mRNA_M-pyrifera_M_contig84946.19793.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig84946.19793.1
Unique NamemRNA_M-pyrifera_M_contig84946.19793.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Match: A0A3D1GBE7_9BACT (Esterase n=1 Tax=Balneolaceae bacterium TaxID=2053516 RepID=A0A3D1GBE7_9BACT)

HSP 1 Score: 191 bits (486), Expect = 7.490e-58
Identity = 96/165 (58.18%), Postives = 125/165 (75.76%), Query Frame = 1
Query:    1 EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKKEGAITVAVDLNAKASNKVMI-SKKRKRKTEVQKWFESLLPENILESQLKEGKKNDDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDFEKAKDIYKIGKEAY 492
            EVY+  G+LY ALRASV+IPG   PVV   KVLVDGGV NP+P+NLV+K+E AI VAVDLNA+A  K +  S     + + QKW+E LLP+ +LE   K+ K+ ++AFSL+GLMES+FS TQD+LTSLM ++Y+P+V I +PRNT NTFDF KAK++Y+ G  AY
Sbjct:  121 EVYFRDGNLYTALRASVSIPGLFTPVVLGNKVLVDGGVLNPVPVNLVNKQEDAIIVAVDLNARAEEKKLTESSLNSEEEKEQKWYERLLPDQVLEFAKKQDKQEEEAFSLLGLMESTFSFTQDRLTSLMFEMYKPEVRINIPRNTCNTFDFYKAKEVYEAGINAY 285          
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Match: UPI00036EC5A4 (patatin-like phospholipase family protein n=1 Tax=Gracilimonas tropica TaxID=454600 RepID=UPI00036EC5A4)

HSP 1 Score: 184 bits (468), Expect = 4.000e-55
Identity = 96/173 (55.49%), Postives = 127/173 (73.41%), Query Frame = 1
Query:    1 EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKKEGAITVAVDLNAKASNKVMISKKRKRKTEVQKWFESLLPENILESQLKEGKKN----DDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDFEKAKDIYKIGKEAYLDSVK 507
            EV++ SGDLYKALRASV+IPGF VPVV+DG+VLVDGGV NPLPINLV KKEGA  VAV+LN  A  K     +  R  E+QKWF+ +LPE I  ++  + K      +++FSLI LM+S+FS TQD+LT LM+++Y+PD LI +PRN    FDF++ + IYKIG++A+   +K
Sbjct:  121 EVHFKSGDLYKALRASVSIPGFFVPVVEDGQVLVDGGVLNPLPINLVDKKEGAEIVAVNLNGTADPKYDKPPEPDRLEEMQKWFDKMLPEAIKNNRKNKTKSELPAEENSFSLIELMDSTFSFTQDRLTELMIEMYKPDHLINIPRNACGVFDFDQGERIYKIGQDAFQKKMK 293          
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Match: UPI000363F890 (patatin-like phospholipase family protein n=1 Tax=Balneola vulgaris TaxID=287535 RepID=UPI000363F890)

HSP 1 Score: 184 bits (466), Expect = 9.730e-55
Identity = 88/172 (51.16%), Postives = 130/172 (75.58%), Query Frame = 1
Query:    1 EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKKEGAITVAVDLNAKASNKVMISKKRKRKTEVQKWFESLLPENILE---SQLKEGKKNDDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDFEKAKDIYKIGKEAYLDSVK 507
            EVY+  GDL+KALRASV+IPG   PVV DGKVLVDGGV NPLPINLV+K++ AI VAVD+N++   + ++ K+ +++     WFE+LLP +I E   S+ KE +   ++F+L  LMES+FS TQD+LT +M++ +QPD+L+++PRNT +TF+F KA+D+  +G+ AY  +++
Sbjct:  121 EVYFKEGDLFKALRASVSIPGIFTPVVYDGKVLVDGGVLNPLPINLVNKQDDAIVVAVDINSRTIIEELLLKRNEKEVHKPSWFENLLPNSIKELARSEPKEDEPEKESFNLFELMESTFSFTQDQLTKVMIEYHQPDILVRIPRNTCDTFEFHKARDLVNVGERAYKHAMQ 292          
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Match: A0A2D8CI20_9BACT (Esterase n=2 Tax=Balneolaceae TaxID=1813606 RepID=A0A2D8CI20_9BACT)

HSP 1 Score: 182 bits (462), Expect = 3.210e-54
Identity = 91/170 (53.53%), Postives = 128/170 (75.29%), Query Frame = 1
Query:    1 EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKKEGAITVAVDLNAKASNKVMISKKRKRKTEVQKWFESLLPENILESQLKEGKKNDDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDFEKAKDIYKIGKEAYLDSVKS 510
            EV++ SGDLYKALRASV+IPGF VPVV  G+VLVDGGV NPLPINLV K+E  I VAV+LN +   K     +  R  E++KWF  LLPE++ +++  E    +D+FSLI LM+S+FS TQD+LT LM+++Y+PD LI++PRN++  FDF++AK I ++G+++Y + + S
Sbjct:  121 EVHFKSGDLYKALRASVSIPGFFVPVVDHGRVLVDGGVLNPLPINLVDKEEDEIIVAVNLNGRYDPKYDKKPEPDRIDEMKKWFHDLLPESMKDNRKVEVDPEEDSFSLIELMDSTFSFTQDRLTRLMIEVYKPDYLIEIPRNSSGIFDFDQAKKILEVGRKSYHEVIDS 290          
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Match: UPI000CD91B44 (patatin-like phospholipase family protein n=1 Tax=Gracilimonas amylolytica TaxID=1749045 RepID=UPI000CD91B44)

HSP 1 Score: 181 bits (460), Expect = 8.010e-54
Identity = 96/172 (55.81%), Postives = 126/172 (73.26%), Query Frame = 1
Query:    1 EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKKEGAITVAVDLNAKASNKVMISKKRKRKT----EVQKWFESLLPENILESQLKEGKKN----DDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDFEKAKDIYKIGKEAY 492
            EV++T GDLYKALRASV+IPGF VPVV+DG VLVDGGV NPLP+NLV+K++ AI VAV+LN K   K    KK K +T    EVQKWF+ +LP++    +  + K      +++FSLI LM+S+FS TQD+LT LM+++YQPD LI +PRN    FDF++ K IY+IGK +Y
Sbjct:  120 EVHFTKGDLYKALRASVSIPGFFVPVVEDGMVLVDGGVLNPLPVNLVNKQDDAIIVAVNLNGKPDPK--FDKKPKEETDTFDEVQKWFDKMLPDSFKTKRKTKPKAELPAKEESFSLIELMDSTFSFTQDRLTELMIEMYQPDHLIDIPRNICGVFDFDQGKRIYEIGKRSY 289          
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Match: A0A2N0VGQ6_9BACT (Esterase n=2 Tax=Rhodohalobacter TaxID=1974209 RepID=A0A2N0VGQ6_9BACT)

HSP 1 Score: 176 bits (447), Expect = 6.320e-52
Identity = 89/173 (51.45%), Postives = 125/173 (72.25%), Query Frame = 1
Query:    1 EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKKEGAITVAVDLNAKASNKVMISKKRKRKTEVQKWFESLLPENILESQLKEGKK----NDDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDFEKAKDIYKIGKEAYLDSVK 507
            EV++  GDLYKALRASV++PGF VPVV++G+VLVDGGV NPLPINLV KKEGA  +AV+LN K   K     +  R  E+Q WF+ +LP+++  +   + K     ++D+FSLI LM+SSFS TQD+LT L++++Y+P+ LI++PRN    FDF++ K +Y+IG EA    +K
Sbjct:  121 EVHFKKGDLYKALRASVSMPGFFVPVVENGQVLVDGGVLNPLPINLVEKKEGAEIIAVNLNGKMDPKYDKPSEPDRLEEMQNWFDKILPDSMKTNHKTKAKSELPADEDSFSLIELMDSSFSFTQDRLTELIIELYKPNHLIEIPRNVCGVFDFDQGKRVYEIGMEACKSVIK 293          
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Match: A0A2D9FYK3_9BACT (Esterase n=2 Tax=Balneolaceae TaxID=1813606 RepID=A0A2D9FYK3_9BACT)

HSP 1 Score: 173 bits (439), Expect = 1.010e-50
Identity = 88/168 (52.38%), Postives = 123/168 (73.21%), Query Frame = 1
Query:    1 EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKKEGAITVAVDLNAKASNKVMISKKRKRKTEVQKWFESLLPENILESQLKEGK----KNDDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDFEKAKDIYKIGKEAY 492
            EV++  GDLYKALRASV+IPGF VPVV+DGKVLVDGGV NPLPINLV K++GA  VAV+LN + ++K     +  +  E+QKWF+ LLP+++   +  + K      +++FSLI LM+S+FS TQD+LT L +++Y PD LI++PRN    FDF++ + +  IGKEA+
Sbjct:  121 EVHFKKGDLYKALRASVSIPGFFVPVVEDGKVLVDGGVLNPLPINLVKKEKGAEIVAVNLNGRPNSKYDKPPEPDKLEEMQKWFDKLLPDSMKSKRKTKAKVELPAEEESFSLIELMDSTFSFTQDRLTELTIEMYNPDHLIEIPRNACGVFDFDQGERVCDIGKEAF 288          
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Match: A0A1B6Y7T6_9BACT (Esterase n=4 Tax=Bacteroidetes/Chlorobi group TaxID=68336 RepID=A0A1B6Y7T6_9BACT)

HSP 1 Score: 168 bits (425), Expect = 1.160e-48
Identity = 81/168 (48.21%), Postives = 126/168 (75.00%), Query Frame = 1
Query:    1 EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKKEGAITVAVDLNAKASNKVMISKKRKRKTEVQKWFESLLPENILESQLKEGKKNDDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDFEKAKDIYKIGKEAYLDSV 504
            EV++  GDL+KALRASV+IPG   P+V +  VLVDGGV NPLPINLV K++ AI VAVD+N++   + ++ K+ K++ + + WFE++LP++I E      +K  ++FSL  LMES+FS TQD+LT LM++ Y+PD+L++VPR+T +TF+F K+K + ++G  A+ +++
Sbjct:  122 EVHFKEGDLFKALRASVSIPGIFTPIVDEDLVLVDGGVLNPLPINLVQKRDDAIIVAVDINSRTIVEDLLIKEEKKQRK-KYWFENILPDSIKEFAQNHEEKKKESFSLFELMESTFSFTQDRLTELMIEHYKPDILVEVPRDTCDTFEFYKSKQLVEVGSRAFRNAL 288          
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Match: A0A846FAA4_9CYAN (Esterase n=1 Tax=Cyanothece sp. SIO1E1 TaxID=2607788 RepID=A0A846FAA4_9CYAN)

HSP 1 Score: 166 bits (421), Expect = 4.040e-48
Identity = 84/167 (50.30%), Postives = 121/167 (72.46%), Query Frame = 1
Query:    1 EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKKEGAITVAVDLNAKASNKVMISKKRKRKTEVQKWFESLLPENILESQLKEGKKNDDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDFEKAKDIYKIGKEAYLDS 501
            EV++ +GDLYKALR+SV+IPG  VPV +  K+LVDGGV NPLP+NLV +KEG   VAVDLNA+    +   K+++++    KW+E ++P+NILE+   E KK +D+ S+  LME +F  TQD+L  LM++ Y PD+L+ +PR T +TF+F KA+ IYK G +A+ +S
Sbjct:  121 EVHFKNGDLYKALRSSVSIPGVFVPVDESNKLLVDGGVLNPLPLNLVDRKEGVSVVAVDLNAEGKPLI---KQKEKEISPPKWYEGVVPKNILENSRFETKKAEDSLSVFELMEKTFVLTQDRLKDLMIKSYPPDLLVSIPRKTCSTFEFWKAEYIYKTGIKAFKNS 284          
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Match: A0A6A8Q5H7_9BACT (Esterase n=1 Tax=Balneolaceae bacterium TaxID=2053516 RepID=A0A6A8Q5H7_9BACT)

HSP 1 Score: 165 bits (417), Expect = 2.880e-47
Identity = 89/180 (49.44%), Postives = 127/180 (70.56%), Query Frame = 1
Query:    1 EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKKEGAITVAVDLNAKAS---NKVMISK-----KRKRKTEVQKWFESLLPENILE--SQLKEGKKNDDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDFEKAKDIYKIGKEAYLDSVKS 510
            EV++TSG+LYKALRASV+IPG  VPVV   ++LVDGGV NPLP+NLV K+E A+ VAVD+N K     ++V  S      K++ +  ++KWF+ L+P+ + E   +L+  K N  +FSL  LM+S+FS TQD+LT LM+Q+Y+PD LI +PRNT +TFDF +A+ +   G++ Y  S+ S
Sbjct:  126 EVHFTSGELYKALRASVSIPGVFVPVVDGNRILVDGGVLNPLPVNLVKKQEDAMVVAVDVNGKIIPDVDEVSASTSDPEDKQEEEDRLKKWFDDLIPDALHEYREKLENEKNNRPSFSLFELMDSTFSFTQDRLTDLMIQMYKPDHLITIPRNTCDTFDFHEAEMVIDQGRKFYHSSMDS 305          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig84946.19793.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3D1GBE7_9BACT7.490e-5858.18Esterase n=1 Tax=Balneolaceae bacterium TaxID=2053... [more]
UPI00036EC5A44.000e-5555.49patatin-like phospholipase family protein n=1 Tax=... [more]
UPI000363F8909.730e-5551.16patatin-like phospholipase family protein n=1 Tax=... [more]
A0A2D8CI20_9BACT3.210e-5453.53Esterase n=2 Tax=Balneolaceae TaxID=1813606 RepID=... [more]
UPI000CD91B448.010e-5455.81patatin-like phospholipase family protein n=1 Tax=... [more]
A0A2N0VGQ6_9BACT6.320e-5251.45Esterase n=2 Tax=Rhodohalobacter TaxID=1974209 Rep... [more]
A0A2D9FYK3_9BACT1.010e-5052.38Esterase n=2 Tax=Balneolaceae TaxID=1813606 RepID=... [more]
A0A1B6Y7T6_9BACT1.160e-4848.21Esterase n=4 Tax=Bacteroidetes/Chlorobi group TaxI... [more]
A0A846FAA4_9CYAN4.040e-4850.30Esterase n=1 Tax=Cyanothece sp. SIO1E1 TaxID=26077... [more]
A0A6A8Q5H7_9BACT2.880e-4749.44Esterase n=1 Tax=Balneolaceae bacterium TaxID=2053... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig84946contigM-pyrifera_M_contig84946:355..870 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score184.5
Seed ortholog evalue4.2e-44
Seed eggNOG ortholog1121930.AQXG01000005_gene655
Preferred namerssA
KEGG koko:K07001
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0016787
EggNOG free text desc.Patatin-like phospholipase
EggNOG OGs1ISPQ@117747,4NDXY@976,COG1752@1,COG1752@2
COG Functional cat.M
Best tax levelSphingobacteriia
Best eggNOG OGNA|NA|NA
BRITEko00000
Exons1
Model size516
Cds size516
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816756.959887-CDS-M-pyrifera_M_contig84946:354..8701622816756.959887-CDS-M-pyrifera_M_contig84946:354..870Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84946 355..870 -
1692278160.1858382-CDS-M-pyrifera_M_contig84946:354..8701692278160.1858382-CDS-M-pyrifera_M_contig84946:354..870Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84946 355..870 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig84946.19793.1prot_M-pyrifera_M_contig84946.19793.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig84946 355..870 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig84946.19793.1

>prot_M-pyrifera_M_contig84946.19793.1 ID=prot_M-pyrifera_M_contig84946.19793.1|Name=mRNA_M-pyrifera_M_contig84946.19793.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=172bp
EVYYTSGDLYKALRASVAIPGFIVPVVQDGKVLVDGGVSNPLPINLVHKK
EGAITVAVDLNAKASNKVMISKKRKRKTEVQKWFESLLPENILESQLKEG
KKNDDAFSLIGLMESSFSHTQDKLTSLMLQIYQPDVLIKVPRNTANTFDF
EKAKDIYKIGKEAYLDSVKSI*
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mRNA from alignment at M-pyrifera_M_contig84946:355..870-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig84946.19793.1 ID=mRNA_M-pyrifera_M_contig84946.19793.1|Name=mRNA_M-pyrifera_M_contig84946.19793.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=516bp|location=Sequence derived from alignment at M-pyrifera_M_contig84946:355..870- (Macrocystis pyrifera P11B4 male)
GAAGTATATTATACCTCTGGAGATTTGTACAAAGCTTTGCGGGCTTCGGT TGCAATTCCAGGTTTCATAGTTCCTGTTGTACAAGATGGAAAGGTGTTAG TTGATGGTGGAGTTTCTAACCCGTTACCAATAAATCTGGTACATAAAAAA GAAGGAGCAATTACAGTTGCGGTAGATTTAAATGCCAAAGCCAGTAACAA AGTGATGATTTCTAAAAAGAGAAAAAGGAAAACGGAAGTTCAGAAATGGT TTGAAAGTTTACTTCCTGAAAATATTCTAGAGAGTCAATTAAAAGAGGGA AAAAAGAACGATGATGCATTCTCACTTATAGGATTAATGGAAAGCTCTTT CAGTCATACTCAAGACAAGCTAACCTCCCTTATGCTTCAAATCTATCAGC CAGATGTATTGATTAAGGTTCCAAGAAATACTGCAAATACCTTCGACTTC GAAAAAGCAAAGGATATTTATAAAATTGGAAAAGAAGCTTATTTAGATTC AGTAAAATCCATCTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig84946:355..870-

>mRNA_M-pyrifera_M_contig84946.19793.1 ID=mRNA_M-pyrifera_M_contig84946.19793.1|Name=mRNA_M-pyrifera_M_contig84946.19793.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1032bp|location=Sequence derived from alignment at M-pyrifera_M_contig84946:355..870- (Macrocystis pyrifera P11B4 male)
GAAGTATATTATACCTCTGGAGATTTGTACAAAGCTTTGCGGGCTTCGGT
TGCAATTCCAGGTTTCATAGTTCCTGTTGTACAAGATGGAAAGGTGTTAG
TTGATGGTGGAGTTTCTAACCCGTTACCAATAAATCTGGTACATAAAAAA
GAAGGAGCAATTACAGTTGCGGTAGATTTAAATGCCAAAGCCAGTAACAA
AGTGATGATTTCTAAAAAGAGAAAAAGGAAAACGGAAGTTCAGAAATGGT
TTGAAAGTTTACTTCCTGAAAATATTCTAGAGAGTCAATTAAAAGAGGGA
AAAAAGAACGATGATGCATTCTCACTTATAGGATTAATGGAAAGCTCTTT
CAGTCATACTCAAGACAAGCTAACCTCCCTTATGCTTCAAATCTATCAGC
CAGATGTATTGATTAAGGTTCCAAGAAATACTGCAAATACCTTCGACTTC
GAAAAAGCAAAGGATATTTATAAAATTGGAAAAGAAGCTTATTTAGATTC
AGTAAAATCCATCTAAGAAGTATATTATACCTCTGGAGATTTGTACAAAG
CTTTGCGGGCTTCGGTTGCAATTCCAGGTTTCATAGTTCCTGTTGTACAA
GATGGAAAGGTGTTAGTTGATGGTGGAGTTTCTAACCCGTTACCAATAAA
TCTGGTACATAAAAAAGAAGGAGCAATTACAGTTGCGGTAGATTTAAATG
CCAAAGCCAGTAACAAAGTGATGATTTCTAAAAAGAGAAAAAGGAAAACG
GAAGTTCAGAAATGGTTTGAAAGTTTACTTCCTGAAAATATTCTAGAGAG
TCAATTAAAAGAGGGAAAAAAGAACGATGATGCATTCTCACTTATAGGAT
TAATGGAAAGCTCTTTCAGTCATACTCAAGACAAGCTAACCTCCCTTATG
CTTCAAATCTATCAGCCAGATGTATTGATTAAGGTTCCAAGAAATACTGC
AAATACCTTCGACTTCGAAAAAGCAAAGGATATTTATAAAATTGGAAAAG
AAGCTTATTTAGATTCAGTAAAATCCATCTAA
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