mRNA_M-pyrifera_M_contig83376.19472.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig83376.19472.1
Unique NamemRNA_M-pyrifera_M_contig83376.19472.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Match: W9WL24_9EURO (Uncharacterized protein n=1 Tax=Cladophialophora psammophila CBS 110553 TaxID=1182543 RepID=W9WL24_9EURO)

HSP 1 Score: 70.1 bits (170), Expect = 4.720e-10
Identity = 76/268 (28.36%), Postives = 103/268 (38.43%), Query Frame = 1
Query:    7 PAVQEFIEAGADLEAVGEGGGTALH-AATVRHRLEIVKAXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXAVQRENGCTALHECAQRGMVEAVKALVENGAPLEAFDAAGDTPLLLAA---------IHDEENTLIFLAQNGADVAALRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPLILAXXXXXXXXXXXXXXXXXEVRVKRRESWR-------TALHEAVANGMTRAVELLLEKGADANEKD 759
            P V   I+ GADLEAV E G   LH AAT    LE +                                             ++  NG T LH  + R   EAVK L+E GA  +A D  G T L LA             +  T+ +L  NG     ++ +   T LH  +     EA++ L++ GA +DA D +  + L LA                  + +  R   R       T LH A + G TRAVELLL +GA+ +  D
Sbjct:  317 PMVDMVIQFGADLEAVDEMGCAPLHYAATGARSLETINMFSENPAFVR----------------------------------MKDHNGLTPLHIVSGRSWAEAVKHLLERGAEADAVDNDGRTALHLALNSTNKVSRYSRKDTRTIRYLCLNGPAQVNMKDKRSLTPLHIASGHSWAEAVELLLEQGAEVDAVDVDGRTALHLAANSHVNRHEHFDTRTIDLLSLDNRTQVRMRDKQGLTPLHLAASLGRTRAVELLLRRGAEVDAVD 550          
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Match: A0A7R8WN10_9CRUS (Hypothetical protein n=1 Tax=Cyprideis torosa TaxID=163714 RepID=A0A7R8WN10_9CRUS)

HSP 1 Score: 62.8 bits (151), Expect = 8.340e-8
Identity = 48/166 (28.92%), Postives = 76/166 (45.78%), Query Frame = 1
Query:  271 TALHECAQRGMVEAVKALVENGAPLEAFDAAGDTPLLLAAIHDEENTLIFLAQNGADVAALRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPLILAXXXXXXXXXXXXXXXXXEVRVKRRESWRTALHEAVANGMTRAVELLLEKGADANEKDREG 768
            T LH  A +   E  + L+E  A ++  D+ G TPLL A ++   + +  L  +GAD     RE   + LH+       E  + L+  GA ++A+DG+ D+PL+LA                 +  +  R    + LH A +       ELL+ KGA+ N  D +G
Sbjct:  139 TPLHLAATQ---ETARLLLEYKAKVDVKDSDGCTPLLHATVNGRHSVVELLLAHGADPNITNREEETSPLHQAKS---AETAELLIANGAVVNAKDGDGDTPLVLATENNRHSVVEVLLANRADPNIANRYGT-SPLHRAKS---AETAELLIAKGAEVNAMDEDG 294          
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Match: W3WVV4_PESFW (Uncharacterized protein n=1 Tax=Pestalotiopsis fici (strain W106-1 / CGMCC3.15140) TaxID=1229662 RepID=W3WVV4_PESFW)

HSP 1 Score: 63.5 bits (153), Expect = 8.500e-8
Identity = 42/109 (38.53%), Postives = 57/109 (52.29%), Query Frame = 1
Query:  271 TALHECAQRGMVEAVKALVENGAPLEAFDAAGDTPLLLAAI---HDEENTLIFLAQNGADVAALRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPLILA 588
            T LH     G +  V++LV  GA L   D+AG T L +A +   HD+    + L+  G D+  L  ENR+TALH  A+RG++E I  LV  GA     D    SP + A
Sbjct:  566 TKLHFAVHDGNLPLVESLVREGASLSNRDSAGRTALHVAVMKKSHDQAIMDLLLSAGGKDLVNLGDENRQTALHYAAERGLIETINILVSHGADTGTTDNFGFSPFLWA 674          
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Match: A0A2V8ZS14_9BACT (Uncharacterized protein n=1 Tax=Acidobacteria bacterium TaxID=1978231 RepID=A0A2V8ZS14_9BACT)

HSP 1 Score: 61.6 bits (148), Expect = 2.350e-7
Identity = 45/115 (39.13%), Postives = 58/115 (50.43%), Query Frame = 1
Query:  247 AVQRENGCTALHECAQRGMVEAVKALVENGAPLEAFDAAGDTPLLLAAIHDEENT----LIFLAQNGADVAALRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPL 579
            A +R  G T LH  A  G +E V  L+  GA          TPL   A      T    +  L + GADV A     R TALH  A+RG VE  +AL+ +GAA++ARD + D+PL
Sbjct:  212 ATRRFAGTTLLHFAAGAGCLEVVALLLRLGADPNVQGRGDHTPLYCVANECASETGPEVVRALVRAGADVNACGGVTRATALHMAARRGHVEIARALLDSGAAVNARDRKGDTPL 326          
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Match: A0A7J6ZNG7_9COLE (Uncharacterized protein n=1 Tax=Abscondita terminalis TaxID=2069292 RepID=A0A7J6ZNG7_9COLE)

HSP 1 Score: 60.8 bits (146), Expect = 7.400e-7
Identity = 48/168 (28.57%), Postives = 73/168 (43.45%), Query Frame = 1
Query:  259 ENGCTALHECAQRGMVEAVKALVENGAPLEAFDAAGDTPLLLAAIHDEENTLI-FLAQNGADVAALRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPLILAXXXXXXXXXXXXXXXXXEVRVKRRESWRTALHEAVANGMTRAVELLLEKGADANEKD 759
            ++G +ALH  A+ G    +  L+  GA +     +GDTPLLL  + +    ++  L  NGADV A+  E   + +H    +  ++ I  L KAGA I+ R+ + ++PL                                  H AV  G    V+LLL  GAD+N  D
Sbjct: 1352 KSGLSALHYAAKFGFENFLTELIRRGADVNQETKSGDTPLLLHVMENSRKGVVKLLIDNGADVCAVEAETGNSCMHYLVDKCEIDVIGDLAKAGADINVRNRDGNTPL----------------------------------HCAVRFGNCTLVKLLLSFGADSNVHD 1485          
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Match: A0A267GQ97_9PLAT (Uncharacterized protein n=5 Tax=Macrostomum lignano TaxID=282301 RepID=A0A267GQ97_9PLAT)

HSP 1 Score: 60.5 bits (145), Expect = 7.740e-7
Identity = 41/114 (35.96%), Postives = 62/114 (54.39%), Query Frame = 1
Query:  250 VQRENGCTALHECAQRGMVEAVKALVENGAPLEAFDAAGDTPLLLAAIHDEENTLIFLAQ-NGADVAALRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPLILA 588
            + R+  C ALH  +  G    V AL+E G      +  G+TPL +AA    E+ +  L    G  + A R+ +RRTALH  A  G  + ++ L++AGA ID RD + ++P+ LA
Sbjct:   42 LDRDGSC-ALHIGSYCGQEVGVHALLEFGCDPNVTNQNGETPLHVAAEQRFEHMIDLLVDAEGIRLDAARKLDRRTALHIAASHGCCDGVRRLLEAGAGIDVRDRDGNTPVFLA 154          
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Match: A0A7R8WJS6_9CRUS (Hypothetical protein n=1 Tax=Cyprideis torosa TaxID=163714 RepID=A0A7R8WJS6_9CRUS)

HSP 1 Score: 60.5 bits (145), Expect = 8.880e-7
Identity = 49/166 (29.52%), Postives = 78/166 (46.99%), Query Frame = 1
Query:  271 TALHECAQRGMVEAVKALVENGAPLEAFDAAGDTPLLLAAIHDEENTLIFLAQNGADVAALRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPLILAXXXXXXXXXXXXXXXXXEVRVKRRESWRTALHEAVANGMTRAVELLLEKGADANEKDREG 768
            T LH  A     E  + L+E  A ++  D+ G TPLL A ++   + +  L  +GAD     RE   + LH        E  + L++ GA ++A+D   ++PL +A                 + ++   E  R+ LHEA +    +  ELL++KGA+ N KDR G
Sbjct:  156 TPLHIAASP---ETARLLLEYKAKVDVKDSDGCTPLLQATLNGRHSVVELLLAHGADPNIANREYETSPLHHAES---AETAELLIQNGAEVNAKDICGNTPLFVATGYDCHTVVEVLLANGADPKIAN-EDERSPLHEARS---AKTAELLIQKGAEVNAKDRRG 311          
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Match: UPI0015C2DAF4 (Ankyrin repeat protein n=1 Tax=Lasiodiplodia theobromae TaxID=45133 RepID=UPI0015C2DAF4)

HSP 1 Score: 60.5 bits (145), Expect = 9.020e-7
Identity = 51/158 (32.28%), Postives = 78/158 (49.37%), Query Frame = 1
Query:  301 MVEAVKALVENGAPLE--AFDAAGDTPLLLAAIHDEENTLIFLAQNGADVAALRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPLILAXXXXXXXXXXXXXXXXXEVRVKRRESWRTALHEAVANGMTRAVELLLEKGADANEKDREG 768
            +V    AL+    P+   AFD++G T L+LAA  +E   + +L  NGADV  L  +NR +A++     G   A++ L+K G  +         P ILA                 ++ +   +   TA++EA   G+T AV+LL+EKGAD N  D +G
Sbjct:  433 IVSICNALLNGRQPINVNAFDSSGVTALMLAAELEELPVVRWLLNNGADVNMLDDQNR-SAIYYAVSAGSTTAVEELLKFGPNLACLHTSVKQP-ILATAIRNPQILEILLRTGVDLDLADADGC-TAINEASTQGVTDAVKLLIEKGADINHTDNQG 587          
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Match: UPI001D03E171 (ankyrin repeats (3 copies) domain-containing protein n=1 Tax=Hirsutella rhossiliensis TaxID=111463 RepID=UPI001D03E171)

HSP 1 Score: 59.7 bits (143), Expect = 1.020e-6
Identity = 40/113 (35.40%), Postives = 63/113 (55.75%), Query Frame = 1
Query:  250 VQRENGCTALHECAQRGMVEAVKALVENGAPLEAFDAAGDTPLLLAAIHDEENTLIFLAQNGADVAALRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPLILA 588
            V+ E+G T LH  A+ G +EAV+ L+    PL A D  G TPL +A +H +  T+  L QN AD   +R   + + + + A+ G    ++ L +AGAA +  + + +SP  LA
Sbjct:   62 VEEESGMTPLHYAAKCGRLEAVRFLLGKRFPLNATDKFGRTPLYMAVLHQQLETVKSLLQNDAD-PNIRCRCKTSTVSKAAEYGNRAMLRLLREAGAAWNTANVDGESPKELA 173          
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Match: UPI000C03AD2F (serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit A-like n=1 Tax=Stylophora pistillata TaxID=50429 RepID=UPI000C03AD2F)

HSP 1 Score: 59.3 bits (142), Expect = 1.380e-6
Identity = 44/160 (27.50%), Postives = 75/160 (46.88%), Query Frame = 1
Query:  289 AQRGMVEAVKALVENGAPLEAFDAAGDTPLLLAAIHDEENTLIFLAQNGADVAALRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPLILAXXXXXXXXXXXXXXXXXEVRVKRRESWRTALHEAVANGMTRAVELLLEKGADANEKDREG 768
            A+ G +  ++  +  G  + + +  G TPL +A ++ +  T+ +L +NGAD   ++    R  LH  +  G V  I  ++K G  ++++DG  D+PLILA                 +  +K     RTALH   A      +E+LL  G   + KD +G
Sbjct:    5 AKNGNITRLEKFLSLGRDVNSRNDNGVTPLRIAVLYGQSETVRYLLENGAD-PFVKGSYSRNLLHVASYGGQVAIIDEMIKQGLDVNSKDGNGDTPLILAAAYGREKAVKYLLQGGADPSIKGCYG-RTALH---AGNDVAIIEILLSSGVHIDTKDADG 159          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig83376.19472.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
W9WL24_9EURO4.720e-1028.36Uncharacterized protein n=1 Tax=Cladophialophora p... [more]
A0A7R8WN10_9CRUS8.340e-828.92Hypothetical protein n=1 Tax=Cyprideis torosa TaxI... [more]
W3WVV4_PESFW8.500e-838.53Uncharacterized protein n=1 Tax=Pestalotiopsis fic... [more]
A0A2V8ZS14_9BACT2.350e-739.13Uncharacterized protein n=1 Tax=Acidobacteria bact... [more]
A0A7J6ZNG7_9COLE7.400e-728.57Uncharacterized protein n=1 Tax=Abscondita termina... [more]
A0A267GQ97_9PLAT7.740e-735.96Uncharacterized protein n=5 Tax=Macrostomum lignan... [more]
A0A7R8WJS6_9CRUS8.880e-729.52Hypothetical protein n=1 Tax=Cyprideis torosa TaxI... [more]
UPI0015C2DAF49.020e-732.28Ankyrin repeat protein n=1 Tax=Lasiodiplodia theob... [more]
UPI001D03E1711.020e-635.40ankyrin repeats (3 copies) domain-containing prote... [more]
UPI000C03AD2F1.380e-627.50serine/threonine-protein phosphatase 6 regulatory ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig83376contigM-pyrifera_M_contig83376:14..781 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score73.6
Seed ortholog evalue1.5e-10
Seed eggNOG ortholog1182553.XP_007747179.1
Hectar predicted targeting categoryother localisation
EggNOG free text desc.ankyrin repeat protein
EggNOG OGs38BVK@33154,3NW9Q@4751,3QJDH@4890,COG0666@1,KOG4177@2759
COG Functional cat.M
Best tax levelAscomycota
Best eggNOG OGNA|NA|NA
Exons1
Model size768
Cds size768
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816740.7648711-CDS-M-pyrifera_M_contig83376:13..7811622816740.7648711-CDS-M-pyrifera_M_contig83376:13..781Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83376 14..781 +
1692278150.493619-CDS-M-pyrifera_M_contig83376:13..7811692278150.493619-CDS-M-pyrifera_M_contig83376:13..781Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83376 14..781 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig83376.19472.1prot_M-pyrifera_M_contig83376.19472.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig83376 14..781 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig83376.19472.1

>prot_M-pyrifera_M_contig83376.19472.1 ID=prot_M-pyrifera_M_contig83376.19472.1|Name=mRNA_M-pyrifera_M_contig83376.19472.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=256bp
DLPAVQEFIEAGADLEAVGEGGGTALHAATVRHRLEIVKALLEAGASLSA
TDTSGNTPLLVAAINEQEDFVKLFATDRASLTAVQRENGCTALHECAQRG
MVEAVKALVENGAPLEAFDAAGDTPLLLAAIHDEENTLIFLAQNGADVAA
LRRENRRTALHECAQRGMVEAIKALVKAGAAIDARDGERDSPLILAAQSG
KDEAVIALVEAGAEVRVKRRESWRTALHEAVANGMTRAVELLLEKGADAN
EKDREG
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mRNA from alignment at M-pyrifera_M_contig83376:14..781+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig83376.19472.1 ID=mRNA_M-pyrifera_M_contig83376.19472.1|Name=mRNA_M-pyrifera_M_contig83376.19472.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=768bp|location=Sequence derived from alignment at M-pyrifera_M_contig83376:14..781+ (Macrocystis pyrifera P11B4 male)
GATTTGCCGGCTGTGCAGGAGTTCATTGAGGCAGGAGCGGATCTTGAGGC AGTGGGGGAGGGAGGGGGGACGGCGTTGCATGCAGCAACAGTGCGACATC GATTGGAGATTGTCAAGGCTCTGTTGGAGGCTGGTGCTTCGTTGAGTGCT ACTGATACCAGTGGAAACACCCCGCTTCTTGTTGCTGCCATTAACGAACA AGAGGATTTCGTGAAGCTCTTTGCGACAGACAGGGCAAGTTTGACAGCAG TACAGCGAGAGAATGGGTGCACAGCACTCCACGAATGCGCACAGCGGGGC ATGGTTGAGGCAGTCAAGGCTTTGGTGGAGAATGGAGCTCCTTTGGAAGC CTTCGATGCAGCAGGCGACACCCCTCTCCTTCTCGCAGCCATCCATGATG AAGAAAACACCCTTATCTTCCTTGCTCAAAACGGAGCAGATGTGGCAGCA TTGCGACGAGAAAACAGGCGCACAGCACTCCACGAATGCGCACAGAGGGG TATGGTGGAGGCGATCAAGGCTTTGGTGAAGGCTGGGGCAGCGATTGATG CAAGAGATGGAGAACGTGACTCTCCGCTCATTCTCGCAGCTCAGAGTGGG AAAGATGAGGCTGTGATTGCTTTGGTGGAGGCTGGAGCGGAGGTGCGCGT GAAGCGACGAGAATCCTGGCGCACTGCACTGCACGAGGCAGTTGCAAATG GGATGACAAGAGCTGTCGAGTTGCTGCTGGAGAAAGGAGCAGATGCGAAC GAAAAAGACCGCGAGGGG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig83376:14..781+

>mRNA_M-pyrifera_M_contig83376.19472.1 ID=mRNA_M-pyrifera_M_contig83376.19472.1|Name=mRNA_M-pyrifera_M_contig83376.19472.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1536bp|location=Sequence derived from alignment at M-pyrifera_M_contig83376:14..781+ (Macrocystis pyrifera P11B4 male)
GATTTGCCGGCTGTGCAGGAGTTCATTGAGGCAGGAGCGGATCTTGAGGC
AGTGGGGGAGGGAGGGGGGACGGCGTTGCATGCAGCAACAGTGCGACATC
GATTGGAGATTGTCAAGGCTCTGTTGGAGGCTGGTGCTTCGTTGAGTGCT
ACTGATACCAGTGGAAACACCCCGCTTCTTGTTGCTGCCATTAACGAACA
AGAGGATTTCGTGAAGCTCTTTGCGACAGACAGGGCAAGTTTGACAGCAG
TACAGCGAGAGAATGGGTGCACAGCACTCCACGAATGCGCACAGCGGGGC
ATGGTTGAGGCAGTCAAGGCTTTGGTGGAGAATGGAGCTCCTTTGGAAGC
CTTCGATGCAGCAGGCGACACCCCTCTCCTTCTCGCAGCCATCCATGATG
AAGAAAACACCCTTATCTTCCTTGCTCAAAACGGAGCAGATGTGGCAGCA
TTGCGACGAGAAAACAGGCGCACAGCACTCCACGAATGCGCACAGAGGGG
TATGGTGGAGGCGATCAAGGCTTTGGTGAAGGCTGGGGCAGCGATTGATG
CAAGAGATGGAGAACGTGACTCTCCGCTCATTCTCGCAGCTCAGAGTGGG
AAAGATGAGGCTGTGATTGCTTTGGTGGAGGCTGGAGCGGAGGTGCGCGT
GAAGCGACGAGAATCCTGGCGCACTGCACTGCACGAGGCAGTTGCAAATG
GGATGACAAGAGCTGTCGAGTTGCTGCTGGAGAAAGGAGCAGATGCGAAC
GAAAAAGACCGCGAGGGGGATTTGCCGGCTGTGCAGGAGTTCATTGAGGC
AGGAGCGGATCTTGAGGCAGTGGGGGAGGGAGGGGGGACGGCGTTGCATG
CAGCAACAGTGCGACATCGATTGGAGATTGTCAAGGCTCTGTTGGAGGCT
GGTGCTTCGTTGAGTGCTACTGATACCAGTGGAAACACCCCGCTTCTTGT
TGCTGCCATTAACGAACAAGAGGATTTCGTGAAGCTCTTTGCGACAGACA
GGGCAAGTTTGACAGCAGTACAGCGAGAGAATGGGTGCACAGCACTCCAC
GAATGCGCACAGCGGGGCATGGTTGAGGCAGTCAAGGCTTTGGTGGAGAA
TGGAGCTCCTTTGGAAGCCTTCGATGCAGCAGGCGACACCCCTCTCCTTC
TCGCAGCCATCCATGATGAAGAAAACACCCTTATCTTCCTTGCTCAAAAC
GGAGCAGATGTGGCAGCATTGCGACGAGAAAACAGGCGCACAGCACTCCA
CGAATGCGCACAGAGGGGTATGGTGGAGGCGATCAAGGCTTTGGTGAAGG
CTGGGGCAGCGATTGATGCAAGAGATGGAGAACGTGACTCTCCGCTCATT
CTCGCAGCTCAGAGTGGGAAAGATGAGGCTGTGATTGCTTTGGTGGAGGC
TGGAGCGGAGGTGCGCGTGAAGCGACGAGAATCCTGGCGCACTGCACTGC
ACGAGGCAGTTGCAAATGGGATGACAAGAGCTGTCGAGTTGCTGCTGGAG
AAAGGAGCAGATGCGAACGAAAAAGACCGCGAGGGG
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