mRNA_M-pyrifera_M_contig8379.19561.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig8379.19561.1
Unique NamemRNA_M-pyrifera_M_contig8379.19561.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Match: D7FMZ9_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FMZ9_ECTSI)

HSP 1 Score: 248 bits (633), Expect = 4.580e-81
Identity = 124/163 (76.07%), Postives = 135/163 (82.82%), Query Frame = 1
Query:   82 MKQSKFTKRIFRIFDEDGSGSIDFREFVMSLWNYCTLSKAALIMFAFDLXXXXXXXXXXIGEVELLLKEVYGREFQTSSQAQAIMAKLKSGENDMKRDINVDSFREFCRTHPAVLYPAFVLQTQMQNKILGSKFWASCGKSRVQLSDGKYVDITDLLQAHVNQ 570
            MKQSKFTKRIFRIFDEDGSGS+DFREFVMSLWNYCTLSKAALIMFAFDL          IGEVELLLKEVYGREFQ+++ AQ IMAKL+  E  + R+INVD FREFCRTHPAVLYPAFVLQTQMQ KILGSKFW +C  +RV LS G YVDI  +L AHVN+
Sbjct:    1 MKQSKFTKRIFRIFDEDGSGSVDFREFVMSLWNYCTLSKAALIMFAFDLYDNDNSGEIDIGEVELLLKEVYGREFQSNTHAQTIMAKLRGSELGLARNINVDQFREFCRTHPAVLYPAFVLQTQMQEKILGSKFWIACSNARVALSSGAYVDIAAILHAHVNE 163          
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Match: A0A7S3XL51_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3XL51_HETAK)

HSP 1 Score: 165 bits (417), Expect = 7.910e-48
Identity = 94/188 (50.00%), Postives = 128/188 (68.09%), Query Frame = 1
Query:   25 IDEDGSGEISLWELFGALEMKQSKFTKRIFRIFDEDGSGSIDFREFVMSLWNYCTLSKAALIMFAFDLXXXXXXXXXXIGEVELLLKEVYGREFQTSSQAQAIMAKLKSGEND------MKR-DINVDSFREFCRTHPAVLYPAFVLQTQMQNKILGSKFWASCGKSRVQLSDGKYVDITDLLQAHVN 567
            ID D SG I + EL   L +  S+FT R+FRIFDEDGSG +DFREFV+S+WNYCTL+K AL+MFAFDLXXXXXXXXXX  EVEL+LKEVYG  FQ S  AQ ++  ++  + D      M+R ++  + F +FCR H  +L PAF +Q  ++  + G + W    + R ++SDG Y+ +  LL++H+N
Sbjct:   47 IDGDNSGTIEVKELMAYLGL-HSQFTNRVFRIFDEDGSGELDFREFVVSMWNYCTLAKNALVMFAFDLXXXXXXXXXXXXEVELMLKEVYGEGFQASQLAQKVLRHMRMIDGDARLPLSMQRCEVTAEQFGQFCRKHATLLLPAFTMQETLREAVGGKRLWERLSRRRFEVSDGAYISVNQLLESHLN 233          
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Match: A0A2R5GVS2_9STRA (Calcium-dependent protein kinase 1 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5GVS2_9STRA)

HSP 1 Score: 165 bits (417), Expect = 4.490e-47
Identity = 86/186 (46.24%), Postives = 119/186 (63.98%), Query Frame = 1
Query:   22 QIDEDGSGEISLWELFGALEMKQSKFTKRIFRIFDEDGSGSIDFREFVMSLWNYCTLSKAALIMFAFDLXXXXXXXXXXIGEVELLLKEVYGREFQTSSQAQAIMAKLKSGENDMKRD---INVDSFREFCRTHPAVLYPAFVLQTQMQNKILGSKFWASCGKSRVQLSDGKYVDITDLLQAHVNQ 570
            +ID+DGSGE+SL+EL   L++ ++ FTKR+F IFDEDGSGSIDFREFV+S WNY TL+K+ LI+FAFDL          + E+  + K+VYG +F+ S  A  I+ K+    ND  R    I ++ F  FC  HP +LYPAF  Q  +Q  I+G  FW    K R+ + +G  V I D+L AH+++
Sbjct:   40 KIDKDGSGELSLFELLDHLKVSKTPFTKRVFSIFDEDGSGSIDFREFVLSTWNYATLTKSTLIIFAFDLYDGDESGSMELEEIMDMCKDVYGADFEKSRLAAQIIDKIHKL-NDPTRGSGIITLEKFAVFCEHHPGLLYPAFTFQQALQKAIIGVTFWTKLSKERLVMPNGVQVSIKDVLTAHIDK 224          
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Match: A0A7S2F8V1_9STRA (Hypothetical protein (Fragment) n=1 Tax=Florenciella parvula TaxID=236787 RepID=A0A7S2F8V1_9STRA)

HSP 1 Score: 158 bits (400), Expect = 2.070e-46
Identity = 88/149 (59.06%), Postives = 114/149 (76.51%), Query Frame = 1
Query:   22 QIDEDGSGEISLWELFGALEMKQSKFTKRIFRIFDEDGSGSIDFREFVMSLWNYCTLSKAALIMFAFDLXXXXXXXXXXIGEVELLLKEVYGREFQTSSQAQAIMAKL-KSGENDMKRDINVDSFREFCRTHPAVLYPAFVLQTQMQNK 465
            +ID+DGSG ISLWE+   L+++++KFTKR+F I DED SG IDFREFV++LWNYCTL KAALI+FAFD XXXXXXXXXX  E++L+LKEVYG++   S QAQ +M K+ K  E+    +I  + F +F R +PA+LYPAF LQ  +Q K
Sbjct:    1 KIDQDGSGSISLWEMLEHLQLQRNKFTKRVFSIMDEDNSGEIDFREFVIALWNYCTLGKAALILFAFDXXXXXXXXXXXXDEIKLMLKEVYGKDVSNSQQAQMVMGKIQKFSESKADEEIGKEEFSDFVRRNPALLYPAFQLQMTLQRK 149          
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Match: A0A482T6P1_9ARCH (Uncharacterized protein n=1 Tax=archaeon TaxID=1906665 RepID=A0A482T6P1_9ARCH)

HSP 1 Score: 155 bits (393), Expect = 2.070e-44
Identity = 74/176 (42.05%), Postives = 111/176 (63.07%), Query Frame = 1
Query:   25 IDEDGSGEISLWELFGALEMKQSKFTKRIFRIFDEDGSGSIDFREFVMSLWNYCTLSKAALIMFAFDLXXXXXXXXXXIGEVELLLKEVYGREFQTSSQAQAIMAKLKSGENDMKRDINVDSFREFCRTHPAVLYPAFVLQTQMQNKILGSKFWASCGKSRVQLSDGKYVDITDLL 552
            ID D SG I L EL   L+++ + F+ R F +FD + SG IDFREFV+SLWNYCTL  A L ++ FDL            EV+ +LK++YG+ FQT+ +A A+M  +K  E  ++ D ++  F +F R+H  +L+PAF +Q ++Q K+ G  FW  C   R+++S GKY  IT+++
Sbjct:   40 IDRDNSGAIDLQELLNYLDIENTSFSHRAFSVFDINNSGKIDFREFVLSLWNYCTLGNATLDVYTFDLYDKDKSGILSESEVQAMLKDLYGKTFQTNPRATAVMNAMKKSE--LRGDFDLPKFTKFVRSHQEMLFPAFQMQLKIQEKVFGRGFWERCSNRRIEISKGKYASITEIM 213          
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Match: A0A4Q3A667_9BACT (Uncharacterized protein (Fragment) n=1 Tax=Verrucomicrobiaceae bacterium TaxID=2026800 RepID=A0A4Q3A667_9BACT)

HSP 1 Score: 155 bits (392), Expect = 4.090e-43
Identity = 83/183 (45.36%), Postives = 119/183 (65.03%), Query Frame = 1
Query:   22 QIDEDGSGEISLWELFGALEMKQSKFTKRIFRIFDEDGSGSIDFREFVMSLWNYCTLSKAALIMFAFDLXXXXXXXXXXIGEVELLLKEVYGREF-QTSSQAQAIMAKLKSGENDMKRDINVDSFREFCRTHPAVLYPAFVLQTQMQNKILGSKFWASCGKSRVQLSDGKYVDITDLLQAHVN 567
            ++D D SG + L EL   LEM ++ F  R+F IFDEDGSG IDFREFV+SLWNYCTLSKA L +FAFDL            E E LL+++YG  + Q +  A+ +  +LK    +   DI  D F+ F + HP++L+PAF LQ  +++++LGS FW    + R++LSDGK+V I+ L++ +V+
Sbjct:   39 RVDVDHSGTVGLKELLNYLEMGEAVFKDRVFSIFDEDGSGQIDFREFVLSLWNYCTLSKATLDLFAFDLYDKNNDGELSAEEAEDLLRDLYGAHYAQHNPTARFVAFELKKVCAESSLDI--DRFQNFIQYHPSLLFPAFQLQHALRSRVLGSGFWERASERRIELSDGKFVPISRLMELNVS 219          
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Match: A0A7S3HMR9_9STRA (Hypothetical protein n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3HMR9_9STRA)

HSP 1 Score: 151 bits (381), Expect = 4.250e-39
Identity = 84/182 (46.15%), Postives = 128/182 (70.33%), Query Frame = 1
Query:   22 QIDEDGSGEISLWELFGALEMKQSKFTKRIFRIFDEDGSGSIDFREFVMSLWNYCTLSKAALIMFAFDLXXXXXXXXXXIGEVELLLKEVYGREFQTSSQAQAIMAKLKSGENDMKRDINVDSFREFCRTHPAVLYPAFVLQTQMQNKILGSKFWASCGKSRVQLSDGKYVDITDLLQAHVN 567
            ++D D SG+I+L EL   +++++++FT+RIF IFD DGSG IDFREFV+SLWNYCTL+KA L MFAF  XXXXXXXXXX  EV  +L+++YG++   S+     +A      +D    +N++ +R+F RTH A+L+PAF +Q  +Q K+LG +FW    + R++ S+GKY+ + + +  +V+
Sbjct:   42 RVDFDKSGQIALAELLAHIDLERTRFTERIFSIFDADGSGEIDFREFVLSLWNYCTLTKATLDMFAFXXXXXXXXXXXXGKEVTQMLQDIYGKKEVKSNYLARTVANDLKAIDDSGTLLNIEEYRQFVRTHQALLFPAFQMQLALQRKVLGVRFWERNAERRLKFSNGKYISVGNFILMNVD 223          
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Match: A0A7S1CAR3_9STRA (Hypothetical protein n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CAR3_9STRA)

HSP 1 Score: 142 bits (358), Expect = 2.720e-37
Identity = 86/189 (45.50%), Postives = 125/189 (66.14%), Query Frame = 1
Query:   28 DEDGSGEISLWELFGALEMKQSKFTKRIFRIFDEDGSGSIDFREFVMSLWNYCTLSKAALIMFAFDLXXXXXXXXXXIGEVELLLKEVYGREFQTSSQAQAIMAKLK----SGENDMKRDINVDS--FREFCRTHPAVLYPAFVLQTQMQNKILGSKFWASCGKSRVQLSDGKYVDITDLLQ--AHVNQ 570
            DED SGEIS+ E F  ++++++KF KR F +FD DGSG IDFREFV+S+WNYCT ++ +LI F    XXXXXXXXXX   ++L++KEVYG  F+TS+ A+ IM K+     +   D+K    +D   F  F + HPA+L+PAF LQ ++Q +++G  FW    K R +L    + D T+L +  +H+N+
Sbjct:   47 DEDNSGEISVKEFFDYVDLERTKFAKRAFSLFDHDGSGQIDFREFVVSMWNYCTFTQQSLIEFXXXXXXXXXXXXXXXXXMQLIVKEVYGSSFRTSAYAKKIMEKVAQIGHASSLDVKTAAQIDKGMFARFTQKHPAMLFPAFRLQQELQKRVMGVAFWKRAAKKRARL----FADSTNLKEFMSHINE 231          
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Match: A0A7S4A064_9STRA (Hypothetical protein n=1 Tax=Pelagomonas calceolata TaxID=35677 RepID=A0A7S4A064_9STRA)

HSP 1 Score: 144 bits (364), Expect = 4.560e-37
Identity = 84/186 (45.16%), Postives = 125/186 (67.20%), Query Frame = 1
Query:   19 VQIDEDGSGEISLWELFGALEMKQSKFTKRIFRIFDEDGSGSIDFREFVMSLWNYCTLSKAALIMFAFDLXXXXXXXXXXIGEVELLLKEVYGREFQTSSQAQAIMAKLKSGENDMKRD-INVDSFREFCRTHPAVLYPAFVLQTQMQNKILGSKFWASCGKSRVQLSDGKY-VDITDLLQAHVNQ 570
            V ID+DGSG I LWEL   L++++++F KR+F IFD DGS  IDF+EFV++LW YCTL +  L+MF    XXXXXXXXXX      +LKE+YG+ +  ++ A  ++  + +       D ++V++F EFCRTHPA+L PAF +QT M+ +I+G  +W      RV++  G++ + I DLL+AH+ +
Sbjct:  268 VIIDKDGSGAIELWELLDYLDLQRNRFAKRVFAIFDADGSNQIDFKEFVVALWQYCTLGRPQLVMFXXXXXXXXXXXXXXXXXFNAMLKELYGKRYSKNTTAVNLLRHINAMTKRRDTDEVDVEAFAEFCRTHPAMLMPAFAMQTTMRARIMGGAWWDRRSNERVKV--GRHTMRIHDLLKAHLQE 451          
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Match: A0A7S3M126_9STRA (Hypothetical protein n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3M126_9STRA)

HSP 1 Score: 144 bits (362), Expect = 5.290e-37
Identity = 69/181 (38.12%), Postives = 111/181 (61.33%), Query Frame = 1
Query:   22 QIDEDGSGEISLWELFGALEMKQSKFTKRIFRIFDEDGSGSIDFREFVMSLWNYCTLSKAALIMFAFDLXXXXXXXXXXIGEVELLLKEVYGR-EFQTSSQAQAIMAKLKSGENDMKRDINVDSFREFCRTHPAVLYPAFVLQTQMQNKILGSKFWASCGKSRVQLSDGKYVDITDLLQAH 561
            ++D D SG I L EL   L++  + +T+++F IFDED SG ++F+EFVM+LWNYCTL+ + L MFAFDL            EV  +L +++G+ E +T+  A+ + A+L     + +    +D+FR F +TH A+L+PAF +Q  +Q K LG+ FW      R+++ +GKY+ +   +  H
Sbjct:   43 KVDMDNSGSIGLPELLAHLDLPVTDYTQKVFSIFDEDRSGEVEFKEFVMTLWNYCTLTSSTLEMFAFDLYDSDGSGEISPNEVTQMLTDLFGKHEVKTNQHAKVMAAELLDHAKNFRDPFTIDTFRAFVKTHQALLFPAFQMQLALQRKFLGTAFWEKNADRRMEICNGKYISVGAFIAEH 223          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig8379.19561.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
D7FMZ9_ECTSI4.580e-8176.07Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A7S3XL51_HETAK7.910e-4850.00Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A2R5GVS2_9STRA4.490e-4746.24Calcium-dependent protein kinase 1 n=1 Tax=Hondaea... [more]
A0A7S2F8V1_9STRA2.070e-4659.06Hypothetical protein (Fragment) n=1 Tax=Florenciel... [more]
A0A482T6P1_9ARCH2.070e-4442.05Uncharacterized protein n=1 Tax=archaeon TaxID=190... [more]
A0A4Q3A667_9BACT4.090e-4345.36Uncharacterized protein (Fragment) n=1 Tax=Verruco... [more]
A0A7S3HMR9_9STRA4.250e-3946.15Hypothetical protein n=1 Tax=Spumella elongata Tax... [more]
A0A7S1CAR3_9STRA2.720e-3745.50Hypothetical protein n=1 Tax=Bicosoecida sp. CB-20... [more]
A0A7S4A064_9STRA4.560e-3745.16Hypothetical protein n=1 Tax=Pelagomonas calceolat... [more]
A0A7S3M126_9STRA5.290e-3738.12Hypothetical protein n=1 Tax=Spumella elongata Tax... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig8379contigM-pyrifera_M_contig8379:2118..2936 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score250.8
Seed ortholog evalue5.3e-64
Seed eggNOG ortholog2880.D7FMZ9
Hectar predicted targeting categoryother localisation
EggNOG free text desc.EF-hand, calcium binding motif
EggNOG OGs2CXXS@1,2S4MR@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons2
Model size570
Cds size570
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816745.6704893-CDS-M-pyrifera_M_contig8379:2117..22331622816745.6704893-CDS-M-pyrifera_M_contig8379:2117..2233Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8379 2118..2233 +
1692278153.251181-CDS-M-pyrifera_M_contig8379:2117..22331692278153.251181-CDS-M-pyrifera_M_contig8379:2117..2233Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8379 2118..2233 +
1622816745.6827624-CDS-M-pyrifera_M_contig8379:2482..29361622816745.6827624-CDS-M-pyrifera_M_contig8379:2482..2936Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8379 2483..2936 +
1692278153.2700546-CDS-M-pyrifera_M_contig8379:2482..29361692278153.2700546-CDS-M-pyrifera_M_contig8379:2482..2936Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig8379 2483..2936 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig8379.19561.1prot_M-pyrifera_M_contig8379.19561.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig8379 2118..2936 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig8379.19561.1

>prot_M-pyrifera_M_contig8379.19561.1 ID=prot_M-pyrifera_M_contig8379.19561.1|Name=mRNA_M-pyrifera_M_contig8379.19561.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=190bp
MPCDAPVQIDEDGSGEISLWELFGALEMKQSKFTKRIFRIFDEDGSGSID
FREFVMSLWNYCTLSKAALIMFAFDLYDNDNSGEIDIGEVELLLKEVYGR
EFQTSSQAQAIMAKLKSGENDMKRDINVDSFREFCRTHPAVLYPAFVLQT
QMQNKILGSKFWASCGKSRVQLSDGKYVDITDLLQAHVNQ
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mRNA from alignment at M-pyrifera_M_contig8379:2118..2936+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig8379.19561.1 ID=mRNA_M-pyrifera_M_contig8379.19561.1|Name=mRNA_M-pyrifera_M_contig8379.19561.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=819bp|location=Sequence derived from alignment at M-pyrifera_M_contig8379:2118..2936+ (Macrocystis pyrifera P11B4 male)
ATGCCATGTGATGCCCCTGTGCAGATCGACGAGGACGGATCAGGGGAAAT CAGTCTGTGGGAGTTGTTCGGAGCTCTGGAAATGAAGCAGAGCAAATTTA CCAAGCGAATTTTCAGGTACCAAGAGTGAGAAGGACGTGAATGCCTACTA CTGTAGTGCGGGTATTATACTCGAACAAGTCGCGGGAAACAGGGATGTCC ATGCGAGAGGCGTCTTACTGCATTGCGGCTATAAGGCAGTCGCACGCACA ACTCTTAGTGCTGACTGACCCATAGGGTGTATCCGCTTCTTAGCATGAGA AAAGTCGCGTTCACTAATGAATCCACGTTTAATTCCTCTCGTTCTGAACG ATGCCACCACGGTAGGATTTTCGACGAAGATGGCAGCGGGTCCATCGACT TCCGAGAGTTCGTGATGAGTCTGTGGAACTACTGCACCCTCTCCAAAGCG GCGTTGATAATGTTTGCGTTCGACCTGTACGACAACGACAACAGCGGCGA GATCGACATTGGCGAGGTCGAACTGCTGCTGAAGGAGGTGTACGGGAGAG AGTTTCAGACTAGCTCCCAGGCGCAGGCAATTATGGCGAAGCTCAAGAGC GGAGAAAATGACATGAAGAGAGATATCAACGTAGACAGCTTCCGAGAGTT TTGTCGGACCCACCCGGCCGTTTTGTACCCAGCGTTCGTGTTGCAGACGC AGATGCAGAACAAGATACTGGGCTCTAAGTTTTGGGCTTCGTGCGGCAAA AGCCGTGTGCAATTGTCCGACGGGAAGTACGTCGACATCACAGATCTTCT GCAAGCTCACGTCAACCAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig8379:2118..2936+

>mRNA_M-pyrifera_M_contig8379.19561.1 ID=mRNA_M-pyrifera_M_contig8379.19561.1|Name=mRNA_M-pyrifera_M_contig8379.19561.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1140bp|location=Sequence derived from alignment at M-pyrifera_M_contig8379:2118..2936+ (Macrocystis pyrifera P11B4 male)
ATGCCATGTGATGCCCCTGTGCAGATCGACGAGGACGGATCAGGGGAAAT
CAGTCTGTGGGAGTTGTTCGGAGCTCTGGAAATGAAGCAGAGCAAATTTA
CCAAGCGAATTTTCAGATGCCATGTGATGCCCCTGTGCAGATCGACGAGG
ACGGATCAGGGGAAATCAGTCTGTGGGAGTTGTTCGGAGCTCTGGAAATG
AAGCAGAGCAAATTTACCAAGCGAATTTTCAGGATTTTCGACGAAGATGG
CAGCGGGTCCATCGACTTCCGAGAGTTCGTGATGAGTCTGTGGAACTACT
GCACCCTCTCCAAAGCGGCGTTGATAATGTTTGCGTTCGACCTGTACGAC
AACGACAACAGCGGCGAGATCGACATTGGCGAGGTCGAACTGCTGCTGAA
GGAGGTGTACGGGAGAGAGTTTCAGACTAGCTCCCAGGCGCAGGCAATTA
TGGCGAAGCTCAAGAGCGGAGAAAATGACATGAAGAGAGATATCAACGTA
GACAGCTTCCGAGAGTTTTGTCGGACCCACCCGGCCGTTTTGTACCCAGC
GTTCGTGTTGCAGACGCAGATGCAGAACAAGATACTGGGCTCTAAGTTTT
GGGCTTCGTGCGGCAAAAGCCGTGTGCAATTGTCCGACGGGAAGTACGTC
GACATCACAGATCTTCTGCAAGCTCACGTCAACCAAGATTTTCGACGAAG
ATGGCAGCGGGTCCATCGACTTCCGAGAGTTCGTGATGAGTCTGTGGAAC
TACTGCACCCTCTCCAAAGCGGCGTTGATAATGTTTGCGTTCGACCTGTA
CGACAACGACAACAGCGGCGAGATCGACATTGGCGAGGTCGAACTGCTGC
TGAAGGAGGTGTACGGGAGAGAGTTTCAGACTAGCTCCCAGGCGCAGGCA
ATTATGGCGAAGCTCAAGAGCGGAGAAAATGACATGAAGAGAGATATCAA
CGTAGACAGCTTCCGAGAGTTTTGTCGGACCCACCCGGCCGTTTTGTACC
CAGCGTTCGTGTTGCAGACGCAGATGCAGAACAAGATACTGGGCTCTAAG
TTTTGGGCTTCGTGCGGCAAAAGCCGTGTGCAATTGTCCGACGGGAAGTA
CGTCGACATCACAGATCTTCTGCAAGCTCACGTCAACCAA
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