prot_M-pyrifera_M_contig72997.17331.1 (polypeptide) Macrocystis pyrifera P11B4 male

You are viewing a polypeptide, more information available on the corresponding mRNA page

Overview
NamemRNA_M-pyrifera_M_contig72997.17331.1
Unique Nameprot_M-pyrifera_M_contig72997.17331.1
Typepolypeptide
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Sequence length247
Homology
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Match: A0A8J5QS52_9HYME (Uncharacterized protein n=1 Tax=Cotesia typhae TaxID=2053667 RepID=A0A8J5QS52_9HYME)

HSP 1 Score: 194 bits (492), Expect = 6.570e-58
Identity = 105/238 (44.12%), Postives = 142/238 (59.66%), Query Frame = 0
Query:    9 LMMMDDEGVSSTTSVSQMELEELCARYWAFPSSDVALHRDNHERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRRVLLEDLQSPTGGFCGGVGQEAHAAPTYSAVLALAVLSEEDGVEGLDREGLVSFFRRLKAKSGGFAMHVGGEMDVRATYTVVAVSWLTGVLGEEGLMDGVAEYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGRWDVVDVDA 246
            L+  DDE   +TTSV Q  +EE C + +   + D  L R  H  +L + L + S   + LD SRPWL YW +H L+LLG +    +  K+   L +  Q P GGF GG GQ  H A TY+AV AL  +  +   + +DR+ LV FFR LK K G F+MH  GE+D+R  Y  +AV+ LT V  EE L  G  ++I  CQT+EGGFGG PG EAHGGYT+  +A+L L+G+ D  ++ A
Sbjct:   21 LVTQDDEDFETTTSVEQEIVEESCLQLYKI-AEDPKLSRGRHISWLKKSLHKLSEGYECLDCSRPWLCYWSLHALNLLGEKLNEEDNTKVIDFLSK-CQDPLGGFGGGPGQFPHLASTYAAVCALCNIKSKKAYDAIDRKSLVRFFRSLKFKDGSFSMHRDGELDIRGVYCAIAVAKLTNVYSEE-LFKGTDDWISRCQTWEGGFGGCPGMEAHGGYTYCGLASLVLMGKVDKCNLSA 255          
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Match: A0A8J6CD53_DIALT (Protein farnesyltransferase subunit beta n=1 Tax=Diacronema lutheri TaxID=2081491 RepID=A0A8J6CD53_DIALT)

HSP 1 Score: 198 bits (504), Expect = 8.600e-58
Identity = 112/238 (47.06%), Postives = 144/238 (60.50%), Query Frame = 0
Query:   17 VSSTTSVSQMELEELCARYW--------AFPSSDVALHRDNHERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRRVL--LEDLQSPTGGFCGGV--GQEAHAAPTYSAVLALAVLSEEDGVEGLDREGLVSFFRRLKAKSGGFAMHVGGEMDVRATYTVVAVSWLTGVLGEEGLMDGVAEYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGRWDVV 242
            +S+ T+  Q  +EE C R +        A    +V L R+ H RYL RGL R S   Q LD+SRPWL YW +HGLDLLG     ++ E   R++  L+  Q P GG+CGG   GQ  H APTY+AV AL ++  ++ +  +DR  L +F +  K +SG + MH  GE+DVR  YT +AV+ LT +L  E L    AE+I  CQTYEGG GGEPG EAHGGYTF   AAL LLGR D +
Sbjct:   16 LSTVTTQQQASVEENCLRLYMRHLGRTNAVQRDEVHLAREEHVRYLLRGLERLSSGFQVLDSSRPWLCYWILHGLDLLGH---DLDAELGSRIVGFLKRCQDPAGGYCGGPYPGQLPHLAPTYAAVNALVIIGTDEALASIDRPALHAFLKGRKHESGYYTMHEDGEIDVRGAYTAIAVATLTNLLSNE-LTARTAEWIATCQTYEGGLGGEPGLEAHGGYTFCGTAALSLLGRLDAL 249          
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Match: UPI0014255204 (protein farnesyltransferase subunit beta-like n=1 Tax=Anneissia japonica TaxID=1529436 RepID=UPI0014255204)

HSP 1 Score: 196 bits (498), Expect = 3.670e-57
Identity = 107/238 (44.96%), Postives = 143/238 (60.08%), Query Frame = 0
Query:   15 EGVSSTTSVSQMELE----ELCARYWAFPSSDVALHRDNHERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRRV--LLEDLQSPTGGFCGGVGQEAHAAPTYSAVLALAVLSEEDGVEGLDREGLVSFFRRLKAKSGGFAMHVGGEMDVRATYTVVAVSWLTGVLGEEGLMDGVAEYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGRWDVVDVDA 246
            +G  + +S+ Q ++E    E+  ++         L RD H  YL++GL   S S Q LDASRPWL YW +H L+LLG +   +  E   +V   +   Q P GGF GG GQ AH APTY+AVLALA +  ++  E ++RE L SF  R+++  GGF MH GGE+DVR  Y     + LT +   E + +G  E+I +CQTYEGGF G PG EAHGGYTF AVAAL L+G   + D +A
Sbjct:   21 DGYHTNSSIEQEKVEAKIGEIFEKFATSSMKIPKLQRDKHLAYLNKGLKNLSESYQCLDASRPWLCYWILHSLELLGEK---ISEEHASQVAQFIGKCQHPEGGFAGGPGQYAHLAPTYAAVLALATIGTKEAYEVVNREKLASFLMRMRSPDGGFLMHEGGEIDVRGAYCAAVAARLTNIATTE-MFEGTPEWIASCQTYEGGFAGIPGMEAHGGYTFCAVAALVLMGHDRLCDTEA 254          
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Match: UPI0019393F96 (protein farnesyltransferase subunit beta-like n=1 Tax=Styela clava TaxID=7725 RepID=UPI0019393F96)

HSP 1 Score: 194 bits (493), Expect = 2.540e-56
Identity = 106/241 (43.98%), Postives = 143/241 (59.34%), Query Frame = 0
Query:   13 DDEGVSSTTSVSQMELEELCAR----YWAFPSSDVALH--RDNHERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRRVLLEDLQSPTGGFCGGVGQEAHAAPTYSAVLALAVLSEEDGVEGLDREGLVSFFRRLKAKSGGFAMHVGGEMDVRATYTVVAVSWLTGV--LGEEGLMDGVAEYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGRWDVVDVD 245
            DDEG  + T+V Q  +EE   R    +  F   +  +H  RD+H  YL +GL R S   + LDAS+PWL YW VH L LLG      ++  + + L E  QSPTGG+ GG  Q AH APTY+AV  L +++ +D  E ++RE L+ F  +++   G F MH GGE D R+ Y  ++V+ LTG+  +  E L  G  +YI +CQTYEGG GG PG EAHGGYTF   A L +LG   ++D D
Sbjct:   18 DDEGTPTKTTVEQQRVEESVLRKLKDFEDFSDLEPGIHLKRDDHLPYLLKGLERLSEGYECLDASQPWLCYWIVHSLSLLGHELSEQQKSSVVKFL-EKCQSPTGGYGGGPEQFAHLAPTYAAVNCLCIIATDDAYESINREKLLYFLFQMRQNDGSFTMHEGGESDTRSLYCAISVASLTGLDEIAGESLFAGSPQYIVSCQTYEGGIGGCPGAEAHGGYTFCGYAGLVILGHEKLIDTD 257          
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Match: F0Y5G6_AURAN (Protein farnesyltransferase subunit beta n=1 Tax=Aureococcus anophagefferens TaxID=44056 RepID=F0Y5G6_AURAN)

HSP 1 Score: 192 bits (488), Expect = 3.640e-56
Identity = 108/204 (52.94%), Postives = 129/204 (63.24%), Query Frame = 0
Query:   45 LHRDNHERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRRVL--LEDLQSPTGGFCGGVGQEAHAAPTYSAVLALAVLSEEDGVEGLDREGLVSFFRRLKAKSGGFAMHVGGEMDVRATYTVVAVSWLTGVLGEEGLMDGVAEYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGRWDVVDVDA 246
            L R+ H  YL +GL     S   LDASRPWL YW +HG+DLL   P     EK+   +  L   +SPTGG+ GG  Q AH APTY+A LA+AVL      E +DR+GL +F   +K  SGGF MH  GE+DVR TYT +AV+ L  VL  E L +G A Y   CQTYEGGFGGEPG EAHGGY F A+AAL +L   D VD+DA
Sbjct:   10 LMRERHVAYLMKGLGGLGGSYVALDASRPWLCYWILHGMDLLDALP----EEKIDDCVATLAKCRSPTGGYGGGPQQLAHCAPTYAASLAIAVLGTRRAYESVDRKGLYAFLLSMKDPSGGFRMHDDGEVDVRGTYTALAVAALFNVLTPE-LAEGAAAYALRCQTYEGGFGGEPGVEAHGGYVFCALAALVILNATDAVDLDA 208          
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Match: A0A7S2TFR8_9EUKA (Protein farnesyltransferase subunit beta n=1 Tax=Lotharella oceanica TaxID=641309 RepID=A0A7S2TFR8_9EUKA)

HSP 1 Score: 195 bits (495), Expect = 4.440e-56
Identity = 103/239 (43.10%), Postives = 148/239 (61.92%), Query Frame = 0
Query:   15 EGVSSTTSVSQMELEELCARYWAFP-SSDVALHRDNHERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRRV--LLEDLQSPTGGFCGGVGQEAHAAPTYSAVLALAVLSEEDGVEGLDREGLVSFFRRLKAKSG----GFAMHVGGEMDVRATYTVVAVSWLTGVLGEEGLMDGVAEYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGRWDVVDVDA 246
            + + + +S+ Q  LE LC        +S + L R+ H+ YL  GL         LDASRPW+++W +H L+LLG        E  RRV   L+  Q+P+GGF GG GQ AH APTY+A+++L +L  E+G + ++R+ +  F   +K KSG    GFA+ VGGE+D R TYT +AV+ +  ++  E L+DGVAE++   QTYEGG G EPGNEAHGGY +  +AAL ++ R D +D+ A
Sbjct:   34 DNIETASSLEQRRLERLCTDLLTRDEASPIELLRNFHQSYLVHGLTHLPGGYSSLDASRPWILFWILHSLELLGALDKLPSDEDARRVYAFLDRCQAPSGGFGGGPGQRAHLAPTYAAIMSLVILGSENGFKIVNRQKMYEFLMSVKVKSGILRGGFAVTVGGEVDTRGTYTSLAVASILNIITPE-LVDGVAEFVARNQTYEGGIGAEPGNEAHGGYAYCGLAALRIINRTDAIDLPA 271          
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Match: A0A197K9B1_9FUNG (Protein farnesyltransferase subunit beta n=1 Tax=Linnemannia elongata AG-77 TaxID=1314771 RepID=A0A197K9B1_9FUNG)

HSP 1 Score: 192 bits (489), Expect = 1.670e-55
Identity = 107/243 (44.03%), Postives = 148/243 (60.91%), Query Frame = 0
Query:   13 DDEGVSSTTSVSQMELEELCARYW---------AFPSSDVALHRDNHERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRRV-LLEDLQSPTGGFCGGVGQEAHAAPTYSAVLALAVLSEEDGVEGLDREGLVSFFRRLKAKSGGFAMHVGGEMDVRATYTVVAVSWLTGVLGEEGLMDGVAEYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGRWDVVDVD 245
            +D+GV + +S  Q+E EE   R +           P + + L +  H +Y+   L R +     LDAS+PWL YW +H LDLLG R    ER  LR +  ++ +QSPTGGF GG GQEAH A TY+AV  LA++  ++  + ++RE L  F   +K K G F M VGGE+DVR +Y  ++ + +T ++  E L    AE+I  CQTYEGG GG PG EAH GYTF  +AALELLGR D++++D
Sbjct:   16 NDDGVETDSSEVQLETEESIRRIFFPYTQESPMPLPIASLELRKLRHVKYVLGALDRLASHWTALDASKPWLCYWILHALDLLGYRIP--ERLALRAISTMQHIQSPTGGFGGGPGQEAHLATTYAAVNTLAIIGTKEAYDVINRETLKDFLMSVKQKDGSFTMTVGGEVDVRGSYCAMSAATITNIVTPE-LTANAAEFIARCQTYEGGIGGFPGVEAHAGYTFCGLAALELLGRTDILNLD 255          
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Match: UPI001BB1FD65 (protein farnesyltransferase subunit beta-like n=1 Tax=Lytechinus variegatus TaxID=7654 RepID=UPI001BB1FD65)

HSP 1 Score: 192 bits (487), Expect = 1.890e-55
Identity = 108/241 (44.81%), Postives = 141/241 (58.51%), Query Frame = 0
Query:   14 DEGVSSTTSVSQMELEELCARYWAFPSSDV----------ALHRDNHERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRRVLLEDLQSPTGGFCGGVGQEAHAAPTYSAVLALAVLSEEDGVEGLDREGLVSFFRRLKAKSGGFAMHVGGEMDVRATYTVVAVSWLTGVLGEEGLMDGVAEYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGRWDVVDV 244
            D+GV ++TS  Q ++E L    +A   + +           L R  H  YL++GL   S S + LDASRPWL YW +H L+LLG +  P +   + + L +  Q P GGF GG GQ AH APTY+AVLAL+ L  E+    +DR  L S+  R+K   G F MH  GE+DVR  Y     + LT V   + + DG  E+I +CQTYEGGF G+PG EAHGGYTF +VAAL LLG   + DV
Sbjct:   20 DDGVPTSTSEQQEDVENLVDNKFAMYRARIKNLAPEDKGPVLERFKHLAYLNKGLCYLSESYEVLDASRPWLCYWILHSLELLGEKLTPEQSSNIAQFLGK-CQCPDGGFAGGPGQIAHLAPTYAAVLALSTLGTEEAYNIIDRPKLQSYLLRMKTPEGAFLMHSSGEVDVRGAYCAAVSALLTNVATPD-MFDGTPEWIVSCQTYEGGFAGQPGMEAHGGYTFCSVAALVLLGHERLCDV 258          
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Match: A0A836CMB6_9STRA (Protein farnesyltransferase subunit beta n=1 Tax=Tribonema minus TaxID=303371 RepID=A0A836CMB6_9STRA)

HSP 1 Score: 194 bits (492), Expect = 4.010e-55
Identity = 113/257 (43.97%), Postives = 138/257 (53.70%), Query Frame = 0
Query:    3 MPEYVELMMMDDEGVSSTTSVSQMELEELCA---------------------RYWAFPSSDVALHRDNHERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRRVLLEDLQSPTGGFCGGVGQEAHAAPTYSAVLALAVLSEEDGVEGLDREGLVSFFRRLKAKSGGFAMHVGGEMDVRATYTVVAVSWLTGVLGEEGLMDGVAEYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGR 238
            M EY + + +  +G  + T+V Q E E  C                      R     S D+ L R  H  YL +GL         LDASRPW++YW  H LDLL       +   +    L   Q PTGGF GG  Q AH APTY+AVL L +L  E     +DR  L  FF  +K  SGGF MH  GE+DVR TYTV++++ L  +L  E L+ GVAEY+ A QTYEGGFGGEPGNEAHGGY F A A L +LGR
Sbjct:    1 MEEYYKRLEVQTDGCPTRTTVDQAECERTCTVSMLPLHMDDLSDQEVLEFQNRGILNESGDIVLMRAKHIAYLRKGLKVLPAGFVALDASRPWIIYWITHSLDLLNALRSDDDAAII--ATLASCQQPTGGFGGGPQQLAHCAPTYAAVLTLLLLGTEAAYSAVDRSALYRFFTSIKHPSGGFRMHEDGEVDVRGTYTVISIAALLNILTPE-LVQGVAEYVLAAQTYEGGFGGEPGNEAHGGYAFCAFATLGILGR 254          
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Match: A0A6P8IMP0_ACTTE (Protein farnesyltransferase subunit beta n=1 Tax=Actinia tenebrosa TaxID=6105 RepID=A0A6P8IMP0_ACTTE)

HSP 1 Score: 191 bits (484), Expect = 4.040e-55
Identity = 104/236 (44.07%), Postives = 141/236 (59.75%), Query Frame = 0
Query:   14 DEGVSSTTSVSQMELEELCARYWAFP------SSDVALHRDNHERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRRVLLEDLQSPTGGFCGGVGQEAHAAPTYSAVLALAVLSEEDGVEGLDREGLVSFFRRLKAKSGGFAMHVGGEMDVRATYTVVAVSWLTGVLGEEGLMDGVAEYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGRWDVVD 243
            D+G+ + TS  Q  +EE+ ++ +A        + D  L+R  H  YL RGL   S S + LDASRPWL YW +H L+LL     P     +   L    Q P GGF GG GQ  H APTY+AV AL++L  ++ ++ LDR+GL +F  + K+  G F MHV GE+D+R  Y  V V+ +T ++    L +G  E++  CQTYEGGF GEPG EAHGGYTF   AAL LLG+  ++D
Sbjct:   16 DDGLRTVTSEEQQRVEEVVSKNYASYLRVADLNPDFPLYRQKHLHYLRRGLRHLSDSYECLDASRPWLCYWILHSLELLDEHVPPQVTSNIVDFLRR-CQDPEGGFGGGPGQVPHLAPTYAAVCALSILGSKEALDILDRKGLQNFLSQCKSIDGSFRMHVDGEVDIRGVYCAVVVASITNIMTPS-LFEGTDEWLTKCQTYEGGFCGEPGLEAHGGYTFCGYAALILLGKEHLID 249          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig72997.17331.1 vs. uniprot
Analysis Date: 2022-09-16 (Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
A0A8J5QS52_9HYME6.570e-5844.12Uncharacterized protein n=1 Tax=Cotesia typhae Tax... [more]
A0A8J6CD53_DIALT8.600e-5847.06Protein farnesyltransferase subunit beta n=1 Tax=D... [more]
UPI00142552043.670e-5744.96protein farnesyltransferase subunit beta-like n=1 ... [more]
UPI0019393F962.540e-5643.98protein farnesyltransferase subunit beta-like n=1 ... [more]
F0Y5G6_AURAN3.640e-5652.94Protein farnesyltransferase subunit beta n=1 Tax=A... [more]
A0A7S2TFR8_9EUKA4.440e-5643.10Protein farnesyltransferase subunit beta n=1 Tax=L... [more]
A0A197K9B1_9FUNG1.670e-5544.03Protein farnesyltransferase subunit beta n=1 Tax=L... [more]
UPI001BB1FD651.890e-5544.81protein farnesyltransferase subunit beta-like n=1 ... [more]
A0A836CMB6_9STRA4.010e-5543.97Protein farnesyltransferase subunit beta n=1 Tax=T... [more]
A0A6P8IMP0_ACTTE4.040e-5544.07Protein farnesyltransferase subunit beta n=1 Tax=A... [more]

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InterPro
Analysis Name: InterProScan on OGS1.0 of Macrocystis pyrifera male
Date Performed: 2022-09-29
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.50.10.20coord: 2..247
e-value: 3.9E-74
score: 252.0
NoneNo IPR availablePANTHERPTHR11774GERANYLGERANYL TRANSFERASE TYPE BETA SUBUNITcoord: 40..246
IPR001330PFTB repeatPFAMPF00432Prenyltranscoord: 146..182
e-value: 0.01
score: 15.6
coord: 103..136
e-value: 2.8E-5
score: 23.7
coord: 198..237
e-value: 6.2E-9
score: 35.5
IPR026872Protein farnesyltransferase subunit betaPANTHERPTHR11774:SF6CHURC1-FNTB READTHROUGH-RELATEDcoord: 40..246
IPR008930Terpenoid cyclases/protein prenyltransferase alpha-alpha toroidSUPERFAMILY48239Terpenoid cyclases/Protein prenyltransferasescoord: 10..245

Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig72997contigM-pyrifera_M_contig72997:370..1110 +
Analyses
This polypeptide is derived from or has results from the following analyses
Analysis NameDate Performed
InterProScan on OGS1.0 of Macrocystis pyrifera male2022-09-29
Diamond blastp: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-16
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Relationships

This polypeptide derives from the following mRNA feature(s):

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig72997.17331.1mRNA_M-pyrifera_M_contig72997.17331.1Macrocystis pyrifera P11B4 malemRNAM-pyrifera_M_contig72997 361..1110 +


Sequences
The following sequences are available for this feature:

polypeptide sequence

>prot_M-pyrifera_M_contig72997.17331.1 ID=prot_M-pyrifera_M_contig72997.17331.1|Name=mRNA_M-pyrifera_M_contig72997.17331.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=247bp
MEMPEYVELMMMDDEGVSSTTSVSQMELEELCARYWAFPSSDVALHRDNH
ERYLSRGLFRRSRSLQGLDASRPWLVYWRVHGLDLLGRRPGPVEREKLRR
VLLEDLQSPTGGFCGGVGQEAHAAPTYSAVLALAVLSEEDGVEGLDREGL
VSFFRRLKAKSGGFAMHVGGEMDVRATYTVVAVSWLTGVLGEEGLMDGVA
EYIGACQTYEGGFGGEPGNEAHGGYTFSAVAALELLGRWDVVDVDAV
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Annotated Terms
The following terms have been associated with this polypeptide:
Vocabulary: INTERPRO
TermDefinition
IPR001330PFTB_repeat
IPR026872FTB
IPR008930Terpenoid_cyclase/PrenylTrfase