mRNA_M-pyrifera_M_contig72433.17222.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig72433.17222.1
Unique NamemRNA_M-pyrifera_M_contig72433.17222.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Match: UPI0008347F52 (NERD domain-containing protein n=1 Tax=Halofilum ochraceum TaxID=1611323 RepID=UPI0008347F52)

HSP 1 Score: 71.6 bits (174), Expect = 4.050e-12
Identity = 38/122 (31.15%), Postives = 64/122 (52.46%), Query Frame = 1
Query:    1 HDQGCPWQKMAVLVGNGNARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQKLKGLEFERVYLVSLDHVYAGPGADDET--KRRLVYVGMTRATDHLMLALHGSGQIIDDLLAA 360
            HD G PW++MAVL     +   + +      +P   L+ +  R +     + V+V T+   KGLEF  V +  ++ +   P  D++   + R++YVGMTRA DHL++  H  G  +D + +A
Sbjct:  478 HDSGIPWREMAVLYRRNKSADAIASALDEAAIPHEALASRRKRSAFDANTETVKVLTMHSSKGLEFRAVAIPGINRM---PLKDEDPTEEARVLYVGMTRAMDHLLITGHDKGGFLDQIRSA 596          
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Match: A0A3B1BA54_9ZZZZ (DNA helicase (Fragment) n=1 Tax=hydrothermal vent metagenome TaxID=652676 RepID=A0A3B1BA54_9ZZZZ)

HSP 1 Score: 68.6 bits (166), Expect = 3.050e-11
Identity = 41/121 (33.88%), Postives = 62/121 (51.24%), Query Frame = 1
Query:    1 HDQGCPWQKMAVLVGNGNARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQKLKGLEFERVYLVSLDHVYAGPGADDETKRRLVYVGMTRATDHLMLALHGSGQIIDDLLAAA 363
            H QG PW +MAVL  +G+    + A   +  +P+A L  K  + +     D V V ++   KGLEF RV ++ +D V      + E   RL+YVGMTRA + L++   G  +    +L  A
Sbjct:  184 HKQGVPWGEMAVLYIDGSGGHAISAQLKKAAIPYAWLGSKKYKSAYNPDLDQVTVLSVYSSKGLEFSRVIMLGIDQVDDSED-NQEDGARLLYVGMTRAQECLLVTSSGMNKYTQRVLEVA 303          
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Match: A0A385Z769_9PSED (DNA helicase n=2 Tax=Pseudomonas cavernae TaxID=2320867 RepID=A0A385Z769_9PSED)

HSP 1 Score: 62.4 bits (150), Expect = 6.990e-9
Identity = 41/124 (33.06%), Postives = 59/124 (47.58%), Query Frame = 1
Query:    1 HDQGCPWQKMAVLVGNGNARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQKLKGLEFERV---YLVSLDHVYAGPGADDETKRRLVYVGMTRATDHLMLALHGSGQIIDDLLAAA 363
            H Q   W ++AVL    +    +     + G+P   ++ K    ++    D V+V T+   KGLEF+ V   ++  L H  A   AD+    RL+YVGMTRATDHL+L    S      L   A
Sbjct:  475 HGQLGDWSQLAVLGRTNDDNVSIVRALQQAGIPVEQVNSKGNNTALDPGADSVKVVTLHSSKGLEFDSVAIPFIGRLPHAKAKSVADEA---RLLYVGMTRATDHLLLTASQSSPFTQRLREVA 595          
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Match: A0A0C4WQJ0_9GAMM (DNA helicase n=3 Tax=Azotobacter chroococcum TaxID=353 RepID=A0A0C4WQJ0_9GAMM)

HSP 1 Score: 62.4 bits (150), Expect = 6.990e-9
Identity = 42/115 (36.52%), Postives = 55/115 (47.83%), Query Frame = 1
Query:    1 HDQGCPWQKMAVLVGNGNARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQKLKGLEFERVYLVSLDHVYAGPGADDET--KRRLVYVGMTRATDHLMLALHGSGQI 339
            H +G  W+ MAVL   G A + +       G+P A L   S      E  D V + TI   KGLEF RV L+    V  G  AD     + RL+YVG+TRA   L++  H S  +
Sbjct:  477 HAEGVAWKDMAVLYPGGGAGQAMGEALAELGIPHAWLG--SGEACCAET-DQVAIMTIHGSKGLEFSRVVLLDASFVAPGEEADSSLPERMRLLYVGITRARQQLLVGFHRSNAV 588          
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Match: A0A0T5ZAD6_9GAMM (DNA helicase n=3 Tax=sulfur-oxidizing symbionts TaxID=32036 RepID=A0A0T5ZAD6_9GAMM)

HSP 1 Score: 62.0 bits (149), Expect = 9.560e-9
Identity = 40/109 (36.70%), Postives = 55/109 (50.46%), Query Frame = 1
Query:    1 HDQGCPWQKMAVLVGNGNARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQKLKGLEFERVYLVSLDHVYAGPGADDETKR----RLVYVGMTRATDHLML 315
            H QG PW+ MAVL   GN    V A      +P   L  K  + +     D V V TI   KGLEF RV ++ +     G   D++ ++    RL+YVGMTRA + L++
Sbjct:  498 HQQGVPWRDMAVLYAAGNQGHGVAAQLRVADIPHVWLGSKQYKSTYDPGDDRVTVLTIHSSKGLEFSRVIILGI-----GQLGDEQARQPQNARLLYVGMTRAQECLLV 601          
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Match: A0A139SNL0_9GAMM (DNA helicase n=2 Tax=Ventosimonas gracilis TaxID=1680762 RepID=A0A139SNL0_9GAMM)

HSP 1 Score: 58.5 bits (140), Expect = 1.300e-8
Identity = 39/113 (34.51%), Postives = 54/113 (47.79%), Query Frame = 1
Query:   28 MAVLVGNGNARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQKLKGLEFERVYLVSLDHVYAGPGADDET---KRRLVYVGMTRATDHLMLALHGSGQIIDDLLA 357
            MAVL   G A  ++     +  +P   L +   RK      D + + TI   KGLEF  V L+    +  G   DD       RL+YVGMTRAT+ L+L+ H   ++   LLA
Sbjct:    1 MAVLYPGGEAGHNMEQALQQAAIPCIWLKDSKTRKDYRVDEDKIPIMTIHSSKGLEFSTVVLLDASFI-PGKEIDDAALTAAMRLLYVGMTRATERLLLSFHRDNELAKALLA 112          
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Match: A0A367FCC4_9ACTN (DNA helicase n=2 Tax=Streptomyces diacarni TaxID=2800381 RepID=A0A367FCC4_9ACTN)

HSP 1 Score: 61.6 bits (148), Expect = 1.340e-8
Identity = 40/124 (32.26%), Postives = 65/124 (52.42%), Query Frame = 1
Query:    1 HDQGCPWQKMAVLVGNG-NARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQKLKGLEFERVYLVSL-----------DHVYAGPGA---DDETKRRLVYVGMTRATDHLMLALHG 327
            HD G P+  MAV V +G +A++    +A    VP  +L +  AR       D VR+ T+ + KGLEF+RV+L S+            + +A P     +++  R L++V  TRA D L+++ +G
Sbjct:  646 HDHGTPYSAMAVSVPDGRSAQEFANVLAREFDVPAVELGKDGARSG----ADAVRIGTMHRFKGLEFQRVFLTSVGEGRVPDQRIEQYRHANPQRYRQEEQRARSLLFVAATRARDELIVSWNG 765          
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Match: A0A4U1KFV5_9GAMM (DNA helicase n=5 Tax=Azotobacter chroococcum TaxID=353 RepID=A0A4U1KFV5_9GAMM)

HSP 1 Score: 60.1 bits (144), Expect = 4.500e-8
Identity = 40/115 (34.78%), Postives = 55/115 (47.83%), Query Frame = 1
Query:    1 HDQGCPWQKMAVLVGNGNARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQKLKGLEFERVYLVSLDHVYAGPGADDET--KRRLVYVGMTRATDHLMLALHGSGQI 339
            H +G  W+ MAVL  +G A + +       G+P   L   S      E  D V + TI   KGLEF RV L+    V  G  AD     + RL+YVG+TRA   L++  H +  +
Sbjct:  477 HAEGVAWRDMAVLYPDGGAGRAMGEALAALGIPHVWLG--SGEACCAET-DQVAIMTIHGSKGLEFSRVVLLDASFVAQGEEADSSLPERMRLLYVGITRARQQLLVGFHRNNAV 588          
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Match: A0A154QH75_9GAMM (DNA helicase n=19 Tax=Rhodanobacteraceae TaxID=1775411 RepID=A0A154QH75_9GAMM)

HSP 1 Score: 60.1 bits (144), Expect = 4.510e-8
Identity = 38/109 (34.86%), Postives = 62/109 (56.88%), Query Frame = 1
Query:    1 HDQGCPWQKMAVLVGNGNARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQKLKGLEFERVYLVSLDHV-YAGPGADDETKRRLVYVGMTRATDHLMLALH 324
            H  G P+ +MAV+  N    + +  V    G+P + L++ + ++++  + D V++ T+   KGLEF  V +  +  +   G G +DE   RL+YV MTRATDHL+L  H
Sbjct:  482 HAHGRPYSEMAVIYRNQWEGEKLHEVLRELGIP-SRLADNAGKQTLFVVEDSVKLVTMHSSKGLEFPFVIIPGIGGLPKEGQGENDEA--RLLYVAMTRATDHLLLIHH 587          
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Match: A0A7K2B2T0_9ACTN (DNA helicase n=1 Tax=Acidimicrobiia bacterium TaxID=2080302 RepID=A0A7K2B2T0_9ACTN)

HSP 1 Score: 59.3 bits (142), Expect = 8.360e-8
Identity = 41/112 (36.61%), Postives = 57/112 (50.89%), Query Frame = 1
Query:   10 GCPWQKMAVLVGNGNARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQKLKGLEFERVYLVSLDHVYAGPGAD----DETKRRLVYVGMTRATDHLMLALHGSG 333
            G     +A+L G+   R  + A      V   DL+++ A     E    VRVAT+Q LKGLEF  V +   +HV+     D    D+ +RRL+YV MTRAT+ L +   G G
Sbjct:  476 GAEADHIAILTGSAELRTQI-ARRVPDAVNAKDLNDRGA-----EPRGAVRVATLQLLKGLEFRHVIVGGANHVWVTDDTDENAQDDQRRRLLYVAMTRATETLTVTYSGEG 581          
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig72433.17222.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
UPI0008347F524.050e-1231.15NERD domain-containing protein n=1 Tax=Halofilum o... [more]
A0A3B1BA54_9ZZZZ3.050e-1133.88DNA helicase (Fragment) n=1 Tax=hydrothermal vent ... [more]
A0A385Z769_9PSED6.990e-933.06DNA helicase n=2 Tax=Pseudomonas cavernae TaxID=23... [more]
A0A0C4WQJ0_9GAMM6.990e-936.52DNA helicase n=3 Tax=Azotobacter chroococcum TaxID... [more]
A0A0T5ZAD6_9GAMM9.560e-936.70DNA helicase n=3 Tax=sulfur-oxidizing symbionts Ta... [more]
A0A139SNL0_9GAMM1.300e-834.51DNA helicase n=2 Tax=Ventosimonas gracilis TaxID=1... [more]
A0A367FCC4_9ACTN1.340e-832.26DNA helicase n=2 Tax=Streptomyces diacarni TaxID=2... [more]
A0A4U1KFV5_9GAMM4.500e-834.78DNA helicase n=5 Tax=Azotobacter chroococcum TaxID... [more]
A0A154QH75_9GAMM4.510e-834.86DNA helicase n=19 Tax=Rhodanobacteraceae TaxID=177... [more]
A0A7K2B2T0_9ACTN8.360e-836.61DNA helicase n=1 Tax=Acidimicrobiia bacterium TaxI... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig72433contigM-pyrifera_M_contig72433:748..1110 -
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeBacteria
Seed ortholog score62.0
Seed ortholog evalue2e-07
Seed eggNOG ortholog1286106.MPL1_07932
KEGG koko:K09384
Hectar predicted targeting categoryother localisation
EggNOG free text desc.Nuclease-related domain
EggNOG OGs1MWW6@1224,1RYRU@1236,4620Z@72273,COG0210@1,COG0210@2,COG1061@1,COG1061@2
COG Functional cat.L
Best tax levelThiotrichales
Best eggNOG OGNA|NA|NA
BRITEko00000
Exons1
Model size363
Cds size336
Stop0
Start1
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816621.166233-CDS-M-pyrifera_M_contig72433:747..10831622816621.166233-CDS-M-pyrifera_M_contig72433:747..1083Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72433 748..1083 -
1692278081.8714108-CDS-M-pyrifera_M_contig72433:747..10831692278081.8714108-CDS-M-pyrifera_M_contig72433:747..1083Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig72433 748..1083 -


The following UTR feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816621.1808932-UTR-M-pyrifera_M_contig72433:1083..11101622816621.1808932-UTR-M-pyrifera_M_contig72433:1083..1110Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig72433 1084..1110 -
1692278081.883505-UTR-M-pyrifera_M_contig72433:1083..11101692278081.883505-UTR-M-pyrifera_M_contig72433:1083..1110Macrocystis pyrifera P11B4 maleUTRM-pyrifera_M_contig72433 1084..1110 -


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig72433.17222.1prot_M-pyrifera_M_contig72433.17222.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig72433 748..1083 -


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig72433.17222.1

>prot_M-pyrifera_M_contig72433.17222.1 ID=prot_M-pyrifera_M_contig72433.17222.1|Name=mRNA_M-pyrifera_M_contig72433.17222.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=112bp
MAVLVGNGNARKHVPAVAGRHGVPFADLSEKSARKSIGEIGDVVRVATIQ
KLKGLEFERVYLVSLDHVYAGPGADDETKRRLVYVGMTRATDHLMLALHG
SGQIIDDLLAAA
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mRNA from alignment at M-pyrifera_M_contig72433:748..1110-

Legend: UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig72433.17222.1 ID=mRNA_M-pyrifera_M_contig72433.17222.1|Name=mRNA_M-pyrifera_M_contig72433.17222.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=363bp|location=Sequence derived from alignment at M-pyrifera_M_contig72433:748..1110- (Macrocystis pyrifera P11B4 male)
CACGACCAGGGTTGCCCCTGGCAGAAAATGGCCGTGCTCGTGGGCAACGG AAATGCGCGCAAGCATGTTCCGGCAGTCGCTGGTCGTCACGGCGTCCCGT TCGCCGATCTTTCGGAGAAGAGCGCTCGGAAGTCCATCGGCGAAATCGGC GACGTCGTACGGGTCGCAACGATTCAGAAGCTCAAGGGCCTTGAGTTCGA GCGCGTGTATCTCGTCAGTCTCGATCACGTGTACGCAGGGCCCGGAGCAG ACGATGAGACGAAACGACGGCTGGTTTATGTCGGGATGACTCGTGCGACC GACCACCTCATGCTCGCATTGCACGGCTCCGGTCAAATCATCGACGATCT TCTGGCCGCCGCA
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig72433:748..1110-

>mRNA_M-pyrifera_M_contig72433.17222.1 ID=mRNA_M-pyrifera_M_contig72433.17222.1|Name=mRNA_M-pyrifera_M_contig72433.17222.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=672bp|location=Sequence derived from alignment at M-pyrifera_M_contig72433:748..1110- (Macrocystis pyrifera P11B4 male)
ATGGCCGTGCTCGTGGGCAACGGAAATGCGCGCAAGCATGTTCCGGCAGT
CGCTGGTCGTCACGGCGTCCCGTTCGCCGATCTTTCGGAGAAGAGCGCTC
GGAAGTCCATCGGCGAAATCGGCGACGTCGTACGGGTCGCAACGATTCAG
AAGCTCAAGGGCCTTGAGTTCGAGCGCGTGTATCTCGTCAGTCTCGATCA
CGTGTACGCAGGGCCCGGAGCAGACGATGAGACGAAACGACGGCTGGTTT
ATGTCGGGATGACTCGTGCGACCGACCACCTCATGCTCGCATTGCACGGC
TCCGGTCAAATCATCGACGATCTTCTGGCCGCCGCAATGGCCGTGCTCGT
GGGCAACGGAAATGCGCGCAAGCATGTTCCGGCAGTCGCTGGTCGTCACG
GCGTCCCGTTCGCCGATCTTTCGGAGAAGAGCGCTCGGAAGTCCATCGGC
GAAATCGGCGACGTCGTACGGGTCGCAACGATTCAGAAGCTCAAGGGCCT
TGAGTTCGAGCGCGTGTATCTCGTCAGTCTCGATCACGTGTACGCAGGGC
CCGGAGCAGACGATGAGACGAAACGACGGCTGGTTTATGTCGGGATGACT
CGTGCGACCGACCACCTCATGCTCGCATTGCACGGCTCCGGTCAAATCAT
CGACGATCTTCTGGCCGCCGCA
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