mRNA_M-pyrifera_M_contig82943.19378.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig82943.19378.1
Unique NamemRNA_M-pyrifera_M_contig82943.19378.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Match: A0A3B1J5A0_ASTMX (Enoyl-CoA hydratase, short chain 1 n=2 Tax=Astyanax mexicanus TaxID=7994 RepID=A0A3B1J5A0_ASTMX)

HSP 1 Score: 236 bits (603), Expect = 9.440e-76
Identity = 120/159 (75.47%), Postives = 136/159 (85.53%), Query Frame = 1 
Query:    1 IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLVRAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIAAHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFVNKRKAQFKD 477
            IAAVNGFALGGGCELAMMCDII AGE A FGQPE+LLGTIPG GGTQRL RA+GKS AMEMVLTGD+  A+  +Q+GLVSKV PV   + EA++ GEKIA++SK + AMAKE VNAAFEL+LAEGNR E+RLFHSTFAT+D+KEGM AFV KRKA FKD
Sbjct:  132 IAAVNGFALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQEAKQSGLVSKVFPVDQLVSEAIKCGEKIASNSKLVAAMAKEAVNAAFELTLAEGNRLEKRLFHSTFATEDRKEGMTAFVEKRKAMFKD 290
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BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Match: J3SC55_CROAD (Enoyl-CoA hydratase, mitochondrial n=4 Tax=Serpentes TaxID=8570 RepID=J3SC55_CROAD)

HSP 1 Score: 236 bits (602), Expect = 1.340e-75
Identity = 119/159 (74.84%), Postives = 136/159 (85.53%), Query Frame = 1 
Query:    1 IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLVRAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIAAHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFVNKRKAQFKD 477
            IAAVNG+ALGGGCELAMMCDII AGE A FGQPE+LLGTIPG GGTQRL RA+GKS AMEM+LTG++  A   + AGLVSKV PV+  L EA+  GEKIA HSK ++AMAKE V+AAFELSLAEGNR E+RLFHSTFAT+D+KEGMDAFV KR+A+FKD
Sbjct:  132 IAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMILTGERISAHEAKLAGLVSKVCPVEKLLDEAISCGEKIARHSKLVVAMAKEAVDAAFELSLAEGNRLEKRLFHSTFATEDRKEGMDAFVEKREAKFKD 290
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BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Match: B5DG66_SALSA (Enoyl Coenzyme A hydratase short chain 1 mitochondrial n=23 Tax=Salmonidae TaxID=8015 RepID=B5DG66_SALSA)

HSP 1 Score: 236 bits (601), Expect = 1.840e-75
Identity = 119/160 (74.38%), Postives = 137/160 (85.62%), Query Frame = 1 
Query:    1 IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLVRAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIAAHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFVNKRKAQFKDE 480
            IAAVNGFALGGGCELAMMCDII AGE A FGQPE+LLGTIPG GGTQRL RA+GKS AMEMVLTGD+  A+  +Q+GLVSK+ PV   + EA++ GEKIAA+SK + AMAKE VNAA+EL+LAEGNR E+RLFHSTFAT+D+KEGM AFV KRKA FKD+
Sbjct:  131 IAAVNGFALGGGCELAMMCDIIFAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRITAQEAKQSGLVSKICPVDQLVSEAIKCGEKIAANSKIVSAMAKEAVNAAYELTLAEGNRLEKRLFHSTFATEDRKEGMTAFVEKRKANFKDQ 290
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BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Match: A0A8D0A863_SANLU (Enoyl-CoA hydratase, short chain 1 n=3 Tax=Perciformes TaxID=8111 RepID=A0A8D0A863_SANLU)

HSP 1 Score: 234 bits (598), Expect = 2.390e-75
Identity = 119/159 (74.84%), Postives = 136/159 (85.53%), Query Frame = 1 
Query:    1 IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLVRAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIAAHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFVNKRKAQFKD 477
            IAAVNGFALGGGCELAMMCDII AGE A FGQPE+LLGTIPG GGTQRL RA+GKS AMEMVLTGD+ +A+  +Q+GLVSKV PV   + EA++ GEKIAA+SK + AMAKE VNAAFE++LAEGN FE+RLFH+TFAT DQKEGM AFV KRKA F+D
Sbjct:  106 IAAVNGFALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRINAQDAKQSGLVSKVYPVDQLVSEAVKCGEKIAANSKLVSAMAKEAVNAAFEMTLAEGNHFEKRLFHATFATGDQKEGMTAFVEKRKASFQD 264
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BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Match: A0A8C9V6G7_SCLFO (Enoyl-CoA hydratase, short chain 1 n=3 Tax=Scleropages formosus TaxID=113540 RepID=A0A8C9V6G7_SCLFO)

HSP 1 Score: 235 bits (600), Expect = 2.690e-75
Identity = 119/160 (74.38%), Postives = 137/160 (85.62%), Query Frame = 1 
Query:    1 IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLVRAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIAAHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFVNKRKAQFKDE 480
            IAAVNG+ALGGGCELAMMCDII AGE A FGQPE+LLGTIPG GGTQRL RA+GKS AMEMVLTGD+  A+  +Q+GLVSKV P+   + EA++ GEKIAAHSK I AMAKE VNAAFEL+LAEGNR E+RLFH+TFAT+D+KEGM AFV KRKA F+D+
Sbjct:  132 IAAVNGYALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQEAKQSGLVSKVFPIDQLVPEAVKCGEKIAAHSKIISAMAKEAVNAAFELTLAEGNRLEKRLFHATFATEDRKEGMTAFVEKRKANFQDQ 291
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BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Match: A0A8C4Z1G1_GADMO (Enoyl CoA hydratase, short chain, 1, mitochondrial n=2 Tax=Gadus morhua TaxID=8049 RepID=A0A8C4Z1G1_GADMO)

HSP 1 Score: 235 bits (600), Expect = 2.770e-75
Identity = 120/159 (75.47%), Postives = 137/159 (86.16%), Query Frame = 1 
Query:    1 IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLVRAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIAAHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFVNKRKAQFKD 477
            IAAVNGFALGGGCELAMMCDII AGE A FGQPE+LLGTIPG GGTQRL RA+GKS AMEMVLTGD+ +A+  +QAGLVSKV PV   + EA++ GEKIAA+SK + AMAKE VNAA+EL+LAEGNR E+RLFHSTFAT+D+KEGM AFV KRKA F+D
Sbjct:  133 IAAVNGFALGGGCELAMMCDIIYAGEKAHFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRINAQEAKQAGLVSKVCPVDQLVPEAIKCGEKIAANSKLVSAMAKEAVNAAYELTLAEGNRLEKRLFHSTFATEDRKEGMTAFVEKRKADFQD 291
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BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Match: A0A8C7ICC5_ONCKI (Enoyl-CoA hydratase, short chain 1 n=1 Tax=Oncorhynchus kisutch TaxID=8019 RepID=A0A8C7ICC5_ONCKI)

HSP 1 Score: 236 bits (601), Expect = 3.410e-75
Identity = 119/160 (74.38%), Postives = 137/160 (85.62%), Query Frame = 1 
Query:    1 IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLVRAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIAAHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFVNKRKAQFKDE 480
            IAAVNGFALGGGCELAMMCDII AGE A FGQPE+LLGTIPG GGTQRL RA+GKS AMEMVLTGD+  A+  +Q+GLVSK+ PV   + EA++ GEKIAA+SK + AMAKE VNAA+EL+LAEGNR E+RLFHSTFAT+D+KEGM AFV KRKA FKD+
Sbjct:  151 IAAVNGFALGGGCELAMMCDIIFAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRITAQEAKQSGLVSKICPVDQLVSEAIKCGEKIAANSKIVSAMAKEAVNAAYELTLAEGNRLEKRLFHSTFATEDRKEGMTAFVEKRKANFKDQ 310
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BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Match: UPI00097CF75D (enoyl-CoA hydratase, mitochondrial n=2 Tax=Pleuronectoidei TaxID=30942 RepID=UPI00097CF75D)

HSP 1 Score: 236 bits (602), Expect = 3.680e-75
Identity = 121/159 (76.10%), Postives = 136/159 (85.53%), Query Frame = 1 
Query:    1 IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLVRAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIAAHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFVNKRKAQFKD 477
            IAAVNGFALGGGCELAMMCDII AGE A FGQPE+LLGTIPG GGTQRL RA+GKS AMEMVLTGDK +A+  +Q+GLVSKV PV   + EA++ GEKIAA+SK + AMAKE VNAAFEL+LAEGNR E+RLFHSTFAT D+KEGM AFV KRKA F+D
Sbjct:  165 IAAVNGFALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDKINAQEAKQSGLVSKVYPVDQLVSEAVKCGEKIAANSKLVSAMAKEAVNAAFELTLAEGNRLEKRLFHSTFATDDRKEGMTAFVEKRKADFQD 323
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BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Match: A0A8D0AAG4_SANLU (Enoyl-CoA hydratase, short chain 1 n=3 Tax=Sander lucioperca TaxID=283035 RepID=A0A8D0AAG4_SANLU)

HSP 1 Score: 234 bits (598), Expect = 4.920e-75
Identity = 119/159 (74.84%), Postives = 136/159 (85.53%), Query Frame = 1 
Query:    1 IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLVRAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIAAHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFVNKRKAQFKD 477
            IAAVNGFALGGGCELAMMCDII AGE A FGQPE+LLGTIPG GGTQRL RA+GKS AMEMVLTGD+ +A+  +Q+GLVSKV PV   + EA++ GEKIAA+SK + AMAKE VNAAFE++LAEGN FE+RLFH+TFAT DQKEGM AFV KRKA F+D
Sbjct:  129 IAAVNGFALGGGCELAMMCDIIYAGEKAQFGQPEILLGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRINAQDAKQSGLVSKVYPVDQLVSEAVKCGEKIAANSKLVSAMAKEAVNAAFEMTLAEGNHFEKRLFHATFATGDQKEGMTAFVEKRKASFQD 287
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BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Match: S4R7V1_PETMA (Enoyl CoA hydratase, short chain, 1, mitochondrial n=2 Tax=Petromyzon marinus TaxID=7757 RepID=S4R7V1_PETMA)

HSP 1 Score: 234 bits (596), Expect = 1.120e-74
Identity = 118/160 (73.75%), Postives = 134/160 (83.75%), Query Frame = 1 
Query:    1 IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLVRAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIAAHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFVNKRKAQFKDE 480
            IAAVNGFALGGGCELAMMCDII AGE A FGQPE+ LGTIPG GGTQRL RA+GKS AMEMVLTGD+  A+  ++AGLVSKV P    LGEA+++GEKI+ HSK +I+MAKE VNAAFELSLAEGNR E+R FH+TFAT D+KEGM AFV KRKA F D+
Sbjct:  133 IAAVNGFALGGGCELAMMCDIIYAGEKAQFGQPEIALGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQQAKEAGLVSKVCPPDQLLGEAVKLGEKISRHSKLVISMAKEAVNAAFELSLAEGNRLEKRFFHATFATDDRKEGMSAFVEKRKATFTDQ 292
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig82943.19378.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A3B1J5A0_ASTMX9.440e-7675.47Enoyl-CoA hydratase, short chain 1 n=2 Tax=Astyana... [more]
J3SC55_CROAD1.340e-7574.84Enoyl-CoA hydratase, mitochondrial n=4 Tax=Serpent... [more]
B5DG66_SALSA1.840e-7574.38Enoyl Coenzyme A hydratase short chain 1 mitochond... [more]
A0A8D0A863_SANLU2.390e-7574.84Enoyl-CoA hydratase, short chain 1 n=3 Tax=Percifo... [more]
A0A8C9V6G7_SCLFO2.690e-7574.38Enoyl-CoA hydratase, short chain 1 n=3 Tax=Sclerop... [more]
A0A8C4Z1G1_GADMO2.770e-7575.47Enoyl CoA hydratase, short chain, 1, mitochondrial... [more]
A0A8C7ICC5_ONCKI3.410e-7574.38Enoyl-CoA hydratase, short chain 1 n=1 Tax=Oncorhy... [more]
UPI00097CF75D3.680e-7576.10enoyl-CoA hydratase, mitochondrial n=2 Tax=Pleuron... [more]
A0A8D0AAG4_SANLU4.920e-7574.84Enoyl-CoA hydratase, short chain 1 n=3 Tax=Sander ... [more]
S4R7V1_PETMA1.120e-7473.75Enoyl CoA hydratase, short chain, 1, mitochondrial... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig82943contigM-pyrifera_M_contig82943:292..774 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeMetazoa
Seed ortholog score236.5
Seed ortholog evalue8.7e-60
Seed eggNOG ortholog8049.ENSGMOP00000003676
Preferred nameECHS1
KEGG rclassRC00770,RC00831,RC00834,RC01086,RC01095,RC01098,RC01103
KEGG koko:K07511
KEGG ReactionR02685,R03026,R03045,R04137,R04170,R04204,R04224,R04738,R04740,R04744,R04746,R04749,R05595,R06942
KEGG Pathwayko00062,ko00071,ko00280,ko00310,ko00380,ko00410,ko00627,ko00640,ko00650,ko00930,ko01100,ko01110,ko01120,ko01130,ko01200,ko01212,map00062,map00071,map00280,map00310,map00380,map00410,map00627,map00640,map00650,map00930,map01100,map01110,map01120,map01130,map01200,map01212
KEGG ModuleM00013,M00032,M00085,M00087
Hectar predicted targeting categoryother localisation
GOsGO:0003674,GO:0003824,GO:0004300,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005739,GO:0005759,GO:0006082,GO:0006629,GO:0006631,GO:0006635,GO:0008150,GO:0008152,GO:0009056,GO:0009062,GO:0009987,GO:0016042,GO:0016054,GO:0016829,GO:0016835,GO:0016836,GO:0019395,GO:0019752,GO:0030258,GO:0031974,GO:0032787,GO:0034440,GO:0043226,GO:0043227,GO:0043229,GO:0043231,GO:0043233,GO:0043436,GO:0044237,GO:0044238,GO:0044242,GO:0044248,GO:0044255,GO:0044281,GO:0044282,GO:0044422,GO:0044424,GO:0044429,GO:0044444,GO:0044446,GO:0044464,GO:0046395,GO:0055114,GO:0070013,GO:0071704,GO:0072329,GO:1901575
EggNOG free text desc.Enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
EggNOG OGs38F58@33154,3BC2F@33208,3D1QF@33213,487TD@7711,48X6A@7742,49W56@7898,COG1024@1,KOG1680@2759
Ec32 ortholog descriptionenoyl-CoA hydratase
Ec32 orthologEc-16_003560.1
EC4.2.1.17
COG Functional cat.I
Best tax levelActinopterygii
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko00002,ko01000
Exons1
Model size483
Cds size483
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816736.1698494-CDS-M-pyrifera_M_contig82943:291..7741622816736.1698494-CDS-M-pyrifera_M_contig82943:291..774Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82943 292..774 +
1692278147.9764388-CDS-M-pyrifera_M_contig82943:291..7741692278147.9764388-CDS-M-pyrifera_M_contig82943:291..774Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82943 292..774 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig82943.19378.1prot_M-pyrifera_M_contig82943.19378.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig82943 292..774 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig82943.19378.1

>prot_M-pyrifera_M_contig82943.19378.1 ID=prot_M-pyrifera_M_contig82943.19378.1|Name=mRNA_M-pyrifera_M_contig82943.19378.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=161bp
IAAVNGFALGGGCELAMMCDIIVAGENAIFGQPEVLLGTIPGCGGTQRLV
RAIGKSKAMEMVLTGDKWDAESMEQAGLVSKVVPVKDTLGEALQIGEKIA
AHSKPIIAMAKECVNAAFELSLAEGNRFERRLFHSTFATKDQKEGMDAFV
NKRKAQFKDE*
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mRNA from alignment at M-pyrifera_M_contig82943:292..774+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig82943.19378.1 ID=mRNA_M-pyrifera_M_contig82943.19378.1|Name=mRNA_M-pyrifera_M_contig82943.19378.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=483bp|location=Sequence derived from alignment at M-pyrifera_M_contig82943:292..774+ (Macrocystis pyrifera P11B4 male)
ATTGCTGCGGTGAATGGTTTCGCGTTGGGTGGCGGTTGCGAGCTTGCGAT
GATGTGTGACATCATCGTGGCTGGGGAGAACGCGATTTTCGGCCAGCCAG
AGGTGCTTTTGGGCACGATTCCGGGGTGTGGAGGGACGCAGCGGTTGGTT
CGTGCGATTGGCAAGTCGAAGGCGATGGAGATGGTGCTGACTGGAGACAA
GTGGGATGCGGAGAGCATGGAGCAGGCTGGGTTGGTGAGCAAAGTGGTGC
CAGTGAAGGACACTCTGGGTGAGGCGCTCCAGATTGGAGAGAAGATCGCA
GCACACTCCAAACCAATCATCGCCATGGCCAAGGAGTGCGTCAACGCCGC
ATTCGAGCTCTCTCTCGCCGAAGGAAACCGATTCGAACGCCGACTCTTCC
ACTCCACCTTCGCCACCAAAGACCAAAAGGAAGGGATGGATGCTTTCGTC
AACAAGAGGAAGGCCCAATTCAAAGACGAATGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig82943:292..774+

>mRNA_M-pyrifera_M_contig82943.19378.1 ID=mRNA_M-pyrifera_M_contig82943.19378.1|Name=mRNA_M-pyrifera_M_contig82943.19378.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=966bp|location=Sequence derived from alignment at M-pyrifera_M_contig82943:292..774+ (Macrocystis pyrifera P11B4 male)
ATTGCTGCGGTGAATGGTTTCGCGTTGGGTGGCGGTTGCGAGCTTGCGAT
GATGTGTGACATCATCGTGGCTGGGGAGAACGCGATTTTCGGCCAGCCAG
AGGTGCTTTTGGGCACGATTCCGGGGTGTGGAGGGACGCAGCGGTTGGTT
CGTGCGATTGGCAAGTCGAAGGCGATGGAGATGGTGCTGACTGGAGACAA
GTGGGATGCGGAGAGCATGGAGCAGGCTGGGTTGGTGAGCAAAGTGGTGC
CAGTGAAGGACACTCTGGGTGAGGCGCTCCAGATTGGAGAGAAGATCGCA
GCACACTCCAAACCAATCATCGCCATGGCCAAGGAGTGCGTCAACGCCGC
ATTCGAGCTCTCTCTCGCCGAAGGAAACCGATTCGAACGCCGACTCTTCC
ACTCCACCTTCGCCACCAAAGACCAAAAGGAAGGGATGGATGCTTTCGTC
AACAAGAGGAAGGCCCAATTCAAAGACGAATGAATTGCTGCGGTGAATGG
TTTCGCGTTGGGTGGCGGTTGCGAGCTTGCGATGATGTGTGACATCATCG
TGGCTGGGGAGAACGCGATTTTCGGCCAGCCAGAGGTGCTTTTGGGCACG
ATTCCGGGGTGTGGAGGGACGCAGCGGTTGGTTCGTGCGATTGGCAAGTC
GAAGGCGATGGAGATGGTGCTGACTGGAGACAAGTGGGATGCGGAGAGCA
TGGAGCAGGCTGGGTTGGTGAGCAAAGTGGTGCCAGTGAAGGACACTCTG
GGTGAGGCGCTCCAGATTGGAGAGAAGATCGCAGCACACTCCAAACCAAT
CATCGCCATGGCCAAGGAGTGCGTCAACGCCGCATTCGAGCTCTCTCTCG
CCGAAGGAAACCGATTCGAACGCCGACTCTTCCACTCCACCTTCGCCACC
AAAGACCAAAAGGAAGGGATGGATGCTTTCGTCAACAAGAGGAAGGCCCA
ATTCAAAGACGAATGA
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