mRNA_M-pyrifera_M_contig109412.1973.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig109412.1973.1
Unique NamemRNA_M-pyrifera_M_contig109412.1973.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Match: A0A7V3KNJ7_9BACT (Uncharacterized protein n=1 Tax=candidate division WOR-3 bacterium TaxID=2052148 RepID=A0A7V3KNJ7_9BACT)

HSP 1 Score: 201 bits (511), Expect = 6.910e-61
Identity = 98/178 (55.06%), Postives = 129/178 (72.47%), Query Frame = 1 
Query:    1 VTYKPKRIAAPRRPYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFTTHVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKALLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTIDGSAVSDLKNAFEMRHPITHNLGVIDRK 534
            ++Y P R     RP EE +RRD+ICP+C L+HAVFGLATWCPDCG+DIF  HVEAEL VVR +L   + RR +LGAR+ A+D+EN LEDVVS++EAVLK +  RYL + G+   E+ ++++K VRN +QNVE +        G ELF+ I  S + +LK+ FE RHPITHNLG++DRK
Sbjct:  127 MSYTPSRPNIVSRPLEEELRRDIICPSCGLEHAVFGLATWCPDCGTDIFLVHVEAELAVVRKILGAVDSRRSDLGARVAARDIENALEDVVSVFEAVLKVITKRYLLKNGVSVVEITRILNKVVRNSYQNVETAADTFRAQLGMELFEGISDSDIVELKSIFEKRHPITHNLGIVDRK 304
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BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Match: A0A2N2JZP0_9DELT (Uncharacterized protein n=1 Tax=Deltaproteobacteria bacterium HGW-Deltaproteobacteria-15 TaxID=2013742 RepID=A0A2N2JZP0_9DELT)

HSP 1 Score: 201 bits (510), Expect = 1.090e-60
Identity = 97/178 (54.49%), Postives = 126/178 (70.79%), Query Frame = 1 
Query:    1 VTYKPKRIAAPRRPYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFTTHVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKALLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTIDGSAVSDLKNAFEMRHPITHNLGVIDRK 534
            ++ KP +     RP+EE +RRDV+CP C LDH+V+GLATWC DCG DIF THVE+EL V+R ML D ERR E LG R+ AKD+ENCLED VSI+EAVLK L+ RY ++ G   +E+++L  KK+ N FQN+ RS  +     G +LFD++    V  L   FE RHPITHNLG++D+K
Sbjct:  126 MSLKPGQKPVIHRPFEEKIRRDVVCPRCGLDHSVYGLATWCADCGEDIFLTHVESELSVIRTMLGDVERRMETLGVRVAAKDMENCLEDTVSIFEAVLKTLVRRYKRKNGTPVEEIDRLF-KKIGNSFQNIVRSVDVFENDVGMKLFDSLSEETVDALARVFEKRHPITHNLGIVDKK 302
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BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Match: A0A437RK79_9BURK (Uncharacterized protein n=1 Tax=Aquincola rivuli TaxID=1862385 RepID=A0A437RK79_9BURK)

HSP 1 Score: 196 bits (497), Expect = 1.150e-58
Identity = 98/178 (55.06%), Postives = 129/178 (72.47%), Query Frame = 1 
Query:    1 VTYKPKRIAAPRRPYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFTTHVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKALLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTIDGSAVSDLKNAFEMRHPITHNLGVIDRK 534
            +TYKP  + + RRPYE+ VRRDV+CP+C LDH VFGLATWC DCG DIF THV+AEL V R+M+ D ERRRE LG R+ AKD+ENCLED VS++EA +KAL  R L   G   +EVE  + +K+ NG+QN+ R++ ++ +  G +L   ++   + +   AFE RHPITHNLGV+DRK
Sbjct:  126 MTYKPGALPSVRRPYEDEVRRDVVCPHCALDHTVFGLATWCSDCGEDIFLTHVQAELAVTRSMVGDMERRRETLGPRVAAKDLENCLEDAVSLFEAAMKALSRRRLASTGASEEEVEARL-RKLGNGYQNIARTKELLPDVLGIDLPPGVEWDRLGE---AFEKRHPITHNLGVVDRK 299
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BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Match: A0A7Y4S8H1_9PROT (Uncharacterized protein n=1 Tax=Methylotenera sp. TaxID=2051956 RepID=A0A7Y4S8H1_9PROT)

HSP 1 Score: 190 bits (482), Expect = 1.760e-56
Identity = 97/178 (54.49%), Postives = 128/178 (71.91%), Query Frame = 1 
Query:    1 VTYKPKRIAAPRRPYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFTTHVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKALLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTIDGSAVSDLKNAFEMRHPITHNLGVIDRK 534
            ++YKP  +    RP+E+ VRRDVICP+CTLD  VFGLATWC DCG DIF TH+EAEL V R ML D ERR+++LG R+ AKD+ENCLEDVVSIYEA ++A++ R +  +G  S+ +E    KK+ N FQN++R++  +    G    + ++   +  LK+AFE RHPITHNLGVIDRK
Sbjct:  125 ISYKPGSLPYVHRPFEDEVRRDVICPHCTLDQTVFGLATWCADCGEDIFMTHIEAELAVTRLMLEDVERRQDQLGKRVAAKDLENCLEDVVSIYEAAIRAIVRRAMIIRGDSSELIETTF-KKLGNAFQNIDRTQKELNSLFGK---NALNEDVLEKLKSAFEKRHPITHNLGVIDRK 298
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BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Match: A0A1M3KBE9_9CHLR (Uncharacterized protein n=2 Tax=unclassified Chloroflexi TaxID=189774 RepID=A0A1M3KBE9_9CHLR)

HSP 1 Score: 187 bits (475), Expect = 1.730e-55
Identity = 92/178 (51.69%), Postives = 121/178 (67.98%), Query Frame = 1 
Query:    1 VTYKPKRIAAPRRPYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFTTHVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKALLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTIDGSAVSDLKNAFEMRHPITHNLGVIDRK 534
            +TYK  +     RP EE +RRD+ICPNC L+HAVFGLATWCPDCG DIF  HVE E  V++ +LS  ++RREELGAR+  +D+EN LEDVVS++EAVLK +  ++L   GM  DE+ K + K +RN +QN+  +       TG +LF       V  L+  FE RHP+THNLGV+D+K
Sbjct:  126 MTYKSSKPDYVPRPLEEELRRDIICPNCGLEHAVFGLATWCPDCGKDIFMCHVETEFGVIQKVLSVVDKRREELGARVAGRDIENGLEDVVSVFEAVLKIITIKHLANNGMSHDEISKRLEK-IRNSYQNINSAGETFHAQTGLDLFHNTSQDEVETLRTIFEKRHPLTHNLGVVDKK 302
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BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Match: UPI00037F14B3 (hypothetical protein n=1 Tax=Methylotenera mobilis TaxID=359408 RepID=UPI00037F14B3)

HSP 1 Score: 182 bits (461), Expect = 2.070e-53
Identity = 91/178 (51.12%), Postives = 126/178 (70.79%), Query Frame = 1 
Query:    1 VTYKPKRIAAPRRPYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFTTHVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKALLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTIDGSAVSDLKNAFEMRHPITHNLGVIDRK 534
            +TYKP  +    RP+E+ VRRD++CP+CTLD  VFGLATWC DCG DIF +H+EAEL VV+ ML+D +RR+ + G RI AKD+ENCLED VSI+EA ++A++ R +  +   +D++E    KK+ N FQ+++R++  +    G    + +D      LK+AFE RHPITHNLGVIDRK
Sbjct:  125 MTYKPAALPYVHRPFEDEVRRDIVCPHCTLDQTVFGLATWCADCGEDIFLSHIEAELAVVQLMLNDVDRRQTQFGKRIAAKDLENCLEDTVSIFEAAMRAIVRRAMLMRRDSADQIESSF-KKLGNAFQSIDRTQKELNSLFGK---NPLDEEVSEKLKSAFEKRHPITHNLGVIDRK 298
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BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Match: A0A644VDD9_9ZZZZ (Uncharacterized protein n=1 Tax=bioreactor metagenome TaxID=1076179 RepID=A0A644VDD9_9ZZZZ)

HSP 1 Score: 182 bits (463), Expect = 2.790e-53
Identity = 87/178 (48.88%), Postives = 122/178 (68.54%), Query Frame = 1 
Query:    1 VTYKPKRIAAPRRPYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFTTHVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKALLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTIDGSAVSDLKNAFEMRHPITHNLGVIDRK 534
            ++YKP  +     P EE +RRD+ CP+C L HAVFGLATWCPDCG DIF  H+  E + +R MLSD +RRRE LGAR+ A+D+EN LEDVVSI+EA ++A+  R +K+ G   D++++ + +K+ N FQNV  +R +  +    +L   +D   +  L+  FE RHPITHNLG++DRK
Sbjct:  158 MSYKPSSLPPVVPPMEEVLRRDITCPHCGLQHAVFGLATWCPDCGRDIFLNHLAKEFEAIRKMLSDVDRRREALGARVAARDIENALEDVVSIFEAAMRAMTIRKMKENGSSDDQIQEKL-RKIGNKFQNVNLAREVCEDEFKVQLLQGLDPKQIDSLRLTFEKRHPITHNLGIVDRK 334
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BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Match: UPI0012DC54BB (hypothetical protein n=1 Tax=Cupriavidus sp. WS TaxID=1312922 RepID=UPI0012DC54BB)

HSP 1 Score: 181 bits (458), Expect = 8.160e-53
Identity = 93/165 (56.36%), Postives = 116/165 (70.30%), Query Frame = 1 
Query:   40 PYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFTTHVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKALLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTIDGSAVSDLKNAFEMRHPITHNLGVIDRK 534
            P EE VRRDV+CP+CTLD  VFGLATWC DCG DIF TH+E EL+VV  M+ D  RR +ELG R+ AKD+ENCLED VSI+EA +KA+  R+L+ KG +S  VE+   K+  N FQNV R++ I+ E  G    D +      +L  AFE RHPITHNLGV+DR+
Sbjct:  139 PLEEEVRRDVVCPHCTLDQTVFGLATWCSDCGQDIFLTHIEGELNVVGCMVGDIGRREQELGRRVAAKDLENCLEDAVSIFEAAMKAMTRRFLEGKGSDSQAVEEYFVKQG-NAFQNVGRTKKILAELVGSAPADNVPWD---ELNAAFEKRHPITHNLGVVDRQ 299
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BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Match: A0A1V0B7A8_9PSED (Uncharacterized protein n=2 Tax=Gammaproteobacteria TaxID=1236 RepID=A0A1V0B7A8_9PSED)

HSP 1 Score: 179 bits (454), Expect = 2.580e-52
Identity = 96/176 (54.55%), Postives = 124/176 (70.45%), Query Frame = 1 
Query:   10 KPKRIAAPRRPYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFTTHVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKALLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTIDGSAVSD-LKNAFEMRHPITHNLGVIDRK 534
            KP +    RRP+E+ VRRDV+CP+CTLD  VFGLA WC DCG DIF THV AE+ V R+ML+D  RR ++LG R+ AKD+ENCLED VS++EA  KAL  R L Q+G +++ VE  + KKV N FQNV+RSR  + +   +E       SA+ D L  +FE RHP+THNLGV+D+K
Sbjct:  130 KPSQPKQVRRPFEDEVRRDVVCPHCTLDQTVFGLAAWCSDCGKDIFLTHVSAEIAVTRSMLNDIGRREQDLGRRVAAKDLENCLEDSVSLFEAAAKALARRALTQRGDDAETVEAKL-KKVGNSFQNVDRSREQLKKLFSYEPVH----SAIWDRLGGSFEKRHPVTHNLGVVDKK 300
[Close]
BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Match: A0A2V7L8V1_9BACT (Uncharacterized protein n=1 Tax=Gemmatimonadetes bacterium TaxID=2026742 RepID=A0A2V7L8V1_9BACT)

HSP 1 Score: 175 bits (443), Expect = 7.610e-51
Identity = 91/176 (51.70%), Postives = 114/176 (64.77%), Query Frame = 1 
Query:    7 YKPKRIAAPRRPYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFTTHVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKALLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTIDGSAVSDLKNAFEMRHPITHNLGVIDRK 534
            Y P +    RRP EE +RRD+ C +C LDHAVFGLA WCPDCG D+F  HVEAE   V  ML +   RRE LG R+ A+D+EN LEDVVSI+EAVLK +  R L+  G  SD++E+++ + VRN +QNV  +     +  G ELF  I       L   FE RHPITHNLGV+DR+
Sbjct:  106 YTPGQRPMVRRPLEEELRRDIRCRHCGLDHAVFGLAIWCPDCGRDVFIQHVEAEFASVSKMLEELPTRRERLGHRVAARDLENSLEDVVSIFEAVLKIITRRNLQTAGKSSDQIEEVVRRNVRNSYQNVASAENTYRDLVGAELFGGISQQEKEHLSAIFEKRHPITHNLGVVDRR 281
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig109412.1973.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 19
Match NameE-valueIdentityDescription
A0A7V3KNJ7_9BACT6.910e-6155.06Uncharacterized protein n=1 Tax=candidate division... [more]
A0A2N2JZP0_9DELT1.090e-6054.49Uncharacterized protein n=1 Tax=Deltaproteobacteri... [more]
A0A437RK79_9BURK1.150e-5855.06Uncharacterized protein n=1 Tax=Aquincola rivuli T... [more]
A0A7Y4S8H1_9PROT1.760e-5654.49Uncharacterized protein n=1 Tax=Methylotenera sp. ... [more]
A0A1M3KBE9_9CHLR1.730e-5551.69Uncharacterized protein n=2 Tax=unclassified Chlor... [more]
UPI00037F14B32.070e-5351.12hypothetical protein n=1 Tax=Methylotenera mobilis... [more]
A0A644VDD9_9ZZZZ2.790e-5348.88Uncharacterized protein n=1 Tax=bioreactor metagen... [more]
UPI0012DC54BB8.160e-5356.36hypothetical protein n=1 Tax=Cupriavidus sp. WS Ta... [more]
A0A1V0B7A8_9PSED2.580e-5254.55Uncharacterized protein n=2 Tax=Gammaproteobacteri... [more]
A0A2V7L8V1_9BACT7.610e-5151.70Uncharacterized protein n=1 Tax=Gemmatimonadetes b... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig109412contigM-pyrifera_M_contig109412:71..604 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score186.0
Seed ortholog evalue1.5e-44
Seed eggNOG ortholog1132855.KB913035_gene143
Hectar predicted targeting categoryother localisation
EggNOG OGs1R47P@1224,28MDK@1,2ZARD@2
Best tax levelProteobacteria
Best eggNOG OGNA|NA|NA
Exons1
Model size534
Cds size534
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622815604.718509-CDS-M-pyrifera_M_contig109412:70..6041622815604.718509-CDS-M-pyrifera_M_contig109412:70..604Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig109412 71..604 +
1692277483.769415-CDS-M-pyrifera_M_contig109412:70..6041692277483.769415-CDS-M-pyrifera_M_contig109412:70..604Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig109412 71..604 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig109412.1973.1prot_M-pyrifera_M_contig109412.1973.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig109412 71..604 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig109412.1973.1

>prot_M-pyrifera_M_contig109412.1973.1 ID=prot_M-pyrifera_M_contig109412.1973.1|Name=mRNA_M-pyrifera_M_contig109412.1973.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=178bp
VTYKPKRIAAPRRPYEETVRRDVICPNCTLDHAVFGLATWCPDCGSDIFT
THVEAELDVVRAMLSDTERRREELGARIEAKDVENCLEDVVSIYEAVLKA
LLGRYLKQKGMESDEVEKLMSKKVRNGFQNVERSRAIILEHTGHELFDTI
DGSAVSDLKNAFEMRHPITHNLGVIDRK
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mRNA from alignment at M-pyrifera_M_contig109412:71..604+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig109412.1973.1 ID=mRNA_M-pyrifera_M_contig109412.1973.1|Name=mRNA_M-pyrifera_M_contig109412.1973.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=534bp|location=Sequence derived from alignment at M-pyrifera_M_contig109412:71..604+ (Macrocystis pyrifera P11B4 male)
GTAACCTATAAGCCCAAGCGAATCGCGGCACCGAGGCGACCTTATGAAGA
GACTGTCCGACGGGACGTAATCTGCCCCAATTGCACCCTGGATCACGCGG
TATTCGGTTTGGCGACTTGGTGTCCAGACTGTGGCTCGGATATCTTCACG
ACGCACGTGGAGGCGGAATTGGATGTCGTTCGGGCCATGCTATCCGACAC
GGAGCGGCGGAGAGAGGAACTGGGAGCCCGAATCGAAGCAAAAGATGTGG
AGAATTGCCTTGAAGACGTTGTTTCGATCTATGAGGCCGTATTGAAGGCG
CTGTTGGGCCGCTACTTGAAGCAAAAAGGGATGGAATCGGACGAAGTCGA
GAAATTGATGTCCAAGAAGGTCCGGAATGGCTTTCAGAATGTGGAACGAT
CTCGCGCGATTATATTGGAGCATACGGGACATGAATTGTTCGACACGATT
GACGGTTCAGCCGTAAGCGACCTGAAGAACGCGTTCGAAATGCGACATCC
AATTACACACAATCTTGGGGTGATTGATCGGAAG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig109412:71..604+

>mRNA_M-pyrifera_M_contig109412.1973.1 ID=mRNA_M-pyrifera_M_contig109412.1973.1|Name=mRNA_M-pyrifera_M_contig109412.1973.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1068bp|location=Sequence derived from alignment at M-pyrifera_M_contig109412:71..604+ (Macrocystis pyrifera P11B4 male)
GTAACCTATAAGCCCAAGCGAATCGCGGCACCGAGGCGACCTTATGAAGA
GACTGTCCGACGGGACGTAATCTGCCCCAATTGCACCCTGGATCACGCGG
TATTCGGTTTGGCGACTTGGTGTCCAGACTGTGGCTCGGATATCTTCACG
ACGCACGTGGAGGCGGAATTGGATGTCGTTCGGGCCATGCTATCCGACAC
GGAGCGGCGGAGAGAGGAACTGGGAGCCCGAATCGAAGCAAAAGATGTGG
AGAATTGCCTTGAAGACGTTGTTTCGATCTATGAGGCCGTATTGAAGGCG
CTGTTGGGCCGCTACTTGAAGCAAAAAGGGATGGAATCGGACGAAGTCGA
GAAATTGATGTCCAAGAAGGTCCGGAATGGCTTTCAGAATGTGGAACGAT
CTCGCGCGATTATATTGGAGCATACGGGACATGAATTGTTCGACACGATT
GACGGTTCAGCCGTAAGCGACCTGAAGAACGCGTTCGAAATGCGACATCC
AATTACACACAATCTTGGGGTGATTGATCGGAAGGTAACCTATAAGCCCA
AGCGAATCGCGGCACCGAGGCGACCTTATGAAGAGACTGTCCGACGGGAC
GTAATCTGCCCCAATTGCACCCTGGATCACGCGGTATTCGGTTTGGCGAC
TTGGTGTCCAGACTGTGGCTCGGATATCTTCACGACGCACGTGGAGGCGG
AATTGGATGTCGTTCGGGCCATGCTATCCGACACGGAGCGGCGGAGAGAG
GAACTGGGAGCCCGAATCGAAGCAAAAGATGTGGAGAATTGCCTTGAAGA
CGTTGTTTCGATCTATGAGGCCGTATTGAAGGCGCTGTTGGGCCGCTACT
TGAAGCAAAAAGGGATGGAATCGGACGAAGTCGAGAAATTGATGTCCAAG
AAGGTCCGGAATGGCTTTCAGAATGTGGAACGATCTCGCGCGATTATATT
GGAGCATACGGGACATGAATTGTTCGACACGATTGACGGTTCAGCCGTAA
GCGACCTGAAGAACGCGTTCGAAATGCGACATCCAATTACACACAATCTT
GGGGTGATTGATCGGAAG
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