mRNA_M-pyrifera_M_contig83022.19396.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig83022.19396.1
Unique NamemRNA_M-pyrifera_M_contig83022.19396.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Match: A0A7Z9Y9T2_9BACT (Uncharacterized protein n=1 Tax=Candidatus Poribacteria bacterium TaxID=2026781 RepID=A0A7Z9Y9T2_9BACT)

HSP 1 Score: 95.9 bits (237), Expect = 1.320e-19
Identity = 66/203 (32.51%), Postives = 104/203 (51.23%), Query Frame = 1
Query:    1 DGHNNHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNSFGHNVVSVVGEKQGKKGTGVVESFASQSTTPEATSLGDNVASVTINATNAYVAGGALEMKRTITAGPNWSWVDVVDTVHHPRAKRFDWRFHTFANVSTDTNGVVFLSQDGKKIRVNATTAPSYPLIWNVTQPTFAPPDYPGDGLTAISFHVPN 609
            D   NH HLD+ SF L+  G RW +D G  +YN+ GYFG+QR+++YRL S GHN +++ GE Q  +    +  F S   TP+       +AS T + ++AY    A ++ RTI+      +V + D +   +  +  W  HT A+V  + + VV LSQ G ++ +      +   +    + +  PP  P  G+  I  H PN
Sbjct:  191 DNKANHSHLDLGSFVLDADGHRWALDLGSDNYNIPGYFGRQRWSYYRLRSEGHNTITLNGENQSPRAKAPILDFKS---TPD-------LASATADLSDAY-RDLAEKVLRTISLVER-RYVLIRDEIKARKPVKVVWGMHTPAHVKVEGSKVV-LSQGGSELLLEVLEPKN--AVVEAHEVSVPPPQRPIKGIRKIVVHPPN 378          
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Match: A0A7C2ZTR1_9BACT (Uncharacterized protein n=1 Tax=Candidatus Poribacteria bacterium TaxID=2026781 RepID=A0A7C2ZTR1_9BACT)

HSP 1 Score: 85.1 bits (209), Expect = 6.730e-16
Identity = 61/203 (30.05%), Postives = 96/203 (47.29%), Query Frame = 1
Query:    1 DGHNNHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNSFGHNVVSVVGEKQGKKGTGVVESFASQSTTPEATSLGDNVASVTINATNAYVAGGALEMKRTITAGPNWSWVDVVDTVHHPRAKRFDWRFHTFANVSTDTNGVVFLSQDGKKIRVNATTAPSYPLIWNVTQPTFAPPDYPGDGLTAISFHVPN 609
            D   NH HLD+ SF L+  G RW VD G   YN+ GYFG+QR+++YRL + GHN +++ GE Q       +  F S   TP+       +AS   + + AY    A ++ RT++      +V + D +         W  HT A+V  + +  + LSQ+G  + +          I    + +  PP  P  G+  I+   P+
Sbjct:  140 DNKANHSHLDLGSFVLDADGHRWAVDLGADDYNIPGYFGRQRWSYYRLRTEGHNTITLNGENQDPHAEAPIVDFKS---TPD-------LASAAADLSAAY-GDLAEKVLRTVSLVER-RYVLIRDVIKAREPVEVAWNMHTLASVKVEGSKAM-LSQNGATLLLEVIEPKD--AIVESHEVSIPPPQRPIKGIRKITVRPPS 327          
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Match: A0A7C2CZY7_9BACT (Coagulation factor 5/8 type domain-containing protein n=1 Tax=Armatimonadetes bacterium TaxID=2033014 RepID=A0A7C2CZY7_9BACT)

HSP 1 Score: 83.6 bits (205), Expect = 5.500e-15
Identity = 60/173 (34.68%), Postives = 78/173 (45.09%), Query Frame = 1
Query:    1 DGHNNHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNSFGHNVVSVVGEK---QGKKGTGVVESFASQSTTPEATSLGDNVASVTINATNAYVAGGALEMKRTITAGPNWSWVDVVDTVHHPRAKRFDWRFHTFANVSTDTNGVVFLSQDGKKIRVNATTA 510
            D   NH HLD+ +F L+  G RW VD G  +YNL GYFG+QR+ +YRL + GHN + +   +   Q       +  F SQ           N A    + TNAY   GA ++ R I        V V D V   +A    W  HT A V         LS+DGK   +   TA
Sbjct:  384 DNKFNHSHLDIGTFVLDAMGHRWAVDLGADNYNLPGYFGRQRWTYYRLRAEGHNTLVINPSQEPDQDPSAEAKIVRFVSQ----------PNRAFAIADLTNAYAKHGAEKVMRGILLDRKRREVVVQDEVTLKQAGDVWWFMHTPAQVQ--------LSEDGKTATLTQGTA 538          
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Match: A0A7C4IRF5_9BACT (Uncharacterized protein n=1 Tax=Planctomycetes bacterium TaxID=2026780 RepID=A0A7C4IRF5_9BACT)

HSP 1 Score: 82.4 bits (202), Expect = 6.410e-15
Identity = 68/218 (31.19%), Postives = 99/218 (45.41%), Query Frame = 1
Query:    1 DGHNNHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNSFGHNVVSVVGEKQGKKGTGVVESFASQSTTPEATSLGDNVASVTINATNAYVAGGALEMKRTITAGPNWSWVDVVDTVHHPRAKRFDWRFHTFANVSTDTNGVVFLSQDGKKIRVNATTAPSYPLIWNVTQPTFAP-PDYPGDGLTAISFHVPNPPTSTLSIRVTFAP 651
            D   NH HL++  F L+  GQRW VD G   YNL GYFGKQR+ +YRL + G N + + G  Q  +    + +F S+   PE      ++++       A   G AL  +R +        VD +DTV         W+ HT A V+ +  G   LS    ++R     AP+       T    AP P    DG+T +   +P  P  T ++ + F P
Sbjct:  122 DNKANHSHLELGMFVLDADGQRWAVDLGPDDYNLPGYFGKQRWTYYRLATEGQNTLLIGGRNQDPRAVAPIIAFQSK---PEWAFAVADLSAAYQGQAKAVARGAALRDRRQVLIQDEI--VDPMDTVV--------WQMHTPAKVTVE-GGTAQLSIKDARLRAEI-VAPAGAKF--ETASANAPSPQRSNDGITKLVVRLPRRPGKT-TLAIAFTP 321          
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Match: A0A7G9NW55_9BACT (DUF4962 domain-containing protein n=1 Tax=Planctomycetales bacterium zrk34 TaxID=2723666 RepID=A0A7G9NW55_9BACT)

HSP 1 Score: 82.8 bits (203), Expect = 1.010e-14
Identity = 57/165 (34.55%), Postives = 81/165 (49.09%), Query Frame = 1
Query:   13 NHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNSFGHNVVSVVGEK---QGKKGTGVVESFASQSTTPEATSLGDNVASVTINATNAYVAGGALEMKRTITAGPNWSWVDVVDTVHHPRAKRFDWRFHTFANVSTD-TNGVVFLSQDGKKIRV 495
            NH HLD+ SF L+  G+RW+ D G  +YNL  YFG+ R+N+YRL + GHN + +  +    Q  K T  +  F S          GD+ A   ++ T AY A    + KR +        V V D V   +  +  W  HT A V+ D    +  L+Q  K+IRV
Sbjct:  385 NHSHLDLGSFILDAHGERWINDLGADNYNLPSYFGRGRWNYYRLRAEGHNTLVIAPDNKADQNPKATAPITRFKS----------GDDHALAIVDLTPAY-APRVKQAKRGLMLMNQRRSVLVQDEVECEKPTQVLWFMHTPAKVALDEARRIATLNQHDKQIRV 538          
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Match: A0A7C4EDX0_9BACT (DUF4962 domain-containing protein n=1 Tax=Phycisphaerae bacterium TaxID=2026778 RepID=A0A7C4EDX0_9BACT)

HSP 1 Score: 82.4 bits (202), Expect = 1.380e-14
Identity = 57/163 (34.97%), Postives = 78/163 (47.85%), Query Frame = 1
Query:    1 DGHNNHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNSFGHNVVSVVGEKQGKKGTGVVESFASQSTTPEATSLGDNVASVTINATNAYVAGGALEMKRTITAGPNWSWVDVVDTVHHPRAKRFDWRFHTFANVSTDTNGVVFLSQDGKKI 489
            D   NH HLD+ +F L+  GQRW VD G   YNL GYFGK R+ +YRL + GHN + + GE Q   G   + +F S      A +   +++S    A  + + G AL  +         S V V D V   +     W+ HT A V+ D      LS  GK +
Sbjct:  407 DNKANHSHLDLGTFVLDALGQRWAVDLGGDDYNLPGYFGKDRWTYYRLRTEGHNTLVIDGENQATDGKAPIVAFRSADALAYAVA---DMSSGYRTAATSVLRGVALHDR---------SIVLVQDEVKAGKPVEVVWQMHTPAKVAIDGTTAT-LSLGGKTL 556          
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Match: UPI0013EACFF6 (heparinase II/III family protein n=1 Tax=unclassified Paludisphaera TaxID=2624067 RepID=UPI0013EACFF6)

HSP 1 Score: 81.6 bits (200), Expect = 2.530e-14
Identity = 55/163 (33.74%), Postives = 75/163 (46.01%), Query Frame = 1
Query:    1 DGHNNHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNSFGHNVVSVVGEKQGKKGTGVVESFASQSTTPEATSLGDNVASVTINATNAYVAGGALEMKRTITAGPNWSWVDVVDTVHHPRAKRFDWRFHTFANVSTDTNGVVFLSQDGKKI 489
            D    H HLD+ SF L+ QG+RW VD G   YNL GYFGK R+++YRL +  HN + +    Q  K    +  F S   TPE    G  VA +T     AY    +   +     G     V + D +   R  +  W FHT A+      G   LS+  +K+
Sbjct:  397 DNKAGHSHLDLGSFVLDAQGERWAVDLGSDDYNLPGYFGKNRWDYYRLRTESHNTLLIDDANQDPKAEAPLVRFLS---TPE---RGHAVADLTA----AYAPTVSKAWRGVARLGSEG--VLIQDEIEAARPVKLKWNFHTDASKIQTEGGQATLSKGDRKL 547          
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Match: A0A7C4VEE6_9BACT (DUF4962 domain-containing protein n=1 Tax=Armatimonadetes bacterium TaxID=2033014 RepID=A0A7C4VEE6_9BACT)

HSP 1 Score: 81.3 bits (199), Expect = 3.430e-14
Identity = 37/76 (48.68%), Postives = 48/76 (63.16%), Query Frame = 1
Query:    1 DGHNNHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNSFGHNVVSVVGEKQGKKGTGVVESFAS 228
            D   NH HLD+ SF L+  G+RW VD G   Y+L GYFGKQR+ +YRL + GHN +SV GE Q       + +F+S
Sbjct:  391 DNKANHSHLDLGSFVLDALGKRWAVDLGPDDYDLPGYFGKQRWTYYRLRTEGHNTISVNGENQDPSADAPIIAFSS 466          
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Match: A0A847GK96_9BACT (DUF4962 domain-containing protein n=1 Tax=Candidatus Anammoximicrobium sp. TaxID=2699742 RepID=A0A847GK96_9BACT)

HSP 1 Score: 79.7 bits (195), Expect = 1.160e-13
Identity = 54/146 (36.99%), Postives = 78/146 (53.42%), Query Frame = 1
Query:   13 NHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNSFGHNVVSV---VGEKQGKKGTGVVESFASQSTTPEATSLGDNVASVTINATNAYVAGGALEMKRTITAGPNWSWVDVVDTVHHPRAKRFDWRFHTFANVS 441
            NH HLD+ +F LE  GQRW VD G  +YNL GYFG+QR+++YRL + GHN + V    G  Q  +    +  F       EA   G + ++V ++ T AY A  A +++RTI       WV + D +  P       + HT A++S
Sbjct:  402 NHSHLDLGTFVLEALGQRWFVDLGADNYNLPGYFGRQRWDYYRLRAEGHNTLLVNPGKGPDQDPRAATKIVRF-------EAPGEGGSPSAV-LDLTPAY-AMHATQVQRTICLHDR-RWVTIRDEIQTPAPAEIWSQLHTPADIS 537          
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Match: A0A2D5SAA4_9PLAN (DUF4962 domain-containing protein n=1 Tax=Planctomycetaceae bacterium TaxID=2026779 RepID=A0A2D5SAA4_9PLAN)

HSP 1 Score: 79.3 bits (194), Expect = 1.600e-13
Identity = 35/76 (46.05%), Postives = 49/76 (64.47%), Query Frame = 1
Query:    1 DGHNNHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNSFGHNVVSVVGEKQGKKGTGVVESFAS 228
            D   NHGHLD+ SF L+  G RW  D G  +YNL+GYFG+QR+++YRLN+  HN + V  + Q  K    + +F+S
Sbjct:  429 DNQTNHGHLDIGSFVLDAGGVRWATDLGSDNYNLRGYFGRQRWSYYRLNNRSHNTLVVDDKLQNSKAVCRIVAFSS 504          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig83022.19396.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7Z9Y9T2_9BACT1.320e-1932.51Uncharacterized protein n=1 Tax=Candidatus Poribac... [more]
A0A7C2ZTR1_9BACT6.730e-1630.05Uncharacterized protein n=1 Tax=Candidatus Poribac... [more]
A0A7C2CZY7_9BACT5.500e-1534.68Coagulation factor 5/8 type domain-containing prot... [more]
A0A7C4IRF5_9BACT6.410e-1531.19Uncharacterized protein n=1 Tax=Planctomycetes bac... [more]
A0A7G9NW55_9BACT1.010e-1434.55DUF4962 domain-containing protein n=1 Tax=Planctom... [more]
A0A7C4EDX0_9BACT1.380e-1434.97DUF4962 domain-containing protein n=1 Tax=Phycisph... [more]
UPI0013EACFF62.530e-1433.74heparinase II/III family protein n=1 Tax=unclassif... [more]
A0A7C4VEE6_9BACT3.430e-1448.68DUF4962 domain-containing protein n=1 Tax=Armatimo... [more]
A0A847GK96_9BACT1.160e-1336.99DUF4962 domain-containing protein n=1 Tax=Candidat... [more]
A0A2D5SAA4_9PLAN1.600e-1346.05DUF4962 domain-containing protein n=1 Tax=Planctom... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig83022contigM-pyrifera_M_contig83022:35..697 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score78.6
Seed ortholog evalue4.2e-12
Seed eggNOG ortholog794903.OPIT5_14565
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Heparinase II/III-like protein
EggNOG OGs3K8X7@414999,46V2D@74201,COG4225@1,COG4225@2
COG Functional cat.S
Best tax levelOpitutae
Best eggNOG OGNA|NA|NA
Exons1
Model size663
Cds size663
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816737.3447325-CDS-M-pyrifera_M_contig83022:34..6971622816737.3447325-CDS-M-pyrifera_M_contig83022:34..697Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83022 35..697 +
1692278148.800581-CDS-M-pyrifera_M_contig83022:34..6971692278148.800581-CDS-M-pyrifera_M_contig83022:34..697Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig83022 35..697 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig83022.19396.1prot_M-pyrifera_M_contig83022.19396.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig83022 35..697 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig83022.19396.1

>prot_M-pyrifera_M_contig83022.19396.1 ID=prot_M-pyrifera_M_contig83022.19396.1|Name=mRNA_M-pyrifera_M_contig83022.19396.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=221bp
DGHNNHGHLDVMSFCLELQGQRWVVDPGHSSYNLQGYFGKQRFNFYRLNS
FGHNVVSVVGEKQGKKGTGVVESFASQSTTPEATSLGDNVASVTINATNA
YVAGGALEMKRTITAGPNWSWVDVVDTVHHPRAKRFDWRFHTFANVSTDT
NGVVFLSQDGKKIRVNATTAPSYPLIWNVTQPTFAPPDYPGDGLTAISFH
VPNPPTSTLSIRVTFAPTTV*
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mRNA from alignment at M-pyrifera_M_contig83022:35..697+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig83022.19396.1 ID=mRNA_M-pyrifera_M_contig83022.19396.1|Name=mRNA_M-pyrifera_M_contig83022.19396.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=663bp|location=Sequence derived from alignment at M-pyrifera_M_contig83022:35..697+ (Macrocystis pyrifera P11B4 male)
GATGGACACAACAATCATGGTCATCTCGATGTTATGTCCTTTTGTCTCGA GTTGCAAGGACAACGCTGGGTTGTGGACCCCGGACATTCTTCCTACAACC TACAAGGGTACTTTGGCAAGCAAAGGTTTAATTTTTACCGCCTGAATTCC TTTGGACACAATGTGGTGAGTGTTGTTGGTGAAAAGCAAGGCAAGAAAGG GACAGGTGTTGTTGAGTCGTTTGCTTCGCAGTCCACCACTCCCGAAGCCA CTTCACTAGGAGACAATGTAGCAAGTGTCACAATCAACGCTACGAATGCT TACGTTGCAGGTGGTGCTTTGGAAATGAAGAGAACCATTACTGCAGGACC GAACTGGTCATGGGTGGATGTTGTCGATACTGTGCACCATCCTCGTGCAA AGCGATTTGATTGGCGTTTCCACACGTTTGCAAATGTCTCCACTGACACA AATGGTGTTGTATTCCTTTCACAGGATGGGAAGAAGATTCGTGTCAATGC GACAACAGCTCCAAGCTATCCTCTCATCTGGAACGTGACGCAACCAACGT TTGCACCACCAGATTACCCAGGTGATGGCCTCACCGCAATCAGTTTCCAT GTACCCAACCCACCTACATCAACCCTTTCCATCCGCGTCACTTTTGCCCC CACAACGGTTTAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig83022:35..697+

>mRNA_M-pyrifera_M_contig83022.19396.1 ID=mRNA_M-pyrifera_M_contig83022.19396.1|Name=mRNA_M-pyrifera_M_contig83022.19396.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1326bp|location=Sequence derived from alignment at M-pyrifera_M_contig83022:35..697+ (Macrocystis pyrifera P11B4 male)
GATGGACACAACAATCATGGTCATCTCGATGTTATGTCCTTTTGTCTCGA
GTTGCAAGGACAACGCTGGGTTGTGGACCCCGGACATTCTTCCTACAACC
TACAAGGGTACTTTGGCAAGCAAAGGTTTAATTTTTACCGCCTGAATTCC
TTTGGACACAATGTGGTGAGTGTTGTTGGTGAAAAGCAAGGCAAGAAAGG
GACAGGTGTTGTTGAGTCGTTTGCTTCGCAGTCCACCACTCCCGAAGCCA
CTTCACTAGGAGACAATGTAGCAAGTGTCACAATCAACGCTACGAATGCT
TACGTTGCAGGTGGTGCTTTGGAAATGAAGAGAACCATTACTGCAGGACC
GAACTGGTCATGGGTGGATGTTGTCGATACTGTGCACCATCCTCGTGCAA
AGCGATTTGATTGGCGTTTCCACACGTTTGCAAATGTCTCCACTGACACA
AATGGTGTTGTATTCCTTTCACAGGATGGGAAGAAGATTCGTGTCAATGC
GACAACAGCTCCAAGCTATCCTCTCATCTGGAACGTGACGCAACCAACGT
TTGCACCACCAGATTACCCAGGTGATGGCCTCACCGCAATCAGTTTCCAT
GTACCCAACCCACCTACATCAACCCTTTCCATCCGCGTCACTTTTGCCCC
CACAACGGTTTAAGATGGACACAACAATCATGGTCATCTCGATGTTATGT
CCTTTTGTCTCGAGTTGCAAGGACAACGCTGGGTTGTGGACCCCGGACAT
TCTTCCTACAACCTACAAGGGTACTTTGGCAAGCAAAGGTTTAATTTTTA
CCGCCTGAATTCCTTTGGACACAATGTGGTGAGTGTTGTTGGTGAAAAGC
AAGGCAAGAAAGGGACAGGTGTTGTTGAGTCGTTTGCTTCGCAGTCCACC
ACTCCCGAAGCCACTTCACTAGGAGACAATGTAGCAAGTGTCACAATCAA
CGCTACGAATGCTTACGTTGCAGGTGGTGCTTTGGAAATGAAGAGAACCA
TTACTGCAGGACCGAACTGGTCATGGGTGGATGTTGTCGATACTGTGCAC
CATCCTCGTGCAAAGCGATTTGATTGGCGTTTCCACACGTTTGCAAATGT
CTCCACTGACACAAATGGTGTTGTATTCCTTTCACAGGATGGGAAGAAGA
TTCGTGTCAATGCGACAACAGCTCCAAGCTATCCTCTCATCTGGAACGTG
ACGCAACCAACGTTTGCACCACCAGATTACCCAGGTGATGGCCTCACCGC
AATCAGTTTCCATGTACCCAACCCACCTACATCAACCCTTTCCATCCGCG
TCACTTTTGCCCCCACAACGGTTTAA
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