mRNA_M-pyrifera_M_contig84821.19760.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig84821.19760.1
Unique NamemRNA_M-pyrifera_M_contig84821.19760.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Match: A0A8J4PWQ0_9MYCE (Uncharacterized protein n=1 Tax=Polysphondylium violaceum TaxID=133409 RepID=A0A8J4PWQ0_9MYCE)

HSP 1 Score: 63.5 bits (153), Expect = 7.890e-9
Identity = 48/170 (28.24%), Postives = 78/170 (45.88%), Query Frame = 1
Query:   22 FAVCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVA-----VFVTNGTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPMAS---HGRGKRTLVVAKSREVAGRLADGYGWSDWEFLDDYAARHPWLLPDKSRH------APSGXXXXXXXDHRPVEAVMVVQIPTDW 489
            F + FD+DGV+ + G      +  A  A+  + D          +FVTNG G +EA+KA  +S++L   I+    ++  TPM +     R    L+V+KS + +  LA  YG+  +  L  Y    P+L P K         A          D  P +A+++++ P DW
Sbjct:   11 FGIVFDIDGVLIRDGV----PIPNATKALGLLVDQDTQQPKYPYIFVTNGGGFSEADKAKKVSKVLNHHIEEDHCMVAHTPMKTLVDKYRDNDVLLVSKSHKTSEHLAQVYGFKHFTSLQQYVEERPFLYPTKYSTFWTKGVASEYDIKKSKDDQLPFKAIILLEEPLDW 176          
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Match: A0A8K1CB61_PYTOL (Uncharacterized protein n=1 Tax=Pythium oligandrum TaxID=41045 RepID=A0A8K1CB61_PYTOL)

HSP 1 Score: 62.0 bits (149), Expect = 2.730e-8
Identity = 51/167 (30.54%), Postives = 79/167 (47.31%), Query Frame = 1
Query:    7 ASRARFAVCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVAVFVTNGTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPM---ASHGRGKRTLVVAKSREVAGRLADGYGWSDWEFLDDYAARHPWLLPDKS---RHAPSGXXXXXXXDHRPVEAVMVVQIPTDW 489
            AS   F + FD+DGV+ + G     E+  A   + ++ +     +F+TNG G  E EKA  L R L +EID   ++L  TPM       +  R L++  S +V   +A  YG+S    + + A  HP   P      +HAP            P+EA++++  P DW
Sbjct:   30 ASEPSFGIAFDIDGVLVRGG----KELPRARQVLLSLRENNVPHIFLTNGGGCMEEEKAKNLGRALDLEIDPKQMILSHTPMRELVEKYKDARVLIMG-SYDVY-TVAREYGFSKVVSVKELAHHHPTQYPFNKYDHKHAPH--------HEEPIEAIIIMHDPIDW 182          
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Match: A0A197K6P7_9FUNG (HAD-superfamily hydrolase n=1 Tax=Linnemannia elongata AG-77 TaxID=1314771 RepID=A0A197K6P7_9FUNG)

HSP 1 Score: 62.0 bits (149), Expect = 2.900e-8
Identity = 49/162 (30.25%), Postives = 74/162 (45.68%), Query Frame = 1
Query:   13 RARFAVCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVAVFVTNGTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPM---ASHGRGKRTLVVAKSREVAGRLADGYGWSDWEFLDDYAARHPWLLPDKSRHAPSGXXXXXXXDHRPVEAVMVVQIPTDW 489
            +A + V FD+DGV+ K    G   +     A+  +       +F+TNG G  EA+KA  LS+ +G+ I    ++L  +PM   A        LVV  +      +A+ YG+      ++  A  P + P  +R A S         HRPVEA+MV     DW
Sbjct:   42 QAHYNVVFDIDGVLIK----GKQVLPQTHRALGLLQSNNVPYIFLTNGGGLKEADKAEQLSKKIGVHISPKQLILSHSPMRELAQKYEDSNVLVVGGNGSECRHVAEHYGFKHIVTPEEVHAVFPSVCPSSAREARSKYLQNV---HRPVEAIMVFHDSVDW 196          
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Match: Q4D3X0_TRYCC (Uncharacterized protein n=7 Tax=Trypanosoma cruzi TaxID=5693 RepID=Q4D3X0_TRYCC)

HSP 1 Score: 62.0 bits (149), Expect = 3.260e-8
Identity = 43/126 (34.13%), Postives = 66/126 (52.38%), Query Frame = 1
Query:   28 VCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVAVFVTNGTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPM---ASHGRGKRTLVVAKSREVAGRLADGYGWSDWEFLDDYAARHPWLLPDK 396
            +  D+DGVVY+        ++G+  A++ + +     +F+TNG G +E EKA  LS+L+G EID+  +LL  TPM   A   + +  LVV         +A  YG+     +  + A HP LLP K
Sbjct:  171 IVLDIDGVVYRS----HKLIEGSDTAIRKMTELRIPLLFMTNGGGISEEEKARELSQLVGCEIDSSQILLAHTPMQLLAPMYKNQNVLVVGNPHSA--EVAKMYGFDHAISVLQFQAEHPELLPYK 290          
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Match: A0A1X0P5B2_9TRYP (Uncharacterized protein n=1 Tax=Trypanosoma theileri TaxID=67003 RepID=A0A1X0P5B2_9TRYP)

HSP 1 Score: 61.6 bits (148), Expect = 4.440e-8
Identity = 45/126 (35.71%), Postives = 65/126 (51.59%), Query Frame = 1
Query:   28 VCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVAVFVTNGTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPM---ASHGRGKRTLVVAKSREVAGRLADGYGWSDWEFLDDYAARHPWLLPDK 396
            +  DLDGVV++        ++G+  A++ +       +F+TNG G TEAEKA  LS L+G EI    VLL  TPM   A   + +  LVV   + V   +A  YG+S    +  +   HP L+P K
Sbjct:  176 IVLDLDGVVHRS----NKIIEGSDTAIRKIMQLRIPLLFMTNGGGVTEAEKAKQLSELVGCEISPDQVLLSHTPMQLLAPIYKNQNILVVGSPKCVD--VAKSYGFSRPISMLQFQVEHPELVPYK 295          
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Match: A0A2D4BVA6_PYTIN (Uncharacterized protein n=1 Tax=Pythium insidiosum TaxID=114742 RepID=A0A2D4BVA6_PYTIN)

HSP 1 Score: 60.1 bits (144), Expect = 1.280e-7
Identity = 48/167 (28.74%), Postives = 80/167 (47.90%), Query Frame = 1
Query:    1 AMASRARFAVCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVAVFVTNGTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPM---ASHGRGKRTLVVAKSREVAGRLADGYGWSDWEFLDDYAARHPWLLP-DKSRHAPSGXXXXXXXDHRPVEAVMVVQIPTDW 489
            A  +   F + FD+DGV+ + G     E+  A   +  + +     +F+TNG G  EAEKA  L R L ++I+   ++L  TPM       +  R L++  S +V   +A  YG++    ++D   +HP   P  K  H P+           P+EA++++  P DW
Sbjct:   26 ASTNTPSFGIAFDIDGVLVRGG----KELPRARDIILRLREDNVPHIFLTNGGGCMEAEKAANLGRALDLDIEPRQMVLSHTPMRELVEKFKDSRVLIMG-SYDVY-NVAREYGFNKVVSVNDLVQQHPTQYPFHKYEHKPAPFHG------EPIEAIIIMHDPIDW 180          
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Match: M4B8B3_HYAAE (Uncharacterized protein n=1 Tax=Hyaloperonospora arabidopsidis (strain Emoy2) TaxID=559515 RepID=M4B8B3_HYAAE)

HSP 1 Score: 59.3 bits (142), Expect = 1.450e-7
Identity = 48/167 (28.74%), Postives = 80/167 (47.90%), Query Frame = 1
Query:    4 MASRARFAVCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVAVFVTNGTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPMASHGR--GKRTLVVAKSREVAGRLADGYGWSDWEFLDDYAARHPWLLPD---KSRHAPSGXXXXXXXDHRPVEAVMVVQIPTDW 489
            + S   F + FD+DGV+ + G      +  A   +K++       +F+TNG G  E EKA  LS +L + ID   ++L  TPM    +  G + +++  S +V  R+A  YG++    + D    HP   P    + R AP          + P+EA++V+  P +W
Sbjct:   25 LRSAPSFGIAFDIDGVLIRGG----HALPKAKRVLKSLRSNNVAHIFLTNGGGCMEDEKAKNLSTILDVPIDPEHMILSHTPMREIAKTYGDKRVLIMGSHDVW-RVAKCYGFNKIVSVKDLLHHHPMQYPFMHLEQRAAPHY--------NEPIEAIIVMHDPIEW 178          
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Match: UPI0004F3F07F (HAD-superfamily subfamily IIA hydrolase n=1 Tax=Trypanosoma grayi TaxID=71804 RepID=UPI0004F3F07F)

HSP 1 Score: 59.7 bits (143), Expect = 2.060e-7
Identity = 41/126 (32.54%), Postives = 68/126 (53.97%), Query Frame = 1
Query:   28 VCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVAVFVTNGTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPM---ASHGRGKRTLVVAKSREVAGRLADGYGWSDWEFLDDYAARHPWLLPDK 396
            +  D+DGVVY+        ++G+ AA++ + +     +F+TNG G +E EKA  LS+L+G +ID   +LL  +PM   A   + +  LVV + R     +A  YG++    +  Y   HP ++P K
Sbjct:  176 IVLDIDGVVYRS----HKLIEGSDAAIRKIMELHIPLLFMTNGGGLSEEEKAEELSQLVGCKIDDSQILLAHSPMKLLAPLYKNQNVLVVGRPR--CADVAKMYGFNRAMSILQYQVEHPDMVPYK 295          
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Match: H3HC72_PHYRM (Uncharacterized protein n=1 Tax=Phytophthora ramorum TaxID=164328 RepID=H3HC72_PHYRM)

HSP 1 Score: 59.3 bits (142), Expect = 2.300e-7
Identity = 48/166 (28.92%), Postives = 78/166 (46.99%), Query Frame = 1
Query:    7 ASRARFAVCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVAVFVTNGTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPMASHGR--GKRTLVVAKSREVAGRLADGYGWSDWEFLDDYAARHPW---LLPDKSRHAPSGXXXXXXXDHRPVEAVMVVQIPTDW 489
            AS   F + FD+DGV+ + G     E+  A   ++++       +F+TNG G  E EKA  LS +L + I+   ++L  TPM    +  G + +++  S +V   +A  YG+     + D    HP     LP +   AP            P+EA++V+  P DW
Sbjct:   14 ASAPSFGIAFDIDGVLIRGG----HELPKAKRVLQSLRANNVPHIFLTNGGGCMEEEKANKLSDVLDLTINPDHMILSHTPMREIAKTYGDKRVLIMGSHDVW-NVAKCYGFKKVVSVQDLLHHHPLQYPFLPYEQTPAPH--------HEEPIEAIIVMHDPIDW 166          
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Match: A0A1V6Y0U1_PENNA (Uncharacterized protein n=5 Tax=Penicillium TaxID=5073 RepID=A0A1V6Y0U1_PENNA)

HSP 1 Score: 59.3 bits (142), Expect = 2.590e-7
Identity = 40/113 (35.40%), Postives = 58/113 (51.33%), Query Frame = 1
Query:    7 ASRARFAVCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVAVFVTNGTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPMASHGRGKRT-LVVAKSREVAGRLADGYGWSD 342
            +S  RFA+ FD+DGV+ K G      +  ++ A+  +       +FVTNG G+TE E+   LSR L ME+  G  +   TPM       +T LVV    E    +A+ YG+ D
Sbjct:   72 SSTDRFAIAFDIDGVLLKGG----KPIPESIGAMNYINGDNPPYIFVTNGGGKTEEERCIDLSRQLQMEVSPGQFICGHTPMREMAEAYQTVLVVGGEGEKCRVVAEDYGFKD 180          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig84821.19760.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A8J4PWQ0_9MYCE7.890e-928.24Uncharacterized protein n=1 Tax=Polysphondylium vi... [more]
A0A8K1CB61_PYTOL2.730e-830.54Uncharacterized protein n=1 Tax=Pythium oligandrum... [more]
A0A197K6P7_9FUNG2.900e-830.25HAD-superfamily hydrolase n=1 Tax=Linnemannia elon... [more]
Q4D3X0_TRYCC3.260e-834.13Uncharacterized protein n=7 Tax=Trypanosoma cruzi ... [more]
A0A1X0P5B2_9TRYP4.440e-835.71Uncharacterized protein n=1 Tax=Trypanosoma theile... [more]
A0A2D4BVA6_PYTIN1.280e-728.74Uncharacterized protein n=1 Tax=Pythium insidiosum... [more]
M4B8B3_HYAAE1.450e-728.74Uncharacterized protein n=1 Tax=Hyaloperonospora a... [more]
UPI0004F3F07F2.060e-732.54HAD-superfamily subfamily IIA hydrolase n=1 Tax=Tr... [more]
H3HC72_PHYRM2.300e-728.92Uncharacterized protein n=1 Tax=Phytophthora ramor... [more]
A0A1V6Y0U1_PENNA2.590e-735.40Uncharacterized protein n=5 Tax=Penicillium TaxID=... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig84821contigM-pyrifera_M_contig84821:207..695 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score74.7
Seed ortholog evalue4.4e-11
Seed eggNOG ortholog5762.XP_002682271.1
KEGG rclassRC00055,RC00523
KEGG koko:K22503
KEGG ReactionR05577
KEGG Pathwayko00970,map00970
KEGG ModuleM00359
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0006629,GO:0006644,GO:0006650,GO:0006655,GO:0006793,GO:0006796,GO:0006996,GO:0007005,GO:0007006,GO:0008150,GO:0008152,GO:0008610,GO:0008654,GO:0008808,GO:0009058,GO:0009987,GO:0016043,GO:0016740,GO:0016772,GO:0016780,GO:0016787,GO:0019637,GO:0030572,GO:0032048,GO:0032049,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044238,GO:0044249,GO:0044255,GO:0044424,GO:0044444,GO:0044464,GO:0045017,GO:0046471,GO:0046474,GO:0046486,GO:0061024,GO:0071704,GO:0071840,GO:0090407,GO:1901576
EggNOG free text desc.glycerophospholipid biosynthetic process
EggNOG OGsCOG0647@1,KOG1618@2759
EC6.1.1.12
COG Functional cat.G
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko01007,ko03016
Exons1
Model size489
Cds size486
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816754.9888306-CDS-M-pyrifera_M_contig84821:206..6921622816754.9888306-CDS-M-pyrifera_M_contig84821:206..692Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84821 207..692 -
1692278158.885121-CDS-M-pyrifera_M_contig84821:206..6921692278158.885121-CDS-M-pyrifera_M_contig84821:206..692Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig84821 207..692 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816754.999394-UTR-M-pyrifera_M_contig84821:692..6951622816754.999394-UTR-M-pyrifera_M_contig84821:692..695Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig84821 693..695 -
1692278158.9058523-UTR-M-pyrifera_M_contig84821:692..6951692278158.9058523-UTR-M-pyrifera_M_contig84821:692..695Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig84821 693..695 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig84821.19760.1prot_M-pyrifera_M_contig84821.19760.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig84821 207..692 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig84821.19760.1

>prot_M-pyrifera_M_contig84821.19760.1 ID=prot_M-pyrifera_M_contig84821.19760.1|Name=mRNA_M-pyrifera_M_contig84821.19760.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=162bp
MASRARFAVCFDLDGVVYKHGPGGTSEVDGAVAAVKAVCDAGAVAVFVTN
GTGQTEAEKAGMLSRLLGMEIDAGSVLLPTTPMASHGRGKRTLVVAKSRE
VAGRLADGYGWSDWEFLDDYAARHPWLLPDKSRHAPSGGGGGGGDDHRPV
EAVMVVQIPTDW
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mRNA from alignment at M-pyrifera_M_contig84821:207..695-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig84821.19760.1 ID=mRNA_M-pyrifera_M_contig84821.19760.1|Name=mRNA_M-pyrifera_M_contig84821.19760.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=489bp|location=Sequence derived from alignment at M-pyrifera_M_contig84821:207..695- (Macrocystis pyrifera P11B4 male)
GCAATGGCCAGCCGTGCTCGCTTCGCAGTGTGTTTCGACCTGGATGGCGT GGTGTACAAGCACGGACCTGGTGGCACGTCGGAGGTGGACGGGGCGGTGG CGGCGGTGAAGGCGGTGTGTGACGCTGGAGCTGTTGCGGTGTTCGTCACG AATGGGACCGGGCAGACGGAGGCGGAGAAGGCGGGCATGCTCTCCCGGCT TCTCGGCATGGAGATTGATGCTGGATCTGTGTTGTTGCCCACCACGCCAA TGGCATCTCACGGGCGCGGAAAGCGCACGCTGGTCGTCGCAAAGTCGCGC GAGGTCGCCGGGCGATTGGCAGATGGATACGGCTGGTCCGACTGGGAGTT CCTGGACGACTACGCCGCCCGCCATCCTTGGCTGCTGCCAGACAAGTCCC GTCACGCGCCATCTGGCGGTGGTGGCGGCGGAGGGGATGACCACCGCCCC GTGGAGGCGGTCATGGTTGTGCAGATCCCCACCGACTGG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig84821:207..695-

>mRNA_M-pyrifera_M_contig84821.19760.1 ID=mRNA_M-pyrifera_M_contig84821.19760.1|Name=mRNA_M-pyrifera_M_contig84821.19760.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=972bp|location=Sequence derived from alignment at M-pyrifera_M_contig84821:207..695- (Macrocystis pyrifera P11B4 male)
ATGGCCAGCCGTGCTCGCTTCGCAGTGTGTTTCGACCTGGATGGCGTGGT
GTACAAGCACGGACCTGGTGGCACGTCGGAGGTGGACGGGGCGGTGGCGG
CGGTGAAGGCGGTGTGTGACGCTGGAGCTGTTGCGGTGTTCGTCACGAAT
GGGACCGGGCAGACGGAGGCGGAGAAGGCGGGCATGCTCTCCCGGCTTCT
CGGCATGGAGATTGATGCTGGATCTGTGTTGTTGCCCACCACGCCAATGG
CATCTCACGGGCGCGGAAAGCGCACGCTGGTCGTCGCAAAGTCGCGCGAG
GTCGCCGGGCGATTGGCAGATGGATACGGCTGGTCCGACTGGGAGTTCCT
GGACGACTACGCCGCCCGCCATCCTTGGCTGCTGCCAGACAAGTCCCGTC
ACGCGCCATCTGGCGGTGGTGGCGGCGGAGGGGATGACCACCGCCCCGTG
GAGGCGGTCATGGTTGTGCAGATCCCCACCGACTGGATGGCCAGCCGTGC
TCGCTTCGCAGTGTGTTTCGACCTGGATGGCGTGGTGTACAAGCACGGAC
CTGGTGGCACGTCGGAGGTGGACGGGGCGGTGGCGGCGGTGAAGGCGGTG
TGTGACGCTGGAGCTGTTGCGGTGTTCGTCACGAATGGGACCGGGCAGAC
GGAGGCGGAGAAGGCGGGCATGCTCTCCCGGCTTCTCGGCATGGAGATTG
ATGCTGGATCTGTGTTGTTGCCCACCACGCCAATGGCATCTCACGGGCGC
GGAAAGCGCACGCTGGTCGTCGCAAAGTCGCGCGAGGTCGCCGGGCGATT
GGCAGATGGATACGGCTGGTCCGACTGGGAGTTCCTGGACGACTACGCCG
CCCGCCATCCTTGGCTGCTGCCAGACAAGTCCCGTCACGCGCCATCTGGC
GGTGGTGGCGGCGGAGGGGATGACCACCGCCCCGTGGAGGCGGTCATGGT
TGTGCAGATCCCCACCGACTGG
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