mRNA_M-pyrifera_M_contig84909.19790.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig84909.19790.1
Unique NamemRNA_M-pyrifera_M_contig84909.19790.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Match: A0A6J1HLJ3_CUCMA (protein MON2 homolog isoform X1 n=16 Tax=Cucurbitaceae TaxID=3650 RepID=A0A6J1HLJ3_CUCMA)

HSP 1 Score: 74.7 bits (182), Expect = 1.930e-12
Identity = 64/168 (38.10%), Postives = 93/168 (55.36%), Query Frame = 1
Query:   22 LESDLRALAQTARAGHPAVREVAERAIVKLRSIPPDAPFPPAETI-RPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVLSRQSEHPSETIRVRALQTLPLVL-SRSPPGSVGVSEIAAAMSLGLSMMRGD-SSGLLRGMAAATVRQAAGIAFD 516
            LESDLRAL+  AR  HPAV++ AE AI+KLRS+   +     E I R  LLAC      +    L    L SLQ+L + +++ PS    IL  L   +E   ET++++ LQT+ ++  SR  P S     +A A+ + + ++  + SS  +R  AAAT RQA  + FD
Sbjct:    7 LESDLRALSVEARRRHPAVKDGAEHAILKLRSMMSPSDIAENEDILRIFLLACE-----AKTIKLSVIGLSSLQKLISHDAVSPSALKEILSTLKDHAEISDETVQLKTLQTILIIFQSRLHPESE--ENMAQALGICIRLLENNRSSDSVRNTAAATFRQAVALIFD 167          
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Match: UPI000C1D6B28 (protein MON2 homolog isoform X1 n=2 Tax=Olea europaea var. sylvestris TaxID=158386 RepID=UPI000C1D6B28)

HSP 1 Score: 73.6 bits (179), Expect = 4.850e-12
Identity = 61/174 (35.06%), Postives = 92/174 (52.87%), Query Frame = 1
Query:    1 MTTPLRALESDLRALAQTARAGHPAVREVAERAIVKLRSIP-PDAPFPPAETIRPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVLSRQSEHPSETIRVRALQTLPLVLSRSPPGSVGVSEIAAAMSLGLSMMRGD-SSGLLRGMAAATVRQAAGIAFD 516
            M T +  LESDLRAL+  AR  +PAV++ AE AI+KLRS+  P+      + +R  L+AC           L    L  LQ+L + +++ PS    IL  L    E   E++++++LQT+ LV+ +S         +A A  + L ++    SS  +R  AAAT RQA  + FD
Sbjct:    1 MATFMAVLESDLRALSAEARRRYPAVKDAAEHAILKLRSLASPNEIAQDEDIVRIFLMACE-----VKNVKLSVIGLSCLQKLISHDAVAPSALNEILSTLKEHGEMADESVQLKSLQTI-LVIFQSHLRPDDEENMAHAFGICLRLLENSRSSDSVRNTAAATFRQAVALIFD 168          
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Match: A0A1U8N3B0_GOSHI (protein MON2 homolog n=21 Tax=Gossypium TaxID=3633 RepID=A0A1U8N3B0_GOSHI)

HSP 1 Score: 72.4 bits (176), Expect = 1.220e-11
Identity = 61/168 (36.31%), Postives = 92/168 (54.76%), Query Frame = 1
Query:   22 LESDLRALAQTARAGHPAVREVAERAIVKLRSIPPDAPFPPAETI-RPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVLSRQSEHPSETIRVRALQTLPLVL-SRSPPGSVGVSEIAAAMSLGLSMMRGD-SSGLLRGMAAATVRQAAGIAFD 516
            LESDLRAL+  AR  HPAV++ AE AI+KLR++   +     E I R  L+AC           L    L  LQ+L + +++ PS    IL  L   +E P E+++++ LQT+ ++  SR  P S     +A A+ + L ++  + SS  +R  AAAT RQA  + F+
Sbjct:    7 LESDLRALSAEARRRHPAVKDGAEHAILKLRTLSSPSEISLNEDIVRIFLMACE-----VKTVKLSVIGLSCLQKLISHDAVAPSVLNEILPTLKDHAEMPDESVQLKTLQTILIIFQSRLHPESE--ENMAKALGICLQLLENNRSSDSVRNTAAATFRQAVALVFE 167          
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Match: A0A0A9LWA6_ARUDO (Protein MON2 homolog n=1 Tax=Arundo donax TaxID=35708 RepID=A0A0A9LWA6_ARUDO)

HSP 1 Score: 72.0 bits (175), Expect = 1.580e-11
Identity = 60/170 (35.29%), Postives = 90/170 (52.94%), Query Frame = 1
Query:   13 LRALESDLRALAQTARAGHPAVREVAERAIVKLRSIPPDAPFPPAETI-RPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVLSRQSEHPSETIRVRALQTLPLVLSRSPPGSVGVSEIAAAMSLGLSMMRGD-SSGLLRGMAAATVRQAAGIAFD 516
            + ALE+DLRAL+  AR  HPAV++ AE AI+KLRS+   +     E I R  L+AC     G     L    L  LQ+L + +++  S    IL  L   +E   E ++++ LQT+ L+L +S         ++ A+ + L ++    SS  +R  AAAT RQA  + FD
Sbjct:    4 MAALEADLRALSAEARRRHPAVKDAAEHAILKLRSLSSPSEIAQNEDILRMFLMAC-----GVKSVKLSVIGLSCLQKLISHDAVASSALKEILATLKDHAEMTDEIVQLKTLQTM-LILFQSHLHPESEESMSQALGICLHLLESSRSSDSVRNTAAATFRQAVALVFD 167          
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Match: S8CYZ5_9LAMI (Uncharacterized protein (Fragment) n=1 Tax=Genlisea aurea TaxID=192259 RepID=S8CYZ5_9LAMI)

HSP 1 Score: 72.0 bits (175), Expect = 1.640e-11
Identity = 60/167 (35.93%), Postives = 89/167 (53.29%), Query Frame = 1
Query:   22 LESDLRALAQTARAGHPAVREVAERAIVKLRSIP-PDAPFPPAETIRPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVLSRQSEHPSETIRVRALQTLPLVLSRSPPGSVGVSEIAAAMSLGLSMMRGDSS-GLLRGMAAATVRQAAGIAFD 516
            LESDLRAL+  AR  +P+V++ AE AI+KLRS+  PD      + +R  ++AC           L    L  LQ+L + +++PPS    IL  L   SE   E+I+++ LQT+ LVL +S         +A A+ + L ++    S   +R  AAAT RQ   + FD
Sbjct:    6 LESDLRALSAEARRRYPSVKDAAEHAILKLRSLSSPDEIAHNEDILRIFVMACQ-----VRNAKLSVIGLSCLQKLISHDAVPPSAVKEILSTLKEHSEMADESIQLKTLQTV-LVLFQSRLQPDDEENVAYALGICLRLLENTRSLDSVRNTAAATFRQLVALIFD 166          
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Match: A0A5N6QSQ2_9ROSI (Uncharacterized protein n=2 Tax=Carpinus fangiana TaxID=176857 RepID=A0A5N6QSQ2_9ROSI)

HSP 1 Score: 72.0 bits (175), Expect = 1.660e-11
Identity = 61/167 (36.53%), Postives = 88/167 (52.69%), Query Frame = 1
Query:   22 LESDLRALAQTARAGHPAVREVAERAIVKLRSIPPDAPFPPAETI-RPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVLSRQSEHPSETIRVRALQTLPLVLSRSPPGSVGVSEIAAAMSLGLSMMRGDSS-GLLRGMAAATVRQAAGIAFD 516
            LESDLRAL+  AR  HPAV+E AE AI+KLRS+   +     E I R  L+AC     G     L    L  LQ+L + + + PS    IL  L   ++   ET++++ LQT+ L++ +S          A A+ + L ++  + S   +R  AAAT RQA  + FD
Sbjct:   15 LESDLRALSAEARRRHPAVKEGAEHAILKLRSLSSSSEIVHNEDILRIFLMAC-----GVRTVKLSVIGLSCLQKLISHDVVAPSALKEILSTLKDHADMADETVQLKTLQTI-LIIFQSRLHPENEENTAQALGICLQLLESNRSPDSVRNTAAATFRQAVALIFD 175          
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Match: A0A446Q1V0_TRITD (DCB domain-containing protein n=2 Tax=Triticum turgidum subsp. durum TaxID=4567 RepID=A0A446Q1V0_TRITD)

HSP 1 Score: 70.5 bits (171), Expect = 1.670e-11
Identity = 59/170 (34.71%), Postives = 92/170 (54.12%), Query Frame = 1
Query:   13 LRALESDLRALAQTARAGHPAVREVAERAIVKLRSIP-PDAPFPPAETIRPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVLSRQSEHPSETIRVRALQTLPLVLSRSPPGSVGVSEIAAAMSLGLSMMRGD-SSGLLRGMAAATVRQAAGIAFD 516
            + ALE+DLRAL+  AR  HPAV++ AE A +KLRS+  P       + +R  L+AC   S       L    L  LQ+L + +++  S    IL  L   SE P E+++++ LQT+ L++ +S        +++ A+ + L ++    SS  +R  AAAT RQA  + FD
Sbjct:    4 MAALEADLRALSAEARRRHPAVKDAAEHATLKLRSLSNPREIAQKGDILRIFLMACSVKS-----VKLSVIGLSCLQKLISHDAVASSALKEILATLRDHSEMPDESLQLKTLQTM-LIVFQSHLRPESEEDMSQALGICLHLLESSRSSDSVRNTAAATFRQAVALVFD 167          
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Match: A0A388KWM5_CHABU (Uncharacterized protein n=1 Tax=Chara braunii TaxID=69332 RepID=A0A388KWM5_CHABU)

HSP 1 Score: 72.0 bits (175), Expect = 1.670e-11
Identity = 56/167 (33.53%), Postives = 91/167 (54.49%), Query Frame = 1
Query:   22 LESDLRALAQTARAGHPAVREVAERAIVKLRSIP-PDAPFPPAETIRPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVLSRQSEHPSETIRVRALQTLPLVLSRSPPGSVGVSEIAAAMSLGLSMMRGD-SSGLLRGMAAATVRQAAGIAFD 516
            LE+DLR+L Q AR  +P V+E AERA++KLRS+  P++     + +R  ++AC      +    L    L  LQ+L   +++ P+  G IL VL    E   ET++++ LQT+ L + +S    V    +A  + + L ++    +S  +   AAAT+RQA  + FD
Sbjct:    9 LENDLRSLCQEARRKYPPVKEAAERALLKLRSVSDPESLAKSDDVMRTFVIACE-----TKNLKLTVIGLACLQKLIAHDAVAPAALGQILSVLKDSPESFDETVQLKTLQTV-LTILQSQLQPVDEDSMATLLGVCLRLLGSSRNSDSVHSTAAATLRQAVALIFD 169          
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Match: UPI000B8C9D03 (protein MON2 homolog n=1 Tax=Carica papaya TaxID=3649 RepID=UPI000B8C9D03)

HSP 1 Score: 71.6 bits (174), Expect = 2.250e-11
Identity = 62/171 (36.26%), Postives = 94/171 (54.97%), Query Frame = 1
Query:   13 LRALESDLRALAQTARAGHPAVREVAERAIVKLRSIP-PDAPFPPAETIRPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVLSRQSEHPSETIRVRALQTLPLVL-SRSPPGSVGVSEIAAAMSLGLSMMRGD-SSGLLRGMAAATVRQAAGIAFD 516
            + ALESDLRAL+  AR  +PAV++ AE AI+KLRS+  P       + +R  L+AC           L    L  LQ+L + +++ PS    IL  L   +E   E+++++ LQT+ ++  SR  P S   S +A A+ + L ++  + SS  +R  AAAT RQA  + FD
Sbjct:    4 MAALESDLRALSAEARRRYPAVKDGAEHAILKLRSLSNPSETSNNEDILRIFLMACE-----VRTVKLSVIGLSCLQKLISHDAVDPSALKEILSTLKDHAEMADESVQLKTLQTILIIFQSRLHPESE--SNMAQALGICLRLLDNNRSSDSVRNTAAATFRQAVALIFD 167          
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Match: A0A1R3GWA4_COCAP (Uncharacterized protein n=2 Tax=Corchorus TaxID=93758 RepID=A0A1R3GWA4_COCAP)

HSP 1 Score: 71.6 bits (174), Expect = 2.250e-11
Identity = 60/168 (35.71%), Postives = 91/168 (54.17%), Query Frame = 1
Query:   22 LESDLRALAQTARAGHPAVREVAERAIVKLRSIP-PDAPFPPAETIRPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVLSRQSEHPSETIRVRALQTLPLVL-SRSPPGSVGVSEIAAAMSLGLSMMRGD-SSGLLRGMAAATVRQAAGIAFD 516
            LESDLRAL+  AR  +PAV++ AE AI+KLR++  P       + +R  L+AC           L    L  LQ+L + +++ PS    IL  L   +E   ET++++ LQT+ ++  SR  P S     +A A+ + L ++  + SS  +R  AAAT RQA  + FD
Sbjct:    7 LESDLRALSFEARRRYPAVKDAAEHAILKLRTLSSPSEILHHEDIVRIFLMACE-----VKTVKLSVIGLSCLQKLISHDAVAPSVLNDILPTLKEHAEMADETVQLKTLQTILIIFQSRLHPESE--ENMAQALGICLRLLENNKSSDSVRNTAAATFRQAVALVFD 167          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig84909.19790.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A6J1HLJ3_CUCMA1.930e-1238.10protein MON2 homolog isoform X1 n=16 Tax=Cucurbita... [more]
UPI000C1D6B284.850e-1235.06protein MON2 homolog isoform X1 n=2 Tax=Olea europ... [more]
A0A1U8N3B0_GOSHI1.220e-1136.31protein MON2 homolog n=21 Tax=Gossypium TaxID=3633... [more]
A0A0A9LWA6_ARUDO1.580e-1135.29Protein MON2 homolog n=1 Tax=Arundo donax TaxID=35... [more]
S8CYZ5_9LAMI1.640e-1135.93Uncharacterized protein (Fragment) n=1 Tax=Genlise... [more]
A0A5N6QSQ2_9ROSI1.660e-1136.53Uncharacterized protein n=2 Tax=Carpinus fangiana ... [more]
A0A446Q1V0_TRITD1.670e-1134.71DCB domain-containing protein n=2 Tax=Triticum tur... [more]
A0A388KWM5_CHABU1.670e-1133.53Uncharacterized protein n=1 Tax=Chara braunii TaxI... [more]
UPI000B8C9D032.250e-1136.26protein MON2 homolog n=1 Tax=Carica papaya TaxID=3... [more]
A0A1R3GWA4_COCAP2.250e-1135.71Uncharacterized protein n=2 Tax=Corchorus TaxID=93... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig84909contigM-pyrifera_M_contig84909:242..871 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score88.6
Seed ortholog evalue3.1e-15
Seed eggNOG ortholog2880.D8LQH8
KEGG koko:K18466
KEGG Pathwayko04144,map04144
Hectar predicted targeting categoryother localisation
EggNOG free text desc.protein transport
EggNOG OGsKOG1848@1,KOG1848@2759
COG Functional cat.KLT
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko04131
Exons2
Model size516
Cds size516
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816756.6017354-CDS-M-pyrifera_M_contig84909:241..5501622816756.6017354-CDS-M-pyrifera_M_contig84909:241..550Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84909 242..550 +
1692278159.8874495-CDS-M-pyrifera_M_contig84909:241..5501692278159.8874495-CDS-M-pyrifera_M_contig84909:241..550Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84909 242..550 +
1622816756.6145575-CDS-M-pyrifera_M_contig84909:664..8711622816756.6145575-CDS-M-pyrifera_M_contig84909:664..871Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84909 665..871 +
1692278159.9026315-CDS-M-pyrifera_M_contig84909:664..8711692278159.9026315-CDS-M-pyrifera_M_contig84909:664..871Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84909 665..871 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig84909.19790.1prot_M-pyrifera_M_contig84909.19790.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig84909 242..871 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig84909.19790.1

>prot_M-pyrifera_M_contig84909.19790.1 ID=prot_M-pyrifera_M_contig84909.19790.1|Name=mRNA_M-pyrifera_M_contig84909.19790.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=172bp
MTTPLRALESDLRALAQTARAGHPAVREVAERAIVKLRSIPPDAPFPPAE
TIRPLLLACHHVSGGSNEEALVTAALGSLQRLATTESIPPSEPGSILQVL
SRQSEHPSETIRVRALQTLPLVLSRSPPGSVGVSEIAAAMSLGLSMMRGD
SSGLLRGMAAATVRQAAGIAFD
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mRNA from alignment at M-pyrifera_M_contig84909:242..871+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig84909.19790.1 ID=mRNA_M-pyrifera_M_contig84909.19790.1|Name=mRNA_M-pyrifera_M_contig84909.19790.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=630bp|location=Sequence derived from alignment at M-pyrifera_M_contig84909:242..871+ (Macrocystis pyrifera P11B4 male)
ATGACGACGCCATTGCGTGCGTTGGAAAGTGACCTACGGGCACTAGCACA AACAGCTCGTGCTGGACATCCTGCTGTGAGAGAGGTAGCAGAACGAGCAA TTGTGAAATTGCGTTCCATCCCTCCAGACGCACCGTTCCCTCCCGCAGAA ACCATTCGGCCACTCCTTTTGGCTTGCCATCATGTTAGTGGGGGTTCAAA TGAGGAAGCATTGGTTACTGCTGCTCTTGGTTCGCTTCAGAGGCTTGCAA CAACAGAGAGCATTCCTCCGTCTGAGCCAGGTAGTATCTTACAAGTGTTA TCAAGACAGGTATGTACTCAAAGGTTGGGGGATTGGGTGGGGGAGGGGGT GTAGGGTGGTGATTGTGGCGGGGATGTAGGAGACTGGGTGATTGGGGGAT GTTGGAGGATTTTTTTGTTGCAGTCGGAGCATCCAAGTGAAACGATTCGT GTGCGTGCATTACAAACATTACCACTAGTTTTGTCTCGTAGTCCTCCTGG TAGTGTAGGGGTGAGTGAGATTGCAGCAGCCATGTCACTAGGTCTTTCGA TGATGCGTGGGGATTCTAGTGGGTTGTTGCGTGGAATGGCAGCAGCAACA GTACGTCAAGCAGCTGGAATTGCATTTGAT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig84909:242..871+

>mRNA_M-pyrifera_M_contig84909.19790.1 ID=mRNA_M-pyrifera_M_contig84909.19790.1|Name=mRNA_M-pyrifera_M_contig84909.19790.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1032bp|location=Sequence derived from alignment at M-pyrifera_M_contig84909:242..871+ (Macrocystis pyrifera P11B4 male)
ATGACGACGCCATTGCGTGCGTTGGAAAGTGACCTACGGGCACTAGCACA
AACAGCTCGTGCTGGACATCCTGCTGTGAGAGAGGTAGCAGAACGAGCAA
TTGTGAAATTGCGTTCCATCCCTCCAGACGCACCGTTCCCTCCCGCAGAA
ACCATTCGGCCACTCCTTTTGGCTTGCCATCATGTTAGTGGGGGTTCAAA
TGAGGAAGCATTGGTTACTGCTGCTCTTGGTTCGCTTCAGAGGCTTGCAA
CAACAGAGAGCATTCCTCCGTCTGAGCCAGGTAGTATCTTACAAGTGTTA
TCAAGACAGATGACGACGCCATTGCGTGCGTTGGAAAGTGACCTACGGGC
ACTAGCACAAACAGCTCGTGCTGGACATCCTGCTGTGAGAGAGGTAGCAG
AACGAGCAATTGTGAAATTGCGTTCCATCCCTCCAGACGCACCGTTCCCT
CCCGCAGAAACCATTCGGCCACTCCTTTTGGCTTGCCATCATGTTAGTGG
GGGTTCAAATGAGGAAGCATTGGTTACTGCTGCTCTTGGTTCGCTTCAGA
GGCTTGCAACAACAGAGAGCATTCCTCCGTCTGAGCCAGGTAGTATCTTA
CAAGTGTTATCAAGACAGTCGGAGCATCCAAGTGAAACGATTCGTGTGCG
TGCATTACAAACATTACCACTAGTTTTGTCTCGTAGTCCTCCTGGTAGTG
TAGGGGTGAGTGAGATTGCAGCAGCCATGTCACTAGGTCTTTCGATGATG
CGTGGGGATTCTAGTGGGTTGTTGCGTGGAATGGCAGCAGCAACAGTACG
TCAAGCAGCTGGAATTGCATTTGATTCGGAGCATCCAAGTGAAACGATTC
GTGTGCGTGCATTACAAACATTACCACTAGTTTTGTCTCGTAGTCCTCCT
GGTAGTGTAGGGGTGAGTGAGATTGCAGCAGCCATGTCACTAGGTCTTTC
GATGATGCGTGGGGATTCTAGTGGGTTGTTGCGTGGAATGGCAGCAGCAA
CAGTACGTCAAGCAGCTGGAATTGCATTTGAT
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