mRNA_M-pyrifera_M_contig81807.19148.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig81807.19148.1
Unique NamemRNA_M-pyrifera_M_contig81807.19148.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Match: A0A661D5B6_9GAMM (Nitroreductase (Fragment) n=1 Tax=Gammaproteobacteria bacterium TaxID=1913989 RepID=A0A661D5B6_9GAMM)

HSP 1 Score: 157 bits (398), Expect = 2.530e-47
Identity = 73/126 (57.94%), Postives = 89/126 (70.63%), Query Frame = 1
Query:    1 HMRLFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVVERDPSAYRRLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMYHFTVGVAAEDARLRTLPPYAHLEGR 378
            H++ ++L  G+ + +A+  SC Q+IAAD AFSLGML      +   P  YRRLFWE G IGQ LYLEAEA G   TGIGCYFDD+VH+L+GL    +Q MYHFTVG    D RL+TLPPYAHLEG+
Sbjct:    8 HLQFYQLVSGNAQTIAQQMSCHQEIAADSAFSLGMLAEFETGLAVGPFGYRRLFWEAGIIGQVLYLEAEAVGVRGTGIGCYFDDAVHSLLGLADTHYQSMYHFTVGTPQHDKRLQTLPPYAHLEGK 133          
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Match: UPI0004B2A726 (SagB/ThcOx family dehydrogenase n=1 Tax=Methylomicrobium lacus TaxID=136992 RepID=UPI0004B2A726)

HSP 1 Score: 164 bits (416), Expect = 8.420e-46
Identity = 76/123 (61.79%), Postives = 92/123 (74.80%), Query Frame = 1
Query:   10 LFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVVERDPSAYRRLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMYHFTVGVAAEDARLRTLPPYAHLEGR 378
            ++RL  G+C+ +A++ SC QDIA DGAFSL ML      + R+P  YRRLFWECG IGQALYLEAEAAG   TGIGC+FDDSVH ++GL  + +Q +YHFTVG A EDARL+TLP Y HL  R
Sbjct:  416 VYRLVAGNCQRMAKTVSCHQDIAGDGAFSLAMLAEFQQPISREPWCYRRLFWECGLIGQALYLEAEAAGMRGTGIGCFFDDSVHDILGLKDNRFQSLYHFTVGQALEDARLQTLPAYHHLSQR 538          
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Match: A0A3C1VDW2_9BACT (Nitroreductase n=1 Tax=Verrucomicrobia subdivision 3 bacterium TaxID=2052186 RepID=A0A3C1VDW2_9BACT)

HSP 1 Score: 164 bits (416), Expect = 8.540e-46
Identity = 79/123 (64.23%), Postives = 90/123 (73.17%), Query Frame = 1
Query:   10 LFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVV-ERDPSAYRRLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMYHFTVGVAAEDARLRTLPPYAHLEG 375
            L+RL KG     AR+ SC QDIAADGAFSLGML R  PV+ +R  +AYR LFWE G IGQALYL AEAAG   TGIGCYFDD +H  +GL G+ WQ +YHFT+G   ED RLRT+PPYAH  G
Sbjct:  415 LYRLGKGDLRDFARTLSCHQDIAADGAFSLGMLARFEPVLRDRGAAAYRELFWEAGMIGQALYLAAEAAGVRGTGIGCYFDDVLHRALGLAGNDWQSLYHFTIGGPVEDLRLRTVPPYAHRAG 537          
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Match: A0A496V2S4_9GAMM (Uncharacterized protein n=2 Tax=Gammaproteobacteria TaxID=1236 RepID=A0A496V2S4_9GAMM)

HSP 1 Score: 156 bits (394), Expect = 1.440e-45
Identity = 74/124 (59.68%), Postives = 87/124 (70.16%), Query Frame = 1
Query:    1 HMRLFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVVERDPSAYRRLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMYHFTVGVAAEDARLRTLPPYAHLE 372
            H+  ++L  G  + V +  SC Q+IAAD AFSLGML      ++  P  YRRLFWE G IGQ LYLEAEA G   TGIGCYFDD+VHAL+GLTG  +Q MYHFTVG    D RL+TLPPYAHL+
Sbjct:   96 HLPFYQLVSGDAQTVVQQISCHQEIAADSAFSLGMLAEFEESLKNRPWGYRRLFWEAGIIGQVLYLEAEAVGVRGTGIGCYFDDAVHALLGLTGTCYQSMYHFTVGSPLIDKRLQTLPPYAHLD 219          
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Match: A0A257NFL8_9GAMM (Nitroreductase (Fragment) n=1 Tax=Methylococcaceae bacterium NSO1 TaxID=1917483 RepID=A0A257NFL8_9GAMM)

HSP 1 Score: 155 bits (393), Expect = 2.560e-45
Identity = 71/121 (58.68%), Postives = 84/121 (69.42%), Query Frame = 1
Query:   10 LFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVVERDPSAYRRLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMYHFTVGVAAEDARLRTLPPYAHLE 372
            L+ L  G C+ +A++ SC Q IA+D AF+LGM+      +E  P  YRRLFWECG IGQ LYLEAEAAG   TGIGCYFDDSVH L+G   D +Q +YHFTVG   ED RL TLP Y HL+
Sbjct:  104 LYHLSSGECKQIAKTVSCHQPIASDSAFTLGMVAEFSETIETAPWLYRRLFWECGLIGQVLYLEAEAAGIRGTGIGCYFDDSVHELLGFKDDKFQSLYHFTVGKPLEDKRLETLPAYGHLK 224          
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Match: G2E8A0_9GAMM (Nitroreductase (Fragment) n=1 Tax=Thiorhodococcus drewsii AZ1 TaxID=765913 RepID=G2E8A0_9GAMM)

HSP 1 Score: 159 bits (403), Expect = 3.040e-45
Identity = 74/127 (58.27%), Postives = 88/127 (69.29%), Query Frame = 1
Query:    1 HMRLFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVVERDPSAYRRLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMYHFTVGVAAEDARLRTLPPYAHLEGRS 381
            H+ L+RL +G     AR+ SC QDIA+DG F++ ML      +E  P  YR LFWECG IGQ LYLEAEAAG   TGIGC+FDD+VH L+G+    WQ +YHFTVG   ED RLRT PPYAHL+ RS
Sbjct:  243 HLGLYRLVEGDARNAARALSCHQDIASDGCFAVAMLADFDAALEHGPWRYRELFWECGLIGQILYLEAEAAGLRGTGIGCFFDDAVHDLLGIQDRRWQSLYHFTVGGPVEDPRLRTEPPYAHLDERS 369          
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Match: UPI001922141E (SagB/ThcOx family dehydrogenase n=1 Tax=Methylomicrobium sp. RS1 TaxID=2802053 RepID=UPI001922141E)

HSP 1 Score: 163 bits (412), Expect = 3.070e-45
Identity = 73/121 (60.33%), Postives = 91/121 (75.21%), Query Frame = 1
Query:   10 LFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVVERDPSAYRRLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMYHFTVGVAAEDARLRTLPPYAHLE 372
            L+RL  G+    A++ SC Q IA+D AFSLGML +    ++ +P  YRRLFWECG +GQ LYLEAEAAG   TGIGC+FDD+VH ++GLT D WQ +YHFTVG A EDARL++LPPY HL+
Sbjct:  415 LYRLFAGNVRQAAKTLSCHQSIASDSAFSLGMLAQFADNIDAEPWRYRRLFWECGLLGQILYLEAEAAGVRGTGIGCFFDDAVHGVLGLTDDRWQSLYHFTVGEALEDARLQSLPPYGHLQ 535          
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Match: UPI001E40236C (nitroreductase family protein n=1 Tax=Acidihalobacter yilgarnensis TaxID=2819280 RepID=UPI001E40236C)

HSP 1 Score: 153 bits (386), Expect = 6.040e-44
Identity = 71/123 (57.72%), Postives = 88/123 (71.54%), Query Frame = 1
Query:    1 HMRLFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVVERDPSAYRRLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMYHFTVGVAAEDARLRTLPPYAHL 369
            H+ L+R+++G     AR+ SC QDIAADGAFSL ML+     + + P  YR+LFWE G +GQ LYLEAEAAG   TGIGC+FDD VH L+G+ G   Q +YHFT+G A  DARL+TLP YAHL
Sbjct:  125 HLALYRMQEGDARAAARTLSCHQDIAADGAFSLAMLSEFERDLTQAPWCYRQLFWEAGMLGQTLYLEAEAAGLRGTGIGCFFDDPVHELIGIEGTALQSLYHFTIGGAHVDARLQTLPAYAHL 247          
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Match: G3IZ92_METTV (Nitroreductase n=4 Tax=Methylobacter tundripaludum TaxID=173365 RepID=G3IZ92_METTV)

HSP 1 Score: 159 bits (402), Expect = 9.810e-44
Identity = 73/121 (60.33%), Postives = 84/121 (69.42%), Query Frame = 1
Query:   10 LFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVVERDPSAYRRLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMYHFTVGVAAEDARLRTLPPYAHLE 372
            L+ L  G C  +A++ SC Q IA+D AFSLGM+   G  +E  P  YRRLFWECG IGQ LYLEAEAAG   TGIGCYFDDSVH L+G   D +Q +YHFTVG   ED RL TLP Y HL+
Sbjct:  421 LYHLHSGDCRQIAKTLSCHQPIASDSAFSLGMVAEFGETIEAAPWLYRRLFWECGLIGQTLYLEAEAAGLRGTGIGCYFDDSVHELLGFKDDKFQSLYHFTVGKPLEDKRLETLPAYGHLD 541          
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Match: A0A0F7KFS7_9PROT (Nitroreductase n=4 Tax=Nitrosomonas TaxID=914 RepID=A0A0F7KFS7_9PROT)

HSP 1 Score: 159 bits (401), Expect = 1.330e-43
Identity = 76/126 (60.32%), Postives = 87/126 (69.05%), Query Frame = 1
Query:    1 HMRLFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVVERDPSAYRRLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMYHFTVGVAAEDARLRTLPPYAHLEGR 378
            H+ LFRL  G     AR  SC QDIAAD  FSLGML      +      YR LF E G IGQ LYLEAEAAG   TGIGCYFDD++H+++GL+G  WQ +YHFTVG A +D+RLRTLPPYAHL GR
Sbjct:  414 HLGLFRLFAGDVRDTARLISCHQDIAADSCFSLGMLAEFETALNEGAWHYRELFHEAGMIGQVLYLEAEAAGLRGTGIGCYFDDALHSMLGLSGHAWQSLYHFTVGGALDDSRLRTLPPYAHLSGR 539          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig81807.19148.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A661D5B6_9GAMM2.530e-4757.94Nitroreductase (Fragment) n=1 Tax=Gammaproteobacte... [more]
UPI0004B2A7268.420e-4661.79SagB/ThcOx family dehydrogenase n=1 Tax=Methylomic... [more]
A0A3C1VDW2_9BACT8.540e-4664.23Nitroreductase n=1 Tax=Verrucomicrobia subdivision... [more]
A0A496V2S4_9GAMM1.440e-4559.68Uncharacterized protein n=2 Tax=Gammaproteobacteri... [more]
A0A257NFL8_9GAMM2.560e-4558.68Nitroreductase (Fragment) n=1 Tax=Methylococcaceae... [more]
G2E8A0_9GAMM3.040e-4558.27Nitroreductase (Fragment) n=1 Tax=Thiorhodococcus ... [more]
UPI001922141E3.070e-4560.33SagB/ThcOx family dehydrogenase n=1 Tax=Methylomic... [more]
UPI001E40236C6.040e-4457.72nitroreductase family protein n=1 Tax=Acidihalobac... [more]
G3IZ92_METTV9.810e-4460.33Nitroreductase n=4 Tax=Methylobacter tundripaludum... [more]
A0A0F7KFS7_9PROT1.330e-4360.32Nitroreductase n=4 Tax=Nitrosomonas TaxID=914 RepI... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig81807contigM-pyrifera_M_contig81807:531..917 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score159.1
Seed ortholog evalue1.4e-36
Seed eggNOG ortholog697282.Mettu_3395
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Nitroreductase family
EggNOG OGs1Q0G9@1224,1RS1G@1236,1XEMQ@135618,COG0778@1,COG0778@2
COG Functional cat.C
Best tax levelMethylococcales
Best eggNOG OGNA|NA|NA
Exons1
Model size387
Cds size384
Stop1
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816725.61985-CDS-M-pyrifera_M_contig81807:530..9141622816725.61985-CDS-M-pyrifera_M_contig81807:530..914Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81807 531..914 -
1692278142.5638697-CDS-M-pyrifera_M_contig81807:530..9141692278142.5638697-CDS-M-pyrifera_M_contig81807:530..914Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig81807 531..914 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816725.6308854-UTR-M-pyrifera_M_contig81807:914..9171622816725.6308854-UTR-M-pyrifera_M_contig81807:914..917Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig81807 915..917 -
1692278142.578745-UTR-M-pyrifera_M_contig81807:914..9171692278142.578745-UTR-M-pyrifera_M_contig81807:914..917Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig81807 915..917 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig81807.19148.1prot_M-pyrifera_M_contig81807.19148.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig81807 531..914 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig81807.19148.1

>prot_M-pyrifera_M_contig81807.19148.1 ID=prot_M-pyrifera_M_contig81807.19148.1|Name=mRNA_M-pyrifera_M_contig81807.19148.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=128bp
MRLFRLRKGHCEGVARSASCQQDIAADGAFSLGMLTRLGPVVERDPSAYR
RLFWECGAIGQALYLEAEAAGQNATGIGCYFDDSVHALVGLTGDTWQVMY
HFTVGVAAEDARLRTLPPYAHLEGRSE*
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mRNA from alignment at M-pyrifera_M_contig81807:531..917-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig81807.19148.1 ID=mRNA_M-pyrifera_M_contig81807.19148.1|Name=mRNA_M-pyrifera_M_contig81807.19148.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=387bp|location=Sequence derived from alignment at M-pyrifera_M_contig81807:531..917- (Macrocystis pyrifera P11B4 male)
CACATGCGCCTCTTCCGCCTCCGCAAGGGCCACTGCGAAGGCGTCGCGCG CAGCGCCTCCTGCCAACAGGACATCGCCGCCGACGGCGCCTTCAGCCTGG GAATGCTGACGCGCCTCGGGCCGGTCGTCGAGCGCGACCCCAGCGCGTAC CGCCGCCTGTTCTGGGAGTGCGGCGCGATCGGCCAAGCGCTCTACCTCGA GGCCGAGGCCGCCGGCCAGAACGCAACCGGCATCGGGTGCTACTTCGACG ACTCGGTGCACGCGCTCGTCGGCCTGACCGGCGACACGTGGCAGGTGATG TACCACTTCACGGTCGGCGTCGCGGCCGAGGACGCGCGCCTGCGGACGCT GCCGCCGTACGCCCACCTCGAGGGCCGCAGCGAGTGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig81807:531..917-

>mRNA_M-pyrifera_M_contig81807.19148.1 ID=mRNA_M-pyrifera_M_contig81807.19148.1|Name=mRNA_M-pyrifera_M_contig81807.19148.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=768bp|location=Sequence derived from alignment at M-pyrifera_M_contig81807:531..917- (Macrocystis pyrifera P11B4 male)
ATGCGCCTCTTCCGCCTCCGCAAGGGCCACTGCGAAGGCGTCGCGCGCAG
CGCCTCCTGCCAACAGGACATCGCCGCCGACGGCGCCTTCAGCCTGGGAA
TGCTGACGCGCCTCGGGCCGGTCGTCGAGCGCGACCCCAGCGCGTACCGC
CGCCTGTTCTGGGAGTGCGGCGCGATCGGCCAAGCGCTCTACCTCGAGGC
CGAGGCCGCCGGCCAGAACGCAACCGGCATCGGGTGCTACTTCGACGACT
CGGTGCACGCGCTCGTCGGCCTGACCGGCGACACGTGGCAGGTGATGTAC
CACTTCACGGTCGGCGTCGCGGCCGAGGACGCGCGCCTGCGGACGCTGCC
GCCGTACGCCCACCTCGAGGGCCGCAGCGAGTGAATGCGCCTCTTCCGCC
TCCGCAAGGGCCACTGCGAAGGCGTCGCGCGCAGCGCCTCCTGCCAACAG
GACATCGCCGCCGACGGCGCCTTCAGCCTGGGAATGCTGACGCGCCTCGG
GCCGGTCGTCGAGCGCGACCCCAGCGCGTACCGCCGCCTGTTCTGGGAGT
GCGGCGCGATCGGCCAAGCGCTCTACCTCGAGGCCGAGGCCGCCGGCCAG
AACGCAACCGGCATCGGGTGCTACTTCGACGACTCGGTGCACGCGCTCGT
CGGCCTGACCGGCGACACGTGGCAGGTGATGTACCACTTCACGGTCGGCG
TCGCGGCCGAGGACGCGCGCCTGCGGACGCTGCCGCCGTACGCCCACCTC
GAGGGCCGCAGCGAGTGA
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