mRNA_M-pyrifera_M_contig7326.17377.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig7326.17377.1
Unique NamemRNA_M-pyrifera_M_contig7326.17377.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Match: A0A6H5J8U9_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5J8U9_9PHAE)

HSP 1 Score: 171 bits (434), Expect = 1.460e-48
Identity = 81/146 (55.48%), Postives = 107/146 (73.29%), Query Frame = 1
Query:  112 YVEVGTKTNVFVSANAYDTRSGQTSGGSGCMDDGCAPALTRDGISD-DVESRWSCNPSIVPDGGLCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESVFNSFDIKGSDVHTLTLTSSGITQNEWISLIEV 546
            +VE G+KT V V+A AYD R G+++G  GC + GC P L  DG+ + D ESRWSC   IVPDGG CEI + F +PQDI  VQV FW+G+ERTRTL+  I+G+ IG ++SYP S FNSF I+ + VHT+T+ S GI +++WISL+EV
Sbjct:  251 FVEAGSKTTVGVTATAYDDRPGESAGDVGCGEMGCLPDLAHDGVGEEDTESRWSCAEEIVPDGGQCEISFTFDSPQDITDVQVAFWKGDERTRTLKVKINGDKIGEYESYPGSTFNSFGIQENGVHTVTMESVGIDRDDWISLLEV 396          
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Match: A0A6H5JFX1_9PHAE (Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2879 RepID=A0A6H5JFX1_9PHAE)

HSP 1 Score: 166 bits (421), Expect = 3.020e-47
Identity = 85/168 (50.60%), Postives = 107/168 (63.69%), Query Frame = 1
Query:   43 LFSICGENAEYKCVDPSAVCVDGYVEVGTKTNVFVSANAYDTRSGQTSGGSGCMDDGCAPALTRDGISDDVESRWSCNPSIVPDGGLCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESVFNSFDIKGSDVHTLTLTSSGITQNEWISLIEV 546
            + +I  ++  Y C D     V+      T T +  SA  +D R GQ SG  GC   GC P L  DGI+DDVESRWSC   IVPDGG CEIE+ F  PQ++K +QV FW+G ERTRTL+  I+GE  G FDS+P SV + FD+   DVHT+TL S G+  +EWISL+EV
Sbjct:  172 VCTIVEDSCHYLCGDMDHELVNA----ATITAIAASATGFDERPGQASGDHGCGKYGCLPVLATDGITDDVESRWSCAEDIVPDGGQCEIEFTFSHPQNLKQLQVDFWKGEERTRTLKVKINGEKFGEFDSHPGSVTSYFDVIAYDVHTVTLESLGLDTDEWISLLEV 335          
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Match: D7G3F6_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G3F6_ECTSI)

HSP 1 Score: 160 bits (405), Expect = 3.380e-46
Identity = 80/147 (54.42%), Postives = 102/147 (69.39%), Query Frame = 1
Query:  115 VEVGTKTNVFVSANAYDTRSGQTSGGSGCMD---DGCAPALTRDGISDDVESRWSCNPSIVPDGGLCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESVFNSFDIKGSDVHTLTLTSSGITQNEWISLIEV 546
            VEVGTKT+V VSA A+DTR G +SG  GC +   +GC PA TRDGIS++ ESRWSC   IVP GG C+IEYVF  PQDI+ +QV FW+G+ER RTLE   DG +  + +SY  S FN   +  + + ++ L S  + +NEWISLIEV
Sbjct:   73 VEVGTKTDVSVSATAHDTRPGSSSGDVGCGEAGGNGCTPANTRDGISNEAESRWSCASKIVPGGGPCQIEYVFGEPQDIEDIQVAFWKGDERVRTLEVYFDGTLYRTHESYAGSTFNPLGVSATGISSVMLESVDLLENEWISLIEV 219          
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Match: A0A6H5JTT4_9PHAE (Uncharacterized protein n=1 Tax=Ectocarpus sp. CCAP 1310/34 TaxID=867726 RepID=A0A6H5JTT4_9PHAE)

HSP 1 Score: 162 bits (410), Expect = 1.770e-43
Identity = 82/147 (55.78%), Postives = 103/147 (70.07%), Query Frame = 1
Query:  115 VEVGTKTNVFVSANAYDTRSGQTSGGSGCMD---DGCAPALTRDGISDDVESRWSCNPSIVPDGGLCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESVFNSFDIKGSDVHTLTLTSSGITQNEWISLIEV 546
            VEVGTKT+V VSA AYDTR G +SG  GC +   +GC+PA TRDGIS++ ESRWSC   IVP GG C+IEYVF  PQDI+ ++V FW G+ER RTLE   DG +  + +SY  S FNS  +  + + T+ L S  + +NEWISLIEV
Sbjct:  479 VEVGTKTDVSVSATAYDTRPGSSSGDVGCGEAGGNGCSPANTRDGISNETESRWSCASKIVPGGGPCQIEYVFGEPQDIEDIKVAFWMGDERVRTLEVYFDGTLHRTHESYAGSTFNSLGVSATGIGTVMLESVDLLENEWISLIEV 625          
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Match: D7FI77_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FI77_ECTSI)

HSP 1 Score: 151 bits (381), Expect = 3.050e-43
Identity = 75/151 (49.67%), Postives = 100/151 (66.23%), Query Frame = 1
Query:  103 VDGYVEVGTKTNVFVSANAYDTRSGQTSGGSGCMDDG---CAPALTRDGISDDVESRWSCNPSIVPDGGLCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESVFNSFDIKGSDVHTLTLTSSGITQNEWISLIEV 546
            VDG VEVGTK  V VSA A+DTR G +SG  GC + G   C    TRDGI  ++ESRWSC   +VPD G C+IE+ F  PQ I  +QV FW+GN+RTRTL   ++GE+  + +SY +S FN+  +  ++  T+ L S+ +  +EWISLIEV
Sbjct:   17 VDGAVEVGTKIEVGVSATAHDTRPGNSSGDVGCGEVGGLGCTATNTRDGIFSEIESRWSCATKLVPDEGPCQIEFTFAEPQHIVDIQVAFWKGNDRTRTLGVHVNGELTHTHESYTDSTFNTLGVTANEASTVMLESTALLSDEWISLIEV 167          
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Match: D7G0S3_ECTSI (LNR domain-containing protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G0S3_ECTSI)

HSP 1 Score: 151 bits (382), Expect = 8.510e-42
Identity = 75/151 (49.67%), Postives = 103/151 (68.21%), Query Frame = 1
Query:  103 VDGYVEVGTKTNVFVSANAYDTRSGQTSGGSGCMD---DGCAPALTRDGISDDVESRWSCNPSIVPDGGLCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESVFNSFDIKGSDVHTLTLTSSGITQNEWISLIEV 546
            VD  VEVGTKT V VSA A+D R+G++SG  GC +   +GCA    RDGIS ++ESRWSC   ++ D G C+IE+ F  PQ+I  +QV FW+GNER RTLE  ++GE+  + +SY +S FN+  +  ++ +T+ L S  +  NEWISLIEV
Sbjct:  150 VDDAVEVGTKTEVSVSATAHDVRAGRSSGEVGCGEAGGNGCAANNARDGISTEIESRWSCAAKLLDDEGPCQIEFSFAEPQNIVDIQVAFWKGNERVRTLEVYVNGELTHTHESYADSTFNTLGVTANEANTVMLESVSLLSNEWISLIEV 300          
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Match: D7G3F4_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G3F4_ECTSI)

HSP 1 Score: 154 bits (388), Expect = 6.450e-41
Identity = 77/151 (50.99%), Postives = 101/151 (66.89%), Query Frame = 1
Query:  103 VDGYVEVGTKTNVFVSANAYDTRSGQTSGGSGCMDDG---CAPALTRDGISDDVESRWSCNPSIVPDGGLCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESVFNSFDIKGSDVHTLTLTSSGITQNEWISLIEV 546
            VDG VEVGTKT V VS+ A+D R G +SG +GC + G   C    TRDGI  ++ESRWSC   +V D G C+IE+ F  PQ I  +QV FW+GNERTRTLE  I+GE+  + +SY ++ FN+  +  +DV T+ L S  +  +EWISLIEV
Sbjct:  359 VDGAVEVGTKTEVGVSSTAHDVRPGMSSGDAGCGEVGGLGCTATNTRDGIFSEIESRWSCATKLVDDEGPCQIEFTFAEPQTIMDIQVAFWKGNERTRTLEVHINGEMTHTHESYADATFNTLGVTATDVSTVMLESVALLSDEWISLIEV 509          
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Match: D7G5F4_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7G5F4_ECTSI)

HSP 1 Score: 146 bits (368), Expect = 1.770e-40
Identity = 76/151 (50.33%), Postives = 98/151 (64.90%), Query Frame = 1
Query:  103 VDGYVEVGTKTNVFVSANAYDTRSGQTSGGSGCMDDG---CAPALTRDGISDDVESRWSCNPSIVPDGGLCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESVFNSFDIKGSDVHTLTLTSSGITQNEWISLIEV 546
            VDG VEVGTKT V VS+ A+D R G      GC + G   C  A TRDGI  ++ESRWSC   +V D G C+IE+ F  PQ I  +QV FW+GNERTRTLE  I+GE+  + +SY ++ FN+  +  +DV T+ L S  +  +EWISLIEV
Sbjct:   85 VDGAVEVGTKTEVGVSSTAHDVRPGDD----GCGEVGGLGCTAANTRDGIFSEIESRWSCATKLVDDEGPCQIEFTFAEPQTIVDIQVAFWKGNERTRTLEVYINGEMTHTHESYADATFNTLGVTATDVSTVMLESVALLSDEWISLIEV 231          
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Match: D7FI81_ECTSI (Uncharacterized protein n=1 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D7FI81_ECTSI)

HSP 1 Score: 147 bits (370), Expect = 5.650e-40
Identity = 73/146 (50.00%), Postives = 96/146 (65.75%), Query Frame = 1
Query:  118 EVGTKTNVFVSANAYDTRSGQTSGGSGCMD---DGCAPALTRDGISDDVESRWSCNPSIVPDGGLCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESVFNSFDIKGSDVHTLTLTSSGITQNEWISLIEV 546
            EVGT T V VSA A+DTR G +SG  GC +   DGCA     DGI  ++ESRWSC   +VPD G C+IE+ F  PQDI  +QV FW GNERTRT++  IDGE+  + +SY +S FN+  +  ++  T+ L S  +  +EWISL+EV
Sbjct:  158 EVGTMTEVNVSATAHDTRPGSSSGDVGCGETGGDGCAAIKAHDGIISEIESRWSCATMLVPDEGPCQIEFSFADPQDIVDIQVAFWEGNERTRTVDIFIDGELTHTHESYADSTFNTLGVTATEASTVMLESVDLLSDEWISLLEV 303          
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Match: D8LCQ5_ECTSI (Uncharacterized protein n=2 Tax=Ectocarpus siliculosus TaxID=2880 RepID=D8LCQ5_ECTSI)

HSP 1 Score: 138 bits (348), Expect = 5.980e-39
Identity = 64/113 (56.64%), Postives = 82/113 (72.57%), Query Frame = 1
Query:  211 GCAPALTRDGISD-DVESRWSCNPSIVPDGGLCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESVFNSFDIKGSDVHTLTLTSSGITQNEWISLIEV 546
            GC P L  DG+ + D ESRWSC   IVPDGG CEI + F +PQDI  VQV FW+G+ERTRTL+  I+G+ IG ++SYP S FNSF I+ + VHT+ + S GI  ++WISL+EV
Sbjct:    2 GCLPDLAHDGVGEEDTESRWSCAEEIVPDGGQCEITFTFDSPQDITDVQVAFWKGDERTRTLKVKINGDKIGEYESYPGSTFNSFGIQENGVHTVAMESVGIDADDWISLLEV 114          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig7326.17377.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 22
Match NameE-valueIdentityDescription
A0A6H5J8U9_9PHAE1.460e-4855.48Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
A0A6H5JFX1_9PHAE3.020e-4750.60Uncharacterized protein n=2 Tax=Ectocarpus TaxID=2... [more]
D7G3F6_ECTSI3.380e-4654.42Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
A0A6H5JTT4_9PHAE1.770e-4355.78Uncharacterized protein n=1 Tax=Ectocarpus sp. CCA... [more]
D7FI77_ECTSI3.050e-4349.67Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7G0S3_ECTSI8.510e-4249.67LNR domain-containing protein n=1 Tax=Ectocarpus s... [more]
D7G3F4_ECTSI6.450e-4150.99Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7G5F4_ECTSI1.770e-4050.33Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D7FI81_ECTSI5.650e-4050.00Uncharacterized protein n=1 Tax=Ectocarpus silicul... [more]
D8LCQ5_ECTSI5.980e-3956.64Uncharacterized protein n=2 Tax=Ectocarpus silicul... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig7326contigM-pyrifera_M_contig7326:9515..10546 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score98.2
Seed ortholog evalue4.3e-18
Seed eggNOG ortholog2880.D7FL48
Hectar predicted targeting categoryother localisation
EggNOG free text desc.regulation of response to stimulus
EggNOG OGsCOG4886@1,KOG0619@2759
COG Functional cat.S
Best tax levelEukaryota
Best eggNOG OGNA|NA|NA
Exons2
Model size567
Cds size567
Stop1
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816629.5910265-CDS-M-pyrifera_M_contig7326:9514..99101622816629.5910265-CDS-M-pyrifera_M_contig7326:9514..9910Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7326 9515..9910 +
1692278086.9048848-CDS-M-pyrifera_M_contig7326:9514..99101692278086.9048848-CDS-M-pyrifera_M_contig7326:9514..9910Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7326 9515..9910 +
1622816629.6020687-CDS-M-pyrifera_M_contig7326:10375..105461622816629.6020687-CDS-M-pyrifera_M_contig7326:10375..10546Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7326 10376..10546 +
1692278086.92069-CDS-M-pyrifera_M_contig7326:10375..105461692278086.92069-CDS-M-pyrifera_M_contig7326:10375..10546Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig7326 10376..10546 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig7326.17377.1prot_M-pyrifera_M_contig7326.17377.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig7326 9515..10546 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig7326.17377.1

>prot_M-pyrifera_M_contig7326.17377.1 ID=prot_M-pyrifera_M_contig7326.17377.1|Name=mRNA_M-pyrifera_M_contig7326.17377.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=189bp
YDGGDCCECTCISGLFSICGENAEYKCVDPSAVCVDGYVEVGTKTNVFVS
ANAYDTRSGQTSGGSGCMDDGCAPALTRDGISDDVESRWSCNPSIVPDGG
LCEIEYVFQAPQDIKSVQVVFWRGNERTRTLEAAIDGEVIGSFDSYPESV
FNSFDIKGSDVHTLTLTSSGITQNEWISLIEVSPACTQ*
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mRNA from alignment at M-pyrifera_M_contig7326:9515..10546+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig7326.17377.1 ID=mRNA_M-pyrifera_M_contig7326.17377.1|Name=mRNA_M-pyrifera_M_contig7326.17377.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=1032bp|location=Sequence derived from alignment at M-pyrifera_M_contig7326:9515..10546+ (Macrocystis pyrifera P11B4 male)
TACGACGGAGGGGATTGCTGCGAGTGCACCTGCATCAGTGGCTTGTTCTC TATCTGCGGAGAGAACGCCGAGTACAAATGCGTCGACCCGAGCGCCGTGT GTGTCGACGGCTACGTCGAGGTCGGGACCAAGACCAACGTGTTTGTGTCC GCCAACGCCTACGACACGCGCTCCGGCCAAACTTCTGGTGGCAGTGGATG CATGGATGATGGGTGCGCGCCCGCGCTAACCCGAGACGGCATCAGTGACG ACGTAGAATCGAGATGGTCCTGTAACCCAAGCATTGTCCCCGACGGAGGG CTGTGCGAGATCGAGTATGTGTTCCAAGCCCCTCAGGACATCAAGAGCGT CCAGGTGGTATTCTGGAGGGGGAACGAGCGGACACGCACGCTGGAGGTAC GCCCCGTAGAGCGGAACGTTTTCCCGGTGTACTGTCGCGGTAGGGCTTCT CTTTCACGGCCTTCTGGGTTGCTTTGAGTACGAGGCCCGTCACTTGACGC TTTCATTTGACCGCATGTAACGACGGATCATGCAATTTTGGTGTTACGAA GTGGCTCGATAACCCAAGGGTGTTCCATGCTGAACCGTGTGTTCGCTTGT GGTGGCACAGTCCGAAACGTGTGCGTAGGGTTTGTATTCGTACCCTGCAG ACAAGATACTTGAACGGTGGAAAAAGAAGTAGGAAGAACGCCGAGAACGC TGTAAGGCACCGTTTTTTGCTACTTGGTCCAGCGATACCGTGAAGGTTTG ACCCAAGCACAGAGCATCGGGCGACGACCGAGCCTTCGACGTAAACCTGA TCGTCGTATCCGTTCGACAATGCACCCCACAACATTCCTCTGAACGTTCT GTTGTGTTCAGGCTGCGATCGACGGCGAGGTCATCGGTAGTTTCGATTCC TACCCGGAGTCGGTCTTCAACTCGTTCGACATCAAGGGAAGCGATGTACA CACGCTGACGCTCACGTCTAGCGGCATCACCCAAAACGAATGGATTAGCT TGATTGAGGTAAGTCCCGCATGCACGCAATGA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig7326:9515..10546+

>mRNA_M-pyrifera_M_contig7326.17377.1 ID=mRNA_M-pyrifera_M_contig7326.17377.1|Name=mRNA_M-pyrifera_M_contig7326.17377.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=1134bp|location=Sequence derived from alignment at M-pyrifera_M_contig7326:9515..10546+ (Macrocystis pyrifera P11B4 male)
TACGACGGAGGGGATTGCTGCGAGTGCACCTGCATCAGTGGCTTGTTCTC
TATCTGCGGAGAGAACGCCGAGTACAAATGCGTCGACCCGAGCGCCGTGT
GTGTCGACGGCTACGTCGAGGTCGGGACCAAGACCAACGTGTTTGTGTCC
GCCAACGCCTACGACACGCGCTCCGGCCAAACTTCTGGTGGCAGTGGATG
CATGGATGATGGGTGCGCGCCCGCGCTAACCCGAGACGGCATCAGTGACG
ACGTAGAATCGAGATGGTCCTGTAACCCAAGCATTGTCCCCGACGGAGGG
CTGTGCGAGATCGAGTATGTGTTCCAAGCCCCTCAGGACATCAAGAGCGT
CCAGGTGGTATTCTGGAGGGGGAACGAGCGGACACGCACGCTGGAGTACG
ACGGAGGGGATTGCTGCGAGTGCACCTGCATCAGTGGCTTGTTCTCTATC
TGCGGAGAGAACGCCGAGTACAAATGCGTCGACCCGAGCGCCGTGTGTGT
CGACGGCTACGTCGAGGTCGGGACCAAGACCAACGTGTTTGTGTCCGCCA
ACGCCTACGACACGCGCTCCGGCCAAACTTCTGGTGGCAGTGGATGCATG
GATGATGGGTGCGCGCCCGCGCTAACCCGAGACGGCATCAGTGACGACGT
AGAATCGAGATGGTCCTGTAACCCAAGCATTGTCCCCGACGGAGGGCTGT
GCGAGATCGAGTATGTGTTCCAAGCCCCTCAGGACATCAAGAGCGTCCAG
GTGGTATTCTGGAGGGGGAACGAGCGGACACGCACGCTGGAGGCTGCGAT
CGACGGCGAGGTCATCGGTAGTTTCGATTCCTACCCGGAGTCGGTCTTCA
ACTCGTTCGACATCAAGGGAAGCGATGTACACACGCTGACGCTCACGTCT
AGCGGCATCACCCAAAACGAATGGATTAGCTTGATTGAGGTAAGTCCCGC
ATGCACGCAATGAGCTGCGATCGACGGCGAGGTCATCGGTAGTTTCGATT
CCTACCCGGAGTCGGTCTTCAACTCGTTCGACATCAAGGGAAGCGATGTA
CACACGCTGACGCTCACGTCTAGCGGCATCACCCAAAACGAATGGATTAG
CTTGATTGAGGTAAGTCCCGCATGCACGCAATGA
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