mRNA_M-pyrifera_M_contig83822.19572.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig83822.19572.1
Unique NamemRNA_M-pyrifera_M_contig83822.19572.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Match: A0A813T5X9_9BILA (Beta-galactosidase n=1 Tax=Didymodactylos carnosus TaxID=1234261 RepID=A0A813T5X9_9BILA)

HSP 1 Score: 82.8 bits (203), Expect = 2.510e-15
Identity = 58/173 (33.53%), Postives = 84/173 (48.55%), Query Frame = 1
Query:   10 LGYDNGMTSGSIGKTKGIRESVKIGGVDLFHNTWTVRSDLAGNVYRVWDDEGGKNVTWSPN---PSTRALSWMQASF----PTPPLTSSQALVFNATGLGRGHFFVNGVDLGRYWTIP------------RAKSNPPTPTQQLYPIPTDVLHPTKSTQQNRIVVIEIIGATNP 471
            LG DNG+++ S  + KGI   V I GV + H  W  +  L G   +V+  +G   V W+        + ++W Q  F    P   L +S  L+ +A GLGRGH ++NG DLG YW I             +A+ N   PTQ+ Y IP D L      + N + + E +GA +P
Sbjct:  570 LGLDNGVSANSF-EYKGIVGDVTISGVSIKHAEWQHQKGLVGEYLQVYTAQGTSKVQWNHEWVESIQKPVTWFQTKFDLSVPIQDLNASP-LLMDANGLGRGHAYINGNDLGLYWLIAGECSDEPPCCCQQAQKNCMLPTQRFYHIPPDWLQ----AKNNLLTIFEDLGAQSP 736          
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Match: A0A816F452_9BILA (Hypothetical protein n=2 Tax=Adineta steineri TaxID=433720 RepID=A0A816F452_9BILA)

HSP 1 Score: 74.3 bits (181), Expect = 2.100e-12
Identity = 54/179 (30.17%), Postives = 86/179 (48.04%), Query Frame = 1
Query:    1 SENLGYDNGMTSGSIGKTKGIRESVKIGGVDLFHN---TWTVRSDLAGNVYRVWDDEGGKNVTWSPNPST---RALSWMQASFPTPPLT----SSQALVFNATGLGRGHFFVNGVDLGRYWTIP------------RAKSNPPTPTQQLYPIPTDVLHPTKSTQQNRIVVIEIIGATNP 471
            S +LG DN          KGI  +V + G  L  N    W  +  L G   +++ ++G K V W+P  +T   ++++W Q  F    L     ++  ++ +ATGL RGH FVNG D+G YW I             + +     P+Q+ Y IP+D L P    + N + + E +GA++P
Sbjct:  393 SVSLGIDNFNIGPGHFDYKGIVGNVSLDGQSLIGNETNVWEHQKGLFGEARQIYTEQGSKTVEWNPKWTTAVNKSVTWFQTRFDLNHLAREDLNANPVLLDATGLNRGHAFVNGNDIGLYWLIEGSCQISVPCCCLQREIRCGQPSQRYYHIPSDWLMP----KNNLLTIFEDLGASSP 567          
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Match: A0A8J6C2K9_DIALT (Glyco_hydro_35 domain-containing protein n=2 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J6C2K9_DIALT)

HSP 1 Score: 68.6 bits (166), Expect = 2.260e-10
Identity = 56/161 (34.78%), Postives = 76/161 (47.20%), Query Frame = 1
Query:    1 SENLGYDNGMTSGSIGKTKGIRESVKIGG-------VDLFHNTWTVRSDLAGNVYRVWDDEGGKNVTW---------SPNPSTRALSWMQASFPTPP--LTS--------SQALVFNATGLGRGHFFVNGVDLGRYWTIPRAKSNPPT---PTQQLYPIPT 396
            SE+LGY N      + K    R ++  GG        +L    WT+R+ L G   R+   +G   V W         SP    RAL+W +A F TP   LT+        +  L  N +G GRGH ++NG  +GRYW +PRA + P     PTQ LY +PT
Sbjct:  640 SEHLGYANYGFRAPLAKGLVGRVALLDGGGGGAPRASELTAGAWTIRAGLDGERLRLMGTQGQAAVRWGSARAGVDGSPAGRVRALTWYRAHFATPAELLTAPTERAGARAARLHVNMSGFGRGHAWLNGHAVGRYWLLPRA-TGPQAGVRPTQALYHLPT 799          
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Match: UPI0009017316 (beta-galactosidase 16-like n=2 Tax=Ipomoea TaxID=4119 RepID=UPI0009017316)

HSP 1 Score: 66.6 bits (161), Expect = 1.040e-9
Identity = 43/140 (30.71%), Postives = 69/140 (49.29%), Query Frame = 1
Query:   91 DLFHNTWTVRSDLAGNVYRVWDDEGGKNVTWSP-NPSTRALSWMQASFPTPPLTSSQALVFNATGLGRGHFFVNGVDLGRYWTIPRAKSNPPTPTQQLYPIPTDVLHPTKSTQQNRIVVIEIIGATNPTLPAIQTISLQK 507
            DL H+ W  +  L G   ++++++    V WS  + S + L W + +F  P    +  +  N   +G+GH +VNG  +GRYW   R  S   TP+Q  Y IP   +  +K    N +V+ E  G  NP    I ++S+ K
Sbjct:  567 DLRHSEWGYQVGLLGEKLQIFEEDASNKVQWSKFSYSNQPLIWYKTTFDAP--RGNGPIALNLGSMGKGHVWVNGQSIGRYWVSFRTPSG--TPSQAWYNIPRSFVRASK----NSVVLFEEEGG-NPLGITIDSVSISK 697          
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Match: A0A151SBP2_CAJCA (Beta-galactosidase n=2 Tax=Cajanus cajan TaxID=3821 RepID=A0A151SBP2_CAJCA)

HSP 1 Score: 63.9 bits (154), Expect = 8.940e-9
Identity = 47/160 (29.38%), Postives = 74/160 (46.25%), Query Frame = 1
Query:   88 VDLFHNTWTVRSDLAGNVYRVWDDEGGKNVTWSPN---PSTRALSWMQASFPTPPLTSSQALVFNATGLGRGHFFVNGVDLGRYW--------------------TIPRAKSNPPTPTQQLYPIPTDVLHPTKSTQQNRIVVIEIIGATNPTLPAIQTIS 498
            +DL  N W+ +  L G   R++D +    V+W  N   P +R ++W +A F  P       +V +  GLG+GH +VNG  +GRYW                    T     +N   P+Q+ Y +P   L+  K+T    +V+ E IG  NP   + QT++
Sbjct:  573 MDLSTNLWSYKIGLNGERKRLYDLQPHIGVSWHANSSYPISRPMTWYKADFEAP--YGKNPVVVDLQGLGKGHAWVNGHSIGRYWPSWITATNGCNDTCDYHGKYTAENCNTNCGNPSQRWYHVPRSFLNNDKNT----LVLFEEIGG-NPQNVSFQTVT 725          
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Match: A0A7R9ZKE6_9STRA (Hypothetical protein n=1 Tax=Craspedostauros australis TaxID=1486917 RepID=A0A7R9ZKE6_9STRA)

HSP 1 Score: 63.2 bits (152), Expect = 1.550e-8
Identity = 56/186 (30.11%), Postives = 78/186 (41.94%), Query Frame = 1
Query:    1 SENLGYDNGMTSGSIG---KTKGIRESVKIGGVDLFHNTWTVRSDLAGNVYRVW---------------------DDEGGK----NVTWSPNPSTRALSWMQASFPTPPLTSSQALVFNATGLGRGHFFVNGVDLGRYWTIPRAKSNPPTPTQQLYPIPTDVLHPTKSTQQNRIVVIEIIGATNPT 474
            SE+LGYDN +    +    K KGI  SV +GG     +T  V         R W                     DD  G     +V      S+    W Q +F TPP  S    +F     GRGH ++NG DLGR+W I +A S+   PTQ  Y +P D L+       N + + ++ G+ + T
Sbjct:  370 SESLGYDNLIGRWGVDTKRKMKGIVGSVVLGGSQDGSSTSIVVQSETMASGRRWLSFPGLHWDLGNIRNNNPNDGDDHDGSIEKTDVAAGIATSSVQARWAQTTFQTPPFDSPHRSLFLKVSEGRGHIWLNGHDLGRFWNITKAGSSN-RPTQLYYQLPPDYLNKADG-DANDLTIFDVFGSAHVT 553          
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Match: UPI0018FFB470 (beta-galactosidase 7-like n=1 Tax=Benincasa hispida TaxID=102211 RepID=UPI0018FFB470)

HSP 1 Score: 63.2 bits (152), Expect = 1.650e-8
Identity = 44/161 (27.33%), Postives = 75/161 (46.58%), Query Frame = 1
Query:   91 DLFHNTWTVRSDLAGNVYRVWDDEGGKNVTWSPNPST---RALSWMQASFPTPPLTSSQALVFNATGLGRGHFFVNGVDLGRYWTI--------------------PRAKSNPPTPTQQLYPIPTDVLHPTKSTQQNRIVVIEIIGATNPTLPAIQTISLQ 504
            DL +NTW+ +  L G + + ++    + + W         R ++W + SF TP  + +  +V +  G+G+GH +VNG+ +GR+W                       +   N   PTQ+ Y +P   L    S   N +++ E IG  NP   +IQTI+L+
Sbjct:  577 DLSYNTWSYKVGLNGEMEQFYNPLLTQKIKWDALNKISIGRRMTWYKTSFKTP--SGTDPVVLDMQGMGKGHAWVNGLSIGRFWPSLIVGNDGCNSTCDYRGAYDSKKCAGNCGNPTQRWYHVPRSFL----SNDTNTLILFEEIGG-NPQQVSIQTIALR 730          
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Match: A0A427AIB2_ENSVE (Beta-galactosidase n=1 Tax=Ensete ventricosum TaxID=4639 RepID=A0A427AIB2_ENSVE)

HSP 1 Score: 62.4 bits (150), Expect = 2.990e-8
Identity = 43/147 (29.25%), Postives = 64/147 (43.54%), Query Frame = 1
Query:   82 GGVDLFHNTWTVRSDLAGNVYRVWDDEGGKNVTWSPNP---STRALSWMQASFPTPPLTSSQALVFNATGLGRGHFFVNGVDLGRYWTI------------------PRAKSNPPTPTQQLYPIPTDVLHPTKSTQQNRIVVIEIIG 459
            G  DL   TW+ +  L G    +   EG  +V W       S + L+W +A F  P     + L  +   +G+GH ++NG  +GRYWT                   P+ +S    PTQ+ Y +P   L PT+    N +VV E +G
Sbjct:  446 GSRDLTWQTWSYQVGLKGEDMNLNSLEGASSVEWMQGSLVQSQQPLTWYRAYFDAP--DGDEPLALDMASMGKGHVWINGQSIGRYWTAYAPEENCKSCSYTGTYRAPKCQSGCGQPTQRWYHVPRSWLQPTR----NLLVVFEELG 586          
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Match: A0A151UGP6_CAJCA (Beta-galactosidase 7 n=3 Tax=Cajanus cajan TaxID=3821 RepID=A0A151UGP6_CAJCA)

HSP 1 Score: 61.2 bits (147), Expect = 3.400e-8
Identity = 45/160 (28.12%), Postives = 73/160 (45.62%), Query Frame = 1
Query:   88 VDLFHNTWTVRSDLAGNVYRVWDDEGGKNVTWSPN---PSTRALSWMQASFPTPPLTSSQALVFNATGLGRGHFFVNGVDLGRYW--------------------TIPRAKSNPPTPTQQLYPIPTDVLHPTKSTQQNRIVVIEIIGATNPTLPAIQTIS 498
            +DL  N W+ +  L G   R++D +    V W  N   P  + ++W +A F  P    +  +V +  GLG+GH +VNG  +GRYW                       +  +N   P+Q+ Y +P   L+  K+T    +V+ E IG  NP   + QT++
Sbjct:    1 MDLSTNLWSYKIGLNGERKRLYDLQPHTGVAWHANSSYPIGKPMTWYKADFEAP--YGTNPVVVDLQGLGKGHAWVNGHSIGRYWPSWITATNGCTDTCDYRGNYVTEKCNTNCGNPSQRWYHVPRSFLNNDKNT----LVLFEEIGG-NPQNVSFQTVT 153          
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Match: A0A5B6ZWM8_DAVIN (Beta-galactosidase n=1 Tax=Davidia involucrata TaxID=16924 RepID=A0A5B6ZWM8_DAVIN)

HSP 1 Score: 62.0 bits (149), Expect = 4.150e-8
Identity = 44/160 (27.50%), Postives = 74/160 (46.25%), Query Frame = 1
Query:   88 VDLFHNTWTVRSDLAGNVYRVWDDEGGKNVTWSPN--PSTRALSWMQASFPTPPLTSSQALVFNATGLGRGHFFVNGVDLGRYW--------------------TIPRAKSNPPTPTQQLYPIPTDVLHPTKSTQQNRIVVIEIIGATNPTLPAIQTISL 501
            +DL  + W+ +  L G   ++++D   K   WS +  P  R ++W + SF  P    +  +V +  G+G+GH +VNG  LGR+W                    +  +   N   PTQ+ Y +P   L   K+T    +V+ E  G  NPT  + QT+++
Sbjct:  583 MDLSSHQWSYKVGLNGEAKQLYNDLPAKKAKWSSSNLPILRPMTWYKTSFIAP--LGTDPVVVDLQGMGKGHAWVNGHSLGRFWPTFLASDDGCSDTCDYRGLYSSGKCVKNCGNPTQRWYHVPRSFLRGDKNT----LVLFEEFGG-NPTQVSFQTVTI 735          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig83822.19572.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A813T5X9_9BILA2.510e-1533.53Beta-galactosidase n=1 Tax=Didymodactylos carnosus... [more]
A0A816F452_9BILA2.100e-1230.17Hypothetical protein n=2 Tax=Adineta steineri TaxI... [more]
A0A8J6C2K9_DIALT2.260e-1034.78Glyco_hydro_35 domain-containing protein n=2 Tax=D... [more]
UPI00090173161.040e-930.71beta-galactosidase 16-like n=2 Tax=Ipomoea TaxID=4... [more]
A0A151SBP2_CAJCA8.940e-929.38Beta-galactosidase n=2 Tax=Cajanus cajan TaxID=382... [more]
A0A7R9ZKE6_9STRA1.550e-830.11Hypothetical protein n=1 Tax=Craspedostauros austr... [more]
UPI0018FFB4701.650e-827.33beta-galactosidase 7-like n=1 Tax=Benincasa hispid... [more]
A0A427AIB2_ENSVE2.990e-829.25Beta-galactosidase n=1 Tax=Ensete ventricosum TaxI... [more]
A0A151UGP6_CAJCA3.400e-828.13Beta-galactosidase 7 n=3 Tax=Cajanus cajan TaxID=3... [more]
A0A5B6ZWM8_DAVIN4.150e-827.50Beta-galactosidase n=1 Tax=Davidia involucrata Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig83822contigM-pyrifera_M_contig83822:1..507 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score79.7
Seed ortholog evalue1.3e-12
Seed eggNOG ortholog38727.Pavir.J00811.1.p
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0003006,GO:0003674,GO:0003824,GO:0004553,GO:0004565,GO:0005575,GO:0005576,GO:0005618,GO:0005622,GO:0005623,GO:0005737,GO:0005773,GO:0007275,GO:0008150,GO:0008152,GO:0009058,GO:0009664,GO:0009791,GO:0009827,GO:0009987,GO:0010154,GO:0010191,GO:0010192,GO:0010214,GO:0015925,GO:0016043,GO:0016787,GO:0016798,GO:0022414,GO:0030312,GO:0032501,GO:0032502,GO:0042545,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0044237,GO:0044249,GO:0044424,GO:0044444,GO:0044464,GO:0045229,GO:0048046,GO:0048316,GO:0048354,GO:0048359,GO:0048608,GO:0048731,GO:0048856,GO:0061458,GO:0071554,GO:0071555,GO:0071669,GO:0071840,GO:0071944
EggNOG free text desc.beta-galactosidase
EggNOG OGs37R16@33090,3GCHZ@35493,3IEAE@38820,3KMCB@4447,COG1874@1,KOG0496@2759
COG Functional cat.G
Best tax levelPoales
Best eggNOG OGNA|NA|NA
Exons1
Model size507
Cds size480
Stop0
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816746.13508-UTR-M-pyrifera_M_contig83822:0..271622816746.13508-UTR-M-pyrifera_M_contig83822:0..27Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig83822 1..27 +
1692278153.505234-UTR-M-pyrifera_M_contig83822:0..271692278153.505234-UTR-M-pyrifera_M_contig83822:0..27Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig83822 1..27 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816746.1455243-CDS-M-pyrifera_M_contig83822:27..5071622816746.1455243-CDS-M-pyrifera_M_contig83822:27..507Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83822 28..507 +
1692278153.5207274-CDS-M-pyrifera_M_contig83822:27..5071692278153.5207274-CDS-M-pyrifera_M_contig83822:27..507Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83822 28..507 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig83822.19572.1prot_M-pyrifera_M_contig83822.19572.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig83822 28..507 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig83822.19572.1

>prot_M-pyrifera_M_contig83822.19572.1 ID=prot_M-pyrifera_M_contig83822.19572.1|Name=mRNA_M-pyrifera_M_contig83822.19572.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=160bp
MTSGSIGKTKGIRESVKIGGVDLFHNTWTVRSDLAGNVYRVWDDEGGKNV
TWSPNPSTRALSWMQASFPTPPLTSSQALVFNATGLGRGHFFVNGVDLGR
YWTIPRAKSNPPTPTQQLYPIPTDVLHPTKSTQQNRIVVIEIIGATNPTL
PAIQTISLQK
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mRNA from alignment at M-pyrifera_M_contig83822:1..507+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig83822.19572.1 ID=mRNA_M-pyrifera_M_contig83822.19572.1|Name=mRNA_M-pyrifera_M_contig83822.19572.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=507bp|location=Sequence derived from alignment at M-pyrifera_M_contig83822:1..507+ (Macrocystis pyrifera P11B4 male)
TCTGAGAATCTGGGATACGACAACGGAATGACTTCAGGGAGTATAGGGAA GACAAAAGGCATCCGGGAGAGTGTGAAAATCGGAGGGGTGGATTTGTTCC ACAACACTTGGACAGTTCGCTCAGACCTCGCAGGCAATGTGTACAGAGTG TGGGATGACGAGGGGGGCAAGAATGTCACGTGGAGTCCCAACCCATCCAC CAGAGCTCTGTCTTGGATGCAAGCCTCCTTCCCCACCCCTCCTCTCACCT CCTCCCAAGCCCTCGTCTTCAACGCGACAGGGTTGGGGAGAGGGCATTTT TTCGTCAACGGGGTTGATCTGGGAAGGTACTGGACAATTCCCCGGGCCAA ATCCAACCCCCCAACGCCCACGCAACAACTCTACCCCATCCCAACCGACG TCCTCCACCCAACAAAGAGCACCCAGCAAAACAGAATCGTGGTCATCGAA ATCATAGGCGCCACAAACCCAACCCTTCCCGCCATCCAAACCATCTCACT CCAGAAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig83822:1..507+

>mRNA_M-pyrifera_M_contig83822.19572.1 ID=mRNA_M-pyrifera_M_contig83822.19572.1|Name=mRNA_M-pyrifera_M_contig83822.19572.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=960bp|location=Sequence derived from alignment at M-pyrifera_M_contig83822:1..507+ (Macrocystis pyrifera P11B4 male)
ATGACTTCAGGGAGTATAGGGAAGACAAAAGGCATCCGGGAGAGTGTGAA
AATCGGAGGGGTGGATTTGTTCCACAACACTTGGACAGTTCGCTCAGACC
TCGCAGGCAATGTGTACAGAGTGTGGGATGACGAGGGGGGCAAGAATGTC
ACGTGGAGTCCCAACCCATCCACCAGAGCTCTGTCTTGGATGCAAGCCTC
CTTCCCCACCCCTCCTCTCACCTCCTCCCAAGCCCTCGTCTTCAACGCGA
CAGGGTTGGGGAGAGGGCATTTTTTCGTCAACGGGGTTGATCTGGGAAGG
TACTGGACAATTCCCCGGGCCAAATCCAACCCCCCAACGCCCACGCAACA
ACTCTACCCCATCCCAACCGACGTCCTCCACCCAACAAAGAGCACCCAGC
AAAACAGAATCGTGGTCATCGAAATCATAGGCGCCACAAACCCAACCCTT
CCCGCCATCCAAACCATCTCACTCCAGAAAATGACTTCAGGGAGTATAGG
GAAGACAAAAGGCATCCGGGAGAGTGTGAAAATCGGAGGGGTGGATTTGT
TCCACAACACTTGGACAGTTCGCTCAGACCTCGCAGGCAATGTGTACAGA
GTGTGGGATGACGAGGGGGGCAAGAATGTCACGTGGAGTCCCAACCCATC
CACCAGAGCTCTGTCTTGGATGCAAGCCTCCTTCCCCACCCCTCCTCTCA
CCTCCTCCCAAGCCCTCGTCTTCAACGCGACAGGGTTGGGGAGAGGGCAT
TTTTTCGTCAACGGGGTTGATCTGGGAAGGTACTGGACAATTCCCCGGGC
CAAATCCAACCCCCCAACGCCCACGCAACAACTCTACCCCATCCCAACCG
ACGTCCTCCACCCAACAAAGAGCACCCAGCAAAACAGAATCGTGGTCATC
GAAATCATAGGCGCCACAAACCCAACCCTTCCCGCCATCCAAACCATCTC
ACTCCAGAAA
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