mRNA_M-pyrifera_M_contig79330.18626.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig79330.18626.1
Unique NamemRNA_M-pyrifera_M_contig79330.18626.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Match: A0A7S2S9H7_9STRA (Hypothetical protein n=1 Tax=labyrinthulid quahog parasite QPX TaxID=96639 RepID=A0A7S2S9H7_9STRA)

HSP 1 Score: 82.8 bits (203), Expect = 5.060e-15
Identity = 53/127 (41.73%), Postives = 68/127 (53.54%), Query Frame = 1
Query:  160 QWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGIQVATALLVLIAALVSHATYHPYHTPLLNH-LDTISLVTSCITLLGGLFIVLDDAGEAGLATGIAAPTASVFIVLANSLFFFY 537
            +WR   GF+ +GYR   + WE VV LRK AFA V+  L P G  IQ    +LVL+A++  H  YHPY      H L+T SL    +TL+ GLFI   +               ++FIVLAN LFF Y
Sbjct:  311 KWRPVYGFIYSGYRKECYFWEIVVMLRKAAFATVSVVLRPVGVDIQTYVGMLVLVASIALHIHYHPYSAVSRLHVLETTSLSICFLTLVCGLFIYSPN------TMTTFKQGCTMFIVLANVLFFLY 431          
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Match: A0A078B8W1_STYLE (Uncharacterized protein n=1 Tax=Stylonychia lemnae TaxID=5949 RepID=A0A078B8W1_STYLE)

HSP 1 Score: 81.6 bits (200), Expect = 1.420e-14
Identity = 56/199 (28.14%), Postives = 100/199 (50.25%), Query Frame = 1
Query:    1 RLAMNLDTRCTDPDNLALMLSMSLPIIILVRIGFPVASWVFLRSIGPRKLQTLQWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGIQVATALLVLIAALVSHATYHPYHTPLLNHLDTISLVTSCITLLGGLFIVLDDAGEAGLATGIAAPTASVFIVLANSLFFFY-----GAGLFIYYVYPLISK 582
            R+  +L+  C    +    L ++LP +I+  +G P+ ++ FL       +++L+ R + GFL  GY+   F WE V+  RK+   F+   L  +G   Q    LL+LI  +  + T +P+ T  LN L+T+SL+TS +T+  G+F ++       + TG    ++ V +     L  F+         FIY+VY + ++
Sbjct:  602 RIKTDLEILCYQGQHSMWALGVALPALIVWGLGIPMFAF-FLLQKDKLTIESLETRQKYGFLYRGYKRRFFYWEIVITYRKIFLIFIQVFLVQAGVITQAMLVLLMLILFMGVNLTKNPFQTVALNELETLSLITSLLTIFCGIFFIV-SVDTTDVETGNENSSSQVSLNENTKLMLFFIILFSNMAFFIYWVYKMFNE 798          
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Match: A0A078ADQ5_STYLE (Uncharacterized protein n=1 Tax=Stylonychia lemnae TaxID=5949 RepID=A0A078ADQ5_STYLE)

HSP 1 Score: 81.3 bits (199), Expect = 2.090e-14
Identity = 58/193 (30.05%), Postives = 99/193 (51.30%), Query Frame = 1
Query:    1 RLAMNLDTRCTDPDNLALMLSMSLPIIILVRIGFPVASWVFLRSIGPRKLQTLQWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGIQVATALLVLIAALVSHATYHPYHTPLLNHLDTISLVTSCITLLGGLFIVLDDAGE----------AGLATGIAAPTASVF----IVLANSLFFFY 537
            R+  +L T C  P +      +++P I++  +G P  ++V L     +KL TL+ R +LGFL NGY+   + WE V+  RK+   F+   +   G   Q     L+LI  L+ +    P+   +LN L+T+SL+TS +++  G++ V +   E          AG++ G    + S+F    I+++N LFF Y
Sbjct: 4049 RIYKDLQTMCWSPIHTFWSYFVAMPSIVVWGLGIPAFAFVLLTR-DRQKLNTLEIREKLGFLYNGYKHDFYYWEIVIMYRKIILIFIAVFIQNYGVMTQALIVFLLLIICLILNIKTKPFFLVVLNDLETLSLITSILSVYCGIYFVSNIPQEFQEDIPQEVKAGISLG---KSTSLFFFAVILISNLLFFLY 4237          
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Match: J9IX39_9SPIT (Uncharacterized protein n=2 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9IX39_9SPIT)

HSP 1 Score: 80.5 bits (197), Expect = 3.840e-14
Identity = 53/178 (29.78%), Postives = 92/178 (51.69%), Query Frame = 1
Query:    1 RLAMNLDTRCTDPDNLALMLSMSLPIIILVRIGFPVASWVFLRSIGPRKLQTLQWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGIQVATALLVLIAALVSHATYHPYHTPLLNHLDTISLVTSCITLLGGLFIVLDDAGEAGLATGIAAPTASVFIVLANSLFFF 534
            R+  +L+  C +  +    L+++LP +I+  +G P+ +++ L     R L  +  R + GFL  GY+   + WE V+  RK+   F+   L   G   Q    LL+LIA +  + T +P+ T  LN L+T+SL+TS +T+  G+F ++       L TG  + ++ V +     LF F
Sbjct: 3492 RVKNDLEISCYEGQHSLWALAIALPALIVWGLGIPMFAFILLTRESKR-LDQIATRQKYGFLFRGYKQRFYYWEIVIMYRKIFLIFIQVFLVNYGVMTQAMLVLLMLIAFMSINLTKYPFQTISLNQLETLSLITSLLTIFCGVFYIV-SVDSTNLDTGETSSSSEVVLNEDTKLFLF 3667          
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Match: A0A078AZK6_STYLE (Uncharacterized protein n=1 Tax=Stylonychia lemnae TaxID=5949 RepID=A0A078AZK6_STYLE)

HSP 1 Score: 79.7 bits (195), Expect = 6.150e-14
Identity = 52/146 (35.62%), Postives = 79/146 (54.11%), Query Frame = 1
Query:   13 NLDTRCTDPDNLALMLSMSLPIIILVRIGFPVASWVFLRSIGPRKLQTLQWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGI-QVATALLVLIAALVSHATYHPYHTPLLNHLDTISLVTSCITLLGGLFIVLD 447
            +L+  C D  +      ++LP I++  IG P+ + + L  I    L  +  R   GFL  GY+   + WE V+  RK+   F+T  +  S G I Q    L+VLI  LV +  + P++T  LN L+TISLVTS I++  G+F +LD
Sbjct:  142 DLEILCWDKSHQFYSYFVALPSILVWGIGLPILALIILIKIR-NNLNRVSIREMYGFLYRGYKVQFYYWEIVITSRKILIIFITVFVGTSFGLIAQALIMLIVLILFLVINHQFKPFNTQALNDLETISLVTSMISVYSGIFFLLD 286          
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Match: A0A078A232_STYLE (Uncharacterized protein n=1 Tax=Stylonychia lemnae TaxID=5949 RepID=A0A078A232_STYLE)

HSP 1 Score: 79.3 bits (194), Expect = 9.150e-14
Identity = 58/191 (30.37%), Postives = 94/191 (49.21%), Query Frame = 1
Query:    1 RLAMNLDTRCTDPDNLALMLSMSLPIIILVRIGFPVASWVFLRSIGPRKLQTLQWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGIQVATALLVLIAALVSHATYHPYHTPLLNHLDTISLVTSCITLLGGLFIVLDDA------------GEAGLATGIAAPTASVFIVLANSLFFFY 537
            R+  +L+  C  P++      ++LP II+  +G P  +   L     +KL + + R R GFL  GYR   + WE V+  RK+   F    ++  G   Q      +LI  L+ +    P++T +LN L+T+SL+TS IT+  GLF +L+              G   L+TG      ++ I+++N LFF Y
Sbjct:  814 RVQDDLEIICWSPEHTFYSYFVALPCIIVWGLGIPFFALAILMR-KRKKLDSFEIRQRFGFLYRGYRKDYYYWEIVIMYRKILIIFTAVFISNFGIVAQALIVFFILIIFLMINFKKQPFNTLILNDLETLSLITSMITIYCGLFFILNKPKEWIEENPDYARGSVSLSTGFQRLFFAL-ILVSNLLFFTY 1002          
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Match: J9IMW4_9SPIT (Uncharacterized protein n=1 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9IMW4_9SPIT)

HSP 1 Score: 79.0 bits (193), Expect = 1.260e-13
Identity = 46/149 (30.87%), Postives = 78/149 (52.35%), Query Frame = 1
Query:    1 RLAMNLDTRCTDPDNLALMLSMSLPIIILVRIGFPVASWVFLRSIGPRKLQTLQWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGIQVATALLVLIAALVSHATYHPYHTPLLNHLDTISLVTSCITLLGGLFIVLD 447
            R+  +L+  C D  +      ++LP I L  +G P  +++ +  +    L  ++ R + GFL  GYR   + WE V+  RK+   F+   +   G   Q     +VLIA L+++    P+ T  LN L+T+SL+TS IT+  G+F V++
Sbjct: 1168 RVLNDLEVLCWDTQHKFFSYFVALPSIFLWGLGIPFFAFILMLKV-KNNLDLIEVREKFGFLYRGYRNDFYYWEIVIMYRKILIIFIAVFVKNFGVIAQALVVFIVLIAFLMTNTEKQPFSTKALNDLETLSLITSMITIYCGIFFVIN 1315          
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Match: J9F519_9SPIT (Uncharacterized protein n=1 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9F519_9SPIT)

HSP 1 Score: 79.0 bits (193), Expect = 1.290e-13
Identity = 52/178 (29.21%), Postives = 91/178 (51.12%), Query Frame = 1
Query:    1 RLAMNLDTRCTDPDNLALMLSMSLPIIILVRIGFPVASWVFLRSIGPRKLQTLQWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGIQVATALLVLIAALVSHATYHPYHTPLLNHLDTISLVTSCITLLGGLFIVLDDAGEAGLATGIAAPTASVFIVLANSLFFF 534
            R+  +L+  C +  +    L+++LP +I+  +G P+ +++ L     R L  +  R + GFL  GY+   + WE V+  RK+   F+   L   G   Q    LL+LI  +  + T +P+ T  LN L+T+SL+TS +T+  G+F ++       L TG  + ++ V +     LF F
Sbjct: 2268 RVKNDLEISCYEGQHSLWALAIALPALIVWGLGIPMFAFILLTRESKR-LDQIATRQKYGFLFRGYKQRFYYWEIVIMYRKIFLIFIQVFLVNYGVMTQAMLVLLMLITFMSINLTKYPFQTISLNQLETLSLITSLLTIFCGVFYIV-SVDSTNLDTGETSSSSEVVLNEDTKLFLF 2443          
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Match: J9IYZ4_9SPIT (Uncharacterized protein n=1 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9IYZ4_9SPIT)

HSP 1 Score: 79.0 bits (193), Expect = 1.310e-13
Identity = 46/149 (30.87%), Postives = 78/149 (52.35%), Query Frame = 1
Query:    1 RLAMNLDTRCTDPDNLALMLSMSLPIIILVRIGFPVASWVFLRSIGPRKLQTLQWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGIQVATALLVLIAALVSHATYHPYHTPLLNHLDTISLVTSCITLLGGLFIVLD 447
            R+  +L+  C D  +      ++LP I L  +G P  +++ +  +    L  ++ R + GFL  GYR   + WE V+  RK+   F+   +   G   Q     +VLIA L+++    P+ T  LN L+T+SL+TS IT+  G+F V++
Sbjct: 3713 RVLNDLEVLCWDTQHKFFSYFVALPSIFLWGLGIPFFAFILMLKV-KNNLDLIEVREKFGFLYRGYRNDFYYWEIVIMYRKILIIFIAVFVKNFGVIAQALVVFIVLIAFLMTNTEKQPFSTKALNDLETLSLITSMITIYCGIFFVIN 3860          
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Match: J9HYM5_9SPIT (Uncharacterized protein n=1 Tax=Oxytricha trifallax TaxID=1172189 RepID=J9HYM5_9SPIT)

HSP 1 Score: 79.0 bits (193), Expect = 1.310e-13
Identity = 47/153 (30.72%), Postives = 83/153 (54.25%), Query Frame = 1
Query:    1 RLAMNLDTRCTDPDNLALMLSMSLPIIILVRIGFPVASWVFLRSIGPRKLQTLQWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGIQVATALLVLIAALVSHATYHPYHTPLLNHLDTISLVTSCITLLGGLFIVLDDAGE 459
            R+  +L+  C + ++      ++LP I +  +G P+ +++ L  +    L ++Q R + GFL  GYR   + WES++  RK+   F++  ++  G   Q     +VLI  L+ ++   P+ T  LN L+T+SLVTS IT+  GLF + +   E
Sbjct: 4231 RIQDDLEVVCWNAEHKLYSYFIALPSIFVWGLGIPLFAFILLSKVR-FNLDSIQVREKYGFLYRGYRKDFYFWESIIMYRKIMLIFISVFVSNLGVIAQALVVFMVLICFLIVNSKKKPFSTIALNDLETLSLVTSMITVYCGLFFISNKPEE 4382          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig79330.18626.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A7S2S9H7_9STRA5.060e-1541.73Hypothetical protein n=1 Tax=labyrinthulid quahog ... [more]
A0A078B8W1_STYLE1.420e-1428.14Uncharacterized protein n=1 Tax=Stylonychia lemnae... [more]
A0A078ADQ5_STYLE2.090e-1430.05Uncharacterized protein n=1 Tax=Stylonychia lemnae... [more]
J9IX39_9SPIT3.840e-1429.78Uncharacterized protein n=2 Tax=Oxytricha trifalla... [more]
A0A078AZK6_STYLE6.150e-1435.62Uncharacterized protein n=1 Tax=Stylonychia lemnae... [more]
A0A078A232_STYLE9.150e-1430.37Uncharacterized protein n=1 Tax=Stylonychia lemnae... [more]
J9IMW4_9SPIT1.260e-1330.87Uncharacterized protein n=1 Tax=Oxytricha trifalla... [more]
J9F519_9SPIT1.290e-1329.21Uncharacterized protein n=1 Tax=Oxytricha trifalla... [more]
J9IYZ4_9SPIT1.310e-1330.87Uncharacterized protein n=1 Tax=Oxytricha trifalla... [more]
J9HYM5_9SPIT1.310e-1330.72Uncharacterized protein n=1 Tax=Oxytricha trifalla... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig79330contigM-pyrifera_M_contig79330:18..599 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeViridiplantae
Seed ortholog score84.7
Seed ortholog evalue5e-14
Seed eggNOG ortholog3055.EDO98368
Hectar predicted targeting categoryother localisation
EggNOG OGs2CV69@1,2RRDM@2759,34NIE@3041,3831J@33090
Best tax levelChlorophyta
Best eggNOG OGNA|NA|NA
Exons1
Model size582
Cds size573
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816696.72315-CDS-M-pyrifera_M_contig79330:17..5901622816696.72315-CDS-M-pyrifera_M_contig79330:17..590Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig79330 18..590 -
1692278125.319597-CDS-M-pyrifera_M_contig79330:17..5901692278125.319597-CDS-M-pyrifera_M_contig79330:17..590Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig79330 18..590 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816696.7344425-UTR-M-pyrifera_M_contig79330:590..5991622816696.7344425-UTR-M-pyrifera_M_contig79330:590..599Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig79330 591..599 -
1692278125.3320937-UTR-M-pyrifera_M_contig79330:590..5991692278125.3320937-UTR-M-pyrifera_M_contig79330:590..599Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig79330 591..599 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig79330.18626.1prot_M-pyrifera_M_contig79330.18626.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig79330 18..590 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig79330.18626.1

>prot_M-pyrifera_M_contig79330.18626.1 ID=prot_M-pyrifera_M_contig79330.18626.1|Name=mRNA_M-pyrifera_M_contig79330.18626.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=191bp
MNLDTRCTDPDNLALMLSMSLPIIILVRIGFPVASWVFLRSIGPRKLQTL
QWRSRLGFLTNGYRPSLFMWESVVALRKVAFAFVTTTLAPSGGGIQVATA
LLVLIAALVSHATYHPYHTPLLNHLDTISLVTSCITLLGGLFIVLDDAGE
AGLATGIAAPTASVFIVLANSLFFFYGAGLFIYYVYPLISK
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mRNA from alignment at M-pyrifera_M_contig79330:18..599-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig79330.18626.1 ID=mRNA_M-pyrifera_M_contig79330.18626.1|Name=mRNA_M-pyrifera_M_contig79330.18626.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=582bp|location=Sequence derived from alignment at M-pyrifera_M_contig79330:18..599- (Macrocystis pyrifera P11B4 male)
CGCTTAGCCATGAATCTTGATACTCGTTGCACAGACCCAGACAACCTCGC GCTAATGCTTTCCATGTCCCTTCCCATCATCATCCTTGTCCGCATCGGCT TTCCTGTTGCATCTTGGGTCTTTCTCCGCTCCATTGGCCCCAGGAAACTC CAGACTTTGCAATGGCGCAGCCGCCTTGGCTTTCTGACCAATGGCTATCG TCCATCTCTCTTCATGTGGGAGAGTGTCGTGGCGCTCCGCAAAGTTGCCT TTGCCTTTGTCACCACAACCCTCGCACCAAGTGGCGGAGGGATCCAAGTC GCCACCGCACTTCTCGTTCTCATCGCAGCACTTGTCTCCCACGCTACCTA TCACCCCTATCACACTCCCCTCCTCAACCACCTAGACACAATCTCACTCG TTACAAGCTGCATCACACTTCTGGGTGGCCTCTTCATCGTCCTTGACGAC GCTGGAGAGGCAGGGCTTGCTACAGGAATTGCTGCGCCAACGGCCTCTGT ATTCATCGTCCTCGCCAACTCCCTCTTCTTCTTCTATGGTGCTGGTCTTT TCATCTATTACGTCTACCCACTTATCTCCAAA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig79330:18..599-

>mRNA_M-pyrifera_M_contig79330.18626.1 ID=mRNA_M-pyrifera_M_contig79330.18626.1|Name=mRNA_M-pyrifera_M_contig79330.18626.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1146bp|location=Sequence derived from alignment at M-pyrifera_M_contig79330:18..599- (Macrocystis pyrifera P11B4 male)
ATGAATCTTGATACTCGTTGCACAGACCCAGACAACCTCGCGCTAATGCT
TTCCATGTCCCTTCCCATCATCATCCTTGTCCGCATCGGCTTTCCTGTTG
CATCTTGGGTCTTTCTCCGCTCCATTGGCCCCAGGAAACTCCAGACTTTG
CAATGGCGCAGCCGCCTTGGCTTTCTGACCAATGGCTATCGTCCATCTCT
CTTCATGTGGGAGAGTGTCGTGGCGCTCCGCAAAGTTGCCTTTGCCTTTG
TCACCACAACCCTCGCACCAAGTGGCGGAGGGATCCAAGTCGCCACCGCA
CTTCTCGTTCTCATCGCAGCACTTGTCTCCCACGCTACCTATCACCCCTA
TCACACTCCCCTCCTCAACCACCTAGACACAATCTCACTCGTTACAAGCT
GCATCACACTTCTGGGTGGCCTCTTCATCGTCCTTGACGACGCTGGAGAG
GCAGGGCTTGCTACAGGAATTGCTGCGCCAACGGCCTCTGTATTCATCGT
CCTCGCCAACTCCCTCTTCTTCTTCTATGGTGCTGGTCTTTTCATCTATT
ACGTCTACCCACTTATCTCCAAAATGAATCTTGATACTCGTTGCACAGAC
CCAGACAACCTCGCGCTAATGCTTTCCATGTCCCTTCCCATCATCATCCT
TGTCCGCATCGGCTTTCCTGTTGCATCTTGGGTCTTTCTCCGCTCCATTG
GCCCCAGGAAACTCCAGACTTTGCAATGGCGCAGCCGCCTTGGCTTTCTG
ACCAATGGCTATCGTCCATCTCTCTTCATGTGGGAGAGTGTCGTGGCGCT
CCGCAAAGTTGCCTTTGCCTTTGTCACCACAACCCTCGCACCAAGTGGCG
GAGGGATCCAAGTCGCCACCGCACTTCTCGTTCTCATCGCAGCACTTGTC
TCCCACGCTACCTATCACCCCTATCACACTCCCCTCCTCAACCACCTAGA
CACAATCTCACTCGTTACAAGCTGCATCACACTTCTGGGTGGCCTCTTCA
TCGTCCTTGACGACGCTGGAGAGGCAGGGCTTGCTACAGGAATTGCTGCG
CCAACGGCCTCTGTATTCATCGTCCTCGCCAACTCCCTCTTCTTCTTCTA
TGGTGCTGGTCTTTTCATCTATTACGTCTACCCACTTATCTCCAAA
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