mRNA_M-pyrifera_M_contig85240.19869.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig85240.19869.1
Unique NamemRNA_M-pyrifera_M_contig85240.19869.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Match: D8S0W8_SELML (Epimerase domain-containing protein n=2 Tax=Selaginella moellendorffii TaxID=88036 RepID=D8S0W8_SELML)

HSP 1 Score: 95.5 bits (236), Expect = 7.980e-20
Identity = 60/127 (47.24%), Postives = 77/127 (60.63%), Query Frame = 1
Query:    1 PGATERLRLVEMDLLDAASVREAIKACHAVAHTASPFPPSNVTDPETTLYRPALLGTKNVLEACAATPSVTRVVVTSTAGTVYVRPDK--DAIVFDGTQWSSLSFARAKKMQYIESKLRADRAAHHF 375
            PGA ERL LVE DLL   +  + +K C  V HTASPF  + VTDPE  L +PA+ GT NVLEAC+ +PSV +VVVTS+   V   P +  D +V D + +S   + R  K  YI SK  A++ A  F
Sbjct:   52 PGAEERLELVEADLLKDGAFNDVVKDCQGVFHTASPFFLAGVTDPERQLIQPAVQGTLNVLEACSRSPSVAKVVVTSSTAAVAYNPKRTPDTVV-DESCFSDPDYCREMKAWYILSKTLAEQEAWKF 177          
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Match: A0A6I9QA19_ELAGV (cinnamoyl-CoA reductase 2 n=1 Tax=Elaeis guineensis var. tenera TaxID=51953 RepID=A0A6I9QA19_ELAGV)

HSP 1 Score: 94.0 bits (232), Expect = 5.770e-19
Identity = 58/126 (46.03%), Postives = 76/126 (60.32%), Query Frame = 1
Query:    4 GATERLRLVEMDLLDAASVREAIKACHAVAHTASPFPPSNVTDPETTLYRPALLGTKNVLEACAATPSVTRVVVTSTAGTVYV--RPDKDAIVFDGTQWSSLSFARAKKMQYIESKLRADRAAHHF 375
            GA ERL L + +LL+  S    I+ C  V HTASPF   +  DP+T L  PA+ GT NVL +C  TPSV RVVVTS+  TVY   RP    ++ D T +SS+ F + +K+ Y  SK+ A+ AA  F
Sbjct:  118 GANERLHLFKANLLEEGSFASVIEGCEGVFHTASPFY-MHAADPQTELIDPAVKGTLNVLSSCVKTPSVRRVVVTSSMATVYYNGRPRTPNVIVDETWFSSVEFCKQEKLWYQLSKILAEEAAWKF 242          
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Match: UPI000901F38A (cinnamoyl-CoA reductase 1-like n=3 Tax=Ipomoea TaxID=4119 RepID=UPI000901F38A)

HSP 1 Score: 92.0 bits (227), Expect = 2.110e-18
Identity = 58/127 (45.67%), Postives = 73/127 (57.48%), Query Frame = 1
Query:    4 GATERLRLVEMDLLDAASVREAIKACHAVAHTASPFPPSNVTDPETTLYRPALLGTKNVLEACAATPSVTRVVVTSTAGTVYV--RPDKDAIVFDGTQWSSLSFARAKKMQYIESKLRADRAAHHFL 378
            GA ERL L + DLL+  S    +  C  V HTASPF   NVTDP+  L  PA+ GT NVL +CA TPSV +VVVTS+   V    +P    +V D T WS+  F +  K  Y+ SK  A+ AA  F+
Sbjct:   81 GAKERLHLFKADLLEEGSFDSVVAGCEGVFHTASPFY-HNVTDPQAELIDPAVKGTLNVLGSCAKTPSVQKVVVTSSIAAVAYNGKPRTPEVVVDETWWSAADFCKEMKKWYVLSKTLAEDAAWKFV 206          
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Match: A0A7S4I653_9EUKA (Hypothetical protein (Fragment) n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4I653_9EUKA)

HSP 1 Score: 91.7 bits (226), Expect = 2.310e-18
Identity = 57/120 (47.50%), Postives = 70/120 (58.33%), Query Frame = 1
Query:    4 GATERLRLVEMDLLDAASVREAIKACHAVAHTASPFPPSNVTDPETTLYRPALLGTKNVLEACAATPSVTRVVVTSTAGTVYVRPDKDAIVFDGTQWSSLSFARAKKMQYIESKLRADRA 363
            GA ERL+L E DLL   S  +A + C  V HTASPF  S VTDPE+ + RPA+ GT +VL +   TPSV RVVVTS+   V V        F    WS+  F +  KM Y+ SK  A+RA
Sbjct:   49 GAKERLKLFEADLLQEGSFDDAFRHCSGVFHTASPFFFSGVTDPESQMLRPAVEGTISVLRSVCRTPSVKRVVVTSSLAAVIVGNYSPDHKFTEEDWSNAEFQKTAKMWYLLSKNLAERA 168          
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Match: A0A2Z7DB39_9LAMI (Cinnamoyl-CoA reductase 1-like n=1 Tax=Dorcoceras hygrometricum TaxID=472368 RepID=A0A2Z7DB39_9LAMI)

HSP 1 Score: 91.3 bits (225), Expect = 2.600e-18
Identity = 57/127 (44.88%), Postives = 74/127 (58.27%), Query Frame = 1
Query:    4 GATERLRLVEMDLLDAASVREAIKACHAVAHTASPFPPSNVTDPETTLYRPALLGTKNVLEACAATPSVTRVVVTSTAGTVYV--RPDKDAIVFDGTQWSSLSFARAKKMQYIESKLRADRAAHHFL 378
            GA ERL+L++ +LL+  S    +  C  V HTASPF    VTDP+  L  PAL GT NVL +CA TPSV RVV+TS+   V    +P    +V D T WS   F R  ++ Y+ SK  A+ AA  F+
Sbjct:   49 GAKERLKLIKANLLEEGSFDAVVDGCDGVFHTASPFY-HQVTDPQAELIDPALKGTLNVLGSCAKTPSVKRVVLTSSVAAVAFNGKPRSPEVVIDETWWSDPEFCRQTQLWYVLSKTLAEDAAWKFV 174          
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Match: A0A6I9T825_SESIN (cinnamoyl-CoA reductase 1 n=1 Tax=Sesamum indicum TaxID=4182 RepID=A0A6I9T825_SESIN)

HSP 1 Score: 91.3 bits (225), Expect = 2.770e-18
Identity = 60/128 (46.88%), Postives = 76/128 (59.38%), Query Frame = 1
Query:    4 GATERLRLVEMDLLDAASVREAIKACHAVAHTASPF--PPSNVTDPETTLYRPALLGTKNVLEACAATPSVTRVVVTSTAGTVYV--RPDKDAIVFDGTQWSSLSFARAKKMQYIESKLRADRAAHHF 375
            GA ERL+L++ +LLD  S   A+  C  V HTASPF  P  N   P+  L  PAL GT NVL +CA +PSV RVV+T++ G+VYV  R     +V D T WS L F R  +  Y  SK  A++AA  F
Sbjct:   49 GAKERLQLIKANLLDEGSFDAAVDGCDGVFHTASPFHDPGDN---PQAELIDPALKGTLNVLGSCAKSPSVKRVVLTASVGSVYVNERTLSPEVVIDETWWSDLDFCRRTQQWYSLSKTLAEQAAWKF 173          
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Match: UPI001CC61157 (phenylacetaldehyde reductase-like n=1 Tax=Telopea speciosissima TaxID=54955 RepID=UPI001CC61157)

HSP 1 Score: 90.9 bits (224), Expect = 3.790e-18
Identity = 62/126 (49.21%), Postives = 74/126 (58.73%), Query Frame = 1
Query:    4 GATERLRLVEMDLLDAASVREAIKACHAVAHTASPFPPSNVTDPETTLYRPALLGTKNVLEACAATPSVTRVVVTSTAGTV-YVR-PDKDAIVFDGTQWSSLSFARAKKMQYIESKLRADRAAHHF 375
            GA ERL L + +LL+  S   AI  C  V HTASPF  + VTDP+  L  PAL GT NVLE+CA TPSV R+VVTS+   V Y R P    +V D T +S   F R  K  Y+ SK  AD A+  F
Sbjct:   53 GAKERLHLFKANLLEEGSFDSAIDGCEGVFHTASPFYHA-VTDPQAELLDPALKGTLNVLESCAKTPSVKRMVVTSSVAAVAYNRTPRTPDVVVDETWYSDAEFCRENKSWYLLSKTLADEASWKF 177          
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Match: V7BZT2_PHAVU (Epimerase domain-containing protein n=7 Tax=Phaseoleae TaxID=163735 RepID=V7BZT2_PHAVU)

HSP 1 Score: 90.5 bits (223), Expect = 5.310e-18
Identity = 55/125 (44.00%), Postives = 76/125 (60.80%), Query Frame = 1
Query:    4 GATERLRLVEMDLLDAASVREAIKACHAVAHTASPFPPSNVTDPETTLYRPALLGTKNVLEACAATPSVTRVVVTSTAGTVYVRPDKDA-IVFDGTQWSSLSFARAKKMQYIESKLRADRAAHHF 375
            GATERL+LV+ DL++  S   AI  C  V H ASP   S ++DP+T +  PA+ GT NVL +C    ++ RVV+TS++ T+ +R D D  I  D + WSSL F    +  Y  SK+ A+RAA  F
Sbjct:   50 GATERLQLVQADLMEEGSFDNAIMGCKGVFHVASPVL-SIISDPKTQILEPAVKGTLNVLRSCRKNAALGRVVLTSSSSTLRIRDDFDPNIPLDESSWSSLEFCEKLQAWYAMSKIEAERAAWEF 173          
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Match: A0A8F5AR19_OLEEU (Phenylacetaldehyde reductase 1.1 n=1 Tax=Olea europaea TaxID=4146 RepID=A0A8F5AR19_OLEEU)

HSP 1 Score: 90.5 bits (223), Expect = 5.650e-18
Identity = 57/127 (44.88%), Postives = 75/127 (59.06%), Query Frame = 1
Query:    4 GATERLRLVEMDLLDAASVREAIKACHAVAHTASPFPPSNVTDPETTLYRPALLGTKNVLEACAATPSVTRVVVTSTAGTVYV--RPDKDAIVFDGTQWSSLSFARAKKMQYIESKLRADRAAHHFL 378
            GA ERL L++ +LL+  S    +  C  V HTASPF  + VTDP+  L  PAL GT NVLE+CA TPSV RVV+TS+   V    +P    ++ D T WS+  F +  K  Y+ SK  A+ AA  F+
Sbjct:   53 GAKERLHLLKANLLEQGSFDAVVDGCEGVFHTASPFFHA-VTDPQAELIDPALKGTLNVLESCAKTPSVKRVVLTSSIAAVAYNGKPRTPEVIVDETWWSNPEFCKEMKQWYVLSKTLAEDAAWKFV 178          
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Match: UPI001C4E3065 (phenylacetaldehyde reductase-like n=1 Tax=Macadamia integrifolia TaxID=60698 RepID=UPI001C4E3065)

HSP 1 Score: 89.7 bits (221), Expect = 1.010e-17
Identity = 60/127 (47.24%), Postives = 75/127 (59.06%), Query Frame = 1
Query:    4 GATERLRLVEMDLLDAASVREAIKACHAVAHTASPFPPSNVTDPETTLYRPALLGTKNVLEACAATPSVTRVVVTST-AGTVYVR-PDKDAIVFDGTQWSSLSFARAKKMQYIESKLRADRAAHHFL 378
            GA ERL L + +LL+  S    +  C  V HTASPF  + VTDP+  L  PAL GT NVLE+CA TPS+ RVVVTS+ A   Y R P    +V D T +S   F R  K+ Y+ SK  AD A+  F+
Sbjct:   53 GAKERLHLFKANLLEEGSFDSLVDGCTGVFHTASPFYHA-VTDPQAELLDPALKGTLNVLESCAKTPSIKRVVVTSSLAAVAYNRTPRTPDVVVDETWYSDAEFCRENKVWYLLSKTLADEASWKFV 178          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig85240.19869.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D8S0W8_SELML7.980e-2047.24Epimerase domain-containing protein n=2 Tax=Selagi... [more]
A0A6I9QA19_ELAGV5.770e-1946.03cinnamoyl-CoA reductase 2 n=1 Tax=Elaeis guineensi... [more]
UPI000901F38A2.110e-1845.67cinnamoyl-CoA reductase 1-like n=3 Tax=Ipomoea Tax... [more]
A0A7S4I653_9EUKA2.310e-1847.50Hypothetical protein (Fragment) n=1 Tax=Vannella s... [more]
A0A2Z7DB39_9LAMI2.600e-1844.88Cinnamoyl-CoA reductase 1-like n=1 Tax=Dorcoceras ... [more]
A0A6I9T825_SESIN2.770e-1846.88cinnamoyl-CoA reductase 1 n=1 Tax=Sesamum indicum ... [more]
UPI001CC611573.790e-1849.21phenylacetaldehyde reductase-like n=1 Tax=Telopea ... [more]
V7BZT2_PHAVU5.310e-1844.00Epimerase domain-containing protein n=7 Tax=Phaseo... [more]
A0A8F5AR19_OLEEU5.650e-1844.88Phenylacetaldehyde reductase 1.1 n=1 Tax=Olea euro... [more]
UPI001C4E30651.010e-1747.24phenylacetaldehyde reductase-like n=1 Tax=Macadami... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig85240contigM-pyrifera_M_contig85240:3..800 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score79.7
Seed ortholog evalue1.3e-12
Seed eggNOG ortholog102107.XP_008235846.1
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0003006,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005783,GO:0006725,GO:0007275,GO:0008150,GO:0008152,GO:0009058,GO:0009555,GO:0009653,GO:0009791,GO:0009987,GO:0010154,GO:0010208,GO:0010584,GO:0010927,GO:0012505,GO:0016043,GO:0019438,GO:0022414,GO:0022607,GO:0030198,GO:0032501,GO:0032502,GO:0032989,GO:0043062,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044085,GO:0044237,GO:0044249,GO:0044424,GO:0044444,GO:0044464,GO:0045229,GO:0048229,GO:0048316,GO:0048608,GO:0048646,GO:0048731,GO:0048856,GO:0048869,GO:0061458,GO:0071840,GO:0080110,GO:0085029
EggNOG free text desc.Tetraketide alpha-pyrone reductase
EggNOG OGs37J0J@33090,3G9PM@35493,4JF4V@91835,COG0451@1,KOG1502@2759
COG Functional cat.V
Best tax levelfabids
Best eggNOG OGNA|NA|NA
Exons2
Model size652
Cds size459
Stop1
Start1
Relationships

The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig85240.19869.1prot_M-pyrifera_M_contig85240.19869.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig85240 36..640 +


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816760.994228-UTR-M-pyrifera_M_contig85240:2..351622816760.994228-UTR-M-pyrifera_M_contig85240:2..35Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig85240 3..35 +
1692278162.6507735-UTR-M-pyrifera_M_contig85240:2..351692278162.6507735-UTR-M-pyrifera_M_contig85240:2..35Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig85240 3..35 +
1622816761.0306478-UTR-M-pyrifera_M_contig85240:640..8001622816761.0306478-UTR-M-pyrifera_M_contig85240:640..800Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig85240 641..800 +
1692278162.6871703-UTR-M-pyrifera_M_contig85240:640..8001692278162.6871703-UTR-M-pyrifera_M_contig85240:640..800Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig85240 641..800 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816761.0053418-CDS-M-pyrifera_M_contig85240:35..3771622816761.0053418-CDS-M-pyrifera_M_contig85240:35..377Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig85240 36..377 +
1692278162.6629546-CDS-M-pyrifera_M_contig85240:35..3771692278162.6629546-CDS-M-pyrifera_M_contig85240:35..377Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig85240 36..377 +
1622816761.0174215-CDS-M-pyrifera_M_contig85240:523..6401622816761.0174215-CDS-M-pyrifera_M_contig85240:523..640Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig85240 524..640 +
1692278162.6748912-CDS-M-pyrifera_M_contig85240:523..6401692278162.6748912-CDS-M-pyrifera_M_contig85240:523..640Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig85240 524..640 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig85240.19869.1

>prot_M-pyrifera_M_contig85240.19869.1 ID=prot_M-pyrifera_M_contig85240.19869.1|Name=mRNA_M-pyrifera_M_contig85240.19869.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=153bp
MDLLDAASVREAIKACHAVAHTASPFPPSNVTDPETTLYRPALLGTKNVL
EACAATPSVTRVVVTSTAGTVYVRPDKDAIVFDGTQWSSLSFARAKKMQY
IESKLRADRAAHHFLSIALFVRHSASHAGAWSNVGTPPWSLAAQALVGAD
WW*
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mRNA from alignment at M-pyrifera_M_contig85240:3..800+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig85240.19869.1 ID=mRNA_M-pyrifera_M_contig85240.19869.1|Name=mRNA_M-pyrifera_M_contig85240.19869.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=798bp|location=Sequence derived from alignment at M-pyrifera_M_contig85240:3..800+ (Macrocystis pyrifera P11B4 male)
CCTGGTGCCACGGAGCGACTTCGATTGGTGGAGATGGACTTGCTCGATGC AGCCAGTGTTCGGGAGGCGATCAAGGCGTGTCACGCTGTGGCACACACAG CTTCTCCGTTTCCTCCTTCCAACGTGACCGATCCCGAAACCACACTCTAC CGCCCTGCTCTCCTTGGCACCAAGAACGTTCTCGAAGCATGTGCTGCCAC TCCATCCGTCACTCGAGTCGTGGTTACTTCCACCGCTGGAACCGTCTATG TGCGTCCTGACAAGGACGCGATTGTGTTCGACGGCACACAGTGGTCCTCG CTCTCCTTTGCCAGGGCCAAGAAGATGCAGTACATCGAATCCAAGCTCAG AGCTGACCGTGCTGCACACCACTTTGTTCAAACCGGATCCTGGCCAGAGG ATGAGGCGATGGAGGTTGGAGAAGGAGGAGACAAGCTGCCGGAAGAGAGT GGTGGAGGTGAGGAATCTGCTCTACCAACTCCATCCACCCCGGCATCGCC TTCTCTTGGCCCGATTGGCAGCTCTCCATTGCACTTTTCGTACGCCACTC TGCATCCCACGCTGGTGCTTGGTCCAATGTTGGGACCCCGCCTTGGAGCC TCGCAGCACAAGCTCTTGTCGGTGCTGACTGGTGGTGAAGAGGAAGGGCG AGTTCCCAACGTGTGCAAGTGTTTTGTAGACGTGCGCGATGTTGCAGAAG CGCACGCAGCTGCGATTGAGAAAGACTTTTTGACTGGTCGTTTCTTGCTC ATTGGAGACTGCATGCCTTGGAGGCGCGTTGTGGAAGTGGCCATGGAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig85240:3..800+

>mRNA_M-pyrifera_M_contig85240.19869.1 ID=mRNA_M-pyrifera_M_contig85240.19869.1|Name=mRNA_M-pyrifera_M_contig85240.19869.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=918bp|location=Sequence derived from alignment at M-pyrifera_M_contig85240:3..800+ (Macrocystis pyrifera P11B4 male)
ATGGACTTGCTCGATGCAGCCAGTGTTCGGGAGGCGATCAAGGCGTGTCA
CGCTGTGGCACACACAGCTTCTCCGTTTCCTCCTTCCAACGTGACCGATC
CCGAAACCACACTCTACCGCCCTGCTCTCCTTGGCACCAAGAACGTTCTC
GAAGCATGTGCTGCCACTCCATCCGTCACTCGAGTCGTGGTTACTTCCAC
CGCTGGAACCGTCTATGTGCGTCCTGACAAGGACGCGATTGTGTTCGACG
GCACACAGTGGTCCTCGCTCTCCTTTGCCAGGGCCAAGAAGATGCAGTAC
ATCGAATCCAAGCTCAGAGCTGACCGTGCTGCACACCACTTTATGGACTT
GCTCGATGCAGCCAGTGTTCGGGAGGCGATCAAGGCGTGTCACGCTGTGG
CACACACAGCTTCTCCGTTTCCTCCTTCCAACGTGACCGATCCCGAAACC
ACACTCTACCGCCCTGCTCTCCTTGGCACCAAGAACGTTCTCGAAGCATG
TGCTGCCACTCCATCCGTCACTCGAGTCGTGGTTACTTCCACCGCTGGAA
CCGTCTATGTGCGTCCTGACAAGGACGCGATTGTGTTCGACGGCACACAG
TGGTCCTCGCTCTCCTTTGCCAGGGCCAAGAAGATGCAGTACATCGAATC
CAAGCTCAGAGCTGACCGTGCTGCACACCACTTTCTCTCCATTGCACTTT
TCGTACGCCACTCTGCATCCCACGCTGGTGCTTGGTCCAATGTTGGGACC
CCGCCTTGGAGCCTCGCAGCACAAGCTCTTGTCGGTGCTGACTGGTGGTG
ACTCTCCATTGCACTTTTCGTACGCCACTCTGCATCCCACGCTGGTGCTT
GGTCCAATGTTGGGACCCCGCCTTGGAGCCTCGCAGCACAAGCTCTTGTC
GGTGCTGACTGGTGGTGA
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