNVTDAFSRDAGQYFCNELLYRSAHTIR 280          
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Match: A0A5A8C2M7_CAFRO (Threonylcarbamoyl-AMP synthase n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8C2M7_CAFRO)

HSP 1 Score: 112 bits (280), Expect = 2.870e-25
Identity = 88/245 (35.92%), Postives = 113/245 (46.12%), Query Frame = 1
Query:   85 HHRRAQVLLTGWGPFLNFSTNPSELVARRLARDG-CSVEWDDA-LGSFELCFDVHVLPVNRSGVLRVQEELERRKKRKEEMPSAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAADILPSTADLGRMEVV-----------------------------------------------------------------GGA---------------VWSRNAGKYFCNELLFRSLHSIR 573
            HH    VL TGWGPFLNF+ NPS  VA  L  DG C+ E  +A   SF +C+   +LPVNR+GV+ V+  L+R  +       A+LH+GLEN+A GL++E   AN+EAN SGG   GPA+ GA  I P+TA LGR+ V                                                                  GGA                +SR+AG+YFCNELL+RS H+IR
Sbjct:  459 HH----VLATGWGPFLNFTFNPSMYVA--LHMDGVCTDEVTEAPAASFRVCWTGIMLPVNRTGVMTVENMLKRGVRY-----DAILHMGLENMARGLKLEVAAANYEANDSGGPSRGPAVLGAPFIEPTTAHLGRLAVTANHPGKNPYAAVKAGDHATRDRLLEAAARTSPEALATVRADADAGLLHAWDAAAASSAGAVAGATGGAGAHDTASWPTLNVTDAFSRDAGQYFCNELLYRSAHTIR 692          
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Match: A0A5A8DUV4_CAFRO (Threonylcarbamoyl-AMP synthase n=1 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8DUV4_CAFRO)

HSP 1 Score: 112 bits (280), Expect = 2.880e-25
Identity = 88/245 (35.92%), Postives = 113/245 (46.12%), Query Frame = 1
Query:   85 HHRRAQVLLTGWGPFLNFSTNPSELVARRLARDG-CSVEWDDA-LGSFELCFDVHVLPVNRSGVLRVQEELERRKKRKEEMPSAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAADILPSTADLGRMEVV-----------------------------------------------------------------GGA---------------VWSRNAGKYFCNELLFRSLHSIR 573
            HH    VL TGWGPFLNF+ NPS  VA  L  DG C+ E  +A   SF +C+   +LPVNR+GV+ V+  L+R  +       A+LH+GLEN+A GL++E   AN+EAN SGG   GPA+ GA  I P+TA LGR+ V                                                                  GGA                +SR+AG+YFCNELL+RS H+IR
Sbjct:  330 HH----VLATGWGPFLNFTFNPSMYVA--LHMDGVCTDEVTEAPAASFRVCWTGIMLPVNRTGVMTVENMLKRGVRY-----DAILHMGLENMARGLKLEVAAANYEANDSGGPSRGPAVLGAPFIEPTTAHLGRLAVTANHPGKNPYAAVKAGDHATRDRLLEAAARTSPEALATVRADADAGLLHAWDAAAASSAGAVAGATGGAGAHDTASWPTLNVTDAFSRDAGQYFCNELLYRSAHTIR 563          
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Match: A0A0D2UEL8_CAPO3 (Uncharacterized protein n=1 Tax=Capsaspora owczarzaki (strain ATCC 30864) TaxID=595528 RepID=A0A0D2UEL8_CAPO3)

HSP 1 Score: 100 bits (250), Expect = 2.120e-22
Identity = 67/195 (34.36%), Postives = 102/195 (52.31%), Query Frame = 1
Query:  100 QVLLTGWGPFLNFSTNPSELVARRLARDGCSVEWDDALG-SFELCFDVHVLPVNRSGVLRVQEELER-RKKRKEEMP-SAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAADILPSTADLGRMEV----------VGGAV---------------------WSRNAGKYFCNELLFRSLHSIRSQN 582
            QVLLTG+GPF+N++TNPS+LVA  L         D+ +   + +CF   VLPV  +G + V   L +  +   + +P   ++H+GLE+ A GL  ET+G N  AN S     GP  PG   +LP+T +LG + +          +G  +                     WSR+AG ++CNE L+R+L+ +RS+N
Sbjct:   43 QVLLTGFGPFMNYTTNPSQLVAEALHDQCAQFRADNVVKHDYAVCFRTMVLPVADNGSVAVASYLSKFTEATADRIPYDVIIHMGLEDAAKGLLFETIGFNQRANAS---TPGPIQPGGPTLLPTTVNLGHVRIPELYSLAERRLGRPLATDAALKVNPTRFSANDTTEAWSRDAGTFYCNETLYRTLYQLRSRN 234          
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Match: C5KHG6_PERM5 (Pyrrolidone-carboxylate peptidase, putative n=1 Tax=Perkinsus marinus (strain ATCC 50983 / TXsc) TaxID=423536 RepID=C5KHG6_PERM5)

HSP 1 Score: 91.7 bits (226), Expect = 6.780e-19
Identity = 59/164 (35.98%), Postives = 87/164 (53.05%), Query Frame = 1
Query:   94 RAQVLLTGWGPFLNFSTNPSELVARRLARDGCSVEWDDALGSFELCFDVHVLPVNRSGVLRVQEELERRKKRKEEMPSAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAADILPSTADLGRMEVVGG-AVWSRNAGKYFCNELLFRSLHSIRSQN 582
            R+ V +TG+ PF +F+ NPSE VA+ L    C+  +         C     LPV   G  +    L      K   P+A++HLGLE+ A GL++E    N  A  +   P+   +P    ILPSTA+LG +  +    +WS +AG ++CNE+ FR+L  IRS+N
Sbjct:  104 RSVVWVTGYQPFEDFTFNPSEAVAKSL-NGSCTSHY---------CIQAFSLPVTHGGASQPARWLREADVHK---PAAIVHLGLEDRAKGLKLEVAAKNILAESNSSLPI---LPNVTSILPSTANLGLVNGIDSDEMWSVDAGSFYCNEIYFRTLSEIRSRN 251          
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Match: A0A7J6MCV1_PEROL (Uncharacterized protein n=6 Tax=Perkinsus olseni TaxID=32597 RepID=A0A7J6MCV1_PEROL)

HSP 1 Score: 88.6 bits (218), Expect = 1.100e-17
Identity = 59/164 (35.98%), Postives = 88/164 (53.66%), Query Frame = 1
Query:   91 RRAQVLLTGWGPFLNFSTNPSELVARRLARDGCSVEWDDALGSFELCFDVHVLPVNRSGVLRVQEELERRKKRKEEMPSAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAADILPSTADLGRMEVVGG-AVWSRNAGKYFCNELLFRSLHSIRSQ 579
            R+  V +TG+ PF +F+ NPS  VAR L    C+ ++         C     LPV  +G  +    L      K   P+A++HLGLE+ A GL++E    N  A  +   P+   +     ILPSTA+LG ++VV    +WS +AG Y+CNE+ FR+L  IR++
Sbjct:  112 RKRVVWVTGYQPFKDFTFNPSAAVARTL-NGSCTHDY---------CVQAFELPVTHAGASQPASWLREPAVPK---PAAIVHLGLEDRAKGLKLEVAAKNVLAESNSSLPI---LANGTSILPSTANLGLIDVVDADEMWSVDAGSYYCNEIYFRTLSEIRTR 259          
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Match: A0A812R9L8_9DINO (Pcp protein n=1 Tax=Symbiodinium natans TaxID=878477 RepID=A0A812R9L8_9DINO)

HSP 1 Score: 82.8 bits (203), Expect = 5.190e-16
Identity = 59/160 (36.88%), Postives = 90/160 (56.25%), Query Frame = 1
Query:  103 VLLTGWGPFLNFSTNPSELVARRLARDGCSVEWDDALGSFELCFDVHVLPVNRSGVLRVQEELERRKKRKEEMPSAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAADILPSTADLGRMEVVGG---AVWSRNAGKYFCNELLFRSLHSIR 573
            +LLTG+ P+ NF+ NPS  VA  L  +G  VE         L      + VN +GVL+ QE ++      +    A++HLG E+ A GL++ET+ AN  A   G   +  A P   ++LP+T DLG + +       +WSR+AG +FCNE+ +R+L+S+R
Sbjct:   24 ILLTGYNPWANFTQNPSGEVAAAL--NGSIVE--------RLMVHSIRVDVNEAGVLQAQEMVQ------QSGWDAIVHLGFEDEAKGLKLETMAANKRALHKG--KVASAGP---ELLPTTGDLGAVALNTNNPHELWSRDAGDFFCNEIYYRTLYSVR 162          
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Match: A0A813D6D7_POLGL (Hypothetical protein n=2 Tax=Polarella glacialis TaxID=89957 RepID=A0A813D6D7_POLGL)

HSP 1 Score: 82.4 bits (202), Expect = 1.140e-15
Identity = 61/165 (36.97%), Postives = 91/165 (55.15%), Query Frame = 1
Query:   97 AQVLLTGWGPFLNFSTNPSELVARRLARDGCSVEWDDALGSFELCFDVHVLPVNRSGVLRVQEELERRKKRKEEMPSAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAADILPSTADLGRMEVVGG---AVWSRNAGKYFCNELLFRSLHSIRSQN 582
            A VLLTG+ P+ NF+ NPS  VA  L  +G +V          L    + L V+ +GVL  +E +  R         A++HLG E+ A GL++ET+  N  A   G     P +P    +LP+TADLG + +       +WSR+AG +FCNE+ +RSL +IR+++
Sbjct:   24 ADVLLTGYNPWGNFTENPSGEVADVL--NGTTVSG--------LPIVAYRLDVSEAGVLAAEELVASRDW------DAIVHLGFEDSAKGLKLETMALNQRALHQG-----PVVPLGPLLLPTTADLGAVAMNTHNPHELWSRDAGTFFCNEIYYRSLSAIRTRS 167          
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Match: A0A1Q9EHE9_SYMMI (Pyrrolidone-carboxylate peptidase n=5 Tax=Symbiodinium TaxID=2949 RepID=A0A1Q9EHE9_SYMMI)

HSP 1 Score: 80.9 bits (198), Expect = 1.940e-15
Identity = 60/162 (37.04%), Postives = 86/162 (53.09%), Query Frame = 1
Query:   97 AQVLLTGWGPFLNFSTNPSELVARRLARDGCSVEWDDALGSFELCFDVHVLPVNRSGVLRVQEELERRKKRKEEMPSAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAADILPSTADLGRMEVVGG---AVWSRNAGKYFCNELLFRSLHSIR 573
            A +LLTG+ P+ N + NPS  VA  L  +G  VE        +L      + V   GVL  Q+ +ER          AV+HLG E+ A GL++ET+ AN  A   G        P    +LP+T+DLG + +       +WSR+AG +FCNE+ +R+L+SIR
Sbjct:   17 AAILLTGYNPWGNLTQNPSGEVAAAL--NGSIVE--------QLVVHSLRVDVTELGVLEAQDVVERGGW------DAVVHLGFEDEAKGLKLETMAANVRALHKG-----KVAPEGPQLLPTTSDLGAVALNTQNPHELWSRDAGNFFCNEIYYRTLYSIR 157          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig86168.20081.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 20
Match NameE-valueIdentityDescription
A0A813AE61_9DINO2.450e-2546.88Hypothetical protein n=1 Tax=Symbiodinium sp. KB8 ... [more]
A0A5A8CW37_CAFRO2.760e-2535.92Threonylcarbamoyl-AMP synthase n=2 Tax=Cafeteria r... [more]
A0A5A8C2M7_CAFRO2.870e-2535.92Threonylcarbamoyl-AMP synthase n=1 Tax=Cafeteria r... [more]
A0A5A8DUV4_CAFRO2.880e-2535.92Threonylcarbamoyl-AMP synthase n=1 Tax=Cafeteria r... [more]
A0A0D2UEL8_CAPO32.120e-2234.36Uncharacterized protein n=1 Tax=Capsaspora owczarz... [more]
C5KHG6_PERM56.780e-1935.98Pyrrolidone-carboxylate peptidase, putative n=1 Ta... [more]
A0A7J6MCV1_PEROL1.100e-1735.98Uncharacterized protein n=6 Tax=Perkinsus olseni T... [more]
A0A812R9L8_9DINO5.190e-1636.88Pcp protein n=1 Tax=Symbiodinium natans TaxID=8784... [more]
A0A813D6D7_POLGL1.140e-1536.97Hypothetical protein n=2 Tax=Polarella glacialis T... [more]
A0A1Q9EHE9_SYMMI1.940e-1537.04Pyrrolidone-carboxylate peptidase n=5 Tax=Symbiodi... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig86168contigM-pyrifera_M_contig86168:2..583 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score107.1
Seed ortholog evalue9.6e-21
Seed eggNOG ortholog192875.XP_004348153.1
Hectar predicted targeting categorychloroplast
EggNOG OGs2E8W4@1,2SFAX@2759
Ec32 ortholog descriptionPeptidase C15, pyroglutamyl peptidase I-like
Ec32 orthologEc-06_002440.1
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons1
Model size582
Cds size582
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816772.2431614-CDS-M-pyrifera_M_contig86168:1..5831622816772.2431614-CDS-M-pyrifera_M_contig86168:1..583Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig86168 2..583 -
1692278169.4686325-CDS-M-pyrifera_M_contig86168:1..5831692278169.4686325-CDS-M-pyrifera_M_contig86168:1..583Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig86168 2..583 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig86168.20081.1prot_M-pyrifera_M_contig86168.20081.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig86168 2..583 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig86168.20081.1

>prot_M-pyrifera_M_contig86168.20081.1 ID=prot_M-pyrifera_M_contig86168.20081.1|Name=mRNA_M-pyrifera_M_contig86168.20081.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=194bp
VVLCVSVAVVSVVGGGVLAFAPNLGGLGHHRRAQVLLTGWGPFLNFSTNP
SELVARRLARDGCSVEWDDALGSFELCFDVHVLPVNRSGVLRVQEELERR
KKRKEEMPSAVLHLGLENVALGLRMETVGANFEANPSGGEPLGPAIPGAA
DILPSTADLGRMEVVGGAVWSRNAGKYFCNELLFRSLHSIRSQN
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mRNA from alignment at M-pyrifera_M_contig86168:2..583-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig86168.20081.1 ID=mRNA_M-pyrifera_M_contig86168.20081.1|Name=mRNA_M-pyrifera_M_contig86168.20081.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=582bp|location=Sequence derived from alignment at M-pyrifera_M_contig86168:2..583- (Macrocystis pyrifera P11B4 male)
GTGGTGTTGTGCGTATCTGTGGCGGTGGTGAGCGTGGTTGGTGGGGGAGT ATTGGCGTTTGCGCCGAATTTAGGGGGGTTGGGGCATCATCGTCGGGCAC AGGTGCTACTGACAGGATGGGGTCCCTTTCTTAACTTTTCAACCAACCCG TCGGAGCTTGTGGCCCGAAGACTAGCTCGTGACGGTTGTAGTGTTGAGTG GGATGATGCATTGGGCAGCTTTGAGTTATGCTTTGATGTGCATGTGTTGC CTGTGAATCGCAGCGGAGTGCTGCGGGTGCAGGAGGAGCTTGAACGACGC AAGAAGCGAAAAGAAGAGATGCCAAGCGCCGTTTTACACCTTGGTCTGGA GAATGTGGCTCTTGGGCTGCGAATGGAAACAGTTGGGGCCAATTTTGAGG CGAATCCTAGTGGTGGAGAACCATTAGGGCCCGCTATTCCAGGCGCAGCC GACATTTTGCCTTCAACCGCGGATTTGGGCCGTATGGAGGTTGTTGGGGG GGCTGTATGGTCCCGTAATGCTGGAAAGTACTTTTGTAATGAGCTATTGT TCCGTTCATTGCATTCGATTCGCTCCCAGAAC
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig86168:2..583-

>mRNA_M-pyrifera_M_contig86168.20081.1 ID=mRNA_M-pyrifera_M_contig86168.20081.1|Name=mRNA_M-pyrifera_M_contig86168.20081.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1164bp|location=Sequence derived from alignment at M-pyrifera_M_contig86168:2..583- (Macrocystis pyrifera P11B4 male)
GTGGTGTTGTGCGTATCTGTGGCGGTGGTGAGCGTGGTTGGTGGGGGAGT
ATTGGCGTTTGCGCCGAATTTAGGGGGGTTGGGGCATCATCGTCGGGCAC
AGGTGCTACTGACAGGATGGGGTCCCTTTCTTAACTTTTCAACCAACCCG
TCGGAGCTTGTGGCCCGAAGACTAGCTCGTGACGGTTGTAGTGTTGAGTG
GGATGATGCATTGGGCAGCTTTGAGTTATGCTTTGATGTGCATGTGTTGC
CTGTGAATCGCAGCGGAGTGCTGCGGGTGCAGGAGGAGCTTGAACGACGC
AAGAAGCGAAAAGAAGAGATGCCAAGCGCCGTTTTACACCTTGGTCTGGA
GAATGTGGCTCTTGGGCTGCGAATGGAAACAGTTGGGGCCAATTTTGAGG
CGAATCCTAGTGGTGGAGAACCATTAGGGCCCGCTATTCCAGGCGCAGCC
GACATTTTGCCTTCAACCGCGGATTTGGGCCGTATGGAGGTTGTTGGGGG
GGCTGTATGGTCCCGTAATGCTGGAAAGTACTTTTGTAATGAGCTATTGT
TCCGTTCATTGCATTCGATTCGCTCCCAGAACGTGGTGTTGTGCGTATCT
GTGGCGGTGGTGAGCGTGGTTGGTGGGGGAGTATTGGCGTTTGCGCCGAA
TTTAGGGGGGTTGGGGCATCATCGTCGGGCACAGGTGCTACTGACAGGAT
GGGGTCCCTTTCTTAACTTTTCAACCAACCCGTCGGAGCTTGTGGCCCGA
AGACTAGCTCGTGACGGTTGTAGTGTTGAGTGGGATGATGCATTGGGCAG
CTTTGAGTTATGCTTTGATGTGCATGTGTTGCCTGTGAATCGCAGCGGAG
TGCTGCGGGTGCAGGAGGAGCTTGAACGACGCAAGAAGCGAAAAGAAGAG
ATGCCAAGCGCCGTTTTACACCTTGGTCTGGAGAATGTGGCTCTTGGGCT
GCGAATGGAAACAGTTGGGGCCAATTTTGAGGCGAATCCTAGTGGTGGAG
AACCATTAGGGCCCGCTATTCCAGGCGCAGCCGACATTTTGCCTTCAACC
GCGGATTTGGGCCGTATGGAGGTTGTTGGGGGGGCTGTATGGTCCCGTAA
TGCTGGAAAGTACTTTTGTAATGAGCTATTGTTCCGTTCATTGCATTCGA
TTCGCTCCCAGAAC
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