mRNA_M-pyrifera_M_contig75655.17846.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig75655.17846.1
Unique NamemRNA_M-pyrifera_M_contig75655.17846.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Match: A0A2B7ZMV9_9EURO (Adenylyltransferase and sulfurtransferase uba4 n=1 Tax=Emmonsia crescens TaxID=73230 RepID=A0A2B7ZMV9_9EURO)

HSP 1 Score: 152 bits (385), Expect = 8.560e-41
Identity = 78/168 (46.43%), Postives = 109/168 (64.88%), Query Frame = 1 
Query:    1 HRWGGEGEWKVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVLDCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAALPNSSKC--PCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAVILSSSTPSSSTSSTPT 498
            HR    G +KV S  + ++ L+   + + +  HL P++A  I   YDI+LDC+DNPATRYL+SD  V   KPLV  +AL  +GQ+ V NHPP+     +   NS+    PCYRC+FPKPPP A+V SCAD G+IGP  G++G +QAL+TI V+  + T   +T++TPT
Sbjct:  138 HRTRNVGRYKVDSAIEYLKELNPYPKYIPYHTHLTPTDAPSIFHPYDIILDCTDNPATRYLISDTAVLLGKPLVSASALRTEGQLMVLNHPPQKPDKSSTCANSASTGGPCYRCVFPKPPPAASVTSCADGGIIGPVVGVMGVLQALETIRVLTQTLTADKTTTTTPT 305
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BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Match: A0A7L2R282_9PASS (MOCS3 sulfurtransferase (Fragment) n=1 Tax=Oxylabes madagascariensis TaxID=98144 RepID=A0A7L2R282_9PASS)

HSP 1 Score: 142 bits (359), Expect = 4.210e-39
Identity = 71/144 (49.31%), Postives = 97/144 (67.36%), Query Frame = 1 
Query:   19 GEWKVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVLDCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAALPNSSKCPCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAV 450
            G  K RS A ++RRL++ V+ V +   L+P +A+ ++R YD+V DCSDN  TRYLVSDACV A +PLV G+AL L+GQ+AV+NH                 PCYRC+FP+PPPP AV  CAD GV+G  TG++G +QAL+ + +
Sbjct:   70 GRPKARSAAAALRRLNSAVQYVPYRGALRPRSALRLLRQYDVVADCSDNVPTRYLVSDACVLAGRPLVSGSALRLEGQLAVYNH--------------GGGPCYRCLFPRPPPPDAVTDCADGGVLGVVTGVIGCLQALEVLKI 199
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BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Match: A0A674GGT1_TAEGU (Adenylyltransferase and sulfurtransferase MOCS3 n=4 Tax=Passeriformes TaxID=9126 RepID=A0A674GGT1_TAEGU)

HSP 1 Score: 146 bits (369), Expect = 2.880e-38
Identity = 73/144 (50.69%), Postives = 99/144 (68.75%), Query Frame = 1 
Query:   19 GEWKVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVLDCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAALPNSSKCPCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAV 450
            G  K RS A ++RRL++ V+ V +   L+P +A+ ++R YD+V DCSDN ATRYLVSDACV A KPLV G+AL L+GQ+AV+N+                 PCYRC+FP+PPPP AV +C D GV+GP TG++G MQAL+ + +
Sbjct:  243 GRPKARSAAAALRRLNSAVQYVPYRGALRPRSALRLVRQYDVVADCSDNAATRYLVSDACVLAGKPLVSGSALRLEGQLAVYNY--------------RGGPCYRCLFPRPPPPDAVTNCTDGGVLGPVTGVIGCMQALEVLKI 372
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BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Match: UPI0006D4F6C5 (adenylyltransferase and sulfurtransferase MOCS3 n=1 Tax=Halyomorpha halys TaxID=286706 RepID=UPI0006D4F6C5)

HSP 1 Score: 144 bits (363), Expect = 3.640e-38
Identity = 69/146 (47.26%), Postives = 100/146 (68.49%), Query Frame = 1 
Query:   19 GEWKVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVLDCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAALPNSSKCPCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAVIL 456
            G  KVRS AQ++R +++ ++V E+P  L   NA+ IM+ Y +V+D SDNP+TRYLV+DAC+    PLV G+A+G++GQ+ V+N+                 PCYRC+FP+PPPP  V +C D+GV+GP  G++GTMQALQ I ++L
Sbjct:  122 GTSKVRSAAQALRSINSHIKVDEYPVQLNSLNAIQIMQSYSVVIDASDNPSTRYLVNDACIMTKLPLVSGSAVGMEGQLTVYNY--------------KGGPCYRCLFPEPPPPNTVSNCGDAGVLGPVPGVIGTMQALQAIHILL 253
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BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Match: A0A7L2L6M3_9PASS (MOCS3 sulfurtransferase (Fragment) n=2 Tax=Zosterops TaxID=36298 RepID=A0A7L2L6M3_9PASS)

HSP 1 Score: 144 bits (362), Expect = 1.050e-37
Identity = 73/147 (49.66%), Postives = 98/147 (66.67%), Query Frame = 1 
Query:   10 GGEGEWKVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVLDCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAALPNSSKCPCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAV 450
            G  G  K RS A ++RRL++ V+ + +   L P +A+ ++R YDIV DCSDN  TRYLVSDACV A KPLV G+AL L+GQ+AV+NH            N    PCYRC+FP+PPPP  V +CAD GV+G  TG++G +QAL+ + +
Sbjct:  156 GQRGRPKARSAAAALRRLNSAVQYIPYRASLGPRSALRLVRQYDIVADCSDNVPTRYLVSDACVLAGKPLVSGSALRLEGQLAVYNH------------NGG--PCYRCLFPRPPPPDTVTNCADGGVLGAVTGVIGCLQALEVLKI 288
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BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Match: A0A7L2TXJ8_POMRU (MOCS3 sulfurtransferase (Fragment) n=1 Tax=Pomatostomus ruficeps TaxID=9176 RepID=A0A7L2TXJ8_POMRU)

HSP 1 Score: 140 bits (353), Expect = 1.260e-37
Identity = 70/144 (48.61%), Postives = 97/144 (67.36%), Query Frame = 1 
Query:   19 GEWKVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVLDCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAALPNSSKCPCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAV 450
            G  K RS A ++RRL++ V+ V +   L+P +A+ ++R YDIV DCSDN  TRYL+SDACV A +PLV G+AL L+GQ+ V+NH                 PCYRC+FP+PPPP AV +CAD GV+G  TG++G +QAL+ + +
Sbjct:    1 GRPKARSAAAALRRLNSAVQYVPYRGALRPRSALRLVRQYDIVADCSDNAPTRYLLSDACVLAGRPLVSGSALRLEGQLVVYNH--------------GGGPCYRCLFPEPPPPDAVTNCADGGVLGVVTGVIGCLQALEVLKI 130
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BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Match: A0A517L4N8_9PEZI (Adenylyltransferase and sulfurtransferase uba4 n=1 Tax=Venturia effusa TaxID=50376 RepID=A0A517L4N8_9PEZI)

HSP 1 Score: 144 bits (363), Expect = 1.270e-37
Identity = 73/152 (48.03%), Postives = 97/152 (63.82%), Query Frame = 1 
Query:    1 HRWGGEGEWKVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVLDCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAALPNSSKCPCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAVIL 456
            H     G  KV S  Q ++ L++ V  V +   L+P NA+HI+  YD+VLDC+D PA+RYL+SD CV   KPLV  +AL  +GQ+ + N PPR     A  P     PCYRC+FPKPPPP  V SC D G++GP  GI+G +QAL+TI V++
Sbjct:  167 HNEQSNGWAKVDSAMQGLKALNSAVITVPYKTRLEPQNALHILASYDVVLDCTDTPASRYLISDTCVLLGKPLVSASALRTEGQLMLLNCPPR----PAGDPGGG--PCYRCVFPKPPPPETVTSCGDGGILGPVVGIMGLLQALETIKVVI 312
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BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Match: A0A093YWD6_9PEZI (Adenylyltransferase and sulfurtransferase uba4 n=1 Tax=Pseudogymnoascus sp. VKM F-3775 TaxID=1420901 RepID=A0A093YWD6_9PEZI)

HSP 1 Score: 143 bits (361), Expect = 1.380e-37
Identity = 73/145 (50.34%), Postives = 95/145 (65.52%), Query Frame = 1 
Query:   19 GEWKVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVLDCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAALPNSSKCPCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAVI 453
            G WKV S   S++ L+  VE   + EHL P NA +I+ HYDIVLDC+D+P +RYL+SD CV   KPLV  +AL  DGQ+ V NHP       A   ++S  PCYRCIFPKPPP   V SC D G++GP  G++G +QA++ I +I
Sbjct:  137 GLWKVDSAIHSLKGLNPNVEYRAYREHLSPENAANIVTHYDIVLDCTDHPKSRYLISDICVLLQKPLVSASALRTDGQLMVLNHPA------APQGSTSGGPCYRCIFPKPPPANQVVSCGDGGILGPVVGVMGVLQAVEAIKLI 275
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BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Match: A0A8C5NMC1_JUNHY (Adenylyltransferase and sulfurtransferase MOCS3 n=7 Tax=Passeriformes TaxID=9126 RepID=A0A8C5NMC1_JUNHY)

HSP 1 Score: 142 bits (358), Expect = 1.550e-37
Identity = 72/144 (50.00%), Postives = 97/144 (67.36%), Query Frame = 1 
Query:   19 GEWKVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVLDCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAALPNSSKCPCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAV 450
            G  K RS A ++RRL++ V+ V +   L+P +A+ ++R YDIV DCSDN  TRYLVSDACV A +PLV G+AL L+GQ+AV+NH                 PCYRC+FP+PPPP  V +CAD GV+G  TGI+G +QAL+ + +
Sbjct:   98 GRPKARSAAAALRRLNSAVQYVPYRGALRPRSALRLVRQYDIVADCSDNAPTRYLVSDACVLAGRPLVSGSALRLEGQLAVYNH--------------RGGPCYRCLFPRPPPPDTVTNCADGGVLGAVTGIIGCLQALEVLKI 227
[Close]
BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Match: A0A7L2RYW5_9PASS (MOCS3 sulfurtransferase (Fragment) n=1 Tax=Neodrepanis coruscans TaxID=254563 RepID=A0A7L2RYW5_9PASS)

HSP 1 Score: 140 bits (352), Expect = 1.690e-37
Identity = 71/141 (50.35%), Postives = 96/141 (68.09%), Query Frame = 1 
Query:   28 KVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVLDCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAALPNSSKCPCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAV 450
            K RS A ++RRL++ V+ V +   L+P +A+ I++ YDIV DCSDN  TRYLV+DACV A KPLV G+AL L+GQ+AV+NH              +  PCYRC+FP+PPPP  V SCAD GV+G   GI+G +QAL+ + +
Sbjct:    2 KARSAAAALRRLNSAVQYVPYRGALRPRSALRIVQQYDIVADCSDNAPTRYLVNDACVLAGKPLVSGSALRLEGQLAVYNH--------------AGGPCYRCLFPRPPPPHTVTSCADGGVLGVVPGIIGCLQALEVLKI 128
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig75655.17846.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A2B7ZMV9_9EURO8.560e-4146.43Adenylyltransferase and sulfurtransferase uba4 n=1... [more]
A0A7L2R282_9PASS4.210e-3949.31MOCS3 sulfurtransferase (Fragment) n=1 Tax=Oxylabe... [more]
A0A674GGT1_TAEGU2.880e-3850.69Adenylyltransferase and sulfurtransferase MOCS3 n=... [more]
UPI0006D4F6C53.640e-3847.26adenylyltransferase and sulfurtransferase MOCS3 n=... [more]
A0A7L2L6M3_9PASS1.050e-3749.66MOCS3 sulfurtransferase (Fragment) n=2 Tax=Zostero... [more]
A0A7L2TXJ8_POMRU1.260e-3748.61MOCS3 sulfurtransferase (Fragment) n=1 Tax=Pomatos... [more]
A0A517L4N8_9PEZI1.270e-3748.03Adenylyltransferase and sulfurtransferase uba4 n=1... [more]
A0A093YWD6_9PEZI1.380e-3750.34Adenylyltransferase and sulfurtransferase uba4 n=1... [more]
A0A8C5NMC1_JUNHY1.550e-3750.00Adenylyltransferase and sulfurtransferase MOCS3 n=... [more]
A0A7L2RYW5_9PASS1.690e-3750.35MOCS3 sulfurtransferase (Fragment) n=1 Tax=Neodrep... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig75655contigM-pyrifera_M_contig75655:2..499 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeFungi
Seed ortholog score146.0
Seed ortholog evalue1.6e-32
Seed eggNOG ortholog5057.CADACLAP00000958
Preferred namecnxF
KEGG rclassRC00043
KEGG koko:K11996
KEGG ReactionR07459,R07461
KEGG Pathwayko04122,map04122
Hectar predicted targeting categoryother localisation
GOsGO:0000003,GO:0001403,GO:0001558,GO:0002097,GO:0002098,GO:0002143,GO:0003674,GO:0003824,GO:0004792,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0006139,GO:0006396,GO:0006399,GO:0006400,GO:0006464,GO:0006725,GO:0006807,GO:0006950,GO:0006979,GO:0007114,GO:0008033,GO:0008150,GO:0008152,GO:0008641,GO:0009451,GO:0009987,GO:0010467,GO:0010570,GO:0016070,GO:0016740,GO:0016772,GO:0016779,GO:0016782,GO:0016783,GO:0016874,GO:0016877,GO:0018117,GO:0018175,GO:0019538,GO:0019954,GO:0030447,GO:0032446,GO:0032447,GO:0032505,GO:0033554,GO:0034227,GO:0034470,GO:0034599,GO:0034641,GO:0034660,GO:0036211,GO:0036267,GO:0040007,GO:0040008,GO:0042221,GO:0042292,GO:0043170,GO:0043412,GO:0044182,GO:0044237,GO:0044238,GO:0044260,GO:0044267,GO:0044424,GO:0044464,GO:0046483,GO:0050789,GO:0050794,GO:0050896,GO:0051128,GO:0051301,GO:0051716,GO:0065007,GO:0070566,GO:0070647,GO:0070733,GO:0070783,GO:0070784,GO:0070887,GO:0071704,GO:0090304,GO:1900428,GO:1901360,GO:1901564,GO:2000220
EggNOG free text desc.Plays a central role in 2-thiolation of mcm(5)S(2)U at tRNA wobble positions of cytosolic tRNA(Lys), tRNA(Glu) and tRNA(Gln). Also essential during biosynthesis of the molybdenum cofactor. Acts by mediating the C-terminal thiocarboxylation of sulfur carriers urm1 and mocs2a. Its N-terminus first activates urm1 and mocs2a as acyl-adenylates (-COAMP), then the persulfide sulfur on the catalytic cysteine is transferred to urm1 and mocs2a to form thiocarboxylation (-COSH) of their C-terminus. The reaction probably involves hydrogen sulfide that is generated from the persulfide intermediate and that acts as nucleophile towards urm1 and mocs2a. Subsequently, a transient disulfide bond is formed. Does not use thiosulfate as sulfur donor
EggNOG OGs20BGV@147545,38E2S@33154,3NZDJ@4751,3QNXR@4890,3S2RP@5042,COG0476@1,KOG2017@2759
Ec32 ortholog descriptionmolybdopterin biosynthesis protein MoeB
Ec32 orthologEc-09_000960.1
EC2.7.7.80,2.8.1.11
COG Functional cat.H
Best tax levelEurotiales
Best eggNOG OGNA|NA|NA
BRITEko00000,ko00001,ko01000,ko03016,ko04121
Exons1
Model size498
Cds size498
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816654.5311897-CDS-M-pyrifera_M_contig75655:1..4991622816654.5311897-CDS-M-pyrifera_M_contig75655:1..499Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig75655 2..499 +
1692278101.0079753-CDS-M-pyrifera_M_contig75655:1..4991692278101.0079753-CDS-M-pyrifera_M_contig75655:1..499Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig75655 2..499 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig75655.17846.1prot_M-pyrifera_M_contig75655.17846.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig75655 2..499 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig75655.17846.1

>prot_M-pyrifera_M_contig75655.17846.1 ID=prot_M-pyrifera_M_contig75655.17846.1|Name=mRNA_M-pyrifera_M_contig75655.17846.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=166bp
HRWGGEGEWKVRSLAQSIRRLSAVVEVVEFPEHLKPSNAMHIMRHYDIVL
DCSDNPATRYLVSDACVQACKPLVFGAALGLDGQVAVFNHPPRFSQADAA
LPNSSKCPCYRCIFPKPPPPAAVGSCADSGVIGPTTGIVGTMQALQTIAV
ILSSSTPSSSTSSTPT
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mRNA from alignment at M-pyrifera_M_contig75655:2..499+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig75655.17846.1 ID=mRNA_M-pyrifera_M_contig75655.17846.1|Name=mRNA_M-pyrifera_M_contig75655.17846.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=498bp|location=Sequence derived from alignment at M-pyrifera_M_contig75655:2..499+ (Macrocystis pyrifera P11B4 male)
CATCGCTGGGGCGGGGAAGGCGAGTGGAAAGTGCGGTCTTTGGCTCAGAG
CATTCGCAGATTGAGTGCTGTCGTGGAGGTGGTCGAGTTTCCAGAACACT
TGAAACCCTCAAATGCAATGCACATCATGCGACACTATGACATAGTGCTC
GATTGCTCAGACAACCCTGCCACACGCTACCTCGTCTCGGACGCATGTGT
GCAGGCATGCAAACCTCTCGTCTTTGGGGCGGCTCTGGGGCTCGATGGAC
AGGTGGCTGTCTTCAACCACCCTCCACGCTTCTCCCAAGCCGATGCTGCA
TTGCCAAACTCCTCAAAATGCCCATGCTACCGATGCATCTTCCCAAAACC
ACCTCCACCTGCTGCTGTGGGAAGTTGTGCAGACTCTGGAGTCATCGGAC
CAACCACTGGAATCGTAGGAACCATGCAAGCATTGCAAACCATCGCTGTT
ATTCTTTCAAGTTCAACACCGTCATCATCCACCTCCTCAACACCGACA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig75655:2..499+

>mRNA_M-pyrifera_M_contig75655.17846.1 ID=mRNA_M-pyrifera_M_contig75655.17846.1|Name=mRNA_M-pyrifera_M_contig75655.17846.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=996bp|location=Sequence derived from alignment at M-pyrifera_M_contig75655:2..499+ (Macrocystis pyrifera P11B4 male)
CATCGCTGGGGCGGGGAAGGCGAGTGGAAAGTGCGGTCTTTGGCTCAGAG
CATTCGCAGATTGAGTGCTGTCGTGGAGGTGGTCGAGTTTCCAGAACACT
TGAAACCCTCAAATGCAATGCACATCATGCGACACTATGACATAGTGCTC
GATTGCTCAGACAACCCTGCCACACGCTACCTCGTCTCGGACGCATGTGT
GCAGGCATGCAAACCTCTCGTCTTTGGGGCGGCTCTGGGGCTCGATGGAC
AGGTGGCTGTCTTCAACCACCCTCCACGCTTCTCCCAAGCCGATGCTGCA
TTGCCAAACTCCTCAAAATGCCCATGCTACCGATGCATCTTCCCAAAACC
ACCTCCACCTGCTGCTGTGGGAAGTTGTGCAGACTCTGGAGTCATCGGAC
CAACCACTGGAATCGTAGGAACCATGCAAGCATTGCAAACCATCGCTGTT
ATTCTTTCAAGTTCAACACCGTCATCATCCACCTCCTCAACACCGACACA
TCGCTGGGGCGGGGAAGGCGAGTGGAAAGTGCGGTCTTTGGCTCAGAGCA
TTCGCAGATTGAGTGCTGTCGTGGAGGTGGTCGAGTTTCCAGAACACTTG
AAACCCTCAAATGCAATGCACATCATGCGACACTATGACATAGTGCTCGA
TTGCTCAGACAACCCTGCCACACGCTACCTCGTCTCGGACGCATGTGTGC
AGGCATGCAAACCTCTCGTCTTTGGGGCGGCTCTGGGGCTCGATGGACAG
GTGGCTGTCTTCAACCACCCTCCACGCTTCTCCCAAGCCGATGCTGCATT
GCCAAACTCCTCAAAATGCCCATGCTACCGATGCATCTTCCCAAAACCAC
CTCCACCTGCTGCTGTGGGAAGTTGTGCAGACTCTGGAGTCATCGGACCA
ACCACTGGAATCGTAGGAACCATGCAAGCATTGCAAACCATCGCTGTTAT
TCTTTCAAGTTCAACACCGTCATCATCCACCTCCTCAACACCGACA
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