mRNA_M-pyrifera_M_contig77571.18254.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig77571.18254.1
Unique NamemRNA_M-pyrifera_M_contig77571.18254.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Match: A0A835ZGN1_9STRA (Splicing factor U2AF subunit n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A835ZGN1_9STRA)

HSP 1 Score: 94.0 bits (232), Expect = 1.290e-20
Identity = 51/99 (51.52%), Postives = 69/99 (69.70%), Query Frame = 1
Query:    1 APTRVLRLSHMVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPTRG-AGMGQVFAHYEDKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRFARQDLA 294
            APTRVL L +MV  +DL+D     DI +D R+E K +G LKTV+IP  G  G+G+VF  Y + + AQAAA A+AGR+F  +I+ A++ DE RFA +DLA
Sbjct:  292 APTRVLVLMNMVGEEDLKDDQEYTDILEDVRDECKQYGELKTVIIPRPGETGVGKVFLEYAEVQQAQAAAMALAGRQFASKIVKAQYHDESRFAARDLA 390          
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Match: A0A8H4EKM5_GIGMA (U2 snRNP auxilliary factor, large subunit, splicing factor n=2 Tax=Gigaspora TaxID=4873 RepID=A0A8H4EKM5_GIGMA)

HSP 1 Score: 73.9 bits (180), Expect = 2.870e-13
Identity = 42/100 (42.00%), Postives = 61/100 (61.00%), Query Frame = 1
Query:    4 PTRVLRLSHMVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPTRGA----GMGQVFAHYEDKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRFARQDL 291
            PT VL+L +MVT ++L D +  EDI DD REE   FG +  + IP  G+    G+G++F  +E  EAA AA +A+AGRKF  R +   + DE+ +  +D 
Sbjct:  569 PTTVLQLLNMVTPEELVDDEEYEDIVDDVREECSKFGRVLDMKIPRPGSASLSGVGKIFVRFESIEAAGAALRALAGRKFAERTVLTSYIDEEVYDAEDF 668          
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Match: A0A137PCH5_CONC2 (Splicing factor U2AF subunit n=1 Tax=Conidiobolus coronatus (strain ATCC 28846 / CBS 209.66 / NRRL 28638) TaxID=796925 RepID=A0A137PCH5_CONC2)

HSP 1 Score: 73.2 bits (178), Expect = 5.250e-13
Identity = 40/101 (39.60%), Postives = 61/101 (60.40%), Query Frame = 1
Query:    4 PTRVLRLSHMVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPT-----RGAGMGQVFAHYEDKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRFARQDL 291
            PTR+L L +MVT ++LED +  E+I  D  EE   FG + ++ IP        + +G+VF  Y++      A K++AGRKF  RI+ A +++ED+FA  DL
Sbjct:  436 PTRILLLLNMVTPEELEDDEEYEEILADIEEEATKFGKVLSIKIPKDKTKYNESAIGKVFIEYDNDTQCAVALKSLAGRKFSDRIVLASYYNEDKFANDDL 536          
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Match: A0A6G0WM79_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WM79_9STRA)

HSP 1 Score: 72.8 bits (177), Expect = 5.780e-13
Identity = 40/102 (39.22%), Postives = 57/102 (55.88%), Query Frame = 1
Query:    4 PTRVLRLSHMVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPTRGA-----GMGQVFAHYEDKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRFARQDLA 294
            PT V++L +MV + +LED D   D+++D  EE   FG +  + IP         G+G+VF  +ED   AQAA KA+ GRKF G I+   +    +F  QD A
Sbjct:  267 PTPVIQLGNMVQASELEDDDEFADLKEDIEEECNKFGKVVALEIPRPNGNETVLGVGKVFVRFEDISGAQAAIKALKGRKFGGNIVEVSYMSLAKFETQDFA 368          
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Match: A0A4S2MPL6_9PEZI (Splicing factor U2AF subunit n=1 Tax=Ascodesmis nigricans TaxID=341454 RepID=A0A4S2MPL6_9PEZI)

HSP 1 Score: 71.6 bits (174), Expect = 1.830e-12
Identity = 41/96 (42.71%), Postives = 59/96 (61.46%), Query Frame = 1
Query:    7 TRVLRLSHMVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPTRGAG------MGQVFAHYEDKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRF 276
            TRVL+L +MVT ++L D +  E+I +D REE   +G L  + IP  G G      +G++F  +ED EAA AA KA+AGRKF  R +   +F E+ +
Sbjct:  449 TRVLQLLNMVTPEELMDNEEYEEICEDIREECSKYGKLVDMKIPRPGGGSKQVVGVGKIFVRFEDVEAANAALKALAGRKFADRTVVCTYFSEENY 544          
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Match: A0A485KWN6_9STRA (Aste57867_12612 protein n=1 Tax=Aphanomyces stellatus TaxID=120398 RepID=A0A485KWN6_9STRA)

HSP 1 Score: 70.5 bits (171), Expect = 4.660e-12
Identity = 38/102 (37.25%), Postives = 57/102 (55.88%), Query Frame = 1
Query:    4 PTRVLRLSHMVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPTRGA-----GMGQVFAHYEDKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRFARQDLA 294
            PT V++L +MV + +LED +   D+++D  EE   FG +  + IP         G+G+VF  +ED   A+AA KA+ GRKF G I+   +    +F  QD A
Sbjct:  446 PTVVIQLGNMVQASELEDDEEFADLKEDIEEECNKFGKVVALEIPRPNGEEAVLGIGKVFVRFEDVSGAEAATKALTGRKFGGNIVDVSYMSLAKFETQDFA 547          
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Match: A0A7S3H7L4_9STRA (Hypothetical protein n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3H7L4_9STRA)

HSP 1 Score: 70.1 bits (170), Expect = 5.810e-12
Identity = 35/100 (35.00%), Postives = 62/100 (62.00%), Query Frame = 1
Query:    4 PTRVLRLSHMVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPTRGAG------MGQVFAHYEDKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRFARQ 285
            P+ V+R+S+M T +DL D D  +++ +D   E  + G +K++VIP   A       +G++F H+ D   A  + +AVAGR+F+GR++ A F+ E+ F ++
Sbjct:  250 PSTVVRMSNMTTDEDLRDDDLFDELTEDVAGECNSHGVVKSIVIPRGPASGPQDTSVGKIFVHFTDIAGATRSRQAVAGRRFNGRVVEAYFYPEELFVKK 349          
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Match: A0A5A8CE56_CAFRO (Uncharacterized protein n=2 Tax=Cafeteria roenbergensis TaxID=33653 RepID=A0A5A8CE56_CAFRO)

HSP 1 Score: 69.3 bits (168), Expect = 1.170e-11
Identity = 38/92 (41.30%), Postives = 56/92 (60.87%), Query Frame = 1
Query:    4 PTRVLRLSHMVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPTRGAGMGQVFAHYEDKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRFA 279
            PT VLR+++M++  +L +P  VE+I  +A       G ++  VIPT G   G VF  + D  AAQAAA+A+ G+ FDGR++   F  E +FA
Sbjct:  388 PTTVLRVANMLSPAELANPAEVEEIRREAMGACGAHGQVQAAVIPTSGPAAGVVFVKFADVRAAQAAAQALGGKTFDGRMLQTSFAPEAQFA 479          
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Match: A0A7S3GNQ6_9STRA (Hypothetical protein n=1 Tax=Spumella elongata TaxID=89044 RepID=A0A7S3GNQ6_9STRA)

HSP 1 Score: 69.3 bits (168), Expect = 1.190e-11
Identity = 42/101 (41.58%), Postives = 57/101 (56.44%), Query Frame = 1
Query:    4 PTRVLRLSHMVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPTRGAGMGQ-----VFAHYEDKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRFARQDL 291
            PTRVLRLS+MVT +DL +     DI++D R E   +G + +VV+P    G        +F  +E  E A AAAK + GRKF   ++H  +F E  FA Q L
Sbjct:  473 PTRVLRLSNMVTREDLYNEAEYVDIKEDVRLECLQYGKVLSVVMPRVKEGYSAAAECLIFVEFEAVEGAMAAAKVLNGRKFAENVVHVAYFSEKDFATQHL 573          
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Match: A0A2G5E9W4_AQUCA (Splicing factor U2af large subunit n=7 Tax=Thalictroideae TaxID=1463137 RepID=A0A2G5E9W4_AQUCA)

HSP 1 Score: 68.6 bits (166), Expect = 2.220e-11
Identity = 38/101 (37.62%), Postives = 60/101 (59.41%), Query Frame = 1
Query:    4 PTRVLRLSHMVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPTRGA------GMGQVFAHYEDKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRFARQD 288
            PT+V+ L+ MV   +L+  +  EDI +D R E + +G L  VVIP          G+G+VF  Y D + A  A + + GRKFDG ++ A F+ ED+F++++
Sbjct:  457 PTKVVCLTQMVNIDELKVDEEYEDIVEDVRSEGEKYGELVNVVIPRPSINGELSPGVGKVFLEYSDTDGATKAKQGLNGRKFDGNLVVAVFYSEDKFSQRE 557          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig77571.18254.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A835ZGN1_9STRA1.290e-2051.52Splicing factor U2AF subunit n=1 Tax=Tribonema min... [more]
A0A8H4EKM5_GIGMA2.870e-1342.00U2 snRNP auxilliary factor, large subunit, splicin... [more]
A0A137PCH5_CONC25.250e-1339.60Splicing factor U2AF subunit n=1 Tax=Conidiobolus ... [more]
A0A6G0WM79_9STRA5.780e-1339.22Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A4S2MPL6_9PEZI1.830e-1242.71Splicing factor U2AF subunit n=1 Tax=Ascodesmis ni... [more]
A0A485KWN6_9STRA4.660e-1237.25Aste57867_12612 protein n=1 Tax=Aphanomyces stella... [more]
A0A7S3H7L4_9STRA5.810e-1235.00Hypothetical protein n=1 Tax=Spumella elongata Tax... [more]
A0A5A8CE56_CAFRO1.170e-1141.30Uncharacterized protein n=2 Tax=Cafeteria roenberg... [more]
A0A7S3GNQ6_9STRA1.190e-1141.58Hypothetical protein n=1 Tax=Spumella elongata Tax... [more]
A0A2G5E9W4_AQUCA2.220e-1137.62Splicing factor U2af large subunit n=7 Tax=Thalict... [more]

Pages

back to top
Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig77571contigM-pyrifera_M_contig77571:342..638 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score75.9
Seed ortholog evalue1.1e-11
Seed eggNOG ortholog159749.K0SQC2
KEGG koko:K12837
KEGG Pathwayko03040,map03040
Hectar predicted targeting categoryother localisation
EggNOG free text desc.RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
EggNOG OGs2XDBR@2836,KOG0120@1,KOG0120@2759
COG Functional cat.A
Best tax levelBacillariophyta
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko03041
Exons1
Model size297
Cds size267
Stop1
Start1
Relationships

The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816676.5025818-UTR-M-pyrifera_M_contig77571:341..3711622816676.5025818-UTR-M-pyrifera_M_contig77571:341..371Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig77571 342..371 +
1692278113.3686986-UTR-M-pyrifera_M_contig77571:341..3711692278113.3686986-UTR-M-pyrifera_M_contig77571:341..371Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig77571 342..371 +


The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816676.5171099-CDS-M-pyrifera_M_contig77571:371..6381622816676.5171099-CDS-M-pyrifera_M_contig77571:371..638Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77571 372..638 +
1692278113.3824496-CDS-M-pyrifera_M_contig77571:371..6381692278113.3824496-CDS-M-pyrifera_M_contig77571:371..638Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig77571 372..638 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig77571.18254.1prot_M-pyrifera_M_contig77571.18254.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig77571 372..638 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig77571.18254.1

>prot_M-pyrifera_M_contig77571.18254.1 ID=prot_M-pyrifera_M_contig77571.18254.1|Name=mRNA_M-pyrifera_M_contig77571.18254.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=89bp
MVTSKDLEDPDTVEDIEDDAREELKNFGSLKTVVIPTRGAGMGQVFAHYE
DKEAAQAAAKAVAGRKFDGRIIHAEFFDEDRFARQDLA*
back to top

mRNA from alignment at M-pyrifera_M_contig77571:342..638+

Legend: UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig77571.18254.1 ID=mRNA_M-pyrifera_M_contig77571.18254.1|Name=mRNA_M-pyrifera_M_contig77571.18254.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=297bp|location=Sequence derived from alignment at M-pyrifera_M_contig77571:342..638+ (Macrocystis pyrifera P11B4 male)
GCTCCAACTAGAGTACTTCGATTGTCACATATGGTCACGAGCAAAGACCT GGAAGACCCTGATACTGTGGAGGATATTGAAGACGACGCGAGAGAGGAAC TCAAAAACTTTGGGTCGCTCAAGACAGTTGTCATCCCCACCAGGGGAGCA GGCATGGGACAAGTCTTTGCGCACTACGAAGATAAAGAAGCCGCACAAGC TGCCGCCAAAGCTGTCGCAGGGAGAAAGTTCGATGGGAGGATTATTCATG CAGAATTCTTTGATGAGGATCGATTTGCAAGACAAGATCTGGCGTAA
back to top

Coding sequence (CDS) from alignment at M-pyrifera_M_contig77571:342..638+

>mRNA_M-pyrifera_M_contig77571.18254.1 ID=mRNA_M-pyrifera_M_contig77571.18254.1|Name=mRNA_M-pyrifera_M_contig77571.18254.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=534bp|location=Sequence derived from alignment at M-pyrifera_M_contig77571:342..638+ (Macrocystis pyrifera P11B4 male)
ATGGTCACGAGCAAAGACCTGGAAGACCCTGATACTGTGGAGGATATTGA
AGACGACGCGAGAGAGGAACTCAAAAACTTTGGGTCGCTCAAGACAGTTG
TCATCCCCACCAGGGGAGCAGGCATGGGACAAGTCTTTGCGCACTACGAA
GATAAAGAAGCCGCACAAGCTGCCGCCAAAGCTGTCGCAGGGAGAAAGTT
CGATGGGAGGATTATTCATGCAGAATTCTTTGATGAGGATCGATTTGCAA
GACAAGATCTGGCGTAAATGGTCACGAGCAAAGACCTGGAAGACCCTGAT
ACTGTGGAGGATATTGAAGACGACGCGAGAGAGGAACTCAAAAACTTTGG
GTCGCTCAAGACAGTTGTCATCCCCACCAGGGGAGCAGGCATGGGACAAG
TCTTTGCGCACTACGAAGATAAAGAAGCCGCACAAGCTGCCGCCAAAGCT
GTCGCAGGGAGAAAGTTCGATGGGAGGATTATTCATGCAGAATTCTTTGA
TGAGGATCGATTTGCAAGACAAGATCTGGCGTAA
back to top