mRNA_M-pyrifera_M_contig67647.16329.1 (mRNA) Macrocystis pyrifera P11B4 male

You are viewing an mRNA, more information available on the corresponding polypeptide page

Overview
NamemRNA_M-pyrifera_M_contig67647.16329.1
Unique NamemRNA_M-pyrifera_M_contig67647.16329.1
TypemRNA
OrganismMacrocystis pyrifera P11B4 male (Macrocystis pyrifera P11B4 male (Giant kelp))
Homology
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BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Match: H3G703_PHYRM (Ribosomal protein L37 n=17 Tax=Peronosporaceae TaxID=4777 RepID=H3G703_PHYRM)

HSP 1 Score: 121 bits (303), Expect = 1.430e-33
Identity = 66/118 (55.93%), Postives = 74/118 (62.71%), Query Frame = 1 
Query:    4 TKGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSSAPSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSGRMRHLKDMPRRAKNGFREG 357
            TKGTQ+FG RHNK+HT+CRRCG+SSYHIQKS C+SCGYPAAKMRK                                       NW+ KALRRRTTGSGRMRHLK++ RR KNGFREG
Sbjct:    1 TKGTQSFGMRHNKTHTICRRCGRSSYHIQKSTCSSCGYPAAKMRKY--------------------------------------NWSQKALRRRTTGSGRMRHLKNVQRRFKNGFREG 80
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BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Match: A0A6G0WKJ3_9STRA (Uncharacterized protein n=1 Tax=Aphanomyces euteiches TaxID=100861 RepID=A0A6G0WKJ3_9STRA)

HSP 1 Score: 121 bits (304), Expect = 3.480e-33
Identity = 66/118 (55.93%), Postives = 73/118 (61.86%), Query Frame = 1 
Query:    4 TKGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSSAPSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSGRMRHLKDMPRRAKNGFREG 357
            TKGT +FGKRHNKSHT+CRRCGKSSYHIQKS C SCGYPAAKMRK                                       NW+ KALRRRTTG+GRMR+LK +PR+ KNGFREG
Sbjct:    2 TKGTSSFGKRHNKSHTICRRCGKSSYHIQKSTCGSCGYPAAKMRKY--------------------------------------NWSVKALRRRTTGTGRMRYLKTVPRKFKNGFREG 81
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BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Match: A0A4S2N5B7_9PEZI (Ribosomal protein L37 n=1 Tax=Ascodesmis nigricans TaxID=341454 RepID=A0A4S2N5B7_9PEZI)

HSP 1 Score: 119 bits (299), Expect = 8.740e-33
Identity = 65/118 (55.08%), Postives = 78/118 (66.10%), Query Frame = 1 
Query:    4 TKGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSSAPSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSGRMRHLKDMPRRAKNGFREG 357
            TKGT +FGKRHNKSHTLCRRCG+ S+HIQKS C+SCGYPAAK+RKCT   P L P                         P+ +NW+ KA RR+TTG+GRMR+LK + RR KNGF+ G
Sbjct:    2 TKGTSSFGKRHNKSHTLCRRCGRRSFHIQKSTCSSCGYPAAKVRKCTF--PELDP-------------------------PTTNNWSEKAKRRKTTGTGRMRYLKTVSRRFKNGFQIG 92
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BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Match: A0A425D2K7_9STRA (Ribosomal protein L37 n=6 Tax=Saprolegniaceae TaxID=4764 RepID=A0A425D2K7_9STRA)

HSP 1 Score: 118 bits (295), Expect = 3.340e-32
Identity = 63/118 (53.39%), Postives = 73/118 (61.86%), Query Frame = 1 
Query:    4 TKGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSSAPSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSGRMRHLKDMPRRAKNGFREG 357
            TKGT ++GKRHNK+HT+CRRCGKSSYHIQKS C SCGYPAAK+RK                                       NW+ KALRRRTTG+GRMR+LK +PR+ KNGFREG
Sbjct:   13 TKGTSSYGKRHNKTHTICRRCGKSSYHIQKSTCGSCGYPAAKLRKY--------------------------------------NWSVKALRRRTTGTGRMRYLKTVPRKFKNGFREG 92
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BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Match: A0A024UFB8_9STRA (60S ribosomal protein L37 n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A024UFB8_9STRA)

HSP 1 Score: 119 bits (298), Expect = 3.790e-32
Identity = 64/118 (54.24%), Postives = 73/118 (61.86%), Query Frame = 1 
Query:    4 TKGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSSAPSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSGRMRHLKDMPRRAKNGFREG 357
            TKGT ++GKRHNK+HT+CRRCGKSSYHIQKS C SCGYPAAKMRK                                       NW+ KALRRRTTG+GRMR+LK +PR+ KNGFREG
Sbjct:   53 TKGTSSYGKRHNKTHTICRRCGKSSYHIQKSTCGSCGYPAAKMRKY--------------------------------------NWSVKALRRRTTGTGRMRYLKTVPRKFKNGFREG 132
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BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Match: A0A1E1K1L7_9HELO (Related to 60S ribosomal protein L37 n=1 Tax=Rhynchosporium agropyri TaxID=914238 RepID=A0A1E1K1L7_9HELO)

HSP 1 Score: 119 bits (299), Expect = 5.770e-32
Identity = 62/118 (52.54%), Postives = 78/118 (66.10%), Query Frame = 1 
Query:    4 TKGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSSAPSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSGRMRHLKDMPRRAKNGFREG 357
            TKGT +FGKRHNK+HTLCRRCG+ S HIQK  C+SCGYPAAK+R+CT  +        C+G   +          +   +   DNW  KA RR+TTG+GRMRH+K +PRR KNGF+ G
Sbjct:   50 TKGTASFGKRHNKTHTLCRRCGRRSLHIQKHTCSSCGYPAAKIRQCTFYSTLGQEASICEGQQEE----------VQEANKETDNWGEKAKRRKTTGTGRMRHMKGVPRRFKNGFQTG 157
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BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Match: A0A5J4Y7Z1_9CHLO (Ribosomal protein L37 n=1 Tax=Trebouxia sp. A1-2 TaxID=2608996 RepID=A0A5J4Y7Z1_9CHLO)

HSP 1 Score: 117 bits (292), Expect = 7.780e-32
Identity = 62/117 (52.99%), Postives = 74/117 (63.25%), Query Frame = 1 
Query:    7 KGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSSAPSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSGRMRHLKDMPRRAKNGFREG 357
            KGT +FGKR NK+HTLCRRCG+ S+HIQKS C+SCGYPAA++RK                                       NW+AKA+RR+TTG+GRMRHL+DMPRRAKNGFREG
Sbjct:    6 KGTGSFGKRRNKTHTLCRRCGRRSFHIQKSTCSSCGYPAARIRKY--------------------------------------NWSAKAIRRKTTGTGRMRHLRDMPRRAKNGFREG 84
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BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Match: A0A3R7ACW7_9STRA (Uncharacterized protein (Fragment) n=1 Tax=Aphanomyces invadans TaxID=157072 RepID=A0A3R7ACW7_9STRA)

HSP 1 Score: 119 bits (298), Expect = 9.390e-32
Identity = 64/118 (54.24%), Postives = 73/118 (61.86%), Query Frame = 1 
Query:    4 TKGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSSAPSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSGRMRHLKDMPRRAKNGFREG 357
            TKGT ++GKRHNK+HT+CRRCGKSSYHIQKS C SCGYPAAKMRK                                       NW+ KALRRRTTG+GRMR+LK +PR+ KNGFREG
Sbjct:    1 TKGTSSYGKRHNKTHTICRRCGKSSYHIQKSTCGSCGYPAAKMRKY--------------------------------------NWSVKALRRRTTGTGRMRYLKTVPRKFKNGFREG 80
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BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Match: W2K1Q1_PHYPR (Ribosomal protein L37 (Fragment) n=1 Tax=Phytophthora parasitica TaxID=4792 RepID=W2K1Q1_PHYPR)

HSP 1 Score: 115 bits (289), Expect = 3.070e-31
Identity = 63/116 (54.31%), Postives = 72/116 (62.07%), Query Frame = 1 
Query:    4 TKGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSSAPSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSGRMRHLKDMPRRAKNGFR 351
            TKGTQ+FG RHNK+HT+CRRCG+SSYHIQKS C+SCGYPAAKMRK                                       NW+ KALRRRTTG+GRMRHLK++ RR KNGFR
Sbjct:   30 TKGTQSFGMRHNKTHTICRRCGRSSYHIQKSTCSSCGYPAAKMRKF--------------------------------------NWSQKALRRRTTGTGRMRHLKNVQRRFKNGFR 107
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BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Match: A0A2R5G8N7_9STRA (Ribosomal protein L37 n=1 Tax=Hondaea fermentalgiana TaxID=2315210 RepID=A0A2R5G8N7_9STRA)

HSP 1 Score: 115 bits (287), Expect = 3.550e-31
Identity = 62/118 (52.54%), Postives = 71/118 (60.17%), Query Frame = 1 
Query:    4 TKGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSSAPSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSGRMRHLKDMPRRAKNGFREG 357
            TKGTQAFGK+HNKSH LCRRCGKSSYH+QK+ CASCGYP+ KMR+                                        W  KALRRRT G+GRMR+LK +PR+AKNGFREG
Sbjct:    2 TKGTQAFGKKHNKSHVLCRRCGKSSYHVQKATCASCGYPSPKMRRY--------------------------------------QWGKKALRRRTEGTGRMRYLKTIPRKAKNGFREG 81
The following BLAST results are available for this feature:
BLAST of mRNA_M-pyrifera_M_contig67647.16329.1 vs. uniprot
Analysis Date: 2022-09-19 (Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef90)
Total hits: 25
Match NameE-valueIdentityDescription
H3G703_PHYRM1.430e-3355.93Ribosomal protein L37 n=17 Tax=Peronosporaceae Tax... [more]
A0A6G0WKJ3_9STRA3.480e-3355.93Uncharacterized protein n=1 Tax=Aphanomyces euteic... [more]
A0A4S2N5B7_9PEZI8.740e-3355.08Ribosomal protein L37 n=1 Tax=Ascodesmis nigricans... [more]
A0A425D2K7_9STRA3.340e-3253.39Ribosomal protein L37 n=6 Tax=Saprolegniaceae TaxI... [more]
A0A024UFB8_9STRA3.790e-3254.2460S ribosomal protein L37 n=1 Tax=Aphanomyces inva... [more]
A0A1E1K1L7_9HELO5.770e-3252.54Related to 60S ribosomal protein L37 n=1 Tax=Rhync... [more]
A0A5J4Y7Z1_9CHLO7.780e-3252.99Ribosomal protein L37 n=1 Tax=Trebouxia sp. A1-2 T... [more]
A0A3R7ACW7_9STRA9.390e-3254.24Uncharacterized protein (Fragment) n=1 Tax=Aphanom... [more]
W2K1Q1_PHYPR3.070e-3154.31Ribosomal protein L37 (Fragment) n=1 Tax=Phytophth... [more]
A0A2R5G8N7_9STRA3.550e-3152.54Ribosomal protein L37 n=1 Tax=Hondaea fermentalgia... [more]

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Alignments
The following features are aligned
Aligned FeatureFeature TypeAlignment Location
M-pyrifera_M_contig67647contigM-pyrifera_M_contig67647:53..433 +
Analyses
This mRNA is derived from or has results from the following analyses
Analysis NameDate Performed
Diamond blastx: OGS1.0 of Macrocystis pyrifera male vs UniRef902022-09-19
OGS1.0 of Macrocystis pyrifera P11B4 male2021-02-24
Properties
Property NameValue
Taxonomic scopeEukaryota
Seed ortholog score124.8
Seed ortholog evalue2.7e-26
Seed eggNOG ortholog164328.Phyra48748
Preferred nameRPL37
KEGG rclassRC00047,RC00071,RC00080,RC00186,RC00218,RC00242,RC00816,RC01500
KEGG koko:K00128,ko:K02922,ko:K06269,ko:K07891,ko:K09557
KEGG ReactionR00264,R00631,R00710,R00904,R01752,R01986,R02549,R02678,R02940,R02957,R03283,R03869,R04065,R04506,R04903,R05050,R05237,R05238,R05286,R06366,R08146
KEGG Pathwayko00010,ko00053,ko00071,ko00280,ko00310,ko00330,ko00340,ko00380,ko00410,ko00561,ko00620,ko00625,ko00903,ko00981,ko01100,ko01110,ko01120,ko01130,ko03010,ko03015,ko04022,ko04024,ko04113,ko04114,ko04144,ko04218,ko04261,ko04270,ko04390,ko04510,ko04611,ko04720,ko04728,ko04750,ko04810,ko04910,ko04921,ko04931,ko05031,ko05034,ko05168,ko05205,map00010,map00053,map00071,map00280,map00310,map00330,map00340,map00380,map00410,map00561,map00620,map00625,map00903,map00981,map01100,map01110,map01120,map01130,map03010,map03015,map04022,map04024,map04113,map04114,map04144,map04218,map04261,map04270,map04390,map04510,map04611,map04720,map04728,map04750,map04810,map04910,map04921,map04931,map05031,map05034,map05168,map05205
KEGG ModuleM00135,M00177,M00179
Hectar predicted targeting categoryother localisation
GOsGO:0000184,GO:0000448,GO:0000460,GO:0000463,GO:0000466,GO:0000469,GO:0000470,GO:0000478,GO:0000479,GO:0000956,GO:0002181,GO:0003674,GO:0003676,GO:0003723,GO:0003729,GO:0003735,GO:0005198,GO:0005488,GO:0005515,GO:0005575,GO:0005622,GO:0005623,GO:0005737,GO:0005829,GO:0005840,GO:0006139,GO:0006364,GO:0006396,GO:0006401,GO:0006402,GO:0006412,GO:0006413,GO:0006518,GO:0006605,GO:0006612,GO:0006613,GO:0006614,GO:0006725,GO:0006807,GO:0006810,GO:0006886,GO:0008104,GO:0008150,GO:0008152,GO:0008270,GO:0009056,GO:0009057,GO:0009058,GO:0009059,GO:0009892,GO:0009987,GO:0010467,GO:0010468,GO:0010605,GO:0010629,GO:0015031,GO:0015833,GO:0015934,GO:0016070,GO:0016071,GO:0016072,GO:0019222,GO:0019439,GO:0019538,GO:0019838,GO:0019843,GO:0022613,GO:0022625,GO:0022626,GO:0030684,GO:0030687,GO:0032991,GO:0033036,GO:0033365,GO:0034470,GO:0034613,GO:0034641,GO:0034645,GO:0034655,GO:0034660,GO:0042254,GO:0042273,GO:0042886,GO:0043043,GO:0043167,GO:0043169,GO:0043170,GO:0043226,GO:0043228,GO:0043229,GO:0043232,GO:0043603,GO:0043604,GO:0044085,GO:0044237,GO:0044238,GO:0044248,GO:0044249,GO:0044260,GO:0044265,GO:0044267,GO:0044270,GO:0044271,GO:0044391,GO:0044422,GO:0044424,GO:0044444,GO:0044445,GO:0044446,GO:0044464,GO:0045047,GO:0045184,GO:0046483,GO:0046700,GO:0046872,GO:0046907,GO:0046914,GO:0048519,GO:0050789,GO:0051179,GO:0051234,GO:0051641,GO:0051649,GO:0060255,GO:0065007,GO:0070727,GO:0070972,GO:0071702,GO:0071704,GO:0071705,GO:0071840,GO:0072594,GO:0072599,GO:0072657,GO:0090150,GO:0090304,GO:0090305,GO:0090501,GO:0090502,GO:0097159,GO:1901360,GO:1901361,GO:1901363,GO:1901564,GO:1901566,GO:1901575,GO:1901576,GO:1990904
EggNOG free text desc.Ribosomal protein L37
EggNOG OGs3QG8D@4776,COG2126@1,KOG3475@2759
EC1.2.1.3,3.1.3.16
COG Functional cat.J
Best tax levelPeronosporales
Best eggNOG OGNA|NA|NA
BRITEbr01610,ko00000,ko00001,ko00002,ko01000,ko01009,ko03011,ko03019,ko03021,ko03041,ko03110,ko04031,ko04131
Exons2
Model size360
Cds size360
Stop0
Start0
Relationships

The following CDS feature(s) are a part of this mRNA:

Feature NameUnique NameSpeciesTypePosition
1622816567.1495597-CDS-M-pyrifera_M_contig67647:52..2891622816567.1495597-CDS-M-pyrifera_M_contig67647:52..289Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig67647 53..289 +
1692278051.0052135-CDS-M-pyrifera_M_contig67647:52..2891692278051.0052135-CDS-M-pyrifera_M_contig67647:52..289Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig67647 53..289 +
1622816567.1606147-CDS-M-pyrifera_M_contig67647:310..4331622816567.1606147-CDS-M-pyrifera_M_contig67647:310..433Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig67647 311..433 +
1692278051.0201302-CDS-M-pyrifera_M_contig67647:310..4331692278051.0201302-CDS-M-pyrifera_M_contig67647:310..433Macrocystis pyrifera P11B4 maleCDSM-pyrifera_M_contig67647 311..433 +


The following polypeptide feature(s) derives from this mRNA:

Feature NameUnique NameSpeciesTypePosition
mRNA_M-pyrifera_M_contig67647.16329.1prot_M-pyrifera_M_contig67647.16329.1Macrocystis pyrifera P11B4 malepolypeptideM-pyrifera_M_contig67647 53..433 +


Sequences
The following sequences are available for this feature:

protein sequence of mRNA_M-pyrifera_M_contig67647.16329.1

>prot_M-pyrifera_M_contig67647.16329.1 ID=prot_M-pyrifera_M_contig67647.16329.1|Name=mRNA_M-pyrifera_M_contig67647.16329.1|organism=Macrocystis pyrifera P11B4 male|type=polypeptide|length=120bp
QTKGTQAFGKRHNKSHTLCRRCGKSSYHIQKSQCASCGYPAAKMRKCTSS
APSLAPGPRCDGSGLDTWSCVEAAAPLGRTDPSADNWAAKALRRRTTGSG
RMRHLKDMPRRAKNGFREGM
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mRNA from alignment at M-pyrifera_M_contig67647:53..433+

Legend: CDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
>mRNA_M-pyrifera_M_contig67647.16329.1 ID=mRNA_M-pyrifera_M_contig67647.16329.1|Name=mRNA_M-pyrifera_M_contig67647.16329.1|organism=Macrocystis pyrifera P11B4 male|type=mRNA|length=381bp|location=Sequence derived from alignment at M-pyrifera_M_contig67647:53..433+ (Macrocystis pyrifera P11B4 male)
CAGACTAAGGGTACCCAGGCATTTGGAAAGCGTCACAACAAGTCGCACAC
GCTCTGCAGGCGCTGCGGCAAGAGCTCCTACCACATCCAGAAGTCCCAGT
GCGCCTCGTGCGGCTACCCGGCTGCCAAGATGAGGAAGTGTACGTCCTCA
GCCCCCTCGCTTGCGCCCGGTCCCCGTTGCGATGGAAGCGGTCTAGATAC
TTGGTCCTGCGTGGAAGCGGCTGCCCCTCTGGGTCGTGTTAGGCTCAAAG
TGCTGTAGACTGACCCCAGCGCAGACAACTGGGCCGCCAAGGCTCTCCGC
AGGCGCACCACCGGCTCTGGCCGCATGAGGCATCTGAAGGACATGCCCCG
TCGCGCCAAGAACGGATTCCGTGAGGGTATG
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Coding sequence (CDS) from alignment at M-pyrifera_M_contig67647:53..433+

>mRNA_M-pyrifera_M_contig67647.16329.1 ID=mRNA_M-pyrifera_M_contig67647.16329.1|Name=mRNA_M-pyrifera_M_contig67647.16329.1|organism=Macrocystis pyrifera P11B4 male|type=CDS|length=720bp|location=Sequence derived from alignment at M-pyrifera_M_contig67647:53..433+ (Macrocystis pyrifera P11B4 male)
CAGACTAAGGGTACCCAGGCATTTGGAAAGCGTCACAACAAGTCGCACAC
GCTCTGCAGGCGCTGCGGCAAGAGCTCCTACCACATCCAGAAGTCCCAGT
GCGCCTCGTGCGGCTACCCGGCTGCCAAGATGAGGAAGTGTACGTCCTCA
GCCCCCTCGCTTGCGCCCGGTCCCCGTTGCGATGGAAGCGGTCTAGATAC
TTGGTCCTGCGTGGAAGCGGCTGCCCCTCTGGGTCGTCAGACTAAGGGTA
CCCAGGCATTTGGAAAGCGTCACAACAAGTCGCACACGCTCTGCAGGCGC
TGCGGCAAGAGCTCCTACCACATCCAGAAGTCCCAGTGCGCCTCGTGCGG
CTACCCGGCTGCCAAGATGAGGAAGTGTACGTCCTCAGCCCCCTCGCTTG
CGCCCGGTCCCCGTTGCGATGGAAGCGGTCTAGATACTTGGTCCTGCGTG
GAAGCGGCTGCCCCTCTGGGTCGTACTGACCCCAGCGCAGACAACTGGGC
CGCCAAGGCTCTCCGCAGGCGCACCACCGGCTCTGGCCGCATGAGGCATC
TGAAGGACATGCCCCGTCGCGCCAAGAACGGATTCCGTGAGGGTATGACT
GACCCCAGCGCAGACAACTGGGCCGCCAAGGCTCTCCGCAGGCGCACCAC
CGGCTCTGGCCGCATGAGGCATCTGAAGGACATGCCCCGTCGCGCCAAGA
ACGGATTCCGTGAGGGTATG
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