mRNA_M-pyrifera_M_contig83080.19406.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig83080.19406.1
Unique NamemRNA_M-pyrifera_M_contig83080.19406.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Match: A0A812UDS9_9DINO (GTE12 protein (Fragment) n=1 Tax=Symbiodinium sp. KB8 TaxID=230985 RepID=A0A812UDS9_9DINO)

HSP 1 Score: 199 bits (507), Expect = 2.670e-57
Identity = 92/156 (58.97%), Postives = 114/156 (73.08%), Query Frame = 1
Query:   55 LALHVHSLRAEAFVRKFSMVLMKVMESSLNGGVFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRLTFRNAMRYNPPGHRVHVAAHKLEEEFGSYLRRTRLRMQQDQTRRAKHVCGFCQGQVCGLCGEKCLRFESPSISC 522
            +A HVH+LRAE F R+F  +L +VME+ LNGGVFN PVD V LGIPDYP+IV+ PMDLGT+RQRLE+ RYT+      D+RL F NA+RYNP  H+VHV A K+ E+F   + R   +  QD  R+A H CGFC G VCGLCGEKCL+F+ P +SC
Sbjct:  132 IARHVHALRAEVFARRFRPLLARVMENPLNGGVFNSPVDPVRLGIPDYPTIVRNPMDLGTIRQRLEALRYTSTDSFAADIRLVFANAIRYNPATHQVHVTATKMREDFDRTITRHEAKADQDAARKASHSCGFCHGSVCGLCGEKCLKFDPPVLSC 287          
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Match: A0A5A8E306_CAFRO (Histone acetyltransferase n=8 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8E306_CAFRO)

HSP 1 Score: 199 bits (507), Expect = 4.790e-56
Identity = 92/156 (58.97%), Postives = 114/156 (73.08%), Query Frame = 1
Query:   55 LALHVHSLRAEAFVRKFSMVLMKVMESSLNGGVFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRLTFRNAMRYNPPGHRVHVAAHKLEEEFGSYLRRTRLRMQQDQTRRAKHVCGFCQGQVCGLCGEKCLRFESPSISC 522
            +A HVH+LRAE F R+F  +L +VME+ LNGGVFN PVD V LGIPDYP+IV+ PMDLGT+RQRLE+ RYT+      D+RL F NA+RYNP  H+VHV A K+ E+F   + R   +  QD  R+A H CGFC G VCGLCGEKCL+F+ P +SC
Sbjct:  589 IARHVHALRAEVFARRFRPLLARVMENPLNGGVFNSPVDPVRLGIPDYPTIVRNPMDLGTIRQRLEALRYTSTDSFAADIRLVFANAIRYNPATHQVHVTATKMREDFDRTITRHEAKADQDAARKASHSCGFCHGSVCGLCGEKCLKFDPPVLSC 744          
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Match: A0A7S3YAC7_HETAK (Hypothetical protein (Fragment) n=1 Tax=Heterosigma akashiwo TaxID=2829 RepID=A0A7S3YAC7_HETAK)

HSP 1 Score: 143 bits (360), Expect = 6.080e-39
Identity = 73/168 (43.45%), Postives = 101/168 (60.12%), Query Frame = 1
Query:   19 SLAATDALRSSQLALHVHSLRAEAFVRKFSMVLMKVMESSLNGGVFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRLTFRNAMRYNPPGHRVHVAAHKLEEEFGSYLRRTRLRMQQDQTRRAKHVCGFCQGQVCGLCGEKCLRFESPSISC 522
            +L+A D   +  +  HV  +RAE F       L ++++  LN GVFN+PVD VALG+P Y  +V+ P+DLGTV+ RL    Y   +    DVRL F NA+ +NP GH V  AA  L  EF + L R   ++++D  R  +H CG C G+ CGLCG+KCLR E P ++C
Sbjct:    1 ALSALDGAGARAVYAHVVGVRAEHFHAYLRAPLQRLLQHPLNRGVFNRPVDPVALGLPTYREVVRRPLDLGTVKTRLLRFEYPGVAAFAADVRLVFANALAFNPAGHPVAKAARALGGEFEAELARAADKVRRDHARAGRHACGRCAGRSCGLCGDKCLRLEPPLVTC 168          
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Match: A0A7S2C9K0_9STRA (Histone acetyltransferase (Fragment) n=1 Tax=Dictyocha speculum TaxID=35687 RepID=A0A7S2C9K0_9STRA)

HSP 1 Score: 142 bits (357), Expect = 6.770e-36
Identity = 70/153 (45.75%), Postives = 96/153 (62.75%), Query Frame = 1
Query:   64 HVHSLRAEAFVRKFSMVLMKVMESSLNGGVFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRLTFRNAMRYNPPGHRVHVAAHKLEEEFGSYLRRTRLRMQQDQTRRAKHVCGFCQGQVCGLCGEKCLRFESPSISC 522
            HV  LR E  +R    +L ++M  +LN G+FN+PVD VALG+PDY  IVK PMD GT++ +L +  Y    +   DVRL   NAM YNP  ++VH+ A +L EEF   L +   ++ +D  RRA+H CG CQG+ C LCGEKCL+ +   + C
Sbjct:  273 HVAMLRTEPVLRVARPILQRIMAHNLNRGLFNEPVDAVALGLPDYHRIVKRPMDFGTMKDQLYALHYERLEQFIADVRLVLSNAMVYNPSTNQVHINARQLLEEFEDELGKLGKKISRDTKRRAQHNCGVCQGRTCRLCGEKCLKLDPVMLIC 425          
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Match: W4GA57_9STRA (Histone acetyltransferase n=11 Tax=Aphanomyces astaci TaxID=112090 RepID=W4GA57_9STRA)

HSP 1 Score: 136 bits (343), Expect = 4.600e-34
Identity = 70/167 (41.92%), Postives = 95/167 (56.89%), Query Frame = 1
Query:   34 DALRSSQLALHVHSLRAEAFVRKFSMVLMKVMESSLNGGVFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRLTFRNAMRYNPPGHRVHVAA----HKLEEEFGSYLRRTRLRMQQDQTRRAKHVCGFCQGQVCGLCGEKCLRFESPSISC 522
            D   ++QL  H+  LR E        V+ ++M   +N   FN PVD V L  P Y  ++KTPMDLGT++QRL S  Y NP +   D+RL F+NAM +NPPGH VH AA     + E E+ ++LR+   R+     +R  H C  CQ  VC LC EKC+ F+ P + C
Sbjct:   52 DTASAAQLRQHLQDLRREQMAVPMLGVVTRLMIHRINQSFFNTPVDVVRLACPTYYDVIKTPMDLGTIKQRLGSLEYANPDDAANDIRLVFQNAMLFNPPGHVVHDAAAILLKEFEREYVAFLRKQADRLD----KRNAHSCPHCQSHVCALCNEKCINFDPPLVMC 214          
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Match: A0A7S1U9I3_9STRA (Histone acetyltransferase (Fragment) n=1 Tax=Phaeomonas parva TaxID=124430 RepID=A0A7S1U9I3_9STRA)

HSP 1 Score: 136 bits (343), Expect = 4.970e-34
Identity = 71/168 (42.26%), Postives = 94/168 (55.95%), Query Frame = 1
Query:   19 SLAATDALRSSQLALHVHSLRAEAFVRKFSMVLMKVMESSLNGGVFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRLTFRNAMRYNPPGHRVHVAAHKLEEEFGSYLRRTRLRMQQDQTRRAKHVCGFCQGQVCGLCGEKCLRFESPSISC 522
            S++  + L    L  H+  LR E   R    +L K+M  + N G FN PVD VAL +PDY  I+  PMDLGT++ RL+S  Y +      DVRLTF NAMRYNP  + VH AA  L  EF     +   ++ ++  RR KH C  C G  C +CG+KCL+FE   + C
Sbjct:  224 SISPLEQLDRLALEAHIVGLRREGMQRSLKAILSKIMLHTANRGTFNAPVDSVALRLPDYTRIITKPMDLGTIKLRLQSLAYRDMDSFAADVRLTFGNAMRYNPAKNNVHEAAKTLLGEFEHEFAKLLSKLDKEDGRREKHECEHCHGHTCVVCGDKCLKFEPAVLIC 391          
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Match: D8M6J5_BLAHO (Histone acetyltransferase n=1 Tax=Blastocystis hominis TaxID=12968 RepID=D8M6J5_BLAHO)

HSP 1 Score: 131 bits (330), Expect = 2.270e-32
Identity = 62/153 (40.52%), Postives = 92/153 (60.13%), Query Frame = 1
Query:   64 HVHSLRAEAFVRKFSMVLMKVMESSLNGGVFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRLTFRNAMRYNPPGHRVHVAAHKLEEEFGSYLRRTRLRMQQDQTRRAKHVCGFCQGQVCGLCGEKCLRFESPSISC 522
            +V  L+       F+ ++  +M+   N G+FN PVD     +P Y SI+K PMDLGTVR RL SG YT+ SE+ +D+ L FRNA ++NP  H +++ A  L + F S   + + R++Q++  +A H C  C+G  C +CGEKCLR+  P   C
Sbjct:    9 YVSFLKTRNLQAYFAPLIQLLMQHKDNHGIFNTPVDPDVQNLPTYYSIIKKPMDLGTVRDRLASGYYTSQSEIMDDISLVFRNAQKFNPAPHFIYLCASSLSKVFESEAAKIQTRIEQNRVNQANHFCSSCRGTPCRICGEKCLRYSPPVFVC 161          
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Match: A0A835ZGH1_9STRA (Histone acetyltransferase (Fragment) n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZGH1_9STRA)

HSP 1 Score: 131 bits (329), Expect = 3.470e-32
Identity = 68/169 (40.24%), Postives = 94/169 (55.62%), Query Frame = 1
Query:   19 SLAATDALRSSQLALHVHSLRAEAFVRKFSMVLMKVMESSLN-GGVFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRLTFRNAMRYNPPGHRVHVAAHKLEEEFGSYLRRTRLRMQQDQTRRAKHVCGFCQGQVCGLCGEKCLRFESPSISC 522
            SL+A        L  H+ +LR +  +R    VL ++M  + N  G+FN PVD VAL IP+Y  ++  PMD GTV+ RL S  Y        D RL   NA+ +NPP H VH  A  L+ EF +   + + R+ +D  ++ +H C  CQGQ C LCG+KCLR E P + C
Sbjct:    8 SLSALSVATPEDLVSHLQALRRDFILRVLRPVLNRLMSHTSNRNGLFNTPVDPVALNIPNYLDVISQPMDFGTVKCRLFSLEYDEADAFAADARLVLSNAITFNPPSHPVHQCAVALQAEFDAEYSKAQTRLVRDAQKKDEHHCTLCQGQTCALCGDKCLRLEPPVLIC 176          
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Match: A0A7R9U6P2_9STRA (Histone acetyltransferase n=1 Tax=Pinguiococcus pyrenoidosus TaxID=172671 RepID=A0A7R9U6P2_9STRA)

HSP 1 Score: 130 bits (326), Expect = 9.740e-32
Identity = 64/127 (50.39%), Postives = 79/127 (62.20%), Query Frame = 1
Query:  142 NGGVFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRLTFRNAMRYNPPGHRVHVAAHKLEEEFGSYLRRTRLRMQQDQTRRAKHVCGFCQGQVCGLCGEKCLRFESPSISC 522
            N G FN PVD VAL +PDY  ++  P+DLGT++QRL +  Y       EDVRL FRNAMRYNP  + VH AA  L  EF     +   +M ++  RRAKH C  CQGQ C +CGEKCL+FE   + C
Sbjct:    1 NKGTFNSPVDPVALNLPDYFRVIPKPLDLGTIKQRLNALLYREVDTFAEDVRLVFRNAMRYNPAKNVVHEAAKTLLHEFEHDFSKMMSKMDKEDGRRAKHECPHCQGQTCVVCGEKCLKFEPAVLIC 127          
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Match: A0A7S1CRJ4_9STRA (Hypothetical protein (Fragment) n=1 Tax=Bicosoecida sp. CB-2014 TaxID=1486930 RepID=A0A7S1CRJ4_9STRA)

HSP 1 Score: 119 bits (298), Expect = 2.600e-31
Identity = 59/114 (51.75%), Postives = 75/114 (65.79%), Query Frame = 1
Query:   10 VDRSLAATDALRSSQLALHVHSLRAEAFVRKFSMVLMKVMESSLNGGVFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRLTFRNAMRYNPPGHRVHV 351
            +  SL+  DA    QL  H+  LRA+ F+R+F  +L + +E   N GVFN PVD VAL + DY  ++K PMDLGT+RQRLE G YT   +L +DVRL F NA  YNPPGH +HV
Sbjct:   30 IKASLSPIDAFSKEQLDHHITILRADTFLRRFRPLLTRFVEHPANAGVFNDPVDPVALNLVDYHRVIKHPMDLGTIRQRLECGWYTTADDLADDVRLVFTNARIYNPPGHPIHV 143          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig83080.19406.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A812UDS9_9DINO2.670e-5758.97GTE12 protein (Fragment) n=1 Tax=Symbiodinium sp. ... [more]
A0A5A8E306_CAFRO4.790e-5658.97Histone acetyltransferase n=8 Tax=Cafeteria roenbe... [more]
A0A7S3YAC7_HETAK6.080e-3943.45Hypothetical protein (Fragment) n=1 Tax=Heterosigm... [more]
A0A7S2C9K0_9STRA6.770e-3645.75Histone acetyltransferase (Fragment) n=1 Tax=Dicty... [more]
W4GA57_9STRA4.600e-3441.92Histone acetyltransferase n=11 Tax=Aphanomyces ast... [more]
A0A7S1U9I3_9STRA4.970e-3442.26Histone acetyltransferase (Fragment) n=1 Tax=Phaeo... [more]
D8M6J5_BLAHO2.270e-3240.52Histone acetyltransferase n=1 Tax=Blastocystis hom... [more]
A0A835ZGH1_9STRA3.470e-3240.24Histone acetyltransferase (Fragment) n=1 Tax=Tribo... [more]
A0A7R9U6P2_9STRA9.740e-3250.39Histone acetyltransferase n=1 Tax=Pinguiococcus py... [more]
A0A7S1CRJ4_9STRA2.600e-3151.75Hypothetical protein (Fragment) n=1 Tax=Bicosoecid... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig83080contigM-pyrifera_M_contig83080:379..900 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score135.2
Seed ortholog evalue2.9e-29
Seed eggNOG ortholog2880.D7FK57
KEGG koko:K04498,ko:K11684,ko:K22261
KEGG Pathwayko04024,ko04066,ko04068,ko04110,ko04310,ko04330,ko04350,ko04520,ko04630,ko04720,ko04916,ko04919,ko04922,ko05016,ko05152,ko05161,ko05164,ko05165,ko05166,ko05167,ko05168,ko05169,ko05200,ko05203,ko05206,ko05211,ko05215,map04024,map04066,map04068,map04110,map04310,map04330,map04350,map04520,map04630,map04720,map04916,map04919,map04922,map05016,map05152,map05161,map05164,map05165,map05166,map05167,map05168,map05169,map05200,map05203,map05206,map05211,map05215
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006914,GO:0008150,GO:0008152,GO:0009056,GO:0009987,GO:0030674,GO:0043621,GO:0044237,GO:0044248,GO:0044424,GO:0044464,GO:0060090,GO:0061919
EggNOG free text desc.histone acetyltransferase activity
EggNOG OGsCOG5076@1,KOG1474@2759,KOG1778@2759
EC2.3.1.48
COG Functional cat.BK
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03000,ko03029,ko03036,ko04131
Exons1
Model size522
Cds size522
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816737.737339-CDS-M-pyrifera_M_contig83080:378..9001622816737.737339-CDS-M-pyrifera_M_contig83080:378..900Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83080 379..900 +
1692278148.96902-CDS-M-pyrifera_M_contig83080:378..9001692278148.96902-CDS-M-pyrifera_M_contig83080:378..900Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83080 379..900 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig83080.19406.1prot_M-pyrifera_M_contig83080.19406.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig83080 379..900 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig83080.19406.1

>prot_M-pyrifera_M_contig83080.19406.1 ID=prot_M-pyrifera_M_contig83080.19406.1|Name=mRNA_M-pyrifera_M_contig83080.19406.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=174bp
MLSVDRSLAATDALRSSQLALHVHSLRAEAFVRKFSMVLMKVMESSLNGG
VFNQPVDYVALGIPDYPSIVKTPMDLGTVRQRLESGRYTNPSELEEDVRL
TFRNAMRYNPPGHRVHVAAHKLEEEFGSYLRRTRLRMQQDQTRRAKHVCG
FCQGQVCGLCGEKCLRFESPSISC
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mRNA from alignment at M-pyrifera_M_contig83080:379..900+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig83080.19406.1 ID=mRNA_M-pyrifera_M_contig83080.19406.1|Name=mRNA_M-pyrifera_M_contig83080.19406.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=522bp|location=Sequence derived from alignment at M-pyrifera_M_contig83080:379..900+ (Macrocystis pyrifera P11B4 male)
ATGTTATCGGTAGATCGAAGTCTTGCTGCGACAGATGCTTTGCGATCGAG TCAGCTTGCACTCCATGTGCATTCGCTGCGTGCAGAAGCATTTGTACGCA AGTTTTCGATGGTGTTGATGAAGGTGATGGAATCTTCTCTGAATGGTGGT GTTTTTAATCAACCAGTAGACTATGTTGCACTTGGAATTCCTGATTATCC ATCGATTGTGAAAACACCCATGGATTTGGGAACTGTTCGTCAAAGACTCG AGTCTGGAAGGTACACAAACCCTTCCGAATTGGAGGAAGACGTACGTCTG ACATTTCGCAATGCGATGCGATACAATCCACCAGGGCATCGAGTACACGT TGCTGCTCACAAATTGGAAGAAGAATTTGGGAGTTACTTGCGACGCACAC GGCTTCGGATGCAGCAAGATCAGACACGTCGTGCGAAGCACGTTTGTGGG TTTTGTCAAGGACAAGTTTGTGGGTTGTGTGGTGAAAAGTGTTTGCGGTT TGAGTCACCATCAATCTCTTGT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig83080:379..900+

>mRNA_M-pyrifera_M_contig83080.19406.1 ID=mRNA_M-pyrifera_M_contig83080.19406.1|Name=mRNA_M-pyrifera_M_contig83080.19406.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1044bp|location=Sequence derived from alignment at M-pyrifera_M_contig83080:379..900+ (Macrocystis pyrifera P11B4 male)
ATGTTATCGGTAGATCGAAGTCTTGCTGCGACAGATGCTTTGCGATCGAG
TCAGCTTGCACTCCATGTGCATTCGCTGCGTGCAGAAGCATTTGTACGCA
AGTTTTCGATGGTGTTGATGAAGGTGATGGAATCTTCTCTGAATGGTGGT
GTTTTTAATCAACCAGTAGACTATGTTGCACTTGGAATTCCTGATTATCC
ATCGATTGTGAAAACACCCATGGATTTGGGAACTGTTCGTCAAAGACTCG
AGTCTGGAAGGTACACAAACCCTTCCGAATTGGAGGAAGACGTACGTCTG
ACATTTCGCAATGCGATGCGATACAATCCACCAGGGCATCGAGTACACGT
TGCTGCTCACAAATTGGAAGAAGAATTTGGGAGTTACTTGCGACGCACAC
GGCTTCGGATGCAGCAAGATCAGACACGTCGTGCGAAGCACGTTTGTGGG
TTTTGTCAAGGACAAGTTTGTGGGTTGTGTGGTGAAAAGTGTTTGCGGTT
TGAGTCACCATCAATCTCTTGTATGTTATCGGTAGATCGAAGTCTTGCTG
CGACAGATGCTTTGCGATCGAGTCAGCTTGCACTCCATGTGCATTCGCTG
CGTGCAGAAGCATTTGTACGCAAGTTTTCGATGGTGTTGATGAAGGTGAT
GGAATCTTCTCTGAATGGTGGTGTTTTTAATCAACCAGTAGACTATGTTG
CACTTGGAATTCCTGATTATCCATCGATTGTGAAAACACCCATGGATTTG
GGAACTGTTCGTCAAAGACTCGAGTCTGGAAGGTACACAAACCCTTCCGA
ATTGGAGGAAGACGTACGTCTGACATTTCGCAATGCGATGCGATACAATC
CACCAGGGCATCGAGTACACGTTGCTGCTCACAAATTGGAAGAAGAATTT
GGGAGTTACTTGCGACGCACACGGCTTCGGATGCAGCAAGATCAGACACG
TCGTGCGAAGCACGTTTGTGGGTTTTGTCAAGGACAAGTTTGTGGGTTGT
GTGGTGAAAAGTGTTTGCGGTTTGAGTCACCATCAATCTCTTGT
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