mRNA_M-pyrifera_M_contig82296.19246.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig82296.19246.1
Unique NamemRNA_M-pyrifera_M_contig82296.19246.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Match: A0A7S4I3D4_9EUKA (Hypothetical protein n=1 Tax=Vannella sp. CB-2014 TaxID=1487602 RepID=A0A7S4I3D4_9EUKA)

HSP 1 Score: 164 bits (414), Expect = 1.390e-45
Identity = 73/115 (63.48%), Postives = 95/115 (82.61%), Query Frame = 1
Query:   97 EHMVVLMMENRSFDHLLGWLHEDINEIEGLDGTQWNAVNVSDPQSLKVYVDKHGYDVSPSDPNHDIDSTTEQIYGWSKPYGQPGIPNMSGFVQNTAQSGLNISNPMSMFTRAPDS 441
            EH++VLMMENRSFDH+LGWLHE   EI+GLDG++WN++N  DP S K+YV+K+G+D+SP+DPNHD++STTEQI+G+ KP  QP IP M+GFVQN+ +S LN +  MSMFT   DS
Sbjct:   28 EHVIVLMMENRSFDHMLGWLHESNAEIDGLDGSEWNSLNSQDPTSPKIYVNKNGFDISPTDPNHDLNSTTEQIFGFYKPMNQPAIPRMNGFVQNSYESNLNTTTVMSMFTEDSDS 142          
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Match: A0A6B2L347_9EUKA (Uncharacterized protein n=1 Tax=Arcella intermedia TaxID=1963864 RepID=A0A6B2L347_9EUKA)

HSP 1 Score: 104 bits (259), Expect = 2.110e-23
Identity = 54/113 (47.79%), Postives = 74/113 (65.49%), Query Frame = 1
Query:   97 EHMVVLMMENRSFDHLLGWLHEDINEIEGLDGTQWNAVNVSDPQSLKVYVDKHGYDVSPSDPNHDIDSTTEQIYGWSKPYGQPGI---PNMSGFVQNTAQSGLNISNPMSMFT 426
            ++++ +MMENRSFDHLLG+LH     I+GLDG+++N V+  DP S KV+V+++GYD  P DP H  + T E+IYG      +P I   P M GF QN    G +  NP+SMFT
Sbjct:   18 KNVITIMMENRSFDHLLGFLHLSDPAIDGLDGSEYNLVDTKDPTSEKVFVNQNGYDEGPDDPGHGWEDTAEEIYGVHDL--KPPIKLEPKMIGFAQNAKSHGHSTLNPLSMFT 128          
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Match: A0A388KDA0_CHABU (Uncharacterized protein n=1 Tax=Chara braunii TaxID=69332 RepID=A0A388KDA0_CHABU)

HSP 1 Score: 92.4 bits (228), Expect = 5.480e-19
Identity = 53/109 (48.62%), Postives = 67/109 (61.47%), Query Frame = 1
Query:   88 TLPE-----HMVVLMMENRSFDHLLGWLHEDINEIEGLDGTQWNAVNVSDPQSLKVYVDKHGYDVSPSDPNHDIDSTTEQIYG-------WSKPYGQPGIPNMSGFVQN 378
            TLP+     H+VVL+MENRSFDHLLG+L     EI+GLDGT+ N +NVSDP S +V+V  H   V P DP H   S  EQ++G       W +P  +   P M+GF QN
Sbjct:  159 TLPKDSKIKHVVVLVMENRSFDHLLGFLKRRNPEIDGLDGTESNPMNVSDPDSPRVFVADHAEYVDP-DPGHSYQSIREQVFGVPRNVTTWHRPTDEGPPPPMNGFAQN 266          
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Match: A0A0L0DSN8_THETB (Phosphatidylglycerol specific phospholipase C n=1 Tax=Thecamonas trahens ATCC 50062 TaxID=461836 RepID=A0A0L0DSN8_THETB)

HSP 1 Score: 91.3 bits (225), Expect = 1.170e-18
Identity = 48/101 (47.52%), Postives = 66/101 (65.35%), Query Frame = 1
Query:   97 EHMVVLMMENRSFDHLLGWLHEDINEIEGLDGTQWNAVNVSDPQSLKVYVDKHGYDVSPSDPNHDIDSTTEQIYGWSKPYGQPGIPN-----MSGFVQNTA 384
            +++VVLMMENRSFDH LGWL E   E++GL G+++N  N SDP + KV+V K   DV PS P H +  TT+Q++G  +    P +PN     M GFV++ A
Sbjct:   28 KNVVVLMMENRSFDHFLGWLKELNPEVDGLTGSEFNPTNTSDPSAEKVFVTKDAVDVGPS-PEHSVPGTTDQVFGHGR---IPDMPNKYPATMDGFVEDLA 124          
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Match: A0A3B6N007_WHEAT (Phospholipase C n=13 Tax=Eukaryota TaxID=2759 RepID=A0A3B6N007_WHEAT)

HSP 1 Score: 89.7 bits (221), Expect = 4.360e-18
Identity = 56/115 (48.70%), Postives = 72/115 (62.61%), Query Frame = 1
Query:  103 MVVLMMENRSFDHLLGWLHEDINEIEGLDGTQWNAVNVSDPQSLKVYV-DKHGYDVSPSDPNHDIDSTTEQIYGWSKPYGQPGIPNMSGFVQNTAQSGLNIS-NPMSMFTRAPDS 441
            +VV++MENRSFDH+LGWL     +I+GL GT+ N +N SDP S +++V DK GY    SDP H  +   EQI+G +     P  P MSGF QN    GL ++ N MS F  APDS
Sbjct:   51 VVVVVMENRSFDHVLGWLRASRPDIDGLTGTESNRLNASDPSSPEIFVTDKAGY--VDSDPGHGFEDIREQIFGSADTSAVP--PPMSGFAQNARGMGLGMAQNVMSGF--APDS 159          
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Match: A0A1S4CPT9_TOBAC (non-specific phospholipase C1-like n=10 Tax=Solanaceae TaxID=4070 RepID=A0A1S4CPT9_TOBAC)

HSP 1 Score: 89.0 bits (219), Expect = 8.130e-18
Identity = 46/98 (46.94%), Postives = 63/98 (64.29%), Query Frame = 1
Query:  103 MVVLMMENRSFDHLLGWLHEDINEIEGLDGTQWNAVNVSDPQSLKVYVDKHGYDVSPSDPNHDIDSTTEQIYGWSKPYGQPGIPNMSGFVQNTAQSGL 396
            +VVL+MENRSFDH+LGWLH+   +I+GL+G+++N VN SDP S K++V      V  SDP H I +  EQI+G +     P    M+GFVQ     G+
Sbjct:   48 LVVLVMENRSFDHILGWLHKTRPDIDGLNGSEFNLVNASDPSSPKIFVSDDAVFVD-SDPGHSIQAIREQIFGSNDSSANPAP--MNGFVQQALNMGV 142          
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Match: M1AL21_SOLTU (Hydrolase, acting on ester bonds n=5 Tax=Solanum TaxID=4107 RepID=M1AL21_SOLTU)

HSP 1 Score: 88.6 bits (218), Expect = 1.110e-17
Identity = 46/98 (46.94%), Postives = 62/98 (63.27%), Query Frame = 1
Query:  103 MVVLMMENRSFDHLLGWLHEDINEIEGLDGTQWNAVNVSDPQSLKVYVDKHGYDVSPSDPNHDIDSTTEQIYGWSKPYGQPGIPNMSGFVQNTAQSGL 396
            +VVL+MENRSFDH+LGWLH+   +I+GL G+++N VN SDP S K++V      V  SDP H I +  EQI+G +     P    M+GFVQ     G+
Sbjct:   48 LVVLVMENRSFDHILGWLHKTRPDIDGLKGSEFNRVNASDPYSSKIFVSDDAVFVD-SDPGHSIQAIREQIFGSNDSSANPAP--MNGFVQQAVNMGV 142          
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Match: UPI000E1DB36C (non-specific phospholipase C1-like isoform X1 n=1 Tax=Solanum lycopersicum TaxID=4081 RepID=UPI000E1DB36C)

HSP 1 Score: 87.8 bits (216), Expect = 1.660e-17
Identity = 45/98 (45.92%), Postives = 62/98 (63.27%), Query Frame = 1
Query:  103 MVVLMMENRSFDHLLGWLHEDINEIEGLDGTQWNAVNVSDPQSLKVYVDKHGYDVSPSDPNHDIDSTTEQIYGWSKPYGQPGIPNMSGFVQNTAQSGL 396
            +V+L+MENRSFDH+LGWLH+   +I+GL G+++N VN SDP S K++V      V  SDP H I +  EQI+G +     P    M+GFVQ     G+
Sbjct:   48 LVILVMENRSFDHILGWLHKSRPDIDGLKGSEFNRVNASDPYSSKIFVSYDAVFVD-SDPGHSIQAIGEQIFGSNDSSANPAP--MNGFVQQAVSMGV 142          
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Match: A0A1R3JMV5_COCAP (Phosphoesterase n=1 Tax=Corchorus capsularis TaxID=210143 RepID=A0A1R3JMV5_COCAP)

HSP 1 Score: 82.0 bits (201), Expect = 2.440e-17
Identity = 45/107 (42.06%), Postives = 62/107 (57.94%), Query Frame = 1
Query:  118 MENRSFDHLLGWLHEDINEIEGLDGTQWNAVNVSDPQSLKVYVDKHGYDVSPSDPNHDIDSTTEQIYGWSKPYGQPGIPNMSGFVQ---------NTAQSGLNISNP 411
            MENRSFDH+LGW+ +   EI G+DGTQWN ++ +DP S K++V+   + V P DP H   +  EQI+G +     P  P M+GF Q         N + S +N  NP
Sbjct:    1 MENRSFDHMLGWMKKINPEINGVDGTQWNPLSTTDPNSKKLFVNNQAHFVDP-DPGHSFQAIREQIFGSNDTSANP--PPMNGFAQQAYSMDPSTNMSHSVMNGFNP 104          
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Match: A0A6M2EIE5_9ROSI (Uncharacterized protein n=1 Tax=Populus davidiana TaxID=266767 RepID=A0A6M2EIE5_9ROSI)

HSP 1 Score: 86.7 bits (213), Expect = 2.670e-17
Identity = 52/121 (42.98%), Postives = 68/121 (56.20%), Query Frame = 1
Query:   13 FLFLALVLAGALAGTNAALHAEDGGTLPEHMVVLMMENRSFDHLLGWLHEDINEIEGLDGTQWNAVNVSDPQSLKVYVDKHGYDVSPSDPNHDIDSTTEQIYGWSKPYGQPGIPNMSGFVQ 375
            FLFL L L       +  +HA    T+    VV++MENRSFDH+LGW+ +   EI G+DGTQWN +N+SDP S K + +     V P DP H   +  EQI+G       P  P M+GF Q
Sbjct:   10 FLFLLLAL------LHDRIHASPIKTV----VVVVMENRSFDHMLGWMKKINPEINGVDGTQWNPLNISDPSSQKFFTNNQAQYVDP-DPGHSFQAIREQIFGSEDTSKNP--PPMNGFAQ 117          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig82296.19246.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S4I3D4_9EUKA1.390e-4563.48Hypothetical protein n=1 Tax=Vannella sp. CB-2014 ... [more]
A0A6B2L347_9EUKA2.110e-2347.79Uncharacterized protein n=1 Tax=Arcella intermedia... [more]
A0A388KDA0_CHABU5.480e-1948.62Uncharacterized protein n=1 Tax=Chara braunii TaxI... [more]
A0A0L0DSN8_THETB1.170e-1847.52Phosphatidylglycerol specific phospholipase C n=1 ... [more]
A0A3B6N007_WHEAT4.360e-1848.70Phospholipase C n=13 Tax=Eukaryota TaxID=2759 RepI... [more]
A0A1S4CPT9_TOBAC8.130e-1846.94non-specific phospholipase C1-like n=10 Tax=Solana... [more]
M1AL21_SOLTU1.110e-1746.94Hydrolase, acting on ester bonds n=5 Tax=Solanum T... [more]
UPI000E1DB36C1.660e-1745.92non-specific phospholipase C1-like isoform X1 n=1 ... [more]
A0A1R3JMV5_COCAP2.440e-1742.06Phosphoesterase n=1 Tax=Corchorus capsularis TaxID... [more]
A0A6M2EIE5_9ROSI2.670e-1742.98Uncharacterized protein n=1 Tax=Populus davidiana ... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig82296contigM-pyrifera_M_contig82296:1..441 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score94.0
Seed ortholog evalue6.4e-17
Seed eggNOG ortholog4006.Lus10036039
KEGG rclassRC00017,RC00425
KEGG koko:K01114
KEGG ReactionR01312,R02027,R02052,R03332,R07381
KEGG Pathwayko00562,ko00564,ko00565,ko01100,ko01110,ko02024,ko04919,map00562,map00564,map00565,map01100,map01110,map02024,map04919
Hectar predicted targeting categorysignal peptide
GOsGO:0003674,GO:0003824,GO:0003993,GO:0004620,GO:0004629,GO:0006629,GO:0006644,GO:0006793,GO:0006796,GO:0008081,GO:0008150,GO:0008152,GO:0009056,GO:0009395,GO:0009405,GO:0009987,GO:0016042,GO:0016298,GO:0016311,GO:0016787,GO:0016788,GO:0016791,GO:0019637,GO:0034480,GO:0035821,GO:0042578,GO:0044003,GO:0044237,GO:0044238,GO:0044242,GO:0044248,GO:0044255,GO:0044403,GO:0044419,GO:0046434,GO:0051701,GO:0051704,GO:0051817,GO:0052008,GO:0052043,GO:0052111,GO:0052185,GO:0052188,GO:0052368,GO:0071704,GO:1901575
EggNOG free text desc.Phosphoesterase family
EggNOG OGs2QPJ0@2759,37S0T@33090,3GBCP@35493,4JIYS@91835,COG3511@1
EC3.1.4.3
COG Functional cat.M
Best tax levelfabids
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko02042
Exons1
Model size441
Cds size441
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816729.8708172-CDS-M-pyrifera_M_contig82296:0..4411622816729.8708172-CDS-M-pyrifera_M_contig82296:0..441Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82296 1..441 -
1692278144.6733322-CDS-M-pyrifera_M_contig82296:0..4411692278144.6733322-CDS-M-pyrifera_M_contig82296:0..441Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig82296 1..441 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig82296.19246.1prot_M-pyrifera_M_contig82296.19246.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig82296 1..441 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig82296.19246.1

>prot_M-pyrifera_M_contig82296.19246.1 ID=prot_M-pyrifera_M_contig82296.19246.1|Name=mRNA_M-pyrifera_M_contig82296.19246.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=147bp
MAHQFLFLALVLAGALAGTNAALHAEDGGTLPEHMVVLMMENRSFDHLLG
WLHEDINEIEGLDGTQWNAVNVSDPQSLKVYVDKHGYDVSPSDPNHDIDS
TTEQIYGWSKPYGQPGIPNMSGFVQNTAQSGLNISNPMSMFTRAPDS
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mRNA from alignment at M-pyrifera_M_contig82296:1..441-

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig82296.19246.1 ID=mRNA_M-pyrifera_M_contig82296.19246.1|Name=mRNA_M-pyrifera_M_contig82296.19246.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=441bp|location=Sequence derived from alignment at M-pyrifera_M_contig82296:1..441- (Macrocystis pyrifera P11B4 male)
ATGGCGCACCAATTCCTGTTTTTGGCTTTGGTTCTGGCAGGGGCTCTGGC CGGGACCAACGCAGCATTGCACGCCGAAGACGGAGGCACCCTGCCTGAGC ACATGGTAGTGCTGATGATGGAGAACCGGTCTTTTGACCATCTGCTGGGC TGGCTTCATGAAGACATCAACGAAATCGAGGGCCTCGACGGAACTCAGTG GAATGCTGTCAATGTTTCCGATCCGCAGTCGCTGAAAGTGTACGTCGACA AGCATGGATACGACGTCTCCCCTTCCGACCCCAACCATGACATAGACTCA ACCACAGAGCAAATCTATGGTTGGTCAAAGCCTTACGGTCAGCCTGGGAT CCCGAACATGAGCGGTTTCGTGCAGAACACTGCTCAGTCGGGCCTCAACA TCAGCAACCCCATGAGCATGTTTACCAGGGCTCCCGACAGT
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig82296:1..441-

>mRNA_M-pyrifera_M_contig82296.19246.1 ID=mRNA_M-pyrifera_M_contig82296.19246.1|Name=mRNA_M-pyrifera_M_contig82296.19246.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=882bp|location=Sequence derived from alignment at M-pyrifera_M_contig82296:1..441- (Macrocystis pyrifera P11B4 male)
ATGGCGCACCAATTCCTGTTTTTGGCTTTGGTTCTGGCAGGGGCTCTGGC
CGGGACCAACGCAGCATTGCACGCCGAAGACGGAGGCACCCTGCCTGAGC
ACATGGTAGTGCTGATGATGGAGAACCGGTCTTTTGACCATCTGCTGGGC
TGGCTTCATGAAGACATCAACGAAATCGAGGGCCTCGACGGAACTCAGTG
GAATGCTGTCAATGTTTCCGATCCGCAGTCGCTGAAAGTGTACGTCGACA
AGCATGGATACGACGTCTCCCCTTCCGACCCCAACCATGACATAGACTCA
ACCACAGAGCAAATCTATGGTTGGTCAAAGCCTTACGGTCAGCCTGGGAT
CCCGAACATGAGCGGTTTCGTGCAGAACACTGCTCAGTCGGGCCTCAACA
TCAGCAACCCCATGAGCATGTTTACCAGGGCTCCCGACAGTATGGCGCAC
CAATTCCTGTTTTTGGCTTTGGTTCTGGCAGGGGCTCTGGCCGGGACCAA
CGCAGCATTGCACGCCGAAGACGGAGGCACCCTGCCTGAGCACATGGTAG
TGCTGATGATGGAGAACCGGTCTTTTGACCATCTGCTGGGCTGGCTTCAT
GAAGACATCAACGAAATCGAGGGCCTCGACGGAACTCAGTGGAATGCTGT
CAATGTTTCCGATCCGCAGTCGCTGAAAGTGTACGTCGACAAGCATGGAT
ACGACGTCTCCCCTTCCGACCCCAACCATGACATAGACTCAACCACAGAG
CAAATCTATGGTTGGTCAAAGCCTTACGGTCAGCCTGGGATCCCGAACAT
GAGCGGTTTCGTGCAGAACACTGCTCAGTCGGGCCTCAACATCAGCAACC
CCATGAGCATGTTTACCAGGGCTCCCGACAGT
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